MD00G1000600.v1.1

Domain of unknown function (DUF1995)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0000465
Physical Location & Seq
Forward (+)
102003 .. 104811
2809 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1000600.v1.1.491

Sequence Viewer

Length: 966 bp
ATGCCGTTGTCGGACATCGTAACTGCCTCTAAAGTTCCGTTTGCGGCCCCTTCTCTTCCCAGTGGCAATGGCAAGAACCATTTGCCGCCAAAATCCTATGGGAATTTGGTCACCCGCAAAATGGCTGCGCAAACTGTCTCCAGAAACCTCAAATTCGTGAGGAATTCGGCTTCGGGGGATATGGAGACAAGTTCGGTTGGGCTTGATGTGTCATTTCCTAGCGACTATTCTGAGCTCATTGAACAAGCCCGAAATGCAACTGAATTGGCTGTGAAGGATAACAAGCAGTTGATGGAGATTGAGTTCCCTACGTCCGGACTTGAATCCGTACCAGGGGATGGTGAAGGAGGAATAGAAATGACAGAAAGTATGCAGTTAATTCGAGAATTTTGCAACCGTTTAATAGTTCCAGAGAAAGCTAGACAGACGAGAATATTTTTTCCCGAGGCCAACGAAGTTAAATTTGCAAGAGAATCAATATTTGATGGTGCTTCCTTTAAGTTGGACTATCTGACAAAGCCATCATTCTTTGAGGACTTTGGTTTTGTGGAGAAAGTGAAAATGATAGACCGTGTGAAGCCAGAAGATGAACTCTTCCTTGCTGCCTATCCGTATTTTAATGTCACCGAAATGCTTGTGGTCGAAGAACTTTACAGAGAAGCTGTTCAGAACACTTCCAGGAAACTGATTATTTTCAATGGGGAACTTGACCGTATAAGATCTGGATATTATCCGCCCTTCTTCTACCCGAAGCTCGCTGCACTTTCCAAGAGCCTATTTCCATTTATGGAGACTGTGTACTACATTCACAATTTTAAGGGACGCCACGGAGGGACCCTTTTTAGGAGCTACCCAGGTCCATGGAAAGTCCTCAGAAAGGTAAGGAACGGATACGTCTGTTTGCATCAGCAGGAAGCCATGCCGTCGCTCAAGGAAGTTGCTTTAGATATTCTTCCATCGGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1995

Protein Analysis

322

Amino Acids

36.32

Weight (kDa)

6.48

Isoelectric Point (pI)

45.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1995 PF09353 73 - 314 8.7e-42 Domain of unknown function (DUF1995)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000465)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48790 AT5G48790 AT5G48790
fragaria_vesca FvH4_5g23760 FvH4_5g23760 FvH4_5g23770 FvH4_5g23771 FvH4_5g23790 FvH4_5g23800 FvH4_7g03421 FvH4_7g07510
malus_domestica MD00G1000600.v1.1 MD06G1013900.v1.1
prunus_persica Prupe.2G101800_v2.0.a1 Prupe.5G022600_v2.0.a1 Prupe.5G022700_v2.0.a1
pyrus_communis pycom07g06280 pycom16g25400
rosa_chinensis RchiOBHm_Chr1g0337881 RchiOBHm_Chr1g0339341 RchiOBHm_Chr7g0213081 RchiOBHm_Chr7g0213111 RchiOBHm_Chr7g0213141 RchiOBHm_Chr7g0213151 RchiOBHm_Chr7g0213161 RchiOBHm_Chr7g0213181
rosa_laevigata RLG00000002825 RLG00000002828 RLG00000002829 RLG00000002832 RLG00000029226
rosa_multiflora Rmu_co8345885.1_g000002 Rmu_co8348513.1_g000001 Rmu_co8456259.1_g000001 Rmu_sc0000613.1_g000002 Rmu_sc0001121.1_g000005 Rmu_sc0001121.1_g000006 Rmu_sc0002989.1_g000003 Rmu_sc0015110.1_g000001 Rmu_sc0030208.1_g000001 Rmu_sc0030209.1_g000001 Rmu_sc0032877.1_g000001 Rmu_sc0039394.1_g000001 Rmu_ssc0000084.1_g000002 Rmu_ssc0000084.1_g000007 Rmu_ssc0000084.1_g000009 Rmu_ssc0000084.1_g000012
rosa_roxburghii Rroxscaffold_3G00246200 Rroxscaffold_3G00246210 Rroxscaffold_3G00246240 Rroxscaffold_3G00246250 Rroxscaffold_4G00313440
rosa_rugosa Rorug01G0142600.1 Rorug07G0139400 Rorug07G0139500 Rorug07G0139600 Rorug07G0139700.1 Rorug07G0139800 Rorug07G0140100
rosa_samantha Rh1AG159300 Rh1BG126400 Rh1CG148500 Rh7AG270700 Rh7AG270800 Rh7AG271200 Rh7AG271400 Rh7AG271500 Rh7BG266400 Rh7BG266600 Rh7BG267000 Rh7BG267100 Rh7BG267200 Rh7BG267400 Rh7CG290000 Rh7CG290100 Rh7CG290600 Rh7CG290700 Rh7CG290800 Rh7DG278900 Rh7DG279200 Rh7DG279300 Rh7DG279400 Rh7DG279500 Rh7DG279600
rosa_wichuraiana Rw1G013550 Rw7G023520 Rw7G023530 Rw7G023540 Rw7G023550 Rw7G023560 Rw7G023580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 129
AccB7I CCANNNNNTGG 1 cut(s) 338
AccIII TCCGGA 1 cut(s) 314
AciI CCGC 4 cut(s) 44, 86, 115, 734
AcsI RAATTY 5 cut(s) 103, 152, 163, 386, 461
AcyI GRCGYC 1 cut(s) 823
AfaI GTAC 2 cut(s) 330, 800
AfiI CCNNNNNNNGG 6 cut(s) 121, 314, 333, 338, 843, 877
AgsI TTSAA 3 cut(s) 242, 323, 697
AjnI CCWGG 3 cut(s) 331, 677, 853
AloI GAACNNNNNNTCC 2 cut(s) 287, 319
AluBI AGCT 5 cut(s) 235, 419, 662, 754, 849
AluI AGCT 5 cut(s) 235, 419, 662, 754, 849
Alw21I GWGCWC 1 cut(s) 237
Alw26I GTCTC 3 cut(s) 142, 179, 785
Ama87I CYCGRG 1 cut(s) 443
Aor13HI TCCGGA 1 cut(s) 314
AoxI GGCC 3 cut(s) 45, 447, 960
ApeKI GCWGC 3 cut(s) 125, 602, 758
ApoI RAATTY 5 cut(s) 103, 152, 163, 386, 461
ArsI GACNNNNNNTTYG 2 cut(s) 527, 559
Asp700I GAANNNNTTC 1 cut(s) 663
AspLEI GCGC 1 cut(s) 130
AspS9I GGNCC 3 cut(s) 46, 834, 857
AsuHPI GGTGA 3 cut(s) 103, 353, 616
AvaI CYCGRG 1 cut(s) 443
AvaII GGWCC 2 cut(s) 834, 857
BaeI ACNNNNGTAYC 2 cut(s) 883, 916
BanII GRGCYC 1 cut(s) 237
Bbv12I GWGCWC 1 cut(s) 237
BbvI GCAGC 3 cut(s) 112, 589, 745
BccI CCATC 5 cut(s) 286, 332, 479, 529, 964
BceAI ACGGC 1 cut(s) 907
BcgI CGANNNNNNTGC 4 cut(s) 362, 372, 396, 406
BciT130I CCWGG 3 cut(s) 333, 679, 855
BciVI GTATCC 1 cut(s) 884
BcoDI GTCTC 3 cut(s) 142, 179, 785
BfaI CTAG 2 cut(s) 219, 420
BfuI GTATCC 1 cut(s) 884
BglII AGATCT 1 cut(s) 719
BisI GCNGC 5 cut(s) 45, 86, 126, 603, 759
BlsI GCNGC 5 cut(s) 46, 87, 127, 604, 760
Bme1390I CCNGG 3 cut(s) 333, 679, 855
Bme18I GGWCC 2 cut(s) 834, 857
BmeT110I CYCGRG 1 cut(s) 443
BmgT120I GGNCC 3 cut(s) 46, 834, 857
BmiI GGNNCC 3 cut(s) 48, 835, 836
BmrFI CCNGG 3 cut(s) 333, 679, 855
BmrI ACTGGG 1 cut(s) 54
BmsI GCATC 1 cut(s) 913
BmuI ACTGGG 1 cut(s) 54
BpmI CTGGAG 1 cut(s) 124
BpuEI CTTGAG 1 cut(s) 914
BsaHI GRCGYC 1 cut(s) 823
BsaJI CCNNGG 5 cut(s) 332, 444, 826, 853, 860
BsaWI WCCGGW 1 cut(s) 314
BsaXI ACNNNNNCTCC 4 cut(s) 287, 317, 782, 812
Bsc4I CCNNNNNNNGG 6 cut(s) 121, 314, 333, 338, 843, 877
Bse1I ACTGG 1 cut(s) 60
Bse3DI GCAATG 1 cut(s) 73
BseAI TCCGGA 1 cut(s) 314
BseBI CCWGG 3 cut(s) 333, 679, 855
BseDI CCNNGG 5 cut(s) 332, 444, 826, 853, 860
BseGI GGATG 1 cut(s) 343
BseLI CCNNNNNNNGG 6 cut(s) 121, 314, 333, 338, 843, 877
BseMI GCAATG 1 cut(s) 73
BseMII CTCAG 2 cut(s) 222, 886
BseNI ACTGG 1 cut(s) 60
BseXI GCAGC 3 cut(s) 112, 589, 745
BsgI GTGCAG 1 cut(s) 744
BshFI GGCC 3 cut(s) 47, 449, 962
BsiHKAI GWGCWC 1 cut(s) 237
BsiHKCI CYCGRG 1 cut(s) 443
BsiSI CCGG 1 cut(s) 315
BslFI GGGAC 2 cut(s) 834, 847
BslI CCNNNNNNNGG 6 cut(s) 121, 314, 333, 338, 843, 877
BsmAI GTCTC 3 cut(s) 142, 179, 785
BsmFI GGGAC 2 cut(s) 834, 847
BsnI GGCC 3 cut(s) 47, 449, 962
BsoBI CYCGRG 1 cut(s) 443
Bsp1286I GDGCHC 1 cut(s) 237
Bsp13I TCCGGA 1 cut(s) 314
Bsp143I GATC 1 cut(s) 719
Bsp19I CCATGG 1 cut(s) 860
BspACI CCGC 4 cut(s) 44, 86, 115, 734
BspANI GGCC 3 cut(s) 47, 449, 962
BspCNI CTCAG 2 cut(s) 223, 885
BspEI TCCGGA 1 cut(s) 314
BspLI GGNNCC 3 cut(s) 48, 835, 836
BsrDI GCAATG 1 cut(s) 73
BsrI ACTGG 1 cut(s) 60
BssECI CCNNGG 5 cut(s) 332, 444, 826, 853, 860
BssMI GATC 1 cut(s) 719
BssNI GRCGYC 1 cut(s) 823
BssT1I CCWWGG 1 cut(s) 860
Bst2UI CCWGG 3 cut(s) 333, 679, 855
Bst4CI ACNGT 5 cut(s) 136, 398, 572, 713, 796
Bst6I CTCTTC 2 cut(s) 60, 599
BstACI GRCGYC 1 cut(s) 823
BstC8I GCNNGC 1 cut(s) 756
BstDEI CTNAG 2 cut(s) 231, 872
BstDSI CCRYGG 2 cut(s) 826, 860
BstEII GGTNACC 1 cut(s) 109
BstENI CCTNNNNNAGG 1 cut(s) 875
BstF5I GGATG 1 cut(s) 343
BstHHI GCGC 1 cut(s) 130
BstKTI GATC 1 cut(s) 722
BstMAI GTCTC 3 cut(s) 142, 179, 785
BstMBI GATC 1 cut(s) 719
BstMWI GCNNNNNNNGC 1 cut(s) 254
BstNI CCWGG 3 cut(s) 333, 679, 855
BstPI GGTNACC 1 cut(s) 109
BstSCI CCNGG 3 cut(s) 331, 677, 853
BstV1I GCAGC 3 cut(s) 112, 589, 745
BstX2I RGATCY 1 cut(s) 719
BstXI CCANNNNNNTGG 1 cut(s) 861
BstYI RGATCY 1 cut(s) 719
BsuI GTATCC 1 cut(s) 884
BsuRI GGCC 3 cut(s) 47, 449, 962
BtgI CCRYGG 2 cut(s) 826, 860
BtsCI GGATG 1 cut(s) 343
BtsIMutI CAGTG 1 cut(s) 67
Cac8I GCNNGC 1 cut(s) 756
CfoI GCGC 1 cut(s) 130
Cfr13I GGNCC 3 cut(s) 46, 834, 857
CseI GACGC 1 cut(s) 831
Csp6I GTAC 2 cut(s) 329, 799
CviAII CATG 2 cut(s) 861, 919
CviQI GTAC 2 cut(s) 329, 799
DdeI CTNAG 2 cut(s) 231, 872
DpnI GATC 1 cut(s) 721
DpnII GATC 1 cut(s) 719
Eam1104I CTCTTC 2 cut(s) 60, 599
EarI CTCTTC 2 cut(s) 60, 599
EciI GGCGGA 1 cut(s) 723
Ecl136II GAGCTC 1 cut(s) 235
Eco130I CCWWGG 1 cut(s) 860
Eco24I GRGCYC 1 cut(s) 237
Eco47I GGWCC 2 cut(s) 834, 857
Eco53kI GAGCTC 1 cut(s) 235
Eco88I CYCGRG 1 cut(s) 443
Eco91I GGTNACC 1 cut(s) 109
EcoICRI GAGCTC 1 cut(s) 235
EcoNI CCTNNNNNAGG 1 cut(s) 875
EcoO109I RGGNCCY 1 cut(s) 834
EcoO65I GGTNACC 1 cut(s) 109
EcoRI GAATTC 1 cut(s) 163
EcoRII CCWGG 3 cut(s) 331, 677, 853
EcoT14I CCWWGG 1 cut(s) 860
EcoT38I GRGCYC 1 cut(s) 237
ErhI CCWWGG 1 cut(s) 860
FaeI CATG 2 cut(s) 864, 922
FaiI YATR 7 cut(s) 99, 182, 371, 716, 788, 862, 920
FaqI GGGAC 2 cut(s) 834, 847
FatI CATG 2 cut(s) 860, 918
FauI CCCGC 1 cut(s) 122
Fnu4HI GCNGC 5 cut(s) 45, 86, 126, 603, 759
FokI GGATG 1 cut(s) 350
FriOI GRGCYC 1 cut(s) 237
Fsp4HI GCNGC 5 cut(s) 45, 86, 126, 603, 759
FspBI CTAG 2 cut(s) 219, 420
FspI TGCGCA 1 cut(s) 129
GlaI GCGC 1 cut(s) 129
GluI GCNGC 5 cut(s) 45, 86, 126, 603, 759
GsuI CTGGAG 1 cut(s) 124
HaeIII GGCC 3 cut(s) 47, 449, 962
HapII CCGG 1 cut(s) 315
HgaI GACGC 1 cut(s) 831
HhaI GCGC 1 cut(s) 130
Hin1I GRCGYC 1 cut(s) 823
Hin1II CATG 2 cut(s) 864, 922
Hin6I GCGC 1 cut(s) 128
HinP1I GCGC 1 cut(s) 128
HinfI GANTC 2 cut(s) 323, 473
HpaII CCGG 1 cut(s) 315
HphI GGTGA 3 cut(s) 103, 353, 616
Hpy166II GTNNAC 1 cut(s) 799
Hpy188I TCNGA 5 cut(s) 13, 232, 513, 669, 875
Hpy188III TCNNGA 7 cut(s) 141, 157, 315, 383, 410, 443, 723
Hpy8I GTNNAC 1 cut(s) 799
Hpy99I CGWCG 1 cut(s) 928
HpyAV CCTTC 4 cut(s) 60, 268, 338, 748
HpyCH4III ACNGT 5 cut(s) 136, 398, 572, 713, 796
HpyCH4IV ACGT 2 cut(s) 311, 894
HpyCH4V TGCA 6 cut(s) 257, 373, 393, 467, 761, 904
HpyF10VI GCNNNNNNNGC 1 cut(s) 254
HpyF3I CTNAG 2 cut(s) 231, 872
HpySE526I ACGT 2 cut(s) 311, 894
Hsp92I GRCGYC 1 cut(s) 823
Hsp92II CATG 2 cut(s) 864, 922
HspAI GCGC 1 cut(s) 128
KflI GGGWCCC 1 cut(s) 834
Kpn2I TCCGGA 1 cut(s) 314
Kzo9I GATC 1 cut(s) 719
LmnI GCTCC 1 cut(s) 846
Lsp1109I GCAGC 3 cut(s) 112, 589, 745
LweI GCATC 1 cut(s) 913
MaeI CTAG 2 cut(s) 219, 420
MaeII ACGT 2 cut(s) 311, 894
MaeIII GTNAC 3 cut(s) 19, 109, 622
MalI GATC 1 cut(s) 721
MboI GATC 1 cut(s) 719
MboII GAAGA 6 cut(s) 47, 586, 596, 656, 733, 944
MflI RGATCY 1 cut(s) 719
MhlI GDGCHC 1 cut(s) 237
MluCI AATT 8 cut(s) 103, 152, 163, 263, 378, 386, 461, 811
MmeI TCCRAC 1 cut(s) 483
MnlI CCTC 8 cut(s) 37, 153, 158, 341, 439, 526, 824, 881
MroI TCCGGA 1 cut(s) 314
MroXI GAANNNNTTC 1 cut(s) 663
MseI TTAA 6 cut(s) 377, 401, 459, 498, 618, 816
MslI CAYNNNNRTG 1 cut(s) 629
MspI CCGG 1 cut(s) 315
MspR9I CCNGG 3 cut(s) 333, 679, 855
MvaI CCWGG 3 cut(s) 333, 679, 855
MwoI GCNNNNNNNGC 1 cut(s) 254
NcoI CCATGG 1 cut(s) 860
NdeII GATC 1 cut(s) 719
NlaIII CATG 2 cut(s) 864, 922
NlaIV GGNNCC 3 cut(s) 48, 835, 836
NmuCI GTSAC 2 cut(s) 109, 622
NsbI TGCGCA 1 cut(s) 129
PdmI GAANNNNTTC 1 cut(s) 663
PfeI GAWTC 2 cut(s) 323, 473
PflMI CCANNNNNTGG 1 cut(s) 338
PfoI TCCNGGA 1 cut(s) 677
PkrI GCNGC 5 cut(s) 46, 87, 127, 604, 760
PpuMI RGGWCCY 1 cut(s) 834
Psp124BI GAGCTC 1 cut(s) 237
Psp5II RGGWCCY 1 cut(s) 834
Psp6I CCWGG 3 cut(s) 331, 677, 853
PspEI GGTNACC 1 cut(s) 109
PspGI CCWGG 3 cut(s) 331, 677, 853
PspN4I GGNNCC 3 cut(s) 48, 835, 836
PspPI GGNCC 3 cut(s) 46, 834, 857
PspPPI RGGWCCY 1 cut(s) 834
PsuI RGATCY 1 cut(s) 719
RsaI GTAC 2 cut(s) 330, 800
RsaNI GTAC 2 cut(s) 329, 799
RseI CAYNNNNRTG 1 cut(s) 629
SacI GAGCTC 1 cut(s) 237
SaqAI TTAA 6 cut(s) 377, 401, 459, 498, 618, 816
SatI GCNGC 5 cut(s) 45, 86, 126, 603, 759
Sau3AI GATC 1 cut(s) 719
Sau96I GGNCC 3 cut(s) 46, 834, 857
ScrFI CCNGG 3 cut(s) 333, 679, 855
SduI GDGCHC 1 cut(s) 237
SfaNI GCATC 1 cut(s) 913
SinI GGWCC 2 cut(s) 834, 857
SmiMI CAYNNNNRTG 1 cut(s) 629
SmlI CTYRAG 1 cut(s) 929
SmoI CTYRAG 1 cut(s) 929
Sse9I AATT 8 cut(s) 103, 152, 163, 263, 378, 386, 461, 811
SsiI CCGC 4 cut(s) 44, 86, 115, 734
SspI AATATT 2 cut(s) 435, 480
SspMI CTAG 2 cut(s) 219, 420
SstI GAGCTC 1 cut(s) 237
StyD4I CCNGG 3 cut(s) 331, 677, 853
StyI CCWWGG 1 cut(s) 860
TaaI ACNGT 5 cut(s) 136, 398, 572, 713, 796
TaiI ACGT 2 cut(s) 314, 897
TaqI TCGA 2 cut(s) 382, 642
TasI AATT 8 cut(s) 103, 152, 163, 263, 378, 386, 461, 811
TatI WGTACW 1 cut(s) 798
TauI GCSGC 2 cut(s) 47, 88
TfiI GAWTC 2 cut(s) 323, 473
Tru1I TTAA 6 cut(s) 377, 401, 459, 498, 618, 816
Tru9I TTAA 6 cut(s) 377, 401, 459, 498, 618, 816
TscAI CASTG 1 cut(s) 67
TseFI GTSAC 2 cut(s) 109, 622
TseI GCWGC 3 cut(s) 125, 602, 758
Tsp45I GTSAC 2 cut(s) 109, 622
TspDTI ATGAA 1 cut(s) 603
TspGWI ACGGA 5 cut(s) 27, 316, 600, 843, 903
TspRI CASTG 1 cut(s) 67
Van91I CCANNNNNTGG 1 cut(s) 338
VpaK11BI GGWCC 2 cut(s) 834, 857
XagI CCTNNNNNAGG 1 cut(s) 875
XapI RAATTY 5 cut(s) 103, 152, 163, 386, 461
XmnI GAANNNNTTC 1 cut(s) 663
XspI CTAG 2 cut(s) 219, 420
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.