MD07G1259300.v1.1

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0003960
Physical Location & Seq
Forward (+)
32556073 .. 32563190
7118 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1259300.v1.1.491

Sequence Viewer

Length: 5613 bp
ATGGCAGAACAAATTGTATGCTTTGTGATCGACAAGTTGATTCAACTACTCACCACCACAGAGGCAAACCTTTCAAGAGAAGTACGCAAAGAAGTTAGTTTCATCAGAGGTGAGCTACAGACCATCGGATCATTCCTCAAGGACGCAGATGCTAAGGCGGCAGCAGATGGAGAGAGGACGAATGACTGTGTCAAAACTTGGGTCAATCAAGTAAGAGAAGCAGCTTTTTGCATTGAAGATGTTATTGATGAATATTTGCTCCGTATCACACGGCATCGTCAACACCATAGATTCATTCGTTTTTTCCATAAAATTATCCGGTTGGTTAAAAAAATGAACTTACCCGATGGGATAGTTTCAGAAGTTGACGATATAAAACGATTGGTTCATGACATAAAGGAGAGAAGTGTAAGATACGAATTCAGTTCCACAGACCAGCAAGGTCAAGATAATGGCGAAAAGACTCTTCCGTGGCATGACCCTCGAGTGGATTCCCTTTTCATTGAGGAAGCTGAAGTTGTGGGGATTGAGTCTGACAGAGAAAAGCTAACTGATTTGTTGGTAGGAGGAACGCCGAGACGTGAAGTGATATCAGTGGTTGGCATGGGAGGACTTGGGAAAACCACTCTTGCCAAGAAAGTTTATGACAATCAGACGGAGATGGCGCACTTTGATTGCTACGCTTGGATTACGGTGTCCCAGACATACAGGGTGGAGGATCTTCTAAGGACTGTAATCAAAATGTTTTATAATTCAAGGAAGGAGAATTTCCCAGAGGAAATTGAAACAATGGATGCGGAGTCCCTGATCAGAAAATCAAGAGAGTATTTGCAGCAAAAGAGGTATGTTGTTGTGTTTGATGATGTGTGGAAAATAGATTTTTGGGGGGCTATAGAACATGCTTTACCGGATAATAAAGGGGGACGAATAATGATTACTACAAGGATTCAAGATGTTGCCGATTTTTGCAAAAAATCTTGTTTTGTTCATGTCCATCACTTGCAACCTCTGTCTCCAAACAAGGCATGGGAATTGTTTTGTAGAAAGGCATTTCAGTTTGAATTGGAGGGAAATTGTCCTCCAGAGTTAAAAGATATGTCACAGGAAATCGTTAGAAAGTGTGAAGGACTGCCTTTGGCAATTGTTTGCATAGGTGGTCTGCTGTCAACGAAAGTTAAAGTGGTGTCTGAATGGCAGAAGTTATACAATAGCTTAAGTTCAGAGTTGGAAAGCAATCCCCATCTTACAAGCCTCACAAGAATCATATCCCTCAGCTATCACCACCTACCATATTACCTCAAATCATGCCTCTTGTACTTTGGCATCTTTCCAGAGGACTACTCAATTAATTGCGTAAGATTGATTCGGTTGTGGATTGCTGAAGGGTTTGTAAAATTGAAGAAGGGCAAGACACTGGAAGAGGTTGGAGAGGAATACTTGACTGAGCTAATTCACAGAAGCCTGGTTCAAGTGTCAGAAGTTTACATTGATGGAAAAGCTCGAAGTTGTAGAGTTCATGATCTTTTGCTAGAAGTGCTCCTTAAAAAGGGTGTGGATTCAAGCTTCTGTCATGTGCTATCAGAATTTGAGTTCACTTCTGAATCAACAATTCGACGTCTCTCAATCGACAGCAGTCCATCTGATGCCTTGCGAAGCATTAGAGAGTCTCAAATTCGCTCTGTATTTACTTTCAACCAAGAAGAATGGCCCGAGTCTTTTCTCATTACGTTGAGTGGAAACTTCAAACTTCTGAAAGTATTGGATTTCATGGATGCTCCAATCAATCAACTTCCGAAATATATGGGGGATTTGTATCTCTTGACGTACTTAAGCCTGAGGAATACAAAGGTGGAGTTGCTTCCAGACTCCATAGGCAATCTGCTAAATTTAGAAACGCTGGATCTAAAACAGTCTTTGGTGTATGATATATTGAGAGACCTAACCCAGTCACAATTTAGTTACAACAAGAACTTTGAGCAGTGGTTTGAAAAAGACCAAAACCTCTTGATTTGGCTCAACTCGACTTTATCCGAAGATCTCATTCCCTTCACGGTAGGGGTGTCCACATCTCGAGAACTTTGGCAGAAGCTTGAACAACGCTTTGGTGGCGTCTCTGAAGCTCACATTCACCAGCTTCGTTCTCGTCTTCAATTGATTCAAAAAGGATCTCAATCGATCTCGGAATATCTACAGCAAATCAAGGAAATTTCGGATTCTCTCAATGCTGCTGGCGCCACTGTATCTGATCGTGATCTCATTGCCGCAACGCTTCACGGTCTTCCTGATGAATTTGAGTCCTTTATTGACTCCATCATGCTGCGGTTGTCTTCTACTTCTCTTGATGAATTGCATGGTCTGTTGCTGACCAAAGAGCTTTCCCTGGCTCGTCGCAAGCACACTCTGTCTCCCACTGCTACGGAGCCCTTTCAAGCTTTCTCTGTTCAGTCTCAAGCACCTTTGCTCCCAACGCCTTTTTCGCAGGCTTTTGCTGCATATAGTCAGCCTCTTCAATCTTCTTCTCGGATGAATCCTGAGATCTTTGGCCGGGTGCCACCAGCCAAACTTGCTGCTCTTTGTGCTCACTACTCATCCAAACCATCCCCATCTTGGCTATTGGATTCTGGAGCCACCTCACATATCACGAATGACATTGCAAATCTCTCTGTTGCCTCTCCTTACACTGGTGAAGACAAGGTCTATATAGGAGATGGTAAAGGTCTGCCTATTCACAATATTGGTATCTCTTCTTTAGATACATCTCATACTTCCTTCAAGTTGCGTAATGTCTTACATGTACCTGCTATGAAACACAACTTATTATCTGCCTATCAATTTTTAAAAGATAATAATTGTGCCTTGACTCTTGACCCTGATGGATCCACTGTAAAGGATCGAGTTTCGGGGAAGACGCTTTTGCGGGGACGAGTTGAAGATGGCTTATATCCTCTTCAAGGCTCTAGTCATCCCACCTCCTCATCACCCTCGGCTTTAATAAGTGTCAAAGCTCCTGTCCAAATTTGGCACCGAAGATTGGGTCATCCATCTTCCTCTATTTTTCGCAAGGTTCTTTCAGGAAATAAGCTTGTCGTGCAAGGCAAAACCTGCTGTTTACAGCACAAAGGTTATAGATGCCTTGATCCAGTCACTAATCGTCTCTACATTTCCAGACATGTGCAGTTTGATGAAGCCACATATCCTTTTCAGTCTTCTCCTACCATCCCTGGTCTGTCTTCAGACTCTGTTACCGCTCCCATTGATCTGCAGTTCTCCGTTTCTCAACATCCTGGTGTTCTTCCTGGCCCTTTGGTTCCAGATTCAAGTCTACACGTTGGCCATACTCCAGAGTCCATTTCTCCTACTGCTGCTCCAAGTACAGATTCTCTGAATAATGCATCTGTATCTTCTCCTGCAACAACTATGTCCCTGCCCCCTTCCAATACTCACCCCATGCTCACTCGCTCCAAAGCTGGTATCTTCAAGCCCAAAGTTTATGCAGCTACCAAACATCCTCTTCCATCTGCTATTGACTTTATTCCATCTACATATCTGCAAGCATCCAAACATGCTCATTGGAGATCTGCCATGCAAGATGAGTTTAATGCATTACAATCAACAGGCACCTGGACCCTTGTTCCTCCATCTTCCTCCTACAATGTGGTTGGCTGCAAGTGGGTGTTCCGAATCAAGAAGAAACCTGATGGTTCTGTTGAGCGCTTTAAAGCTCGTTTAGTTGCAAAGGGGTATCATCAACAGGAAGGTATCGATTTTCAAGAGACCTTCAGTCCTGTGGCTAAACCAGTCACCATTAGAATCCTTTTGTCTCTTGCTGTGCAGTTCAATTGGTTTCTAAATCAGTTAGACATCAGTAATGCCTTTCTTCATGGTGATTTGAAGGAAGATGTGTACATGCAGCAGCCTCCCGGTTTCTCTGATCCTTCTATGCCGCATCATGTCTGCAAATTAAGGAAATCCCTTTATGGTCTCAAACAAGCCCCTCGGGCTTGGTTTGACAAACTGTTTCAAGCTCTTCTTCAGCTTGGTTTTCAGCAATCTTCCTCAGATGCTTCCTTATTTGTTCTCCCTGGTCCTACTCCTGTCATGGTGCTTGTGTATGTAGATGATATATTAGTCACTGGACCTGACTCTTCCTTATGCAGTCTCTTCATTCAAAATCTCGGTACTATTTTTCCAGTCAAGGATTTGGGTCCACTACACTACTTCCTGGGTTTAGAAATTCATAGATCTTCCACAGGTCTCTTCCTCCATCAAACAAAGTATTTACTTGATCTTCTCCGGAAAACTAATATGGCTGGTGCGAAGCCTTGCTGCACTCCCCTTGGCTCTAACAAATTGGATCACAGTGGTCCTCTTTTCTCTCATCCCACTGAGTATCGTTCCATTGTTGGTGGTTTACATTTGACATGGACACGTCCAGATATTTCGTTTGCTGTGAATCAAATCTGTCAGTTCATGCATGCTCCTCGAGACCAACACATGCAAGCTGCCAAACGGATTCTTCGTTATCTCAAGGGTACTGTCTCTGAAGGTCTATGGTTCAGAAAGGGTACCACTAATCTCACAGCCTTCTCCGATGCAGACTGGGCTGGCTGTCTCTTTGATCGCCGTTCTACTAGTGGTTACAATGTCTTTCTCGGATCCAATCTTATCAGTTGGAGTGCCAAGAAACAGGCTACTGTGGCACGGTCCTCCACAGAGGCGGAATACAGATCTCTAGCCCACACTGCAGCTGAACTGACTTGGATCTGTAAAAATTTTCGTGACCTTGGTTTTCCTCTCTCTCAGGCTCCCACTCTCTGGTGTGATAACATTTCAGCCATCTCTCTTGCTTCTAATCCGGTCTTTCATGCCCGAACTAAACATGTGGAGATTGACTATCATTATATTCGTGAGCCAAGTGGAGGAATCAAACTAATCAAAGAACTGGGAATGTTAAGGCAGCTGCGGAAGTTAGGCCTAAACAACTTGAAAGAGGAAGATGGAAGGGCTCTGTGCGCTTCTATTGACAAGATGAATCACCTTGAATGTCTAGATTTAACTGCTACAAGTGAAGATGAAGTCCTTGATCTAGAATCCATTTCAACTCCACCAGAATTTATTCGGTCTCTGTACTTGAGGGGGCGTTTAGAGCAGTTGCCAAGTTGGATTTCAAATCTTCAACACCTCGTCAAATTGGGGATTTATCTGTCAAGGTTGAGAGATTCCCCCTTGAAAGCCCTTCAAAATCTACCTAATTTGTTGGATCTTCGTTTGGGACAAATGGGATATGATGGCGGGCAGCTGCATTTTGAGGGAGGAGGATTTCAGAAACTTAGGGAGCTAAAACTCGGGGAAATGGAGGGACTAAATTCACTGATCATAGACAATGGGGTTATGCCTCTTCTCCAAGAGTTCCACATTGGACCCTGCCCACAACTGAAGGAGGTGCCATCTGGCCTCCACCATCTCCGAAATCTGAGATATCTTTGGATTTATGGCATGCCGAAGGAGTTTGCACGACAAATGGATCCAAATAATGACCCACAGTATTGGATTGTTAGACATATACGACATGTTGTTTTTAGTTACATGTCTGGCCTGAGATTAGGCGAATAA

Protein Analysis

1871

Amino Acids

210.94

Weight (kDa)

7.6

Isoelectric Point (pI)

47.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 5 - 103 2.9e-22 Rx N-terminal domain
NB-ARC PF00931 178 - 354 3.4e-42 NB-ARC domain
WHD_DRP PF23559 442 - 511 5.9e-24 Disease resistance protein Winged helix domain
LRR_14 PF23598 557 - 638 1e-10 Leucine-rich repeat region
Retrotran_gag_2 PF14223 656 - 792 1.4e-15 gag-polypeptide of LTR copia-type
Pol_BBD PF22936 870 - 947 4.3e-14 Pol polyprotein, beta-barrel domain
gag_pre-integrs PF13976 979 - 1027 1e-07 GAG-pre-integrase domain
SH3_retrovirus PF25597 1037 - 1071 1.6e-09 Retroviral polymerase SH3-like domain
RVT_2 PF07727 1207 - 1446 1.6e-66 Reverse transcriptase (RNA-dependent DNA polymerase)
LRR_14 PF23598 1642 - 1830 1.9e-23 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 750
AatII GACGTC 1 cut(s) 1618
Acc36I ACCTGC 2 cut(s) 2806, 3110
Acc65I GGTACC 1 cut(s) 4565
AccB1I GGYRCC 6 cut(s) 2231, 2548, 3021, 3617, 4565, 5443
AccB7I CCANNNNNTGG 1 cut(s) 4815
AccBSI CCGCTC 1 cut(s) 3248
AccI GTMKAC 1 cut(s) 3322
AccIII TCCGGA 1 cut(s) 4293
AcoI YGGCCR 2 cut(s) 2542, 3331
AcuI CTGAAG 8 cut(s) 534, 1401, 2136, 3216, 3763, 4016, 4563, 5456
AcyI GRCGYC 3 cut(s) 1615, 2109, 2232
AfeI AGCGCT 1 cut(s) 3713
AfiI CCNNNNNNNGG 8 cut(s) 487, 1546, 2607, 2681, 2951, 3031, 4815, 4919
AflII CTTAAG 2 cut(s) 1213, 1828
AflIII ACRYGT 7 cut(s) 2791, 3169, 3325, 4427, 4879, 5569, 5586
AhdI GACNNNNNGTC 1 cut(s) 3780
AhlI ACTAGT 1 cut(s) 4631
AjiI CACGTC 2 cut(s) 581, 4430
AjnI CCWGG 8 cut(s) 1461, 2379, 3220, 3283, 3295, 3620, 4081, 4221
AjuI GAANNNNNNNTTGG 8 cut(s) 2085, 2117, 2458, 2490, 3361, 3393, 5149, 5181
Alw21I GWGCWC 2 cut(s) 1539, 2581
AlwNI CAGNNNCTG 1 cut(s) 3239
Ama87I CYCGRG 6 cut(s) 483, 1709, 2070, 3996, 4482, 5345
Aor13HI TCCGGA 1 cut(s) 4293
Aor51HI AGCGCT 1 cut(s) 3713
AoxI GGCC 7 cut(s) 1706, 2542, 3298, 3331, 4972, 5452, 5593
ArsI GACNNNNNNTTYG 6 cut(s) 186, 218, 2994, 3019, 3026, 3051
AseI ATTAAT 1 cut(s) 1347
Asp700I GAANNNNTTC 1 cut(s) 5115
Asp718I GGTACC 1 cut(s) 4565
AspLEI GCGC 4 cut(s) 667, 2234, 3714, 5015
AspS9I GGNCC 9 cut(s) 1707, 3299, 3624, 4085, 4136, 4205, 4364, 4704, 5420
AsuC2I CCSGG 2 cut(s) 2546, 3921
AvaI CYCGRG 6 cut(s) 483, 1709, 2070, 3996, 4482, 5345
AvaII GGWCC 7 cut(s) 3624, 4085, 4136, 4205, 4364, 4704, 5420
AxyI CCTNAGG 1 cut(s) 1835
BalI TGGCCA 1 cut(s) 3333
BamHI GGATCC 3 cut(s) 2876, 4655, 5524
BanI GGYRCC 6 cut(s) 2231, 2548, 3021, 3617, 4565, 5443
BanII GRGCYC 2 cut(s) 2424, 5008
BbsI GAAGAC 7 cut(s) 2138, 2270, 2319, 2694, 2912, 3198, 3222
Bbv12I GWGCWC 2 cut(s) 1539, 2581
BbvCI CCTCAGC 1 cut(s) 1271
BceAI ACGGC 2 cut(s) 287, 4608
BcgI CGANNNNNNTGC 4 cut(s) 464, 498, 4463, 4497
BciT130I CCWGG 8 cut(s) 1463, 2381, 3222, 3285, 3297, 3622, 4083, 4223
BclI TGATCA 2 cut(s) 807, 5373
BcnI CCSGG 2 cut(s) 2546, 3921
BcuI ACTAGT 1 cut(s) 4631
BfaI CTAG 6 cut(s) 1529, 2958, 4632, 4733, 5048, 5087
BfmI CTRYAG 5 cut(s) 116, 891, 2189, 3260, 4743
BfoI RGCGCY 2 cut(s) 2235, 3715
BfrI CTTAAG 2 cut(s) 1213, 1828
BfuAI ACCTGC 2 cut(s) 2806, 3110
BglI GCCNNNNNGGC 1 cut(s) 3998
BglII AGATCT 5 cut(s) 2035, 2535, 3575, 4241, 4727
Bme18I GGWCC 7 cut(s) 3624, 4085, 4136, 4205, 4364, 4704, 5420
BmeRI GACNNNNNGTC 1 cut(s) 3780
BmeT110I CYCGRG 6 cut(s) 483, 1709, 2070, 3996, 4482, 5345
BmgBI CACGTC 2 cut(s) 581, 4430
BmgT120I GGNCC 9 cut(s) 1707, 3299, 3624, 4085, 4136, 4205, 4364, 4704, 5420
BmrI ACTGGG 3 cut(s) 1939, 4609, 4952
BmuI ACTGGG 3 cut(s) 1939, 4609, 4952
BoxI GACNNNNGTC 1 cut(s) 1632
BpiI GAAGAC 7 cut(s) 2138, 2270, 2319, 2694, 2912, 3198, 3222
BplI GAGNNNNNCTC 2 cut(s) 1325, 1357
BpmI CTGGAG 3 cut(s) 1065, 2643, 3324
Bpu10I CCTNAGC 2 cut(s) 153, 1271
BpuEI CTTGAG 4 cut(s) 122, 2433, 4511, 5152
BpuMI CCSGG 2 cut(s) 2546, 3921
Bsa29I ATCGAT 2 cut(s) 2174, 3762
BsaBI GATNNNNATC 3 cut(s) 1812, 2170, 2250
BsaHI GRCGYC 3 cut(s) 1615, 2109, 2232
BsaI GGTCTC 6 cut(s) 1929, 3767, 3986, 4259, 4479, 5127
BsaJI CCNNGG 9 cut(s) 470, 2379, 2982, 3220, 3995, 4081, 4222, 4336, 4783
BsaWI WCCGGW 4 cut(s) 318, 907, 4293, 4855
BsaXI ACNNNNNCTCC 6 cut(s) 2389, 2419, 2445, 2475, 3349, 3379
Bsc4I CCNNNNNNNGG 8 cut(s) 487, 1546, 2607, 2681, 2951, 3031, 4815, 4919
Bse1I ACTGG 9 cut(s) 1419, 1945, 2686, 3140, 3797, 4138, 4190, 4604, 4947
Bse21I CCTNAGG 1 cut(s) 1835
Bse3DI GCAATG 2 cut(s) 2256, 2649
Bse8I GATNNNNATC 3 cut(s) 1812, 2170, 2250
BseAI TCCGGA 1 cut(s) 4293
BseBI CCWGG 8 cut(s) 1463, 2381, 3222, 3285, 3297, 3622, 4083, 4223
BseCI ATCGAT 2 cut(s) 2174, 3762
BseDI CCNNGG 9 cut(s) 470, 2379, 2982, 3220, 3995, 4081, 4222, 4336, 4783
BseJI GATNNNNATC 3 cut(s) 1812, 2170, 2250
BseLI CCNNNNNNNGG 8 cut(s) 487, 1546, 2607, 2681, 2951, 3031, 4815, 4919
BseMI GCAATG 2 cut(s) 2256, 2649
BseMII CTCAG 9 cut(s) 1285, 1434, 1826, 2523, 4071, 4377, 4814, 5465, 5588
BseNI ACTGG 9 cut(s) 1419, 1945, 2686, 3140, 3797, 4138, 4190, 4604, 4947
BseRI GAGGAG 3 cut(s) 2962, 4470, 5328
BsgI GTGCAG 3 cut(s) 3194, 3851, 4312
BshFI GGCC 7 cut(s) 1708, 2544, 3300, 3333, 4974, 5454, 5595
BshNI GGYRCC 6 cut(s) 2231, 2548, 3021, 3617, 4565, 5443
BshVI ATCGAT 2 cut(s) 2174, 3762
BsiHKAI GWGCWC 2 cut(s) 1539, 2581
BsiHKCI CYCGRG 6 cut(s) 483, 1709, 2070, 3996, 4482, 5345
BsiSI CCGG 6 cut(s) 319, 908, 2545, 3921, 4294, 4856
BslFI GGGAC 7 cut(s) 682, 787, 936, 2934, 3406, 5286, 5373
BslI CCNNNNNNNGG 8 cut(s) 487, 1546, 2607, 2681, 2951, 3031, 4815, 4919
BsmBI CGTCTC 4 cut(s) 571, 1622, 2116, 3158
BsmFI GGGAC 7 cut(s) 682, 787, 936, 2934, 3406, 5286, 5373
BsnI GGCC 7 cut(s) 1708, 2544, 3300, 3333, 4974, 5454, 5595
Bso31I GGTCTC 6 cut(s) 1929, 3767, 3986, 4259, 4479, 5127
BsoBI CYCGRG 6 cut(s) 483, 1709, 2070, 3996, 4482, 5345
Bsp1286I GDGCHC 4 cut(s) 1539, 2424, 2581, 5008
Bsp13I TCCGGA 1 cut(s) 4293
Bsp1407I TGTACA 1 cut(s) 3903
BspANI GGCC 7 cut(s) 1708, 2544, 3300, 3333, 4974, 5454, 5595
BspCNI CTCAG 9 cut(s) 1284, 1435, 1827, 2524, 4070, 4378, 4813, 5466, 5589
BspDI ATCGAT 2 cut(s) 2174, 3762
BspEI TCCGGA 1 cut(s) 4293
BspHI TCATGA 2 cut(s) 388, 1516
BspMAI CTGCAG 2 cut(s) 3264, 4747
BspMI ACCTGC 2 cut(s) 2806, 3110
BspQI GCTCTTC 1 cut(s) 4032
BspT107I GGYRCC 6 cut(s) 2231, 2548, 3021, 3617, 4565, 5443
BspTI CTTAAG 2 cut(s) 1213, 1828
BspTNI GGTCTC 6 cut(s) 1929, 3767, 3986, 4259, 4479, 5127
BsrBI CCGCTC 1 cut(s) 3248
BsrDI GCAATG 2 cut(s) 2256, 2649
BsrGI TGTACA 1 cut(s) 3903
BsrI ACTGG 9 cut(s) 1419, 1945, 2686, 3140, 3797, 4138, 4190, 4604, 4947
BssECI CCNNGG 9 cut(s) 470, 2379, 2982, 3220, 3995, 4081, 4222, 4336, 4783
BssNI GRCGYC 3 cut(s) 1615, 2109, 2232
BssT1I CCWWGG 2 cut(s) 4336, 4783
Bst2UI CCWGG 8 cut(s) 1463, 2381, 3222, 3285, 3297, 3622, 4083, 4223
BstACI GRCGYC 3 cut(s) 1615, 2109, 2232
BstAFI CTTAAG 2 cut(s) 1213, 1828
BstAPI GCANNNNNTGC 1 cut(s) 3102
BstAUI TGTACA 1 cut(s) 3903
BstC8I GCNNGC 9 cut(s) 2230, 2393, 2481, 3552, 4476, 4500, 4606, 5294, 5498
BstDSI CCRYGG 1 cut(s) 470
BstENI CCTNNNNNAGG 2 cut(s) 1544, 2949
BstH2I RGCGCY 2 cut(s) 2235, 3715
BstHHI GCGC 4 cut(s) 667, 2234, 3714, 5015
BstNI CCWGG 8 cut(s) 1463, 2381, 3222, 3285, 3297, 3622, 4083, 4223
BstPAI GACNNNNGTC 1 cut(s) 1632
BstSFI CTRYAG 5 cut(s) 116, 891, 2189, 3260, 4743
BstV2I GAAGAC 7 cut(s) 2138, 2270, 2319, 2694, 2912, 3198, 3222
BstXI CCANNNNNNTGG 1 cut(s) 5547
Bsu15I ATCGAT 2 cut(s) 2174, 3762
Bsu36I CCTNAGG 1 cut(s) 1835
BsuRI GGCC 7 cut(s) 1708, 2544, 3300, 3333, 4974, 5454, 5595
BsuTUI ATCGAT 2 cut(s) 2174, 3762
BtgI CCRYGG 1 cut(s) 470
BtrI CACGTC 2 cut(s) 581, 4430
BtsI GCAGTG 3 cut(s) 1985, 2409, 4740
BveI ACCTGC 2 cut(s) 2806, 3110
Cac8I GCNNGC 9 cut(s) 2230, 2393, 2481, 3552, 4476, 4500, 4606, 5294, 5498
CaiI CAGNNNCTG 1 cut(s) 3239
CciI TCATGA 2 cut(s) 388, 1516
CfoI GCGC 4 cut(s) 667, 2234, 3714, 5015
Cfr13I GGNCC 9 cut(s) 1707, 3299, 3624, 4085, 4136, 4205, 4364, 4704, 5420
ClaI ATCGAT 2 cut(s) 2174, 3762
CseI GACGC 3 cut(s) 152, 2098, 2917
DinI GGCGCC 1 cut(s) 2233
DraI TTTAAA 2 cut(s) 2838, 3718
DriI GACNNNNNGTC 1 cut(s) 3780
EaeI YGGCCR 2 cut(s) 2542, 3331
Eam1105I GACNNNNNGTC 1 cut(s) 3780
EciI GGCGGA 1 cut(s) 4733
Eco130I CCWWGG 2 cut(s) 4336, 4783
Eco147I AGGCCT 1 cut(s) 4974
Eco24I GRGCYC 2 cut(s) 2424, 5008
Eco31I GGTCTC 6 cut(s) 1929, 3767, 3986, 4259, 4479, 5127
Eco32I GATATC 2 cut(s) 591, 5480
Eco47I GGWCC 7 cut(s) 3624, 4085, 4136, 4205, 4364, 4704, 5420
Eco47III AGCGCT 1 cut(s) 3713
Eco57I CTGAAG 8 cut(s) 534, 1401, 2136, 3216, 3763, 4016, 4563, 5456
Eco81I CCTNAGG 1 cut(s) 1835
Eco88I CYCGRG 6 cut(s) 483, 1709, 2070, 3996, 4482, 5345
EcoNI CCTNNNNNAGG 2 cut(s) 1544, 2949
EcoRI GAATTC 1 cut(s) 419
EcoRII CCWGG 8 cut(s) 1461, 2379, 3220, 3283, 3295, 3620, 4081, 4221
EcoRV GATATC 2 cut(s) 591, 5480
EcoT14I CCWWGG 2 cut(s) 4336, 4783
EcoT22I ATGCAT 3 cut(s) 3394, 3604, 4476
EcoT38I GRGCYC 2 cut(s) 2424, 5008
EgeI GGCGCC 1 cut(s) 2233
EheI GGCGCC 1 cut(s) 2233
ErhI CCWWGG 2 cut(s) 4336, 4783
Esp3I CGTCTC 4 cut(s) 571, 1622, 2116, 3158
FalI AAGNNNNNCTT 4 cut(s) 1524, 1556, 4014, 4046
FaqI GGGAC 7 cut(s) 682, 787, 936, 2934, 3406, 5286, 5373
FauI CCCGC 2 cut(s) 2910, 5285
FbaI TGATCA 2 cut(s) 807, 5373
FblI GTMKAC 1 cut(s) 3322
FriOI GRGCYC 2 cut(s) 2424, 5008
FspBI CTAG 6 cut(s) 1529, 2958, 4632, 4733, 5048, 5087
GlaI GCGC 4 cut(s) 666, 2233, 3713, 5014
GsuI CTGGAG 3 cut(s) 1065, 2643, 3324
HaeII RGCGCY 2 cut(s) 2235, 3715
HaeIII GGCC 7 cut(s) 1708, 2544, 3300, 3333, 4974, 5454, 5595
HapII CCGG 6 cut(s) 319, 908, 2545, 3921, 4294, 4856
HgaI GACGC 3 cut(s) 152, 2098, 2917
HhaI GCGC 4 cut(s) 667, 2234, 3714, 5015
Hin1I GRCGYC 3 cut(s) 1615, 2109, 2232
Hin6I GCGC 4 cut(s) 665, 2232, 3712, 5013
HinP1I GCGC 4 cut(s) 665, 2232, 3712, 5013
HincII GTYRAC 3 cut(s) 281, 367, 1167
HindII GTYRAC 3 cut(s) 281, 367, 1167
HindIII AAGCTT 4 cut(s) 1561, 2087, 2430, 3080
HpaII CCGG 6 cut(s) 319, 908, 2545, 3921, 4294, 4856
Hpy99I CGWCG 2 cut(s) 1617, 2391
HpyCH4IV ACGT 6 cut(s) 580, 1615, 1727, 1823, 3327, 4429
HpySE526I ACGT 6 cut(s) 580, 1615, 1727, 1823, 3327, 4429
Hsp92I GRCGYC 3 cut(s) 1615, 2109, 2232
HspAI GCGC 4 cut(s) 665, 2232, 3712, 5013
KasI GGCGCC 1 cut(s) 2231
Kpn2I TCCGGA 1 cut(s) 4293
KpnI GGTACC 1 cut(s) 4569
Ksp22I TGATCA 2 cut(s) 807, 5373
LguI GCTCTTC 1 cut(s) 4032
MaeI CTAG 6 cut(s) 1529, 2958, 4632, 4733, 5048, 5087
MaeII ACGT 6 cut(s) 580, 1615, 1727, 1823, 3327, 4429
MbiI CCGCTC 1 cut(s) 3248
MfeI CAATTG 3 cut(s) 1140, 2150, 3838
MhlI GDGCHC 4 cut(s) 1539, 2424, 2581, 5008
MlsI TGGCCA 1 cut(s) 3333
MluNI TGGCCA 1 cut(s) 3333
Mly113I GGCGCC 1 cut(s) 2232
MmeI TCCRAC 5 cut(s) 1206, 1405, 4652, 5141, 5238
Mox20I TGGCCA 1 cut(s) 3333
Mph1103I ATGCAT 3 cut(s) 3394, 3604, 4476
MroI TCCGGA 1 cut(s) 4293
MroXI GAANNNNTTC 1 cut(s) 5115
MscI TGGCCA 1 cut(s) 3333
MslI CAYNNNNRTG 1 cut(s) 3285
Msp20I TGGCCA 1 cut(s) 3333
MspA1I CMGCKG 3 cut(s) 4748, 4960, 5299
MspCI CTTAAG 2 cut(s) 1213, 1828
MspI CCGG 6 cut(s) 319, 908, 2545, 3921, 4294, 4856
MunI CAATTG 3 cut(s) 1140, 2150, 3838
MvaI CCWGG 8 cut(s) 1463, 2381, 3222, 3285, 3297, 3622, 4083, 4223
NarI GGCGCC 1 cut(s) 2232
NciI CCSGG 2 cut(s) 2546, 3921
NmeAIII GCCGAG 2 cut(s) 600, 2963
NmuCI GTSAC 6 cut(s) 1098, 1947, 3142, 3799, 4129, 4778
NsiI ATGCAT 3 cut(s) 3394, 3604, 4476
PaeI GCATGC 2 cut(s) 4478, 5500
PaeR7I CTCGAG 3 cut(s) 483, 2070, 4482
PagI TCATGA 2 cut(s) 388, 1516
PceI AGGCCT 1 cut(s) 4974
PciI ACATGT 5 cut(s) 2791, 3169, 4879, 5569, 5586
PciSI GCTCTTC 1 cut(s) 4032
PcsI WCGNNNNNNNCGW 1 cut(s) 4516
PdmI GAANNNNTTC 1 cut(s) 5115
PflFI GACNNNGTC 2 cut(s) 188, 2693
PflMI CCANNNNNTGG 1 cut(s) 4815
PluTI GGCGCC 1 cut(s) 2235
PscI ACATGT 5 cut(s) 2791, 3169, 4879, 5569, 5586
PshAI GACNNNNGTC 1 cut(s) 1632
PshBI ATTAAT 1 cut(s) 1347
PsiI TTATAA 1 cut(s) 750
Psp6I CCWGG 8 cut(s) 1461, 2379, 3220, 3283, 3295, 3620, 4081, 4221
PspGI CCWGG 8 cut(s) 1461, 2379, 3220, 3283, 3295, 3620, 4081, 4221
PspPI GGNCC 9 cut(s) 1707, 3299, 3624, 4085, 4136, 4205, 4364, 4704, 5420
PspXI VCTCGAGB 1 cut(s) 483
PsrI GAACNNNNNNTAC 2 cut(s) 888, 920
PstI CTGCAG 2 cut(s) 3264, 4747
PstNI CAGNNNCTG 1 cut(s) 3239
PsyI GACNNNGTC 2 cut(s) 188, 2693
PvuII CAGCTG 3 cut(s) 4748, 4960, 5299
RseI CAYNNNNRTG 1 cut(s) 3285
SapI GCTCTTC 1 cut(s) 4032
Sau96I GGNCC 9 cut(s) 1707, 3299, 3624, 4085, 4136, 4205, 4364, 4704, 5420
SduI GDGCHC 4 cut(s) 1539, 2424, 2581, 5008
SfcI CTRYAG 5 cut(s) 116, 891, 2189, 3260, 4743
SfoI GGCGCC 1 cut(s) 2233
Sfr274I CTCGAG 3 cut(s) 483, 2070, 4482
SinI GGWCC 7 cut(s) 3624, 4085, 4136, 4205, 4364, 4704, 5420
SlaI CTCGAG 3 cut(s) 483, 2070, 4482
SmiMI CAYNNNNRTG 1 cut(s) 3285
SmlI CTYRAG 9 cut(s) 137, 483, 1213, 1828, 2070, 2448, 4482, 4526, 5131
SmoI CTYRAG 9 cut(s) 137, 483, 1213, 1828, 2070, 2448, 4482, 4526, 5131
SpeI ACTAGT 1 cut(s) 4631
SphI GCATGC 2 cut(s) 4478, 5500
SseBI AGGCCT 1 cut(s) 4974
SspDI GGCGCC 1 cut(s) 2231
SspI AATATT 2 cut(s) 254, 2734
SspMI CTAG 6 cut(s) 1529, 2958, 4632, 4733, 5048, 5087
StuI AGGCCT 1 cut(s) 4974
StyI CCWWGG 2 cut(s) 4336, 4783
TaiI ACGT 6 cut(s) 583, 1618, 1730, 1826, 3330, 4432
TaqII GACCGA 1 cut(s) 5109
TatI WGTACW 4 cut(s) 1314, 3371, 3903, 5127
TauI GCSGC 3 cut(s) 161, 2264, 3946
TseFI GTSAC 6 cut(s) 1098, 1947, 3142, 3799, 4129, 4778
Tsp45I GTSAC 6 cut(s) 1098, 1947, 3142, 3799, 4129, 4778
TspGWI ACGGA 6 cut(s) 251, 459, 671, 2432, 3259, 4525
Tth111I GACNNNGTC 2 cut(s) 188, 2693
Van91I CCANNNNNTGG 1 cut(s) 4815
Vha464I CTTAAG 2 cut(s) 1213, 1828
VpaK11BI GGWCC 7 cut(s) 3624, 4085, 4136, 4205, 4364, 4704, 5420
VspI ATTAAT 1 cut(s) 1347
XagI CCTNNNNNAGG 2 cut(s) 1544, 2949
XbaI TCTAGA 2 cut(s) 5047, 5086
XcmI CCANNNNNNNNNTGG 2 cut(s) 1023, 2604
XhoI CTCGAG 3 cut(s) 483, 2070, 4482
XmiI GTMKAC 1 cut(s) 3322
XmnI GAANNNNTTC 1 cut(s) 5115
XspI CTAG 6 cut(s) 1529, 2958, 4632, 4733, 5048, 5087
ZraI GACGTC 1 cut(s) 1616
Zsp2I ATGCAT 3 cut(s) 3394, 3604, 4476
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.