Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr1

Orthogroup ID

OG0002577

Physical Location & Seq

Genomic Coordinates Reverse (-)

63207780 .. 63210784

Gene Structure 3005 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw1G035410.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1326 bp

ATGGCGACCACGATCAAGCTCATCAAGGCCCGTCAGATCTTCGACAGCCGTGGAAATCCCACCGTTGAAGTTGATGTTACTCTTTCCGATGGAACTCTGGCCAGAGCGGCCGTGCCGAGTGGAGCTTCCACAGGTGTATATGAAGCACTGGAGTTGAGAGATGGCGGATCGGATTACCTTGGGAAGGGTGTCCTGAAGGCTGTGGAGAATGTGAATGGCATCATTGGACCTGCCTTGATTGGAAAGACCCAAATTGACAATTATATGGTTCAACAGCTTGATGGAACTACAAATGAATGGGGTTGGTGCAAACAGAAGCTTGGAGCAAATGCAATATTGGCAGTGTCTCTTGCTGTTTGTAAAGCAGGTGCCATTGTGAAAAAGATACCGCTATACAAGCACATAGCCAATCTTGCTGGAAACAAGACCTTGGTTCTGCCTGTTCCTGCATTTAATGTCATCAATGGAGGTTCTCATGCTGGCAATAAACTAGCCATGCAGGAATTTATGATTCTCCCTGTTGGAGCATCTTCTTTTAAGGAAGCCATGAAGATGGGTGTAGAAGTATATCATCATCTGAAGGCTGTAATTAAGAAGAAGTATGGACAAGATGCCACAAATGTTGGGGATGAAGGTGGCTTTGCACCCAATATTCAGGAAAACAAAGAGGGTCTTGAACTTCTGAAGACAGCTATTGCTAAAGCTGGATATACCGGAAAGGTTGTTATTGGGATGGATGTTGCTGCTTCAGAATTCTATGATGATAAGGATAAGACTTATGATTTGAATTTCAAGGAAGAGAAAAATGACGGATCACAAAGAATATCAGGAGACAGTCTGAAGAATGTTTACAAGTCATTTGTTACAGAGTATCCAATTGTGTCAATTGAAGATCCCTTTGATCAGGATGACTGGGAACACTATGCTAAGATGACTGGTGAAATTGGCGAGCAAGTGCAAATTGTTGGTGATGATCTCCTTGTTACAAACCCAAAGCGAGTGGAGAAAGCAATCAAGGAGAAGAGCTGCAATGCCCTTCTTTTGAAGGTGAATCAAATTGGTTCAGTAACCGAAAGTATTGAAGCTGTGAAAATGTCAAAACGTGCTGGATGGGGTGTCATGGCAAGTCACCGAAGTGGTGAAACTGAGGATACTTTCATTGCTGACCTTTCTGTGGGGCTTGCAACGGGCCAGATTAAGACTGGAGCTCCATGCAGATCCGAGCGTCTGGCTAAGTACAACCAGCTTCTTAGAATTGAAGAGGAGCTTGGGCCTGCAGCAGTCTATGCTGGATCAAAATTCAGAGTACCAGTGGAGCCATACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00010 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko03018 ko04066 map00010 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map03018 map04066

Pfam Domains

Protein Families

Enolase_C Enolase_N

Protein Analysis

441

Amino Acids

47.64

Weight (kDa)

6.17

Isoelectric Point (pI)

35.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Enolase_N	PF03952	5 - 135	6.6e-48	Enolase, N-terminal domain
Enolase_C	PF00113	144 - 435	1.5e-158	Enolase, C-terminal TIM barrel domain
MR_MLE_C	PF13378	270 - 403	2.9e-08	Enolase C-terminal domain-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002577)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G36530 AT2G36530
fragaria_vesca	FvH4_6g17770 FvH4_6g17770 FvH4_7g28150
malus_domestica	MD01G1185500.v1.1 MD12G1016600.v1.1 MD14G1014600.v1.1
prunus_persica	Prupe.2G281900_v2.0.a1 Prupe.2G281900_v2.0.a1 Prupe.7G118700_v2.0.a1
pyrus_communis	pycom01g19710 pycom12g01360 pycom14g01220
rosa_chinensis	RchiOBHm_Chr1g0375091 RchiOBHm_Chr3g0472001
rosa_laevigata	RLG00000000873 RLG00000024113
rosa_multiflora	Rmu_sc0000090.1_g000028 Rmu_sc0000554.1_g000017 Rmu_sc0002532.1_g000001 Rmu_sc0034562.1_g000001 Rmu_sc0039689.1_g000001
rosa_roxburghii	Rroxscaffold_4G00283020 Rroxscaffold_6G00409370
rosa_rugosa	Rorug01G0388300 Rorug01G0388400 Rorug03G0121500 Rorug03G0121600
rosa_samantha	Rh1AG398800 Rh1BG365700 Rh1CG378600 Rh1DG396400 Rh3AG173200 Rh3BG198600 Rh3CG189800 Rh3CG197100 Rh3DG175800
rosa_wichuraiana	Rw1G035410 Rw3G016750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	356
Acc36I	ACCTGC	2 cut(s)	238, 356
AccB1I	GGYRCC	1 cut(s)	368
AccBSI	CCGCTC	1 cut(s)	107
AciI	CCGC	3 cut(s)	107, 165, 389
AclWI	GGATC	5 cut(s)	175, 820, 887, 1212, 1300
AcoI	YGGCCR	2 cut(s)	99, 108
AcsI	RAATTY	4 cut(s)	503, 752, 787, 1298
AcuI	CTGAAG	5 cut(s)	215, 599, 704, 732, 860
AfaI	GTAC	2 cut(s)	1238, 1308
AfiI	CCNNNNNNNGG	2 cut(s)	184, 1174
AgsI	TTSAA	9 cut(s)	68, 272, 677, 787, 793, 890, 1045, 1082, 1259
AjuI	GAANNNNNNNTTGG	2 cut(s)	1251, 1283
AleI	CACNNNNGTG	1 cut(s)	1134
Alw21I	GWGCWC	1 cut(s)	1210
Alw26I	GTCTC	2 cut(s)	351, 825
AlwI	GGATC	5 cut(s)	175, 820, 887, 1212, 1300
AoxI	GGCC	5 cut(s)	27, 99, 108, 1189, 1271
ApeKI	GCWGC	3 cut(s)	743, 1026, 1277
ApoI	RAATTY	4 cut(s)	503, 752, 787, 1298
ArsI	GACNNNNNNTTYG	2 cut(s)	766, 798
AspS9I	GGNCC	4 cut(s)	28, 227, 1189, 1271
AsuHPI	GGTGA	5 cut(s)	950, 980, 1060, 1121, 1151
AvaII	GGWCC	1 cut(s)	227
BalI	TGGCCA	1 cut(s)	101
BanI	GGYRCC	1 cut(s)	368
BanII	GRGCYC	1 cut(s)	1210
BarI	GAAGNNNNNNTAC	2 cut(s)	833, 865
BbsI	GAAGAC	1 cut(s)	692
Bbv12I	GWGCWC	1 cut(s)	1210
BbvI	GCAGC	3 cut(s)	730, 1013, 1289
BccI	CCATC	6 cut(s)	83, 155, 275, 547, 727, 1104
BceAI	ACGGC	2 cut(s)	33, 95
BciVI	GTATCC	2 cut(s)	882, 1144
BclI	TGATCA	1 cut(s)	901
BcoDI	GTCTC	2 cut(s)	351, 825
BfaI	CTAG	1 cut(s)	491
BfmI	CTRYAG	1 cut(s)	1275
BfuAI	ACCTGC	2 cut(s)	238, 356
BfuI	GTATCC	2 cut(s)	882, 1144
BglI	GCCNNNNNGGC	1 cut(s)	107
BglII	AGATCT	1 cut(s)	36
BisI	GCNGC	4 cut(s)	108, 744, 1027, 1278
BlsI	GCNGC	4 cut(s)	109, 745, 1028, 1279
Bme18I	GGWCC	1 cut(s)	227
BmgT120I	GGNCC	4 cut(s)	28, 227, 1189, 1271
BmiI	GGNNCC	2 cut(s)	370, 1317
BmrI	ACTGGG	1 cut(s)	922
BmsI	GCATC	3 cut(s)	228, 536, 601
BmuI	ACTGGG	1 cut(s)	922
BpiI	GAAGAC	1 cut(s)	692
BpmI	CTGGAG	2 cut(s)	170, 1224
BsaJI	CCNNGG	3 cut(s)	49, 178, 429
BsaWI	WCCGGW	1 cut(s)	713
BsaXI	ACNNNNNCTCC	2 cut(s)	1192, 1222
Bsc4I	CCNNNNNNNGG	2 cut(s)	184, 1174
Bse1I	ACTGG	5 cut(s)	153, 917, 940, 1207, 1310
Bse3DI	GCAATG	2 cut(s)	1036, 1158
BseDI	CCNNGG	3 cut(s)	49, 178, 429
BseGI	GGATG	5 cut(s)	634, 738, 742, 913, 1115
BseLI	CCNNNNNNNGG	2 cut(s)	184, 1174
BseMI	GCAATG	2 cut(s)	1036, 1158
BseMII	CTCAG	1 cut(s)	1137
BseNI	ACTGG	5 cut(s)	153, 917, 940, 1207, 1310
BseRI	GAGGAG	1 cut(s)	1277
BseX3I	CGGCCG	1 cut(s)	108
BseXI	GCAGC	3 cut(s)	730, 1013, 1289
Bsh1285I	CGRYCG	1 cut(s)	111
BshFI	GGCC	5 cut(s)	29, 101, 110, 1191, 1273
BshNI	GGYRCC	1 cut(s)	368
BsiEI	CGRYCG	1 cut(s)	111
BsiHKAI	GWGCWC	1 cut(s)	1210
BsiSI	CCGG	1 cut(s)	714
BslI	CCNNNNNNNGG	2 cut(s)	184, 1174
BsmAI	GTCTC	2 cut(s)	351, 825
BsnI	GGCC	5 cut(s)	29, 101, 110, 1191, 1273
Bsp1286I	GDGCHC	1 cut(s)	1210
Bsp143I	GATC	9 cut(s)	12, 36, 167, 812, 892, 901, 973, 1217, 1292
BspACI	CCGC	3 cut(s)	107, 165, 389
BspANI	GGCC	5 cut(s)	29, 101, 110, 1191, 1273
BspCNI	CTCAG	1 cut(s)	1138
BspLI	GGNNCC	2 cut(s)	370, 1317
BspMAI	CTGCAG	1 cut(s)	1279
BspMI	ACCTGC	2 cut(s)	238, 356
BspPI	GGATC	5 cut(s)	175, 820, 887, 1212, 1300
BspQI	GCTCTTC	1 cut(s)	1016
BspT107I	GGYRCC	1 cut(s)	368
BsrBI	CCGCTC	1 cut(s)	107
BsrDI	GCAATG	2 cut(s)	1036, 1158
BsrI	ACTGG	5 cut(s)	153, 917, 940, 1207, 1310
BssECI	CCNNGG	3 cut(s)	49, 178, 429
BssMI	GATC	9 cut(s)	12, 36, 167, 812, 892, 901, 973, 1217, 1292
BssT1I	CCWWGG	2 cut(s)	178, 429
Bst4CI	ACNGT	2 cut(s)	64, 836
Bst6I	CTCTTC	3 cut(s)	792, 1016, 1254
BstAPI	GCANNNNNTGC	1 cut(s)	1286
BstC8I	GCNNGC	4 cut(s)	481, 950, 1182, 1275
BstDEI	CTNAG	4 cut(s)	927, 1146, 1233, 1250
BstDSI	CCRYGG	1 cut(s)	49
BstENI	CCTNNNNNAGG	1 cut(s)	182
BstF5I	GGATG	5 cut(s)	634, 738, 742, 913, 1115
BstKTI	GATC	9 cut(s)	15, 39, 170, 815, 895, 904, 976, 1220, 1295
BstMAI	GTCTC	2 cut(s)	351, 825
BstMBI	GATC	9 cut(s)	12, 36, 167, 812, 892, 901, 973, 1217, 1292
BstMCI	CGRYCG	1 cut(s)	111
BstMWI	GCNNNNNNNGC	5 cut(s)	107, 338, 397, 413, 1286
BstSFI	CTRYAG	1 cut(s)	1275
BstV1I	GCAGC	3 cut(s)	730, 1013, 1289
BstV2I	GAAGAC	1 cut(s)	692
BstX2I	RGATCY	3 cut(s)	36, 892, 1217
BstXI	CCANNNNNNTGG	1 cut(s)	553
BstYI	RGATCY	3 cut(s)	36, 892, 1217
BstZI	CGGCCG	1 cut(s)	108
BsuI	GTATCC	2 cut(s)	882, 1144
BsuRI	GGCC	5 cut(s)	29, 101, 110, 1191, 1273
BtgI	CCRYGG	1 cut(s)	49
BtsCI	GGATG	5 cut(s)	634, 738, 742, 913, 1115
BtsI	GCAGTG	1 cut(s)	348
BtsIMutI	CAGTG	3 cut(s)	146, 348, 1317
BveI	ACCTGC	2 cut(s)	238, 356
Cac8I	GCNNGC	4 cut(s)	481, 950, 1182, 1275
Cfr13I	GGNCC	4 cut(s)	28, 227, 1189, 1271
CseI	GACGC	1 cut(s)	1214
Csp6I	GTAC	2 cut(s)	1237, 1307
CspCI	CAANNNNNGTGG	4 cut(s)	604, 639, 981, 1016
CviAII	CATG	5 cut(s)	476, 496, 547, 1120, 1212
CviQI	GTAC	2 cut(s)	1237, 1307
DdeI	CTNAG	4 cut(s)	927, 1146, 1233, 1250
DpnI	GATC	9 cut(s)	14, 38, 169, 814, 894, 903, 975, 1219, 1294
DpnII	GATC	9 cut(s)	12, 36, 167, 812, 892, 901, 973, 1217, 1292
EaeI	YGGCCR	2 cut(s)	99, 108
EagI	CGGCCG	1 cut(s)	108
Eam1104I	CTCTTC	3 cut(s)	792, 1016, 1254
EarI	CTCTTC	3 cut(s)	792, 1016, 1254
EciI	GGCGGA	1 cut(s)	180
Ecl136II	GAGCTC	1 cut(s)	1208
EclXI	CGGCCG	1 cut(s)	108
Eco130I	CCWWGG	2 cut(s)	178, 429
Eco24I	GRGCYC	1 cut(s)	1210
Eco47I	GGWCC	1 cut(s)	227
Eco52I	CGGCCG	1 cut(s)	108
Eco53kI	GAGCTC	1 cut(s)	1208
Eco57I	CTGAAG	5 cut(s)	215, 599, 704, 732, 860
EcoICRI	GAGCTC	1 cut(s)	1208
EcoNI	CCTNNNNNAGG	1 cut(s)	182
EcoRI	GAATTC	1 cut(s)	752
EcoT14I	CCWWGG	2 cut(s)	178, 429
EcoT38I	GRGCYC	1 cut(s)	1210
ErhI	CCWWGG	2 cut(s)	178, 429
FaeI	CATG	5 cut(s)	479, 499, 550, 1123, 1215
FalI	AAGNNNNNCTT	4 cut(s)	657, 689, 1251, 1283
FatI	CATG	5 cut(s)	475, 495, 546, 1119, 1211
FbaI	TGATCA	1 cut(s)	901
Fnu4HI	GCNGC	4 cut(s)	108, 744, 1027, 1278
FokI	GGATG	5 cut(s)	641, 745, 749, 920, 1122
FriOI	GRGCYC	1 cut(s)	1210
Fsp4HI	GCNGC	4 cut(s)	108, 744, 1027, 1278
FspBI	CTAG	1 cut(s)	491
GluI	GCNGC	4 cut(s)	108, 744, 1027, 1278
GsuI	CTGGAG	2 cut(s)	170, 1224
HaeIII	GGCC	5 cut(s)	29, 101, 110, 1191, 1273
HapII	CCGG	1 cut(s)	714
HgaI	GACGC	1 cut(s)	1214
Hin1II	CATG	5 cut(s)	479, 499, 550, 1123, 1215
HindIII	AAGCTT	1 cut(s)	317
HinfI	GANTC	2 cut(s)	511, 1051
HpaII	CCGG	1 cut(s)	714
HphI	GGTGA	5 cut(s)	950, 980, 1060, 1121, 1151
Hpy166II	GTNNAC	1 cut(s)	850
Hpy188I	TCNGA	9 cut(s)	36, 88, 172, 579, 684, 751, 840, 1222, 1304
Hpy188III	TCNNGA	5 cut(s)	193, 656, 674, 828, 905
Hpy8I	GTNNAC	1 cut(s)	850
HpyAV	CCTTC	6 cut(s)	178, 190, 574, 626, 1039, 1046
HpyCH4III	ACNGT	2 cut(s)	64, 836
HpyCH4IV	ACGT	1 cut(s)	1102
HpyF10VI	GCNNNNNNNGC	5 cut(s)	107, 338, 397, 413, 1286
HpyF3I	CTNAG	4 cut(s)	927, 1146, 1233, 1250
HpySE526I	ACGT	1 cut(s)	1102
Hsp92II	CATG	5 cut(s)	479, 499, 550, 1123, 1215
Ksp22I	TGATCA	1 cut(s)	901
Kzo9I	GATC	9 cut(s)	12, 36, 167, 812, 892, 901, 973, 1217, 1292
LguI	GCTCTTC	1 cut(s)	1016
LmnI	GCTCC	7 cut(s)	122, 323, 524, 1205, 1213, 1264, 1315
Lsp1109I	GCAGC	3 cut(s)	730, 1013, 1289
LweI	GCATC	3 cut(s)	228, 536, 601
MaeI	CTAG	1 cut(s)	491
MaeII	ACGT	1 cut(s)	1102
MaeIII	GTNAC	5 cut(s)	76, 862, 982, 1066, 1127
MalI	GATC	9 cut(s)	14, 38, 169, 814, 894, 903, 975, 1219, 1294
MbiI	CCGCTC	1 cut(s)	107
MboI	GATC	9 cut(s)	12, 36, 167, 812, 892, 901, 973, 1217, 1292
MfeI	CAATTG	2 cut(s)	876, 885
MflI	RGATCY	3 cut(s)	36, 892, 1217
MhlI	GDGCHC	1 cut(s)	1210
MlsI	TGGCCA	1 cut(s)	101
MluNI	TGGCCA	1 cut(s)	101
MmeI	TCCRAC	1 cut(s)	502
MnlI	CCTC	4 cut(s)	461, 661, 1141, 1255
Mox20I	TGGCCA	1 cut(s)	101
MscI	TGGCCA	1 cut(s)	101
MseI	TTAA	4 cut(s)	453, 537, 591, 1197
MslI	CAYNNNNRTG	2 cut(s)	551, 1134
Msp20I	TGGCCA	1 cut(s)	101
MspI	CCGG	1 cut(s)	714
MunI	CAATTG	2 cut(s)	876, 885
MwoI	GCNNNNNNNGC	5 cut(s)	107, 338, 397, 413, 1286
NdeII	GATC	9 cut(s)	12, 36, 167, 812, 892, 901, 973, 1217, 1292
NlaIII	CATG	5 cut(s)	479, 499, 550, 1123, 1215
NlaIV	GGNNCC	2 cut(s)	370, 1317
NmeAIII	GCCGAG	1 cut(s)	141
NmuCI	GTSAC	1 cut(s)	1127
OliI	CACNNNNGTG	1 cut(s)	1134
PaqCI	CACCTGC	1 cut(s)	356
PciSI	GCTCTTC	1 cut(s)	1016
PfeI	GAWTC	2 cut(s)	511, 1051
PkrI	GCNGC	4 cut(s)	109, 745, 1028, 1279
Psp124BI	GAGCTC	1 cut(s)	1210
PspN4I	GGNNCC	2 cut(s)	370, 1317
PspPI	GGNCC	4 cut(s)	28, 227, 1189, 1271
PstI	CTGCAG	1 cut(s)	1279
PsuI	RGATCY	3 cut(s)	36, 892, 1217
RsaI	GTAC	2 cut(s)	1238, 1308
RsaNI	GTAC	2 cut(s)	1237, 1307
RseI	CAYNNNNRTG	2 cut(s)	551, 1134
SacI	GAGCTC	1 cut(s)	1210
SapI	GCTCTTC	1 cut(s)	1016
SaqAI	TTAA	4 cut(s)	453, 537, 591, 1197
SatI	GCNGC	4 cut(s)	108, 744, 1027, 1278
Sau3AI	GATC	9 cut(s)	12, 36, 167, 812, 892, 901, 973, 1217, 1292
Sau96I	GGNCC	4 cut(s)	28, 227, 1189, 1271
SduI	GDGCHC	1 cut(s)	1210
SfaNI	GCATC	3 cut(s)	228, 536, 601
SfcI	CTRYAG	1 cut(s)	1275
SfiI	GGCCNNNNNGGCC	1 cut(s)	107
SinI	GGWCC	1 cut(s)	227
SmiMI	CAYNNNNRTG	2 cut(s)	551, 1134
SsiI	CCGC	3 cut(s)	107, 165, 389
SspI	AATATT	2 cut(s)	336, 652
SspMI	CTAG	1 cut(s)	491
SstI	GAGCTC	1 cut(s)	1210
StyI	CCWWGG	2 cut(s)	178, 429
TaaI	ACNGT	2 cut(s)	64, 836
TaiI	ACGT	1 cut(s)	1105
TaqI	TCGA	1 cut(s)	42
TatI	WGTACW	1 cut(s)	1236
TauI	GCSGC	1 cut(s)	110
TfiI	GAWTC	2 cut(s)	511, 1051
Tru1I	TTAA	4 cut(s)	453, 537, 591, 1197
Tru9I	TTAA	4 cut(s)	453, 537, 591, 1197
TscAI	CASTG	3 cut(s)	153, 348, 1317
TseFI	GTSAC	1 cut(s)	1127
TseI	GCWGC	3 cut(s)	743, 1026, 1277
Tsp45I	GTSAC	1 cut(s)	1127
TspDTI	ATGAA	5 cut(s)	156, 309, 563, 645, 1147
TspGWI	ACGGA	1 cut(s)	825
TspRI	CASTG	3 cut(s)	153, 348, 1317
VpaK11BI	GGWCC	1 cut(s)	227
XagI	CCTNNNNNAGG	1 cut(s)	182
XapI	RAATTY	4 cut(s)	503, 752, 787, 1298
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1199
XspI	CTAG	1 cut(s)	491

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.