Rroxscaffold_3G00235260

Polyketide cyclase / dehydrase and lipid transport

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0001280
Physical Location & Seq
Forward (+)
22150827 .. 22151330
504 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00235260.1

Sequence Viewer

Length: 504 bp
ATGGCATTAGAAGAACCTGAACAGCCGAAATGGGGAGGCCAAACCTCTGCCAAGCTCAAAGGCTCAAAAGCGCAAGAGGTTTGGCCTCTCCTAGCGGACTTCTGCAACATGGACAAGTGGTTCCCTGGCCTCCACACTTGCTACCAAGTTGGCGGAGTTCCTGGCCAACCCCGTCTTGTTCGGTACTGCGAGCGCGGCACGGATGAGACAACTATCCTGTGGGCAAAAGAGAAGCTACTCACGATCGACCCAATCAACCAGTGTATGACCTACGAGATTGTTGACAACAATATGGGTTTCGAGTTGTATGTTGCAACAATGCAAGTAGTCCCTATCAACAACCATGGCTGCAAGATCGAGTGGTCGTTTGTTTGTGATCAGATTGAGGGCACGAGTTTGGAAGATCTCCAATCTTTCGTTGAGTCTTCACTCCAAGGGATAGCAAAGAAGATGGAGCTTGCTCTTCTATCTAATACTTCTACTAACATGGGTGATAAACGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Polyketide_cyc2

Protein Analysis

167

Amino Acids

18.8

Weight (kDa)

4.85

Isoelectric Point (pI)

33.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Polyketide_cyc2 PF10604 24 - 151 1.7e-19 Polyketide cyclase / dehydrase and lipid transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 195
AciI CCGC 3 cut(s) 95, 153, 195
AclI AACGTT 1 cut(s) 499
AcoI YGGCCR 1 cut(s) 163
AfaI GTAC 1 cut(s) 185
AfiI CCNNNNNNNGG 1 cut(s) 32
AjnI CCWGG 2 cut(s) 124, 160
AloI GAACNNNNNNTCC 2 cut(s) 104, 136
AluBI AGCT 3 cut(s) 55, 235, 457
AluI AGCT 3 cut(s) 55, 235, 457
Alw26I GTCTC 1 cut(s) 200
AoxI GGCC 4 cut(s) 37, 83, 127, 163
ApeKI GCWGC 1 cut(s) 348
AspLEI GCGC 2 cut(s) 73, 195
AsuHPI GGTGA 1 cut(s) 503
BaeGI GKGCMC 1 cut(s) 392
BalI TGGCCA 1 cut(s) 165
BauI CACGAG 1 cut(s) 391
BbsI GAAGAC 1 cut(s) 417
BbvI GCAGC 1 cut(s) 335
BccI CCATC 1 cut(s) 445
BciT130I CCWGG 2 cut(s) 126, 162
BclI TGATCA 1 cut(s) 376
BcoDI GTCTC 1 cut(s) 200
BfaI CTAG 1 cut(s) 92
BglII AGATCT 1 cut(s) 403
BisI GCNGC 2 cut(s) 196, 349
BlsI GCNGC 2 cut(s) 197, 350
Bme1390I CCNGG 2 cut(s) 126, 162
BmiI GGNNCC 1 cut(s) 122
BmrFI CCNGG 2 cut(s) 126, 162
BpiI GAAGAC 1 cut(s) 417
BsaJI CCNNGG 3 cut(s) 124, 343, 433
Bsc4I CCNNNNNNNGG 1 cut(s) 32
Bse1I ACTGG 1 cut(s) 259
BseBI CCWGG 2 cut(s) 126, 162
BseDI CCNNGG 3 cut(s) 124, 343, 433
BseGI GGATG 1 cut(s) 208
BseLI CCNNNNNNNGG 1 cut(s) 32
BseNI ACTGG 1 cut(s) 259
BseSI GKGCMC 1 cut(s) 392
BseXI GCAGC 1 cut(s) 335
Bsh1236I CGCG 1 cut(s) 195
Bsh1285I CGRYCG 1 cut(s) 246
BshFI GGCC 4 cut(s) 39, 85, 129, 165
BsiEI CGRYCG 1 cut(s) 246
BslFI GGGAC 1 cut(s) 314
BslI CCNNNNNNNGG 1 cut(s) 32
BsmAI GTCTC 1 cut(s) 200
BsmFI GGGAC 1 cut(s) 314
BsnI GGCC 4 cut(s) 39, 85, 129, 165
Bsp1286I GDGCHC 1 cut(s) 392
Bsp143I GATC 4 cut(s) 243, 354, 376, 403
Bsp19I CCATGG 1 cut(s) 343
BspACI CCGC 3 cut(s) 95, 153, 195
BspANI GGCC 4 cut(s) 39, 85, 129, 165
BspFNI CGCG 1 cut(s) 195
BspLI GGNNCC 1 cut(s) 122
BspQI GCTCTTC 1 cut(s) 468
BsrI ACTGG 1 cut(s) 259
BssECI CCNNGG 3 cut(s) 124, 343, 433
BssMI GATC 4 cut(s) 243, 354, 376, 403
BssSI CACGAG 1 cut(s) 391
BssT1I CCWWGG 2 cut(s) 343, 433
Bst2BI CACGAG 1 cut(s) 391
Bst2UI CCWGG 2 cut(s) 126, 162
Bst6I CTCTTC 1 cut(s) 468
BstC8I GCNNGC 2 cut(s) 191, 459
BstDSI CCRYGG 1 cut(s) 343
BstF5I GGATG 1 cut(s) 208
BstFNI CGCG 1 cut(s) 195
BstHHI GCGC 2 cut(s) 73, 195
BstKTI GATC 4 cut(s) 246, 357, 379, 406
BstMAI GTCTC 1 cut(s) 200
BstMBI GATC 4 cut(s) 243, 354, 376, 403
BstMCI CGRYCG 1 cut(s) 246
BstMWI GCNNNNNNNGC 1 cut(s) 195
BstNI CCWGG 2 cut(s) 126, 162
BstSCI CCNGG 2 cut(s) 124, 160
BstSLI GKGCMC 1 cut(s) 392
BstUI CGCG 1 cut(s) 195
BstV1I GCAGC 1 cut(s) 335
BstV2I GAAGAC 1 cut(s) 417
BstX2I RGATCY 1 cut(s) 403
BstYI RGATCY 1 cut(s) 403
BsuRI GGCC 4 cut(s) 39, 85, 129, 165
BtgI CCRYGG 1 cut(s) 343
BtsCI GGATG 1 cut(s) 208
BtsIMutI CAGTG 1 cut(s) 266
Cac8I GCNNGC 2 cut(s) 191, 459
CfoI GCGC 2 cut(s) 73, 195
Csp6I GTAC 1 cut(s) 184
CviAII CATG 3 cut(s) 109, 344, 487
CviQI GTAC 1 cut(s) 184
DpnI GATC 4 cut(s) 245, 356, 378, 405
DpnII GATC 4 cut(s) 243, 354, 376, 403
EaeI YGGCCR 1 cut(s) 163
Eam1104I CTCTTC 1 cut(s) 468
EarI CTCTTC 1 cut(s) 468
EciI GGCGGA 1 cut(s) 168
Eco130I CCWWGG 2 cut(s) 343, 433
EcoRII CCWGG 2 cut(s) 124, 160
EcoT14I CCWWGG 2 cut(s) 343, 433
ErhI CCWWGG 2 cut(s) 343, 433
FaeI CATG 3 cut(s) 112, 347, 490
FaiI YATR 6 cut(s) 110, 266, 293, 309, 345, 488
FaqI GGGAC 1 cut(s) 314
FatI CATG 3 cut(s) 108, 343, 486
FbaI TGATCA 1 cut(s) 376
Fnu4HI GCNGC 2 cut(s) 196, 349
FokI GGATG 1 cut(s) 215
Fsp4HI GCNGC 2 cut(s) 196, 349
FspBI CTAG 1 cut(s) 92
GlaI GCGC 2 cut(s) 72, 194
GluI GCNGC 2 cut(s) 196, 349
HaeIII GGCC 4 cut(s) 39, 85, 129, 165
HhaI GCGC 2 cut(s) 73, 195
Hin1II CATG 3 cut(s) 112, 347, 490
Hin6I GCGC 2 cut(s) 71, 193
HinP1I GCGC 2 cut(s) 71, 193
HincII GTYRAC 1 cut(s) 283
HindII GTYRAC 1 cut(s) 283
HinfI GANTC 1 cut(s) 422
HphI GGTGA 1 cut(s) 503
Hpy166II GTNNAC 1 cut(s) 283
Hpy188I TCNGA 1 cut(s) 381
Hpy188III TCNNGA 1 cut(s) 241
Hpy8I GTNNAC 1 cut(s) 283
HpyCH4IV ACGT 1 cut(s) 499
HpyCH4V TGCA 4 cut(s) 105, 314, 322, 351
HpyF10VI GCNNNNNNNGC 1 cut(s) 195
HpySE526I ACGT 1 cut(s) 499
Hsp92II CATG 3 cut(s) 112, 347, 490
HspAI GCGC 2 cut(s) 71, 193
Ksp22I TGATCA 1 cut(s) 376
Kzo9I GATC 4 cut(s) 243, 354, 376, 403
LguI GCTCTTC 1 cut(s) 468
LmnI GCTCC 1 cut(s) 454
LpnPI CCDG 7 cut(s) 30, 111, 138, 147, 174, 230, 272
Lsp1109I GCAGC 1 cut(s) 335
MaeI CTAG 1 cut(s) 92
MaeII ACGT 1 cut(s) 499
MalI GATC 4 cut(s) 245, 356, 378, 405
MboI GATC 4 cut(s) 243, 354, 376, 403
MboII GAAGA 5 cut(s) 23, 413, 417, 455, 460
MflI RGATCY 1 cut(s) 403
MhlI GDGCHC 1 cut(s) 392
MlsI TGGCCA 1 cut(s) 165
MluNI TGGCCA 1 cut(s) 165
MlyI GAGTC 1 cut(s) 431
MnlI CCTC 6 cut(s) 29, 55, 70, 96, 140, 379
Mox20I TGGCCA 1 cut(s) 165
MscI TGGCCA 1 cut(s) 165
Msp20I TGGCCA 1 cut(s) 165
MspR9I CCNGG 2 cut(s) 126, 162
MvaI CCWGG 2 cut(s) 126, 162
MvnI CGCG 1 cut(s) 195
MwoI GCNNNNNNNGC 1 cut(s) 195
NcoI CCATGG 1 cut(s) 343
NdeII GATC 4 cut(s) 243, 354, 376, 403
NlaIII CATG 3 cut(s) 112, 347, 490
NlaIV GGNNCC 1 cut(s) 122
PciSI GCTCTTC 1 cut(s) 468
PkrI GCNGC 2 cut(s) 197, 350
Ple19I CGATCG 1 cut(s) 246
PleI GAGTC 1 cut(s) 430
PpsI GAGTC 1 cut(s) 430
Psp1406I AACGTT 1 cut(s) 499
Psp6I CCWGG 2 cut(s) 124, 160
PspGI CCWGG 2 cut(s) 124, 160
PspN4I GGNNCC 1 cut(s) 122
PsuI RGATCY 1 cut(s) 403
PvuI CGATCG 1 cut(s) 246
RsaI GTAC 1 cut(s) 185
RsaNI GTAC 1 cut(s) 184
SapI GCTCTTC 1 cut(s) 468
SatI GCNGC 2 cut(s) 196, 349
Sau3AI GATC 4 cut(s) 243, 354, 376, 403
SchI GAGTC 1 cut(s) 431
ScrFI CCNGG 2 cut(s) 126, 162
SduI GDGCHC 1 cut(s) 392
SetI ASST 8 cut(s) 19, 47, 57, 81, 237, 272, 459, 502
SsiI CCGC 3 cut(s) 95, 153, 195
SspMI CTAG 1 cut(s) 92
StyD4I CCNGG 2 cut(s) 124, 160
StyI CCWWGG 2 cut(s) 343, 433
TaiI ACGT 1 cut(s) 502
TaqI TCGA 3 cut(s) 246, 300, 357
TauI GCSGC 1 cut(s) 198
TscAI CASTG 1 cut(s) 266
TseI GCWGC 1 cut(s) 348
TspGWI ACGGA 1 cut(s) 215
TspRI CASTG 1 cut(s) 266
XspI CTAG 1 cut(s) 92
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.