Rorug06G0349800

Lachrymatory-factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0001280
Physical Location & Seq
Reverse (-)
49938153 .. 49939358
1206 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0349800.1

Sequence Viewer

Length: 1206 bp
ATGGAGGATCATATTGCTAGAGAAGAGAAATTTGAAAAGAAAGCAAACAAGAAGCTTCATGGATGGAGAGTTTTTGGTTCCAAACGCGATGATATTGCCGAACTGTTGGATCATGCTGCCACCTCTTACAAACTCCCCAATGCCTGGGATAAAGCGAAAGAAATGTACATCAAGTTGGCAAAGCATCATTTGAAGACCAAAACCGAACATGAAGCCGCCGCCACAGCTTTTGTTGATGCTGCACATTGCTGCGACATAACATCTGTTGATGAGGCCATTTCTTGTCTAGATCAGGGAGTCGATTACCTTCTCGATACCGGAAAGTTCAATGCAGCTGCAACATGTTCTGACAAAGCTGCACGCTTGTATAAGGAAATTGCGGAACTATTTGAATCCCAGGAGAATATTGAGAAGGCTATTGAGTTCTTCGAAAAGGCATCTGAGCTCCCTTTAAATGAAGAATTATCAAGTTCCGTGAACTACCAGTGCTATGAGAAAGCCGCTCAACTTGCTGCTCAGAATGAGCAATATCCCAAGGCCATTGAAATCTACGAAAATATGGCGACTTACTCGCTCAACCACAGCTTGCTCAAGCACAAAGTTAAGGGGCATCTTTTCAAGGCCGGAATATGCAGACTCTGCAGAGGAGATGACGCTGCAGTTGCAATGGCCAAGCAATACGCAGATTCATATCCAAGTTTTCGTGATACATGGCAGTGCAAATCCCTGGAGGATATAGCAGTTTCAGTTGATAAACGTGACCTGACCACGTTCCGTGATGTGGTGAAGAAATTCGAAAGTATGACACCACCGGATTCATGGACAACCAACTTGCTGTTGAGAGTGGAGGAGAAGCTGAAAGCTTCGAAGTCCGTGAGAACGCGACGGCCAACCAAGGCGTTAGACTTGAAACCCAGCTCCACCACTACATTTGGTGATAGAGGGAGGCAAAGGAAGATGTGCACCGGCTTGGAAAGATCCCGGACGACTATAGGGGGCACGTCAACGGCAACAGGAAGTAAACTCTTGCGGTCTAGCTTAAGGATCAAAACCGGTGTTGATACAAGCAAGGAGGAGATCAGGAATATGGGAGAGATGCCTCTGACGAATTGGCCGTTCCTCAGAGAGAACTTCAGACGAGCCTTCTTAGTGGCCGGCCAAATGTCCTCCATAAGGGACTTACCTTCCAAAGAAGCTTGCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Polyketide_cyc2

Protein Analysis

401

Amino Acids

45.34

Weight (kDa)

8.63

Isoelectric Point (pI)

41.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SNAP PF14938 7 - 286 4.7e-70 Soluble NSF attachment protein, SNAP
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 144
AccBSI CCGCTC 1 cut(s) 503
AccII CGCG 2 cut(s) 87, 883
AciI CCGC 5 cut(s) 216, 219, 380, 501, 1030
AclWI GGATC 4 cut(s) 15, 117, 972, 1052
AcoI YGGCCR 5 cut(s) 669, 887, 1112, 1152, 1156
AcsI RAATTY 2 cut(s) 29, 791
AcuI CTGAAG 1 cut(s) 1117
AfaI GTAC 1 cut(s) 167
AfiI CCNNNNNNNGG 3 cut(s) 144, 781, 1173
AflII CTTAAG 1 cut(s) 1039
AflIII ACRYGT 1 cut(s) 341
AgeI ACCGGT 1 cut(s) 1052
AgsI TTSAA 7 cut(s) 35, 193, 328, 392, 545, 619, 910
AjiI CACGTC 1 cut(s) 1002
AjnI CCWGG 3 cut(s) 143, 396, 726
Alw21I GWGCWC 2 cut(s) 447, 965
Alw44I GTGCAC 1 cut(s) 961
AlwI GGATC 4 cut(s) 15, 117, 972, 1052
AlwNI CAGNNNCTG 1 cut(s) 639
AoxI GGCC 8 cut(s) 273, 537, 621, 669, 887, 1112, 1152, 1156
ApaLI GTGCAC 1 cut(s) 961
ApeKI GCWGC 8 cut(s) 116, 239, 249, 332, 335, 356, 512, 656
ApoI RAATTY 2 cut(s) 29, 791
AsiGI ACCGGT 1 cut(s) 1052
Asp700I GAANNNNTTC 1 cut(s) 791
AsuC2I CCSGG 1 cut(s) 982
AsuHPI GGTGA 2 cut(s) 796, 947
AsuII TTCGAA 3 cut(s) 429, 795, 866
BaeGI GKGCMC 2 cut(s) 965, 1001
BalI TGGCCA 1 cut(s) 671
BanII GRGCYC 1 cut(s) 447
BbsI GAAGAC 1 cut(s) 200
Bbv12I GWGCWC 2 cut(s) 447, 965
BbvI GCAGC 8 cut(s) 103, 226, 236, 322, 343, 344, 499, 643
BccI CCATC 1 cut(s) 57
BceAI ACGGC 3 cut(s) 902, 1023, 1099
BcgI CGANNNNNNTGC 2 cut(s) 77, 111
BciT130I CCWGG 3 cut(s) 145, 398, 728
BcnI CCSGG 1 cut(s) 982
BfaI CTAG 3 cut(s) 18, 287, 1035
BfmI CTRYAG 3 cut(s) 640, 657, 990
BfrI CTTAAG 1 cut(s) 1039
Bme1390I CCNGG 4 cut(s) 145, 398, 728, 982
BmgBI CACGTC 1 cut(s) 1002
BmiI GGNNCC 1 cut(s) 79
BmrFI CCNGG 4 cut(s) 145, 398, 728, 982
BmsI GCATC 5 cut(s) 193, 226, 446, 619, 1086
BpiI GAAGAC 1 cut(s) 200
BpmI CTGGAG 1 cut(s) 749
Bpu14I TTCGAA 3 cut(s) 429, 795, 866
BpuEI CTTGAG 1 cut(s) 575
BpuMI CCSGG 1 cut(s) 982
BsaBI GATNNNNATC 1 cut(s) 690
BsaJI CCNNGG 5 cut(s) 144, 396, 534, 726, 894
BsaWI WCCGGW 3 cut(s) 317, 811, 1052
Bsc4I CCNNNNNNNGG 3 cut(s) 144, 781, 1173
Bse118I RCCGGY 3 cut(s) 965, 1052, 1154
Bse1I ACTGG 1 cut(s) 484
Bse3DI GCAATG 2 cut(s) 244, 672
Bse8I GATNNNNATC 1 cut(s) 690
BseBI CCWGG 3 cut(s) 145, 398, 728
BseDI CCNNGG 5 cut(s) 144, 396, 534, 726, 894
BseGI GGATG 1 cut(s) 68
BseJI GATNNNNATC 1 cut(s) 690
BseLI CCNNNNNNNGG 3 cut(s) 144, 781, 1173
BseMI GCAATG 2 cut(s) 244, 672
BseMII CTCAG 3 cut(s) 432, 530, 1135
BseNI ACTGG 1 cut(s) 484
BseRI GAGGAG 3 cut(s) 660, 863, 1088
BseSI GKGCMC 2 cut(s) 965, 1001
BseXI GCAGC 8 cut(s) 103, 226, 236, 322, 343, 344, 499, 643
BseYI CCCAGC 1 cut(s) 914
BsgI GTGCAG 2 cut(s) 225, 342
Bsh1236I CGCG 2 cut(s) 87, 883
BshFI GGCC 8 cut(s) 275, 539, 623, 671, 889, 1114, 1154, 1158
BshTI ACCGGT 1 cut(s) 1052
BsiHKAI GWGCWC 2 cut(s) 447, 965
BsiSI CCGG 7 cut(s) 318, 624, 812, 966, 982, 1053, 1155
BslFI GGGAC 1 cut(s) 1190
BslI CCNNNNNNNGG 3 cut(s) 144, 781, 1173
BsmFI GGGAC 1 cut(s) 1190
BsnI GGCC 8 cut(s) 275, 539, 623, 671, 889, 1114, 1154, 1158
Bsp119I TTCGAA 3 cut(s) 429, 795, 866
Bsp1286I GDGCHC 3 cut(s) 447, 965, 1001
Bsp1407I TGTACA 1 cut(s) 165
Bsp143I GATC 6 cut(s) 7, 109, 289, 977, 1044, 1077
BspACI CCGC 5 cut(s) 216, 219, 380, 501, 1030
BspANI GGCC 8 cut(s) 275, 539, 623, 671, 889, 1114, 1154, 1158
BspCNI CTCAG 3 cut(s) 433, 529, 1134
BspFNI CGCG 2 cut(s) 87, 883
BspLI GGNNCC 1 cut(s) 79
BspMAI CTGCAG 2 cut(s) 644, 661
BspPI GGATC 4 cut(s) 15, 117, 972, 1052
BspT104I TTCGAA 3 cut(s) 429, 795, 866
BspTI CTTAAG 1 cut(s) 1039
BsrBI CCGCTC 1 cut(s) 503
BsrDI GCAATG 2 cut(s) 244, 672
BsrFI RCCGGY 3 cut(s) 965, 1052, 1154
BsrGI TGTACA 1 cut(s) 165
BsrI ACTGG 1 cut(s) 484
BssAI RCCGGY 3 cut(s) 965, 1052, 1154
BssECI CCNNGG 5 cut(s) 144, 396, 534, 726, 894
BssMI GATC 6 cut(s) 7, 109, 289, 977, 1044, 1077
BssT1I CCWWGG 2 cut(s) 534, 894
Bst2UI CCWGG 3 cut(s) 145, 398, 728
Bst4CI ACNGT 1 cut(s) 105
Bst6I CTCTTC 1 cut(s) 18
BstAFI CTTAAG 1 cut(s) 1039
BstAPI GCANNNNNTGC 1 cut(s) 639
BstAUI TGTACA 1 cut(s) 165
BstBI TTCGAA 3 cut(s) 429, 795, 866
BstC8I GCNNGC 4 cut(s) 361, 587, 1156, 1198
BstDEI CTNAG 4 cut(s) 441, 516, 1121, 1147
BstENI CCTNNNNNAGG 1 cut(s) 1171
BstF5I GGATG 1 cut(s) 68
BstFNI CGCG 2 cut(s) 87, 883
BstKTI GATC 6 cut(s) 10, 112, 292, 980, 1047, 1080
BstMBI GATC 6 cut(s) 7, 109, 289, 977, 1044, 1077
BstMWI GCNNNNNNNGC 4 cut(s) 224, 509, 639, 662
BstNI CCWGG 3 cut(s) 145, 398, 728
BstNSI RCATGY 2 cut(s) 345, 1204
BstSCI CCNGG 4 cut(s) 143, 396, 726, 980
BstSFI CTRYAG 3 cut(s) 640, 657, 990
BstSLI GKGCMC 2 cut(s) 965, 1001
BstUI CGCG 2 cut(s) 87, 883
BstV1I GCAGC 8 cut(s) 103, 226, 236, 322, 343, 344, 499, 643
BstV2I GAAGAC 1 cut(s) 200
BstX2I RGATCY 1 cut(s) 977
BstYI RGATCY 1 cut(s) 977
BsuRI GGCC 8 cut(s) 275, 539, 623, 671, 889, 1114, 1154, 1158
BtgZI GCGATG 1 cut(s) 102
BtrI CACGTC 1 cut(s) 1002
BtsCI GGATG 1 cut(s) 68
BtsI GCAGTG 1 cut(s) 722
BtsIMutI CAGTG 2 cut(s) 491, 722
Cac8I GCNNGC 4 cut(s) 361, 587, 1156, 1198
CaiI CAGNNNCTG 1 cut(s) 639
Cfr10I RCCGGY 3 cut(s) 965, 1052, 1154
CseI GACGC 1 cut(s) 662
Csp6I GTAC 1 cut(s) 166
CspAI ACCGGT 1 cut(s) 1052
CspCI CAANNNNNGTGG 4 cut(s) 211, 246, 913, 948
CviAII CATG 7 cut(s) 59, 113, 209, 342, 711, 819, 1201
CviQI GTAC 1 cut(s) 166
DdeI CTNAG 4 cut(s) 441, 516, 1121, 1147
DpnI GATC 6 cut(s) 9, 111, 291, 979, 1046, 1079
DpnII GATC 6 cut(s) 7, 109, 289, 977, 1044, 1077
DraI TTTAAA 1 cut(s) 453
EaeI YGGCCR 5 cut(s) 669, 887, 1112, 1152, 1156
Eam1104I CTCTTC 1 cut(s) 18
EarI CTCTTC 1 cut(s) 18
Ecl136II GAGCTC 1 cut(s) 445
Eco130I CCWWGG 2 cut(s) 534, 894
Eco24I GRGCYC 1 cut(s) 447
Eco53kI GAGCTC 1 cut(s) 445
Eco57I CTGAAG 1 cut(s) 1117
EcoICRI GAGCTC 1 cut(s) 445
EcoNI CCTNNNNNAGG 1 cut(s) 1171
EcoRII CCWGG 3 cut(s) 143, 396, 726
EcoT14I CCWWGG 2 cut(s) 534, 894
EcoT38I GRGCYC 1 cut(s) 447
ErhI CCWWGG 2 cut(s) 534, 894
FaeI CATG 7 cut(s) 62, 116, 212, 345, 714, 822, 1204
FaqI GGGAC 1 cut(s) 1190
FatI CATG 7 cut(s) 58, 112, 208, 341, 710, 818, 1200
FokI GGATG 1 cut(s) 75
FriOI GRGCYC 1 cut(s) 447
FseI GGCCGGCC 1 cut(s) 1158
FspBI CTAG 3 cut(s) 18, 287, 1035
GsaI CCCAGC 1 cut(s) 918
GsuI CTGGAG 1 cut(s) 749
HaeIII GGCC 8 cut(s) 275, 539, 623, 671, 889, 1114, 1154, 1158
HapII CCGG 7 cut(s) 318, 624, 812, 966, 982, 1053, 1155
HgaI GACGC 1 cut(s) 662
Hin1II CATG 7 cut(s) 62, 116, 212, 345, 714, 822, 1204
HincII GTYRAC 1 cut(s) 1005
HindII GTYRAC 1 cut(s) 1005
HindIII AAGCTT 3 cut(s) 53, 861, 1194
HinfI GANTC 5 cut(s) 297, 392, 636, 686, 815
HpaII CCGG 7 cut(s) 318, 624, 812, 966, 982, 1053, 1155
HphI GGTGA 2 cut(s) 796, 947
Hpy166II GTNNAC 4 cut(s) 478, 963, 1005, 1022
Hpy188I TCNGA 6 cut(s) 349, 442, 519, 1104, 1124, 1136
Hpy188III TCNNGA 4 cut(s) 287, 311, 704, 1081
Hpy8I GTNNAC 4 cut(s) 478, 963, 1005, 1022
Hpy99I CGWCG 1 cut(s) 888
HpyAV CCTTC 4 cut(s) 317, 406, 1153, 1194
HpyCH4III ACNGT 1 cut(s) 105
HpyCH4IV ACGT 3 cut(s) 757, 770, 1001
HpyF10VI GCNNNNNNNGC 4 cut(s) 224, 509, 639, 662
HpyF3I CTNAG 4 cut(s) 441, 516, 1121, 1147
HpySE526I ACGT 3 cut(s) 757, 770, 1001
Hsp92II CATG 7 cut(s) 62, 116, 212, 345, 714, 822, 1204
KroI GCCGGC 1 cut(s) 1154
KroNI GCCGGC 1 cut(s) 1156
Kzo9I GATC 6 cut(s) 7, 109, 289, 977, 1044, 1077
LmnI GCTCC 2 cut(s) 450, 923
Lsp1109I GCAGC 8 cut(s) 103, 226, 236, 322, 343, 344, 499, 643
LweI GCATC 5 cut(s) 193, 226, 446, 619, 1086
MaeI CTAG 3 cut(s) 18, 287, 1035
MaeII ACGT 3 cut(s) 757, 770, 1001
MaeIII GTNAC 1 cut(s) 758
MalI GATC 6 cut(s) 9, 111, 291, 979, 1046, 1079
MbiI CCGCTC 1 cut(s) 503
MboI GATC 6 cut(s) 7, 109, 289, 977, 1044, 1077
MboII GAAGA 6 cut(s) 35, 205, 418, 470, 799, 967
MflI RGATCY 1 cut(s) 977
MhlI GDGCHC 3 cut(s) 447, 965, 1001
MlsI TGGCCA 1 cut(s) 671
MluCI AATT 5 cut(s) 29, 375, 461, 791, 1108
MluNI TGGCCA 1 cut(s) 671
MlyI GAGTC 2 cut(s) 306, 630
MmeI TCCRAC 1 cut(s) 87
Mox20I TGGCCA 1 cut(s) 671
MroNI GCCGGC 1 cut(s) 1154
MroXI GAANNNNTTC 1 cut(s) 791
MscI TGGCCA 1 cut(s) 671
MseI TTAA 3 cut(s) 452, 603, 1040
MslI CAYNNNNRTG 1 cut(s) 715
Msp20I TGGCCA 1 cut(s) 671
MspA1I CMGCKG 1 cut(s) 335
MspCI CTTAAG 1 cut(s) 1039
MspI CCGG 7 cut(s) 318, 624, 812, 966, 982, 1053, 1155
MspR9I CCNGG 4 cut(s) 145, 398, 728, 982
MvaI CCWGG 3 cut(s) 145, 398, 728
MvnI CGCG 2 cut(s) 87, 883
MwoI GCNNNNNNNGC 4 cut(s) 224, 509, 639, 662
NaeI GCCGGC 1 cut(s) 1156
NciI CCSGG 1 cut(s) 982
NdeII GATC 6 cut(s) 7, 109, 289, 977, 1044, 1077
NgoMIV GCCGGC 1 cut(s) 1154
NlaIII CATG 7 cut(s) 62, 116, 212, 345, 714, 822, 1204
NlaIV GGNNCC 1 cut(s) 79
NmuCI GTSAC 1 cut(s) 758
NspI RCATGY 2 cut(s) 345, 1204
NspV TTCGAA 3 cut(s) 429, 795, 866
PciI ACATGT 1 cut(s) 341
PcsI WCGNNNNNNNCGW 1 cut(s) 1112
PdiI GCCGGC 1 cut(s) 1156
PdmI GAANNNNTTC 1 cut(s) 791
PfeI GAWTC 3 cut(s) 392, 686, 815
PflMI CCANNNNNTGG 1 cut(s) 144
PfoI TCCNGGA 1 cut(s) 980
PinAI ACCGGT 1 cut(s) 1052
PleI GAGTC 2 cut(s) 305, 630
PpsI GAGTC 2 cut(s) 305, 630
PscI ACATGT 1 cut(s) 341
Psp124BI GAGCTC 1 cut(s) 447
Psp6I CCWGG 3 cut(s) 143, 396, 726
PspFI CCCAGC 1 cut(s) 914
PspGI CCWGG 3 cut(s) 143, 396, 726
PspN4I GGNNCC 1 cut(s) 79
PstI CTGCAG 2 cut(s) 644, 661
PstNI CAGNNNCTG 1 cut(s) 639
PsuI RGATCY 1 cut(s) 977
PvuII CAGCTG 1 cut(s) 335
RigI GGCCGGCC 1 cut(s) 1158
RsaI GTAC 1 cut(s) 167
RsaNI GTAC 1 cut(s) 166
RseI CAYNNNNRTG 1 cut(s) 715
SacI GAGCTC 1 cut(s) 447
SaqAI TTAA 3 cut(s) 452, 603, 1040
Sau3AI GATC 6 cut(s) 7, 109, 289, 977, 1044, 1077
SchI GAGTC 2 cut(s) 306, 630
ScrFI CCNGG 4 cut(s) 145, 398, 728, 982
SduI GDGCHC 3 cut(s) 447, 965, 1001
SfaNI GCATC 5 cut(s) 193, 226, 446, 619, 1086
SfcI CTRYAG 3 cut(s) 640, 657, 990
SfuI TTCGAA 3 cut(s) 429, 795, 866
SmiMI CAYNNNNRTG 1 cut(s) 715
SmlI CTYRAG 2 cut(s) 590, 1039
SmoI CTYRAG 2 cut(s) 590, 1039
Sse9I AATT 5 cut(s) 29, 375, 461, 791, 1108
SsiI CCGC 5 cut(s) 216, 219, 380, 501, 1030
SspI AATATT 1 cut(s) 406
SspMI CTAG 3 cut(s) 18, 287, 1035
SstI GAGCTC 1 cut(s) 447
StyD4I CCNGG 4 cut(s) 143, 396, 726, 980
StyI CCWWGG 2 cut(s) 534, 894
TaaI ACNGT 1 cut(s) 105
TaiI ACGT 3 cut(s) 760, 773, 1004
TaqI TCGA 5 cut(s) 300, 312, 429, 795, 866
TasI AATT 5 cut(s) 29, 375, 461, 791, 1108
TatI WGTACW 1 cut(s) 165
TauI GCSGC 3 cut(s) 218, 221, 503
TfiI GAWTC 3 cut(s) 392, 686, 815
Tru1I TTAA 3 cut(s) 452, 603, 1040
Tru9I TTAA 3 cut(s) 452, 603, 1040
TscAI CASTG 2 cut(s) 491, 722
TseFI GTSAC 1 cut(s) 758
TseI GCWGC 8 cut(s) 116, 239, 249, 332, 335, 356, 512, 656
Tsp45I GTSAC 1 cut(s) 758
TspDTI ATGAA 5 cut(s) 47, 225, 471, 678, 807
TspGWI ACGGA 3 cut(s) 463, 764, 862
TspRI CASTG 2 cut(s) 491, 722
Van91I CCANNNNNTGG 1 cut(s) 144
Vha464I CTTAAG 1 cut(s) 1039
VneI GTGCAC 1 cut(s) 961
XagI CCTNNNNNAGG 1 cut(s) 1171
XapI RAATTY 2 cut(s) 29, 791
XbaI TCTAGA 1 cut(s) 286
XceI RCATGY 2 cut(s) 345, 1204
XcmI CCANNNNNNNNNTGG 1 cut(s) 816
XmnI GAANNNNTTC 1 cut(s) 791
XspI CTAG 3 cut(s) 18, 287, 1035
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.