pycom08g09320

Lachrymatory-factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0001280
Physical Location & Seq
Forward (+)
7575581 .. 7576084
504 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g09320.1

Sequence Viewer

Length: 504 bp
ATGCAAGAACCCGCAGAGTCCAGAAAATGGGAGCGCAAGACAACCGCTGTGCTCAAAGCCTCAGCAGCCGAAAAAGTTTGGCCTCTCTTGGCTGATTTCTGCAACTTACACAAAATCTTCCCCAAACTAGCCACATGTTACCAAGTCGAGGGAGTTTCCGGCCAACAGGGTCTGATCCGGTACTGTGAAACTCCTCCTACCAATCCCGCTGATGAGTCGACCGTCAAGTGGGTCAAGGAGAAGCTACTGATGATTGATCCAATCAAACGGTGCATAAGCTATGAGATCATTGAGAATAACATGGGGTTTCACTCTTACGTTGCTACCATGCAAGTAGTGCCTACGAACGACATTGATCAGGATGGTGCTTACGGGTGCAAGATCGATTGGTCTGTTGTTTGTGAACCGGTCGATGGATGGAGATCTGAAAATCTTCAGGAGTTTCTTGAGTCTAATCTTCAATTGGTTGCAAAAACAATGGAGCATGCTCTTCTATCTACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Polyketide_cyc2

Protein Analysis

168

Amino Acids

18.94

Weight (kDa)

5.11

Isoelectric Point (pI)

31.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Polyketide_cyc2 PF10604 22 - 153 4.4e-19 Polyketide cyclase / dehydrase and lipid transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 27
AccI GTMKAC 1 cut(s) 218
AciI CCGC 3 cut(s) 12, 45, 207
AclWI GGATC 2 cut(s) 169, 251
AcoI YGGCCR 1 cut(s) 160
AcuI CTGAAG 1 cut(s) 419
AfaI GTAC 1 cut(s) 182
AfiI CCNNNNNNNGG 4 cut(s) 27, 148, 228, 413
AflIII ACRYGT 1 cut(s) 134
AgeI ACCGGT 1 cut(s) 406
AgsI TTSAA 1 cut(s) 461
AluBI AGCT 2 cut(s) 244, 279
AluI AGCT 2 cut(s) 244, 279
Alw21I GWGCWC 1 cut(s) 54
AlwI GGATC 2 cut(s) 169, 251
AlwNI CAGNNNCTG 1 cut(s) 172
AoxI GGCC 2 cut(s) 80, 160
ApeKI GCWGC 1 cut(s) 65
AsiGI ACCGGT 1 cut(s) 406
Asp700I GAANNNNTTC 1 cut(s) 432
AspLEI GCGC 1 cut(s) 36
Bbv12I GWGCWC 1 cut(s) 54
BbvCI CCTCAGC 1 cut(s) 61
BbvI GCAGC 1 cut(s) 77
BccI CCATC 3 cut(s) 356, 407, 411
BclI TGATCA 1 cut(s) 355
BfaI CTAG 1 cut(s) 128
BglII AGATCT 1 cut(s) 422
BisI GCNGC 1 cut(s) 66
BlsI GCNGC 1 cut(s) 67
Bpu10I CCTNAGC 1 cut(s) 61
BpuEI CTTGAG 1 cut(s) 467
Bsa29I ATCGAT 1 cut(s) 384
BsaBI GATNNNNATC 1 cut(s) 421
BsaWI WCCGGW 2 cut(s) 177, 406
Bsc4I CCNNNNNNNGG 4 cut(s) 27, 148, 228, 413
Bse118I RCCGGY 1 cut(s) 406
Bse8I GATNNNNATC 1 cut(s) 421
BseCI ATCGAT 1 cut(s) 384
BseGI GGATG 2 cut(s) 367, 422
BseJI GATNNNNATC 1 cut(s) 421
BseLI CCNNNNNNNGG 4 cut(s) 27, 148, 228, 413
BseMII CTCAG 1 cut(s) 75
BseRI GAGGAG 1 cut(s) 183
BseXI GCAGC 1 cut(s) 77
Bsh1285I CGRYCG 2 cut(s) 222, 411
BshFI GGCC 2 cut(s) 82, 162
BshTI ACCGGT 1 cut(s) 406
BshVI ATCGAT 1 cut(s) 384
BsiEI CGRYCG 2 cut(s) 222, 411
BsiHKAI GWGCWC 1 cut(s) 54
BsiSI CCGG 3 cut(s) 159, 178, 407
BslI CCNNNNNNNGG 4 cut(s) 27, 148, 228, 413
BsnI GGCC 2 cut(s) 82, 162
Bsp1286I GDGCHC 1 cut(s) 54
Bsp143I GATC 6 cut(s) 174, 256, 285, 355, 381, 422
BspACI CCGC 3 cut(s) 12, 45, 207
BspANI GGCC 2 cut(s) 82, 162
BspCNI CTCAG 1 cut(s) 74
BspDI ATCGAT 1 cut(s) 384
BspPI GGATC 2 cut(s) 169, 251
BspQI GCTCTTC 1 cut(s) 495
BsrFI RCCGGY 1 cut(s) 406
BssAI RCCGGY 1 cut(s) 406
BssMI GATC 6 cut(s) 174, 256, 285, 355, 381, 422
Bst4CI ACNGT 3 cut(s) 185, 223, 270
Bst6I CTCTTC 1 cut(s) 495
BstAPI GCANNNNNTGC 1 cut(s) 337
BstC8I GCNNGC 1 cut(s) 486
BstDEI CTNAG 1 cut(s) 61
BstF5I GGATG 2 cut(s) 367, 422
BstHHI GCGC 1 cut(s) 36
BstKTI GATC 6 cut(s) 177, 259, 288, 358, 384, 425
BstMBI GATC 6 cut(s) 174, 256, 285, 355, 381, 422
BstMCI CGRYCG 2 cut(s) 222, 411
BstMWI GCNNNNNNNGC 2 cut(s) 65, 337
BstNSI RCATGY 2 cut(s) 138, 488
BstV1I GCAGC 1 cut(s) 77
BstX2I RGATCY 1 cut(s) 422
BstYI RGATCY 1 cut(s) 422
Bsu15I ATCGAT 1 cut(s) 384
BsuRI GGCC 2 cut(s) 82, 162
BsuTUI ATCGAT 1 cut(s) 384
BtsCI GGATG 2 cut(s) 367, 422
Cac8I GCNNGC 1 cut(s) 486
CaiI CAGNNNCTG 1 cut(s) 172
CfoI GCGC 1 cut(s) 36
Cfr10I RCCGGY 1 cut(s) 406
ClaI ATCGAT 1 cut(s) 384
Csp6I GTAC 1 cut(s) 181
CspAI ACCGGT 1 cut(s) 406
CviAII CATG 4 cut(s) 135, 301, 328, 485
CviJI RGCY 8 cut(s) 59, 68, 82, 92, 131, 162, 244, 279
CviKI_1 RGCY 8 cut(s) 59, 68, 82, 92, 131, 162, 244, 279
CviQI GTAC 1 cut(s) 181
DdeI CTNAG 1 cut(s) 61
DpnI GATC 6 cut(s) 176, 258, 287, 357, 383, 424
DpnII GATC 6 cut(s) 174, 256, 285, 355, 381, 422
EaeI YGGCCR 1 cut(s) 160
Eam1104I CTCTTC 1 cut(s) 495
EarI CTCTTC 1 cut(s) 495
Eco57I CTGAAG 1 cut(s) 419
FaeI CATG 4 cut(s) 138, 304, 331, 488
FaiI YATR 6 cut(s) 136, 275, 282, 302, 329, 486
FatI CATG 4 cut(s) 134, 300, 327, 484
FauI CCCGC 2 cut(s) 19, 214
FbaI TGATCA 1 cut(s) 355
FblI GTMKAC 1 cut(s) 218
Fnu4HI GCNGC 1 cut(s) 66
FokI GGATG 2 cut(s) 374, 429
Fsp4HI GCNGC 1 cut(s) 66
FspBI CTAG 1 cut(s) 128
GlaI GCGC 1 cut(s) 35
GluI GCNGC 1 cut(s) 66
HaeIII GGCC 2 cut(s) 82, 162
HapII CCGG 3 cut(s) 159, 178, 407
HhaI GCGC 1 cut(s) 36
Hin1II CATG 4 cut(s) 138, 304, 331, 488
Hin6I GCGC 1 cut(s) 34
HinP1I GCGC 1 cut(s) 34
HincII GTYRAC 1 cut(s) 219
HindII GTYRAC 1 cut(s) 219
HinfI GANTC 3 cut(s) 17, 215, 449
HpaII CCGG 3 cut(s) 159, 178, 407
Hpy166II GTNNAC 2 cut(s) 219, 404
Hpy188I TCNGA 2 cut(s) 174, 427
Hpy188III TCNNGA 4 cut(s) 21, 359, 437, 446
Hpy8I GTNNAC 2 cut(s) 219, 404
HpyCH4III ACNGT 3 cut(s) 185, 223, 270
HpyCH4IV ACGT 1 cut(s) 318
HpyCH4V TGCA 6 cut(s) 4, 102, 273, 331, 378, 470
HpyF10VI GCNNNNNNNGC 2 cut(s) 65, 337
HpyF3I CTNAG 1 cut(s) 61
HpySE526I ACGT 1 cut(s) 318
Hsp92II CATG 4 cut(s) 138, 304, 331, 488
HspAI GCGC 1 cut(s) 34
Ksp22I TGATCA 1 cut(s) 355
Kzo9I GATC 6 cut(s) 174, 256, 285, 355, 381, 422
LguI GCTCTTC 1 cut(s) 495
LmnI GCTCC 2 cut(s) 31, 481
LpnPI CCDG 7 cut(s) 34, 152, 172, 191, 344, 420, 422
Lsp1109I GCAGC 1 cut(s) 77
MaeI CTAG 1 cut(s) 128
MaeII ACGT 1 cut(s) 318
MaeIII GTNAC 1 cut(s) 137
MalI GATC 6 cut(s) 176, 258, 287, 357, 383, 424
MboI GATC 6 cut(s) 174, 256, 285, 355, 381, 422
MboII GAAGA 4 cut(s) 109, 425, 449, 482
MfeI CAATTG 1 cut(s) 461
MflI RGATCY 1 cut(s) 422
MhlI GDGCHC 1 cut(s) 54
MluCI AATT 1 cut(s) 461
MlyI GAGTC 3 cut(s) 26, 224, 458
MnlI CCTC 4 cut(s) 70, 93, 142, 204
MroXI GAANNNNTTC 1 cut(s) 432
MseI TTAA 1 cut(s) 502
MspA1I CMGCKG 2 cut(s) 47, 209
MspI CCGG 3 cut(s) 159, 178, 407
MunI CAATTG 1 cut(s) 461
MwoI GCNNNNNNNGC 2 cut(s) 65, 337
NdeII GATC 6 cut(s) 174, 256, 285, 355, 381, 422
NlaIII CATG 4 cut(s) 138, 304, 331, 488
NspI RCATGY 2 cut(s) 138, 488
PaeI GCATGC 1 cut(s) 488
PciI ACATGT 1 cut(s) 134
PciSI GCTCTTC 1 cut(s) 495
PdmI GAANNNNTTC 1 cut(s) 432
PflMI CCANNNNNTGG 1 cut(s) 27
PinAI ACCGGT 1 cut(s) 406
PkrI GCNGC 1 cut(s) 67
PleI GAGTC 3 cut(s) 25, 223, 457
PpsI GAGTC 3 cut(s) 25, 223, 457
PscI ACATGT 1 cut(s) 134
PstNI CAGNNNCTG 1 cut(s) 172
PsuI RGATCY 1 cut(s) 422
RsaI GTAC 1 cut(s) 182
RsaNI GTAC 1 cut(s) 181
SalI GTCGAC 1 cut(s) 217
SapI GCTCTTC 1 cut(s) 495
SaqAI TTAA 1 cut(s) 502
SatI GCNGC 1 cut(s) 66
Sau3AI GATC 6 cut(s) 174, 256, 285, 355, 381, 422
SchI GAGTC 3 cut(s) 26, 224, 458
SduI GDGCHC 1 cut(s) 54
SetI ASST 3 cut(s) 246, 281, 321
SmlI CTYRAG 1 cut(s) 446
SmoI CTYRAG 1 cut(s) 446
SphI GCATGC 1 cut(s) 488
Sse9I AATT 1 cut(s) 461
SsiI CCGC 3 cut(s) 12, 45, 207
SspMI CTAG 1 cut(s) 128
TaaI ACNGT 3 cut(s) 185, 223, 270
TaiI ACGT 1 cut(s) 321
TaqI TCGA 4 cut(s) 147, 218, 384, 411
TasI AATT 1 cut(s) 461
Tru1I TTAA 1 cut(s) 502
Tru9I TTAA 1 cut(s) 502
TseI GCWGC 1 cut(s) 65
Van91I CCANNNNNTGG 1 cut(s) 27
XceI RCATGY 2 cut(s) 138, 488
XmiI GTMKAC 1 cut(s) 218
XmnI GAANNNNTTC 1 cut(s) 432
XspI CTAG 1 cut(s) 128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.