Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr1B

Orthogroup ID

OG0002577

Physical Location & Seq

Genomic Coordinates Forward (+)

49481909 .. 49484920

Gene Structure 3012 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh1BG365700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1212 bp

ATGGCGACCACGATCAAGCTTATCAAGGCCCGTCAGATCTTCGACAGCCGTGGAAATCCCACCGTTGAAGCTGTGGAGAATGTGAATGGCATCATTGGACCTGCCTTGATTGGAAAGGACCCAACAGAACAGACCCAAATTGACAATTATATGGTTCAACAGCTTGATGGAACTACAAATGAATGGGGTTGGTGCAAACAGAAGCTTGGAGCAAATGCAATATTAGCAGTGTCTCTTGCTGTTTGTAAAGCAGGTGCCATTGTAAAAAAGATACCCCTATACAAGCACATAGCCAATCTTGCTGGAAACAAGACCTTGGTTCTGCCTGTTCCTGCATTTAATGTCATCAATGGAGGTTCTCATGCTGGCAATAAACTAGCCATGCAGGAATTTATGATTCTCCCCGTTGGAGCATCTTCTTTTAAGGAAGCCATGAAGATGGGTGTAGAAGTATATCATCATCTGAAGGCTGTAATTAAGAAGAAGTATGGACAAGATGCCACAAATGTTGGGGATGAAGGTGGCTTTGCACCCAATATTCAGGAAAACAAAGAGGGTCTTGAACTTCTGAAGACAGCTATTGCTAAAGCTGGATATACCGGAAAGGTTGTTATCGGGATGGATGTTGCTGCTTCAGAATTCTATGATGATAAGGATAAGACTTATGATTTGAATTTCAAGGAAGAGAAAAATGACGGATCACAAAGAATATCAGGAGACAGTCTGAAGAATGTTTACAAGTCATTTGTTACAGAGTATCCAATTGTGTCAATTGAAGATCCCTTTGATCAGGATGACTGGGAACACTATGCTAAGATGACTGGTGAAATTGGCGAGCAAGTGCAAATTGTTGGTGATGATCTCCTTGTTACAAACCCAAAGCGAGTGGAGAAAGCAATCAAGGAGAAGAGCTGCAATGCCCTTCTTTTGAAGGTGAATCAAATTGGTTCAGTAACCGAAAGTATTGAAGCTGTGAAAATGTCAAAACGTGCTGGATGGGGTGTCATGGCAAGTCACCGAAGTGGTGAAACTGAGGATACTTTCATTGCTGACCTTTCTGTGGGGCTTGCAACGGGCCAGATTAAGACTGGAGCTCCATGCAGATCCGAGCGTCTTGCTAAGTACAACCAGCTTCTTAGAATTGAAGAGGAGCTTGGGTCTGCAGCAGTCTATGCTGGATCAAAATTCAGAGTACCAGTGGAGCCATACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00010 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko03018 ko04066 map00010 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map03018 map04066

Pfam Domains

Protein Families

Enolase_C Enolase_N

Protein Analysis

403

Amino Acids

43.91

Weight (kDa)

6.16

Isoelectric Point (pI)

38.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Enolase_N	PF03952	23 - 97	5.4e-21	Enolase, N-terminal domain
Enolase_C	PF00113	106 - 397	3.4e-159	Enolase, C-terminal TIM barrel domain
MR_MLE_C	PF13378	232 - 364	2.7e-08	Enolase C-terminal domain-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002577)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G36530 AT2G36530
fragaria_vesca	FvH4_6g17770 FvH4_6g17770 FvH4_7g28150
malus_domestica	MD01G1185500.v1.1 MD12G1016600.v1.1 MD14G1014600.v1.1
prunus_persica	Prupe.2G281900_v2.0.a1 Prupe.2G281900_v2.0.a1 Prupe.7G118700_v2.0.a1
pyrus_communis	pycom01g19710 pycom12g01360 pycom14g01220
rosa_chinensis	RchiOBHm_Chr1g0375091 RchiOBHm_Chr3g0472001
rosa_laevigata	RLG00000000873 RLG00000024113
rosa_multiflora	Rmu_sc0000090.1_g000028 Rmu_sc0000554.1_g000017 Rmu_sc0002532.1_g000001 Rmu_sc0034562.1_g000001 Rmu_sc0039689.1_g000001
rosa_roxburghii	Rroxscaffold_4G00283020 Rroxscaffold_6G00409370
rosa_rugosa	Rorug01G0388300 Rorug01G0388400 Rorug03G0121500 Rorug03G0121600
rosa_samantha	Rh1AG398800 Rh1BG365700 Rh1CG378600 Rh1DG396400 Rh3AG173200 Rh3BG198600 Rh3CG189800 Rh3CG197100 Rh3DG175800
rosa_wichuraiana	Rw1G035410 Rw3G016750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	242
Acc36I	ACCTGC	2 cut(s)	109, 242
AccB1I	GGYRCC	1 cut(s)	254
AclWI	GGATC	4 cut(s)	706, 773, 1098, 1186
AcsI	RAATTY	4 cut(s)	389, 638, 673, 1184
AcuI	CTGAAG	4 cut(s)	485, 590, 618, 746
AfaI	GTAC	2 cut(s)	1124, 1194
AfiI	CCNNNNNNNGG	1 cut(s)	1060
AgsI	TTSAA	9 cut(s)	68, 158, 563, 673, 679, 776, 931, 968, 1145
AjuI	GAANNNNNNNTTGG	2 cut(s)	1137, 1169
AleI	CACNNNNGTG	1 cut(s)	1020
Alw21I	GWGCWC	1 cut(s)	1096
Alw26I	GTCTC	2 cut(s)	237, 711
AlwI	GGATC	4 cut(s)	706, 773, 1098, 1186
AoxI	GGCC	2 cut(s)	27, 1075
ApeKI	GCWGC	3 cut(s)	629, 912, 1163
ApoI	RAATTY	4 cut(s)	389, 638, 673, 1184
ArsI	GACNNNNNNTTYG	2 cut(s)	652, 684
AspS9I	GGNCC	4 cut(s)	28, 98, 118, 1075
AsuHPI	GGTGA	5 cut(s)	836, 866, 946, 1007, 1037
AvaII	GGWCC	2 cut(s)	98, 118
BanI	GGYRCC	1 cut(s)	254
BanII	GRGCYC	1 cut(s)	1096
BarI	GAAGNNNNNNTAC	2 cut(s)	719, 751
BbsI	GAAGAC	1 cut(s)	578
Bbv12I	GWGCWC	1 cut(s)	1096
BbvI	GCAGC	3 cut(s)	616, 899, 1175
BccI	CCATC	4 cut(s)	161, 433, 613, 990
BceAI	ACGGC	1 cut(s)	33
BcgI	CGANNNNNNTGC	2 cut(s)	1097, 1131
BciVI	GTATCC	2 cut(s)	768, 1030
BclI	TGATCA	1 cut(s)	787
BcoDI	GTCTC	2 cut(s)	237, 711
BfaI	CTAG	1 cut(s)	377
BfmI	CTRYAG	1 cut(s)	1161
BfuAI	ACCTGC	2 cut(s)	109, 242
BfuI	GTATCC	2 cut(s)	768, 1030
BglII	AGATCT	1 cut(s)	36
BisI	GCNGC	3 cut(s)	630, 913, 1164
BlsI	GCNGC	3 cut(s)	631, 914, 1165
Bme18I	GGWCC	2 cut(s)	98, 118
BmgT120I	GGNCC	4 cut(s)	28, 98, 118, 1075
BmiI	GGNNCC	3 cut(s)	120, 256, 1203
BmrI	ACTGGG	1 cut(s)	808
BmsI	GCATC	3 cut(s)	99, 422, 487
BmuI	ACTGGG	1 cut(s)	808
BpiI	GAAGAC	1 cut(s)	578
BpmI	CTGGAG	1 cut(s)	1110
BsaJI	CCNNGG	2 cut(s)	49, 315
BsaWI	WCCGGW	1 cut(s)	599
BsaXI	ACNNNNNCTCC	4 cut(s)	1078, 1108, 1142, 1172
Bsc4I	CCNNNNNNNGG	1 cut(s)	1060
Bse1I	ACTGG	4 cut(s)	803, 826, 1093, 1196
Bse3DI	GCAATG	2 cut(s)	922, 1044
BseDI	CCNNGG	2 cut(s)	49, 315
BseGI	GGATG	5 cut(s)	520, 624, 628, 799, 1001
BseLI	CCNNNNNNNGG	1 cut(s)	1060
BseMI	GCAATG	2 cut(s)	922, 1044
BseMII	CTCAG	1 cut(s)	1023
BseNI	ACTGG	4 cut(s)	803, 826, 1093, 1196
BseRI	GAGGAG	1 cut(s)	1163
BseXI	GCAGC	3 cut(s)	616, 899, 1175
BshFI	GGCC	2 cut(s)	29, 1077
BshNI	GGYRCC	1 cut(s)	254
BsiHKAI	GWGCWC	1 cut(s)	1096
BsiSI	CCGG	1 cut(s)	600
BslI	CCNNNNNNNGG	1 cut(s)	1060
BsmAI	GTCTC	2 cut(s)	237, 711
BsnI	GGCC	2 cut(s)	29, 1077
Bsp1286I	GDGCHC	1 cut(s)	1096
Bsp143I	GATC	8 cut(s)	12, 36, 698, 778, 787, 859, 1103, 1178
BspANI	GGCC	2 cut(s)	29, 1077
BspCNI	CTCAG	1 cut(s)	1024
BspLI	GGNNCC	3 cut(s)	120, 256, 1203
BspMAI	CTGCAG	1 cut(s)	1165
BspMI	ACCTGC	2 cut(s)	109, 242
BspPI	GGATC	4 cut(s)	706, 773, 1098, 1186
BspQI	GCTCTTC	1 cut(s)	902
BspT107I	GGYRCC	1 cut(s)	254
BsrDI	GCAATG	2 cut(s)	922, 1044
BsrI	ACTGG	4 cut(s)	803, 826, 1093, 1196
BssECI	CCNNGG	2 cut(s)	49, 315
BssMI	GATC	8 cut(s)	12, 36, 698, 778, 787, 859, 1103, 1178
BssT1I	CCWWGG	1 cut(s)	315
Bst4CI	ACNGT	2 cut(s)	64, 722
Bst6I	CTCTTC	3 cut(s)	678, 902, 1140
BstAPI	GCANNNNNTGC	1 cut(s)	1172
BstC8I	GCNNGC	3 cut(s)	367, 836, 1068
BstDEI	CTNAG	4 cut(s)	813, 1032, 1119, 1136
BstDSI	CCRYGG	1 cut(s)	49
BstF5I	GGATG	5 cut(s)	520, 624, 628, 799, 1001
BstKTI	GATC	8 cut(s)	15, 39, 701, 781, 790, 862, 1106, 1181
BstMAI	GTCTC	2 cut(s)	237, 711
BstMBI	GATC	8 cut(s)	12, 36, 698, 778, 787, 859, 1103, 1178
BstMWI	GCNNNNNNNGC	3 cut(s)	224, 299, 1172
BstSFI	CTRYAG	1 cut(s)	1161
BstV1I	GCAGC	3 cut(s)	616, 899, 1175
BstV2I	GAAGAC	1 cut(s)	578
BstX2I	RGATCY	3 cut(s)	36, 778, 1103
BstXI	CCANNNNNNTGG	1 cut(s)	439
BstYI	RGATCY	3 cut(s)	36, 778, 1103
BsuI	GTATCC	2 cut(s)	768, 1030
BsuRI	GGCC	2 cut(s)	29, 1077
BtgI	CCRYGG	1 cut(s)	49
BtsCI	GGATG	5 cut(s)	520, 624, 628, 799, 1001
BtsI	GCAGTG	1 cut(s)	234
BtsIMutI	CAGTG	2 cut(s)	234, 1203
BveI	ACCTGC	2 cut(s)	109, 242
Cac8I	GCNNGC	3 cut(s)	367, 836, 1068
Cfr13I	GGNCC	4 cut(s)	28, 98, 118, 1075
CseI	GACGC	1 cut(s)	1100
Csp6I	GTAC	2 cut(s)	1123, 1193
CspCI	CAANNNNNGTGG	4 cut(s)	490, 525, 867, 902
CviAII	CATG	5 cut(s)	362, 382, 433, 1006, 1098
CviQI	GTAC	2 cut(s)	1123, 1193
DdeI	CTNAG	4 cut(s)	813, 1032, 1119, 1136
DpnI	GATC	8 cut(s)	14, 38, 700, 780, 789, 861, 1105, 1180
DpnII	GATC	8 cut(s)	12, 36, 698, 778, 787, 859, 1103, 1178
Eam1104I	CTCTTC	3 cut(s)	678, 902, 1140
EarI	CTCTTC	3 cut(s)	678, 902, 1140
Ecl136II	GAGCTC	1 cut(s)	1094
Eco130I	CCWWGG	1 cut(s)	315
Eco24I	GRGCYC	1 cut(s)	1096
Eco47I	GGWCC	2 cut(s)	98, 118
Eco53kI	GAGCTC	1 cut(s)	1094
Eco57I	CTGAAG	4 cut(s)	485, 590, 618, 746
EcoICRI	GAGCTC	1 cut(s)	1094
EcoO109I	RGGNCCY	1 cut(s)	118
EcoRI	GAATTC	1 cut(s)	638
EcoT14I	CCWWGG	1 cut(s)	315
EcoT38I	GRGCYC	1 cut(s)	1096
ErhI	CCWWGG	1 cut(s)	315
FaeI	CATG	5 cut(s)	365, 385, 436, 1009, 1101
FalI	AAGNNNNNCTT	4 cut(s)	543, 575, 1137, 1169
FatI	CATG	5 cut(s)	361, 381, 432, 1005, 1097
FbaI	TGATCA	1 cut(s)	787
Fnu4HI	GCNGC	3 cut(s)	630, 913, 1164
FokI	GGATG	5 cut(s)	527, 631, 635, 806, 1008
FriOI	GRGCYC	1 cut(s)	1096
Fsp4HI	GCNGC	3 cut(s)	630, 913, 1164
FspBI	CTAG	1 cut(s)	377
GluI	GCNGC	3 cut(s)	630, 913, 1164
GsuI	CTGGAG	1 cut(s)	1110
HaeIII	GGCC	2 cut(s)	29, 1077
HapII	CCGG	1 cut(s)	600
HgaI	GACGC	1 cut(s)	1100
Hin1II	CATG	5 cut(s)	365, 385, 436, 1009, 1101
HindIII	AAGCTT	2 cut(s)	17, 203
HinfI	GANTC	2 cut(s)	397, 937
HpaII	CCGG	1 cut(s)	600
HphI	GGTGA	5 cut(s)	836, 866, 946, 1007, 1037
Hpy166II	GTNNAC	1 cut(s)	736
Hpy188I	TCNGA	7 cut(s)	36, 465, 570, 637, 726, 1108, 1190
Hpy188III	TCNNGA	5 cut(s)	542, 560, 616, 714, 791
Hpy8I	GTNNAC	1 cut(s)	736
HpyAV	CCTTC	4 cut(s)	460, 512, 925, 932
HpyCH4III	ACNGT	2 cut(s)	64, 722
HpyCH4IV	ACGT	1 cut(s)	988
HpyF10VI	GCNNNNNNNGC	3 cut(s)	224, 299, 1172
HpyF3I	CTNAG	4 cut(s)	813, 1032, 1119, 1136
HpySE526I	ACGT	1 cut(s)	988
Hsp92II	CATG	5 cut(s)	365, 385, 436, 1009, 1101
Ksp22I	TGATCA	1 cut(s)	787
Kzo9I	GATC	8 cut(s)	12, 36, 698, 778, 787, 859, 1103, 1178
LguI	GCTCTTC	1 cut(s)	902
LmnI	GCTCC	6 cut(s)	209, 410, 1091, 1099, 1150, 1201
Lsp1109I	GCAGC	3 cut(s)	616, 899, 1175
LweI	GCATC	3 cut(s)	99, 422, 487
MaeI	CTAG	1 cut(s)	377
MaeII	ACGT	1 cut(s)	988
MaeIII	GTNAC	4 cut(s)	748, 868, 952, 1013
MalI	GATC	8 cut(s)	14, 38, 700, 780, 789, 861, 1105, 1180
MboI	GATC	8 cut(s)	12, 36, 698, 778, 787, 859, 1103, 1178
MfeI	CAATTG	2 cut(s)	762, 771
MflI	RGATCY	3 cut(s)	36, 778, 1103
MhlI	GDGCHC	1 cut(s)	1096
MmeI	TCCRAC	1 cut(s)	388
MnlI	CCTC	4 cut(s)	347, 547, 1027, 1141
MseI	TTAA	4 cut(s)	339, 423, 477, 1083
MslI	CAYNNNNRTG	2 cut(s)	437, 1020
MspI	CCGG	1 cut(s)	600
MunI	CAATTG	2 cut(s)	762, 771
MwoI	GCNNNNNNNGC	3 cut(s)	224, 299, 1172
NdeII	GATC	8 cut(s)	12, 36, 698, 778, 787, 859, 1103, 1178
NlaIII	CATG	5 cut(s)	365, 385, 436, 1009, 1101
NlaIV	GGNNCC	3 cut(s)	120, 256, 1203
NmuCI	GTSAC	1 cut(s)	1013
OliI	CACNNNNGTG	1 cut(s)	1020
PaqCI	CACCTGC	1 cut(s)	242
PciSI	GCTCTTC	1 cut(s)	902
PfeI	GAWTC	2 cut(s)	397, 937
PkrI	GCNGC	3 cut(s)	631, 914, 1165
PpuMI	RGGWCCY	1 cut(s)	118
Psp124BI	GAGCTC	1 cut(s)	1096
Psp5II	RGGWCCY	1 cut(s)	118
PspN4I	GGNNCC	3 cut(s)	120, 256, 1203
PspPI	GGNCC	4 cut(s)	28, 98, 118, 1075
PspPPI	RGGWCCY	1 cut(s)	118
PstI	CTGCAG	1 cut(s)	1165
PsuI	RGATCY	3 cut(s)	36, 778, 1103
RsaI	GTAC	2 cut(s)	1124, 1194
RsaNI	GTAC	2 cut(s)	1123, 1193
RseI	CAYNNNNRTG	2 cut(s)	437, 1020
SacI	GAGCTC	1 cut(s)	1096
SapI	GCTCTTC	1 cut(s)	902
SaqAI	TTAA	4 cut(s)	339, 423, 477, 1083
SatI	GCNGC	3 cut(s)	630, 913, 1164
Sau3AI	GATC	8 cut(s)	12, 36, 698, 778, 787, 859, 1103, 1178
Sau96I	GGNCC	4 cut(s)	28, 98, 118, 1075
SduI	GDGCHC	1 cut(s)	1096
SfaNI	GCATC	3 cut(s)	99, 422, 487
SfcI	CTRYAG	1 cut(s)	1161
SinI	GGWCC	2 cut(s)	98, 118
SmiMI	CAYNNNNRTG	2 cut(s)	437, 1020
SspI	AATATT	2 cut(s)	222, 538
SspMI	CTAG	1 cut(s)	377
SstI	GAGCTC	1 cut(s)	1096
StyI	CCWWGG	1 cut(s)	315
TaaI	ACNGT	2 cut(s)	64, 722
TaiI	ACGT	1 cut(s)	991
TaqI	TCGA	1 cut(s)	42
TatI	WGTACW	1 cut(s)	1122
TfiI	GAWTC	2 cut(s)	397, 937
Tru1I	TTAA	4 cut(s)	339, 423, 477, 1083
Tru9I	TTAA	4 cut(s)	339, 423, 477, 1083
TscAI	CASTG	2 cut(s)	234, 1203
TseFI	GTSAC	1 cut(s)	1013
TseI	GCWGC	3 cut(s)	629, 912, 1163
Tsp45I	GTSAC	1 cut(s)	1013
TspDTI	ATGAA	4 cut(s)	195, 449, 531, 1033
TspGWI	ACGGA	1 cut(s)	711
TspRI	CASTG	2 cut(s)	234, 1203
VpaK11BI	GGWCC	2 cut(s)	98, 118
XapI	RAATTY	4 cut(s)	389, 638, 673, 1184
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1085
XspI	CTAG	1 cut(s)	377

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.