Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000006

Orthogroup ID

OG0002577

Physical Location & Seq

Genomic Coordinates Reverse (-)

31880430 .. 31885156

Gene Structure 4727 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_6G00409370.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1335 bp

ATGGCTACGATCCAGTCAGTTAAGGCGAGGCAGATCTTCGACAGCCGTGGAAATCCCACCGTCGAGGTCGATATAGTACTTTCAGATGGTTTTTTGGCTAGAGCTGCTGTTCCCAGTGGTGCCTCTACCGGTGTGTACGAGGCTCTTGAGTTGCGTGATGGAGGCAAAGACTACCTTGGAAAGGGTGTGTCGAAGGCTGTCAACAATGTCAACTCAATCATCGGCCCTGCTTTGATTGGCAAGGACCCATCTGAGCAGACTGCCATTGATAACTTTATGGTTCAGCAGCTCGATGGAACTGTTAATGAGTGGGGTTGGTGTAAACAAAAGCTTGGTGCAAATGCCATACTGGCCGTGTCTCTTGCAGTCTGCAAAGCTGGGGCTAGTGTTAAGAAAATTCCTCTTTACAAGCACATTGCCAATCTTGCTTGCAACAAGAACTTGGTGTTGCCTGTTCCTGCTTTCAATGTCATCAATGGAGGATCACATGCAGGAAACAAACTTGCCATGCAGGAGTTCATGATTCTTCCTGTTGGAGCTTCCTCATTCAAAGAGGCCATGAAGATGGGTGTGGAAGTATATCATAATCTAAAGTCTGTGATTAAGAAGAAATACGGTCAGGATGCAACCAACGTTGGTGACGAAGGTGGATTTGCTCCCAACATTCAGGAGAACAAGGAAGGTCTTGAATTGCTTAAGACTGCCATTGAGAAGGCTGGATACACTGGAAAGGTTGTCATTGGAATGGATGTTGCTGCATCTGAGTTTTATGGAACAGATAAAACCTATGACCTGAACTTCAAGGAAGAGAAAAATGATGGTTCCCAGAAGATTTCCGGAAATGCTCTCAAGGATCTCTACAAGTCATTTGCATCCGAGTACCCTATTGTCTCGATTGAAGATCCCTTTGACCAAGATGATTGGGAGCACTACTCCAAGATGACTGCTGAATGTGGAGAGCAAGTACAAATTGTCGGAGATGATCTGTTGGTCACCAACCCCAAGAGGGTTGAGAAAGCAATCAAGGAGAAAAGTTGCAATGCTCTTCTTCTGAAGGTGAATCAAATTGGGTCCGTAACTGAGAGTATTGAGGCCGTAAGAATGTCTAAGAAAGCTGGGTGGGGTGTCATGGCCAGCCACAGAAGTGGAGAAACTGAGGACACTTTCATTGCTGATCTTTCCGTCGGTTTGGCCACGGGTCAAATCAAGACCGGTGCTCCCTGCAGGTCAGAGCGTCTTGCCAAGTATAACCAGCTCTTGCGTATTGAGGAGGAACTTGGTTCAGCAGCAGTCTATGCCGGAGCAAAGTTCCGTGTGCCAGTCGAACCCTATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004634 GO:0005488 GO:0005507 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005740 GO:0005829 GO:0005886 GO:0005911 GO:0006950 GO:0006970 GO:0008150 GO:0009266 GO:0009314 GO:0009409 GO:0009416 GO:0009506 GO:0009507 GO:0009536 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0010033 GO:0010035 GO:0010038 GO:0016020 GO:0016829 GO:0016835 GO:0016836 GO:0030054 GO:0031967 GO:0031975 GO:0033993 GO:0042221 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0046686 GO:0046872 GO:0046914 GO:0048046 GO:0050896 GO:0055044 GO:0071944 GO:0097159 GO:0097305 GO:1901363 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00010 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko03018 ko04066 map00010 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map03018 map04066

Pfam Domains

Protein Families

Enolase_C Enolase_N

Protein Analysis

444

Amino Acids

47.87

Weight (kDa)

5.91

Isoelectric Point (pI)

37.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Enolase_N	PF03952	4 - 139	5.6e-53	Enolase, N-terminal domain
Enolase_C	PF00113	148 - 438	1.8e-158	Enolase, C-terminal TIM barrel domain
MR_MLE_C	PF13378	279 - 405	5e-08	Enolase C-terminal domain-like
MAAL_C	PF07476	297 - 401	4.6e-06	Methylaspartate ammonia-lyase C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002577)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G36530 AT2G36530
fragaria_vesca	FvH4_6g17770 FvH4_6g17770 FvH4_7g28150
malus_domestica	MD01G1185500.v1.1 MD12G1016600.v1.1 MD14G1014600.v1.1
prunus_persica	Prupe.2G281900_v2.0.a1 Prupe.2G281900_v2.0.a1 Prupe.7G118700_v2.0.a1
pyrus_communis	pycom01g19710 pycom12g01360 pycom14g01220
rosa_chinensis	RchiOBHm_Chr1g0375091 RchiOBHm_Chr3g0472001
rosa_laevigata	RLG00000000873 RLG00000024113
rosa_multiflora	Rmu_sc0000090.1_g000028 Rmu_sc0000554.1_g000017 Rmu_sc0002532.1_g000001 Rmu_sc0034562.1_g000001 Rmu_sc0039689.1_g000001
rosa_roxburghii	Rroxscaffold_4G00283020 Rroxscaffold_6G00409370
rosa_rugosa	Rorug01G0388300 Rorug01G0388400 Rorug03G0121500 Rorug03G0121600
rosa_samantha	Rh1AG398800 Rh1BG365700 Rh1CG378600 Rh1DG396400 Rh3AG173200 Rh3BG198600 Rh3CG189800 Rh3CG197100 Rh3DG175800
rosa_wichuraiana	Rw1G035410 Rw3G016750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1215
AccB1I	GGYRCC	1 cut(s)	119
AccIII	TCCGGA	1 cut(s)	836
AclI	AACGTT	1 cut(s)	633
AclWI	GGATC	4 cut(s)	4, 490, 861, 896
AcoI	YGGCCR	3 cut(s)	351, 1131, 1191
AcsI	RAATTY	1 cut(s)	396
AcuI	CTGAAG	1 cut(s)	1073
AfaI	GTAC	4 cut(s)	78, 137, 881, 966
AfiI	CCNNNNNNNGG	2 cut(s)	181, 1006
AflII	CTTAAG	1 cut(s)	695
AgeI	ACCGGT	2 cut(s)	128, 1211
AgsI	TTSAA	5 cut(s)	466, 550, 689, 802, 899
AleI	CACNNNNGTG	1 cut(s)	1143
AluBI	AGCT	7 cut(s)	104, 289, 331, 377, 539, 1115, 1255
AluI	AGCT	7 cut(s)	104, 289, 331, 377, 539, 1115, 1255
Alw21I	GWGCWC	2 cut(s)	930, 1219
Alw26I	GTCTC	2 cut(s)	363, 895
AlwI	GGATC	4 cut(s)	4, 490, 861, 896
Aor13HI	TCCGGA	1 cut(s)	836
AoxI	GGCC	6 cut(s)	223, 351, 555, 1092, 1131, 1191
ApeKI	GCWGC	4 cut(s)	104, 286, 755, 1286
ApoI	RAATTY	1 cut(s)	396
AsiGI	ACCGGT	2 cut(s)	128, 1211
AspS9I	GGNCC	3 cut(s)	224, 244, 1071
AsuHPI	GGTGA	3 cut(s)	650, 985, 1069
AvaII	GGWCC	2 cut(s)	244, 1071
BalI	TGGCCA	2 cut(s)	1133, 1193
BanI	GGYRCC	1 cut(s)	119
BarI	GAAGNNNNNNTAC	2 cut(s)	704, 736
Bbv12I	GWGCWC	2 cut(s)	930, 1219
BbvI	GCAGC	4 cut(s)	91, 298, 742, 1298
BccI	CCATC	6 cut(s)	80, 152, 256, 287, 559, 812
BceAI	ACGGC	3 cut(s)	30, 338, 1079
BciVI	GTATCC	1 cut(s)	713
BcoDI	GTCTC	2 cut(s)	363, 895
BfaI	CTAG	2 cut(s)	99, 384
BfmI	CTRYAG	1 cut(s)	1222
BfrI	CTTAAG	1 cut(s)	695
BfuAI	ACCTGC	1 cut(s)	1215
BfuI	GTATCC	1 cut(s)	713
BglI	GCCNNNNNGGC	1 cut(s)	350
BglII	AGATCT	1 cut(s)	33
BisI	GCNGC	4 cut(s)	105, 287, 756, 1287
BlsI	GCNGC	4 cut(s)	106, 288, 757, 1288
BmcAI	AGTACT	1 cut(s)	78
Bme18I	GGWCC	2 cut(s)	244, 1071
BmgT120I	GGNCC	3 cut(s)	224, 244, 1071
BmiI	GGNNCC	4 cut(s)	121, 246, 823, 1072
BmrI	ACTGGG	1 cut(s)	108
BmsI	GCATC	3 cut(s)	613, 767, 881
BmuI	ACTGGG	1 cut(s)	108
BplI	GAGNNNNNCTC	2 cut(s)	917, 949
BpuEI	CTTGAG	2 cut(s)	167, 833
BsaJI	CCNNGG	3 cut(s)	46, 175, 1194
BsaWI	WCCGGW	3 cut(s)	128, 836, 1211
BsaXI	ACNNNNNCTCC	4 cut(s)	948, 978, 1201, 1231
Bsc4I	CCNNNNNNNGG	2 cut(s)	181, 1006
Bse118I	RCCGGY	2 cut(s)	128, 1211
Bse1I	ACTGG	5 cut(s)	13, 114, 354, 730, 1319
Bse3DI	GCAATG	3 cut(s)	414, 1045, 1167
BseAI	TCCGGA	1 cut(s)	836
BseDI	CCNNGG	3 cut(s)	46, 175, 1194
BseGI	GGATG	3 cut(s)	628, 754, 872
BseLI	CCNNNNNNNGG	2 cut(s)	181, 1006
BseMI	GCAATG	3 cut(s)	414, 1045, 1167
BseMII	CTCAG	4 cut(s)	243, 753, 1071, 1146
BseNI	ACTGG	5 cut(s)	13, 114, 354, 730, 1319
BseRI	GAGGAG	1 cut(s)	1283
BseXI	GCAGC	4 cut(s)	91, 298, 742, 1298
BseYI	CCCAGC	2 cut(s)	377, 1115
BshFI	GGCC	6 cut(s)	225, 353, 557, 1094, 1133, 1193
BshNI	GGYRCC	1 cut(s)	119
BshTI	ACCGGT	2 cut(s)	128, 1211
BsiHKAI	GWGCWC	2 cut(s)	930, 1219
BsiSI	CCGG	4 cut(s)	129, 837, 1212, 1299
BslI	CCNNNNNNNGG	2 cut(s)	181, 1006
BsmAI	GTCTC	2 cut(s)	363, 895
BsnI	GGCC	6 cut(s)	225, 353, 557, 1094, 1133, 1193
Bsp1286I	GDGCHC	2 cut(s)	930, 1219
Bsp13I	TCCGGA	1 cut(s)	836
Bsp143I	GATC	7 cut(s)	9, 33, 482, 853, 901, 982, 1174
BspANI	GGCC	6 cut(s)	225, 353, 557, 1094, 1133, 1193
BspCNI	CTCAG	4 cut(s)	244, 754, 1072, 1147
BspEI	TCCGGA	1 cut(s)	836
BspHI	TCATGA	1 cut(s)	519
BspLI	GGNNCC	4 cut(s)	121, 246, 823, 1072
BspMAI	CTGCAG	1 cut(s)	1226
BspMI	ACCTGC	1 cut(s)	1215
BspPI	GGATC	4 cut(s)	4, 490, 861, 896
BspQI	GCTCTTC	1 cut(s)	1050
BspT107I	GGYRCC	1 cut(s)	119
BspTI	CTTAAG	1 cut(s)	695
BsrDI	GCAATG	3 cut(s)	414, 1045, 1167
BsrFI	RCCGGY	2 cut(s)	128, 1211
BsrI	ACTGG	5 cut(s)	13, 114, 354, 730, 1319
BssAI	RCCGGY	2 cut(s)	128, 1211
BssECI	CCNNGG	3 cut(s)	46, 175, 1194
BssMI	GATC	7 cut(s)	9, 33, 482, 853, 901, 982, 1174
BssT1I	CCWWGG	1 cut(s)	175
Bst4CI	ACNGT	3 cut(s)	61, 301, 617
Bst6I	CTCTTC	2 cut(s)	801, 1050
BstAFI	CTTAAG	1 cut(s)	695
BstAPI	GCANNNNNTGC	1 cut(s)	1295
BstC8I	GCNNGC	2 cut(s)	430, 1135
BstDEI	CTNAG	5 cut(s)	252, 762, 1080, 1107, 1155
BstDSI	CCRYGG	2 cut(s)	46, 1194
BstEII	GGTNACC	1 cut(s)	991
BstENI	CCTNNNNNAGG	1 cut(s)	179
BstF5I	GGATG	3 cut(s)	628, 754, 872
BstKTI	GATC	7 cut(s)	12, 36, 485, 856, 904, 985, 1177
BstMAI	GTCTC	2 cut(s)	363, 895
BstMBI	GATC	7 cut(s)	9, 33, 482, 853, 901, 982, 1174
BstMWI	GCNNNNNNNGC	4 cut(s)	104, 350, 425, 1295
BstNSI	RCATGY	1 cut(s)	491
BstPI	GGTNACC	1 cut(s)	991
BstSFI	CTRYAG	1 cut(s)	1222
BstV1I	GCAGC	4 cut(s)	91, 298, 742, 1298
BstX2I	RGATCY	3 cut(s)	33, 853, 901
BstXI	CCANNNNNNTGG	2 cut(s)	565, 1145
BstYI	RGATCY	3 cut(s)	33, 853, 901
BsuI	GTATCC	1 cut(s)	713
BsuRI	GGCC	6 cut(s)	225, 353, 557, 1094, 1133, 1193
BtgI	CCRYGG	2 cut(s)	46, 1194
BtsCI	GGATG	3 cut(s)	628, 754, 872
BtsIMutI	CAGTG	2 cut(s)	121, 723
BveI	ACCTGC	1 cut(s)	1215
Cac8I	GCNNGC	2 cut(s)	430, 1135
CciI	TCATGA	1 cut(s)	519
Cfr10I	RCCGGY	2 cut(s)	128, 1211
Cfr13I	GGNCC	3 cut(s)	224, 244, 1071
CseI	GACGC	1 cut(s)	1223
Csp6I	GTAC	4 cut(s)	77, 136, 880, 965
CspAI	ACCGGT	2 cut(s)	128, 1211
CviAII	CATG	5 cut(s)	488, 508, 520, 559, 1129
CviQI	GTAC	4 cut(s)	77, 136, 880, 965
DdeI	CTNAG	5 cut(s)	252, 762, 1080, 1107, 1155
DpnI	GATC	7 cut(s)	11, 35, 484, 855, 903, 984, 1176
DpnII	GATC	7 cut(s)	9, 33, 482, 853, 901, 982, 1174
EaeI	YGGCCR	3 cut(s)	351, 1131, 1191
Eam1104I	CTCTTC	2 cut(s)	801, 1050
EarI	CTCTTC	2 cut(s)	801, 1050
Eco130I	CCWWGG	1 cut(s)	175
Eco47I	GGWCC	2 cut(s)	244, 1071
Eco57I	CTGAAG	1 cut(s)	1073
Eco91I	GGTNACC	1 cut(s)	991
EcoNI	CCTNNNNNAGG	1 cut(s)	179
EcoO109I	RGGNCCY	1 cut(s)	244
EcoO65I	GGTNACC	1 cut(s)	991
EcoT14I	CCWWGG	1 cut(s)	175
ErhI	CCWWGG	1 cut(s)	175
FaeI	CATG	5 cut(s)	491, 511, 523, 562, 1132
FalI	AAGNNNNNCTT	2 cut(s)	159, 191
FatI	CATG	5 cut(s)	487, 507, 519, 558, 1128
Fnu4HI	GCNGC	4 cut(s)	105, 287, 756, 1287
FokI	GGATG	3 cut(s)	635, 761, 859
Fsp4HI	GCNGC	4 cut(s)	105, 287, 756, 1287
FspBI	CTAG	2 cut(s)	99, 384
GluI	GCNGC	4 cut(s)	105, 287, 756, 1287
GsaI	CCCAGC	2 cut(s)	381, 1119
HaeIII	GGCC	6 cut(s)	225, 353, 557, 1094, 1133, 1193
HapII	CCGG	4 cut(s)	129, 837, 1212, 1299
HgaI	GACGC	1 cut(s)	1223
Hin1II	CATG	5 cut(s)	491, 511, 523, 562, 1132
HincII	GTYRAC	2 cut(s)	202, 211
HindII	GTYRAC	2 cut(s)	202, 211
HindIII	AAGCTT	1 cut(s)	329
HinfI	GANTC	2 cut(s)	523, 1060
HpaII	CCGG	4 cut(s)	129, 837, 1212, 1299
HphI	GGTGA	3 cut(s)	650, 985, 1069
Hpy166II	GTNNAC	4 cut(s)	136, 202, 211, 323
Hpy188I	TCNGA	7 cut(s)	85, 253, 763, 877, 977, 1053, 1231
Hpy188III	TCNNGA	8 cut(s)	146, 520, 620, 668, 686, 837, 892, 1207
Hpy8I	GTNNAC	4 cut(s)	136, 202, 211, 323
Hpy99I	CGWCG	2 cut(s)	65, 1187
HpyAV	CCTTC	5 cut(s)	187, 638, 674, 706, 1048
HpyCH4III	ACNGT	3 cut(s)	61, 301, 617
HpyCH4IV	ACGT	1 cut(s)	633
HpyF10VI	GCNNNNNNNGC	4 cut(s)	104, 350, 425, 1295
HpyF3I	CTNAG	5 cut(s)	252, 762, 1080, 1107, 1155
HpySE526I	ACGT	1 cut(s)	633
Hsp92II	CATG	5 cut(s)	491, 511, 523, 562, 1132
Kpn2I	TCCGGA	1 cut(s)	836
Kzo9I	GATC	7 cut(s)	9, 33, 482, 853, 901, 982, 1174
LguI	GCTCTTC	1 cut(s)	1050
LmnI	GCTCC	5 cut(s)	536, 661, 925, 1222, 1301
Lsp1109I	GCAGC	4 cut(s)	91, 298, 742, 1298
LweI	GCATC	3 cut(s)	613, 767, 881
MaeI	CTAG	2 cut(s)	99, 384
MaeII	ACGT	1 cut(s)	633
MaeIII	GTNAC	3 cut(s)	638, 991, 1075
MalI	GATC	7 cut(s)	11, 35, 484, 855, 903, 984, 1176
MboI	GATC	7 cut(s)	9, 33, 482, 853, 901, 982, 1174
MboII	GAAGA	9 cut(s)	28, 518, 574, 619, 818, 841, 911, 1037, 1040
MflI	RGATCY	3 cut(s)	33, 853, 901
MhlI	GDGCHC	2 cut(s)	930, 1219
MlsI	TGGCCA	2 cut(s)	1133, 1193
MluCI	AATT	4 cut(s)	396, 689, 969, 1065
MluNI	TGGCCA	2 cut(s)	1133, 1193
MmeI	TCCRAC	2 cut(s)	514, 955
Mox20I	TGGCCA	2 cut(s)	1133, 1193
MroI	TCCGGA	1 cut(s)	836
MscI	TGGCCA	2 cut(s)	1133, 1193
MseI	TTAA	5 cut(s)	21, 303, 390, 603, 696
MslI	CAYNNNNRTG	3 cut(s)	563, 743, 1143
Msp20I	TGGCCA	2 cut(s)	1133, 1193
MspCI	CTTAAG	1 cut(s)	695
MspI	CCGG	4 cut(s)	129, 837, 1212, 1299
MwoI	GCNNNNNNNGC	4 cut(s)	104, 350, 425, 1295
NdeII	GATC	7 cut(s)	9, 33, 482, 853, 901, 982, 1174
NlaIII	CATG	5 cut(s)	491, 511, 523, 562, 1132
NlaIV	GGNNCC	4 cut(s)	121, 246, 823, 1072
NmuCI	GTSAC	2 cut(s)	638, 991
NspI	RCATGY	1 cut(s)	491
OliI	CACNNNNGTG	1 cut(s)	1143
PagI	TCATGA	1 cut(s)	519
PciSI	GCTCTTC	1 cut(s)	1050
PcsI	WCGNNNNNNNCGW	1 cut(s)	639
PfeI	GAWTC	2 cut(s)	523, 1060
PinAI	ACCGGT	2 cut(s)	128, 1211
PkrI	GCNGC	4 cut(s)	106, 288, 757, 1288
PpuMI	RGGWCCY	1 cut(s)	244
Psp1406I	AACGTT	1 cut(s)	633
Psp5II	RGGWCCY	1 cut(s)	244
PspEI	GGTNACC	1 cut(s)	991
PspFI	CCCAGC	2 cut(s)	377, 1115
PspN4I	GGNNCC	4 cut(s)	121, 246, 823, 1072
PspPI	GGNCC	3 cut(s)	224, 244, 1071
PspPPI	RGGWCCY	1 cut(s)	244
PstI	CTGCAG	1 cut(s)	1226
PsuI	RGATCY	3 cut(s)	33, 853, 901
RsaI	GTAC	4 cut(s)	78, 137, 881, 966
RsaNI	GTAC	4 cut(s)	77, 136, 880, 965
RseI	CAYNNNNRTG	3 cut(s)	563, 743, 1143
SapI	GCTCTTC	1 cut(s)	1050
SaqAI	TTAA	5 cut(s)	21, 303, 390, 603, 696
SatI	GCNGC	4 cut(s)	105, 287, 756, 1287
Sau3AI	GATC	7 cut(s)	9, 33, 482, 853, 901, 982, 1174
Sau96I	GGNCC	3 cut(s)	224, 244, 1071
SbfI	CCTGCAGG	1 cut(s)	1226
ScaI	AGTACT	1 cut(s)	78
SdaI	CCTGCAGG	1 cut(s)	1226
SduI	GDGCHC	2 cut(s)	930, 1219
SfaNI	GCATC	3 cut(s)	613, 767, 881
SfcI	CTRYAG	1 cut(s)	1222
SinI	GGWCC	2 cut(s)	244, 1071
SmiMI	CAYNNNNRTG	3 cut(s)	563, 743, 1143
SmlI	CTYRAG	3 cut(s)	146, 695, 848
SmoI	CTYRAG	3 cut(s)	146, 695, 848
Sse8387I	CCTGCAGG	1 cut(s)	1226
Sse9I	AATT	4 cut(s)	396, 689, 969, 1065
SspMI	CTAG	2 cut(s)	99, 384
StyI	CCWWGG	1 cut(s)	175
TaaI	ACNGT	3 cut(s)	61, 301, 617
TaiI	ACGT	1 cut(s)	636
TaqI	TCGA	7 cut(s)	39, 63, 69, 191, 291, 893, 1323
TasI	AATT	4 cut(s)	396, 689, 969, 1065
TatI	WGTACW	2 cut(s)	76, 964
TfiI	GAWTC	2 cut(s)	523, 1060
Tru1I	TTAA	5 cut(s)	21, 303, 390, 603, 696
Tru9I	TTAA	5 cut(s)	21, 303, 390, 603, 696
TscAI	CASTG	2 cut(s)	121, 730
TseFI	GTSAC	2 cut(s)	638, 991
TseI	GCWGC	4 cut(s)	104, 286, 755, 1286
Tsp45I	GTSAC	2 cut(s)	638, 991
TspDTI	ATGAA	3 cut(s)	508, 575, 1156
TspGWI	ACGGA	3 cut(s)	1063, 1171, 1301
TspRI	CASTG	2 cut(s)	121, 730
Vha464I	CTTAAG	1 cut(s)	695
VpaK11BI	GGWCC	2 cut(s)	244, 1071
XagI	CCTNNNNNAGG	1 cut(s)	179
XapI	RAATTY	1 cut(s)	396
XceI	RCATGY	1 cut(s)	491
XspI	CTAG	2 cut(s)	99, 384
ZrmI	AGTACT	1 cut(s)	78

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.