Prupe.5G022700_v2.0.a1

Domain of unknown function (DUF1995)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0000465
Physical Location & Seq
Reverse (-)
2517101 .. 2521725
4625 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G022700.1

Sequence Viewer

Length: 978 bp
ATGCCTTTATCTGAGGTTCCGTTTTCGGTGGTGCCTTCTCTTCCAAGTCGCAAGGTTGCTGCTGTTACTTGCAATTGTTTGCCACCAAAAACTTATGGCGGCAACTTTAGTACACGCAAAGTTGCTGCTGCATTCAGTGTCTCTAGAAATCTCAAATTTTTGGCAAATTCGGCTTCTCGGGATGACAACGACGCTACTGCTTCGGTTGAGTTTGATGACGTCTCAATTCCTACCGACTATTCTGAGCTGCTTGAACAAGCCAGAAAAGCAACTGATTTGGCTTTGAAGGACAACAAGCAGTTGATGGAGATTGAGTTCCCTACTTCTGGACTTGAATCTGTACCAGGGGATGGCGAAGGAGGAATAGAAATGACAGAAAGTATGCAGCTAATTCGAGAATTTTGTAACCGCTTAATAAACCCAGAGAAAGCCACCCGGACCAGAATATTCTTCCCTGAGGCCAGCGAAGTTAAATTTGCAAGAGAATCAGTTTTTGAAGGAGCTTCCTTCAAGCTGGACTATCTGACGAAGCCATCATTTTTTGAGGATTTTGGTTTTGTTGAGAAAGTGAAAATGAAAGACCGTGTGAAGCCAGAAGATGAACTTTTCCTGGCTGCCTATCCATATTTTAATGTCAATGAAATGCTCGTGGTTGAAGAACTTTACAAAGAAGCTGTTGAGAACACTTCCAGGAACCTAATTATTTTCAATGGAGAACTTGACCGCATAAGATCTGGGTATTATCCGCCTTTCTTCTACCCGAAACTCGCTGCACTTTCCCAGACCCTATTTCCAGTTATGGAGACTGTATACTACATTCACAATTTTAAGGGACGCAATGGAGGGACCCTTTTCAGGTGCTACCCAGGTCCATGGAAAGTGTTCAGAAAAGTGAGGAACAGATACGTTTGTTTGCACCAGCAGGAAGCTATGCCGTCGCTCAAGGAAGTGGCTTTAGATATTCTTCCATCGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1995

Protein Analysis

326

Amino Acids

36.88

Weight (kDa)

5.55

Isoelectric Point (pI)

42.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000465)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48790 AT5G48790 AT5G48790
fragaria_vesca FvH4_5g23760 FvH4_5g23760 FvH4_5g23770 FvH4_5g23771 FvH4_5g23790 FvH4_5g23800 FvH4_7g03421 FvH4_7g07510
malus_domestica MD00G1000600.v1.1 MD06G1013900.v1.1
prunus_persica Prupe.2G101800_v2.0.a1 Prupe.5G022600_v2.0.a1 Prupe.5G022700_v2.0.a1
pyrus_communis pycom07g06280 pycom16g25400
rosa_chinensis RchiOBHm_Chr1g0337881 RchiOBHm_Chr1g0339341 RchiOBHm_Chr7g0213081 RchiOBHm_Chr7g0213111 RchiOBHm_Chr7g0213141 RchiOBHm_Chr7g0213151 RchiOBHm_Chr7g0213161 RchiOBHm_Chr7g0213181
rosa_laevigata RLG00000002825 RLG00000002828 RLG00000002829 RLG00000002832 RLG00000029226
rosa_multiflora Rmu_co8345885.1_g000002 Rmu_co8348513.1_g000001 Rmu_co8456259.1_g000001 Rmu_sc0000613.1_g000002 Rmu_sc0001121.1_g000005 Rmu_sc0001121.1_g000006 Rmu_sc0002989.1_g000003 Rmu_sc0015110.1_g000001 Rmu_sc0030208.1_g000001 Rmu_sc0030209.1_g000001 Rmu_sc0032877.1_g000001 Rmu_sc0039394.1_g000001 Rmu_ssc0000084.1_g000002 Rmu_ssc0000084.1_g000007 Rmu_ssc0000084.1_g000009 Rmu_ssc0000084.1_g000012
rosa_roxburghii Rroxscaffold_3G00246200 Rroxscaffold_3G00246210 Rroxscaffold_3G00246240 Rroxscaffold_3G00246250 Rroxscaffold_4G00313440
rosa_rugosa Rorug01G0142600.1 Rorug07G0139400 Rorug07G0139500 Rorug07G0139600 Rorug07G0139700.1 Rorug07G0139800 Rorug07G0140100
rosa_samantha Rh1AG159300 Rh1BG126400 Rh1CG148500 Rh7AG270700 Rh7AG270800 Rh7AG271200 Rh7AG271400 Rh7AG271500 Rh7BG266400 Rh7BG266600 Rh7BG267000 Rh7BG267100 Rh7BG267200 Rh7BG267400 Rh7CG290000 Rh7CG290100 Rh7CG290600 Rh7CG290700 Rh7CG290800 Rh7DG278900 Rh7DG279200 Rh7DG279300 Rh7DG279400 Rh7DG279500 Rh7DG279600
rosa_wichuraiana Rw1G013550 Rw7G023520 Rw7G023530 Rw7G023540 Rw7G023550 Rw7G023560 Rw7G023580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 222
AccB1I GGYRCC 1 cut(s) 31
AccB7I CCANNNNNTGG 1 cut(s) 350
AccI GTMKAC 1 cut(s) 810
AciI CCGC 4 cut(s) 99, 409, 724, 746
AcsI RAATTY 4 cut(s) 155, 166, 398, 473
AcyI GRCGYC 1 cut(s) 219
AfaI GTAC 2 cut(s) 112, 342
AfiI CCNNNNNNNGG 3 cut(s) 326, 350, 855
AgsI TTSAA 7 cut(s) 254, 286, 335, 497, 511, 656, 709
AjnI CCWGG 4 cut(s) 343, 609, 689, 865
AloI GAACNNNNNNTCC 2 cut(s) 299, 331
AluBI AGCT 6 cut(s) 247, 388, 503, 514, 674, 929
AluI AGCT 6 cut(s) 247, 388, 503, 514, 674, 929
Alw26I GTCTC 3 cut(s) 145, 226, 797
Ama87I CYCGRG 1 cut(s) 177
AoxI GGCC 1 cut(s) 459
ApeKI GCWGC 7 cut(s) 59, 125, 128, 247, 385, 614, 770
ApoI RAATTY 4 cut(s) 155, 166, 398, 473
Asp700I GAANNNNTTC 1 cut(s) 881
AspS9I GGNCC 3 cut(s) 438, 846, 869
AsuC2I CCSGG 1 cut(s) 436
AvaI CYCGRG 1 cut(s) 177
AvaII GGWCC 3 cut(s) 438, 846, 869
AxyI CCTNAGG 1 cut(s) 456
BaeI ACNNNNGTAYC 2 cut(s) 895, 928
BanI GGYRCC 1 cut(s) 31
BauI CACGAG 1 cut(s) 647
BbvI GCAGC 7 cut(s) 46, 112, 115, 234, 397, 601, 757
BccI CCATC 4 cut(s) 298, 344, 541, 976
BceAI ACGGC 1 cut(s) 919
BcgI CGANNNNNNTGC 4 cut(s) 179, 213, 374, 408
BciT130I CCWGG 4 cut(s) 345, 611, 691, 867
BcnI CCSGG 1 cut(s) 436
BcoDI GTCTC 3 cut(s) 145, 226, 797
BfaI CTAG 1 cut(s) 144
BglII AGATCT 1 cut(s) 731
BisI GCNGC 8 cut(s) 60, 100, 126, 129, 248, 386, 615, 771
BlsI GCNGC 8 cut(s) 61, 101, 127, 130, 249, 387, 616, 772
Bme1390I CCNGG 5 cut(s) 345, 436, 611, 691, 867
Bme18I GGWCC 3 cut(s) 438, 846, 869
BmeT110I CYCGRG 1 cut(s) 177
BmgT120I GGNCC 3 cut(s) 438, 846, 869
BmiI GGNNCC 5 cut(s) 18, 33, 695, 847, 848
BmrFI CCNGG 5 cut(s) 345, 436, 611, 691, 867
BpuEI CTTGAG 1 cut(s) 926
BpuMI CCSGG 1 cut(s) 436
BsaHI GRCGYC 1 cut(s) 219
BsaJI CCNNGG 3 cut(s) 344, 865, 872
BsaXI ACNNNNNCTCC 2 cut(s) 299, 329
Bsc4I CCNNNNNNNGG 3 cut(s) 326, 350, 855
Bse1I ACTGG 1 cut(s) 794
Bse21I CCTNAGG 1 cut(s) 456
Bse3DI GCAATG 1 cut(s) 844
BseBI CCWGG 4 cut(s) 345, 611, 691, 867
BseDI CCNNGG 3 cut(s) 344, 865, 872
BseGI GGATG 2 cut(s) 187, 355
BseLI CCNNNNNNNGG 3 cut(s) 326, 350, 855
BseMI GCAATG 1 cut(s) 844
BseMII CTCAG 2 cut(s) 234, 447
BseNI ACTGG 1 cut(s) 794
BseXI GCAGC 7 cut(s) 46, 112, 115, 234, 397, 601, 757
BsgI GTGCAG 1 cut(s) 756
BshFI GGCC 1 cut(s) 461
BshNI GGYRCC 1 cut(s) 31
BsiHKCI CYCGRG 1 cut(s) 177
BsiSI CCGG 1 cut(s) 436
BslFI GGGAC 2 cut(s) 846, 859
BslI CCNNNNNNNGG 3 cut(s) 326, 350, 855
BsmAI GTCTC 3 cut(s) 145, 226, 797
BsmBI CGTCTC 1 cut(s) 226
BsmFI GGGAC 2 cut(s) 846, 859
BsmI GAATGC 1 cut(s) 131
BsnI GGCC 1 cut(s) 461
BsoBI CYCGRG 1 cut(s) 177
Bsp143I GATC 1 cut(s) 731
Bsp19I CCATGG 1 cut(s) 872
BspACI CCGC 4 cut(s) 99, 409, 724, 746
BspANI GGCC 1 cut(s) 461
BspCNI CTCAG 3 cut(s) 4, 235, 448
BspLI GGNNCC 5 cut(s) 18, 33, 695, 847, 848
BspT107I GGYRCC 1 cut(s) 31
BsrDI GCAATG 1 cut(s) 844
BsrI ACTGG 1 cut(s) 794
BssECI CCNNGG 3 cut(s) 344, 865, 872
BssMI GATC 1 cut(s) 731
BssNAI GTATAC 1 cut(s) 811
BssNI GRCGYC 1 cut(s) 219
BssSI CACGAG 1 cut(s) 647
BssT1I CCWWGG 1 cut(s) 872
Bst1107I GTATAC 1 cut(s) 811
Bst2BI CACGAG 1 cut(s) 647
Bst2UI CCWGG 4 cut(s) 345, 611, 691, 867
Bst4CI ACNGT 2 cut(s) 584, 808
Bst6I CTCTTC 1 cut(s) 45
BstACI GRCGYC 1 cut(s) 219
BstC8I GCNNGC 1 cut(s) 463
BstDEI CTNAG 3 cut(s) 12, 243, 456
BstDSI CCRYGG 1 cut(s) 872
BstF5I GGATG 2 cut(s) 187, 355
BstKTI GATC 1 cut(s) 734
BstMAI GTCTC 3 cut(s) 145, 226, 797
BstMBI GATC 1 cut(s) 731
BstMWI GCNNNNNNNGC 2 cut(s) 170, 266
BstNI CCWGG 4 cut(s) 345, 611, 691, 867
BstSCI CCNGG 5 cut(s) 343, 434, 609, 689, 865
BstV1I GCAGC 7 cut(s) 46, 112, 115, 234, 397, 601, 757
BstX2I RGATCY 1 cut(s) 731
BstXI CCANNNNNNTGG 1 cut(s) 873
BstYI RGATCY 1 cut(s) 731
BstZ17I GTATAC 1 cut(s) 811
Bsu36I CCTNAGG 1 cut(s) 456
BsuRI GGCC 1 cut(s) 461
BtgI CCRYGG 1 cut(s) 872
BtsCI GGATG 2 cut(s) 187, 355
BtsIMutI CAGTG 1 cut(s) 142
Cac8I GCNNGC 1 cut(s) 463
Cfr13I GGNCC 3 cut(s) 438, 846, 869
CseI GACGC 2 cut(s) 200, 843
Csp6I GTAC 2 cut(s) 111, 341
CviAII CATG 1 cut(s) 873
CviQI GTAC 2 cut(s) 111, 341
DdeI CTNAG 3 cut(s) 12, 243, 456
DpnI GATC 1 cut(s) 733
DpnII GATC 1 cut(s) 731
Eam1104I CTCTTC 1 cut(s) 45
EarI CTCTTC 1 cut(s) 45
EciI GGCGGA 1 cut(s) 735
Eco130I CCWWGG 1 cut(s) 872
Eco47I GGWCC 3 cut(s) 438, 846, 869
Eco81I CCTNAGG 1 cut(s) 456
Eco88I CYCGRG 1 cut(s) 177
EcoO109I RGGNCCY 1 cut(s) 846
EcoRII CCWGG 4 cut(s) 343, 609, 689, 865
EcoT14I CCWWGG 1 cut(s) 872
ErhI CCWWGG 1 cut(s) 872
Esp3I CGTCTC 1 cut(s) 226
FaeI CATG 1 cut(s) 876
FaiI YATR 8 cut(s) 96, 383, 625, 728, 800, 811, 874, 932
FalI AAGNNNNNCTT 2 cut(s) 588, 620
FaqI GGGAC 2 cut(s) 846, 859
FatI CATG 1 cut(s) 872
FblI GTMKAC 1 cut(s) 810
Fnu4HI GCNGC 8 cut(s) 60, 100, 126, 129, 248, 386, 615, 771
FokI GGATG 2 cut(s) 194, 362
Fsp4HI GCNGC 8 cut(s) 60, 100, 126, 129, 248, 386, 615, 771
FspBI CTAG 1 cut(s) 144
GluI GCNGC 8 cut(s) 60, 100, 126, 129, 248, 386, 615, 771
HaeIII GGCC 1 cut(s) 461
HapII CCGG 1 cut(s) 436
HgaI GACGC 2 cut(s) 200, 843
Hin1I GRCGYC 1 cut(s) 219
Hin1II CATG 1 cut(s) 876
HinfI GANTC 2 cut(s) 335, 485
HpaII CCGG 1 cut(s) 436
Hpy166II GTNNAC 2 cut(s) 113, 811
Hpy188I TCNGA 4 cut(s) 13, 244, 525, 887
Hpy188III TCNNGA 4 cut(s) 144, 179, 327, 395
Hpy8I GTNNAC 2 cut(s) 113, 811
Hpy99I CGWCG 2 cut(s) 194, 940
HpyAV CCTTC 5 cut(s) 45, 280, 350, 491, 517
HpyCH4III ACNGT 2 cut(s) 584, 808
HpyCH4IV ACGT 2 cut(s) 219, 906
HpyCH4V TGCA 6 cut(s) 72, 131, 385, 479, 773, 916
HpyF10VI GCNNNNNNNGC 2 cut(s) 170, 266
HpyF3I CTNAG 3 cut(s) 12, 243, 456
HpySE526I ACGT 2 cut(s) 219, 906
Hsp92I GRCGYC 1 cut(s) 219
Hsp92II CATG 1 cut(s) 876
KflI GGGWCCC 1 cut(s) 846
Kzo9I GATC 1 cut(s) 731
LmnI GCTCC 1 cut(s) 500
Lsp1109I GCAGC 7 cut(s) 46, 112, 115, 234, 397, 601, 757
MaeI CTAG 1 cut(s) 144
MaeII ACGT 2 cut(s) 219, 906
MaeIII GTNAC 2 cut(s) 64, 404
MalI GATC 1 cut(s) 733
MboI GATC 1 cut(s) 731
MboII GAAGA 6 cut(s) 32, 442, 608, 668, 745, 956
MfeI CAATTG 1 cut(s) 73
MflI RGATCY 1 cut(s) 731
MluCI AATT 9 cut(s) 73, 155, 166, 225, 390, 398, 473, 699, 823
MnlI CCTC 6 cut(s) 7, 353, 451, 538, 836, 888
MroXI GAANNNNTTC 1 cut(s) 881
MseI TTAA 4 cut(s) 413, 471, 630, 828
MspI CCGG 1 cut(s) 436
MspR9I CCNGG 5 cut(s) 345, 436, 611, 691, 867
MunI CAATTG 1 cut(s) 73
Mva1269I GAATGC 1 cut(s) 131
MvaI CCWGG 4 cut(s) 345, 611, 691, 867
MwoI GCNNNNNNNGC 2 cut(s) 170, 266
NciI CCSGG 1 cut(s) 436
NcoI CCATGG 1 cut(s) 872
NdeII GATC 1 cut(s) 731
NlaIII CATG 1 cut(s) 876
NlaIV GGNNCC 5 cut(s) 18, 33, 695, 847, 848
PctI GAATGC 1 cut(s) 131
PdmI GAANNNNTTC 1 cut(s) 881
PfeI GAWTC 2 cut(s) 335, 485
PflMI CCANNNNNTGG 1 cut(s) 350
PfoI TCCNGGA 1 cut(s) 689
PkrI GCNGC 8 cut(s) 61, 101, 127, 130, 249, 387, 616, 772
PpuMI RGGWCCY 1 cut(s) 846
Psp5II RGGWCCY 1 cut(s) 846
Psp6I CCWGG 4 cut(s) 343, 609, 689, 865
PspGI CCWGG 4 cut(s) 343, 609, 689, 865
PspN4I GGNNCC 5 cut(s) 18, 33, 695, 847, 848
PspPI GGNCC 3 cut(s) 438, 846, 869
PspPPI RGGWCCY 1 cut(s) 846
PsuI RGATCY 1 cut(s) 731
RsaI GTAC 2 cut(s) 112, 342
RsaNI GTAC 2 cut(s) 111, 341
SaqAI TTAA 4 cut(s) 413, 471, 630, 828
SatI GCNGC 8 cut(s) 60, 100, 126, 129, 248, 386, 615, 771
Sau3AI GATC 1 cut(s) 731
Sau96I GGNCC 3 cut(s) 438, 846, 869
ScrFI CCNGG 5 cut(s) 345, 436, 611, 691, 867
SinI GGWCC 3 cut(s) 438, 846, 869
SmlI CTYRAG 1 cut(s) 941
SmoI CTYRAG 1 cut(s) 941
Sse9I AATT 9 cut(s) 73, 155, 166, 225, 390, 398, 473, 699, 823
SsiI CCGC 4 cut(s) 99, 409, 724, 746
SspI AATATT 1 cut(s) 447
SspMI CTAG 1 cut(s) 144
StyD4I CCNGG 5 cut(s) 343, 434, 609, 689, 865
StyI CCWWGG 1 cut(s) 872
TaaI ACNGT 2 cut(s) 584, 808
TaiI ACGT 2 cut(s) 222, 909
TaqI TCGA 1 cut(s) 394
TasI AATT 9 cut(s) 73, 155, 166, 225, 390, 398, 473, 699, 823
TatI WGTACW 1 cut(s) 110
TauI GCSGC 1 cut(s) 102
TfiI GAWTC 2 cut(s) 335, 485
Tru1I TTAA 4 cut(s) 413, 471, 630, 828
Tru9I TTAA 4 cut(s) 413, 471, 630, 828
TscAI CASTG 1 cut(s) 142
TseI GCWGC 7 cut(s) 59, 125, 128, 247, 385, 614, 770
TspDTI ATGAA 3 cut(s) 590, 615, 654
TspGWI ACGGA 1 cut(s) 9
TspRI CASTG 1 cut(s) 142
Van91I CCANNNNNTGG 1 cut(s) 350
VpaK11BI GGWCC 3 cut(s) 438, 846, 869
XapI RAATTY 4 cut(s) 155, 166, 398, 473
XbaI TCTAGA 1 cut(s) 143
XmiI GTMKAC 1 cut(s) 810
XmnI GAANNNNTTC 1 cut(s) 881
XspI CTAG 1 cut(s) 144
ZraI GACGTC 1 cut(s) 220
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.