Rorug06G0349900

Lachrymatory-factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0001280
Physical Location & Seq
Reverse (-)
49941517 .. 49944649
3133 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0349900.1

Sequence Viewer

Length: 813 bp
ATGGATGTTAGAAAGGAAATTCTCAGGCAGGTGTTCGGCGATTCATCGGATAGTGAAGACTTGGAATTGGGCGATGGATCGGAGTCCGACCCGAATCAGAGATGGGTACCAATAGAACAAATCAAAGGGCTGTGGCTGTGCAGAGACTTCCTCTCTCCTCAGAACCAGTCTGCCTTGCTCTCCACAATCCAAAACGAAGGATGGTTCACTGAAGCCTCTCACAATCAGGCTATGAGGTTCGGAGACCTTCCGGATTGGGCAGCTGAGCTTTCGGATTCTATTCACAAGGTTGTGCTTTCATGTGATAGTGATTCTCATCCTATCGAGTTGGGAAGTGCTTGTGATGGGAAGCAGAATGGCAGTCCGTTTCCGTCGGAACTTTTATCCAGAGTGCCACTCTTTGATCAACTTATCCTAAACTCTTACCAACCAGGTGAGGGGATCTGCGCCCACGTTGACCTATTGCGCTTTGAAGATGGAATTGCTATCCTCTCCCTAGAGTCATCATGCGTGATGCATTTTAGTCCAGTTGAAGGAACAAGTGGGGGCTTTCTAGAGGATCAGGGAAAGGATCCAGTGACGACCAAGATTCCTGTTTATCTTACACCAGGATCCCTGATTCTAATGTCAGGAGAAGCACGCTACCAATGGAAGCACGAGATCAACCGCAAACCTGGATTTCAGAAGTGGCAAGGAGAGGAGCTAAATCAGAAGAAGAGAATCTCCATTACTCTGAGGAAGCTTTGCCAGCCTACACAGGTCCTGAATGGAAATGATGGATCAAAGTATTATGTGCTAGCTGACGTTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Polyketide_cyc2

Protein Analysis

270

Amino Acids

30.1

Weight (kDa)

4.77

Isoelectric Point (pI)

55.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy_2 PF13532 44 - 246 1.8e-12 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 19
Acc36I ACCTGC 1 cut(s) 19
Acc65I GGTACC 1 cut(s) 106
AccB1I GGYRCC 1 cut(s) 106
AccIII TCCGGA 1 cut(s) 250
AciI CCGC 1 cut(s) 667
AclWI GGATC 8 cut(s) 85, 449, 566, 567, 579, 606, 619, 787
AcsI RAATTY 1 cut(s) 18
AcuI CTGAAG 1 cut(s) 231
AfaI GTAC 1 cut(s) 108
AfiI CCNNNNNNNGG 2 cut(s) 437, 533
AgsI TTSAA 2 cut(s) 473, 533
AjnI CCWGG 3 cut(s) 430, 607, 673
AluBI AGCT 5 cut(s) 263, 268, 703, 742, 800
AluI AGCT 5 cut(s) 263, 268, 703, 742, 800
Alw26I GTCTC 2 cut(s) 138, 237
AlwI GGATC 8 cut(s) 85, 449, 566, 567, 579, 606, 619, 787
AlwNI CAGNNNCTG 1 cut(s) 763
Aor13HI TCCGGA 1 cut(s) 250
ApeKI GCWGC 1 cut(s) 260
ApoI RAATTY 1 cut(s) 18
Asp718I GGTACC 1 cut(s) 106
AspLEI GCGC 2 cut(s) 449, 468
AspS9I GGNCC 1 cut(s) 760
AsuHPI GGTGA 1 cut(s) 446
AsuNHI GCTAGC 1 cut(s) 796
AvaII GGWCC 1 cut(s) 760
BamHI GGATCC 2 cut(s) 571, 611
BanI GGYRCC 1 cut(s) 106
BauI CACGAG 1 cut(s) 656
BbsI GAAGAC 1 cut(s) 63
BbvI GCAGC 1 cut(s) 272
BccI CCATC 6 cut(s) 68, 96, 195, 338, 470, 770
BciT130I CCWGG 3 cut(s) 432, 609, 675
BclI TGATCA 1 cut(s) 403
BcoDI GTCTC 2 cut(s) 138, 237
BfaI CTAG 3 cut(s) 497, 554, 797
BfuAI ACCTGC 1 cut(s) 19
BisI GCNGC 1 cut(s) 261
BlpI GCTNAGC 1 cut(s) 264
BlsI GCNGC 1 cut(s) 262
Bme1390I CCNGG 3 cut(s) 432, 609, 675
Bme18I GGWCC 1 cut(s) 760
BmgT120I GGNCC 1 cut(s) 760
BmiI GGNNCC 3 cut(s) 108, 573, 613
BmrFI CCNGG 3 cut(s) 432, 609, 675
BmsI GCATC 1 cut(s) 504
BmtI GCTAGC 1 cut(s) 800
BpiI GAAGAC 1 cut(s) 63
BplI GAGNNNNNCTC 4 cut(s) 135, 167, 381, 413
Bpu1102I GCTNAGC 1 cut(s) 264
BsaBI GATNNNNATC 1 cut(s) 315
BsaI GGTCTC 1 cut(s) 237
BsaWI WCCGGW 1 cut(s) 250
Bsc4I CCNNNNNNNGG 2 cut(s) 437, 533
Bse1I ACTGG 3 cut(s) 166, 527, 575
Bse8I GATNNNNATC 1 cut(s) 315
BseAI TCCGGA 1 cut(s) 250
BseBI CCWGG 3 cut(s) 432, 609, 675
BseGI GGATG 3 cut(s) 10, 206, 316
BseJI GATNNNNATC 1 cut(s) 315
BseLI CCNNNNNNNGG 2 cut(s) 437, 533
BseMII CTCAG 4 cut(s) 37, 173, 255, 725
BseNI ACTGG 3 cut(s) 166, 527, 575
BseRI GAGGAG 2 cut(s) 147, 713
BseXI GCAGC 1 cut(s) 272
BsgI GTGCAG 1 cut(s) 160
BshNI GGYRCC 1 cut(s) 106
BsiSI CCGG 1 cut(s) 251
BslI CCNNNNNNNGG 2 cut(s) 437, 533
BsmAI GTCTC 2 cut(s) 138, 237
Bso31I GGTCTC 1 cut(s) 237
Bsp13I TCCGGA 1 cut(s) 250
Bsp143I GATC 8 cut(s) 77, 403, 441, 559, 571, 611, 660, 779
Bsp1720I GCTNAGC 1 cut(s) 264
BspACI CCGC 1 cut(s) 667
BspCNI CTCAG 4 cut(s) 36, 172, 256, 726
BspEI TCCGGA 1 cut(s) 250
BspLI GGNNCC 3 cut(s) 108, 573, 613
BspMI ACCTGC 1 cut(s) 19
BspOI GCTAGC 1 cut(s) 800
BspPI GGATC 8 cut(s) 85, 449, 566, 567, 579, 606, 619, 787
BspT107I GGYRCC 1 cut(s) 106
BspTNI GGTCTC 1 cut(s) 237
BsrI ACTGG 3 cut(s) 166, 527, 575
BssMI GATC 8 cut(s) 77, 403, 441, 559, 571, 611, 660, 779
BssSI CACGAG 1 cut(s) 656
Bst2BI CACGAG 1 cut(s) 656
Bst2UI CCWGG 3 cut(s) 432, 609, 675
Bst6I CTCTTC 1 cut(s) 710
BstC8I GCNNGC 3 cut(s) 640, 749, 798
BstDEI CTNAG 4 cut(s) 23, 159, 264, 734
BstF5I GGATG 3 cut(s) 10, 206, 316
BstHHI GCGC 2 cut(s) 449, 468
BstKTI GATC 8 cut(s) 80, 406, 444, 562, 574, 614, 663, 782
BstMAI GTCTC 2 cut(s) 138, 237
BstMBI GATC 8 cut(s) 77, 403, 441, 559, 571, 611, 660, 779
BstMWI GCNNNNNNNGC 1 cut(s) 748
BstNI CCWGG 3 cut(s) 432, 609, 675
BstSCI CCNGG 3 cut(s) 430, 607, 673
BstV1I GCAGC 1 cut(s) 272
BstV2I GAAGAC 1 cut(s) 63
BstX2I RGATCY 3 cut(s) 441, 571, 611
BstYI RGATCY 3 cut(s) 441, 571, 611
BtgZI GCGATG 1 cut(s) 87
BtsCI GGATG 3 cut(s) 10, 206, 316
BtsIMutI CAGTG 2 cut(s) 207, 582
BveI ACCTGC 1 cut(s) 19
Cac8I GCNNGC 3 cut(s) 640, 749, 798
CaiI CAGNNNCTG 1 cut(s) 763
CfoI GCGC 2 cut(s) 449, 468
Cfr13I GGNCC 1 cut(s) 760
CsiI ACCWGGT 1 cut(s) 430
Csp6I GTAC 1 cut(s) 107
CviAII CATG 2 cut(s) 300, 507
CviQI GTAC 1 cut(s) 107
DdeI CTNAG 4 cut(s) 23, 159, 264, 734
DpnI GATC 8 cut(s) 79, 405, 443, 561, 573, 613, 662, 781
DpnII GATC 8 cut(s) 77, 403, 441, 559, 571, 611, 660, 779
Eam1104I CTCTTC 1 cut(s) 710
EarI CTCTTC 1 cut(s) 710
Eco31I GGTCTC 1 cut(s) 237
Eco47I GGWCC 1 cut(s) 760
Eco57I CTGAAG 1 cut(s) 231
EcoO109I RGGNCCY 1 cut(s) 760
EcoRII CCWGG 3 cut(s) 430, 607, 673
EcoT22I ATGCAT 1 cut(s) 519
FaeI CATG 2 cut(s) 303, 510
FaiI YATR 4 cut(s) 233, 301, 508, 792
FatI CATG 2 cut(s) 299, 506
FbaI TGATCA 1 cut(s) 403
Fnu4HI GCNGC 1 cut(s) 261
FokI GGATG 3 cut(s) 17, 213, 303
Fsp4HI GCNGC 1 cut(s) 261
FspBI CTAG 3 cut(s) 497, 554, 797
GlaI GCGC 2 cut(s) 448, 467
GluI GCNGC 1 cut(s) 261
HapII CCGG 1 cut(s) 251
HhaI GCGC 2 cut(s) 449, 468
Hin1II CATG 2 cut(s) 303, 510
Hin6I GCGC 2 cut(s) 447, 466
HinP1I GCGC 2 cut(s) 447, 466
HincII GTYRAC 1 cut(s) 457
HindII GTYRAC 1 cut(s) 457
HindIII AAGCTT 1 cut(s) 740
HinfI GANTC 9 cut(s) 41, 83, 94, 275, 311, 500, 589, 619, 720
HpaII CCGG 1 cut(s) 251
HphI GGTGA 1 cut(s) 446
Hpy166II GTNNAC 2 cut(s) 207, 457
Hpy188III TCNNGA 5 cut(s) 251, 387, 554, 630, 763
Hpy8I GTNNAC 2 cut(s) 207, 457
Hpy99I CGWCG 1 cut(s) 376
HpyAV CCTTC 3 cut(s) 191, 257, 527
HpyCH4IV ACGT 2 cut(s) 453, 804
HpyCH4V TGCA 2 cut(s) 141, 517
HpyF10VI GCNNNNNNNGC 1 cut(s) 748
HpyF3I CTNAG 4 cut(s) 23, 159, 264, 734
HpySE526I ACGT 2 cut(s) 453, 804
Hsp92II CATG 2 cut(s) 303, 510
HspAI GCGC 2 cut(s) 447, 466
Kpn2I TCCGGA 1 cut(s) 250
KpnI GGTACC 1 cut(s) 110
Ksp22I TGATCA 1 cut(s) 403
Kzo9I GATC 8 cut(s) 77, 403, 441, 559, 571, 611, 660, 779
LmnI GCTCC 1 cut(s) 700
Lsp1109I GCAGC 1 cut(s) 272
LweI GCATC 1 cut(s) 504
MabI ACCWGGT 1 cut(s) 430
MaeI CTAG 3 cut(s) 497, 554, 797
MaeII ACGT 2 cut(s) 453, 804
MaeIII GTNAC 1 cut(s) 577
MalI GATC 8 cut(s) 79, 405, 443, 561, 573, 613, 662, 781
MboI GATC 8 cut(s) 77, 403, 441, 559, 571, 611, 660, 779
MboII GAAGA 4 cut(s) 68, 485, 724, 727
MflI RGATCY 3 cut(s) 441, 571, 611
MluCI AATT 3 cut(s) 18, 65, 480
MlyI GAGTC 2 cut(s) 92, 509
MmeI TCCRAC 2 cut(s) 111, 354
MnlI CCTC 9 cut(s) 161, 168, 226, 228, 430, 500, 550, 691, 729
Mph1103I ATGCAT 1 cut(s) 519
MroI TCCGGA 1 cut(s) 250
MseI TTAA 1 cut(s) 811
MspA1I CMGCKG 1 cut(s) 263
MspI CCGG 1 cut(s) 251
MspR9I CCNGG 3 cut(s) 432, 609, 675
MvaI CCWGG 3 cut(s) 432, 609, 675
MwoI GCNNNNNNNGC 1 cut(s) 748
NdeII GATC 8 cut(s) 77, 403, 441, 559, 571, 611, 660, 779
NheI GCTAGC 1 cut(s) 796
NlaIII CATG 2 cut(s) 303, 510
NlaIV GGNNCC 3 cut(s) 108, 573, 613
NmuCI GTSAC 1 cut(s) 577
NsiI ATGCAT 1 cut(s) 519
PaqCI CACCTGC 1 cut(s) 19
PfeI GAWTC 7 cut(s) 41, 94, 275, 311, 589, 619, 720
PkrI GCNGC 1 cut(s) 262
PleI GAGTC 2 cut(s) 91, 508
PpsI GAGTC 2 cut(s) 91, 508
PpuMI RGGWCCY 1 cut(s) 760
Psp5II RGGWCCY 1 cut(s) 760
Psp6I CCWGG 3 cut(s) 430, 607, 673
PspGI CCWGG 3 cut(s) 430, 607, 673
PspN4I GGNNCC 3 cut(s) 108, 573, 613
PspPI GGNCC 1 cut(s) 760
PspPPI RGGWCCY 1 cut(s) 760
PstNI CAGNNNCTG 1 cut(s) 763
PsuI RGATCY 3 cut(s) 441, 571, 611
PvuII CAGCTG 1 cut(s) 263
RsaI GTAC 1 cut(s) 108
RsaNI GTAC 1 cut(s) 107
SaqAI TTAA 1 cut(s) 811
SatI GCNGC 1 cut(s) 261
Sau3AI GATC 8 cut(s) 77, 403, 441, 559, 571, 611, 660, 779
Sau96I GGNCC 1 cut(s) 760
SchI GAGTC 2 cut(s) 92, 509
ScrFI CCNGG 3 cut(s) 432, 609, 675
SexAI ACCWGGT 1 cut(s) 430
SfaNI GCATC 1 cut(s) 504
SinI GGWCC 1 cut(s) 760
Sse9I AATT 3 cut(s) 18, 65, 480
SsiI CCGC 1 cut(s) 667
SspMI CTAG 3 cut(s) 497, 554, 797
StyD4I CCNGG 3 cut(s) 430, 607, 673
TaiI ACGT 2 cut(s) 456, 807
TaqI TCGA 1 cut(s) 324
TasI AATT 3 cut(s) 18, 65, 480
TfiI GAWTC 7 cut(s) 41, 94, 275, 311, 589, 619, 720
Tru1I TTAA 1 cut(s) 811
Tru9I TTAA 1 cut(s) 811
TscAI CASTG 2 cut(s) 214, 582
TseFI GTSAC 1 cut(s) 577
TseI GCWGC 1 cut(s) 260
Tsp45I GTSAC 1 cut(s) 577
TspDTI ATGAA 2 cut(s) 33, 288
TspGWI ACGGA 2 cut(s) 354, 360
TspRI CASTG 2 cut(s) 214, 582
VpaK11BI GGWCC 1 cut(s) 760
XapI RAATTY 1 cut(s) 18
XbaI TCTAGA 1 cut(s) 553
XspI CTAG 3 cut(s) 497, 554, 797
Zsp2I ATGCAT 1 cut(s) 519
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.