Rroxscaffold_7G00161730

Lachrymatory-factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0001280
Physical Location & Seq
Reverse (-)
4423085 .. 4425288
2204 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00161730.1

Sequence Viewer

Length: 627 bp
ATGTGCGGATATCCGTACCTAAGCAAAATCATATACGAAGAAGCTTTATGTGGTTGCAATGAGCTAGATAGCCTTCTCCCTTTGACAGTTCTTTCTCTTACTTCTCTCTGCATCTTTCTCATGGCATCAGAGGTTCAAGCACAGCCGAAATGGGAAGGTAAAACCTCAGCCGAGCTCAAAAGCTCACCAGCACAACAGGTCTGGCCTTTCCTTGCCGATTTCTGCAACTTACACAAAGTCTTCGCAGGCCTTGAAACTTGCTACCAAGTCGAGGGAGTCCCCGGCCAGCCGGGCCTGATCCGCTACTGCGCCGGACCGGCTACCGATAACGACGAGACCACCGTTAAGTGGGCCAAAGAGAAGCTGATCGCGGTTGATCCGATCAAACGTTGCTTCAGCTACGAGATGCTTGAGAATAACAAGGGGTATAAGTCGTATGTTGCAACCATGCAAGTGGTTCCGGTGATGAACGACGATGAAGGGTGCATGATCGAGTGGTCGTTTGTTTCTGATCCGGTCGAAGGGTGGGGATTGGAGGACTTTCAGTCTTATCGTGAGTCGAGTCTACAAGGGATGGCAAAGAAGATTGAGGATGCTCTTCAGTCTAGTACAAGTGCTACTGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Polyketide_cyc2

Protein Analysis

208

Amino Acids

23.12

Weight (kDa)

4.51

Isoelectric Point (pI)

39.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Polyketide_cyc2 PF10604 62 - 197 2.8e-18 Polyketide cyclase / dehydrase and lipid transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 565
AccII CGCG 1 cut(s) 371
AciI CCGC 3 cut(s) 6, 301, 371
AclI AACGTT 1 cut(s) 388
AclWI GGATC 3 cut(s) 292, 371, 506
AcoI YGGCCR 1 cut(s) 283
AcuI CTGAAG 2 cut(s) 379, 584
AfaI GTAC 2 cut(s) 17, 610
AfiI CCNNNNNNNGG 3 cut(s) 271, 348, 521
AgsI TTSAA 2 cut(s) 137, 254
AhdI GACNNNNNGTC 1 cut(s) 544
AluBI AGCT 6 cut(s) 44, 64, 175, 183, 364, 399
AluI AGCT 6 cut(s) 44, 64, 175, 183, 364, 399
Alw21I GWGCWC 1 cut(s) 177
Alw26I GTCTC 1 cut(s) 329
AlwI GGATC 3 cut(s) 292, 371, 506
AoxI GGCC 5 cut(s) 203, 247, 283, 292, 351
AspLEI GCGC 1 cut(s) 311
AspS9I GGNCC 3 cut(s) 292, 314, 351
AsuC2I CCSGG 2 cut(s) 282, 291
AsuHPI GGTGA 2 cut(s) 177, 475
AvaII GGWCC 1 cut(s) 314
BanII GRGCYC 1 cut(s) 177
BbsI GAAGAC 1 cut(s) 232
Bbv12I GWGCWC 1 cut(s) 177
BbvCI CCTCAGC 1 cut(s) 166
BccI CCATC 1 cut(s) 568
BcnI CCSGG 2 cut(s) 282, 291
BcoDI GTCTC 1 cut(s) 329
BfaI CTAG 2 cut(s) 65, 606
BglI GCCNNNNNGGC 2 cut(s) 291, 317
Bme1390I CCNGG 2 cut(s) 282, 291
Bme18I GGWCC 1 cut(s) 314
BmeRI GACNNNNNGTC 1 cut(s) 544
BmgT120I GGNCC 3 cut(s) 292, 314, 351
BmiI GGNNCC 1 cut(s) 459
BmrFI CCNGG 2 cut(s) 282, 291
BmsI GCATC 4 cut(s) 120, 134, 396, 583
BpiI GAAGAC 1 cut(s) 232
Bpu10I CCTNAGC 2 cut(s) 20, 166
BpuEI CTTGAG 1 cut(s) 431
BpuMI CCSGG 2 cut(s) 282, 291
BsaI GGTCTC 1 cut(s) 329
BsaJI CCNNGG 1 cut(s) 280
BsaWI WCCGGW 2 cut(s) 460, 514
Bsc4I CCNNNNNNNGG 3 cut(s) 271, 348, 521
Bse118I RCCGGY 1 cut(s) 316
Bse3DI GCAATG 1 cut(s) 64
BseDI CCNNGG 1 cut(s) 280
BseGI GGATG 2 cut(s) 579, 598
BseLI CCNNNNNNNGG 3 cut(s) 271, 348, 521
BseMI GCAATG 1 cut(s) 64
BseMII CTCAG 1 cut(s) 180
Bsh1236I CGCG 1 cut(s) 371
Bsh1285I CGRYCG 1 cut(s) 519
BshFI GGCC 5 cut(s) 205, 249, 285, 294, 353
BsiEI CGRYCG 1 cut(s) 519
BsiHKAI GWGCWC 1 cut(s) 177
BsiSI CCGG 6 cut(s) 282, 290, 312, 317, 461, 515
BslFI GGGAC 1 cut(s) 263
BslI CCNNNNNNNGG 3 cut(s) 271, 348, 521
BsmAI GTCTC 1 cut(s) 329
BsmFI GGGAC 1 cut(s) 263
BsnI GGCC 5 cut(s) 205, 249, 285, 294, 353
Bso31I GGTCTC 1 cut(s) 329
Bsp1286I GDGCHC 1 cut(s) 177
Bsp143I GATC 6 cut(s) 297, 366, 376, 381, 489, 511
BspACI CCGC 3 cut(s) 6, 301, 371
BspANI GGCC 5 cut(s) 205, 249, 285, 294, 353
BspCNI CTCAG 1 cut(s) 179
BspFNI CGCG 1 cut(s) 371
BspLI GGNNCC 1 cut(s) 459
BspPI GGATC 3 cut(s) 292, 371, 506
BspQI GCTCTTC 1 cut(s) 603
BspTNI GGTCTC 1 cut(s) 329
BsrDI GCAATG 1 cut(s) 64
BsrFI RCCGGY 1 cut(s) 316
BssAI RCCGGY 1 cut(s) 316
BssECI CCNNGG 1 cut(s) 280
BssMI GATC 6 cut(s) 297, 366, 376, 381, 489, 511
Bst4CI ACNGT 3 cut(s) 88, 343, 622
Bst6I CTCTTC 1 cut(s) 603
BstC8I GCNNGC 2 cut(s) 247, 287
BstDEI CTNAG 2 cut(s) 20, 166
BstF5I GGATG 2 cut(s) 579, 598
BstFNI CGCG 1 cut(s) 371
BstHHI GCGC 1 cut(s) 311
BstKTI GATC 6 cut(s) 300, 369, 379, 384, 492, 514
BstMAI GTCTC 1 cut(s) 329
BstMBI GATC 6 cut(s) 297, 366, 376, 381, 489, 511
BstMCI CGRYCG 1 cut(s) 519
BstMWI GCNNNNNNNGC 3 cut(s) 291, 300, 317
BstSCI CCNGG 2 cut(s) 280, 289
BstUI CGCG 1 cut(s) 371
BstV2I GAAGAC 1 cut(s) 232
BstXI CCANNNNNNTGG 1 cut(s) 454
BsuRI GGCC 5 cut(s) 205, 249, 285, 294, 353
BtsCI GGATG 2 cut(s) 579, 598
Cac8I GCNNGC 2 cut(s) 247, 287
CfoI GCGC 1 cut(s) 311
Cfr10I RCCGGY 1 cut(s) 316
Cfr13I GGNCC 3 cut(s) 292, 314, 351
CpoI CGGWCCG 1 cut(s) 314
Csp6I GTAC 2 cut(s) 16, 609
CspI CGGWCCG 1 cut(s) 314
CviAII CATG 3 cut(s) 121, 448, 487
CviQI GTAC 2 cut(s) 16, 609
DdeI CTNAG 2 cut(s) 20, 166
DpnI GATC 6 cut(s) 299, 368, 378, 383, 491, 513
DpnII GATC 6 cut(s) 297, 366, 376, 381, 489, 511
DriI GACNNNNNGTC 1 cut(s) 544
EaeI YGGCCR 1 cut(s) 283
Eam1104I CTCTTC 1 cut(s) 603
Eam1105I GACNNNNNGTC 1 cut(s) 544
EarI CTCTTC 1 cut(s) 603
Ecl136II GAGCTC 1 cut(s) 175
Eco147I AGGCCT 1 cut(s) 249
Eco24I GRGCYC 1 cut(s) 177
Eco31I GGTCTC 1 cut(s) 329
Eco32I GATATC 1 cut(s) 11
Eco47I GGWCC 1 cut(s) 314
Eco53kI GAGCTC 1 cut(s) 175
Eco57I CTGAAG 2 cut(s) 379, 584
EcoICRI GAGCTC 1 cut(s) 175
EcoRV GATATC 1 cut(s) 11
EcoT38I GRGCYC 1 cut(s) 177
FaeI CATG 3 cut(s) 124, 451, 490
FaiI YATR 8 cut(s) 32, 34, 49, 122, 429, 438, 449, 488
FaqI GGGAC 1 cut(s) 263
FatI CATG 3 cut(s) 120, 447, 486
FblI GTMKAC 1 cut(s) 565
FokI GGATG 2 cut(s) 586, 605
FriOI GRGCYC 1 cut(s) 177
FspBI CTAG 2 cut(s) 65, 606
GlaI GCGC 1 cut(s) 310
HaeIII GGCC 5 cut(s) 205, 249, 285, 294, 353
HapII CCGG 6 cut(s) 282, 290, 312, 317, 461, 515
HhaI GCGC 1 cut(s) 311
Hin1II CATG 3 cut(s) 124, 451, 490
Hin6I GCGC 1 cut(s) 309
HinP1I GCGC 1 cut(s) 309
HindIII AAGCTT 1 cut(s) 42
HinfI GANTC 3 cut(s) 276, 557, 562
HpaII CCGG 6 cut(s) 282, 290, 312, 317, 461, 515
HphI GGTGA 2 cut(s) 177, 475
Hpy166II GTNNAC 1 cut(s) 566
Hpy188I TCNGA 3 cut(s) 130, 381, 511
Hpy188III TCNNGA 1 cut(s) 554
Hpy8I GTNNAC 1 cut(s) 566
Hpy99I CGWCG 2 cut(s) 335, 476
HpyAV CCTTC 4 cut(s) 83, 149, 473, 515
HpyCH4III ACNGT 3 cut(s) 88, 343, 622
HpyCH4IV ACGT 1 cut(s) 388
HpyCH4V TGCA 6 cut(s) 57, 111, 225, 443, 451, 486
HpyF10VI GCNNNNNNNGC 3 cut(s) 291, 300, 317
HpyF3I CTNAG 2 cut(s) 20, 166
HpySE526I ACGT 1 cut(s) 388
Hsp92II CATG 3 cut(s) 124, 451, 490
HspAI GCGC 1 cut(s) 309
Kzo9I GATC 6 cut(s) 297, 366, 376, 381, 489, 511
LguI GCTCTTC 1 cut(s) 603
LweI GCATC 4 cut(s) 120, 134, 396, 583
MaeI CTAG 2 cut(s) 65, 606
MaeII ACGT 1 cut(s) 388
MalI GATC 6 cut(s) 299, 368, 378, 383, 491, 513
MboI GATC 6 cut(s) 297, 366, 376, 381, 489, 511
MboII GAAGA 4 cut(s) 50, 232, 590, 595
MhlI GDGCHC 1 cut(s) 177
MlyI GAGTC 3 cut(s) 285, 566, 571
MnlI CCTC 5 cut(s) 124, 175, 265, 529, 583
MseI TTAA 1 cut(s) 345
MslI CAYNNNNRTG 1 cut(s) 452
MspI CCGG 6 cut(s) 282, 290, 312, 317, 461, 515
MspR9I CCNGG 2 cut(s) 282, 291
MvnI CGCG 1 cut(s) 371
MwoI GCNNNNNNNGC 3 cut(s) 291, 300, 317
NciI CCSGG 2 cut(s) 282, 291
NdeII GATC 6 cut(s) 297, 366, 376, 381, 489, 511
NlaIII CATG 3 cut(s) 124, 451, 490
NlaIV GGNNCC 1 cut(s) 459
NmeAIII GCCGAG 1 cut(s) 196
PceI AGGCCT 1 cut(s) 249
PciSI GCTCTTC 1 cut(s) 603
PcsI WCGNNNNNNNCGW 1 cut(s) 339
PleI GAGTC 3 cut(s) 284, 565, 570
PpsI GAGTC 3 cut(s) 284, 565, 570
Psp124BI GAGCTC 1 cut(s) 177
Psp1406I AACGTT 1 cut(s) 388
PspN4I GGNNCC 1 cut(s) 459
PspPI GGNCC 3 cut(s) 292, 314, 351
RsaI GTAC 2 cut(s) 17, 610
RsaNI GTAC 2 cut(s) 16, 609
RseI CAYNNNNRTG 1 cut(s) 452
Rsr2I CGGWCCG 1 cut(s) 314
RsrII CGGWCCG 1 cut(s) 314
SacI GAGCTC 1 cut(s) 177
SapI GCTCTTC 1 cut(s) 603
SaqAI TTAA 1 cut(s) 345
Sau3AI GATC 6 cut(s) 297, 366, 376, 381, 489, 511
Sau96I GGNCC 3 cut(s) 292, 314, 351
SchI GAGTC 3 cut(s) 285, 566, 571
ScrFI CCNGG 2 cut(s) 282, 291
SduI GDGCHC 1 cut(s) 177
SfaNI GCATC 4 cut(s) 120, 134, 396, 583
SfiI GGCCNNNNNGGCC 1 cut(s) 291
SinI GGWCC 1 cut(s) 314
SmiMI CAYNNNNRTG 1 cut(s) 452
SmlI CTYRAG 1 cut(s) 410
SmoI CTYRAG 1 cut(s) 410
SseBI AGGCCT 1 cut(s) 249
SsiI CCGC 3 cut(s) 6, 301, 371
SspMI CTAG 2 cut(s) 65, 606
SstI GAGCTC 1 cut(s) 177
StuI AGGCCT 1 cut(s) 249
StyD4I CCNGG 2 cut(s) 280, 289
TaaI ACNGT 3 cut(s) 88, 343, 622
TaiI ACGT 1 cut(s) 391
TaqI TCGA 4 cut(s) 270, 492, 519, 560
TatI WGTACW 1 cut(s) 608
Tru1I TTAA 1 cut(s) 345
Tru9I TTAA 1 cut(s) 345
TspDTI ATGAA 2 cut(s) 482, 492
VpaK11BI GGWCC 1 cut(s) 314
XmiI GTMKAC 1 cut(s) 565
XspI CTAG 2 cut(s) 65, 606
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.