Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr1D

Orthogroup ID

OG0002577

Physical Location & Seq

Genomic Coordinates Forward (+)

61382534 .. 61386015

Gene Structure 3482 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh1DG396400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 876 bp

ATGGTTCAACAGCTTGATGGAACTACAAATGAATGGGGTTGGTGCAAACAGAAGCTTGGAGCAAATGCAATATTAGCAGTGTCTCTTGCTGTTTGTAAAGCAGGTGCCATTGTAAAAAAGATACCCCTATACAAGCACATAGCCAATCTTGCTGGAAACAAGACCTTGGTTCTGCCTGTTCCTGCATTTAATGTCATCAATGGAGGTTCTCATGCTGGCAATAAACTAGCCATGCAGGAATTTATGATTCTCCCCGTTGGAGCATCTTCTTTTAAGGAAGCCATGAAGATGGGTGTAGAAGTATATCATCATCTGAAGGCTGTAATTAAGAAGAAGTATGGACAAGATGCCACAAATGTTGGGGATGAAGGTGGCTTTGCACCCAATATTCAGGAAAACAAAGAGGGTCTTGAACTTCTGAAGACAGCTATTGCTAAAGCTGGATATACCGGAAAGGTTGTTATCGGGATGGATGTTGCTGCTTCAGAATTCTATGATGATAAGGATAAGACTTATGATTTGAATTTCAAGGAAGAGAAAAATGACGGATCACAAAGAATATCAGGAGACAGTCTGAAGAATGTTTACAAGTCATTTGTTACAGAGTATCCAATTGTGTCAATTGAAGATCCCTTTGATCAGGATGACTGGGAACACTATGCTAAGATGACTGGTGAAATTGGCGAGCAAGTGCAAATTGTTGGTGATGATCTCCTTGTTACAAACCCAAAGCGAGTGGAGAAAGCAATCAAGGAGAAGAGCTGCAATGCCCTTCTTTTGAAGGTGAATCAAATTGGTTCAGTAACCGAAAGTATTGAAGCTGTGAAAATGTCAAAACGTGCTGGATGGGGTGTCATGGCAAGTCACCGAAGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00010 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko03018 ko04066 map00010 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map03018 map04066

Pfam Domains

Protein Families

Enolase_C Enolase_N

Protein Analysis

291

Amino Acids

31.85

Weight (kDa)

7.66

Isoelectric Point (pI)

38.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Enolase_N	PF03952	2 - 47	3.9e-11	Enolase, N-terminal domain
Enolase_C	PF00113	56 - 290	7.6e-123	Enolase, C-terminal TIM barrel domain
MR_MLE_C	PF13378	182 - 286	2.4e-07	Enolase C-terminal domain-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0002577)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G36530 AT2G36530
fragaria_vesca	FvH4_6g17770 FvH4_6g17770 FvH4_7g28150
malus_domestica	MD01G1185500.v1.1 MD12G1016600.v1.1 MD14G1014600.v1.1
prunus_persica	Prupe.2G281900_v2.0.a1 Prupe.2G281900_v2.0.a1 Prupe.7G118700_v2.0.a1
pyrus_communis	pycom01g19710 pycom12g01360 pycom14g01220
rosa_chinensis	RchiOBHm_Chr1g0375091 RchiOBHm_Chr3g0472001
rosa_laevigata	RLG00000000873 RLG00000024113
rosa_multiflora	Rmu_sc0000090.1_g000028 Rmu_sc0000554.1_g000017 Rmu_sc0002532.1_g000001 Rmu_sc0034562.1_g000001 Rmu_sc0039689.1_g000001
rosa_roxburghii	Rroxscaffold_4G00283020 Rroxscaffold_6G00409370
rosa_rugosa	Rorug01G0388300 Rorug01G0388400 Rorug03G0121500 Rorug03G0121600
rosa_samantha	Rh1AG398800 Rh1BG365700 Rh1CG378600 Rh1DG396400 Rh3AG173200 Rh3BG198600 Rh3CG189800 Rh3CG197100 Rh3DG175800
rosa_wichuraiana	Rw1G035410 Rw3G016750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	92
Acc36I	ACCTGC	1 cut(s)	92
AccB1I	GGYRCC	1 cut(s)	104
AclWI	GGATC	2 cut(s)	556, 623
AcsI	RAATTY	3 cut(s)	239, 488, 523
AcuI	CTGAAG	4 cut(s)	335, 440, 468, 596
AgsI	TTSAA	7 cut(s)	8, 413, 523, 529, 626, 781, 818
AluBI	AGCT	6 cut(s)	13, 55, 428, 440, 762, 821
AluI	AGCT	6 cut(s)	13, 55, 428, 440, 762, 821
Alw26I	GTCTC	2 cut(s)	87, 561
AlwI	GGATC	2 cut(s)	556, 623
ApeKI	GCWGC	2 cut(s)	479, 762
ApoI	RAATTY	3 cut(s)	239, 488, 523
ArsI	GACNNNNNNTTYG	2 cut(s)	502, 534
AsuHPI	GGTGA	4 cut(s)	686, 716, 796, 857
BanI	GGYRCC	1 cut(s)	104
BarI	GAAGNNNNNNTAC	2 cut(s)	569, 601
BbsI	GAAGAC	1 cut(s)	428
BbvI	GCAGC	2 cut(s)	466, 749
BccI	CCATC	4 cut(s)	11, 283, 463, 840
BciVI	GTATCC	1 cut(s)	618
BclI	TGATCA	1 cut(s)	637
BcoDI	GTCTC	2 cut(s)	87, 561
BfaI	CTAG	1 cut(s)	227
BfuAI	ACCTGC	1 cut(s)	92
BfuI	GTATCC	1 cut(s)	618
BisI	GCNGC	2 cut(s)	480, 763
BlsI	GCNGC	2 cut(s)	481, 764
BmiI	GGNNCC	1 cut(s)	106
BmrI	ACTGGG	1 cut(s)	658
BmsI	GCATC	2 cut(s)	272, 337
BmuI	ACTGGG	1 cut(s)	658
BpiI	GAAGAC	1 cut(s)	428
BsaJI	CCNNGG	1 cut(s)	165
BsaWI	WCCGGW	1 cut(s)	449
Bse1I	ACTGG	2 cut(s)	653, 676
Bse3DI	GCAATG	1 cut(s)	772
BseDI	CCNNGG	1 cut(s)	165
BseGI	GGATG	5 cut(s)	370, 474, 478, 649, 851
BseMI	GCAATG	1 cut(s)	772
BseNI	ACTGG	2 cut(s)	653, 676
BseXI	GCAGC	2 cut(s)	466, 749
BshNI	GGYRCC	1 cut(s)	104
BsiSI	CCGG	1 cut(s)	450
BsmAI	GTCTC	2 cut(s)	87, 561
Bsp143I	GATC	4 cut(s)	548, 628, 637, 709
BspLI	GGNNCC	1 cut(s)	106
BspMI	ACCTGC	1 cut(s)	92
BspPI	GGATC	2 cut(s)	556, 623
BspQI	GCTCTTC	1 cut(s)	752
BspT107I	GGYRCC	1 cut(s)	104
BsrDI	GCAATG	1 cut(s)	772
BsrI	ACTGG	2 cut(s)	653, 676
BssECI	CCNNGG	1 cut(s)	165
BssMI	GATC	4 cut(s)	548, 628, 637, 709
BssT1I	CCWWGG	1 cut(s)	165
Bst4CI	ACNGT	1 cut(s)	572
Bst6I	CTCTTC	2 cut(s)	528, 752
BstC8I	GCNNGC	2 cut(s)	217, 686
BstDEI	CTNAG	1 cut(s)	663
BstF5I	GGATG	5 cut(s)	370, 474, 478, 649, 851
BstKTI	GATC	4 cut(s)	551, 631, 640, 712
BstMAI	GTCTC	2 cut(s)	87, 561
BstMBI	GATC	4 cut(s)	548, 628, 637, 709
BstMWI	GCNNNNNNNGC	2 cut(s)	74, 149
BstV1I	GCAGC	2 cut(s)	466, 749
BstV2I	GAAGAC	1 cut(s)	428
BstX2I	RGATCY	1 cut(s)	628
BstXI	CCANNNNNNTGG	1 cut(s)	289
BstYI	RGATCY	1 cut(s)	628
BsuI	GTATCC	1 cut(s)	618
BtsCI	GGATG	5 cut(s)	370, 474, 478, 649, 851
BtsI	GCAGTG	1 cut(s)	84
BtsIMutI	CAGTG	1 cut(s)	84
BveI	ACCTGC	1 cut(s)	92
Cac8I	GCNNGC	2 cut(s)	217, 686
CspCI	CAANNNNNGTGG	4 cut(s)	340, 375, 717, 752
CviAII	CATG	4 cut(s)	212, 232, 283, 856
DdeI	CTNAG	1 cut(s)	663
DpnI	GATC	4 cut(s)	550, 630, 639, 711
DpnII	GATC	4 cut(s)	548, 628, 637, 709
Eam1104I	CTCTTC	2 cut(s)	528, 752
EarI	CTCTTC	2 cut(s)	528, 752
Eco130I	CCWWGG	1 cut(s)	165
Eco57I	CTGAAG	4 cut(s)	335, 440, 468, 596
EcoRI	GAATTC	1 cut(s)	488
EcoT14I	CCWWGG	1 cut(s)	165
ErhI	CCWWGG	1 cut(s)	165
FaeI	CATG	4 cut(s)	215, 235, 286, 859
FalI	AAGNNNNNCTT	2 cut(s)	393, 425
FatI	CATG	4 cut(s)	211, 231, 282, 855
FbaI	TGATCA	1 cut(s)	637
Fnu4HI	GCNGC	2 cut(s)	480, 763
FokI	GGATG	5 cut(s)	377, 481, 485, 656, 858
Fsp4HI	GCNGC	2 cut(s)	480, 763
FspBI	CTAG	1 cut(s)	227
GluI	GCNGC	2 cut(s)	480, 763
HapII	CCGG	1 cut(s)	450
Hin1II	CATG	4 cut(s)	215, 235, 286, 859
HindIII	AAGCTT	1 cut(s)	53
HinfI	GANTC	2 cut(s)	247, 787
HpaII	CCGG	1 cut(s)	450
HphI	GGTGA	4 cut(s)	686, 716, 796, 857
Hpy166II	GTNNAC	1 cut(s)	586
Hpy188I	TCNGA	4 cut(s)	315, 420, 487, 576
Hpy188III	TCNNGA	5 cut(s)	392, 410, 466, 564, 641
Hpy8I	GTNNAC	1 cut(s)	586
HpyAV	CCTTC	5 cut(s)	310, 362, 775, 782, 864
HpyCH4III	ACNGT	1 cut(s)	572
HpyCH4IV	ACGT	1 cut(s)	838
HpyCH4V	TGCA	7 cut(s)	45, 68, 185, 235, 380, 694, 765
HpyF10VI	GCNNNNNNNGC	2 cut(s)	74, 149
HpyF3I	CTNAG	1 cut(s)	663
HpySE526I	ACGT	1 cut(s)	838
Hsp92II	CATG	4 cut(s)	215, 235, 286, 859
Ksp22I	TGATCA	1 cut(s)	637
Kzo9I	GATC	4 cut(s)	548, 628, 637, 709
LguI	GCTCTTC	1 cut(s)	752
LmnI	GCTCC	2 cut(s)	59, 260
Lsp1109I	GCAGC	2 cut(s)	466, 749
LweI	GCATC	2 cut(s)	272, 337
MaeI	CTAG	1 cut(s)	227
MaeII	ACGT	1 cut(s)	838
MaeIII	GTNAC	4 cut(s)	598, 718, 802, 863
MalI	GATC	4 cut(s)	550, 630, 639, 711
MboI	GATC	4 cut(s)	548, 628, 637, 709
MboII	GAAGA	8 cut(s)	258, 298, 343, 433, 545, 589, 638, 769
MfeI	CAATTG	2 cut(s)	612, 621
MflI	RGATCY	1 cut(s)	628
MluCI	AATT	9 cut(s)	239, 324, 488, 523, 612, 621, 678, 696, 792
MmeI	TCCRAC	1 cut(s)	238
MnlI	CCTC	2 cut(s)	197, 397
MseI	TTAA	3 cut(s)	189, 273, 327
MslI	CAYNNNNRTG	1 cut(s)	287
MspI	CCGG	1 cut(s)	450
MunI	CAATTG	2 cut(s)	612, 621
MwoI	GCNNNNNNNGC	2 cut(s)	74, 149
NdeII	GATC	4 cut(s)	548, 628, 637, 709
NlaIII	CATG	4 cut(s)	215, 235, 286, 859
NlaIV	GGNNCC	1 cut(s)	106
NmuCI	GTSAC	1 cut(s)	863
PaqCI	CACCTGC	1 cut(s)	92
PciSI	GCTCTTC	1 cut(s)	752
PfeI	GAWTC	2 cut(s)	247, 787
PkrI	GCNGC	2 cut(s)	481, 764
PspN4I	GGNNCC	1 cut(s)	106
PsuI	RGATCY	1 cut(s)	628
RseI	CAYNNNNRTG	1 cut(s)	287
SapI	GCTCTTC	1 cut(s)	752
SaqAI	TTAA	3 cut(s)	189, 273, 327
SatI	GCNGC	2 cut(s)	480, 763
Sau3AI	GATC	4 cut(s)	548, 628, 637, 709
SfaNI	GCATC	2 cut(s)	272, 337
SmiMI	CAYNNNNRTG	1 cut(s)	287
Sse9I	AATT	9 cut(s)	239, 324, 488, 523, 612, 621, 678, 696, 792
SspI	AATATT	2 cut(s)	72, 388
SspMI	CTAG	1 cut(s)	227
StyI	CCWWGG	1 cut(s)	165
TaaI	ACNGT	1 cut(s)	572
TaiI	ACGT	1 cut(s)	841
TasI	AATT	9 cut(s)	239, 324, 488, 523, 612, 621, 678, 696, 792
TfiI	GAWTC	2 cut(s)	247, 787
Tru1I	TTAA	3 cut(s)	189, 273, 327
Tru9I	TTAA	3 cut(s)	189, 273, 327
TscAI	CASTG	1 cut(s)	84
TseFI	GTSAC	1 cut(s)	863
TseI	GCWGC	2 cut(s)	479, 762
Tsp45I	GTSAC	1 cut(s)	863
TspDTI	ATGAA	3 cut(s)	45, 299, 381
TspGWI	ACGGA	1 cut(s)	561
TspRI	CASTG	1 cut(s)	84
XapI	RAATTY	3 cut(s)	239, 488, 523
XspI	CTAG	1 cut(s)	227

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.