Rorug07G0220700

Lachrymatory-factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0001280
Physical Location & Seq
Forward (+)
19851596 .. 19852981
1386 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0220700.1

Sequence Viewer

Length: 1386 bp
ATGAGTGGTGGGTCTATGCATTCTGGTTTGGTTGGGCTTGTGTGCCTGGCCATTGCCTCTGCTATTTGTTGTTCAAGTGTTGGAAGCTCCAACAACATCATGTGCTTGGAGAGTGAAAGGCATGCTCTTCTCCGGTTCAAACAAGGCCTTGTGGATGACTCCAATGCTCTTGCCTCTTGGAAAAGTGAGAAAGATTGCTGCAAGTGGAGAGGAATAGAATGCAACAACCAAACAGGTCATGTTACCAGTCTTGATTTCTCCTTTAATTATGATCCATATCTCTATTCTGCTGAAGTTCCTTTAAGTGGTGAAATTAGTCCTTCACTACTTGAATTGCCATATCTAAATTACTTGGATCTCAGTTATAATGATTTTGAAGGAACTATGATTCCCAAGTTCATTGGCTCTTTGAGTCAATTGAAAGAACTCAAACTTGCATATGCTAATTTCAATGGACCTGTTCCTCCCCAACTTGGGAACCTCTCTAATTTGCTCACTCTTGATCTTTCCTCCAATGATTTTGAAGGAATGATGATTCCCAAATTCATTGGCTCTATGAGTCAATTGAAAGAGCTCAAACTTGCATATGCTAATTTCAGTGGACCTGTTCCTCCCCAACTTGGAAACCTCTCTGATTTGCACACTCTTGATCTTTCCTTCAATGATTTTGAAGGAATGATGATTCCCAAATTCATTGGCTCTCTGAGTCGATTGAAAGAGCTCAAACTTGTAGAGGCTAATTTCAGTGGACATGTTCCTCCCCAACTTGGAAACCTCTCTAATTTGCACACTCTTGATCTTTACGGTAACGAAGTTGTTAGTTCTGAAAATCTTGAGTGGTTATCTCATCTTTCTTCCTTGAGATACCTGAACATGTCATATCTAGATTTGTCAAAGGTTGTGAATTGGCCACTATCTTTAAGCAAGCTCACTTTACTGACAGAGCTTCAGTTATCCCAGTGTAACCTTCCTGATGTCAATCTAAGATCACTTTCCTTTATTAATTCTTCCACCTCTCTTCAACTGCTTGATCTCTCTCTGAACTCTCTTATTAATTCTTCAATATTTTATTGGATAGCCAATGTCAGCAGCAATTTTGTCCATATTGATCTCCATGACAACAATCTTGAAGGTCCCATCCCAGATGTCTTCACAAGCATGCTTTCTCTAGTAACACTAGATCTGTCATGGAATCAACTTGAAGGTGGGATACCAAAAGGCTTTCAAAACTTATGCAGCTTAGAGTCGTTGACCTTATATCACAACCAATTGTCTGAAAACATTGAGGACTCTATTAAAAACTTATCTTGTGCTGAGAACACACTTGACACCTTGGACTTGAGTGATAACCAGTTTTGCCAGATTTCACACGTTTCTCAAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Polyketide_cyc2

Protein Analysis

461

Amino Acids

50.95

Weight (kDa)

4.64

Isoelectric Point (pI)

36.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 37 - 75 3.8e-13 Leucine rich repeat N-terminal domain
LRR_14 PF23598 104 - 336 7.6e-19 Leucine-rich repeat region
LRR_8 PF13855 368 - 424 5e-08 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 366
AclWI GGATC 2 cut(s) 266, 363
AcoI YGGCCR 2 cut(s) 48, 908
AcsI RAATTY 2 cut(s) 542, 689
AcuI CTGAAG 2 cut(s) 312, 932
AfiI CCNNNNNNNGG 5 cut(s) 305, 473, 474, 620, 767
AflIII ACRYGT 3 cut(s) 751, 873, 1369
AjnI CCWGG 1 cut(s) 45
AloI GAACNNNNNNTCC 2 cut(s) 373, 405
AluBI AGCT 6 cut(s) 87, 574, 721, 928, 946, 1239
AluI AGCT 6 cut(s) 87, 574, 721, 928, 946, 1239
Alw21I GWGCWC 2 cut(s) 576, 723
AlwI GGATC 2 cut(s) 266, 363
AoxI GGCC 3 cut(s) 48, 145, 908
ApeKI GCWGC 3 cut(s) 198, 1089, 1236
ApoI RAATTY 2 cut(s) 542, 689
AseI ATTAAT 2 cut(s) 1002, 1053
AspS9I GGNCC 3 cut(s) 455, 602, 1133
AsuHPI GGTGA 1 cut(s) 320
AvaII GGWCC 3 cut(s) 455, 602, 1133
BalI TGGCCA 2 cut(s) 50, 910
BanII GRGCYC 2 cut(s) 576, 723
BarI GAAGNNNNNNTAC 2 cut(s) 1194, 1226
BbsI GAAGAC 1 cut(s) 1141
Bbv12I GWGCWC 2 cut(s) 576, 723
BbvI GCAGC 3 cut(s) 185, 1101, 1248
BccI CCATC 1 cut(s) 1145
BciT130I CCWGG 1 cut(s) 47
BciVI GTATCC 1 cut(s) 1203
BfaI CTAG 3 cut(s) 884, 1169, 1178
BfuI GTATCC 1 cut(s) 1203
BglII AGATCT 1 cut(s) 1180
BisI GCNGC 3 cut(s) 199, 1090, 1237
BlsI GCNGC 3 cut(s) 200, 1091, 1238
Bme1390I CCNGG 1 cut(s) 47
Bme18I GGWCC 3 cut(s) 455, 602, 1133
BmgT120I GGNCC 3 cut(s) 455, 602, 1133
BmiI GGNNCC 2 cut(s) 479, 1135
BmrFI CCNGG 1 cut(s) 47
BmrI ACTGGG 1 cut(s) 952
BmuI ACTGGG 1 cut(s) 952
BpiI GAAGAC 1 cut(s) 1141
BpuEI CTTGAG 3 cut(s) 854, 880, 1360
BsaBI GATNNNNATC 2 cut(s) 276, 978
BsaJI CCNNGG 1 cut(s) 1332
BsaWI WCCGGW 1 cut(s) 132
Bsc4I CCNNNNNNNGG 5 cut(s) 305, 473, 474, 620, 767
Bse1I ACTGG 3 cut(s) 246, 958, 1351
Bse3DI GCAATG 1 cut(s) 51
Bse8I GATNNNNATC 2 cut(s) 276, 978
BseBI CCWGG 1 cut(s) 47
BseDI CCNNGG 1 cut(s) 1332
BseGI GGATG 2 cut(s) 160, 1137
BseJI GATNNNNATC 2 cut(s) 276, 978
BseLI CCNNNNNNNGG 5 cut(s) 305, 473, 474, 620, 767
BseMI GCAATG 1 cut(s) 51
BseMII CTCAG 3 cut(s) 373, 695, 1305
BseNI ACTGG 3 cut(s) 246, 958, 1351
BseXI GCAGC 3 cut(s) 185, 1101, 1248
BshFI GGCC 3 cut(s) 50, 147, 910
BsiHKAI GWGCWC 2 cut(s) 576, 723
BsiSI CCGG 1 cut(s) 133
BslFI GGGAC 1 cut(s) 1119
BslI CCNNNNNNNGG 5 cut(s) 305, 473, 474, 620, 767
BsmFI GGGAC 1 cut(s) 1119
BsmI GAATGC 2 cut(s) 19, 224
BsnI GGCC 3 cut(s) 50, 147, 910
Bsp1286I GDGCHC 2 cut(s) 576, 723
Bsp143I GATC 9 cut(s) 271, 355, 502, 649, 796, 986, 1030, 1108, 1180
BspANI GGCC 3 cut(s) 50, 147, 910
BspCNI CTCAG 3 cut(s) 372, 696, 1306
BspLI GGNNCC 2 cut(s) 479, 1135
BspPI GGATC 2 cut(s) 266, 363
BspQI GCTCTTC 1 cut(s) 132
BsrDI GCAATG 1 cut(s) 51
BsrI ACTGG 3 cut(s) 246, 958, 1351
BssECI CCNNGG 1 cut(s) 1332
BssMI GATC 9 cut(s) 271, 355, 502, 649, 796, 986, 1030, 1108, 1180
BssT1I CCWWGG 1 cut(s) 1332
Bst2UI CCWGG 1 cut(s) 47
Bst4CI ACNGT 1 cut(s) 806
Bst6I CTCTTC 2 cut(s) 132, 1023
BstC8I GCNNGC 3 cut(s) 123, 926, 1160
BstDEI CTNAG 5 cut(s) 359, 704, 983, 1240, 1314
BstF5I GGATG 2 cut(s) 160, 1137
BstKTI GATC 9 cut(s) 274, 358, 505, 652, 799, 989, 1033, 1111, 1183
BstMBI GATC 9 cut(s) 271, 355, 502, 649, 796, 986, 1030, 1108, 1180
BstNI CCWGG 1 cut(s) 47
BstNSI RCATGY 4 cut(s) 125, 755, 877, 1162
BstSCI CCNGG 1 cut(s) 45
BstV1I GCAGC 3 cut(s) 185, 1101, 1248
BstV2I GAAGAC 1 cut(s) 1141
BstX2I RGATCY 2 cut(s) 355, 1180
BstYI RGATCY 2 cut(s) 355, 1180
BsuI GTATCC 1 cut(s) 1203
BsuRI GGCC 3 cut(s) 50, 147, 910
BtsCI GGATG 2 cut(s) 160, 1137
BtsIMutI CAGTG 3 cut(s) 604, 751, 965
Cac8I GCNNGC 3 cut(s) 123, 926, 1160
Cfr13I GGNCC 3 cut(s) 455, 602, 1133
CviAII CATG 8 cut(s) 100, 122, 239, 752, 874, 1115, 1159, 1188
DdeI CTNAG 5 cut(s) 359, 704, 983, 1240, 1314
DpnI GATC 9 cut(s) 273, 357, 504, 651, 798, 988, 1032, 1110, 1182
DpnII GATC 9 cut(s) 271, 355, 502, 649, 796, 986, 1030, 1108, 1180
EaeI YGGCCR 2 cut(s) 48, 908
Eam1104I CTCTTC 2 cut(s) 132, 1023
EarI CTCTTC 2 cut(s) 132, 1023
Ecl136II GAGCTC 2 cut(s) 574, 721
Eco130I CCWWGG 1 cut(s) 1332
Eco147I AGGCCT 1 cut(s) 147
Eco24I GRGCYC 2 cut(s) 576, 723
Eco47I GGWCC 3 cut(s) 455, 602, 1133
Eco53kI GAGCTC 2 cut(s) 574, 721
Eco57I CTGAAG 2 cut(s) 312, 932
EcoICRI GAGCTC 2 cut(s) 574, 721
EcoO109I RGGNCCY 1 cut(s) 1133
EcoRII CCWGG 1 cut(s) 45
EcoT14I CCWWGG 1 cut(s) 1332
EcoT22I ATGCAT 1 cut(s) 21
EcoT38I GRGCYC 2 cut(s) 576, 723
ErhI CCWWGG 1 cut(s) 1332
FaeI CATG 8 cut(s) 103, 125, 242, 755, 877, 1118, 1162, 1191
FaqI GGGAC 1 cut(s) 1119
FatI CATG 8 cut(s) 99, 121, 238, 751, 873, 1114, 1158, 1187
FauNDI CATATG 2 cut(s) 439, 586
Fnu4HI GCNGC 3 cut(s) 199, 1090, 1237
FokI GGATG 2 cut(s) 167, 1124
FriOI GRGCYC 2 cut(s) 576, 723
Fsp4HI GCNGC 3 cut(s) 199, 1090, 1237
FspBI CTAG 3 cut(s) 884, 1169, 1178
GluI GCNGC 3 cut(s) 199, 1090, 1237
HaeIII GGCC 3 cut(s) 50, 147, 910
HapII CCGG 1 cut(s) 133
Hin1II CATG 8 cut(s) 103, 125, 242, 755, 877, 1118, 1162, 1191
HincII GTYRAC 1 cut(s) 1251
HindII GTYRAC 1 cut(s) 1251
HpaII CCGG 1 cut(s) 133
HphI GGTGA 1 cut(s) 320
Hpy166II GTNNAC 3 cut(s) 602, 749, 1251
Hpy188I TCNGA 5 cut(s) 634, 705, 826, 1041, 1276
Hpy188III TCNNGA 8 cut(s) 251, 500, 647, 794, 833, 884, 971, 1127
Hpy8I GTNNAC 3 cut(s) 602, 749, 1251
HpyAV CCTTC 8 cut(s) 330, 371, 518, 665, 667, 977, 1124, 1196
HpyCH4III ACNGT 1 cut(s) 806
HpyCH4IV ACGT 1 cut(s) 1371
HpyCH4V TGCA 8 cut(s) 19, 201, 222, 437, 584, 640, 787, 1236
HpyF3I CTNAG 5 cut(s) 359, 704, 983, 1240, 1314
HpySE526I ACGT 1 cut(s) 1371
Hsp92II CATG 8 cut(s) 103, 125, 242, 755, 877, 1118, 1162, 1191
Kzo9I GATC 9 cut(s) 271, 355, 502, 649, 796, 986, 1030, 1108, 1180
LguI GCTCTTC 1 cut(s) 132
LmnI GCTCC 1 cut(s) 92
Lsp1109I GCAGC 3 cut(s) 185, 1101, 1248
MaeI CTAG 3 cut(s) 884, 1169, 1178
MaeII ACGT 1 cut(s) 1371
MaeIII GTNAC 4 cut(s) 241, 806, 962, 1171
MalI GATC 9 cut(s) 273, 357, 504, 651, 798, 988, 1032, 1110, 1182
MboI GATC 9 cut(s) 271, 355, 502, 649, 796, 986, 1030, 1108, 1180
MboII GAAGA 6 cut(s) 119, 846, 999, 1010, 1050, 1141
MfeI CAATTG 3 cut(s) 416, 563, 1268
MflI RGATCY 2 cut(s) 355, 1180
MhlI GDGCHC 2 cut(s) 576, 723
MlsI TGGCCA 2 cut(s) 50, 910
MluNI TGGCCA 2 cut(s) 50, 910
MlyI GAGTC 6 cut(s) 152, 421, 568, 715, 1253, 1283
MmeI TCCRAC 2 cut(s) 61, 114
Mox20I TGGCCA 2 cut(s) 50, 910
Mph1103I ATGCAT 1 cut(s) 21
MscI TGGCCA 2 cut(s) 50, 910
MseI TTAA 7 cut(s) 264, 302, 920, 1002, 1053, 1296, 1384
MslI CAYNNNNRTG 1 cut(s) 1157
Msp20I TGGCCA 2 cut(s) 50, 910
MspI CCGG 1 cut(s) 133
MspR9I CCNGG 1 cut(s) 47
MunI CAATTG 3 cut(s) 416, 563, 1268
Mva1269I GAATGC 2 cut(s) 19, 224
MvaI CCWGG 1 cut(s) 47
NdeI CATATG 2 cut(s) 439, 586
NdeII GATC 9 cut(s) 271, 355, 502, 649, 796, 986, 1030, 1108, 1180
NlaIII CATG 8 cut(s) 103, 125, 242, 755, 877, 1118, 1162, 1191
NlaIV GGNNCC 2 cut(s) 479, 1135
NsiI ATGCAT 1 cut(s) 21
NspI RCATGY 4 cut(s) 125, 755, 877, 1162
PaeI GCATGC 2 cut(s) 125, 1162
PceI AGGCCT 1 cut(s) 147
PciI ACATGT 2 cut(s) 751, 873
PciSI GCTCTTC 1 cut(s) 132
PctI GAATGC 2 cut(s) 19, 224
PfeI GAWTC 4 cut(s) 388, 535, 682, 1192
PkrI GCNGC 3 cut(s) 200, 1091, 1238
PleI GAGTC 6 cut(s) 152, 420, 567, 714, 1252, 1283
PpsI GAGTC 6 cut(s) 152, 420, 567, 714, 1252, 1283
PpuMI RGGWCCY 1 cut(s) 1133
PscI ACATGT 2 cut(s) 751, 873
PshBI ATTAAT 2 cut(s) 1002, 1053
PsiI TTATAA 1 cut(s) 366
Psp124BI GAGCTC 2 cut(s) 576, 723
Psp5II RGGWCCY 1 cut(s) 1133
Psp6I CCWGG 1 cut(s) 45
PspGI CCWGG 1 cut(s) 45
PspN4I GGNNCC 2 cut(s) 479, 1135
PspPI GGNCC 3 cut(s) 455, 602, 1133
PspPPI RGGWCCY 1 cut(s) 1133
PsuI RGATCY 2 cut(s) 355, 1180
RseI CAYNNNNRTG 1 cut(s) 1157
SacI GAGCTC 2 cut(s) 576, 723
SapI GCTCTTC 1 cut(s) 132
SaqAI TTAA 7 cut(s) 264, 302, 920, 1002, 1053, 1296, 1384
SatI GCNGC 3 cut(s) 199, 1090, 1237
Sau3AI GATC 9 cut(s) 271, 355, 502, 649, 796, 986, 1030, 1108, 1180
Sau96I GGNCC 3 cut(s) 455, 602, 1133
SchI GAGTC 6 cut(s) 152, 421, 568, 715, 1253, 1283
ScrFI CCNGG 1 cut(s) 47
SduI GDGCHC 2 cut(s) 576, 723
SinI GGWCC 3 cut(s) 455, 602, 1133
SmiMI CAYNNNNRTG 1 cut(s) 1157
SmlI CTYRAG 3 cut(s) 833, 859, 1339
SmoI CTYRAG 3 cut(s) 833, 859, 1339
SphI GCATGC 2 cut(s) 125, 1162
SseBI AGGCCT 1 cut(s) 147
SspI AATATT 1 cut(s) 1065
SspMI CTAG 3 cut(s) 884, 1169, 1178
SstI GAGCTC 2 cut(s) 576, 723
StuI AGGCCT 1 cut(s) 147
StyD4I CCNGG 1 cut(s) 45
StyI CCWWGG 1 cut(s) 1332
TaaI ACNGT 1 cut(s) 806
TaiI ACGT 1 cut(s) 1374
TaqI TCGA 1 cut(s) 709
TfiI GAWTC 4 cut(s) 388, 535, 682, 1192
Tru1I TTAA 7 cut(s) 264, 302, 920, 1002, 1053, 1296, 1384
Tru9I TTAA 7 cut(s) 264, 302, 920, 1002, 1053, 1296, 1384
TscAI CASTG 3 cut(s) 604, 751, 965
TseI GCWGC 3 cut(s) 198, 1089, 1236
TspDTI ATGAA 3 cut(s) 388, 535, 682
TspRI CASTG 3 cut(s) 604, 751, 965
VpaK11BI GGWCC 3 cut(s) 455, 602, 1133
VspI ATTAAT 2 cut(s) 1002, 1053
XapI RAATTY 2 cut(s) 542, 689
XbaI TCTAGA 1 cut(s) 883
XceI RCATGY 4 cut(s) 125, 755, 877, 1162
XspI CTAG 3 cut(s) 884, 1169, 1178
Zsp2I ATGCAT 1 cut(s) 21
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.