Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0003960

Physical Location & Seq

Genomic Coordinates Forward (+)

20429650 .. 20431579

Gene Structure 1930 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1097800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1728 bp

ATGGCAGAACAGATTGTATGCTTTGTGATCGACAAGTTAATTCAACTACTCATCGCCACAGAAGCAAACCTTTCGAGAAATGCACGTACTGAAGTTGGTTTCATCAAAGATGAACTCGAAAGCATCCGATCATTCCTCAAGGAAGCAGATGCTATGGCTGCAGCAGCAGATGGAGAGACGGGGAATGACGGTGTCAAAACTTGGGTCAATCAAGTAAGAGAAGCAGCTTTTTACATTGAAGATGTCATTGATGAATATTTGCTCCGCATCTTATGGCATCATCAACACCATGGATTCCTTCGTTTTTTCCACAAAATTATCCGGTTGGCTAAAAAAATGAGATCAAAGGATGGGATAGTTTCGAAAGTTGACAGTATAAAAAAATTGGTTCGTGAAATCAAGGAGAGAAGTGAAAGATATGGATTCAAATCCATTGATCAGCAAGGCCAGAGCAGTGGAGAAAAGACTCTACTGTCGAACGACCCTCGAGTGGCTTCCCTTTTCATTAAGGAAGCTGAAATTGTGGGGATTGAATCTGCCAGAGATGAGCTAACTCGTTTGTTGGTAGAAGGAGCGACGAGACGTGAAGTGATATCAGTGGTTGGCATGGGAGGACTTGGGAAAACCACTCTTGCCAAGAAAGTGTATGACCAGCCGAAGGTGATGGCACACTTTGATTGCTATGCTTGGATTACAGTGTCCCAGTCGTACAGGGTTGAGGATCTTCTGAGGACGGTAATCAAAAAGTTCTACAGTTCAAGGAAGGAGCGTTTCCCAGAGGAAATTGACACAATGGATGAGGAGTCCCTGATTAGCACATCGAGAGAGTATTTGCAGCAAAAGAGGAAGGCATTTCAGTTTGAATTGGAGGGAAAGTGTCCTGAAGAGTTGGAAGAGTTGTCTCTTAACATCGTCAAAAGATGCGAAGGACTACCTTTGGCAATTGTTTCCGTAGGTGGTCTGCTGTCAACCAAAGCTAAAGTTGTGTCTGAATGGCAGAAGTTATACAATAGCTTAAGTTCAGAGTTAGAAAGCAATCCCCACCTTACAAGCCTCACAAGAATCCTATCCCTCAGCTATCACCATCTACCGTATTACCTCAAATCTTGTGTATTATACTTTGGCATCTTTCCTGAGGATTACTCAGTTAGTTGCATAAGATTAGTTCGGTTGTGGATCGCTGAAGGCTTTGTGAAACCGAAGATGGGCAAGACACTAGAAGAGGTTGGAGAGGAATACTTGATGGAGCTAATTCACAGAAGCCTGGTTCAAGTTTCAGAAGTTTACATTGATGGAAAAGCTCGAGGTTGTAGAGTTCATGATCTATTGCACGACGTCCTCCTTAAAAAGGGGTTGGATTCCAGCTTCTGTCATGTGTTATCAAAAGGCAAGTTGACTTTTAAACCAACAACTCGACGTCTCTCAATGGACATCAATCCCTCTGAGGCCTTGGAAAGCATTACACAATTTCATATTCGTTCTGTATTTACTTTCAACCTAGAAGAATGGCCCGAGTCTTTTCTCGATACATTGAGTGGAAACTTTAAACTTCTGAAAGTATTGGATTTCACGGCTGCTCCAATCAATCAACTTCCTAAATATGTGGGGGATTTATATCTCTTGACGTACTTAAGCCTGAGGAATACAAAGGTGAAGTTGCTTCCAGACTCCATAGGCAATCTACAAAACTTGGAAACCCTGGATCTAAAAACAGTCTCTGGTGTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0002376 GO:0003674 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0008219 GO:0009626 GO:0009987 GO:0012501 GO:0016020 GO:0019897 GO:0019898 GO:0023052 GO:0033554 GO:0034050 GO:0036094 GO:0044425 GO:0044459 GO:0044464 GO:0045087 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071944 GO:0097159 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko04626 map04626

Pfam Domains

Protein Families

NB-ARC

Protein Analysis

576

Amino Acids

65.6

Weight (kDa)

6.94

Isoelectric Point (pI)

38.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Rx_N	PF18052	5 - 104	7.8e-21	Rx N-terminal domain
NB-ARC	PF00931	181 - 282	4.3e-19	NB-ARC domain
WHD_DRP	PF23559	376 - 446	4.7e-25	Disease resistance protein Winged helix domain
LRR_14	PF23598	491 - 571	4.6e-12	Leucine-rich repeat region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003960)

Species	Orthologous Gene IDs
malus_domestica	MD00G1097800.v1.1 MD01G1186500.v1.1 MD01G1186600.v1.1 MD01G1186800.v1.1 MD01G1187000.v1.1 MD01G1187100.v1.1 MD07G1258600.v1.1 MD07G1258700.v1.1 MD07G1258800.v1.1 MD07G1258900.v1.1 MD07G1259200.v1.1 MD07G1259300.v1.1
prunus_persica	Prupe.2G283200_v2.0.a1 Prupe.2G283300_v2.0.a1 Prupe.2G283400_v2.0.a1
pyrus_communis	pycom01g19810 pycom01g19820 pycom01g19840 pycom01g19900 pycom07g23170 pycom07g23230 pycom07g23240 pycom07g23250 pycom07g23260
rosa_chinensis	RchiOBHm_Chr1g0375241
rosa_laevigata	RLG00000026676
rosa_multiflora	Rmu_co8330553.1_g000001 Rmu_sc0000554.1_g000032
rosa_roxburghii	Rroxscaffold_4G00282910
rosa_rugosa	Rorug01G0389400
rosa_samantha	Rh1AG400100 Rh1BG364400 Rh1DG395000
rosa_wichuraiana	Rw1G035520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	472
AatII	GACGTC	2 cut(s)	1338, 1420
AciI	CCGC	1 cut(s)	265
AclWI	GGATC	3 cut(s)	729, 1184, 1710
AcuI	CTGAAG	3 cut(s)	111, 903, 1203
AcyI	GRCGYC	2 cut(s)	1335, 1417
AfaI	GTAC	3 cut(s)	88, 710, 1628
AfiI	CCNNNNNNNGG	4 cut(s)	490, 658, 1205, 1348
AflII	CTTAAG	2 cut(s)	1015, 1630
AgsI	TTSAA	8 cut(s)	44, 239, 427, 533, 759, 863, 1271, 1495
AjiI	CACGTC	1 cut(s)	584
AjnI	CCWGG	2 cut(s)	1263, 1698
AluBI	AGCT	9 cut(s)	227, 515, 550, 977, 1014, 1077, 1249, 1301, 1365
AluI	AGCT	9 cut(s)	227, 515, 550, 977, 1014, 1077, 1249, 1301, 1365
Alw26I	GTCTC	5 cut(s)	170, 574, 906, 1424, 1720
AlwI	GGATC	3 cut(s)	729, 1184, 1710
AlwNI	CAGNNNCTG	2 cut(s)	1368, 1718
Ama87I	CYCGRG	3 cut(s)	486, 1302, 1511
AoxI	GGCC	3 cut(s)	445, 1446, 1508
ApeKI	GCWGC	6 cut(s)	158, 161, 164, 224, 835, 1574
ArsI	GACNNNNNNTTYG	2 cut(s)	189, 221
AspS9I	GGNCC	1 cut(s)	1509
AsuHPI	GGTGA	3 cut(s)	673, 1073, 1663
AsuII	TTCGAA	1 cut(s)	362
AvaI	CYCGRG	3 cut(s)	486, 1302, 1511
AxyI	CCTNAGG	2 cut(s)	1134, 1637
BbvCI	CCTCAGC	1 cut(s)	1073
BbvI	GCAGC	6 cut(s)	145, 173, 176, 236, 847, 1561
BccI	CCATC	7 cut(s)	164, 344, 658, 1092, 1198, 1237, 1286
BceAI	ACGGC	1 cut(s)	1587
BcgI	CGANNNNNNTGC	2 cut(s)	54, 88
BciT130I	CCWGG	2 cut(s)	1265, 1700
BclI	TGATCA	1 cut(s)	436
BcoDI	GTCTC	5 cut(s)	170, 574, 906, 1424, 1720
BfaI	CTAG	2 cut(s)	1217, 1499
BfmI	CTRYAG	2 cut(s)	159, 751
BfrI	CTTAAG	2 cut(s)	1015, 1630
BisI	GCNGC	6 cut(s)	159, 162, 165, 225, 836, 1575
BlsI	GCNGC	6 cut(s)	160, 163, 166, 226, 837, 1576
Bme1390I	CCNGG	2 cut(s)	1265, 1700
BmeT110I	CYCGRG	3 cut(s)	486, 1302, 1511
BmgBI	CACGTC	1 cut(s)	584
BmgT120I	GGNCC	1 cut(s)	1509
BmrFI	CCNGG	2 cut(s)	1265, 1700
BmrI	ACTGGG	1 cut(s)	697
BmsI	GCATC	6 cut(s)	132, 139, 276, 286, 911, 1134
BmuI	ACTGGG	1 cut(s)	697
BplI	GAGNNNNNCTC	2 cut(s)	1127, 1159
Bpu10I	CCTNAGC	1 cut(s)	1073
Bpu14I	TTCGAA	1 cut(s)	362
BpuEI	CTTGAG	1 cut(s)	122
BsaAI	YACGTR	1 cut(s)	86
BsaBI	GATNNNNATC	2 cut(s)	427, 1614
BsaHI	GRCGYC	2 cut(s)	1335, 1417
BsaJI	CCNNGG	3 cut(s)	289, 1449, 1698
BsaWI	WCCGGW	1 cut(s)	321
BsaXI	ACNNNNNCTCC	4 cut(s)	860, 890, 1561, 1591
Bsc4I	CCNNNNNNNGG	4 cut(s)	490, 658, 1205, 1348
Bse1I	ACTGG	1 cut(s)	703
Bse21I	CCTNAGG	2 cut(s)	1134, 1637
Bse8I	GATNNNNATC	2 cut(s)	427, 1614
BseBI	CCWGG	2 cut(s)	1265, 1700
BseDI	CCNNGG	3 cut(s)	289, 1449, 1698
BseGI	GGATG	3 cut(s)	123, 355, 802
BseJI	GATNNNNATC	2 cut(s)	427, 1614
BseLI	CCNNNNNNNGG	4 cut(s)	490, 658, 1205, 1348
BseMII	CTCAG	6 cut(s)	719, 1087, 1125, 1158, 1434, 1628
BseNI	ACTGG	1 cut(s)	703
BseRI	GAGGAG	1 cut(s)	815
BseXI	GCAGC	6 cut(s)	145, 173, 176, 236, 847, 1561
BshFI	GGCC	3 cut(s)	447, 1448, 1510
BsiHKCI	CYCGRG	3 cut(s)	486, 1302, 1511
BsiSI	CCGG	1 cut(s)	322
BslFI	GGGAC	2 cut(s)	685, 790
BslI	CCNNNNNNNGG	4 cut(s)	490, 658, 1205, 1348
BsmAI	GTCTC	5 cut(s)	170, 574, 906, 1424, 1720
BsmBI	CGTCTC	3 cut(s)	170, 574, 1424
BsmFI	GGGAC	2 cut(s)	685, 790
BsnI	GGCC	3 cut(s)	447, 1448, 1510
BsoBI	CYCGRG	3 cut(s)	486, 1302, 1511
Bsp119I	TTCGAA	1 cut(s)	362
Bsp143I	GATC	8 cut(s)	27, 128, 341, 436, 721, 1176, 1321, 1702
Bsp19I	CCATGG	1 cut(s)	289
BspACI	CCGC	1 cut(s)	265
BspANI	GGCC	3 cut(s)	447, 1448, 1510
BspCNI	CTCAG	6 cut(s)	720, 1086, 1126, 1157, 1435, 1629
BspHI	TCATGA	1 cut(s)	1318
BspMAI	CTGCAG	1 cut(s)	163
BspPI	GGATC	3 cut(s)	729, 1184, 1710
BspT104I	TTCGAA	1 cut(s)	362
BspTI	CTTAAG	2 cut(s)	1015, 1630
BsrI	ACTGG	1 cut(s)	703
BssECI	CCNNGG	3 cut(s)	289, 1449, 1698
BssMI	GATC	8 cut(s)	27, 128, 341, 436, 721, 1176, 1321, 1702
BssNI	GRCGYC	2 cut(s)	1335, 1417
BssT1I	CCWWGG	2 cut(s)	289, 1449
Bst2UI	CCWGG	2 cut(s)	1265, 1700
Bst4CI	ACNGT	8 cut(s)	191, 374, 474, 697, 736, 755, 1092, 1714
Bst6I	CTCTTC	3 cut(s)	879, 888, 1215
BstACI	GRCGYC	2 cut(s)	1335, 1417
BstAFI	CTTAAG	2 cut(s)	1015, 1630
BstBAI	YACGTR	1 cut(s)	86
BstBI	TTCGAA	1 cut(s)	362
BstDEI	CTNAG	6 cut(s)	728, 1073, 1134, 1144, 1443, 1637
BstDSI	CCRYGG	1 cut(s)	289
BstENI	CCTNNNNNAGG	1 cut(s)	1346
BstF5I	GGATG	3 cut(s)	123, 355, 802
BstKTI	GATC	8 cut(s)	30, 131, 344, 439, 724, 1179, 1324, 1705
BstMAI	GTCTC	5 cut(s)	170, 574, 906, 1424, 1720
BstMBI	GATC	8 cut(s)	27, 128, 341, 436, 721, 1176, 1321, 1702
BstMWI	GCNNNNNNNGC	3 cut(s)	62, 158, 164
BstNI	CCWGG	2 cut(s)	1265, 1700
BstSCI	CCNGG	2 cut(s)	1263, 1698
BstSFI	CTRYAG	2 cut(s)	159, 751
BstV1I	GCAGC	6 cut(s)	145, 173, 176, 236, 847, 1561
BstX2I	RGATCY	2 cut(s)	721, 1702
BstXI	CCANNNNNNTGG	1 cut(s)	455
BstYI	RGATCY	2 cut(s)	721, 1702
Bsu36I	CCTNAGG	2 cut(s)	1134, 1637
BsuRI	GGCC	3 cut(s)	447, 1448, 1510
BtgI	CCRYGG	1 cut(s)	289
BtgZI	GCGATG	1 cut(s)	37
BtrI	CACGTC	1 cut(s)	584
BtsCI	GGATG	3 cut(s)	123, 355, 802
BtsI	GCAGTG	1 cut(s)	460
BtsIMutI	CAGTG	3 cut(s)	460, 603, 702
CaiI	CAGNNNCTG	2 cut(s)	1368, 1718
CciI	TCATGA	1 cut(s)	1318
Cfr13I	GGNCC	1 cut(s)	1509
Csp6I	GTAC	3 cut(s)	87, 709, 1627
CviAII	CATG	4 cut(s)	290, 607, 1319, 1373
CviQI	GTAC	3 cut(s)	87, 709, 1627
DdeI	CTNAG	6 cut(s)	728, 1073, 1134, 1144, 1443, 1637
DpnI	GATC	8 cut(s)	29, 130, 343, 438, 723, 1178, 1323, 1704
DpnII	GATC	8 cut(s)	27, 128, 341, 436, 721, 1176, 1321, 1702
DraI	TTTAAA	2 cut(s)	1402, 1546
DrdI	GACNNNNNNGTC	1 cut(s)	472
DseDI	GACNNNNNNGTC	1 cut(s)	472
Eam1104I	CTCTTC	3 cut(s)	879, 888, 1215
EarI	CTCTTC	3 cut(s)	879, 888, 1215
Eco130I	CCWWGG	2 cut(s)	289, 1449
Eco147I	AGGCCT	1 cut(s)	1448
Eco32I	GATATC	1 cut(s)	594
Eco57I	CTGAAG	3 cut(s)	111, 903, 1203
Eco81I	CCTNAGG	2 cut(s)	1134, 1637
Eco88I	CYCGRG	3 cut(s)	486, 1302, 1511
EcoNI	CCTNNNNNAGG	1 cut(s)	1346
EcoRII	CCWGG	2 cut(s)	1263, 1698
EcoRV	GATATC	1 cut(s)	594
EcoT14I	CCWWGG	2 cut(s)	289, 1449
ErhI	CCWWGG	2 cut(s)	289, 1449
Esp3I	CGTCTC	3 cut(s)	170, 574, 1424
FaeI	CATG	4 cut(s)	293, 610, 1322, 1376
FalI	AAGNNNNNCTT	2 cut(s)	54, 86
FaqI	GGGAC	2 cut(s)	685, 790
FatI	CATG	4 cut(s)	289, 606, 1318, 1372
FbaI	TGATCA	1 cut(s)	436
Fnu4HI	GCNGC	6 cut(s)	159, 162, 165, 225, 836, 1575
FokI	GGATG	3 cut(s)	110, 362, 809
Fsp4HI	GCNGC	6 cut(s)	159, 162, 165, 225, 836, 1575
FspBI	CTAG	2 cut(s)	1217, 1499
GluI	GCNGC	6 cut(s)	159, 162, 165, 225, 836, 1575
HaeIII	GGCC	3 cut(s)	447, 1448, 1510
HapII	CCGG	1 cut(s)	322
Hin1I	GRCGYC	2 cut(s)	1335, 1417
Hin1II	CATG	4 cut(s)	293, 610, 1322, 1376
HincII	GTYRAC	3 cut(s)	370, 969, 1395
HindII	GTYRAC	3 cut(s)	370, 969, 1395
HinfI	GANTC	9 cut(s)	294, 423, 466, 533, 803, 1062, 1358, 1514, 1667
HpaII	CCGG	1 cut(s)	322
HphI	GGTGA	3 cut(s)	673, 1073, 1663
Hpy166II	GTNNAC	4 cut(s)	370, 969, 1285, 1395
Hpy188I	TCNGA	7 cut(s)	128, 729, 991, 1024, 1279, 1444, 1554
Hpy188III	TCNNGA	9 cut(s)	75, 392, 822, 881, 1133, 1319, 1523, 1621, 1664
Hpy8I	GTNNAC	4 cut(s)	370, 969, 1285, 1395
Hpy99I	CGWCG	3 cut(s)	580, 1337, 1419
HpyAV	CCTTC	7 cut(s)	308, 563, 652, 757, 841, 920, 1178
HpyCH4III	ACNGT	8 cut(s)	191, 374, 474, 697, 736, 755, 1092, 1714
HpyCH4IV	ACGT	5 cut(s)	85, 583, 1335, 1417, 1625
HpyCH4V	TGCA	5 cut(s)	83, 161, 835, 1155, 1330
HpyF10VI	GCNNNNNNNGC	3 cut(s)	62, 158, 164
HpyF3I	CTNAG	6 cut(s)	728, 1073, 1134, 1144, 1443, 1637
HpySE526I	ACGT	5 cut(s)	85, 583, 1335, 1417, 1625
Hsp92I	GRCGYC	2 cut(s)	1335, 1417
Hsp92II	CATG	4 cut(s)	293, 610, 1322, 1376
Ksp22I	TGATCA	1 cut(s)	436
Kzo9I	GATC	8 cut(s)	27, 128, 341, 436, 721, 1176, 1321, 1702
LmnI	GCTCC	5 cut(s)	267, 572, 766, 1246, 1582
Lsp1109I	GCAGC	6 cut(s)	145, 173, 176, 236, 847, 1561
LweI	GCATC	6 cut(s)	132, 139, 276, 286, 911, 1134
MaeI	CTAG	2 cut(s)	1217, 1499
MaeII	ACGT	5 cut(s)	85, 583, 1335, 1417, 1625
MalI	GATC	8 cut(s)	29, 130, 343, 438, 723, 1178, 1323, 1704
MboI	GATC	8 cut(s)	27, 128, 341, 436, 721, 1176, 1321, 1702
MboII	GAAGA	7 cut(s)	251, 716, 896, 905, 1213, 1232, 1514
MfeI	CAATTG	1 cut(s)	942
MflI	RGATCY	2 cut(s)	721, 1702
MluCI	AATT	9 cut(s)	39, 315, 383, 519, 783, 863, 942, 1251, 1466
MlyI	GAGTC	4 cut(s)	460, 812, 1523, 1661
MmeI	TCCRAC	3 cut(s)	870, 1207, 1335
MseI	TTAA	8 cut(s)	38, 507, 906, 1016, 1344, 1401, 1545, 1631
MspCI	CTTAAG	2 cut(s)	1015, 1630
MspI	CCGG	1 cut(s)	322
MspR9I	CCNGG	2 cut(s)	1265, 1700
MunI	CAATTG	1 cut(s)	942
MvaI	CCWGG	2 cut(s)	1265, 1700
MwoI	GCNNNNNNNGC	3 cut(s)	62, 158, 164
NcoI	CCATGG	1 cut(s)	289
NdeII	GATC	8 cut(s)	27, 128, 341, 436, 721, 1176, 1321, 1702
NlaIII	CATG	4 cut(s)	293, 610, 1322, 1376
NspV	TTCGAA	1 cut(s)	362
PaeR7I	CTCGAG	2 cut(s)	486, 1302
PagI	TCATGA	1 cut(s)	1318
PceI	AGGCCT	1 cut(s)	1448
PfeI	GAWTC	5 cut(s)	294, 423, 533, 1062, 1358
PflFI	GACNNNGTC	1 cut(s)	191
PkrI	GCNGC	6 cut(s)	160, 163, 166, 226, 837, 1576
PleI	GAGTC	4 cut(s)	460, 811, 1522, 1661
PpsI	GAGTC	4 cut(s)	460, 811, 1522, 1661
Ppu21I	YACGTR	1 cut(s)	86
Psp6I	CCWGG	2 cut(s)	1263, 1698
PspGI	CCWGG	2 cut(s)	1263, 1698
PspPI	GGNCC	1 cut(s)	1509
PspXI	VCTCGAGB	2 cut(s)	486, 1302
PstI	CTGCAG	1 cut(s)	163
PstNI	CAGNNNCTG	2 cut(s)	1368, 1718
PsuI	RGATCY	2 cut(s)	721, 1702
PsyI	GACNNNGTC	1 cut(s)	191
RsaI	GTAC	3 cut(s)	88, 710, 1628
RsaNI	GTAC	3 cut(s)	87, 709, 1627
SaqAI	TTAA	8 cut(s)	38, 507, 906, 1016, 1344, 1401, 1545, 1631
SatI	GCNGC	6 cut(s)	159, 162, 165, 225, 836, 1575
Sau3AI	GATC	8 cut(s)	27, 128, 341, 436, 721, 1176, 1321, 1702
Sau96I	GGNCC	1 cut(s)	1509
SchI	GAGTC	4 cut(s)	460, 812, 1523, 1661
ScrFI	CCNGG	2 cut(s)	1265, 1700
SfaNI	GCATC	6 cut(s)	132, 139, 276, 286, 911, 1134
SfcI	CTRYAG	2 cut(s)	159, 751
Sfr274I	CTCGAG	2 cut(s)	486, 1302
SfuI	TTCGAA	1 cut(s)	362
SlaI	CTCGAG	2 cut(s)	486, 1302
SmlI	CTYRAG	5 cut(s)	137, 486, 1015, 1302, 1630
SmoI	CTYRAG	5 cut(s)	137, 486, 1015, 1302, 1630
Sse9I	AATT	9 cut(s)	39, 315, 383, 519, 783, 863, 942, 1251, 1466
SseBI	AGGCCT	1 cut(s)	1448
SsiI	CCGC	1 cut(s)	265
SspI	AATATT	1 cut(s)	257
SspMI	CTAG	2 cut(s)	1217, 1499
StuI	AGGCCT	1 cut(s)	1448
StyD4I	CCNGG	2 cut(s)	1263, 1698
StyI	CCWWGG	2 cut(s)	289, 1449
TaaI	ACNGT	8 cut(s)	191, 374, 474, 697, 736, 755, 1092, 1714
TaiI	ACGT	5 cut(s)	88, 586, 1338, 1420, 1628
TasI	AATT	9 cut(s)	39, 315, 383, 519, 783, 863, 942, 1251, 1466
TfiI	GAWTC	5 cut(s)	294, 423, 533, 1062, 1358
Tru1I	TTAA	8 cut(s)	38, 507, 906, 1016, 1344, 1401, 1545, 1631
Tru9I	TTAA	8 cut(s)	38, 507, 906, 1016, 1344, 1401, 1545, 1631
TscAI	CASTG	3 cut(s)	460, 603, 702
TseI	GCWGC	6 cut(s)	158, 161, 164, 224, 835, 1574
TspDTI	ATGAA	6 cut(s)	91, 126, 267, 493, 1307, 1460
TspGWI	ACGGA	1 cut(s)	940
TspRI	CASTG	3 cut(s)	460, 603, 702
Tth111I	GACNNNGTC	1 cut(s)	191
Vha464I	CTTAAG	2 cut(s)	1015, 1630
XagI	CCTNNNNNAGG	1 cut(s)	1346
XhoI	CTCGAG	2 cut(s)	486, 1302
XspI	CTAG	2 cut(s)	1217, 1499
ZraI	GACGTC	2 cut(s)	1336, 1418

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.