RchiOBHm_Chr6g0306491

Lachrymatory-factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0001280
Physical Location & Seq
Forward (+)
65175079 .. 65176020
942 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27551

Sequence Viewer

Length: 507 bp
ATGGCATCAGAGGTTCAAGCAAAGCCGAAATGGGAAGGTAAAACCTCAGCCGAGCTCAAAAGCTCACCAGCACAACAGGTCTGGCCTTTCCTTGCCGATTTCTGCAACTTACACAAAGTCTTCCCAGGCCTTGAAACTTGCTACCAAGTCGAGGGAGTCCCCGGCCAGCCGGGCCTGATCCGCTGCTGCGCCGGACTGGCTAGCAATAACGACGAGAGCACCGTAATGTGGGCCAAAGAGAAGCTGATTGCGGTTGATCCAATCAAACGGTGCTTCAGCTACCAGATGCTTGAGAATAACAAGGGGTACAAGTCGTATGTTGCAACCATGCAAGTGGTTCCGGTGATAAACGACGATGAAGGGTGCATGATTGAGTGGTCCTTTGTTTCTGATCCGGTGGAAGGGTGGGGATTGGAGGAGTTTCAGTCTTATCGTGAGTCGAGTCTACAAGGGATGGCAAAGAAGATTGAGGATGCTCTTCAGCCCAGTACTAGTGCTACCGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Polyketide_cyc2

Protein Analysis

168

Amino Acids

18.65

Weight (kDa)

4.77

Isoelectric Point (pI)

43.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Polyketide_cyc2 PF10604 22 - 157 3.5e-19 Polyketide cyclase / dehydrase and lipid transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 445
AciI CCGC 2 cut(s) 181, 251
AclWI GGATC 3 cut(s) 172, 251, 386
AcoI YGGCCR 1 cut(s) 163
AcuI CTGAAG 2 cut(s) 259, 464
AfaI GTAC 2 cut(s) 308, 490
AfiI CCNNNNNNNGG 3 cut(s) 151, 228, 401
AgsI TTSAA 2 cut(s) 17, 134
AhlI ACTAGT 1 cut(s) 491
AjnI CCWGG 1 cut(s) 124
AluBI AGCT 4 cut(s) 55, 63, 244, 279
AluI AGCT 4 cut(s) 55, 63, 244, 279
Alw21I GWGCWC 2 cut(s) 57, 221
AlwI GGATC 3 cut(s) 172, 251, 386
AoxI GGCC 5 cut(s) 83, 127, 163, 172, 231
ApeKI GCWGC 2 cut(s) 183, 186
AspLEI GCGC 1 cut(s) 191
AspS9I GGNCC 3 cut(s) 172, 231, 378
AsuC2I CCSGG 2 cut(s) 162, 171
AsuHPI GGTGA 2 cut(s) 57, 355
AsuNHI GCTAGC 1 cut(s) 200
AvaII GGWCC 1 cut(s) 378
BanII GRGCYC 1 cut(s) 57
BbsI GAAGAC 1 cut(s) 112
Bbv12I GWGCWC 2 cut(s) 57, 221
BbvCI CCTCAGC 1 cut(s) 46
BbvI GCAGC 2 cut(s) 170, 173
BccI CCATC 1 cut(s) 448
BciT130I CCWGG 1 cut(s) 126
BcnI CCSGG 2 cut(s) 162, 171
BcuI ACTAGT 1 cut(s) 491
BfaI CTAG 2 cut(s) 201, 492
BglI GCCNNNNNGGC 2 cut(s) 171, 197
BisI GCNGC 2 cut(s) 184, 187
BlsI GCNGC 2 cut(s) 185, 188
BmcAI AGTACT 1 cut(s) 490
Bme1390I CCNGG 3 cut(s) 126, 162, 171
Bme18I GGWCC 1 cut(s) 378
BmgT120I GGNCC 3 cut(s) 172, 231, 378
BmiI GGNNCC 1 cut(s) 339
BmrFI CCNGG 3 cut(s) 126, 162, 171
BmrI ACTGGG 1 cut(s) 480
BmsI GCATC 3 cut(s) 14, 276, 463
BmtI GCTAGC 1 cut(s) 204
BmuI ACTGGG 1 cut(s) 480
BpiI GAAGAC 1 cut(s) 112
Bpu10I CCTNAGC 1 cut(s) 46
BpuEI CTTGAG 1 cut(s) 311
BpuMI CCSGG 2 cut(s) 162, 171
BsaJI CCNNGG 2 cut(s) 124, 160
BsaWI WCCGGW 2 cut(s) 340, 394
BsaXI ACNNNNNCTCC 2 cut(s) 410, 440
Bsc4I CCNNNNNNNGG 3 cut(s) 151, 228, 401
Bse1I ACTGG 2 cut(s) 201, 486
BseBI CCWGG 1 cut(s) 126
BseDI CCNNGG 2 cut(s) 124, 160
BseGI GGATG 2 cut(s) 459, 478
BseLI CCNNNNNNNGG 3 cut(s) 151, 228, 401
BseMII CTCAG 1 cut(s) 60
BseNI ACTGG 2 cut(s) 201, 486
BseRI GAGGAG 1 cut(s) 431
BseXI GCAGC 2 cut(s) 170, 173
BshFI GGCC 5 cut(s) 85, 129, 165, 174, 233
BsiHKAI GWGCWC 2 cut(s) 57, 221
BsiSI CCGG 5 cut(s) 162, 170, 192, 341, 395
BslFI GGGAC 1 cut(s) 143
BslI CCNNNNNNNGG 3 cut(s) 151, 228, 401
BsmFI GGGAC 1 cut(s) 143
BsnI GGCC 5 cut(s) 85, 129, 165, 174, 233
Bsp1286I GDGCHC 2 cut(s) 57, 221
Bsp143I GATC 3 cut(s) 177, 256, 391
BspACI CCGC 2 cut(s) 181, 251
BspANI GGCC 5 cut(s) 85, 129, 165, 174, 233
BspCNI CTCAG 1 cut(s) 59
BspLI GGNNCC 1 cut(s) 339
BspOI GCTAGC 1 cut(s) 204
BspPI GGATC 3 cut(s) 172, 251, 386
BspQI GCTCTTC 1 cut(s) 483
BsrI ACTGG 2 cut(s) 201, 486
BssECI CCNNGG 2 cut(s) 124, 160
BssMI GATC 3 cut(s) 177, 256, 391
Bst2UI CCWGG 1 cut(s) 126
Bst4CI ACNGT 3 cut(s) 223, 270, 502
Bst6I CTCTTC 1 cut(s) 483
BstC8I GCNNGC 2 cut(s) 167, 202
BstDEI CTNAG 1 cut(s) 46
BstF5I GGATG 2 cut(s) 459, 478
BstHHI GCGC 1 cut(s) 191
BstKTI GATC 3 cut(s) 180, 259, 394
BstMBI GATC 3 cut(s) 177, 256, 391
BstMWI GCNNNNNNNGC 3 cut(s) 171, 180, 197
BstNI CCWGG 1 cut(s) 126
BstSCI CCNGG 3 cut(s) 124, 160, 169
BstV1I GCAGC 2 cut(s) 170, 173
BstV2I GAAGAC 1 cut(s) 112
BstXI CCANNNNNNTGG 1 cut(s) 334
BsuRI GGCC 5 cut(s) 85, 129, 165, 174, 233
BtsCI GGATG 2 cut(s) 459, 478
Cac8I GCNNGC 2 cut(s) 167, 202
CfoI GCGC 1 cut(s) 191
Cfr13I GGNCC 3 cut(s) 172, 231, 378
Csp6I GTAC 2 cut(s) 307, 489
CviAII CATG 2 cut(s) 328, 367
CviQI GTAC 2 cut(s) 307, 489
DdeI CTNAG 1 cut(s) 46
DpnI GATC 3 cut(s) 179, 258, 393
DpnII GATC 3 cut(s) 177, 256, 391
EaeI YGGCCR 1 cut(s) 163
Eam1104I CTCTTC 1 cut(s) 483
EarI CTCTTC 1 cut(s) 483
Ecl136II GAGCTC 1 cut(s) 55
Eco147I AGGCCT 1 cut(s) 129
Eco24I GRGCYC 1 cut(s) 57
Eco47I GGWCC 1 cut(s) 378
Eco53kI GAGCTC 1 cut(s) 55
Eco57I CTGAAG 2 cut(s) 259, 464
EcoICRI GAGCTC 1 cut(s) 55
EcoRII CCWGG 1 cut(s) 124
EcoT38I GRGCYC 1 cut(s) 57
FaeI CATG 2 cut(s) 331, 370
FaiI YATR 3 cut(s) 318, 329, 368
FaqI GGGAC 1 cut(s) 143
FatI CATG 2 cut(s) 327, 366
FblI GTMKAC 1 cut(s) 445
Fnu4HI GCNGC 2 cut(s) 184, 187
FokI GGATG 2 cut(s) 466, 485
FriOI GRGCYC 1 cut(s) 57
Fsp4HI GCNGC 2 cut(s) 184, 187
FspBI CTAG 2 cut(s) 201, 492
GlaI GCGC 1 cut(s) 190
GluI GCNGC 2 cut(s) 184, 187
HaeIII GGCC 5 cut(s) 85, 129, 165, 174, 233
HapII CCGG 5 cut(s) 162, 170, 192, 341, 395
HhaI GCGC 1 cut(s) 191
Hin1II CATG 2 cut(s) 331, 370
Hin6I GCGC 1 cut(s) 189
HinP1I GCGC 1 cut(s) 189
HinfI GANTC 3 cut(s) 156, 437, 442
HpaII CCGG 5 cut(s) 162, 170, 192, 341, 395
HphI GGTGA 2 cut(s) 57, 355
Hpy166II GTNNAC 1 cut(s) 446
Hpy188I TCNGA 2 cut(s) 10, 391
Hpy188III TCNNGA 1 cut(s) 434
Hpy8I GTNNAC 1 cut(s) 446
Hpy99I CGWCG 2 cut(s) 215, 356
HpyAV CCTTC 3 cut(s) 29, 353, 395
HpyCH4III ACNGT 3 cut(s) 223, 270, 502
HpyCH4V TGCA 4 cut(s) 105, 323, 331, 366
HpyF10VI GCNNNNNNNGC 3 cut(s) 171, 180, 197
HpyF3I CTNAG 1 cut(s) 46
Hsp92II CATG 2 cut(s) 331, 370
HspAI GCGC 1 cut(s) 189
Kzo9I GATC 3 cut(s) 177, 256, 391
LguI GCTCTTC 1 cut(s) 483
Lsp1109I GCAGC 2 cut(s) 170, 173
LweI GCATC 3 cut(s) 14, 276, 463
MaeI CTAG 2 cut(s) 201, 492
MalI GATC 3 cut(s) 179, 258, 393
MboI GATC 3 cut(s) 177, 256, 391
MboII GAAGA 3 cut(s) 112, 470, 475
MhlI GDGCHC 2 cut(s) 57, 221
MlyI GAGTC 3 cut(s) 165, 446, 451
MnlI CCTC 5 cut(s) 4, 55, 145, 409, 463
MslI CAYNNNNRTG 2 cut(s) 224, 332
MspA1I CMGCKG 1 cut(s) 183
MspI CCGG 5 cut(s) 162, 170, 192, 341, 395
MspR9I CCNGG 3 cut(s) 126, 162, 171
MvaI CCWGG 1 cut(s) 126
MwoI GCNNNNNNNGC 3 cut(s) 171, 180, 197
NciI CCSGG 2 cut(s) 162, 171
NdeII GATC 3 cut(s) 177, 256, 391
NheI GCTAGC 1 cut(s) 200
NlaIII CATG 2 cut(s) 331, 370
NlaIV GGNNCC 1 cut(s) 339
NmeAIII GCCGAG 1 cut(s) 76
PceI AGGCCT 1 cut(s) 129
PciSI GCTCTTC 1 cut(s) 483
PcsI WCGNNNNNNNCGW 1 cut(s) 219
PkrI GCNGC 2 cut(s) 185, 188
PleI GAGTC 3 cut(s) 164, 445, 450
PpsI GAGTC 3 cut(s) 164, 445, 450
Psp124BI GAGCTC 1 cut(s) 57
Psp6I CCWGG 1 cut(s) 124
PspGI CCWGG 1 cut(s) 124
PspN4I GGNNCC 1 cut(s) 339
PspPI GGNCC 3 cut(s) 172, 231, 378
RsaI GTAC 2 cut(s) 308, 490
RsaNI GTAC 2 cut(s) 307, 489
RseI CAYNNNNRTG 2 cut(s) 224, 332
SacI GAGCTC 1 cut(s) 57
SapI GCTCTTC 1 cut(s) 483
SatI GCNGC 2 cut(s) 184, 187
Sau3AI GATC 3 cut(s) 177, 256, 391
Sau96I GGNCC 3 cut(s) 172, 231, 378
ScaI AGTACT 1 cut(s) 490
SchI GAGTC 3 cut(s) 165, 446, 451
ScrFI CCNGG 3 cut(s) 126, 162, 171
SduI GDGCHC 2 cut(s) 57, 221
SetI ASST 8 cut(s) 15, 40, 47, 57, 65, 81, 246, 281
SfaNI GCATC 3 cut(s) 14, 276, 463
SfiI GGCCNNNNNGGCC 1 cut(s) 171
SinI GGWCC 1 cut(s) 378
SmiMI CAYNNNNRTG 2 cut(s) 224, 332
SmlI CTYRAG 1 cut(s) 290
SmoI CTYRAG 1 cut(s) 290
SpeI ACTAGT 1 cut(s) 491
SseBI AGGCCT 1 cut(s) 129
SsiI CCGC 2 cut(s) 181, 251
SspMI CTAG 2 cut(s) 201, 492
SstI GAGCTC 1 cut(s) 57
StuI AGGCCT 1 cut(s) 129
StyD4I CCNGG 3 cut(s) 124, 160, 169
TaaI ACNGT 3 cut(s) 223, 270, 502
TaqI TCGA 2 cut(s) 150, 440
TatI WGTACW 1 cut(s) 488
TseI GCWGC 2 cut(s) 183, 186
TspDTI ATGAA 1 cut(s) 372
VpaK11BI GGWCC 1 cut(s) 378
XmiI GTMKAC 1 cut(s) 445
XspI CTAG 2 cut(s) 201, 492
ZrmI AGTACT 1 cut(s) 490
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.