Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0002577

Physical Location & Seq

Genomic Coordinates Forward (+)

1648150 .. 1651933

Gene Structure 3784 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1016600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1335 bp

ATGGCGACGATCCAGAGCGTGAAGGCTAGGCAGATCTTCGACAGCCGTGGCAATCCCACCGTCGAGGTCGATATCATACTGTCCGATGGCACTTTGGCCAGAGCCGCCGTTCCAAGTGGTGCATCCACCGGAGTTTACGAGGCCCTTGAGTTGCGCGATGGAGGTAAAGACTACCTCGGAAAGGGTGTATCGAAGGCTGTGAACAATGTCAACTCAATCATCGGCCCTGCTTTGATCGGCAAGGACCCAAGTGAACAGACTGCTATTGACAACTTCATGGTTCAACAGCTCGATGGAACTGTTAACGAGTGGGGTTGGTGCAAACAAAAGCTTGGTGCAAATGCCATCTTGGCCGTGTCTCTTGCAGTCGCCAAGGCTGGCGCTAGCGTCAAGAAAATTCCTCTGTACAAGCATATTGCCAATCTTGCTGGCAACAATAACTTAGTTTTGCCCGTTCCTGCTTTCAATGTCATCAACGGTGGATCACATGCAGGAAACAAGCTTGCAATGCAGGAGTTCATGATTCTTCCTGTTGGAGCTTCCTCATTCAAGGAAGCCATGAAGATGGGCGTGGAAGTATATCACAACCTTAAGTCTGTGATTAAAAAGAAGTACGGTCAGGATGCAACCAATGTTGGTGACGAAGGTGGATTTGCTCCTAACATCCAGGAGAACAAGGAAGGTCTTGAATTGCTCAAGACAGCCATTGAGAAGGCTGGATACACCGGCAAAGTTGTCATTGGAATGGACGTTGCTGCATCTGAGTTTTATGGATCAGATAAAACCTATGATCTGAACTTCAAGGAAGAGAAAAATGACGGTTCCCAAAAGATCTTAGGAAATGCTCTCAAGGACCTCTACAAGTCATTTGCAAGTGAGTACCCTATTGTTTCAATTGAAGATCCATTTGACCAAGATGACTGGGAACACTATGCCAAGATGACAGCTGAAGTTGGAGAAAAAGTACAAATTGTGGGAGATGATCTGTTGGTCACCAACCCCAAGAGGGTTGAGAAGGCAATCAAGGAGAAATCTTGCAATGCTCTTCTTCTCAAGGTGAATCAAATTGGATCCGTAACTGAGAGCATTGAGGCTGTAAGAATGTCCAAGAAAGCTGGATGGGGTGTGATGGCCAGCCACCGCAGTGGAGAAACCGAGGATACCTTCATTGCTGATCTCTCCGTGGGTTTGGCCACGGGACAAATCAAGACAGGAGCTCCCTGTAGGTCAGAGCGTCTTGCCAAGTACAACCAGCTCTTGCGAATTGAGGAGGAGCTCGGTTCAGAGGCAGTGTATGCTGGAGCCAAGTTCCGTGTGCCCGTGGAGCCCTATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004634 GO:0005488 GO:0005507 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005740 GO:0005829 GO:0005886 GO:0005911 GO:0006950 GO:0006970 GO:0008150 GO:0009266 GO:0009314 GO:0009409 GO:0009416 GO:0009506 GO:0009507 GO:0009536 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0010033 GO:0010035 GO:0010038 GO:0016020 GO:0016829 GO:0016835 GO:0016836 GO:0030054 GO:0031967 GO:0031975 GO:0033993 GO:0042221 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0046686 GO:0046872 GO:0046914 GO:0048046 GO:0050896 GO:0055044 GO:0071944 GO:0097159 GO:0097305 GO:1901363 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00010 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko03018 ko04066 map00010 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map03018 map04066

Pfam Domains

Protein Families

Enolase_C Enolase_N

Protein Analysis

445

Amino Acids

47.8

Weight (kDa)

5.77

Isoelectric Point (pI)

36.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Enolase_N	PF03952	4 - 139	2.6e-53	Enolase, N-terminal domain
Enolase_C	PF00113	148 - 438	7.6e-159	Enolase, C-terminal TIM barrel domain
MAAL_C	PF07476	291 - 401	2.7e-06	Methylaspartate ammonia-lyase C-terminus
MR_MLE_C	PF13378	292 - 406	1.6e-08	Enolase C-terminal domain-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002577)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G36530 AT2G36530
fragaria_vesca	FvH4_6g17770 FvH4_6g17770 FvH4_7g28150
malus_domestica	MD01G1185500.v1.1 MD12G1016600.v1.1 MD14G1014600.v1.1
prunus_persica	Prupe.2G281900_v2.0.a1 Prupe.2G281900_v2.0.a1 Prupe.7G118700_v2.0.a1
pyrus_communis	pycom01g19710 pycom12g01360 pycom14g01220
rosa_chinensis	RchiOBHm_Chr1g0375091 RchiOBHm_Chr3g0472001
rosa_laevigata	RLG00000000873 RLG00000024113
rosa_multiflora	Rmu_sc0000090.1_g000028 Rmu_sc0000554.1_g000017 Rmu_sc0002532.1_g000001 Rmu_sc0034562.1_g000001 Rmu_sc0039689.1_g000001
rosa_roxburghii	Rroxscaffold_4G00283020 Rroxscaffold_6G00409370
rosa_rugosa	Rorug01G0388300 Rorug01G0388400 Rorug03G0121500 Rorug03G0121600
rosa_samantha	Rh1AG398800 Rh1BG365700 Rh1CG378600 Rh1DG396400 Rh3AG173200 Rh3BG198600 Rh3CG189800 Rh3CG197100 Rh3DG175800
rosa_wichuraiana	Rw1G035410 Rw3G016750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	1 cut(s)	156
AciI	CCGC	2 cut(s)	105, 1141
AclWI	GGATC	6 cut(s)	4, 490, 781, 896, 1065, 1078
AcoI	YGGCCR	4 cut(s)	96, 351, 1131, 1191
AcsI	RAATTY	1 cut(s)	396
AcuI	CTGAAG	1 cut(s)	969
AfaI	GTAC	5 cut(s)	407, 614, 881, 966, 1247
AfiI	CCNNNNNNNGG	2 cut(s)	181, 1006
AflII	CTTAAG	1 cut(s)	590
AgsI	TTSAA	7 cut(s)	284, 466, 550, 689, 802, 894, 899
AjnI	CCWGG	1 cut(s)	666
AleI	CACNNNNGTG	1 cut(s)	1143
AluBI	AGCT	9 cut(s)	289, 331, 502, 539, 947, 1115, 1217, 1255, 1276
AluI	AGCT	9 cut(s)	289, 331, 502, 539, 947, 1115, 1217, 1255, 1276
Alw21I	GWGCWC	2 cut(s)	1219, 1278
Alw26I	GTCTC	1 cut(s)	363
AlwI	GGATC	6 cut(s)	4, 490, 781, 896, 1065, 1078
AoxI	GGCC	6 cut(s)	96, 141, 223, 351, 1131, 1191
ApeKI	GCWGC	1 cut(s)	755
ApoI	RAATTY	1 cut(s)	396
AspLEI	GCGC	2 cut(s)	156, 383
AspS9I	GGNCC	4 cut(s)	142, 224, 244, 853
AsuHPI	GGTGA	3 cut(s)	650, 985, 1069
AsuNHI	GCTAGC	1 cut(s)	383
AvaII	GGWCC	2 cut(s)	244, 853
BaeGI	GKGCMC	1 cut(s)	1320
BalI	TGGCCA	3 cut(s)	98, 1133, 1193
BamHI	GGATCC	1 cut(s)	1070
BanII	GRGCYC	3 cut(s)	1219, 1278, 1329
BarI	GAAGNNNNNNTAC	2 cut(s)	704, 736
Bbv12I	GWGCWC	2 cut(s)	1219, 1278
BbvI	GCAGC	1 cut(s)	742
BccI	CCATC	7 cut(s)	80, 152, 287, 353, 559, 1113, 1123
BceAI	ACGGC	3 cut(s)	30, 92, 338
BciT130I	CCWGG	1 cut(s)	668
BciVI	GTATCC	2 cut(s)	713, 1153
BcoDI	GTCTC	1 cut(s)	363
BfaI	CTAG	2 cut(s)	27, 384
BfmI	CTRYAG	1 cut(s)	1222
BfoI	RGCGCY	1 cut(s)	384
BfrI	CTTAAG	1 cut(s)	590
BfuI	GTATCC	2 cut(s)	713, 1153
BglI	GCCNNNNNGGC	1 cut(s)	350
BglII	AGATCT	2 cut(s)	33, 831
BisI	GCNGC	2 cut(s)	105, 756
BlsI	GCNGC	2 cut(s)	106, 757
Bme1390I	CCNGG	1 cut(s)	668
Bme18I	GGWCC	2 cut(s)	244, 853
BmgT120I	GGNCC	4 cut(s)	142, 224, 244, 853
BmiI	GGNNCC	5 cut(s)	246, 823, 1072, 1303, 1326
BmrFI	CCNGG	1 cut(s)	668
BmrI	ACTGGG	1 cut(s)	931
BmsI	GCATC	3 cut(s)	131, 613, 767
BmtI	GCTAGC	1 cut(s)	387
BmuI	ACTGGG	1 cut(s)	931
BpmI	CTGGAG	1 cut(s)	1320
BpuEI	CTTGAG	4 cut(s)	167, 680, 833, 1037
BsaJI	CCNNGG	7 cut(s)	46, 175, 372, 1155, 1182, 1194, 1320
BsaWI	WCCGGW	1 cut(s)	128
BsaXI	ACNNNNNCTCC	4 cut(s)	948, 978, 1201, 1231
Bsc4I	CCNNNNNNNGG	2 cut(s)	181, 1006
Bse118I	RCCGGY	1 cut(s)	725
Bse1I	ACTGG	1 cut(s)	926
Bse3DI	GCAATG	3 cut(s)	513, 1045, 1167
BseBI	CCWGG	1 cut(s)	668
BseDI	CCNNGG	7 cut(s)	46, 175, 372, 1155, 1182, 1194, 1320
BseGI	GGATG	4 cut(s)	122, 628, 663, 1124
BseLI	CCNNNNNNNGG	2 cut(s)	181, 1006
BseMI	GCAATG	3 cut(s)	513, 1045, 1167
BseMII	CTCAG	2 cut(s)	753, 1071
BseNI	ACTGG	1 cut(s)	926
BseRI	GAGGAG	2 cut(s)	1283, 1286
BseSI	GKGCMC	1 cut(s)	1320
BseXI	GCAGC	1 cut(s)	742
Bsh1236I	CGCG	1 cut(s)	156
BshFI	GGCC	6 cut(s)	98, 143, 225, 353, 1133, 1193
BsiHKAI	GWGCWC	2 cut(s)	1219, 1278
BsiSI	CCGG	2 cut(s)	129, 726
BslFI	GGGAC	1 cut(s)	1212
BslI	CCNNNNNNNGG	2 cut(s)	181, 1006
BsmAI	GTCTC	1 cut(s)	363
BsmFI	GGGAC	1 cut(s)	1212
BsnI	GGCC	6 cut(s)	98, 143, 225, 353, 1133, 1193
Bsp1286I	GDGCHC	4 cut(s)	1219, 1278, 1320, 1329
Bsp1407I	TGTACA	1 cut(s)	405
BspACI	CCGC	2 cut(s)	105, 1141
BspANI	GGCC	6 cut(s)	98, 143, 225, 353, 1133, 1193
BspCNI	CTCAG	2 cut(s)	754, 1072
BspFNI	CGCG	1 cut(s)	156
BspHI	TCATGA	1 cut(s)	519
BspLI	GGNNCC	5 cut(s)	246, 823, 1072, 1303, 1326
BspOI	GCTAGC	1 cut(s)	387
BspPI	GGATC	6 cut(s)	4, 490, 781, 896, 1065, 1078
BspQI	GCTCTTC	1 cut(s)	1050
BspTI	CTTAAG	1 cut(s)	590
BsrDI	GCAATG	3 cut(s)	513, 1045, 1167
BsrFI	RCCGGY	1 cut(s)	725
BsrGI	TGTACA	1 cut(s)	405
BsrI	ACTGG	1 cut(s)	926
BssAI	RCCGGY	1 cut(s)	725
BssECI	CCNNGG	7 cut(s)	46, 175, 372, 1155, 1182, 1194, 1320
BssT1I	CCWWGG	1 cut(s)	372
Bst2UI	CCWGG	1 cut(s)	668
Bst4CI	ACNGT	6 cut(s)	61, 81, 301, 479, 617, 821
Bst6I	CTCTTC	2 cut(s)	801, 1050
BstAFI	CTTAAG	1 cut(s)	590
BstAPI	GCANNNNNTGC	1 cut(s)	1295
BstAUI	TGTACA	1 cut(s)	405
BstC8I	GCNNGC	5 cut(s)	379, 385, 430, 504, 1135
BstDEI	CTNAG	4 cut(s)	442, 762, 835, 1080
BstDSI	CCRYGG	4 cut(s)	46, 1182, 1194, 1320
BstEII	GGTNACC	1 cut(s)	991
BstENI	CCTNNNNNAGG	1 cut(s)	179
BstF5I	GGATG	4 cut(s)	122, 628, 663, 1124
BstFNI	CGCG	1 cut(s)	156
BstH2I	RGCGCY	1 cut(s)	384
BstHHI	GCGC	2 cut(s)	156, 383
BstMAI	GTCTC	1 cut(s)	363
BstMWI	GCNNNNNNNGC	6 cut(s)	104, 350, 425, 508, 1295, 1324
BstNI	CCWGG	1 cut(s)	668
BstNSI	RCATGY	1 cut(s)	491
BstPI	GGTNACC	1 cut(s)	991
BstSCI	CCNGG	1 cut(s)	666
BstSFI	CTRYAG	1 cut(s)	1222
BstSLI	GKGCMC	1 cut(s)	1320
BstUI	CGCG	1 cut(s)	156
BstV1I	GCAGC	1 cut(s)	742
BstX2I	RGATCY	4 cut(s)	33, 831, 901, 1070
BstXI	CCANNNNNNTGG	2 cut(s)	565, 1145
BstYI	RGATCY	4 cut(s)	33, 831, 901, 1070
BsuI	GTATCC	2 cut(s)	713, 1153
BsuRI	GGCC	6 cut(s)	98, 143, 225, 353, 1133, 1193
BtgI	CCRYGG	4 cut(s)	46, 1182, 1194, 1320
BtgZI	GCGATG	1 cut(s)	171
BtsCI	GGATG	4 cut(s)	122, 628, 663, 1124
BtsI	GCAGTG	2 cut(s)	1150, 1296
BtsIMutI	CAGTG	2 cut(s)	1150, 1296
Cac8I	GCNNGC	5 cut(s)	379, 385, 430, 504, 1135
CciI	TCATGA	1 cut(s)	519
CfoI	GCGC	2 cut(s)	156, 383
Cfr10I	RCCGGY	1 cut(s)	725
Cfr13I	GGNCC	4 cut(s)	142, 224, 244, 853
CseI	GACGC	2 cut(s)	376, 1223
Csp6I	GTAC	5 cut(s)	406, 613, 880, 965, 1246
CviAII	CATG	4 cut(s)	277, 488, 520, 559
CviQI	GTAC	5 cut(s)	406, 613, 880, 965, 1246
DdeI	CTNAG	4 cut(s)	442, 762, 835, 1080
EaeI	YGGCCR	4 cut(s)	96, 351, 1131, 1191
Eam1104I	CTCTTC	2 cut(s)	801, 1050
EarI	CTCTTC	2 cut(s)	801, 1050
Ecl136II	GAGCTC	2 cut(s)	1217, 1276
Eco130I	CCWWGG	1 cut(s)	372
Eco24I	GRGCYC	3 cut(s)	1219, 1278, 1329
Eco32I	GATATC	1 cut(s)	73
Eco47I	GGWCC	2 cut(s)	244, 853
Eco53kI	GAGCTC	2 cut(s)	1217, 1276
Eco57I	CTGAAG	1 cut(s)	969
Eco91I	GGTNACC	1 cut(s)	991
EcoICRI	GAGCTC	2 cut(s)	1217, 1276
EcoNI	CCTNNNNNAGG	1 cut(s)	179
EcoO109I	RGGNCCY	3 cut(s)	142, 244, 853
EcoO65I	GGTNACC	1 cut(s)	991
EcoRII	CCWGG	1 cut(s)	666
EcoRV	GATATC	1 cut(s)	73
EcoT14I	CCWWGG	1 cut(s)	372
EcoT38I	GRGCYC	3 cut(s)	1219, 1278, 1329
ErhI	CCWWGG	1 cut(s)	372
FaeI	CATG	4 cut(s)	280, 491, 523, 562
FaqI	GGGAC	1 cut(s)	1212
FatI	CATG	4 cut(s)	276, 487, 519, 558
Fnu4HI	GCNGC	2 cut(s)	105, 756
FokI	GGATG	4 cut(s)	109, 635, 650, 1131
FriOI	GRGCYC	3 cut(s)	1219, 1278, 1329
Fsp4HI	GCNGC	2 cut(s)	105, 756
FspBI	CTAG	2 cut(s)	27, 384
GlaI	GCGC	2 cut(s)	155, 382
GluI	GCNGC	2 cut(s)	105, 756
GsuI	CTGGAG	1 cut(s)	1320
HaeII	RGCGCY	1 cut(s)	384
HaeIII	GGCC	6 cut(s)	98, 143, 225, 353, 1133, 1193
HapII	CCGG	2 cut(s)	129, 726
HgaI	GACGC	2 cut(s)	376, 1223
HhaI	GCGC	2 cut(s)	156, 383
Hin1II	CATG	4 cut(s)	280, 491, 523, 562
Hin6I	GCGC	2 cut(s)	154, 381
HinP1I	GCGC	2 cut(s)	154, 381
HincII	GTYRAC	2 cut(s)	211, 304
HindII	GTYRAC	2 cut(s)	211, 304
HindIII	AAGCTT	2 cut(s)	329, 500
HinfI	GANTC	2 cut(s)	523, 1060
HpaI	GTTAAC	1 cut(s)	304
HpaII	CCGG	2 cut(s)	129, 726
HphI	GGTGA	3 cut(s)	650, 985, 1069
Hpy166II	GTNNAC	5 cut(s)	136, 202, 211, 254, 304
Hpy188I	TCNGA	7 cut(s)	85, 179, 763, 778, 795, 1231, 1285
Hpy188III	TCNNGA	7 cut(s)	13, 391, 520, 620, 686, 697, 1207
Hpy8I	GTNNAC	5 cut(s)	136, 202, 211, 254, 304
Hpy99I	CGWCG	2 cut(s)	10, 65
HpyAV	CCTTC	7 cut(s)	16, 187, 638, 674, 706, 1009, 1174
HpyCH4III	ACNGT	6 cut(s)	61, 81, 301, 479, 617, 821
HpyCH4IV	ACGT	1 cut(s)	750
HpyF10VI	GCNNNNNNNGC	6 cut(s)	104, 350, 425, 508, 1295, 1324
HpyF3I	CTNAG	4 cut(s)	442, 762, 835, 1080
HpySE526I	ACGT	1 cut(s)	750
Hsp92II	CATG	4 cut(s)	280, 491, 523, 562
HspAI	GCGC	2 cut(s)	154, 381
KspAI	GTTAAC	1 cut(s)	304
LguI	GCTCTTC	1 cut(s)	1050
LmnI	GCTCC	7 cut(s)	536, 661, 1214, 1222, 1273, 1301, 1324
Lsp1109I	GCAGC	1 cut(s)	742
LweI	GCATC	3 cut(s)	131, 613, 767
MaeI	CTAG	2 cut(s)	27, 384
MaeII	ACGT	1 cut(s)	750
MaeIII	GTNAC	3 cut(s)	638, 991, 1075
MboII	GAAGA	7 cut(s)	28, 518, 574, 818, 911, 1037, 1040
MfeI	CAATTG	1 cut(s)	894
MflI	RGATCY	4 cut(s)	33, 831, 901, 1070
MhlI	GDGCHC	4 cut(s)	1219, 1278, 1320, 1329
MlsI	TGGCCA	3 cut(s)	98, 1133, 1193
MluCI	AATT	6 cut(s)	396, 689, 894, 969, 1065, 1263
MluNI	TGGCCA	3 cut(s)	98, 1133, 1193
MmeI	TCCRAC	2 cut(s)	514, 934
Mox20I	TGGCCA	3 cut(s)	98, 1133, 1193
MscI	TGGCCA	3 cut(s)	98, 1133, 1193
MseI	TTAA	3 cut(s)	303, 591, 603
MslI	CAYNNNNRTG	3 cut(s)	563, 743, 1143
Msp20I	TGGCCA	3 cut(s)	98, 1133, 1193
MspA1I	CMGCKG	1 cut(s)	947
MspCI	CTTAAG	1 cut(s)	590
MspI	CCGG	2 cut(s)	129, 726
MspR9I	CCNGG	1 cut(s)	668
MunI	CAATTG	1 cut(s)	894
MvaI	CCWGG	1 cut(s)	668
MvnI	CGCG	1 cut(s)	156
MwoI	GCNNNNNNNGC	6 cut(s)	104, 350, 425, 508, 1295, 1324
NheI	GCTAGC	1 cut(s)	383
NlaIII	CATG	4 cut(s)	280, 491, 523, 562
NlaIV	GGNNCC	5 cut(s)	246, 823, 1072, 1303, 1326
NmuCI	GTSAC	2 cut(s)	638, 991
NspI	RCATGY	1 cut(s)	491
OliI	CACNNNNGTG	1 cut(s)	1143
PagI	TCATGA	1 cut(s)	519
PciSI	GCTCTTC	1 cut(s)	1050
PfeI	GAWTC	2 cut(s)	523, 1060
PfoI	TCCNGGA	1 cut(s)	666
PkrI	GCNGC	2 cut(s)	106, 757
PpuMI	RGGWCCY	2 cut(s)	244, 853
Psp124BI	GAGCTC	2 cut(s)	1219, 1278
Psp5II	RGGWCCY	2 cut(s)	244, 853
Psp6I	CCWGG	1 cut(s)	666
PspEI	GGTNACC	1 cut(s)	991
PspGI	CCWGG	1 cut(s)	666
PspN4I	GGNNCC	5 cut(s)	246, 823, 1072, 1303, 1326
PspPI	GGNCC	4 cut(s)	142, 224, 244, 853
PspPPI	RGGWCCY	2 cut(s)	244, 853
PsuI	RGATCY	4 cut(s)	33, 831, 901, 1070
PvuII	CAGCTG	1 cut(s)	947
RsaI	GTAC	5 cut(s)	407, 614, 881, 966, 1247
RsaNI	GTAC	5 cut(s)	406, 613, 880, 965, 1246
RseI	CAYNNNNRTG	3 cut(s)	563, 743, 1143
SacI	GAGCTC	2 cut(s)	1219, 1278
SapI	GCTCTTC	1 cut(s)	1050
SaqAI	TTAA	3 cut(s)	303, 591, 603
SatI	GCNGC	2 cut(s)	105, 756
Sau96I	GGNCC	4 cut(s)	142, 224, 244, 853
ScrFI	CCNGG	1 cut(s)	668
SduI	GDGCHC	4 cut(s)	1219, 1278, 1320, 1329
SfaNI	GCATC	3 cut(s)	131, 613, 767
SfcI	CTRYAG	1 cut(s)	1222
SinI	GGWCC	2 cut(s)	244, 853
SmiMI	CAYNNNNRTG	3 cut(s)	563, 743, 1143
SmlI	CTYRAG	5 cut(s)	146, 590, 695, 848, 1052
SmoI	CTYRAG	5 cut(s)	146, 590, 695, 848, 1052
Sse9I	AATT	6 cut(s)	396, 689, 894, 969, 1065, 1263
SsiI	CCGC	2 cut(s)	105, 1141
SspMI	CTAG	2 cut(s)	27, 384
SstI	GAGCTC	2 cut(s)	1219, 1278
StyD4I	CCNGG	1 cut(s)	666
StyI	CCWWGG	1 cut(s)	372
TaaI	ACNGT	6 cut(s)	61, 81, 301, 479, 617, 821
TaiI	ACGT	1 cut(s)	753
TaqI	TCGA	5 cut(s)	39, 63, 69, 191, 291
TasI	AATT	6 cut(s)	396, 689, 894, 969, 1065, 1263
TatI	WGTACW	3 cut(s)	405, 964, 1245
TauI	GCSGC	1 cut(s)	107
TfiI	GAWTC	2 cut(s)	523, 1060
Tru1I	TTAA	3 cut(s)	303, 591, 603
Tru9I	TTAA	3 cut(s)	303, 591, 603
TscAI	CASTG	2 cut(s)	1150, 1296
TseFI	GTSAC	2 cut(s)	638, 991
TseI	GCWGC	1 cut(s)	755
Tsp45I	GTSAC	2 cut(s)	638, 991
TspDTI	ATGAA	4 cut(s)	265, 508, 575, 1156
TspGWI	ACGGA	3 cut(s)	1063, 1171, 1301
TspRI	CASTG	2 cut(s)	1150, 1296
Vha464I	CTTAAG	1 cut(s)	590
VpaK11BI	GGWCC	2 cut(s)	244, 853
XagI	CCTNNNNNAGG	1 cut(s)	179
XapI	RAATTY	1 cut(s)	396
XceI	RCATGY	1 cut(s)	491
XspI	CTAG	2 cut(s)	27, 384

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.