Rh1DG281300

Lachrymatory-factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0001280
Physical Location & Seq
Forward (+)
50265143 .. 50265670
528 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG281300.1

Sequence Viewer

Length: 528 bp
ATGGAGGAAGAAAGCCAGCTACCAAAATGGGAAGGCAAAACGTCAGCTGAGCTGAAAGGCCCGGCAGCAGAAGAAGTTTGGCCTCTCTTGGCAGATTTCTGCAACATACATAAGTGGTTCCAAAGTCTTGAGACCAGTTATCATGTGGAGGGAGTCCCCGGCCAACCTGGCCTGGTTCGCTACTGCGGGCAACGTGACCATGATGATGACGAAGATGATCAGACGTCGGACACGATCAAGTTCTCAGCTAAAGAGAAGCTACTGATGATCGATCCAGTCAGGCGGTGTTTGAGCTACGAGATACTTGACAACAACTGGGGGCTCAAGTCATATGTTGCAACCATGCAAGTGTTGGATCTGGATGGGAATAACGACCAAAGCCGCCGCGGGTGCAAGATTGAGTGGTCGTTTGTTTGTGATCCGATTGAGGGATGGAGGTTTGGAGATTACGTATCTCTTCTCGACTCTTGTCTGCTATCCATGGCAAAAAAGATGGAAAATGATGCCTTTCCATCTACTACTACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Polyketide_cyc2

Protein Analysis

175

Amino Acids

19.99

Weight (kDa)

4.53

Isoelectric Point (pI)

51.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Polyketide_cyc2 PF10604 22 - 148 1.6e-15 Polyketide cyclase / dehydrase and lipid transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 227
AccII CGCG 1 cut(s) 387
AciI CCGC 5 cut(s) 186, 283, 382, 385, 387
AclWI GGATC 3 cut(s) 266, 363, 413
AcoI YGGCCR 1 cut(s) 160
AcyI GRCGYC 1 cut(s) 224
AfiI CCNNNNNNNGG 1 cut(s) 428
AjnI CCWGG 2 cut(s) 166, 171
AluBI AGCT 6 cut(s) 19, 47, 52, 248, 259, 294
AluI AGCT 6 cut(s) 19, 47, 52, 248, 259, 294
Alw26I GTCTC 1 cut(s) 125
AlwI GGATC 3 cut(s) 266, 363, 413
AoxI GGCC 4 cut(s) 58, 80, 160, 169
ApeKI GCWGC 1 cut(s) 65
AspS9I GGNCC 1 cut(s) 59
AsuC2I CCSGG 2 cut(s) 62, 159
BanII GRGCYC 1 cut(s) 324
BbvI GCAGC 1 cut(s) 77
BccI CCATC 4 cut(s) 356, 426, 487, 520
BciT130I CCWGG 2 cut(s) 168, 173
BclI TGATCA 1 cut(s) 217
BcnI CCSGG 2 cut(s) 62, 159
BcoDI GTCTC 1 cut(s) 125
BfaI CTAG 1 cut(s) 526
BglI GCCNNNNNGGC 1 cut(s) 168
BisI GCNGC 3 cut(s) 66, 382, 385
BlpI GCTNAGC 1 cut(s) 48
BlsI GCNGC 3 cut(s) 67, 383, 386
Bme1390I CCNGG 4 cut(s) 62, 159, 168, 173
BmgT120I GGNCC 1 cut(s) 59
BmiI GGNNCC 1 cut(s) 119
BmrFI CCNGG 4 cut(s) 62, 159, 168, 173
BmrI ACTGGG 1 cut(s) 325
BmsI GCATC 1 cut(s) 493
BmuI ACTGGG 1 cut(s) 325
BoxI GACNNNNGTC 1 cut(s) 468
Bpu1102I GCTNAGC 1 cut(s) 48
BpuEI CTTGAG 2 cut(s) 149, 308
BpuMI CCSGG 2 cut(s) 62, 159
Bsa29I ATCGAT 1 cut(s) 270
BsaAI YACGTR 1 cut(s) 451
BsaHI GRCGYC 1 cut(s) 224
BsaI GGTCTC 1 cut(s) 125
BsaJI CCNNGG 3 cut(s) 157, 385, 480
BsaXI ACNNNNNCTCC 2 cut(s) 435, 465
Bsc4I CCNNNNNNNGG 1 cut(s) 428
Bse1I ACTGG 3 cut(s) 135, 275, 320
BseBI CCWGG 2 cut(s) 168, 173
BseCI ATCGAT 1 cut(s) 270
BseDI CCNNGG 3 cut(s) 157, 385, 480
BseGI GGATG 2 cut(s) 367, 437
BseLI CCNNNNNNNGG 1 cut(s) 428
BseMII CTCAG 2 cut(s) 39, 258
BseNI ACTGG 3 cut(s) 135, 275, 320
BseXI GCAGC 1 cut(s) 77
Bsh1236I CGCG 1 cut(s) 387
BshFI GGCC 4 cut(s) 60, 82, 162, 171
BshVI ATCGAT 1 cut(s) 270
BsiSI CCGG 2 cut(s) 62, 159
BslFI GGGAC 1 cut(s) 140
BslI CCNNNNNNNGG 1 cut(s) 428
BsmAI GTCTC 1 cut(s) 125
BsmFI GGGAC 1 cut(s) 140
BsnI GGCC 4 cut(s) 60, 82, 162, 171
Bso31I GGTCTC 1 cut(s) 125
Bsp1286I GDGCHC 1 cut(s) 324
Bsp143I GATC 6 cut(s) 217, 234, 267, 271, 355, 418
Bsp1720I GCTNAGC 1 cut(s) 48
Bsp19I CCATGG 1 cut(s) 480
BspACI CCGC 5 cut(s) 186, 283, 382, 385, 387
BspANI GGCC 4 cut(s) 60, 82, 162, 171
BspCNI CTCAG 2 cut(s) 40, 257
BspDI ATCGAT 1 cut(s) 270
BspFNI CGCG 1 cut(s) 387
BspLI GGNNCC 1 cut(s) 119
BspPI GGATC 3 cut(s) 266, 363, 413
BspTNI GGTCTC 1 cut(s) 125
BsrI ACTGG 3 cut(s) 135, 275, 320
BssECI CCNNGG 3 cut(s) 157, 385, 480
BssMI GATC 6 cut(s) 217, 234, 267, 271, 355, 418
BssNI GRCGYC 1 cut(s) 224
BssT1I CCWWGG 1 cut(s) 480
Bst2UI CCWGG 2 cut(s) 168, 173
Bst6I CTCTTC 1 cut(s) 462
BstACI GRCGYC 1 cut(s) 224
BstBAI YACGTR 1 cut(s) 451
BstC8I GCNNGC 2 cut(s) 17, 188
BstDEI CTNAG 2 cut(s) 48, 244
BstDSI CCRYGG 2 cut(s) 385, 480
BstF5I GGATG 2 cut(s) 367, 437
BstFNI CGCG 1 cut(s) 387
BstKTI GATC 6 cut(s) 220, 237, 270, 274, 358, 421
BstMAI GTCTC 1 cut(s) 125
BstMBI GATC 6 cut(s) 217, 234, 267, 271, 355, 418
BstMWI GCNNNNNNNGC 3 cut(s) 168, 177, 390
BstNI CCWGG 2 cut(s) 168, 173
BstPAI GACNNNNGTC 1 cut(s) 468
BstSCI CCNGG 4 cut(s) 60, 157, 166, 171
BstSNI TACGTA 1 cut(s) 451
BstUI CGCG 1 cut(s) 387
BstV1I GCAGC 1 cut(s) 77
BstX2I RGATCY 1 cut(s) 355
BstYI RGATCY 1 cut(s) 355
Bsu15I ATCGAT 1 cut(s) 270
BsuRI GGCC 4 cut(s) 60, 82, 162, 171
BsuTUI ATCGAT 1 cut(s) 270
BtgI CCRYGG 2 cut(s) 385, 480
BtsCI GGATG 2 cut(s) 367, 437
Cac8I GCNNGC 2 cut(s) 17, 188
Cfr13I GGNCC 1 cut(s) 59
Cfr42I CCGCGG 1 cut(s) 388
ClaI ATCGAT 1 cut(s) 270
CviAII CATG 4 cut(s) 143, 200, 343, 481
DdeI CTNAG 2 cut(s) 48, 244
DpnI GATC 6 cut(s) 219, 236, 269, 273, 357, 420
DpnII GATC 6 cut(s) 217, 234, 267, 271, 355, 418
EaeI YGGCCR 1 cut(s) 160
Eam1104I CTCTTC 1 cut(s) 462
EarI CTCTTC 1 cut(s) 462
Eco105I TACGTA 1 cut(s) 451
Eco130I CCWWGG 1 cut(s) 480
Eco24I GRGCYC 1 cut(s) 324
Eco31I GGTCTC 1 cut(s) 125
EcoRII CCWGG 2 cut(s) 166, 171
EcoT14I CCWWGG 1 cut(s) 480
EcoT38I GRGCYC 1 cut(s) 324
ErhI CCWWGG 1 cut(s) 480
FaeI CATG 4 cut(s) 146, 203, 346, 484
FaiI YATR 8 cut(s) 107, 111, 144, 201, 331, 333, 344, 482
FaqI GGGAC 1 cut(s) 140
FatI CATG 4 cut(s) 142, 199, 342, 480
FauI CCCGC 2 cut(s) 179, 380
FauNDI CATATG 1 cut(s) 331
FbaI TGATCA 1 cut(s) 217
Fnu4HI GCNGC 3 cut(s) 66, 382, 385
FokI GGATG 2 cut(s) 374, 444
FriOI GRGCYC 1 cut(s) 324
Fsp4HI GCNGC 3 cut(s) 66, 382, 385
FspBI CTAG 1 cut(s) 526
GluI GCNGC 3 cut(s) 66, 382, 385
HaeIII GGCC 4 cut(s) 60, 82, 162, 171
HapII CCGG 2 cut(s) 62, 159
Hin1I GRCGYC 1 cut(s) 224
Hin1II CATG 4 cut(s) 146, 203, 346, 484
HinfI GANTC 2 cut(s) 153, 464
HpaII CCGG 2 cut(s) 62, 159
Hpy188I TCNGA 3 cut(s) 222, 229, 423
Hpy188III TCNNGA 3 cut(s) 128, 359, 461
Hpy99I CGWCG 1 cut(s) 229
HpyAV CCTTC 1 cut(s) 26
HpyCH4IV ACGT 4 cut(s) 41, 193, 224, 450
HpyCH4V TGCA 4 cut(s) 102, 338, 346, 393
HpyF10VI GCNNNNNNNGC 3 cut(s) 168, 177, 390
HpyF3I CTNAG 2 cut(s) 48, 244
HpySE526I ACGT 4 cut(s) 41, 193, 224, 450
Hsp92I GRCGYC 1 cut(s) 224
Hsp92II CATG 4 cut(s) 146, 203, 346, 484
Ksp22I TGATCA 1 cut(s) 217
KspI CCGCGG 1 cut(s) 388
Kzo9I GATC 6 cut(s) 217, 234, 267, 271, 355, 418
Lsp1109I GCAGC 1 cut(s) 77
LweI GCATC 1 cut(s) 493
MaeI CTAG 1 cut(s) 526
MaeII ACGT 4 cut(s) 41, 193, 224, 450
MaeIII GTNAC 1 cut(s) 194
MalI GATC 6 cut(s) 219, 236, 269, 273, 357, 420
MboI GATC 6 cut(s) 217, 234, 267, 271, 355, 418
MboII GAAGA 4 cut(s) 20, 83, 224, 449
MflI RGATCY 1 cut(s) 355
MhlI GDGCHC 1 cut(s) 324
MlyI GAGTC 2 cut(s) 162, 458
MmeI TCCRAC 2 cut(s) 207, 333
MnlI CCTC 4 cut(s) 93, 142, 421, 429
MslI CAYNNNNRTG 2 cut(s) 204, 347
MspA1I CMGCKG 2 cut(s) 47, 387
MspI CCGG 2 cut(s) 62, 159
MspR9I CCNGG 4 cut(s) 62, 159, 168, 173
MvaI CCWGG 2 cut(s) 168, 173
MvnI CGCG 1 cut(s) 387
MwoI GCNNNNNNNGC 3 cut(s) 168, 177, 390
NciI CCSGG 2 cut(s) 62, 159
NcoI CCATGG 1 cut(s) 480
NdeI CATATG 1 cut(s) 331
NdeII GATC 6 cut(s) 217, 234, 267, 271, 355, 418
NlaIII CATG 4 cut(s) 146, 203, 346, 484
NlaIV GGNNCC 1 cut(s) 119
NmuCI GTSAC 1 cut(s) 194
PcsI WCGNNNNNNNCGW 1 cut(s) 230
PkrI GCNGC 3 cut(s) 67, 383, 386
PleI GAGTC 2 cut(s) 161, 458
PpsI GAGTC 2 cut(s) 161, 458
Ppu21I YACGTR 1 cut(s) 451
PshAI GACNNNNGTC 1 cut(s) 468
Psp6I CCWGG 2 cut(s) 166, 171
PspGI CCWGG 2 cut(s) 166, 171
PspN4I GGNNCC 1 cut(s) 119
PspPI GGNCC 1 cut(s) 59
PsuI RGATCY 1 cut(s) 355
PvuII CAGCTG 1 cut(s) 47
RseI CAYNNNNRTG 2 cut(s) 204, 347
SacII CCGCGG 1 cut(s) 388
SatI GCNGC 3 cut(s) 66, 382, 385
Sau3AI GATC 6 cut(s) 217, 234, 267, 271, 355, 418
Sau96I GGNCC 1 cut(s) 59
SchI GAGTC 2 cut(s) 162, 458
ScrFI CCNGG 4 cut(s) 62, 159, 168, 173
SduI GDGCHC 1 cut(s) 324
SfaNI GCATC 1 cut(s) 493
SfiI GGCCNNNNNGGCC 1 cut(s) 168
Sfr303I CCGCGG 1 cut(s) 388
SgrBI CCGCGG 1 cut(s) 388
SmiMI CAYNNNNRTG 2 cut(s) 204, 347
SmlI CTYRAG 2 cut(s) 128, 323
SmoI CTYRAG 2 cut(s) 128, 323
SnaBI TACGTA 1 cut(s) 451
SsiI CCGC 5 cut(s) 186, 283, 382, 385, 387
SspMI CTAG 1 cut(s) 526
StyD4I CCNGG 4 cut(s) 60, 157, 166, 171
StyI CCWWGG 1 cut(s) 480
TaiI ACGT 4 cut(s) 44, 196, 227, 453
TaqI TCGA 2 cut(s) 270, 462
TauI GCSGC 2 cut(s) 384, 387
TseFI GTSAC 1 cut(s) 194
TseI GCWGC 1 cut(s) 65
Tsp45I GTSAC 1 cut(s) 194
XcmI CCANNNNNNNNNTGG 2 cut(s) 142, 349
XspI CTAG 1 cut(s) 526
ZraI GACGTC 1 cut(s) 225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.