Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0002577

Physical Location & Seq

Genomic Coordinates Forward (+)

1287135 .. 1291116

Gene Structure 3982 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1014600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1335 bp

ATGGCGACGATCCAGAGCGTCAAGGCTAGGCAGATCTTCGACAGCCGTGGCAATCCCACCGTCGAGGTCGATATCATACTGTCAGATGGCACTTTGGCTAGAGCCGCTGTTCCAAGTGGTGCATCCACTGGAGTTTACGAGGCCCTTGAGTTGCGCGATGGAGGCAAAGACTACCTCGGAAAGGGTGTTTCGAAGGCTGTCAACAATGTCAACACAATCATTGGCCCCGCTTTGATCGGCAAGGACCCGAGCGAACAGACGGCTATTGACAACTTCATGGTTCAACAGCTCGATGGAACCGTCAACGAGTGGGGTTGGTGCAAGCAAAAGCTTGGTGCAAATGCCATTTTGGCCGTGTCTCTTGCAGTCGCCAAGGCTGGTGCTAGCGTCAAGAAAGTTCCTCTCTACAAGCATATCGCCAATCTTGCTGGCAACAAGAACCTAGTGTTGCCCGTTCCTGCTTTCAATGTCATCAACGGTGGATCACATGCAGGAAACAAGCTTGCAATGCAGGAGTTCATGATTCTTCCTATTGGAGCTTCCTCATTCAAGGAAGCCATGAAGATGGGTGTGGAAGTATATCACAACCTTAAGTCTGTGATTAAAAAGAAGTACGGTCAGGATGCAACCAATGTTGGTGATGAAGGTGGATTTGCTCCTAACATCCAGGAGAACAGGGAAGGTCTTGAATTACTCAAGACAGCCATCGAGAAGGCTGGATACACCGGCAAAGTTGTCATTGGAATGGATGTTGCTGCATCTGAGTTTTATGGATCAGATAAAACCTATGACCTGAACTTCAAGGAAGAGAAAAATGATGGTTCCCAAAAGATCTCAGGAAATGCTCTCAAGGACCTCTACAAGTCATTTGCAAGTGAGTACCCAATTGTTTCAATTGAAGATCCATTTGACCAAGATGACTGGGAGCACTATGCCAAGATGACAGCTGAAGTTGGAGAAAAAATACAAATTGTGGGAGATGATCTGTTGGTGACCAACCCCAAGAGAGTCGAGAAGGCAATCAAGGAAAAATCTTGCAATGCTCTTCTTCTCAAGGTGAATCAAATTGGATCAGTAACCGAGAGTATTGAGGCTGTAAGAATGTCCAAGAAAGCTGGATGGGGTGTGATGACCAGCCACCGCAGTGGAGAAACCGAGGACACCTTCATTGCTGATCTCGCTGTTGGTTTGGCTACGGGACAAATCAAGACAGGAGCTCCCTGCAGGTCGGAGCGTCTTGCCAAGTACAACCAGCTGTTGCGAATTGAGGAGGAGCTCGGTGCTGAGGCAGTGTATGCCGGAGCTAAGTTCCGCGTGCCCGTTGAGCCCTATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004634 GO:0005488 GO:0005507 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005740 GO:0005829 GO:0005886 GO:0005911 GO:0006950 GO:0006970 GO:0008150 GO:0009266 GO:0009314 GO:0009409 GO:0009416 GO:0009506 GO:0009507 GO:0009536 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0010033 GO:0010035 GO:0010038 GO:0016020 GO:0016829 GO:0016835 GO:0016836 GO:0030054 GO:0031967 GO:0031975 GO:0033993 GO:0042221 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0046686 GO:0046872 GO:0046914 GO:0048046 GO:0050896 GO:0055044 GO:0071944 GO:0097159 GO:0097305 GO:1901363 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00010 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko03018 ko04066 map00010 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map03018 map04066

Pfam Domains

Protein Families

Enolase_C Enolase_N

Protein Analysis

445

Amino Acids

47.84

Weight (kDa)

5.92

Isoelectric Point (pI)

35.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Enolase_N	PF03952	4 - 139	1.8e-53	Enolase, N-terminal domain
Enolase_C	PF00113	148 - 438	1.2e-158	Enolase, C-terminal TIM barrel domain
MR_MLE_C	PF13378	279 - 404	1.8e-08	Enolase C-terminal domain-like
MAAL_C	PF07476	293 - 401	5.4e-06	Methylaspartate ammonia-lyase C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002577)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G36530 AT2G36530
fragaria_vesca	FvH4_6g17770 FvH4_6g17770 FvH4_7g28150
malus_domestica	MD01G1185500.v1.1 MD12G1016600.v1.1 MD14G1014600.v1.1
prunus_persica	Prupe.2G281900_v2.0.a1 Prupe.2G281900_v2.0.a1 Prupe.7G118700_v2.0.a1
pyrus_communis	pycom01g19710 pycom12g01360 pycom14g01220
rosa_chinensis	RchiOBHm_Chr1g0375091 RchiOBHm_Chr3g0472001
rosa_laevigata	RLG00000000873 RLG00000024113
rosa_multiflora	Rmu_sc0000090.1_g000028 Rmu_sc0000554.1_g000017 Rmu_sc0002532.1_g000001 Rmu_sc0034562.1_g000001 Rmu_sc0039689.1_g000001
rosa_roxburghii	Rroxscaffold_4G00283020 Rroxscaffold_6G00409370
rosa_rugosa	Rorug01G0388300 Rorug01G0388400 Rorug03G0121500 Rorug03G0121600
rosa_samantha	Rh1AG398800 Rh1BG365700 Rh1CG378600 Rh1DG396400 Rh3AG173200 Rh3BG198600 Rh3CG189800 Rh3CG197100 Rh3DG175800
rosa_wichuraiana	Rw1G035410 Rw3G016750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1215
AccII	CGCG	2 cut(s)	156, 1314
AciI	CCGC	4 cut(s)	105, 228, 1141, 1312
AclWI	GGATC	5 cut(s)	4, 490, 781, 896, 1078
AcoI	YGGCCR	1 cut(s)	351
AcuI	CTGAAG	1 cut(s)	969
AfaI	GTAC	3 cut(s)	614, 881, 1247
AfiI	CCNNNNNNNGG	1 cut(s)	181
AflII	CTTAAG	1 cut(s)	590
AgsI	TTSAA	7 cut(s)	284, 466, 550, 689, 802, 894, 899
AjnI	CCWGG	1 cut(s)	666
AleI	CACNNNNGTG	1 cut(s)	1143
Alw21I	GWGCWC	3 cut(s)	930, 1219, 1278
Alw26I	GTCTC	1 cut(s)	363
AlwI	GGATC	5 cut(s)	4, 490, 781, 896, 1078
Ama87I	CYCGRG	1 cut(s)	247
AoxI	GGCC	3 cut(s)	141, 223, 351
ApeKI	GCWGC	1 cut(s)	755
AspLEI	GCGC	1 cut(s)	156
AspS9I	GGNCC	4 cut(s)	142, 224, 244, 853
AsuHPI	GGTGA	3 cut(s)	650, 1003, 1069
AsuII	TTCGAA	1 cut(s)	191
AsuNHI	GCTAGC	1 cut(s)	383
AvaI	CYCGRG	1 cut(s)	247
AvaII	GGWCC	2 cut(s)	244, 853
BaeGI	GKGCMC	1 cut(s)	1320
BanII	GRGCYC	3 cut(s)	1219, 1278, 1329
BarI	GAAGNNNNNNTAC	2 cut(s)	704, 736
Bbv12I	GWGCWC	3 cut(s)	930, 1219, 1278
BbvCI	CCTCAGC	1 cut(s)	1284
BbvI	GCAGC	1 cut(s)	742
BccI	CCATC	7 cut(s)	80, 152, 287, 559, 713, 812, 1113
BceAI	ACGGC	3 cut(s)	30, 276, 338
BciT130I	CCWGG	1 cut(s)	668
BciVI	GTATCC	1 cut(s)	713
BcoDI	GTCTC	1 cut(s)	363
BfaI	CTAG	4 cut(s)	27, 99, 384, 443
BfmI	CTRYAG	1 cut(s)	1222
BfrI	CTTAAG	1 cut(s)	590
BfuAI	ACCTGC	1 cut(s)	1215
BfuI	GTATCC	1 cut(s)	713
BglI	GCCNNNNNGGC	1 cut(s)	350
BglII	AGATCT	2 cut(s)	33, 831
BisI	GCNGC	2 cut(s)	105, 756
BlsI	GCNGC	2 cut(s)	106, 757
Bme1390I	CCNGG	1 cut(s)	668
Bme18I	GGWCC	2 cut(s)	244, 853
BmeT110I	CYCGRG	1 cut(s)	247
BmgT120I	GGNCC	4 cut(s)	142, 224, 244, 853
BmiI	GGNNCC	4 cut(s)	226, 246, 298, 823
BmrFI	CCNGG	1 cut(s)	668
BmrI	ACTGGG	1 cut(s)	931
BmsI	GCATC	3 cut(s)	131, 613, 767
BmtI	GCTAGC	1 cut(s)	387
BmuI	ACTGGG	1 cut(s)	931
BpmI	CTGGAG	1 cut(s)	150
Bpu10I	CCTNAGC	1 cut(s)	1284
Bpu14I	TTCGAA	1 cut(s)	191
BpuEI	CTTGAG	4 cut(s)	167, 680, 833, 1037
BsaJI	CCNNGG	4 cut(s)	46, 175, 372, 1155
BsaXI	ACNNNNNCTCC	2 cut(s)	1201, 1231
Bsc4I	CCNNNNNNNGG	1 cut(s)	181
Bse118I	RCCGGY	1 cut(s)	725
Bse1I	ACTGG	2 cut(s)	133, 926
Bse3DI	GCAATG	3 cut(s)	513, 1045, 1167
BseBI	CCWGG	1 cut(s)	668
BseDI	CCNNGG	4 cut(s)	46, 175, 372, 1155
BseGI	GGATG	5 cut(s)	122, 628, 663, 754, 1124
BseLI	CCNNNNNNNGG	1 cut(s)	181
BseMI	GCAATG	3 cut(s)	513, 1045, 1167
BseMII	CTCAG	3 cut(s)	753, 849, 1275
BseNI	ACTGG	2 cut(s)	133, 926
BseRI	GAGGAG	2 cut(s)	1283, 1286
BseSI	GKGCMC	1 cut(s)	1320
BseXI	GCAGC	1 cut(s)	742
Bsh1236I	CGCG	2 cut(s)	156, 1314
BshFI	GGCC	3 cut(s)	143, 225, 353
BsiHKAI	GWGCWC	3 cut(s)	930, 1219, 1278
BsiHKCI	CYCGRG	1 cut(s)	247
BsiSI	CCGG	2 cut(s)	726, 1299
BslFI	GGGAC	1 cut(s)	1212
BslI	CCNNNNNNNGG	1 cut(s)	181
BsmAI	GTCTC	1 cut(s)	363
BsmFI	GGGAC	1 cut(s)	1212
BsnI	GGCC	3 cut(s)	143, 225, 353
BsoBI	CYCGRG	1 cut(s)	247
Bsp119I	TTCGAA	1 cut(s)	191
Bsp1286I	GDGCHC	5 cut(s)	930, 1219, 1278, 1320, 1329
BspACI	CCGC	4 cut(s)	105, 228, 1141, 1312
BspANI	GGCC	3 cut(s)	143, 225, 353
BspCNI	CTCAG	3 cut(s)	754, 848, 1276
BspFNI	CGCG	2 cut(s)	156, 1314
BspHI	TCATGA	1 cut(s)	519
BspLI	GGNNCC	4 cut(s)	226, 246, 298, 823
BspMAI	CTGCAG	1 cut(s)	1226
BspMI	ACCTGC	1 cut(s)	1215
BspOI	GCTAGC	1 cut(s)	387
BspPI	GGATC	5 cut(s)	4, 490, 781, 896, 1078
BspQI	GCTCTTC	1 cut(s)	1050
BspT104I	TTCGAA	1 cut(s)	191
BspTI	CTTAAG	1 cut(s)	590
BsrDI	GCAATG	3 cut(s)	513, 1045, 1167
BsrFI	RCCGGY	1 cut(s)	725
BsrI	ACTGG	2 cut(s)	133, 926
BssAI	RCCGGY	1 cut(s)	725
BssECI	CCNNGG	4 cut(s)	46, 175, 372, 1155
BssT1I	CCWWGG	1 cut(s)	372
Bst2UI	CCWGG	1 cut(s)	668
Bst4CI	ACNGT	5 cut(s)	61, 81, 301, 479, 617
Bst6I	CTCTTC	2 cut(s)	801, 1050
BstAFI	CTTAAG	1 cut(s)	590
BstAPI	GCANNNNNTGC	1 cut(s)	1295
BstBI	TTCGAA	1 cut(s)	191
BstC8I	GCNNGC	5 cut(s)	323, 385, 430, 504, 1316
BstDEI	CTNAG	4 cut(s)	762, 835, 1284, 1305
BstDSI	CCRYGG	1 cut(s)	46
BstEII	GGTNACC	1 cut(s)	991
BstENI	CCTNNNNNAGG	1 cut(s)	179
BstF5I	GGATG	5 cut(s)	122, 628, 663, 754, 1124
BstFNI	CGCG	2 cut(s)	156, 1314
BstHHI	GCGC	1 cut(s)	156
BstMAI	GTCTC	1 cut(s)	363
BstMWI	GCNNNNNNNGC	8 cut(s)	104, 162, 350, 425, 508, 1178, 1295, 1324
BstNI	CCWGG	1 cut(s)	668
BstNSI	RCATGY	1 cut(s)	491
BstPI	GGTNACC	1 cut(s)	991
BstSCI	CCNGG	1 cut(s)	666
BstSFI	CTRYAG	1 cut(s)	1222
BstSLI	GKGCMC	1 cut(s)	1320
BstUI	CGCG	2 cut(s)	156, 1314
BstV1I	GCAGC	1 cut(s)	742
BstX2I	RGATCY	3 cut(s)	33, 831, 901
BstXI	CCANNNNNNTGG	2 cut(s)	565, 1145
BstYI	RGATCY	3 cut(s)	33, 831, 901
BsuI	GTATCC	1 cut(s)	713
BsuRI	GGCC	3 cut(s)	143, 225, 353
BtgI	CCRYGG	1 cut(s)	46
BtgZI	GCGATG	1 cut(s)	171
BtsCI	GGATG	5 cut(s)	122, 628, 663, 754, 1124
BtsI	GCAGTG	2 cut(s)	1150, 1296
BtsIMutI	CAGTG	3 cut(s)	126, 1150, 1296
BveI	ACCTGC	1 cut(s)	1215
Cac8I	GCNNGC	5 cut(s)	323, 385, 430, 504, 1316
CciI	TCATGA	1 cut(s)	519
CfoI	GCGC	1 cut(s)	156
Cfr10I	RCCGGY	1 cut(s)	725
Cfr13I	GGNCC	4 cut(s)	142, 224, 244, 853
CseI	GACGC	3 cut(s)	7, 376, 1223
Csp6I	GTAC	3 cut(s)	613, 880, 1246
CviAII	CATG	4 cut(s)	277, 488, 520, 559
CviQI	GTAC	3 cut(s)	613, 880, 1246
DdeI	CTNAG	4 cut(s)	762, 835, 1284, 1305
EaeI	YGGCCR	1 cut(s)	351
Eam1104I	CTCTTC	2 cut(s)	801, 1050
EarI	CTCTTC	2 cut(s)	801, 1050
Ecl136II	GAGCTC	2 cut(s)	1217, 1276
Eco130I	CCWWGG	1 cut(s)	372
Eco24I	GRGCYC	3 cut(s)	1219, 1278, 1329
Eco32I	GATATC	1 cut(s)	73
Eco47I	GGWCC	2 cut(s)	244, 853
Eco53kI	GAGCTC	2 cut(s)	1217, 1276
Eco57I	CTGAAG	1 cut(s)	969
Eco88I	CYCGRG	1 cut(s)	247
Eco91I	GGTNACC	1 cut(s)	991
EcoICRI	GAGCTC	2 cut(s)	1217, 1276
EcoNI	CCTNNNNNAGG	1 cut(s)	179
EcoO109I	RGGNCCY	3 cut(s)	142, 244, 853
EcoO65I	GGTNACC	1 cut(s)	991
EcoRII	CCWGG	1 cut(s)	666
EcoRV	GATATC	1 cut(s)	73
EcoT14I	CCWWGG	1 cut(s)	372
EcoT38I	GRGCYC	3 cut(s)	1219, 1278, 1329
ErhI	CCWWGG	1 cut(s)	372
FaeI	CATG	4 cut(s)	280, 491, 523, 562
FaqI	GGGAC	1 cut(s)	1212
FatI	CATG	4 cut(s)	276, 487, 519, 558
FauI	CCCGC	1 cut(s)	235
Fnu4HI	GCNGC	2 cut(s)	105, 756
FokI	GGATG	5 cut(s)	109, 635, 650, 761, 1131
FriOI	GRGCYC	3 cut(s)	1219, 1278, 1329
Fsp4HI	GCNGC	2 cut(s)	105, 756
FspBI	CTAG	4 cut(s)	27, 99, 384, 443
GlaI	GCGC	1 cut(s)	155
GluI	GCNGC	2 cut(s)	105, 756
GsuI	CTGGAG	1 cut(s)	150
HaeIII	GGCC	3 cut(s)	143, 225, 353
HapII	CCGG	2 cut(s)	726, 1299
HgaI	GACGC	3 cut(s)	7, 376, 1223
HhaI	GCGC	1 cut(s)	156
Hin1II	CATG	4 cut(s)	280, 491, 523, 562
Hin6I	GCGC	1 cut(s)	154
HinP1I	GCGC	1 cut(s)	154
HincII	GTYRAC	3 cut(s)	202, 211, 304
HindII	GTYRAC	3 cut(s)	202, 211, 304
HindIII	AAGCTT	2 cut(s)	329, 500
HinfI	GANTC	3 cut(s)	523, 1008, 1060
HpaII	CCGG	2 cut(s)	726, 1299
HphI	GGTGA	3 cut(s)	650, 1003, 1069
Hpy166II	GTNNAC	4 cut(s)	136, 202, 211, 304
Hpy188I	TCNGA	5 cut(s)	85, 179, 763, 778, 1231
Hpy8I	GTNNAC	4 cut(s)	136, 202, 211, 304
Hpy99I	CGWCG	2 cut(s)	10, 65
HpyAV	CCTTC	6 cut(s)	187, 638, 674, 706, 1009, 1174
HpyCH4III	ACNGT	5 cut(s)	61, 81, 301, 479, 617
HpyF10VI	GCNNNNNNNGC	8 cut(s)	104, 162, 350, 425, 508, 1178, 1295, 1324
HpyF3I	CTNAG	4 cut(s)	762, 835, 1284, 1305
Hsp92II	CATG	4 cut(s)	280, 491, 523, 562
HspAI	GCGC	1 cut(s)	154
LguI	GCTCTTC	1 cut(s)	1050
LmnI	GCTCC	8 cut(s)	536, 661, 925, 1214, 1222, 1231, 1273, 1301
Lsp1109I	GCAGC	1 cut(s)	742
LweI	GCATC	3 cut(s)	131, 613, 767
MaeI	CTAG	4 cut(s)	27, 99, 384, 443
MaeIII	GTNAC	2 cut(s)	991, 1075
MboII	GAAGA	7 cut(s)	28, 518, 574, 818, 911, 1037, 1040
MfeI	CAATTG	2 cut(s)	885, 894
MflI	RGATCY	3 cut(s)	33, 831, 901
MhlI	GDGCHC	5 cut(s)	930, 1219, 1278, 1320, 1329
MluCI	AATT	6 cut(s)	689, 885, 894, 969, 1065, 1263
MlyI	GAGTC	1 cut(s)	1017
MmeI	TCCRAC	2 cut(s)	934, 1209
MseI	TTAA	2 cut(s)	591, 603
MslI	CAYNNNNRTG	3 cut(s)	563, 743, 1143
MspA1I	CMGCKG	3 cut(s)	107, 947, 1255
MspCI	CTTAAG	1 cut(s)	590
MspI	CCGG	2 cut(s)	726, 1299
MspR9I	CCNGG	1 cut(s)	668
MunI	CAATTG	2 cut(s)	885, 894
MvaI	CCWGG	1 cut(s)	668
MvnI	CGCG	2 cut(s)	156, 1314
MwoI	GCNNNNNNNGC	8 cut(s)	104, 162, 350, 425, 508, 1178, 1295, 1324
NheI	GCTAGC	1 cut(s)	383
NlaIII	CATG	4 cut(s)	280, 491, 523, 562
NlaIV	GGNNCC	4 cut(s)	226, 246, 298, 823
NmuCI	GTSAC	1 cut(s)	991
NspI	RCATGY	1 cut(s)	491
NspV	TTCGAA	1 cut(s)	191
OliI	CACNNNNGTG	1 cut(s)	1143
PagI	TCATGA	1 cut(s)	519
PciSI	GCTCTTC	1 cut(s)	1050
PcsI	WCGNNNNNNNCGW	1 cut(s)	297
PfeI	GAWTC	2 cut(s)	523, 1060
PfoI	TCCNGGA	1 cut(s)	666
PkrI	GCNGC	2 cut(s)	106, 757
PleI	GAGTC	1 cut(s)	1016
PpsI	GAGTC	1 cut(s)	1016
PpuMI	RGGWCCY	2 cut(s)	244, 853
Psp124BI	GAGCTC	2 cut(s)	1219, 1278
Psp5II	RGGWCCY	2 cut(s)	244, 853
Psp6I	CCWGG	1 cut(s)	666
PspEI	GGTNACC	1 cut(s)	991
PspGI	CCWGG	1 cut(s)	666
PspN4I	GGNNCC	4 cut(s)	226, 246, 298, 823
PspPI	GGNCC	4 cut(s)	142, 224, 244, 853
PspPPI	RGGWCCY	2 cut(s)	244, 853
PstI	CTGCAG	1 cut(s)	1226
PsuI	RGATCY	3 cut(s)	33, 831, 901
PvuII	CAGCTG	2 cut(s)	947, 1255
RsaI	GTAC	3 cut(s)	614, 881, 1247
RsaNI	GTAC	3 cut(s)	613, 880, 1246
RseI	CAYNNNNRTG	3 cut(s)	563, 743, 1143
SacI	GAGCTC	2 cut(s)	1219, 1278
SapI	GCTCTTC	1 cut(s)	1050
SaqAI	TTAA	2 cut(s)	591, 603
SatI	GCNGC	2 cut(s)	105, 756
Sau96I	GGNCC	4 cut(s)	142, 224, 244, 853
SbfI	CCTGCAGG	1 cut(s)	1226
SchI	GAGTC	1 cut(s)	1017
ScrFI	CCNGG	1 cut(s)	668
SdaI	CCTGCAGG	1 cut(s)	1226
SduI	GDGCHC	5 cut(s)	930, 1219, 1278, 1320, 1329
SfaNI	GCATC	3 cut(s)	131, 613, 767
SfcI	CTRYAG	1 cut(s)	1222
SfuI	TTCGAA	1 cut(s)	191
SinI	GGWCC	2 cut(s)	244, 853
SmiMI	CAYNNNNRTG	3 cut(s)	563, 743, 1143
SmlI	CTYRAG	5 cut(s)	146, 590, 695, 848, 1052
SmoI	CTYRAG	5 cut(s)	146, 590, 695, 848, 1052
Sse8387I	CCTGCAGG	1 cut(s)	1226
Sse9I	AATT	6 cut(s)	689, 885, 894, 969, 1065, 1263
SsiI	CCGC	4 cut(s)	105, 228, 1141, 1312
SspMI	CTAG	4 cut(s)	27, 99, 384, 443
SstI	GAGCTC	2 cut(s)	1219, 1278
StyD4I	CCNGG	1 cut(s)	666
StyI	CCWWGG	1 cut(s)	372
TaaI	ACNGT	5 cut(s)	61, 81, 301, 479, 617
TaqI	TCGA	7 cut(s)	39, 63, 69, 191, 291, 708, 1011
TasI	AATT	6 cut(s)	689, 885, 894, 969, 1065, 1263
TatI	WGTACW	1 cut(s)	1245
TauI	GCSGC	1 cut(s)	107
TfiI	GAWTC	2 cut(s)	523, 1060
Tru1I	TTAA	2 cut(s)	591, 603
Tru9I	TTAA	2 cut(s)	591, 603
TscAI	CASTG	3 cut(s)	133, 1150, 1296
TseFI	GTSAC	1 cut(s)	991
TseI	GCWGC	1 cut(s)	755
Tsp45I	GTSAC	1 cut(s)	991
TspDTI	ATGAA	5 cut(s)	265, 508, 575, 657, 1156
TspRI	CASTG	3 cut(s)	133, 1150, 1296
Vha464I	CTTAAG	1 cut(s)	590
VpaK11BI	GGWCC	2 cut(s)	244, 853
XagI	CCTNNNNNAGG	1 cut(s)	179
XceI	RCATGY	1 cut(s)	491
XspI	CTAG	4 cut(s)	27, 99, 384, 443

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.