pycom16g25400

Domain of unknown function (DUF1995)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0000465
Physical Location & Seq
Forward (+)
27235760 .. 27238339
2580 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g25400.3

Sequence Viewer

Length: 960 bp
ATGCCGTCGTCCGACATCGTAACTGCTTCTAAGGTTCCGTTTGCGGCCCCTTCTCTTCCCAATGGCAAGAAGCATTTGCCGCCAAAATCCTATGGGAATTTGGTCACCCGCAAAATGGCTGCGCAAACTGTCTCCAGAAACCTCAAATTCGTGAGGAATTCGGCTTCGGGGGATATGGAGACAAGTTCAGTTGGGCTTGATGTGTCAATTCCTAGCGACTATTCTGAGCTCATTGAACAAGCCCGAAATGCAACTGCATTGGCTGTGAAGGATAACAAGCAGTTGATGGAGATTGAGTTCCCTACTTCCGGACTTGAATCCGTACCAGGGGATGGTGAAGGGGGAATAGAAATGACAGAAAGTATGCAGTTAATTCGAGAATTTTGCAACCGTTTAATAGTTCCAGAGAAAGCCAGACAGACCAGAATATTTTTTCCTGAGGCCAACGAAGTTAAATTTGCAAGAGAATCAATATTTGAGGGTGCTTCCTTTAAGTTGGACTATCTGACAAAGCCATCATTCTTTGAGGACTTTGGTTTTGGGGAGAAAGTGAAAATGACAGACCGTGTGAAGGCAGAAGATGAACTCTTCCTTGCTGCCTATCCATATTTTAATGTCACTGAAATGCTTGTGGTCGAAGAACTTTACAGAGAAGCTGTTCAGAACACTTCAAGGAAACTGATTATTTTCAATGGGGAGCTTGACCGTATAAGATCTGGATATTATCCGCCTTTCTTCTACCCAAAGCTCGCTGCACTTTCCAAGAGCCTATTTCCATTTATGGAGACTGTGTACTACATTCACAACTTTAAGGGACGCTACGGAGGGACCCTTTTCAGGAGCTACCCAGGTCCATGGAAAGTCTTCAGAAAAGTAAGGAACGGATACGTCTGTTTGCATCAGCAGGAAGCAATGCCGTCGCTCAAGGAAGTTGCTTTAGATATTCTTCCATCGGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1995

Protein Analysis

320

Amino Acids

36.07

Weight (kDa)

7.67

Isoelectric Point (pI)

43.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000465)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48790 AT5G48790 AT5G48790
fragaria_vesca FvH4_5g23760 FvH4_5g23760 FvH4_5g23770 FvH4_5g23771 FvH4_5g23790 FvH4_5g23800 FvH4_7g03421 FvH4_7g07510
malus_domestica MD00G1000600.v1.1 MD06G1013900.v1.1
prunus_persica Prupe.2G101800_v2.0.a1 Prupe.5G022600_v2.0.a1 Prupe.5G022700_v2.0.a1
pyrus_communis pycom07g06280 pycom16g25400
rosa_chinensis RchiOBHm_Chr1g0337881 RchiOBHm_Chr1g0339341 RchiOBHm_Chr7g0213081 RchiOBHm_Chr7g0213111 RchiOBHm_Chr7g0213141 RchiOBHm_Chr7g0213151 RchiOBHm_Chr7g0213161 RchiOBHm_Chr7g0213181
rosa_laevigata RLG00000002825 RLG00000002828 RLG00000002829 RLG00000002832 RLG00000029226
rosa_multiflora Rmu_co8345885.1_g000002 Rmu_co8348513.1_g000001 Rmu_co8456259.1_g000001 Rmu_sc0000613.1_g000002 Rmu_sc0001121.1_g000005 Rmu_sc0001121.1_g000006 Rmu_sc0002989.1_g000003 Rmu_sc0015110.1_g000001 Rmu_sc0030208.1_g000001 Rmu_sc0030209.1_g000001 Rmu_sc0032877.1_g000001 Rmu_sc0039394.1_g000001 Rmu_ssc0000084.1_g000002 Rmu_ssc0000084.1_g000007 Rmu_ssc0000084.1_g000009 Rmu_ssc0000084.1_g000012
rosa_roxburghii Rroxscaffold_3G00246200 Rroxscaffold_3G00246210 Rroxscaffold_3G00246240 Rroxscaffold_3G00246250 Rroxscaffold_4G00313440
rosa_rugosa Rorug01G0142600.1 Rorug07G0139400 Rorug07G0139500 Rorug07G0139600 Rorug07G0139700.1 Rorug07G0139800 Rorug07G0140100
rosa_samantha Rh1AG159300 Rh1BG126400 Rh1CG148500 Rh7AG270700 Rh7AG270800 Rh7AG271200 Rh7AG271400 Rh7AG271500 Rh7BG266400 Rh7BG266600 Rh7BG267000 Rh7BG267100 Rh7BG267200 Rh7BG267400 Rh7CG290000 Rh7CG290100 Rh7CG290600 Rh7CG290700 Rh7CG290800 Rh7DG278900 Rh7DG279200 Rh7DG279300 Rh7DG279400 Rh7DG279500 Rh7DG279600
rosa_wichuraiana Rw1G013550 Rw7G023520 Rw7G023530 Rw7G023540 Rw7G023550 Rw7G023560 Rw7G023580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 123
AccB7I CCANNNNNTGG 1 cut(s) 332
AccIII TCCGGA 1 cut(s) 308
AciI CCGC 4 cut(s) 44, 80, 109, 728
AcsI RAATTY 5 cut(s) 97, 146, 157, 380, 455
AcuI CTGAAG 1 cut(s) 850
AfaI GTAC 2 cut(s) 324, 794
AfiI CCNNNNNNNGG 6 cut(s) 115, 308, 327, 332, 571, 837
AgsI TTSAA 4 cut(s) 236, 317, 672, 691
AjnI CCWGG 2 cut(s) 325, 847
AloI GAACNNNNNNTCC 2 cut(s) 281, 313
AluBI AGCT 5 cut(s) 229, 656, 700, 748, 843
AluI AGCT 5 cut(s) 229, 656, 700, 748, 843
Alw21I GWGCWC 1 cut(s) 231
Alw26I GTCTC 3 cut(s) 136, 173, 779
Aor13HI TCCGGA 1 cut(s) 308
AoxI GGCC 3 cut(s) 45, 441, 954
ApeKI GCWGC 3 cut(s) 119, 596, 752
ApoI RAATTY 5 cut(s) 97, 146, 157, 380, 455
ArsI GACNNNNNNTTYG 2 cut(s) 521, 553
Asp700I GAANNNNTTC 2 cut(s) 657, 863
AspLEI GCGC 1 cut(s) 124
AspS9I GGNCC 3 cut(s) 46, 828, 851
AsuHPI GGTGA 2 cut(s) 97, 347
AvaII GGWCC 2 cut(s) 828, 851
AxyI CCTNAGG 1 cut(s) 438
BaeI ACNNNNGTAYC 2 cut(s) 877, 910
BanII GRGCYC 1 cut(s) 231
BbsI GAAGAC 1 cut(s) 856
Bbv12I GWGCWC 1 cut(s) 231
BbvI GCAGC 3 cut(s) 106, 583, 739
BccI CCATC 4 cut(s) 280, 326, 523, 958
BceAI ACGGC 1 cut(s) 901
BcgI CGANNNNNNTGC 6 cut(s) 356, 366, 390, 400, 900, 934
BciT130I CCWGG 2 cut(s) 327, 849
BciVI GTATCC 1 cut(s) 878
BcoDI GTCTC 3 cut(s) 136, 173, 779
BfaI CTAG 1 cut(s) 213
BfuI GTATCC 1 cut(s) 878
BglII AGATCT 1 cut(s) 713
BisI GCNGC 5 cut(s) 45, 80, 120, 597, 753
BlsI GCNGC 5 cut(s) 46, 81, 121, 598, 754
Bme1390I CCNGG 2 cut(s) 327, 849
Bme18I GGWCC 2 cut(s) 828, 851
BmgT120I GGNCC 3 cut(s) 46, 828, 851
BmiI GGNNCC 4 cut(s) 36, 48, 829, 830
BmrFI CCNGG 2 cut(s) 327, 849
BmsI GCATC 1 cut(s) 907
BpiI GAAGAC 1 cut(s) 856
BpmI CTGGAG 1 cut(s) 118
BpuEI CTTGAG 1 cut(s) 908
BsaJI CCNNGG 3 cut(s) 326, 847, 854
BsaWI WCCGGW 1 cut(s) 308
BsaXI ACNNNNNCTCC 4 cut(s) 281, 311, 776, 806
Bsc4I CCNNNNNNNGG 6 cut(s) 115, 308, 327, 332, 571, 837
Bse21I CCTNAGG 1 cut(s) 438
Bse3DI GCAATG 1 cut(s) 918
BseAI TCCGGA 1 cut(s) 308
BseBI CCWGG 2 cut(s) 327, 849
BseDI CCNNGG 3 cut(s) 326, 847, 854
BseGI GGATG 1 cut(s) 337
BseLI CCNNNNNNNGG 6 cut(s) 115, 308, 327, 332, 571, 837
BseMI GCAATG 1 cut(s) 918
BseMII CTCAG 2 cut(s) 216, 429
BseXI GCAGC 3 cut(s) 106, 583, 739
BsgI GTGCAG 1 cut(s) 738
BshFI GGCC 3 cut(s) 47, 443, 956
BsiHKAI GWGCWC 1 cut(s) 231
BsiSI CCGG 1 cut(s) 309
BslFI GGGAC 2 cut(s) 828, 841
BslI CCNNNNNNNGG 6 cut(s) 115, 308, 327, 332, 571, 837
BsmAI GTCTC 3 cut(s) 136, 173, 779
BsmFI GGGAC 2 cut(s) 828, 841
BsnI GGCC 3 cut(s) 47, 443, 956
Bsp1286I GDGCHC 1 cut(s) 231
Bsp13I TCCGGA 1 cut(s) 308
Bsp143I GATC 1 cut(s) 713
Bsp19I CCATGG 1 cut(s) 854
BspACI CCGC 4 cut(s) 44, 80, 109, 728
BspANI GGCC 3 cut(s) 47, 443, 956
BspCNI CTCAG 2 cut(s) 217, 430
BspEI TCCGGA 1 cut(s) 308
BspLI GGNNCC 4 cut(s) 36, 48, 829, 830
BsrDI GCAATG 1 cut(s) 918
BssECI CCNNGG 3 cut(s) 326, 847, 854
BssMI GATC 1 cut(s) 713
BssT1I CCWWGG 1 cut(s) 854
Bst2UI CCWGG 2 cut(s) 327, 849
Bst4CI ACNGT 5 cut(s) 130, 392, 566, 707, 790
Bst6I CTCTTC 2 cut(s) 60, 593
BstC8I GCNNGC 1 cut(s) 750
BstDEI CTNAG 3 cut(s) 30, 225, 438
BstDSI CCRYGG 1 cut(s) 854
BstEII GGTNACC 1 cut(s) 103
BstF5I GGATG 1 cut(s) 337
BstHHI GCGC 1 cut(s) 124
BstKTI GATC 1 cut(s) 716
BstMAI GTCTC 3 cut(s) 136, 173, 779
BstMBI GATC 1 cut(s) 713
BstMWI GCNNNNNNNGC 2 cut(s) 79, 248
BstNI CCWGG 2 cut(s) 327, 849
BstPI GGTNACC 1 cut(s) 103
BstSCI CCNGG 2 cut(s) 325, 847
BstV1I GCAGC 3 cut(s) 106, 583, 739
BstV2I GAAGAC 1 cut(s) 856
BstX2I RGATCY 1 cut(s) 713
BstXI CCANNNNNNTGG 1 cut(s) 855
BstYI RGATCY 1 cut(s) 713
Bsu36I CCTNAGG 1 cut(s) 438
BsuI GTATCC 1 cut(s) 878
BsuRI GGCC 3 cut(s) 47, 443, 956
BtgI CCRYGG 1 cut(s) 854
BtsCI GGATG 1 cut(s) 337
BtsIMutI CAGTG 1 cut(s) 618
Cac8I GCNNGC 1 cut(s) 750
CfoI GCGC 1 cut(s) 124
Cfr13I GGNCC 3 cut(s) 46, 828, 851
CseI GACGC 1 cut(s) 825
Csp6I GTAC 2 cut(s) 323, 793
CviAII CATG 1 cut(s) 855
CviQI GTAC 2 cut(s) 323, 793
DdeI CTNAG 3 cut(s) 30, 225, 438
DpnI GATC 1 cut(s) 715
DpnII GATC 1 cut(s) 713
Eam1104I CTCTTC 2 cut(s) 60, 593
EarI CTCTTC 2 cut(s) 60, 593
EciI GGCGGA 1 cut(s) 717
Ecl136II GAGCTC 1 cut(s) 229
Eco130I CCWWGG 1 cut(s) 854
Eco24I GRGCYC 1 cut(s) 231
Eco47I GGWCC 2 cut(s) 828, 851
Eco53kI GAGCTC 1 cut(s) 229
Eco57I CTGAAG 1 cut(s) 850
Eco81I CCTNAGG 1 cut(s) 438
Eco91I GGTNACC 1 cut(s) 103
EcoICRI GAGCTC 1 cut(s) 229
EcoO109I RGGNCCY 1 cut(s) 828
EcoO65I GGTNACC 1 cut(s) 103
EcoRI GAATTC 1 cut(s) 157
EcoRII CCWGG 2 cut(s) 325, 847
EcoT14I CCWWGG 1 cut(s) 854
EcoT38I GRGCYC 1 cut(s) 231
ErhI CCWWGG 1 cut(s) 854
FaeI CATG 1 cut(s) 858
FaiI YATR 7 cut(s) 93, 176, 365, 607, 710, 782, 856
FaqI GGGAC 2 cut(s) 828, 841
FatI CATG 1 cut(s) 854
FauI CCCGC 1 cut(s) 116
Fnu4HI GCNGC 5 cut(s) 45, 80, 120, 597, 753
FokI GGATG 1 cut(s) 344
FriOI GRGCYC 1 cut(s) 231
Fsp4HI GCNGC 5 cut(s) 45, 80, 120, 597, 753
FspBI CTAG 1 cut(s) 213
FspI TGCGCA 1 cut(s) 123
GlaI GCGC 1 cut(s) 123
GluI GCNGC 5 cut(s) 45, 80, 120, 597, 753
GsuI CTGGAG 1 cut(s) 118
HaeIII GGCC 3 cut(s) 47, 443, 956
HapII CCGG 1 cut(s) 309
HgaI GACGC 1 cut(s) 825
HhaI GCGC 1 cut(s) 124
Hin1II CATG 1 cut(s) 858
Hin6I GCGC 1 cut(s) 122
HinP1I GCGC 1 cut(s) 122
HinfI GANTC 2 cut(s) 317, 467
HpaII CCGG 1 cut(s) 309
HphI GGTGA 2 cut(s) 97, 347
Hpy166II GTNNAC 1 cut(s) 793
Hpy188I TCNGA 5 cut(s) 13, 226, 507, 663, 869
Hpy188III TCNNGA 8 cut(s) 135, 151, 309, 377, 404, 437, 717, 838
Hpy8I GTNNAC 1 cut(s) 793
Hpy99I CGWCG 2 cut(s) 10, 922
HpyAV CCTTC 4 cut(s) 60, 262, 332, 565
HpyCH4III ACNGT 5 cut(s) 130, 392, 566, 707, 790
HpyCH4IV ACGT 1 cut(s) 888
HpyCH4V TGCA 7 cut(s) 251, 257, 367, 387, 461, 755, 898
HpyF10VI GCNNNNNNNGC 2 cut(s) 79, 248
HpyF3I CTNAG 3 cut(s) 30, 225, 438
HpySE526I ACGT 1 cut(s) 888
Hsp92II CATG 1 cut(s) 858
HspAI GCGC 1 cut(s) 122
KflI GGGWCCC 1 cut(s) 828
Kpn2I TCCGGA 1 cut(s) 308
Kzo9I GATC 1 cut(s) 713
LmnI GCTCC 2 cut(s) 697, 840
Lsp1109I GCAGC 3 cut(s) 106, 583, 739
LweI GCATC 1 cut(s) 907
MaeI CTAG 1 cut(s) 213
MaeII ACGT 1 cut(s) 888
MaeIII GTNAC 3 cut(s) 19, 103, 616
MalI GATC 1 cut(s) 715
MboI GATC 1 cut(s) 713
MboII GAAGA 7 cut(s) 47, 580, 590, 650, 727, 856, 938
MflI RGATCY 1 cut(s) 713
MhlI GDGCHC 1 cut(s) 231
MluCI AATT 7 cut(s) 97, 146, 157, 207, 372, 380, 455
MmeI TCCRAC 2 cut(s) 36, 477
MnlI CCTC 6 cut(s) 147, 152, 433, 472, 520, 818
MroI TCCGGA 1 cut(s) 308
MroXI GAANNNNTTC 2 cut(s) 657, 863
MseI TTAA 6 cut(s) 371, 395, 453, 492, 612, 810
MslI CAYNNNNRTG 1 cut(s) 623
MspI CCGG 1 cut(s) 309
MspR9I CCNGG 2 cut(s) 327, 849
MvaI CCWGG 2 cut(s) 327, 849
MwoI GCNNNNNNNGC 2 cut(s) 79, 248
NcoI CCATGG 1 cut(s) 854
NdeII GATC 1 cut(s) 713
NlaIII CATG 1 cut(s) 858
NlaIV GGNNCC 4 cut(s) 36, 48, 829, 830
NmuCI GTSAC 2 cut(s) 103, 616
NsbI TGCGCA 1 cut(s) 123
PdmI GAANNNNTTC 2 cut(s) 657, 863
PfeI GAWTC 2 cut(s) 317, 467
PflMI CCANNNNNTGG 1 cut(s) 332
PkrI GCNGC 5 cut(s) 46, 81, 121, 598, 754
PpuMI RGGWCCY 1 cut(s) 828
Psp124BI GAGCTC 1 cut(s) 231
Psp5II RGGWCCY 1 cut(s) 828
Psp6I CCWGG 2 cut(s) 325, 847
PspEI GGTNACC 1 cut(s) 103
PspGI CCWGG 2 cut(s) 325, 847
PspN4I GGNNCC 4 cut(s) 36, 48, 829, 830
PspPI GGNCC 3 cut(s) 46, 828, 851
PspPPI RGGWCCY 1 cut(s) 828
PsuI RGATCY 1 cut(s) 713
RsaI GTAC 2 cut(s) 324, 794
RsaNI GTAC 2 cut(s) 323, 793
RseI CAYNNNNRTG 1 cut(s) 623
SacI GAGCTC 1 cut(s) 231
SaqAI TTAA 6 cut(s) 371, 395, 453, 492, 612, 810
SatI GCNGC 5 cut(s) 45, 80, 120, 597, 753
Sau3AI GATC 1 cut(s) 713
Sau96I GGNCC 3 cut(s) 46, 828, 851
ScrFI CCNGG 2 cut(s) 327, 849
SduI GDGCHC 1 cut(s) 231
SetI ASST 9 cut(s) 36, 144, 231, 658, 702, 750, 845, 853, 891
SfaNI GCATC 1 cut(s) 907
SinI GGWCC 2 cut(s) 828, 851
SmiMI CAYNNNNRTG 1 cut(s) 623
SmlI CTYRAG 1 cut(s) 923
SmoI CTYRAG 1 cut(s) 923
Sse9I AATT 7 cut(s) 97, 146, 157, 207, 372, 380, 455
SsiI CCGC 4 cut(s) 44, 80, 109, 728
SspI AATATT 2 cut(s) 429, 474
SspMI CTAG 1 cut(s) 213
SstI GAGCTC 1 cut(s) 231
StyD4I CCNGG 2 cut(s) 325, 847
StyI CCWWGG 1 cut(s) 854
TaaI ACNGT 5 cut(s) 130, 392, 566, 707, 790
TaiI ACGT 1 cut(s) 891
TaqI TCGA 2 cut(s) 376, 636
TasI AATT 7 cut(s) 97, 146, 157, 207, 372, 380, 455
TatI WGTACW 1 cut(s) 792
TauI GCSGC 2 cut(s) 47, 82
TfiI GAWTC 2 cut(s) 317, 467
Tru1I TTAA 6 cut(s) 371, 395, 453, 492, 612, 810
Tru9I TTAA 6 cut(s) 371, 395, 453, 492, 612, 810
TscAI CASTG 1 cut(s) 625
TseFI GTSAC 2 cut(s) 103, 616
TseI GCWGC 3 cut(s) 119, 596, 752
Tsp45I GTSAC 2 cut(s) 103, 616
TspDTI ATGAA 1 cut(s) 597
TspGWI ACGGA 4 cut(s) 27, 310, 837, 897
TspRI CASTG 1 cut(s) 625
Van91I CCANNNNNTGG 1 cut(s) 332
VpaK11BI GGWCC 2 cut(s) 828, 851
XapI RAATTY 5 cut(s) 97, 146, 157, 380, 455
XmnI GAANNNNTTC 2 cut(s) 657, 863
XspI CTAG 1 cut(s) 213
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.