Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0002577

Physical Location & Seq

Genomic Coordinates Reverse (-)

28454060 .. 28459181

Gene Structure 5122 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1185500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1338 bp

ATGGCCACGATCAAGCTTGTTAAAGCCCGTCAAATCTTCGACAGCCGTGGGAATCCCACCGTCGAAGTTGATGTTACACTGTCGGATGGAACCTACGCAAGAGCTGCTGTTCCAAGTGGGGCTTCCACAGGTATCTATGAAGCCCTTGAACTGAGAGATGGCGGATCGAATTATCTTGGAAAGGGTGTCCTCAAGGCTGTGGAGAATGTGAATTCGATCATTGGACCAGCTTTGATTGGAAAGGACCCAACAGAGCAGACCAAAATCGACAATTATATGGTACAGCAACTCGATGGAACTGTTAACGAATGGGGTTGGTGCAAACAGAAGCTCGGAGCAAATGCTATATTGGCGGTGTCTCTAGCTGTTTGCAAAGCCGGTGCCCTTGTGAACAAGATCCCTCTTTACAAGCATATAGCCAATCTTGCTGGAAACAAGACCTTGGTGCTGCCCGTACCTGCGTTTAATGTCATTAACGGAGGTTCTCATGCAGGCAATAAACTAGCAATGCAGGAATTCATGATCCTCCCTGTTGGGGCATCTTGTTTCAAGGAAGCGATGAAGATGGGTGTGGAAGTATATCATCACCTGAAGGCTGTAATTAAGAAGAAGTACGGACAAGATGCCACGAATGTTGGGGATGAAGGTGGCTTTGCACCCAATATTCAGGAAAACAAAGAGGGTCTTGAACTGCTAAAGACGGCCATAGCTCAAGCTGGATATACTGGAAAGGTTGTCATTGGAATGGATGTTGCTGCCTCAGAATTCTACGATAACAAGGATAAGACCTATGACTTGAACTTCAAGGAAGAGAAAAATGATGGATCACAAAAGATATCTGGAGACAGTCTGAAGAATGTTTACAAGTCATTTGTTGCTGAGTATCCAATTGTGTCAATTGAAGATCCGTTTGACCAGGATGATTGGGAACACTATGCAAAGATGACTGCTGAAATTGGTGAGCAAGTCCAAATTGTCGGCGATGATCTTCTCGTCACAAATCCAAAGCGAGTAAAGAAAGCAATCAATGAGAAGTCCTGCAATGCCCTTCTTTTGAAGGTGAATCAAATTGGTTCGGTTACCGAAAGTATTGAAGCTGTGAAAATGTCAAAGCGGGCTGGCTGGGGTGTCATGGCAAGTCACCGAAGCGGTGAAACTGAGGATACTTTCATTGCCGACCTTGCTGTTGGGCTGTCAACGGGACAGATTAAGACCGGAGCTCCGTGCAGATCAGAGCGGCTTGCTAAATACAACCAGCTTCTTAGGATCGAGGAGGAGCTTGGATCCGCAGCAATCTATGCCGGTGCAAAATTCAGGGTCCCAGTGGAACCGTACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00010 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko03018 ko04066 map00010 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map03018 map04066

Pfam Domains

Protein Families

Enolase_C Enolase_N

Protein Analysis

446

Amino Acids

48.11

Weight (kDa)

6.16

Isoelectric Point (pI)

38.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Enolase_N	PF03952	4 - 139	7.2e-53	Enolase, N-terminal domain
Enolase_C	PF00113	148 - 439	6e-159	Enolase, C-terminal TIM barrel domain
MR_MLE_C	PF13378	276 - 402	2.4e-08	Enolase C-terminal domain-like
MAAL_C	PF07476	298 - 401	9.3e-06	Methylaspartate ammonia-lyase C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002577)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G36530 AT2G36530
fragaria_vesca	FvH4_6g17770 FvH4_6g17770 FvH4_7g28150
malus_domestica	MD01G1185500.v1.1 MD12G1016600.v1.1 MD14G1014600.v1.1
prunus_persica	Prupe.2G281900_v2.0.a1 Prupe.2G281900_v2.0.a1 Prupe.7G118700_v2.0.a1
pyrus_communis	pycom01g19710 pycom12g01360 pycom14g01220
rosa_chinensis	RchiOBHm_Chr1g0375091 RchiOBHm_Chr3g0472001
rosa_laevigata	RLG00000000873 RLG00000024113
rosa_multiflora	Rmu_sc0000090.1_g000028 Rmu_sc0000554.1_g000017 Rmu_sc0002532.1_g000001 Rmu_sc0034562.1_g000001 Rmu_sc0039689.1_g000001
rosa_roxburghii	Rroxscaffold_4G00283020 Rroxscaffold_6G00409370
rosa_rugosa	Rorug01G0388300 Rorug01G0388400 Rorug03G0121500 Rorug03G0121600
rosa_samantha	Rh1AG398800 Rh1BG365700 Rh1CG378600 Rh1DG396400 Rh3AG173200 Rh3BG198600 Rh3CG189800 Rh3CG197100 Rh3DG175800
rosa_wichuraiana	Rw1G035410 Rw3G016750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	466
AccB1I	GGYRCC	1 cut(s)	380
AccBSI	CCGCTC	1 cut(s)	1237
AciI	CCGC	6 cut(s)	162, 353, 1114, 1149, 1237, 1287
AclWI	GGATC	8 cut(s)	172, 391, 517, 832, 899, 1274, 1278, 1291
AcoI	YGGCCR	2 cut(s)	3, 702
AcsI	RAATTY	4 cut(s)	211, 515, 764, 1310
AcuI	CTGAAG	2 cut(s)	611, 872
AfaI	GTAC	4 cut(s)	282, 456, 614, 1334
AfiI	CCNNNNNNNGG	1 cut(s)	535
AgsI	TTSAA	8 cut(s)	149, 550, 689, 799, 805, 902, 1057, 1094
AjnI	CCWGG	1 cut(s)	915
AjuI	GAANNNNNNNTTGG	2 cut(s)	106, 138
Alw21I	GWGCWC	1 cut(s)	1222
Alw26I	GTCTC	2 cut(s)	363, 837
AlwI	GGATC	8 cut(s)	172, 391, 517, 832, 899, 1274, 1278, 1291
AoxI	GGCC	2 cut(s)	3, 702
ApeKI	GCWGC	4 cut(s)	104, 448, 755, 1289
ApoI	RAATTY	4 cut(s)	211, 515, 764, 1310
AspS9I	GGNCC	3 cut(s)	224, 244, 1318
AsuHPI	GGTGA	5 cut(s)	578, 971, 1072, 1133, 1163
AvaII	GGWCC	3 cut(s)	224, 244, 1318
BaeGI	GKGCMC	1 cut(s)	385
BalI	TGGCCA	1 cut(s)	5
BamHI	GGATCC	1 cut(s)	1283
BanI	GGYRCC	1 cut(s)	380
BanII	GRGCYC	1 cut(s)	1222
BarI	GAAGNNNNNNTAC	2 cut(s)	845, 877
Bbv12I	GWGCWC	1 cut(s)	1222
BbvI	GCAGC	4 cut(s)	91, 435, 742, 1301
BccI	CCATC	5 cut(s)	80, 152, 287, 559, 815
BceAI	ACGGC	2 cut(s)	30, 717
BciT130I	CCWGG	1 cut(s)	917
BciVI	GTATCC	2 cut(s)	894, 1156
BcoDI	GTCTC	2 cut(s)	363, 837
BfaI	CTAG	2 cut(s)	362, 503
BfuAI	ACCTGC	1 cut(s)	466
BfuI	GTATCC	2 cut(s)	894, 1156
BisI	GCNGC	5 cut(s)	105, 449, 756, 1238, 1290
BlsI	GCNGC	5 cut(s)	106, 450, 757, 1239, 1291
Bme1390I	CCNGG	1 cut(s)	917
Bme18I	GGWCC	3 cut(s)	224, 244, 1318
BmgT120I	GGNCC	3 cut(s)	224, 244, 1318
BmiI	GGNNCC	7 cut(s)	91, 246, 382, 1285, 1319, 1320, 1329
BmrFI	CCNGG	1 cut(s)	917
BmrI	ACTGGG	1 cut(s)	1316
BmsI	GCATC	2 cut(s)	548, 613
BmuI	ACTGGG	1 cut(s)	1316
BpmI	CTGGAG	1 cut(s)	861
BpuEI	CTTGAG	2 cut(s)	176, 696
BsaJI	CCNNGG	2 cut(s)	46, 441
BsaWI	WCCGGW	1 cut(s)	1214
BsaXI	ACNNNNNCTCC	2 cut(s)	1204, 1234
Bsc4I	CCNNNNNNNGG	1 cut(s)	535
Bse118I	RCCGGY	2 cut(s)	377, 1301
Bse1I	ACTGG	2 cut(s)	730, 1322
Bse3DI	GCAATG	3 cut(s)	513, 1048, 1170
BseBI	CCWGG	1 cut(s)	917
BseDI	CCNNGG	2 cut(s)	46, 441
BseGI	GGATG	4 cut(s)	91, 646, 754, 925
BseLI	CCNNNNNNNGG	1 cut(s)	535
BseMI	GCAATG	3 cut(s)	513, 1048, 1170
BseMII	CTCAG	4 cut(s)	143, 774, 870, 1149
BseNI	ACTGG	2 cut(s)	730, 1322
BseRI	GAGGAG	2 cut(s)	1286, 1289
BseSI	GKGCMC	1 cut(s)	385
BseXI	GCAGC	4 cut(s)	91, 435, 742, 1301
BseYI	CCCAGC	1 cut(s)	1122
BsgI	GTGCAG	1 cut(s)	1246
BshFI	GGCC	2 cut(s)	5, 704
BshNI	GGYRCC	1 cut(s)	380
BsiHKAI	GWGCWC	1 cut(s)	1222
BsiSI	CCGG	3 cut(s)	378, 1215, 1302
BslFI	GGGAC	2 cut(s)	1215, 1304
BslI	CCNNNNNNNGG	1 cut(s)	535
BsmAI	GTCTC	2 cut(s)	363, 837
BsmFI	GGGAC	2 cut(s)	1215, 1304
BsnI	GGCC	2 cut(s)	5, 704
Bsp1286I	GDGCHC	2 cut(s)	385, 1222
BspACI	CCGC	6 cut(s)	162, 353, 1114, 1149, 1237, 1287
BspANI	GGCC	2 cut(s)	5, 704
BspCNI	CTCAG	4 cut(s)	144, 773, 871, 1150
BspHI	TCATGA	1 cut(s)	519
BspLI	GGNNCC	7 cut(s)	91, 246, 382, 1285, 1319, 1320, 1329
BspMI	ACCTGC	1 cut(s)	466
BspPI	GGATC	8 cut(s)	172, 391, 517, 832, 899, 1274, 1278, 1291
BspT107I	GGYRCC	1 cut(s)	380
BsrBI	CCGCTC	1 cut(s)	1237
BsrDI	GCAATG	3 cut(s)	513, 1048, 1170
BsrFI	RCCGGY	2 cut(s)	377, 1301
BsrI	ACTGG	2 cut(s)	730, 1322
BssAI	RCCGGY	2 cut(s)	377, 1301
BssECI	CCNNGG	2 cut(s)	46, 441
BssT1I	CCWWGG	1 cut(s)	441
Bst2UI	CCWGG	1 cut(s)	917
Bst4CI	ACNGT	5 cut(s)	61, 81, 301, 848, 1332
Bst6I	CTCTTC	1 cut(s)	804
BstAPI	GCANNNNNTGC	2 cut(s)	104, 1298
BstC8I	GCNNGC	4 cut(s)	493, 1116, 1120, 1242
BstDEI	CTNAG	5 cut(s)	152, 760, 879, 1158, 1262
BstDSI	CCRYGG	1 cut(s)	46
BstEII	GGTNACC	1 cut(s)	1078
BstF5I	GGATG	4 cut(s)	91, 646, 754, 925
BstMAI	GTCTC	2 cut(s)	363, 837
BstMWI	GCNNNNNNNGC	5 cut(s)	104, 350, 425, 1181, 1298
BstNI	CCWGG	1 cut(s)	917
BstPI	GGTNACC	1 cut(s)	1078
BstSCI	CCNGG	1 cut(s)	915
BstSLI	GKGCMC	1 cut(s)	385
BstV1I	GCAGC	4 cut(s)	91, 435, 742, 1301
BstX2I	RGATCY	3 cut(s)	396, 904, 1283
BstYI	RGATCY	3 cut(s)	396, 904, 1283
BsuI	GTATCC	2 cut(s)	894, 1156
BsuRI	GGCC	2 cut(s)	5, 704
BtgI	CCRYGG	1 cut(s)	46
BtgZI	GCGATG	2 cut(s)	572, 996
BtsCI	GGATG	4 cut(s)	91, 646, 754, 925
BtsIMutI	CAGTG	2 cut(s)	77, 1329
BveI	ACCTGC	1 cut(s)	466
Cac8I	GCNNGC	4 cut(s)	493, 1116, 1120, 1242
CciI	TCATGA	1 cut(s)	519
Cfr10I	RCCGGY	2 cut(s)	377, 1301
Cfr13I	GGNCC	3 cut(s)	224, 244, 1318
Csp6I	GTAC	4 cut(s)	281, 455, 613, 1333
CspCI	CAANNNNNGTGG	2 cut(s)	616, 651
CviAII	CATG	3 cut(s)	488, 520, 1132
CviQI	GTAC	4 cut(s)	281, 455, 613, 1333
DdeI	CTNAG	5 cut(s)	152, 760, 879, 1158, 1262
EaeI	YGGCCR	2 cut(s)	3, 702
Eam1104I	CTCTTC	1 cut(s)	804
EarI	CTCTTC	1 cut(s)	804
EciI	GGCGGA	1 cut(s)	177
Ecl136II	GAGCTC	1 cut(s)	1220
Eco130I	CCWWGG	1 cut(s)	441
Eco24I	GRGCYC	1 cut(s)	1222
Eco32I	GATATC	1 cut(s)	837
Eco47I	GGWCC	3 cut(s)	224, 244, 1318
Eco53kI	GAGCTC	1 cut(s)	1220
Eco57I	CTGAAG	2 cut(s)	611, 872
Eco91I	GGTNACC	1 cut(s)	1078
EcoICRI	GAGCTC	1 cut(s)	1220
EcoO109I	RGGNCCY	2 cut(s)	244, 1318
EcoO65I	GGTNACC	1 cut(s)	1078
EcoRI	GAATTC	3 cut(s)	211, 515, 764
EcoRII	CCWGG	1 cut(s)	915
EcoRV	GATATC	1 cut(s)	837
EcoT14I	CCWWGG	1 cut(s)	441
EcoT38I	GRGCYC	1 cut(s)	1222
ErhI	CCWWGG	1 cut(s)	441
FaeI	CATG	3 cut(s)	491, 523, 1135
FalI	AAGNNNNNCTT	4 cut(s)	106, 138, 669, 701
FaqI	GGGAC	2 cut(s)	1215, 1304
FatI	CATG	3 cut(s)	487, 519, 1131
FauI	CCCGC	1 cut(s)	1107
Fnu4HI	GCNGC	5 cut(s)	105, 449, 756, 1238, 1290
FokI	GGATG	4 cut(s)	98, 653, 761, 932
FriOI	GRGCYC	1 cut(s)	1222
Fsp4HI	GCNGC	5 cut(s)	105, 449, 756, 1238, 1290
FspBI	CTAG	2 cut(s)	362, 503
GluI	GCNGC	5 cut(s)	105, 449, 756, 1238, 1290
GsaI	CCCAGC	1 cut(s)	1126
GsuI	CTGGAG	1 cut(s)	861
HaeIII	GGCC	2 cut(s)	5, 704
HapII	CCGG	3 cut(s)	378, 1215, 1302
Hin1II	CATG	3 cut(s)	491, 523, 1135
HincII	GTYRAC	2 cut(s)	304, 1197
HindII	GTYRAC	2 cut(s)	304, 1197
HindIII	AAGCTT	1 cut(s)	14
HinfI	GANTC	2 cut(s)	52, 1063
HpaI	GTTAAC	1 cut(s)	304
HpaII	CCGG	3 cut(s)	378, 1215, 1302
HphI	GGTGA	5 cut(s)	578, 971, 1072, 1133, 1163
Hpy166II	GTNNAC	4 cut(s)	304, 391, 862, 1197
Hpy188I	TCNGA	5 cut(s)	85, 335, 763, 852, 1234
Hpy188III	TCNNGA	4 cut(s)	520, 668, 686, 840
Hpy8I	GTNNAC	4 cut(s)	304, 391, 862, 1197
Hpy99I	CGWCG	1 cut(s)	65
HpyAV	CCTTC	4 cut(s)	586, 638, 1051, 1058
HpyCH4III	ACNGT	5 cut(s)	61, 81, 301, 848, 1332
HpyCH4V	TGCA	9 cut(s)	321, 372, 491, 511, 656, 938, 1041, 1227, 1307
HpyF10VI	GCNNNNNNNGC	5 cut(s)	104, 350, 425, 1181, 1298
HpyF3I	CTNAG	5 cut(s)	152, 760, 879, 1158, 1262
Hsp92II	CATG	3 cut(s)	491, 523, 1135
KflI	GGGWCCC	1 cut(s)	1318
KspAI	GTTAAC	1 cut(s)	304
LmnI	GCTCC	4 cut(s)	335, 1217, 1225, 1276
Lsp1109I	GCAGC	4 cut(s)	91, 435, 742, 1301
LweI	GCATC	2 cut(s)	548, 613
MaeI	CTAG	2 cut(s)	362, 503
MaeIII	GTNAC	4 cut(s)	73, 994, 1078, 1139
MbiI	CCGCTC	1 cut(s)	1237
MboII	GAAGA	7 cut(s)	28, 574, 619, 821, 865, 914, 980
MfeI	CAATTG	2 cut(s)	888, 897
MflI	RGATCY	3 cut(s)	396, 904, 1283
MhlI	GDGCHC	2 cut(s)	385, 1222
MlsI	TGGCCA	1 cut(s)	5
MluNI	TGGCCA	1 cut(s)	5
MmeI	TCCRAC	1 cut(s)	63
MnlI	CCTC	9 cut(s)	200, 411, 473, 536, 673, 769, 1153, 1264, 1267
Mox20I	TGGCCA	1 cut(s)	5
MscI	TGGCCA	1 cut(s)	5
MseI	TTAA	6 cut(s)	21, 303, 465, 474, 603, 1209
MslI	CAYNNNNRTG	1 cut(s)	743
Msp20I	TGGCCA	1 cut(s)	5
MspI	CCGG	3 cut(s)	378, 1215, 1302
MspR9I	CCNGG	1 cut(s)	917
MunI	CAATTG	2 cut(s)	888, 897
MvaI	CCWGG	1 cut(s)	917
MwoI	GCNNNNNNNGC	5 cut(s)	104, 350, 425, 1181, 1298
NlaIII	CATG	3 cut(s)	491, 523, 1135
NlaIV	GGNNCC	7 cut(s)	91, 246, 382, 1285, 1319, 1320, 1329
NmuCI	GTSAC	2 cut(s)	994, 1139
PagI	TCATGA	1 cut(s)	519
PfeI	GAWTC	2 cut(s)	52, 1063
PkrI	GCNGC	5 cut(s)	106, 450, 757, 1239, 1291
PpuMI	RGGWCCY	2 cut(s)	244, 1318
Psp124BI	GAGCTC	1 cut(s)	1222
Psp5II	RGGWCCY	2 cut(s)	244, 1318
Psp6I	CCWGG	1 cut(s)	915
PspEI	GGTNACC	1 cut(s)	1078
PspFI	CCCAGC	1 cut(s)	1122
PspGI	CCWGG	1 cut(s)	915
PspN4I	GGNNCC	7 cut(s)	91, 246, 382, 1285, 1319, 1320, 1329
PspPI	GGNCC	3 cut(s)	224, 244, 1318
PspPPI	RGGWCCY	2 cut(s)	244, 1318
PsuI	RGATCY	3 cut(s)	396, 904, 1283
RsaI	GTAC	4 cut(s)	282, 456, 614, 1334
RsaNI	GTAC	4 cut(s)	281, 455, 613, 1333
RseI	CAYNNNNRTG	1 cut(s)	743
SacI	GAGCTC	1 cut(s)	1222
SaqAI	TTAA	6 cut(s)	21, 303, 465, 474, 603, 1209
SatI	GCNGC	5 cut(s)	105, 449, 756, 1238, 1290
Sau96I	GGNCC	3 cut(s)	224, 244, 1318
ScrFI	CCNGG	1 cut(s)	917
SduI	GDGCHC	2 cut(s)	385, 1222
SfaNI	GCATC	2 cut(s)	548, 613
SinI	GGWCC	3 cut(s)	224, 244, 1318
SmiMI	CAYNNNNRTG	1 cut(s)	743
SmlI	CTYRAG	2 cut(s)	191, 711
SmoI	CTYRAG	2 cut(s)	191, 711
SsiI	CCGC	6 cut(s)	162, 353, 1114, 1149, 1237, 1287
SspI	AATATT	1 cut(s)	664
SspMI	CTAG	2 cut(s)	362, 503
SstI	GAGCTC	1 cut(s)	1222
StyD4I	CCNGG	1 cut(s)	915
StyI	CCWWGG	1 cut(s)	441
TaaI	ACNGT	5 cut(s)	61, 81, 301, 848, 1332
TaqI	TCGA	7 cut(s)	39, 63, 167, 215, 267, 291, 1269
TauI	GCSGC	1 cut(s)	1240
TfiI	GAWTC	2 cut(s)	52, 1063
Tru1I	TTAA	6 cut(s)	21, 303, 465, 474, 603, 1209
Tru9I	TTAA	6 cut(s)	21, 303, 465, 474, 603, 1209
TscAI	CASTG	2 cut(s)	84, 1329
TseFI	GTSAC	2 cut(s)	994, 1139
TseI	GCWGC	4 cut(s)	104, 448, 755, 1289
Tsp45I	GTSAC	2 cut(s)	994, 1139
TspDTI	ATGAA	5 cut(s)	153, 508, 575, 657, 1159
TspGWI	ACGGA	4 cut(s)	492, 630, 897, 1212
TspRI	CASTG	2 cut(s)	84, 1329
VpaK11BI	GGWCC	3 cut(s)	224, 244, 1318
XapI	RAATTY	4 cut(s)	211, 515, 764, 1310
XspI	CTAG	2 cut(s)	362, 503

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.