Rroxscaffold_4G00296890

Lachrymatory-factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001280
Physical Location & Seq
Reverse (-)
16909174 .. 16910007
834 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00296890.1

Sequence Viewer

Length: 387 bp
ATGTGGAGGGGAGTCCCCGGCCAACCTGGCCTGGTTCGCTACTGCGGGCAACGTGACCATGATGATGACGAAGATGATCAGACGTCGGACACGATCAAGTTCTCAGCTAAAGAGAAGCTACTGATGATCGATCCAGTCAGGCGGTGTTTGAGCTACGAGATACTTGACAACAACTGGGGGCTCAAGTCATATGTTGCAACCATGCAAGTGTTGGATCTGGATGGGAATAACGACCAAAGCCACCGCGGGTGCAAGATTGAGTGGTCGTTTGTTTGTGATCCGATTGAGGGATGGAGGTTCGGAGATTACATATCTCTTCTCGACTCTTGTCTGCTATCCATGGCAAAAAAGATGGAGCATGATGCCTTTCCATCTACTACTAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Polyketide_cyc2

Protein Analysis

128

Amino Acids

14.67

Weight (kDa)

4.7

Isoelectric Point (pI)

38.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Polyketide_cyc2 PF10604 36 - 101 3.5e-07 Polyketide cyclase / dehydrase and lipid transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 86
AccII CGCG 1 cut(s) 246
AciI CCGC 4 cut(s) 45, 142, 244, 246
AclWI GGATC 3 cut(s) 125, 222, 272
AcoI YGGCCR 1 cut(s) 19
AcyI GRCGYC 1 cut(s) 83
AfiI CCNNNNNNNGG 1 cut(s) 287
AjnI CCWGG 2 cut(s) 25, 30
AluBI AGCT 4 cut(s) 107, 118, 153, 384
AluI AGCT 4 cut(s) 107, 118, 153, 384
AlwI GGATC 3 cut(s) 125, 222, 272
AoxI GGCC 2 cut(s) 19, 28
AsuC2I CCSGG 1 cut(s) 18
BanII GRGCYC 1 cut(s) 183
BccI CCATC 4 cut(s) 215, 285, 346, 379
BciT130I CCWGG 2 cut(s) 27, 32
BclI TGATCA 1 cut(s) 76
BcnI CCSGG 1 cut(s) 18
BfaI CTAG 2 cut(s) 381, 385
BglI GCCNNNNNGGC 1 cut(s) 27
Bme1390I CCNGG 3 cut(s) 18, 27, 32
BmrFI CCNGG 3 cut(s) 18, 27, 32
BmrI ACTGGG 1 cut(s) 184
BmsI GCATC 1 cut(s) 352
BmuI ACTGGG 1 cut(s) 184
BoxI GACNNNNGTC 1 cut(s) 327
BpuEI CTTGAG 1 cut(s) 167
BpuMI CCSGG 1 cut(s) 18
Bsa29I ATCGAT 1 cut(s) 129
BsaHI GRCGYC 1 cut(s) 83
BsaJI CCNNGG 3 cut(s) 16, 244, 339
Bsc4I CCNNNNNNNGG 1 cut(s) 287
Bse1I ACTGG 2 cut(s) 134, 179
BseBI CCWGG 2 cut(s) 27, 32
BseCI ATCGAT 1 cut(s) 129
BseDI CCNNGG 3 cut(s) 16, 244, 339
BseGI GGATG 2 cut(s) 226, 296
BseLI CCNNNNNNNGG 1 cut(s) 287
BseMII CTCAG 1 cut(s) 117
BseNI ACTGG 2 cut(s) 134, 179
Bsh1236I CGCG 1 cut(s) 246
BshFI GGCC 2 cut(s) 21, 30
BshVI ATCGAT 1 cut(s) 129
BsiSI CCGG 1 cut(s) 18
BslI CCNNNNNNNGG 1 cut(s) 287
BsnI GGCC 2 cut(s) 21, 30
Bsp1286I GDGCHC 1 cut(s) 183
Bsp143I GATC 6 cut(s) 76, 93, 126, 130, 214, 277
Bsp19I CCATGG 1 cut(s) 339
BspACI CCGC 4 cut(s) 45, 142, 244, 246
BspANI GGCC 2 cut(s) 21, 30
BspCNI CTCAG 1 cut(s) 116
BspDI ATCGAT 1 cut(s) 129
BspFNI CGCG 1 cut(s) 246
BspPI GGATC 3 cut(s) 125, 222, 272
BsrI ACTGG 2 cut(s) 134, 179
BssECI CCNNGG 3 cut(s) 16, 244, 339
BssMI GATC 6 cut(s) 76, 93, 126, 130, 214, 277
BssNI GRCGYC 1 cut(s) 83
BssT1I CCWWGG 1 cut(s) 339
Bst2UI CCWGG 2 cut(s) 27, 32
Bst6I CTCTTC 1 cut(s) 321
BstACI GRCGYC 1 cut(s) 83
BstC8I GCNNGC 1 cut(s) 47
BstDEI CTNAG 1 cut(s) 103
BstDSI CCRYGG 2 cut(s) 244, 339
BstF5I GGATG 2 cut(s) 226, 296
BstFNI CGCG 1 cut(s) 246
BstKTI GATC 6 cut(s) 79, 96, 129, 133, 217, 280
BstMBI GATC 6 cut(s) 76, 93, 126, 130, 214, 277
BstMWI GCNNNNNNNGC 2 cut(s) 27, 36
BstNI CCWGG 2 cut(s) 27, 32
BstPAI GACNNNNGTC 1 cut(s) 327
BstSCI CCNGG 3 cut(s) 16, 25, 30
BstUI CGCG 1 cut(s) 246
BstX2I RGATCY 1 cut(s) 214
BstYI RGATCY 1 cut(s) 214
Bsu15I ATCGAT 1 cut(s) 129
BsuRI GGCC 2 cut(s) 21, 30
BsuTUI ATCGAT 1 cut(s) 129
BtgI CCRYGG 2 cut(s) 244, 339
BtsCI GGATG 2 cut(s) 226, 296
Cac8I GCNNGC 1 cut(s) 47
Cfr42I CCGCGG 1 cut(s) 247
ClaI ATCGAT 1 cut(s) 129
CviAII CATG 4 cut(s) 59, 202, 340, 359
CviJI RGCY 8 cut(s) 21, 30, 107, 118, 153, 181, 240, 384
CviKI_1 RGCY 8 cut(s) 21, 30, 107, 118, 153, 181, 240, 384
DdeI CTNAG 1 cut(s) 103
DpnI GATC 6 cut(s) 78, 95, 128, 132, 216, 279
DpnII GATC 6 cut(s) 76, 93, 126, 130, 214, 277
EaeI YGGCCR 1 cut(s) 19
Eam1104I CTCTTC 1 cut(s) 321
EarI CTCTTC 1 cut(s) 321
Eco130I CCWWGG 1 cut(s) 339
Eco24I GRGCYC 1 cut(s) 183
EcoRII CCWGG 2 cut(s) 25, 30
EcoT14I CCWWGG 1 cut(s) 339
EcoT38I GRGCYC 1 cut(s) 183
ErhI CCWWGG 1 cut(s) 339
FaeI CATG 4 cut(s) 62, 205, 343, 362
FaiI YATR 7 cut(s) 60, 190, 192, 203, 311, 341, 360
FatI CATG 4 cut(s) 58, 201, 339, 358
FauI CCCGC 2 cut(s) 38, 239
FauNDI CATATG 1 cut(s) 190
FbaI TGATCA 1 cut(s) 76
FokI GGATG 2 cut(s) 233, 303
FriOI GRGCYC 1 cut(s) 183
FspBI CTAG 2 cut(s) 381, 385
HaeIII GGCC 2 cut(s) 21, 30
HapII CCGG 1 cut(s) 18
Hin1I GRCGYC 1 cut(s) 83
Hin1II CATG 4 cut(s) 62, 205, 343, 362
HinfI GANTC 2 cut(s) 12, 323
HpaII CCGG 1 cut(s) 18
Hpy188I TCNGA 4 cut(s) 81, 88, 282, 302
Hpy188III TCNNGA 2 cut(s) 218, 320
Hpy99I CGWCG 1 cut(s) 88
HpyCH4IV ACGT 2 cut(s) 52, 83
HpyCH4V TGCA 3 cut(s) 197, 205, 252
HpyF10VI GCNNNNNNNGC 2 cut(s) 27, 36
HpyF3I CTNAG 1 cut(s) 103
HpySE526I ACGT 2 cut(s) 52, 83
Hsp92I GRCGYC 1 cut(s) 83
Hsp92II CATG 4 cut(s) 62, 205, 343, 362
Ksp22I TGATCA 1 cut(s) 76
KspI CCGCGG 1 cut(s) 247
Kzo9I GATC 6 cut(s) 76, 93, 126, 130, 214, 277
LmnI GCTCC 1 cut(s) 355
LpnPI CCDG 9 cut(s) 12, 17, 31, 39, 44, 124, 147, 160, 203
LweI GCATC 1 cut(s) 352
MaeI CTAG 2 cut(s) 381, 385
MaeII ACGT 2 cut(s) 52, 83
MaeIII GTNAC 1 cut(s) 53
MalI GATC 6 cut(s) 78, 95, 128, 132, 216, 279
MboI GATC 6 cut(s) 76, 93, 126, 130, 214, 277
MboII GAAGA 2 cut(s) 83, 308
MflI RGATCY 1 cut(s) 214
MhlI GDGCHC 1 cut(s) 183
MlyI GAGTC 2 cut(s) 21, 317
MmeI TCCRAC 2 cut(s) 66, 192
MnlI CCTC 2 cut(s) 280, 288
MslI CAYNNNNRTG 2 cut(s) 63, 206
MspA1I CMGCKG 1 cut(s) 246
MspI CCGG 1 cut(s) 18
MspR9I CCNGG 3 cut(s) 18, 27, 32
MvaI CCWGG 2 cut(s) 27, 32
MvnI CGCG 1 cut(s) 246
MwoI GCNNNNNNNGC 2 cut(s) 27, 36
NciI CCSGG 1 cut(s) 18
NcoI CCATGG 1 cut(s) 339
NdeI CATATG 1 cut(s) 190
NdeII GATC 6 cut(s) 76, 93, 126, 130, 214, 277
NlaIII CATG 4 cut(s) 62, 205, 343, 362
NmuCI GTSAC 1 cut(s) 53
PcsI WCGNNNNNNNCGW 1 cut(s) 89
PleI GAGTC 2 cut(s) 20, 317
PpsI GAGTC 2 cut(s) 20, 317
PshAI GACNNNNGTC 1 cut(s) 327
Psp6I CCWGG 2 cut(s) 25, 30
PspGI CCWGG 2 cut(s) 25, 30
PsuI RGATCY 1 cut(s) 214
RseI CAYNNNNRTG 2 cut(s) 63, 206
SacII CCGCGG 1 cut(s) 247
Sau3AI GATC 6 cut(s) 76, 93, 126, 130, 214, 277
SchI GAGTC 2 cut(s) 21, 317
ScrFI CCNGG 3 cut(s) 18, 27, 32
SduI GDGCHC 1 cut(s) 183
SetI ASST 8 cut(s) 28, 55, 86, 109, 120, 155, 299, 386
SfaNI GCATC 1 cut(s) 352
SfiI GGCCNNNNNGGCC 1 cut(s) 27
Sfr303I CCGCGG 1 cut(s) 247
SgrBI CCGCGG 1 cut(s) 247
SmiMI CAYNNNNRTG 2 cut(s) 63, 206
SmlI CTYRAG 1 cut(s) 182
SmoI CTYRAG 1 cut(s) 182
SsiI CCGC 4 cut(s) 45, 142, 244, 246
SspMI CTAG 2 cut(s) 381, 385
StyD4I CCNGG 3 cut(s) 16, 25, 30
StyI CCWWGG 1 cut(s) 339
TaiI ACGT 2 cut(s) 55, 86
TaqI TCGA 2 cut(s) 129, 321
TseFI GTSAC 1 cut(s) 53
Tsp45I GTSAC 1 cut(s) 53
XcmI CCANNNNNNNNNTGG 1 cut(s) 208
XspI CTAG 2 cut(s) 381, 385
ZraI GACGTC 1 cut(s) 84
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.