Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0003960

Physical Location & Seq

Genomic Coordinates Forward (+)

28581293 .. 28582920

Gene Structure 1628 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1187100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1608 bp

ATGGCAGAACAGATTGTATGCTTTGTGATCGACAAGTTAATTCAACTACTCATCGCCACAGAAGCAAACCTTTCGAGAAATGCACGTACTGAAGTTGGTTTCATCAAAGATGAACTCGAAAGCATCCGATCATTCCTCAAGGAAGCAGATGCTATGGCTGCAGCAGCAGATGGAGAGACGGGGAATGACGGTGTCAAAACTTGGGTCAATCAAGTAAGAGAAGCAGCTTTTTACATTGAAGATGTCATTGATGAATATTTGCTCCGCATCTTATGGCATCATCAACACACCATGGATTCCTTCGTTTTTTTCCACAAAATTATCCGGTTGGCTAAAAAAATGAGATCAAAGGATGGGATAGTTTCGAAAGTTGACAGTATAAAAAAATTGGTTCGTGAAATCAAGGAGAGAAGTGAAAGATATGGATTCAAATCCATTGATCAGCAAGGCCAGAGCAGTGGAGAAAAGACTCTACTGTCGAACGACCCTCGAGTGGCTTCCCTTTTCATTAAGGAAGCTGAAATTGTGGGGATTGAATCTGCCAGAGATGAGCTAACTCGTTTGTTGGTAGAAGGAGCGACGAGACGTGAAGTGATATCAGTGGTTGGCATGGGAGGACTTGGGAAAACCACTCTTGCCAAGAAAGTGTATGACCAGCCGAAGGTGATGGCACACTTTGATTGCTATGCTTGGATTACAGTGTCCCAGTCGTACAGGGTTGAGGATCTTCTGAGGACGGTAATCAAAAAGTTCTACAGTTCAAGGAAGGAGCGTTTCCCAGAGGAAATTGACACAATGGATGAGGAGTCCCTGATTAGCACATCGAGAGAGTATTTGCAGCAAAAGAGGTACATTGTTGTGTTTGATGATGTGTGGAAAGTAGATTTTTGGGGGGCAATAGAACATGCTTTACCCGATAATGAAGGCGGACGAATAATTATCACTACACGGATTCAAGATGTTGCCAACTTTTGCAAAAGATCTTGTTTTGTTCATGTTCATCACTTGCAGCATCTGCCTCCAAACAAGGCATGGAAATTGTTTTGTAGGAAGGCATTTCAGTTTGAATTGGAGGGAAAGTGTCCTGAAGAGTTGGAAGAGTTGTCTCTTAACATCGTCAAAAGATGCGAAGGACTACCTTTGGCAATTGTTTCCGTAGGTGGTCTGCTGTCAACCAAAGCTAAAGTTGTGTCTGAATGGCAGAAGTTATACAATAGCTTAAGTTCAGAGTTAGAAAGCAATCCCCACCTTACAAGCCTCACAAGAATCCTATCCCTCAGCTATCACCATCTACCGTATTACCTCAAATCTTGTGTATTATACTTTGGCATCTTTCCTGAGGATTACTCAGTTAGTTGCATAAGATTAGTTCGGTTGTGGATCGCTGAAGGCTTTGTGAAACCGAAGATGGGCAAGACACTAGAAGAGGTTGGAGAGGAATACTTGATGGAGCTAATTCACAGAAGCCTGGTTCAAGTTTCAGAAGTTTACATTGATGGAAAAGCTCGAGGTTGTAGAGTTCATGATCTATTGCACGACGTCCTCCTTAAAAAGGGGTTGGATTCCAGCTTCTGTCATGTGTTTATCAAAAGGCAAGTTGACTTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0002376 GO:0003674 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0008219 GO:0009626 GO:0009987 GO:0012501 GO:0016020 GO:0019897 GO:0019898 GO:0023052 GO:0033554 GO:0034050 GO:0036094 GO:0044425 GO:0044459 GO:0044464 GO:0045087 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071944 GO:0097159 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko04626 map04626

Pfam Domains

Protein Families

NB-ARC

Protein Analysis

536

Amino Acids

61.47

Weight (kDa)

6.8

Isoelectric Point (pI)

41.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Rx_N	PF18052	5 - 99	4e-20	Rx N-terminal domain
NB-ARC	PF00931	182 - 356	2.8e-42	NB-ARC domain
WHD_DRP	PF23559	444 - 514	4.2e-25	Disease resistance protein Winged helix domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003960)

Species	Orthologous Gene IDs
malus_domestica	MD00G1097800.v1.1 MD01G1186500.v1.1 MD01G1186600.v1.1 MD01G1186800.v1.1 MD01G1187000.v1.1 MD01G1187100.v1.1 MD07G1258600.v1.1 MD07G1258700.v1.1 MD07G1258800.v1.1 MD07G1258900.v1.1 MD07G1259200.v1.1 MD07G1259300.v1.1
prunus_persica	Prupe.2G283200_v2.0.a1 Prupe.2G283300_v2.0.a1 Prupe.2G283400_v2.0.a1
pyrus_communis	pycom01g19810 pycom01g19820 pycom01g19840 pycom01g19900 pycom07g23170 pycom07g23230 pycom07g23240 pycom07g23250 pycom07g23260
rosa_chinensis	RchiOBHm_Chr1g0375241
rosa_laevigata	RLG00000026676
rosa_multiflora	Rmu_co8330553.1_g000001 Rmu_sc0000554.1_g000032
rosa_roxburghii	Rroxscaffold_4G00282910
rosa_rugosa	Rorug01G0389400
rosa_samantha	Rh1AG400100 Rh1BG364400 Rh1DG395000
rosa_wichuraiana	Rw1G035520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	475
AatII	GACGTC	1 cut(s)	1542
AciI	CCGC	2 cut(s)	265, 927
AclWI	GGATC	2 cut(s)	732, 1388
AcuI	CTGAAG	3 cut(s)	111, 1107, 1407
AcyI	GRCGYC	1 cut(s)	1539
AfaI	GTAC	3 cut(s)	88, 713, 851
AfiI	CCNNNNNNNGG	4 cut(s)	493, 661, 1409, 1552
AflII	CTTAAG	1 cut(s)	1219
AgsI	TTSAA	8 cut(s)	44, 239, 430, 536, 762, 956, 1067, 1475
AjiI	CACGTC	1 cut(s)	587
AjnI	CCWGG	1 cut(s)	1467
AluBI	AGCT	9 cut(s)	227, 518, 553, 1181, 1218, 1281, 1453, 1505, 1569
AluI	AGCT	9 cut(s)	227, 518, 553, 1181, 1218, 1281, 1453, 1505, 1569
Alw26I	GTCTC	3 cut(s)	170, 577, 1110
AlwI	GGATC	2 cut(s)	732, 1388
AlwNI	CAGNNNCTG	2 cut(s)	1015, 1572
Ama87I	CYCGRG	2 cut(s)	489, 1506
AoxI	GGCC	1 cut(s)	448
ApeKI	GCWGC	6 cut(s)	158, 161, 164, 224, 838, 1009
ArsI	GACNNNNNNTTYG	2 cut(s)	189, 221
AsuHPI	GGTGA	2 cut(s)	676, 1277
AsuII	TTCGAA	1 cut(s)	365
AvaI	CYCGRG	2 cut(s)	489, 1506
AxyI	CCTNAGG	1 cut(s)	1338
BbvCI	CCTCAGC	1 cut(s)	1277
BbvI	GCAGC	6 cut(s)	145, 173, 176, 236, 850, 1021
BccI	CCATC	7 cut(s)	164, 347, 661, 1296, 1402, 1441, 1490
BcgI	CGANNNNNNTGC	2 cut(s)	54, 88
BciT130I	CCWGG	1 cut(s)	1469
BclI	TGATCA	1 cut(s)	439
BcoDI	GTCTC	3 cut(s)	170, 577, 1110
BfaI	CTAG	1 cut(s)	1421
BfmI	CTRYAG	2 cut(s)	159, 754
BfrI	CTTAAG	1 cut(s)	1219
BglII	AGATCT	1 cut(s)	980
BisI	GCNGC	6 cut(s)	159, 162, 165, 225, 839, 1010
BlsI	GCNGC	6 cut(s)	160, 163, 166, 226, 840, 1011
Bme1390I	CCNGG	1 cut(s)	1469
BmeT110I	CYCGRG	2 cut(s)	489, 1506
BmgBI	CACGTC	1 cut(s)	587
BmrFI	CCNGG	1 cut(s)	1469
BmrI	ACTGGG	1 cut(s)	700
BmsI	GCATC	7 cut(s)	132, 139, 276, 286, 1021, 1115, 1338
BmuI	ACTGGG	1 cut(s)	700
BplI	GAGNNNNNCTC	2 cut(s)	1331, 1363
Bpu10I	CCTNAGC	1 cut(s)	1277
Bpu14I	TTCGAA	1 cut(s)	365
BpuEI	CTTGAG	1 cut(s)	122
BsaAI	YACGTR	1 cut(s)	86
BsaBI	GATNNNNATC	1 cut(s)	430
BsaHI	GRCGYC	1 cut(s)	1539
BsaJI	CCNNGG	1 cut(s)	291
BsaWI	WCCGGW	1 cut(s)	324
BsaXI	ACNNNNNCTCC	2 cut(s)	1064, 1094
Bsc4I	CCNNNNNNNGG	4 cut(s)	493, 661, 1409, 1552
Bse1I	ACTGG	1 cut(s)	706
Bse21I	CCTNAGG	1 cut(s)	1338
Bse8I	GATNNNNATC	1 cut(s)	430
BseBI	CCWGG	1 cut(s)	1469
BseDI	CCNNGG	1 cut(s)	291
BseGI	GGATG	3 cut(s)	123, 358, 805
BseJI	GATNNNNATC	1 cut(s)	430
BseLI	CCNNNNNNNGG	4 cut(s)	493, 661, 1409, 1552
BseMII	CTCAG	4 cut(s)	722, 1291, 1329, 1362
BseNI	ACTGG	1 cut(s)	706
BseRI	GAGGAG	1 cut(s)	818
BseXI	GCAGC	6 cut(s)	145, 173, 176, 236, 850, 1021
BshFI	GGCC	1 cut(s)	450
BsiHKCI	CYCGRG	2 cut(s)	489, 1506
BsiSI	CCGG	1 cut(s)	325
BslFI	GGGAC	2 cut(s)	688, 793
BslI	CCNNNNNNNGG	4 cut(s)	493, 661, 1409, 1552
BsmAI	GTCTC	3 cut(s)	170, 577, 1110
BsmBI	CGTCTC	2 cut(s)	170, 577
BsmFI	GGGAC	2 cut(s)	688, 793
BsnI	GGCC	1 cut(s)	450
BsoBI	CYCGRG	2 cut(s)	489, 1506
Bsp119I	TTCGAA	1 cut(s)	365
Bsp143I	GATC	8 cut(s)	27, 128, 344, 439, 724, 980, 1380, 1525
Bsp19I	CCATGG	1 cut(s)	291
BspACI	CCGC	2 cut(s)	265, 927
BspANI	GGCC	1 cut(s)	450
BspCNI	CTCAG	4 cut(s)	723, 1290, 1330, 1361
BspHI	TCATGA	1 cut(s)	1522
BspMAI	CTGCAG	1 cut(s)	163
BspPI	GGATC	2 cut(s)	732, 1388
BspT104I	TTCGAA	1 cut(s)	365
BspTI	CTTAAG	1 cut(s)	1219
BsrI	ACTGG	1 cut(s)	706
BssECI	CCNNGG	1 cut(s)	291
BssMI	GATC	8 cut(s)	27, 128, 344, 439, 724, 980, 1380, 1525
BssNI	GRCGYC	1 cut(s)	1539
BssT1I	CCWWGG	1 cut(s)	291
Bst2UI	CCWGG	1 cut(s)	1469
Bst4CI	ACNGT	7 cut(s)	191, 377, 477, 700, 739, 758, 1296
Bst6I	CTCTTC	3 cut(s)	1083, 1092, 1419
BstACI	GRCGYC	1 cut(s)	1539
BstAFI	CTTAAG	1 cut(s)	1219
BstAPI	GCANNNNNTGC	1 cut(s)	1015
BstBAI	YACGTR	1 cut(s)	86
BstBI	TTCGAA	1 cut(s)	365
BstDEI	CTNAG	4 cut(s)	731, 1277, 1338, 1348
BstDSI	CCRYGG	1 cut(s)	291
BstENI	CCTNNNNNAGG	1 cut(s)	1550
BstF5I	GGATG	3 cut(s)	123, 358, 805
BstKTI	GATC	8 cut(s)	30, 131, 347, 442, 727, 983, 1383, 1528
BstMAI	GTCTC	3 cut(s)	170, 577, 1110
BstMBI	GATC	8 cut(s)	27, 128, 344, 439, 724, 980, 1380, 1525
BstMWI	GCNNNNNNNGC	4 cut(s)	62, 158, 164, 1015
BstNI	CCWGG	1 cut(s)	1469
BstNSI	RCATGY	1 cut(s)	908
BstSCI	CCNGG	1 cut(s)	1467
BstSFI	CTRYAG	2 cut(s)	159, 754
BstV1I	GCAGC	6 cut(s)	145, 173, 176, 236, 850, 1021
BstX2I	RGATCY	2 cut(s)	724, 980
BstXI	CCANNNNNNTGG	1 cut(s)	458
BstYI	RGATCY	2 cut(s)	724, 980
Bsu36I	CCTNAGG	1 cut(s)	1338
BsuRI	GGCC	1 cut(s)	450
BtgI	CCRYGG	1 cut(s)	291
BtgZI	GCGATG	1 cut(s)	37
BtrI	CACGTC	1 cut(s)	587
BtsCI	GGATG	3 cut(s)	123, 358, 805
BtsI	GCAGTG	1 cut(s)	463
BtsIMutI	CAGTG	3 cut(s)	463, 606, 705
CaiI	CAGNNNCTG	2 cut(s)	1015, 1572
CciI	TCATGA	1 cut(s)	1522
Csp6I	GTAC	3 cut(s)	87, 712, 850
CviAII	CATG	7 cut(s)	292, 610, 905, 995, 1032, 1523, 1577
CviQI	GTAC	3 cut(s)	87, 712, 850
DdeI	CTNAG	4 cut(s)	731, 1277, 1338, 1348
DpnI	GATC	8 cut(s)	29, 130, 346, 441, 726, 982, 1382, 1527
DpnII	GATC	8 cut(s)	27, 128, 344, 439, 724, 980, 1380, 1525
DrdI	GACNNNNNNGTC	1 cut(s)	475
DseDI	GACNNNNNNGTC	1 cut(s)	475
Eam1104I	CTCTTC	3 cut(s)	1083, 1092, 1419
EarI	CTCTTC	3 cut(s)	1083, 1092, 1419
EciI	GGCGGA	1 cut(s)	942
Eco130I	CCWWGG	1 cut(s)	291
Eco32I	GATATC	1 cut(s)	597
Eco57I	CTGAAG	3 cut(s)	111, 1107, 1407
Eco81I	CCTNAGG	1 cut(s)	1338
Eco88I	CYCGRG	2 cut(s)	489, 1506
EcoNI	CCTNNNNNAGG	1 cut(s)	1550
EcoRII	CCWGG	1 cut(s)	1467
EcoRV	GATATC	1 cut(s)	597
EcoT14I	CCWWGG	1 cut(s)	291
ErhI	CCWWGG	1 cut(s)	291
Esp3I	CGTCTC	2 cut(s)	170, 577
FaeI	CATG	7 cut(s)	295, 613, 908, 998, 1035, 1526, 1580
FalI	AAGNNNNNCTT	2 cut(s)	54, 86
FaqI	GGGAC	2 cut(s)	688, 793
FatI	CATG	7 cut(s)	291, 609, 904, 994, 1031, 1522, 1576
FbaI	TGATCA	1 cut(s)	439
Fnu4HI	GCNGC	6 cut(s)	159, 162, 165, 225, 839, 1010
FokI	GGATG	3 cut(s)	110, 365, 812
Fsp4HI	GCNGC	6 cut(s)	159, 162, 165, 225, 839, 1010
FspBI	CTAG	1 cut(s)	1421
GluI	GCNGC	6 cut(s)	159, 162, 165, 225, 839, 1010
HaeIII	GGCC	1 cut(s)	450
HapII	CCGG	1 cut(s)	325
Hin1I	GRCGYC	1 cut(s)	1539
Hin1II	CATG	7 cut(s)	295, 613, 908, 998, 1035, 1526, 1580
HincII	GTYRAC	3 cut(s)	373, 1173, 1600
HindII	GTYRAC	3 cut(s)	373, 1173, 1600
HinfI	GANTC	8 cut(s)	296, 426, 469, 536, 806, 952, 1266, 1562
HpaII	CCGG	1 cut(s)	325
HphI	GGTGA	2 cut(s)	676, 1277
Hpy166II	GTNNAC	4 cut(s)	373, 1173, 1489, 1600
Hpy188I	TCNGA	5 cut(s)	128, 732, 1195, 1228, 1483
Hpy188III	TCNNGA	7 cut(s)	75, 395, 825, 956, 1085, 1337, 1523
Hpy8I	GTNNAC	4 cut(s)	373, 1173, 1489, 1600
Hpy99I	CGWCG	2 cut(s)	583, 1541
HpyAV	CCTTC	8 cut(s)	310, 566, 655, 760, 917, 1045, 1124, 1382
HpyCH4III	ACNGT	7 cut(s)	191, 377, 477, 700, 739, 758, 1296
HpyCH4IV	ACGT	3 cut(s)	85, 586, 1539
HpyCH4V	TGCA	7 cut(s)	83, 161, 838, 975, 1009, 1359, 1534
HpyF10VI	GCNNNNNNNGC	4 cut(s)	62, 158, 164, 1015
HpyF3I	CTNAG	4 cut(s)	731, 1277, 1338, 1348
HpySE526I	ACGT	3 cut(s)	85, 586, 1539
Hsp92I	GRCGYC	1 cut(s)	1539
Hsp92II	CATG	7 cut(s)	295, 613, 908, 998, 1035, 1526, 1580
Ksp22I	TGATCA	1 cut(s)	439
Kzo9I	GATC	8 cut(s)	27, 128, 344, 439, 724, 980, 1380, 1525
LmnI	GCTCC	4 cut(s)	267, 575, 769, 1450
Lsp1109I	GCAGC	6 cut(s)	145, 173, 176, 236, 850, 1021
LweI	GCATC	7 cut(s)	132, 139, 276, 286, 1021, 1115, 1338
MaeI	CTAG	1 cut(s)	1421
MaeII	ACGT	3 cut(s)	85, 586, 1539
MalI	GATC	8 cut(s)	29, 130, 346, 441, 726, 982, 1382, 1527
MboI	GATC	8 cut(s)	27, 128, 344, 439, 724, 980, 1380, 1525
MboII	GAAGA	6 cut(s)	251, 719, 1100, 1109, 1417, 1436
MfeI	CAATTG	1 cut(s)	1146
MflI	RGATCY	2 cut(s)	724, 980
MlyI	GAGTC	2 cut(s)	463, 815
MmeI	TCCRAC	3 cut(s)	1074, 1411, 1539
MseI	TTAA	6 cut(s)	38, 510, 1110, 1220, 1548, 1606
MslI	CAYNNNNRTG	1 cut(s)	857
MspCI	CTTAAG	1 cut(s)	1219
MspI	CCGG	1 cut(s)	325
MspR9I	CCNGG	1 cut(s)	1469
MunI	CAATTG	1 cut(s)	1146
MvaI	CCWGG	1 cut(s)	1469
MwoI	GCNNNNNNNGC	4 cut(s)	62, 158, 164, 1015
NcoI	CCATGG	1 cut(s)	291
NdeII	GATC	8 cut(s)	27, 128, 344, 439, 724, 980, 1380, 1525
NlaIII	CATG	7 cut(s)	295, 613, 908, 998, 1035, 1526, 1580
NspI	RCATGY	1 cut(s)	908
NspV	TTCGAA	1 cut(s)	365
PaeR7I	CTCGAG	2 cut(s)	489, 1506
PagI	TCATGA	1 cut(s)	1522
PfeI	GAWTC	6 cut(s)	296, 426, 536, 952, 1266, 1562
PflFI	GACNNNGTC	1 cut(s)	191
PkrI	GCNGC	6 cut(s)	160, 163, 166, 226, 840, 1011
PleI	GAGTC	2 cut(s)	463, 814
PpsI	GAGTC	2 cut(s)	463, 814
Ppu21I	YACGTR	1 cut(s)	86
Psp6I	CCWGG	1 cut(s)	1467
PspGI	CCWGG	1 cut(s)	1467
PspXI	VCTCGAGB	2 cut(s)	489, 1506
PsrI	GAACNNNNNNTAC	2 cut(s)	894, 926
PstI	CTGCAG	1 cut(s)	163
PstNI	CAGNNNCTG	2 cut(s)	1015, 1572
PsuI	RGATCY	2 cut(s)	724, 980
PsyI	GACNNNGTC	1 cut(s)	191
RsaI	GTAC	3 cut(s)	88, 713, 851
RsaNI	GTAC	3 cut(s)	87, 712, 850
RseI	CAYNNNNRTG	1 cut(s)	857
SaqAI	TTAA	6 cut(s)	38, 510, 1110, 1220, 1548, 1606
SatI	GCNGC	6 cut(s)	159, 162, 165, 225, 839, 1010
Sau3AI	GATC	8 cut(s)	27, 128, 344, 439, 724, 980, 1380, 1525
SchI	GAGTC	2 cut(s)	463, 815
ScrFI	CCNGG	1 cut(s)	1469
SfaNI	GCATC	7 cut(s)	132, 139, 276, 286, 1021, 1115, 1338
SfcI	CTRYAG	2 cut(s)	159, 754
Sfr274I	CTCGAG	2 cut(s)	489, 1506
SfuI	TTCGAA	1 cut(s)	365
SlaI	CTCGAG	2 cut(s)	489, 1506
SmiMI	CAYNNNNRTG	1 cut(s)	857
SmlI	CTYRAG	4 cut(s)	137, 489, 1219, 1506
SmoI	CTYRAG	4 cut(s)	137, 489, 1219, 1506
SsiI	CCGC	2 cut(s)	265, 927
SspI	AATATT	1 cut(s)	257
SspMI	CTAG	1 cut(s)	1421
StyD4I	CCNGG	1 cut(s)	1467
StyI	CCWWGG	1 cut(s)	291
TaaI	ACNGT	7 cut(s)	191, 377, 477, 700, 739, 758, 1296
TaiI	ACGT	3 cut(s)	88, 589, 1542
TaqI	TCGA	8 cut(s)	30, 74, 117, 365, 479, 490, 824, 1507
TfiI	GAWTC	6 cut(s)	296, 426, 536, 952, 1266, 1562
Tru1I	TTAA	6 cut(s)	38, 510, 1110, 1220, 1548, 1606
Tru9I	TTAA	6 cut(s)	38, 510, 1110, 1220, 1548, 1606
TscAI	CASTG	3 cut(s)	463, 606, 705
TseI	GCWGC	6 cut(s)	158, 161, 164, 224, 838, 1009
TspDTI	ATGAA	8 cut(s)	91, 126, 267, 496, 936, 983, 989, 1511
TspGWI	ACGGA	2 cut(s)	964, 1144
TspRI	CASTG	3 cut(s)	463, 606, 705
Tth111I	GACNNNGTC	1 cut(s)	191
Vha464I	CTTAAG	1 cut(s)	1219
XagI	CCTNNNNNAGG	1 cut(s)	1550
XceI	RCATGY	1 cut(s)	908
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1029
XhoI	CTCGAG	2 cut(s)	489, 1506
XspI	CTAG	1 cut(s)	1421
ZraI	GACGTC	1 cut(s)	1540

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.