FvH4_7g28150

phosphopyruvate hydratase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0002577
Physical Location & Seq
Reverse (-)
20911885 .. 20915823
3939 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g28150.t1

Sequence Viewer

Length: 1341 bp
ATGGCGACGACGATCAAGCTTATCAAGGCCCGTCAGATCTTCGACAGCCGTGGAAATCCCACCGTCGAAGTTGATGTTACTCTCTCCGATGGAACTTTGGCTAGAGCTGCTGTGCCAAGTGGAGCTTCCACAGGTGTGTATGAAGCGCTGGAGTTGAGAGATGGCGGTTCGGATTACCTTGGCAAAGGTGTCCTGAAGGCCGTGGAGAATGTGAATGGCATCATTGGACCTGCTTTGATCGGAAAGGACCCAACGGAACAGACGCAAATTGACAATTACATGGTTCAACAGCTTGATGGAACTACAAATGAATGGGGTTGGTGCAAACAGAAGCTCGGAGCAAATGCTATATTAGCAGTGTCTCTTGCTGTTTGCAAAGCAGGTGCCACTGTGAAAAAGATACCCCTCTACAAGCACATAGCCAATCTTGCTGGAAACAAGACCTTGGTTCTGCCTGTCCCTGCATTCAATGTCATCAATGGAGGTTCTCATGCTGGCAATAAACTAGCTATGCAGGAATTTATGATTCTCCCTGTTGGAGCATCTTCTTTTAAGGAAGCTATGAAGATGGGTGTAGAAGTATATCATCATTTGAAGGCTGTGATTAAGAAGAAGTATGGACAAGATGCCACAAATGTTGGGGATGAAGGTGGCTTTGCACCCAATATTCAGGAAAACAAAGAGGGTCTTGAACTACTGAAGACAGCTATTGCTAAAGCCGGATATACCGGGAAGGTTGTAATTGGGATGGATGTTGCTGCTTCAGAATTCTATGATGATAAGGATAAGACTTATGATTTGAACTTCAAGGAAGAGAAAAATGACGGATCACAAAGAATATCAGGAGACAGTCTGAAAAATGTTTACAAGTCATTTGTTACAGAGTACCCAATTGTGTCAATTGAAGATCCCTTTGATCAGGATGACTGGGAACACTATGCTAAGATGACTGCTGAAATTGGTGAGCAAGTGCAAATTGTTGGTGATGATCTCCTTGTTACAAACCCAAAGCGTGTGGAGAAAGCAATCAAGGAGAAGAGCTGCAATGCCCTTCTTTTGAAGGTGAATCAAATTGGTTCAGTAACCGAAAGTATTGAAGCTGTGAAAATGTCAAAACGTGCTGGATGGGGTGTCATGGCAAGTCACCGAAGTGGTGAAACTGAGGATACTTTCATTGCTGACCTCTCTGTTGGGCTTGCAACGGGCCAGATTAAGACTGGAGCTCCTTGCAGATCAGAGCGCCTTGCTAAATACAACCAGCTTCTTAGAATTGAAGAGGAGCTTGGATCTGCAGCAGTCTATGCTGGATCAAAATTCAGAGTACCAGTGGAGCCGTACTGA

Protein Analysis

447

Amino Acids

48.2

Weight (kDa)

5.85

Isoelectric Point (pI)

36.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Enolase_N PF03952 5 - 140 1.8e-53 Enolase, N-terminal domain
Enolase_C PF00113 149 - 440 3.7e-159 Enolase, C-terminal TIM barrel domain
MR_MLE_C PF13378 275 - 407 2e-08 Enolase C-terminal domain-like
MAAL_C PF07476 300 - 403 8.5e-06 Methylaspartate ammonia-lyase C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 371
Acc36I ACCTGC 2 cut(s) 238, 371
AccB1I GGYRCC 1 cut(s) 383
AciI CCGC 1 cut(s) 165
AclWI GGATC 4 cut(s) 835, 902, 1294, 1315
AcsI RAATTY 3 cut(s) 518, 767, 1313
AcuI CTGAAG 3 cut(s) 215, 719, 747
AfaI GTAC 3 cut(s) 887, 1323, 1337
AfeI AGCGCT 1 cut(s) 147
AjuI GAANNNNNNNTTGG 4 cut(s) 109, 141, 1266, 1298
AleI CACNNNNGTG 2 cut(s) 134, 1149
Alw21I GWGCWC 1 cut(s) 1225
Alw26I GTCTC 2 cut(s) 366, 840
AlwI GGATC 4 cut(s) 835, 902, 1294, 1315
Aor51HI AGCGCT 1 cut(s) 147
AoxI GGCC 3 cut(s) 27, 198, 1204
ApeKI GCWGC 4 cut(s) 107, 758, 1041, 1292
ApoI RAATTY 3 cut(s) 518, 767, 1313
ArsI GACNNNNNNTTYG 2 cut(s) 781, 813
AspLEI GCGC 2 cut(s) 148, 1242
AspS9I GGNCC 4 cut(s) 28, 227, 247, 1204
AsuC2I CCSGG 1 cut(s) 730
AsuHPI GGTGA 5 cut(s) 974, 995, 1075, 1136, 1166
AvaII GGWCC 2 cut(s) 227, 247
BanI GGYRCC 1 cut(s) 383
BanII GRGCYC 1 cut(s) 1225
BbsI GAAGAC 1 cut(s) 707
Bbv12I GWGCWC 1 cut(s) 1225
BbvI GCAGC 4 cut(s) 94, 745, 1028, 1304
BccI CCATC 6 cut(s) 83, 155, 290, 562, 742, 1119
BceAI ACGGC 3 cut(s) 33, 185, 1318
BciVI GTATCC 1 cut(s) 1159
BclI TGATCA 1 cut(s) 916
BcnI CCSGG 1 cut(s) 730
BcoDI GTCTC 2 cut(s) 366, 840
BfaI CTAG 2 cut(s) 102, 506
BfmI CTRYAG 1 cut(s) 1290
BfoI RGCGCY 2 cut(s) 149, 1243
BfuAI ACCTGC 2 cut(s) 238, 371
BfuI GTATCC 1 cut(s) 1159
BglII AGATCT 1 cut(s) 36
BisI GCNGC 4 cut(s) 108, 759, 1042, 1293
BlsI GCNGC 4 cut(s) 109, 760, 1043, 1294
Bme1390I CCNGG 1 cut(s) 730
Bme18I GGWCC 2 cut(s) 227, 247
BmgT120I GGNCC 4 cut(s) 28, 227, 247, 1204
BmiI GGNNCC 3 cut(s) 249, 385, 1332
BmrFI CCNGG 1 cut(s) 730
BmrI ACTGGG 1 cut(s) 937
BmsI GCATC 3 cut(s) 228, 551, 616
BmuI ACTGGG 1 cut(s) 937
BpiI GAAGAC 1 cut(s) 707
BpmI CTGGAG 2 cut(s) 170, 1239
BpuMI CCSGG 1 cut(s) 730
BsaJI CCNNGG 4 cut(s) 49, 178, 201, 444
BsaXI ACNNNNNCTCC 2 cut(s) 1207, 1237
Bse1I ACTGG 3 cut(s) 932, 1222, 1325
Bse3DI GCAATG 2 cut(s) 1051, 1173
BseDI CCNNGG 4 cut(s) 49, 178, 201, 444
BseGI GGATG 5 cut(s) 649, 753, 757, 928, 1130
BseMI GCAATG 2 cut(s) 1051, 1173
BseMII CTCAG 1 cut(s) 1152
BseNI ACTGG 3 cut(s) 932, 1222, 1325
BseRI GAGGAG 1 cut(s) 1292
BseXI GCAGC 4 cut(s) 94, 745, 1028, 1304
BshFI GGCC 3 cut(s) 29, 200, 1206
BshNI GGYRCC 1 cut(s) 383
BsiHKAI GWGCWC 1 cut(s) 1225
BsiSI CCGG 2 cut(s) 720, 729
BslFI GGGAC 1 cut(s) 443
BsmAI GTCTC 2 cut(s) 366, 840
BsmFI GGGAC 1 cut(s) 443
BsmI GAATGC 1 cut(s) 464
BsnI GGCC 3 cut(s) 29, 200, 1206
Bsp1286I GDGCHC 1 cut(s) 1225
BspACI CCGC 1 cut(s) 165
BspANI GGCC 3 cut(s) 29, 200, 1206
BspCNI CTCAG 1 cut(s) 1153
BspLI GGNNCC 3 cut(s) 249, 385, 1332
BspMAI CTGCAG 1 cut(s) 1294
BspMI ACCTGC 2 cut(s) 238, 371
BspPI GGATC 4 cut(s) 835, 902, 1294, 1315
BspQI GCTCTTC 1 cut(s) 1031
BspT107I GGYRCC 1 cut(s) 383
BsrDI GCAATG 2 cut(s) 1051, 1173
BsrI ACTGG 3 cut(s) 932, 1222, 1325
BssECI CCNNGG 4 cut(s) 49, 178, 201, 444
BssT1I CCWWGG 2 cut(s) 178, 444
Bst4CI ACNGT 3 cut(s) 64, 391, 851
Bst6I CTCTTC 3 cut(s) 807, 1031, 1269
BstAPI GCANNNNNTGC 1 cut(s) 1301
BstC8I GCNNGC 2 cut(s) 496, 1197
BstDEI CTNAG 3 cut(s) 942, 1161, 1265
BstDSI CCRYGG 2 cut(s) 49, 201
BstF5I GGATG 5 cut(s) 649, 753, 757, 928, 1130
BstH2I RGCGCY 2 cut(s) 149, 1243
BstHHI GCGC 2 cut(s) 148, 1242
BstMAI GTCTC 2 cut(s) 366, 840
BstMWI GCNNNNNNNGC 4 cut(s) 107, 353, 428, 1301
BstSCI CCNGG 1 cut(s) 728
BstSFI CTRYAG 1 cut(s) 1290
BstV1I GCAGC 4 cut(s) 94, 745, 1028, 1304
BstV2I GAAGAC 1 cut(s) 707
BstX2I RGATCY 3 cut(s) 36, 907, 1286
BstYI RGATCY 3 cut(s) 36, 907, 1286
BsuI GTATCC 1 cut(s) 1159
BsuRI GGCC 3 cut(s) 29, 200, 1206
BtgI CCRYGG 2 cut(s) 49, 201
BtsCI GGATG 5 cut(s) 649, 753, 757, 928, 1130
BtsI GCAGTG 1 cut(s) 363
BtsIMutI CAGTG 3 cut(s) 363, 387, 1332
BveI ACCTGC 2 cut(s) 238, 371
Cac8I GCNNGC 2 cut(s) 496, 1197
CfoI GCGC 2 cut(s) 148, 1242
Cfr13I GGNCC 4 cut(s) 28, 227, 247, 1204
CseI GACGC 1 cut(s) 271
Csp6I GTAC 3 cut(s) 886, 1322, 1336
CspCI CAANNNNNGTGG 4 cut(s) 619, 654, 996, 1031
CviAII CATG 3 cut(s) 280, 491, 1135
CviQI GTAC 3 cut(s) 886, 1322, 1336
DdeI CTNAG 3 cut(s) 942, 1161, 1265
Eam1104I CTCTTC 3 cut(s) 807, 1031, 1269
EarI CTCTTC 3 cut(s) 807, 1031, 1269
Ecl136II GAGCTC 1 cut(s) 1223
Eco130I CCWWGG 2 cut(s) 178, 444
Eco24I GRGCYC 1 cut(s) 1225
Eco47I GGWCC 2 cut(s) 227, 247
Eco47III AGCGCT 1 cut(s) 147
Eco53kI GAGCTC 1 cut(s) 1223
Eco57I CTGAAG 3 cut(s) 215, 719, 747
EcoICRI GAGCTC 1 cut(s) 1223
EcoO109I RGGNCCY 1 cut(s) 247
EcoRI GAATTC 1 cut(s) 767
EcoT14I CCWWGG 2 cut(s) 178, 444
EcoT38I GRGCYC 1 cut(s) 1225
ErhI CCWWGG 2 cut(s) 178, 444
FaeI CATG 3 cut(s) 283, 494, 1138
FalI AAGNNNNNCTT 6 cut(s) 109, 141, 672, 704, 1266, 1298
FaqI GGGAC 1 cut(s) 443
FatI CATG 3 cut(s) 279, 490, 1134
FbaI TGATCA 1 cut(s) 916
Fnu4HI GCNGC 4 cut(s) 108, 759, 1042, 1293
FokI GGATG 5 cut(s) 656, 760, 764, 935, 1137
FriOI GRGCYC 1 cut(s) 1225
Fsp4HI GCNGC 4 cut(s) 108, 759, 1042, 1293
FspBI CTAG 2 cut(s) 102, 506
GlaI GCGC 2 cut(s) 147, 1241
GluI GCNGC 4 cut(s) 108, 759, 1042, 1293
GsuI CTGGAG 2 cut(s) 170, 1239
HaeII RGCGCY 2 cut(s) 149, 1243
HaeIII GGCC 3 cut(s) 29, 200, 1206
HapII CCGG 2 cut(s) 720, 729
HgaI GACGC 1 cut(s) 271
HhaI GCGC 2 cut(s) 148, 1242
Hin1II CATG 3 cut(s) 283, 494, 1138
Hin6I GCGC 2 cut(s) 146, 1240
HinP1I GCGC 2 cut(s) 146, 1240
HindIII AAGCTT 1 cut(s) 17
HinfI GANTC 2 cut(s) 526, 1066
HpaII CCGG 2 cut(s) 720, 729
HphI GGTGA 5 cut(s) 974, 995, 1075, 1136, 1166
Hpy166II GTNNAC 1 cut(s) 865
Hpy188I TCNGA 9 cut(s) 36, 88, 172, 242, 338, 766, 855, 1237, 1319
Hpy188III TCNNGA 5 cut(s) 193, 671, 689, 843, 920
Hpy8I GTNNAC 1 cut(s) 865
Hpy99I CGWCG 3 cut(s) 10, 13, 68
HpyAV CCTTC 6 cut(s) 190, 589, 641, 727, 1054, 1061
HpyCH4III ACNGT 3 cut(s) 64, 391, 851
HpyCH4IV ACGT 1 cut(s) 1117
HpyF10VI GCNNNNNNNGC 4 cut(s) 107, 353, 428, 1301
HpyF3I CTNAG 3 cut(s) 942, 1161, 1265
HpySE526I ACGT 1 cut(s) 1117
Hsp92II CATG 3 cut(s) 283, 494, 1138
HspAI GCGC 2 cut(s) 146, 1240
Ksp22I TGATCA 1 cut(s) 916
LguI GCTCTTC 1 cut(s) 1031
LmnI GCTCC 7 cut(s) 122, 338, 539, 1220, 1228, 1279, 1330
Lsp1109I GCAGC 4 cut(s) 94, 745, 1028, 1304
LweI GCATC 3 cut(s) 228, 551, 616
MaeI CTAG 2 cut(s) 102, 506
MaeII ACGT 1 cut(s) 1117
MaeIII GTNAC 5 cut(s) 76, 877, 997, 1081, 1142
MboII GAAGA 9 cut(s) 31, 537, 577, 622, 712, 824, 917, 1048, 1286
MfeI CAATTG 2 cut(s) 891, 900
MflI RGATCY 3 cut(s) 36, 907, 1286
MhlI GDGCHC 1 cut(s) 1225
MmeI TCCRAC 1 cut(s) 517
MnlI CCTC 6 cut(s) 416, 476, 676, 1156, 1193, 1270
MseI TTAA 3 cut(s) 552, 606, 1212
MslI CAYNNNNRTG 2 cut(s) 134, 1149
MspI CCGG 2 cut(s) 720, 729
MspR9I CCNGG 1 cut(s) 730
MunI CAATTG 2 cut(s) 891, 900
Mva1269I GAATGC 1 cut(s) 464
MwoI GCNNNNNNNGC 4 cut(s) 107, 353, 428, 1301
NciI CCSGG 1 cut(s) 730
NlaIII CATG 3 cut(s) 283, 494, 1138
NlaIV GGNNCC 3 cut(s) 249, 385, 1332
NmuCI GTSAC 1 cut(s) 1142
OliI CACNNNNGTG 2 cut(s) 134, 1149
PaqCI CACCTGC 1 cut(s) 371
PciSI GCTCTTC 1 cut(s) 1031
PctI GAATGC 1 cut(s) 464
PfeI GAWTC 2 cut(s) 526, 1066
PkrI GCNGC 4 cut(s) 109, 760, 1043, 1294
PpuMI RGGWCCY 1 cut(s) 247
Psp124BI GAGCTC 1 cut(s) 1225
Psp5II RGGWCCY 1 cut(s) 247
PspN4I GGNNCC 3 cut(s) 249, 385, 1332
PspPI GGNCC 4 cut(s) 28, 227, 247, 1204
PspPPI RGGWCCY 1 cut(s) 247
PstI CTGCAG 1 cut(s) 1294
PsuI RGATCY 3 cut(s) 36, 907, 1286
RsaI GTAC 3 cut(s) 887, 1323, 1337
RsaNI GTAC 3 cut(s) 886, 1322, 1336
RseI CAYNNNNRTG 2 cut(s) 134, 1149
SacI GAGCTC 1 cut(s) 1225
SapI GCTCTTC 1 cut(s) 1031
SaqAI TTAA 3 cut(s) 552, 606, 1212
SatI GCNGC 4 cut(s) 108, 759, 1042, 1293
Sau96I GGNCC 4 cut(s) 28, 227, 247, 1204
ScrFI CCNGG 1 cut(s) 730
SduI GDGCHC 1 cut(s) 1225
SfaNI GCATC 3 cut(s) 228, 551, 616
SfcI CTRYAG 1 cut(s) 1290
SinI GGWCC 2 cut(s) 227, 247
SmiMI CAYNNNNRTG 2 cut(s) 134, 1149
SsiI CCGC 1 cut(s) 165
SspI AATATT 1 cut(s) 667
SspMI CTAG 2 cut(s) 102, 506
SstI GAGCTC 1 cut(s) 1225
StyD4I CCNGG 1 cut(s) 728
StyI CCWWGG 2 cut(s) 178, 444
TaaI ACNGT 3 cut(s) 64, 391, 851
TaiI ACGT 1 cut(s) 1120
TaqI TCGA 2 cut(s) 42, 66
TfiI GAWTC 2 cut(s) 526, 1066
Tru1I TTAA 3 cut(s) 552, 606, 1212
Tru9I TTAA 3 cut(s) 552, 606, 1212
TscAI CASTG 3 cut(s) 363, 394, 1332
TseFI GTSAC 1 cut(s) 1142
TseI GCWGC 4 cut(s) 107, 758, 1041, 1292
Tsp45I GTSAC 1 cut(s) 1142
TspDTI ATGAA 5 cut(s) 156, 324, 578, 660, 1162
TspGWI ACGGA 2 cut(s) 269, 840
TspRI CASTG 3 cut(s) 363, 394, 1332
VpaK11BI GGWCC 2 cut(s) 227, 247
XapI RAATTY 3 cut(s) 518, 767, 1313
XcmI CCANNNNNNNNNTGG 1 cut(s) 1214
XspI CTAG 2 cut(s) 102, 506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.