MD00G1004400.v1.1

transcription regulatory region sequence-specific DNA binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0000758
Physical Location & Seq
Reverse (-)
681935 .. 683288
1354 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1004400.v1.1.491

Sequence Viewer

Length: 459 bp
ATGCAGCACTTGAAGCATGAATCAGCTACTATGGCCCAGAAGATAGAGATCCTAGAAGCTTCTCAACGGAAGTATTTGGGAAATGGTTTGGATTCTTGCTCTGTTGAAGAACTCCAAGAGATCAGTAGCCAGCTGGAACGAAGCTTATGCAACATAAGAGAGAGGAAGGCTCAATTATATATGGAGCAGGGGGAACAACTTAAGGCAAAGGAGAGGTTCCTGTTACAAGAGAATGCACAATTGTGGGAAGAGTGTCGTGCAAAGCCGTGGATGGAGTCTTCAGGTCAAGAAGAAAGCGCTTCTGCTTCTGCAAGCTATGAAAAAGCAGGAGCTTCTGCTTTTGTAAGCAACTGGAGCCAAAGAAGCATGAGTTCTAAGGTGGAGACCGAATTGCTCATTGGACCGCCAAGAGTGAACTGTTATTCGCATCCCAGTCTGAAGTCAGTATCAGATAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000060 GO:0001101 GO:0003006 GO:0003674 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006355 GO:0006606 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0007275 GO:0008104 GO:0008134 GO:0008150 GO:0009266 GO:0009409 GO:0009628 GO:0009719 GO:0009725 GO:0009739 GO:0009791 GO:0009888 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009909 GO:0009911 GO:0010033 GO:0010073 GO:0010074 GO:0010077 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0015031 GO:0015833 GO:0017038 GO:0019219 GO:0019222 GO:0019827 GO:0022414 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0033036 GO:0033365 GO:0033993 GO:0034504 GO:0034613 GO:0042221 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045184 GO:0045893 GO:0045935 GO:0046907 GO:0048367 GO:0048507 GO:0048518 GO:0048522 GO:0048580 GO:0048582 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051169 GO:0051170 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051239 GO:0051240 GO:0051252 GO:0051254 GO:0051641 GO:0051649 GO:0060255 GO:0061458 GO:0065007 GO:0070727 GO:0071702 GO:0071705 GO:0072594 GO:0080090 GO:0090567 GO:0098727 GO:0140110 GO:1901700 GO:1902680 GO:1903506 GO:1903508 GO:2000026 GO:2000112 GO:2000241 GO:2000243 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04013 ko04022 ko04371 ko05418 map04013 map04022 map04371 map05418
Pfam Domains
Protein Families
K-box SRF-TF

Protein Analysis

153

Amino Acids

17.2

Weight (kDa)

5.71

Isoelectric Point (pI)

64.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
K-box PF01486 2 - 84 2.5e-22 K-box region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 404
AclWI GGATC 1 cut(s) 43
AcuI CTGAAG 2 cut(s) 264, 458
AfeI AGCGCT 1 cut(s) 298
AflII CTTAAG 1 cut(s) 200
AgsI TTSAA 2 cut(s) 13, 107
AjuI GAANNNNNNNTTGG 2 cut(s) 381, 413
AleI CACNNNNGTG 1 cut(s) 241
AluBI AGCT 6 cut(s) 26, 59, 133, 144, 315, 332
AluI AGCT 6 cut(s) 26, 59, 133, 144, 315, 332
Alw26I GTCTC 1 cut(s) 377
AlwI GGATC 1 cut(s) 43
Aor51HI AGCGCT 1 cut(s) 298
AoxI GGCC 1 cut(s) 33
ApeKI GCWGC 1 cut(s) 4
AspLEI GCGC 1 cut(s) 299
AspS9I GGNCC 2 cut(s) 34, 401
AvaII GGWCC 1 cut(s) 401
BbsI GAAGAC 1 cut(s) 270
BbvI GCAGC 1 cut(s) 16
BccI CCATC 1 cut(s) 265
BceAI ACGGC 1 cut(s) 250
BcgI CGANNNNNNTGC 2 cut(s) 129, 163
BcoDI GTCTC 1 cut(s) 377
BfaI CTAG 1 cut(s) 53
BfoI RGCGCY 1 cut(s) 300
BfrI CTTAAG 1 cut(s) 200
BisI GCNGC 1 cut(s) 5
BlsI GCNGC 1 cut(s) 6
Bme18I GGWCC 1 cut(s) 401
BmgT120I GGNCC 2 cut(s) 34, 401
BmiI GGNNCC 2 cut(s) 218, 356
BmrI ACTGGG 1 cut(s) 426
BmsI GCATC 1 cut(s) 436
BmuI ACTGGG 1 cut(s) 426
BpiI GAAGAC 1 cut(s) 270
BplI GAGNNNNNCTC 2 cut(s) 154, 186
BpmI CTGGAG 1 cut(s) 373
BsaBI GATNNNNATC 1 cut(s) 47
BsaI GGTCTC 1 cut(s) 377
BsaJI CCNNGG 1 cut(s) 266
Bse1I ACTGG 2 cut(s) 356, 432
Bse8I GATNNNNATC 1 cut(s) 47
BseDI CCNNGG 1 cut(s) 266
BseGI GGATG 2 cut(s) 276, 427
BseJI GATNNNNATC 1 cut(s) 47
BseNI ACTGG 2 cut(s) 356, 432
BseXI GCAGC 1 cut(s) 16
BshFI GGCC 1 cut(s) 35
BsmAI GTCTC 1 cut(s) 377
BsmI GAATGC 1 cut(s) 238
BsnI GGCC 1 cut(s) 35
Bso31I GGTCTC 1 cut(s) 377
Bsp143I GATC 2 cut(s) 48, 120
BspACI CCGC 1 cut(s) 404
BspANI GGCC 1 cut(s) 35
BspLI GGNNCC 2 cut(s) 218, 356
BspPI GGATC 1 cut(s) 43
BspTI CTTAAG 1 cut(s) 200
BspTNI GGTCTC 1 cut(s) 377
BsrI ACTGG 2 cut(s) 356, 432
BssECI CCNNGG 1 cut(s) 266
BssMI GATC 2 cut(s) 48, 120
Bst4CI ACNGT 1 cut(s) 419
Bst6I CTCTTC 1 cut(s) 243
BstAFI CTTAAG 1 cut(s) 200
BstC8I GCNNGC 2 cut(s) 131, 313
BstDEI CTNAG 1 cut(s) 375
BstDSI CCRYGG 1 cut(s) 266
BstF5I GGATG 2 cut(s) 276, 427
BstH2I RGCGCY 1 cut(s) 300
BstHHI GCGC 1 cut(s) 299
BstKTI GATC 2 cut(s) 51, 123
BstMAI GTCTC 1 cut(s) 377
BstMBI GATC 2 cut(s) 48, 120
BstMWI GCNNNNNNNGC 4 cut(s) 13, 32, 354, 363
BstV1I GCAGC 1 cut(s) 16
BstV2I GAAGAC 1 cut(s) 270
BstX2I RGATCY 1 cut(s) 48
BstYI RGATCY 1 cut(s) 48
BsuRI GGCC 1 cut(s) 35
BtgI CCRYGG 1 cut(s) 266
BtsCI GGATG 2 cut(s) 276, 427
Cac8I GCNNGC 2 cut(s) 131, 313
CfoI GCGC 1 cut(s) 299
Cfr13I GGNCC 2 cut(s) 34, 401
CviAII CATG 2 cut(s) 17, 367
DdeI CTNAG 1 cut(s) 375
DpnI GATC 2 cut(s) 50, 122
DpnII GATC 2 cut(s) 48, 120
Eam1104I CTCTTC 1 cut(s) 243
EarI CTCTTC 1 cut(s) 243
Eco31I GGTCTC 1 cut(s) 377
Eco47I GGWCC 1 cut(s) 401
Eco47III AGCGCT 1 cut(s) 298
Eco57I CTGAAG 2 cut(s) 264, 458
FaeI CATG 2 cut(s) 20, 370
FaiI YATR 9 cut(s) 18, 32, 148, 155, 178, 180, 182, 318, 368
FatI CATG 2 cut(s) 16, 366
Fnu4HI GCNGC 1 cut(s) 5
FokI GGATG 2 cut(s) 283, 414
Fsp4HI GCNGC 1 cut(s) 5
FspBI CTAG 1 cut(s) 53
GlaI GCGC 1 cut(s) 298
GluI GCNGC 1 cut(s) 5
GsuI CTGGAG 1 cut(s) 373
HaeII RGCGCY 1 cut(s) 300
HaeIII GGCC 1 cut(s) 35
HhaI GCGC 1 cut(s) 299
Hin1II CATG 2 cut(s) 20, 370
Hin6I GCGC 1 cut(s) 297
HinP1I GCGC 1 cut(s) 297
HindIII AAGCTT 2 cut(s) 57, 142
HinfI GANTC 3 cut(s) 20, 92, 275
Hpy166II GTNNAC 1 cut(s) 415
Hpy188I TCNGA 2 cut(s) 438, 451
Hpy188III TCNNGA 1 cut(s) 287
Hpy8I GTNNAC 1 cut(s) 415
HpyAV CCTTC 1 cut(s) 160
HpyCH4III ACNGT 1 cut(s) 419
HpyCH4V TGCA 5 cut(s) 4, 150, 236, 260, 311
HpyF10VI GCNNNNNNNGC 4 cut(s) 13, 32, 354, 363
HpyF3I CTNAG 1 cut(s) 375
Hsp92II CATG 2 cut(s) 20, 370
HspAI GCGC 1 cut(s) 297
Kzo9I GATC 2 cut(s) 48, 120
LmnI GCTCC 3 cut(s) 184, 329, 354
LpnPI CCDG 9 cut(s) 50, 119, 143, 173, 233, 267, 312, 337, 445
Lsp1109I GCAGC 1 cut(s) 16
LweI GCATC 1 cut(s) 436
MaeI CTAG 1 cut(s) 53
MaeIII GTNAC 1 cut(s) 222
MalI GATC 2 cut(s) 50, 122
MboI GATC 2 cut(s) 48, 120
MboII GAAGA 5 cut(s) 52, 119, 260, 270, 302
MfeI CAATTG 1 cut(s) 239
MflI RGATCY 1 cut(s) 48
MluCI AATT 3 cut(s) 173, 239, 389
MlyI GAGTC 1 cut(s) 284
MnlI CCTC 2 cut(s) 156, 207
MseI TTAA 1 cut(s) 201
MslI CAYNNNNRTG 1 cut(s) 241
MspA1I CMGCKG 1 cut(s) 133
MspCI CTTAAG 1 cut(s) 200
MunI CAATTG 1 cut(s) 239
Mva1269I GAATGC 1 cut(s) 238
MwoI GCNNNNNNNGC 4 cut(s) 13, 32, 354, 363
NdeII GATC 2 cut(s) 48, 120
NlaIII CATG 2 cut(s) 20, 370
NlaIV GGNNCC 2 cut(s) 218, 356
OliI CACNNNNGTG 1 cut(s) 241
PctI GAATGC 1 cut(s) 238
PfeI GAWTC 2 cut(s) 20, 92
PkrI GCNGC 1 cut(s) 6
PleI GAGTC 1 cut(s) 283
PpsI GAGTC 1 cut(s) 283
PspN4I GGNNCC 2 cut(s) 218, 356
PspPI GGNCC 2 cut(s) 34, 401
PsuI RGATCY 1 cut(s) 48
PvuII CAGCTG 1 cut(s) 133
RseI CAYNNNNRTG 1 cut(s) 241
SaqAI TTAA 1 cut(s) 201
SatI GCNGC 1 cut(s) 5
Sau3AI GATC 2 cut(s) 48, 120
Sau96I GGNCC 2 cut(s) 34, 401
SchI GAGTC 1 cut(s) 284
SetI ASST 9 cut(s) 28, 61, 135, 146, 218, 286, 317, 334, 381
SfaNI GCATC 1 cut(s) 436
SinI GGWCC 1 cut(s) 401
SmiMI CAYNNNNRTG 1 cut(s) 241
SmlI CTYRAG 1 cut(s) 200
SmoI CTYRAG 1 cut(s) 200
Sse9I AATT 3 cut(s) 173, 239, 389
SsiI CCGC 1 cut(s) 404
SspMI CTAG 1 cut(s) 53
TaaI ACNGT 1 cut(s) 419
TaqII GACCGA 1 cut(s) 401
TasI AATT 3 cut(s) 173, 239, 389
TfiI GAWTC 2 cut(s) 20, 92
Tru1I TTAA 1 cut(s) 201
Tru9I TTAA 1 cut(s) 201
TseI GCWGC 1 cut(s) 4
TspDTI ATGAA 2 cut(s) 33, 333
TspGWI ACGGA 1 cut(s) 82
Vha464I CTTAAG 1 cut(s) 200
VpaK11BI GGWCC 1 cut(s) 401
XspI CTAG 1 cut(s) 53
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.