Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr1A

Orthogroup ID

OG0002577

Physical Location & Seq

Genomic Coordinates Reverse (-)

62634132 .. 62637615

Gene Structure 3484 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh1AG398800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1254 bp

ATGGCGACCACGATCAAGCTCATCAAGGCCCGTCAGATCTTCGACAGCCGTGGAAATCCCACCGTTGAAGTTGATGTTACTCTTTCCGATGGAACTCTGGCCAGAGCGGCCGTGCCGAGTGGAGCTTCCACAGGTGTATATGAAGCACTGGAGTTGAGAGATGGCGGATCGGATTACCTTGGGAAGGGTGTCCTGAAGGCTGTGGAGAATGTGAATGGCATCATTGGACCTGCCTTGATTGGAAAGGTACTACTGTATTTTTTTTTGTTCTTACGCGATTATATTCCTGCTCCAACGAGGCGATTGGTTGTTGATATATCTATTTCTTTGTTTTGTGCATTGCAGGACCCAACAGAACAGACCCAAATTGACAATTATATGGTTCAACAGCTTGATGGAACTACAAATGAATGGGGTTGGTGCAAACAGAAGCTTGGAGCAAATGCAATATTGGCAGTGTCTCTTGCTGTTTGCAAAGCAGGTGCCATTGTGAAAAAGATACCCCTATACAAGCACATAGCCAATCTTGCTGGAAACAAGACCTTGGTTCTGCCTGTTCCTGCATTTAATGTCATCAATGGAGGTTCTCATGCTGGCAATAAACTAGCCATGCAGGAATTTATGATTCTCCCTGTTGGAGCATCTTCTTTTAAGGAAGCCATGAAGATGGGTGTAGAAGTATATCATCATCTGAAGGCTGTAATTAAGAAGAAGTATGGACAAGATGCCACAAATGTTGGGGATGAAGGTGGCTTTGCACCCAATATTCAGGAAAACAAAGAGGGTCTTGAACTTCTGAAGACAGCTATTGCTAAAGCTGGATATACCGGAAAGGTTGTTATTGGGATGGATGTTGCTGCTTCAGAATTCTATGGTGATAAGGATAAGACTTATGATTTGAATTTCAAGGAAGAGAAAAATGACGGATCACAAAGAATATCAGGAGACAGTCTGAAGAATGTTTACAAGTCATTTGTTACAGAGTATCCAATTGTGTCAATTGAAGATCCCTTTGATCAGGATGACTGGGAACACTATGCTAAGATGACTGGTGAAATTGGCGAGCAAGTGCAAATTGTTGGTGATGATCTCCTTGTTACAAACCCAAAGCGAGTGGAGAAAGCAATCAAGGAGAAGAGCTGCAATGCCCTTCTTTTGAAGGTGAATCAAATTGGTTCAGTAACCGAAAGTATTGAAGCTGTGAAAATGTCAAAACGTGCTGGATGGGGTGTCATGGCAAGTCACCGAAGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00010 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko03018 ko04066 map00010 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map03018 map04066

Pfam Domains

Protein Families

Enolase_C Enolase_N

Protein Analysis

417

Amino Acids

45.39

Weight (kDa)

7.01

Isoelectric Point (pI)

34.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Enolase_N	PF03952	5 - 82	1.3e-30	Enolase, N-terminal domain
Enolase_N	PF03952	115 - 173	1.2e-14	Enolase, N-terminal domain
Enolase_C	PF00113	182 - 416	2.9e-122	Enolase, C-terminal TIM barrel domain
MR_MLE_C	PF13378	308 - 412	4.8e-07	Enolase C-terminal domain-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002577)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G36530 AT2G36530
fragaria_vesca	FvH4_6g17770 FvH4_6g17770 FvH4_7g28150
malus_domestica	MD01G1185500.v1.1 MD12G1016600.v1.1 MD14G1014600.v1.1
prunus_persica	Prupe.2G281900_v2.0.a1 Prupe.2G281900_v2.0.a1 Prupe.7G118700_v2.0.a1
pyrus_communis	pycom01g19710 pycom12g01360 pycom14g01220
rosa_chinensis	RchiOBHm_Chr1g0375091 RchiOBHm_Chr3g0472001
rosa_laevigata	RLG00000000873 RLG00000024113
rosa_multiflora	Rmu_sc0000090.1_g000028 Rmu_sc0000554.1_g000017 Rmu_sc0002532.1_g000001 Rmu_sc0034562.1_g000001 Rmu_sc0039689.1_g000001
rosa_roxburghii	Rroxscaffold_4G00283020 Rroxscaffold_6G00409370
rosa_rugosa	Rorug01G0388300 Rorug01G0388400 Rorug03G0121500 Rorug03G0121600
rosa_samantha	Rh1AG398800 Rh1BG365700 Rh1CG378600 Rh1DG396400 Rh3AG173200 Rh3BG198600 Rh3CG189800 Rh3CG197100 Rh3DG175800
rosa_wichuraiana	Rw1G035410 Rw3G016750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	470
Acc36I	ACCTGC	2 cut(s)	238, 470
AccB1I	GGYRCC	1 cut(s)	482
AccBSI	CCGCTC	1 cut(s)	107
AccII	CGCG	1 cut(s)	276
AciI	CCGC	2 cut(s)	107, 165
AclWI	GGATC	3 cut(s)	175, 934, 1001
AcoI	YGGCCR	2 cut(s)	99, 108
AcsI	RAATTY	3 cut(s)	617, 866, 901
AcuI	CTGAAG	5 cut(s)	215, 713, 818, 846, 974
AfaI	GTAC	1 cut(s)	249
AfiI	CCNNNNNNNGG	1 cut(s)	184
AgsI	TTSAA	8 cut(s)	68, 386, 791, 901, 907, 1004, 1159, 1196
AluBI	AGCT	8 cut(s)	19, 125, 391, 433, 806, 818, 1140, 1199
AluI	AGCT	8 cut(s)	19, 125, 391, 433, 806, 818, 1140, 1199
Alw26I	GTCTC	2 cut(s)	465, 939
AlwI	GGATC	3 cut(s)	175, 934, 1001
AoxI	GGCC	3 cut(s)	27, 99, 108
ApeKI	GCWGC	2 cut(s)	857, 1140
ApoI	RAATTY	3 cut(s)	617, 866, 901
ArsI	GACNNNNNNTTYG	2 cut(s)	880, 912
AspS9I	GGNCC	3 cut(s)	28, 227, 346
AsuHPI	GGTGA	5 cut(s)	887, 1064, 1094, 1174, 1235
AvaII	GGWCC	2 cut(s)	227, 346
BaeI	ACNNNNGTAYC	2 cut(s)	239, 272
BalI	TGGCCA	1 cut(s)	101
BanI	GGYRCC	1 cut(s)	482
BarI	GAAGNNNNNNTAC	2 cut(s)	947, 979
BbsI	GAAGAC	1 cut(s)	806
BbvI	GCAGC	2 cut(s)	844, 1127
BccI	CCATC	6 cut(s)	83, 155, 389, 661, 841, 1218
BceAI	ACGGC	2 cut(s)	33, 95
BciVI	GTATCC	1 cut(s)	996
BclI	TGATCA	1 cut(s)	1015
BcoDI	GTCTC	2 cut(s)	465, 939
BfaI	CTAG	1 cut(s)	605
BfuAI	ACCTGC	2 cut(s)	238, 470
BfuI	GTATCC	1 cut(s)	996
BglI	GCCNNNNNGGC	1 cut(s)	107
BglII	AGATCT	1 cut(s)	36
BisI	GCNGC	3 cut(s)	108, 858, 1141
BlsI	GCNGC	3 cut(s)	109, 859, 1142
Bme18I	GGWCC	2 cut(s)	227, 346
BmgT120I	GGNCC	3 cut(s)	28, 227, 346
BmiI	GGNNCC	2 cut(s)	348, 484
BmrI	ACTGGG	1 cut(s)	1036
BmsI	GCATC	3 cut(s)	228, 650, 715
BmuI	ACTGGG	1 cut(s)	1036
BpiI	GAAGAC	1 cut(s)	806
BpmI	CTGGAG	1 cut(s)	170
BsaJI	CCNNGG	3 cut(s)	49, 178, 543
BsaWI	WCCGGW	1 cut(s)	827
Bsc4I	CCNNNNNNNGG	1 cut(s)	184
Bse1I	ACTGG	3 cut(s)	153, 1031, 1054
Bse3DI	GCAATG	2 cut(s)	338, 1150
BseDI	CCNNGG	3 cut(s)	49, 178, 543
BseGI	GGATG	5 cut(s)	748, 852, 856, 1027, 1229
BseLI	CCNNNNNNNGG	1 cut(s)	184
BseMI	GCAATG	2 cut(s)	338, 1150
BseNI	ACTGG	3 cut(s)	153, 1031, 1054
BseX3I	CGGCCG	1 cut(s)	108
BseXI	GCAGC	2 cut(s)	844, 1127
Bsh1236I	CGCG	1 cut(s)	276
Bsh1285I	CGRYCG	1 cut(s)	111
BshFI	GGCC	3 cut(s)	29, 101, 110
BshNI	GGYRCC	1 cut(s)	482
BsiEI	CGRYCG	1 cut(s)	111
BsiSI	CCGG	1 cut(s)	828
BslI	CCNNNNNNNGG	1 cut(s)	184
BsmAI	GTCTC	2 cut(s)	465, 939
BsnI	GGCC	3 cut(s)	29, 101, 110
Bsp143I	GATC	7 cut(s)	12, 36, 167, 926, 1006, 1015, 1087
BspACI	CCGC	2 cut(s)	107, 165
BspANI	GGCC	3 cut(s)	29, 101, 110
BspFNI	CGCG	1 cut(s)	276
BspLI	GGNNCC	2 cut(s)	348, 484
BspMI	ACCTGC	2 cut(s)	238, 470
BspPI	GGATC	3 cut(s)	175, 934, 1001
BspQI	GCTCTTC	1 cut(s)	1130
BspT107I	GGYRCC	1 cut(s)	482
BsrBI	CCGCTC	1 cut(s)	107
BsrDI	GCAATG	2 cut(s)	338, 1150
BsrI	ACTGG	3 cut(s)	153, 1031, 1054
BssECI	CCNNGG	3 cut(s)	49, 178, 543
BssMI	GATC	7 cut(s)	12, 36, 167, 926, 1006, 1015, 1087
BssT1I	CCWWGG	2 cut(s)	178, 543
Bst4CI	ACNGT	3 cut(s)	64, 255, 950
Bst6I	CTCTTC	2 cut(s)	906, 1130
BstC8I	GCNNGC	2 cut(s)	595, 1064
BstDEI	CTNAG	1 cut(s)	1041
BstDSI	CCRYGG	1 cut(s)	49
BstENI	CCTNNNNNAGG	1 cut(s)	182
BstF5I	GGATG	5 cut(s)	748, 852, 856, 1027, 1229
BstFNI	CGCG	1 cut(s)	276
BstKTI	GATC	7 cut(s)	15, 39, 170, 929, 1009, 1018, 1090
BstMAI	GTCTC	2 cut(s)	465, 939
BstMBI	GATC	7 cut(s)	12, 36, 167, 926, 1006, 1015, 1087
BstMCI	CGRYCG	1 cut(s)	111
BstMWI	GCNNNNNNNGC	3 cut(s)	107, 452, 527
BstUI	CGCG	1 cut(s)	276
BstV1I	GCAGC	2 cut(s)	844, 1127
BstV2I	GAAGAC	1 cut(s)	806
BstX2I	RGATCY	2 cut(s)	36, 1006
BstXI	CCANNNNNNTGG	1 cut(s)	667
BstYI	RGATCY	2 cut(s)	36, 1006
BstZI	CGGCCG	1 cut(s)	108
BsuI	GTATCC	1 cut(s)	996
BsuRI	GGCC	3 cut(s)	29, 101, 110
BtgI	CCRYGG	1 cut(s)	49
BtsCI	GGATG	5 cut(s)	748, 852, 856, 1027, 1229
BtsI	GCAGTG	1 cut(s)	462
BtsIMutI	CAGTG	2 cut(s)	146, 462
BveI	ACCTGC	2 cut(s)	238, 470
Cac8I	GCNNGC	2 cut(s)	595, 1064
Cfr13I	GGNCC	3 cut(s)	28, 227, 346
Csp6I	GTAC	1 cut(s)	248
CspCI	CAANNNNNGTGG	4 cut(s)	718, 753, 1095, 1130
CviAII	CATG	4 cut(s)	590, 610, 661, 1234
CviQI	GTAC	1 cut(s)	248
DdeI	CTNAG	1 cut(s)	1041
DpnI	GATC	7 cut(s)	14, 38, 169, 928, 1008, 1017, 1089
DpnII	GATC	7 cut(s)	12, 36, 167, 926, 1006, 1015, 1087
EaeI	YGGCCR	2 cut(s)	99, 108
EagI	CGGCCG	1 cut(s)	108
Eam1104I	CTCTTC	2 cut(s)	906, 1130
EarI	CTCTTC	2 cut(s)	906, 1130
EciI	GGCGGA	1 cut(s)	180
EclXI	CGGCCG	1 cut(s)	108
Eco130I	CCWWGG	2 cut(s)	178, 543
Eco47I	GGWCC	2 cut(s)	227, 346
Eco52I	CGGCCG	1 cut(s)	108
Eco57I	CTGAAG	5 cut(s)	215, 713, 818, 846, 974
EcoNI	CCTNNNNNAGG	1 cut(s)	182
EcoO109I	RGGNCCY	1 cut(s)	346
EcoRI	GAATTC	1 cut(s)	866
EcoT14I	CCWWGG	2 cut(s)	178, 543
ErhI	CCWWGG	2 cut(s)	178, 543
FaeI	CATG	4 cut(s)	593, 613, 664, 1237
FalI	AAGNNNNNCTT	2 cut(s)	771, 803
FatI	CATG	4 cut(s)	589, 609, 660, 1233
FbaI	TGATCA	1 cut(s)	1015
Fnu4HI	GCNGC	3 cut(s)	108, 858, 1141
FokI	GGATG	5 cut(s)	755, 859, 863, 1034, 1236
Fsp4HI	GCNGC	3 cut(s)	108, 858, 1141
FspBI	CTAG	1 cut(s)	605
GluI	GCNGC	3 cut(s)	108, 858, 1141
GsuI	CTGGAG	1 cut(s)	170
HaeIII	GGCC	3 cut(s)	29, 101, 110
HapII	CCGG	1 cut(s)	828
Hin1II	CATG	4 cut(s)	593, 613, 664, 1237
HindIII	AAGCTT	1 cut(s)	431
HinfI	GANTC	2 cut(s)	625, 1165
HpaII	CCGG	1 cut(s)	828
HphI	GGTGA	5 cut(s)	887, 1064, 1094, 1174, 1235
Hpy166II	GTNNAC	1 cut(s)	964
Hpy188I	TCNGA	7 cut(s)	36, 88, 172, 693, 798, 865, 954
Hpy188III	TCNNGA	5 cut(s)	193, 770, 788, 942, 1019
Hpy8I	GTNNAC	1 cut(s)	964
HpyAV	CCTTC	7 cut(s)	178, 190, 688, 740, 1153, 1160, 1242
HpyCH4III	ACNGT	3 cut(s)	64, 255, 950
HpyCH4IV	ACGT	1 cut(s)	1216
HpyF10VI	GCNNNNNNNGC	3 cut(s)	107, 452, 527
HpyF3I	CTNAG	1 cut(s)	1041
HpySE526I	ACGT	1 cut(s)	1216
Hsp92II	CATG	4 cut(s)	593, 613, 664, 1237
Ksp22I	TGATCA	1 cut(s)	1015
Kzo9I	GATC	7 cut(s)	12, 36, 167, 926, 1006, 1015, 1087
LguI	GCTCTTC	1 cut(s)	1130
LmnI	GCTCC	4 cut(s)	122, 295, 437, 638
Lsp1109I	GCAGC	2 cut(s)	844, 1127
LweI	GCATC	3 cut(s)	228, 650, 715
MaeI	CTAG	1 cut(s)	605
MaeII	ACGT	1 cut(s)	1216
MaeIII	GTNAC	5 cut(s)	76, 976, 1096, 1180, 1241
MalI	GATC	7 cut(s)	14, 38, 169, 928, 1008, 1017, 1089
MbiI	CCGCTC	1 cut(s)	107
MboI	GATC	7 cut(s)	12, 36, 167, 926, 1006, 1015, 1087
MboII	GAAGA	9 cut(s)	31, 636, 676, 721, 811, 923, 967, 1016, 1147
MfeI	CAATTG	2 cut(s)	990, 999
MflI	RGATCY	2 cut(s)	36, 1006
MlsI	TGGCCA	1 cut(s)	101
MluNI	TGGCCA	1 cut(s)	101
MmeI	TCCRAC	2 cut(s)	317, 616
MnlI	CCTC	3 cut(s)	291, 575, 775
Mox20I	TGGCCA	1 cut(s)	101
MscI	TGGCCA	1 cut(s)	101
MseI	TTAA	3 cut(s)	567, 651, 705
MslI	CAYNNNNRTG	1 cut(s)	665
Msp20I	TGGCCA	1 cut(s)	101
MspI	CCGG	1 cut(s)	828
MunI	CAATTG	2 cut(s)	990, 999
MvnI	CGCG	1 cut(s)	276
MwoI	GCNNNNNNNGC	3 cut(s)	107, 452, 527
NdeII	GATC	7 cut(s)	12, 36, 167, 926, 1006, 1015, 1087
NlaIII	CATG	4 cut(s)	593, 613, 664, 1237
NlaIV	GGNNCC	2 cut(s)	348, 484
NmeAIII	GCCGAG	1 cut(s)	141
NmuCI	GTSAC	1 cut(s)	1241
PaqCI	CACCTGC	1 cut(s)	470
PciSI	GCTCTTC	1 cut(s)	1130
PfeI	GAWTC	2 cut(s)	625, 1165
PkrI	GCNGC	3 cut(s)	109, 859, 1142
PpuMI	RGGWCCY	1 cut(s)	346
Psp5II	RGGWCCY	1 cut(s)	346
PspN4I	GGNNCC	2 cut(s)	348, 484
PspPI	GGNCC	3 cut(s)	28, 227, 346
PspPPI	RGGWCCY	1 cut(s)	346
PsuI	RGATCY	2 cut(s)	36, 1006
RsaI	GTAC	1 cut(s)	249
RsaNI	GTAC	1 cut(s)	248
RseI	CAYNNNNRTG	1 cut(s)	665
SapI	GCTCTTC	1 cut(s)	1130
SaqAI	TTAA	3 cut(s)	567, 651, 705
SatI	GCNGC	3 cut(s)	108, 858, 1141
Sau3AI	GATC	7 cut(s)	12, 36, 167, 926, 1006, 1015, 1087
Sau96I	GGNCC	3 cut(s)	28, 227, 346
SfaNI	GCATC	3 cut(s)	228, 650, 715
SfiI	GGCCNNNNNGGCC	1 cut(s)	107
SinI	GGWCC	2 cut(s)	227, 346
SmiMI	CAYNNNNRTG	1 cut(s)	665
SsiI	CCGC	2 cut(s)	107, 165
SspI	AATATT	2 cut(s)	450, 766
SspMI	CTAG	1 cut(s)	605
StyI	CCWWGG	2 cut(s)	178, 543
TaaI	ACNGT	3 cut(s)	64, 255, 950
TaiI	ACGT	1 cut(s)	1219
TaqI	TCGA	1 cut(s)	42
TauI	GCSGC	1 cut(s)	110
TfiI	GAWTC	2 cut(s)	625, 1165
Tru1I	TTAA	3 cut(s)	567, 651, 705
Tru9I	TTAA	3 cut(s)	567, 651, 705
TscAI	CASTG	2 cut(s)	153, 462
TseFI	GTSAC	1 cut(s)	1241
TseI	GCWGC	2 cut(s)	857, 1140
Tsp45I	GTSAC	1 cut(s)	1241
TspDTI	ATGAA	4 cut(s)	156, 423, 677, 759
TspGWI	ACGGA	1 cut(s)	939
TspRI	CASTG	2 cut(s)	153, 462
VpaK11BI	GGWCC	2 cut(s)	227, 346
XagI	CCTNNNNNAGG	1 cut(s)	182
XapI	RAATTY	3 cut(s)	617, 866, 901
XspI	CTAG	1 cut(s)	605

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.