Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0003960

Physical Location & Seq

Genomic Coordinates Forward (+)

32487816 .. 32489480

Gene Structure 1665 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1258700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1665 bp

ATGGCAGAACAAATTGTATGCTTTGTGATCGACAAGTTAATTCAACTACTCATCACCACAGAAGCAAACCTTTCAAGAGACGTACGCAAAGAAGTTGGTTTCATCAAAGACGAGCTAGATAGCATCCGATCATTCCTCAAGGACGCAGATGTTAAGGCTGCAGCAGATGGTGAGAGGACAAATGACAGCGTCAAAACTTGGGTCAACCAAGTAAGAGAAGCAGCTTTTTACATTGAAGATGTCATTGATGAATATTTGCTCCGCATCACACGGCATCATCAAGATCGTGGATTCGTTCGTTTTTACAACAAAATTATTTGGTTGGCTAAGAGAATGAGATCACGTGATGGGATAGTTTTGAAAGTTGACAGTATGAAGGAATTGGTTCGTGAAATCAAGGCAAGAAGTGAAAGATACGAATTCAGTTCCATTGACCAGCAAGGTCAAGATAATGGCGAAAAGACTCTTCCGTGGCATGACCCTCGAGTGGCTTCGCTTTTCATTGAGGAAGCTGAAGTTGTGGGGATTGAATCTGCCAGAGCAAAGCTGACTAATTTGCTGGTCGGAGGAGCACCGAGACGCGAAGTGATATCAGTGGTTGGCATGGGAGGACTTGGGAAGACCACTCTAGCCAAGAAAGTTTATGACAGTCAGACGGTGATGGGCCACTTTGATTGCTACGCTTGGATTCCGGTGTCCCAGACATATAGGGTGGAGGATCTTCTGAGGAAGGTAATCAAAATGTTCTACAATTCAAGTAAGGAGAATTTCCCAGAGGGAATTGACACAATGGATGAGGAGTCCCTAATCAGAAAATCAAGAGAGTATTTGCAGCAAAAGAGGTATGTCGTTGTGTTTGATGATGTGTGGAAAGTAGATTTTTGGGGGGCTATAGAACATGCTTTACCGGATAATAAAGGCGGACGAATAATGATTACTACAAGGATTCAAGATGTTGCCGATTTTTGCAAAAAATCTTGTTTTGTTCATGTTCATCACTTGCAACCTCTGTCTCCAACAAATGCATGGAAATTGTTTTGTAGAAAGGCATTCCAGTTTGAATTGGAGGGAAATTGCCCTCCAGAGTTAGAAGATATGTCACGGGAAATCATTAAAAAGTGCGAGGGACTACCTTTGGCAATTGTTTGCATAGGTGGTCTGCTGTCAACGAAAGTTAAAGGTGTGAATGAATGGCAGAAGTTATACAATAGCTTGAGTTCAGAGTTACAAAGCAATCCCCATCTTACAAGCCTCACAAGAATCCTATCCCTCAGCTATCACCATCTACCATATTACCTCAAATCATGTGTCTTGTACTTCGGCATATTTCCAGAGGACTACTCAATTAATTGCGTAAGATTGATTCGGTTGTGGATTGCTGAAGGGTTTGTAAAATTGAAGAAGGGAAAGACACTAGAAGAGGTTGGAGAGGAATACTTGACTGAGCTAATTCACAGAAGCCTGGTTCAAGTGTCAAAGGTTTACAGGGATGGAAAAGCTAGAGAATGTCGAGTTCATGATCTTTTCTATGAGGTCCTCCTTAAAATGTGTGCGGATTCATGCTTTTGTCATGTGTTATCAAAAGACGAGTCAGCTTTTAAGCCAACAACTCGACGTCTCTCAATGGCCATTAGTCCCTCTGATGCCTTGGGAAGCATTACATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0002376 GO:0003674 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0008219 GO:0009626 GO:0009987 GO:0012501 GO:0016020 GO:0019897 GO:0019898 GO:0023052 GO:0033554 GO:0034050 GO:0036094 GO:0044425 GO:0044459 GO:0044464 GO:0045087 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071944 GO:0097159 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko04626 map04626

Pfam Domains

Protein Families

NB-ARC

Protein Analysis

555

Amino Acids

63.7

Weight (kDa)

7.84

Isoelectric Point (pI)

41.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Rx_N	PF18052	5 - 100	1.8e-22	Rx N-terminal domain
NB-ARC	PF00931	182 - 354	5.1e-43	NB-ARC domain
WHD_DRP	PF23559	442 - 511	1.1e-24	Disease resistance protein Winged helix domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003960)

Species	Orthologous Gene IDs
malus_domestica	MD00G1097800.v1.1 MD01G1186500.v1.1 MD01G1186600.v1.1 MD01G1186800.v1.1 MD01G1187000.v1.1 MD01G1187100.v1.1 MD07G1258600.v1.1 MD07G1258700.v1.1 MD07G1258800.v1.1 MD07G1258900.v1.1 MD07G1259200.v1.1 MD07G1259300.v1.1
prunus_persica	Prupe.2G283200_v2.0.a1 Prupe.2G283300_v2.0.a1 Prupe.2G283400_v2.0.a1
pyrus_communis	pycom01g19810 pycom01g19820 pycom01g19840 pycom01g19900 pycom07g23170 pycom07g23230 pycom07g23240 pycom07g23250 pycom07g23260
rosa_chinensis	RchiOBHm_Chr1g0375241
rosa_laevigata	RLG00000026676
rosa_multiflora	Rmu_co8330553.1_g000001 Rmu_sc0000554.1_g000032
rosa_roxburghii	Rroxscaffold_4G00282910
rosa_rugosa	Rorug01G0389400
rosa_samantha	Rh1AG400100 Rh1BG364400 Rh1DG395000
rosa_wichuraiana	Rw1G035520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1618
AccII	CGCG	1 cut(s)	582
AciI	CCGC	3 cut(s)	262, 921, 1553
AclWI	GGATC	1 cut(s)	726
AcoI	YGGCCR	1 cut(s)	1626
AcsI	RAATTY	2 cut(s)	419, 766
AcuI	CTGAAG	2 cut(s)	534, 1401
AcvI	CACGTG	1 cut(s)	344
AcyI	GRCGYC	1 cut(s)	1615
AfaI	GTAC	2 cut(s)	84, 1316
AfiI	CCNNNNNNNGG	1 cut(s)	487
AjnI	CCWGG	1 cut(s)	1461
AluBI	AGCT	9 cut(s)	115, 224, 512, 547, 1212, 1275, 1447, 1499, 1595
AluI	AGCT	9 cut(s)	115, 224, 512, 547, 1212, 1275, 1447, 1499, 1595
Alw21I	GWGCWC	1 cut(s)	574
Alw26I	GTCTC	4 cut(s)	72, 571, 1017, 1622
AlwI	GGATC	1 cut(s)	726
Ama87I	CYCGRG	1 cut(s)	483
AoxI	GGCC	2 cut(s)	664, 1626
ApeKI	GCWGC	4 cut(s)	158, 161, 221, 832
ApoI	RAATTY	2 cut(s)	419, 766
ArsI	GACNNNNNNTTYG	6 cut(s)	186, 218, 1293, 1325, 1574, 1606
AseI	ATTAAT	1 cut(s)	1347
Asp700I	GAANNNNTTC	1 cut(s)	384
AspS9I	GGNCC	2 cut(s)	664, 1534
AsuHPI	GGTGA	4 cut(s)	46, 182, 670, 1271
AvaI	CYCGRG	1 cut(s)	483
AvaII	GGWCC	1 cut(s)	1534
BalI	TGGCCA	1 cut(s)	1628
BbrPI	CACGTG	1 cut(s)	344
BbsI	GAAGAC	1 cut(s)	626
Bbv12I	GWGCWC	1 cut(s)	574
BbvCI	CCTCAGC	1 cut(s)	1271
BbvI	GCAGC	4 cut(s)	145, 173, 233, 844
BccI	CCATC	6 cut(s)	161, 341, 655, 1248, 1290, 1484
BceAI	ACGGC	1 cut(s)	287
BcgI	CGANNNNNNTGC	2 cut(s)	464, 498
BciT130I	CCWGG	1 cut(s)	1463
BcoDI	GTCTC	4 cut(s)	72, 571, 1017, 1622
BfaI	CTAG	4 cut(s)	116, 629, 1415, 1500
BfmI	CTRYAG	2 cut(s)	159, 891
BisI	GCNGC	4 cut(s)	159, 162, 222, 833
BlsI	GCNGC	4 cut(s)	160, 163, 223, 834
Bme1390I	CCNGG	1 cut(s)	1463
Bme18I	GGWCC	1 cut(s)	1534
BmeT110I	CYCGRG	1 cut(s)	483
BmgT120I	GGNCC	2 cut(s)	664, 1534
BmrFI	CCNGG	1 cut(s)	1463
BmsI	GCATC	4 cut(s)	132, 273, 283, 1633
BpiI	GAAGAC	1 cut(s)	626
BplI	GAGNNNNNCTC	2 cut(s)	1325, 1357
BpmI	CTGGAG	1 cut(s)	1065
Bpu10I	CCTNAGC	1 cut(s)	1271
BpuEI	CTTGAG	2 cut(s)	122, 1234
BsaAI	YACGTR	1 cut(s)	344
BsaHI	GRCGYC	1 cut(s)	1615
BsaJI	CCNNGG	2 cut(s)	470, 1647
BsaWI	WCCGGW	2 cut(s)	691, 907
Bsc4I	CCNNNNNNNGG	1 cut(s)	487
Bse1I	ACTGG	1 cut(s)	1054
BseBI	CCWGG	1 cut(s)	1463
BseDI	CCNNGG	2 cut(s)	470, 1647
BseGI	GGATG	3 cut(s)	123, 799, 1495
BseLI	CCNNNNNNNGG	1 cut(s)	487
BseMII	CTCAG	3 cut(s)	716, 1285, 1434
BseNI	ACTGG	1 cut(s)	1054
BseRI	GAGGAG	2 cut(s)	582, 812
BseXI	GCAGC	4 cut(s)	145, 173, 233, 844
Bsh1236I	CGCG	1 cut(s)	582
BshFI	GGCC	2 cut(s)	666, 1628
BsiHKAI	GWGCWC	1 cut(s)	574
BsiHKCI	CYCGRG	1 cut(s)	483
BsiSI	CCGG	2 cut(s)	692, 908
BsiWI	CGTACG	1 cut(s)	82
BslFI	GGGAC	4 cut(s)	682, 787, 1140, 1620
BslI	CCNNNNNNNGG	1 cut(s)	487
BsmAI	GTCTC	4 cut(s)	72, 571, 1017, 1622
BsmBI	CGTCTC	3 cut(s)	72, 571, 1622
BsmFI	GGGAC	4 cut(s)	682, 787, 1140, 1620
BsmI	GAATGC	1 cut(s)	1049
BsnI	GGCC	2 cut(s)	666, 1628
BsoBI	CYCGRG	1 cut(s)	483
Bsp1286I	GDGCHC	1 cut(s)	574
Bsp143I	GATC	6 cut(s)	27, 128, 283, 338, 718, 1519
BspACI	CCGC	3 cut(s)	262, 921, 1553
BspANI	GGCC	2 cut(s)	666, 1628
BspCNI	CTCAG	3 cut(s)	717, 1284, 1435
BspFNI	CGCG	1 cut(s)	582
BspHI	TCATGA	1 cut(s)	1516
BspMAI	CTGCAG	1 cut(s)	163
BspPI	GGATC	1 cut(s)	726
BsrI	ACTGG	1 cut(s)	1054
BssECI	CCNNGG	2 cut(s)	470, 1647
BssMI	GATC	6 cut(s)	27, 128, 283, 338, 718, 1519
BssNI	GRCGYC	1 cut(s)	1615
BssT1I	CCWWGG	1 cut(s)	1647
Bst2UI	CCWGG	1 cut(s)	1463
Bst4CI	ACNGT	3 cut(s)	371, 650, 658
Bst6I	CTCTTC	2 cut(s)	471, 1413
BstACI	GRCGYC	1 cut(s)	1615
BstBAI	YACGTR	1 cut(s)	344
BstDEI	CTNAG	4 cut(s)	327, 725, 1271, 1443
BstDSI	CCRYGG	1 cut(s)	470
BstF5I	GGATG	3 cut(s)	123, 799, 1495
BstFNI	CGCG	1 cut(s)	582
BstKTI	GATC	6 cut(s)	30, 131, 286, 341, 721, 1522
BstMAI	GTCTC	4 cut(s)	72, 571, 1017, 1622
BstMBI	GATC	6 cut(s)	27, 128, 283, 338, 718, 1519
BstNI	CCWGG	1 cut(s)	1463
BstNSI	RCATGY	1 cut(s)	902
BstSCI	CCNGG	1 cut(s)	1461
BstSFI	CTRYAG	2 cut(s)	159, 891
BstUI	CGCG	1 cut(s)	582
BstV1I	GCAGC	4 cut(s)	145, 173, 233, 844
BstV2I	GAAGAC	1 cut(s)	626
BstX2I	RGATCY	1 cut(s)	718
BstYI	RGATCY	1 cut(s)	718
BsuRI	GGCC	2 cut(s)	666, 1628
BtgI	CCRYGG	1 cut(s)	470
BtsCI	GGATG	3 cut(s)	123, 799, 1495
BtsIMutI	CAGTG	1 cut(s)	600
CciI	TCATGA	1 cut(s)	1516
Cfr13I	GGNCC	2 cut(s)	664, 1534
CseI	GACGC	3 cut(s)	152, 178, 588
Csp6I	GTAC	2 cut(s)	83, 1315
CspCI	CAANNNNNGTGG	2 cut(s)	656, 691
CviAII	CATG	9 cut(s)	476, 604, 899, 989, 1026, 1305, 1517, 1560, 1571
CviQI	GTAC	2 cut(s)	83, 1315
DdeI	CTNAG	4 cut(s)	327, 725, 1271, 1443
DpnI	GATC	6 cut(s)	29, 130, 285, 340, 720, 1521
DpnII	GATC	6 cut(s)	27, 128, 283, 338, 718, 1519
EaeI	YGGCCR	1 cut(s)	1626
Eam1104I	CTCTTC	2 cut(s)	471, 1413
EarI	CTCTTC	2 cut(s)	471, 1413
EciI	GGCGGA	1 cut(s)	936
Eco130I	CCWWGG	1 cut(s)	1647
Eco32I	GATATC	1 cut(s)	591
Eco47I	GGWCC	1 cut(s)	1534
Eco57I	CTGAAG	2 cut(s)	534, 1401
Eco72I	CACGTG	1 cut(s)	344
Eco88I	CYCGRG	1 cut(s)	483
EcoO109I	RGGNCCY	1 cut(s)	1534
EcoRI	GAATTC	1 cut(s)	419
EcoRII	CCWGG	1 cut(s)	1461
EcoRV	GATATC	1 cut(s)	591
EcoT14I	CCWWGG	1 cut(s)	1647
EcoT22I	ATGCAT	1 cut(s)	1027
ErhI	CCWWGG	1 cut(s)	1647
Esp3I	CGTCTC	3 cut(s)	72, 571, 1622
FaeI	CATG	9 cut(s)	479, 607, 902, 992, 1029, 1308, 1520, 1563, 1574
FalI	AAGNNNNNCTT	2 cut(s)	54, 86
FaqI	GGGAC	4 cut(s)	682, 787, 1140, 1620
FatI	CATG	9 cut(s)	475, 603, 898, 988, 1025, 1304, 1516, 1559, 1570
Fnu4HI	GCNGC	4 cut(s)	159, 162, 222, 833
FokI	GGATG	3 cut(s)	110, 806, 1502
Fsp4HI	GCNGC	4 cut(s)	159, 162, 222, 833
FspBI	CTAG	4 cut(s)	116, 629, 1415, 1500
GluI	GCNGC	4 cut(s)	159, 162, 222, 833
GsuI	CTGGAG	1 cut(s)	1065
HaeIII	GGCC	2 cut(s)	666, 1628
HapII	CCGG	2 cut(s)	692, 908
HgaI	GACGC	3 cut(s)	152, 178, 588
Hin1I	GRCGYC	1 cut(s)	1615
Hin1II	CATG	9 cut(s)	479, 607, 902, 992, 1029, 1308, 1520, 1563, 1574
HincII	GTYRAC	3 cut(s)	205, 367, 1167
HindII	GTYRAC	3 cut(s)	205, 367, 1167
HpaII	CCGG	2 cut(s)	692, 908
HphI	GGTGA	4 cut(s)	46, 182, 670, 1271
Hpy166II	GTNNAC	4 cut(s)	205, 367, 1167, 1483
Hpy188I	TCNGA	7 cut(s)	128, 566, 654, 726, 812, 1222, 1642
Hpy188III	TCNNGA	9 cut(s)	75, 281, 389, 446, 819, 950, 1082, 1331, 1517
Hpy8I	GTNNAC	4 cut(s)	205, 367, 1167, 1483
Hpy99I	CGWCG	1 cut(s)	1617
HpyAV	CCTTC	4 cut(s)	370, 724, 1376, 1396
HpyCH4III	ACNGT	3 cut(s)	371, 650, 658
HpyCH4IV	ACGT	3 cut(s)	81, 343, 1615
HpyCH4V	TGCA	6 cut(s)	161, 832, 969, 1003, 1025, 1149
HpyF3I	CTNAG	4 cut(s)	327, 725, 1271, 1443
HpySE526I	ACGT	3 cut(s)	81, 343, 1615
Hsp92I	GRCGYC	1 cut(s)	1615
Hsp92II	CATG	9 cut(s)	479, 607, 902, 992, 1029, 1308, 1520, 1563, 1574
Kzo9I	GATC	6 cut(s)	27, 128, 283, 338, 718, 1519
LmnI	GCTCC	2 cut(s)	264, 569
Lsp1109I	GCAGC	4 cut(s)	145, 173, 233, 844
LweI	GCATC	4 cut(s)	132, 273, 283, 1633
MaeI	CTAG	4 cut(s)	116, 629, 1415, 1500
MaeII	ACGT	3 cut(s)	81, 343, 1615
MaeIII	GTNAC	2 cut(s)	1098, 1224
MalI	GATC	6 cut(s)	29, 130, 285, 340, 720, 1521
MboI	GATC	6 cut(s)	27, 128, 283, 338, 718, 1519
MboII	GAAGA	7 cut(s)	248, 458, 631, 713, 1103, 1411, 1430
MfeI	CAATTG	1 cut(s)	1140
MflI	RGATCY	1 cut(s)	718
MhlI	GDGCHC	1 cut(s)	574
MlsI	TGGCCA	1 cut(s)	1628
MluNI	TGGCCA	1 cut(s)	1628
MlyI	GAGTC	3 cut(s)	457, 809, 1598
MmeI	TCCRAC	3 cut(s)	544, 1040, 1405
Mox20I	TGGCCA	1 cut(s)	1628
Mph1103I	ATGCAT	1 cut(s)	1027
MroXI	GAANNNNTTC	1 cut(s)	384
MscI	TGGCCA	1 cut(s)	1628
MseI	TTAA	7 cut(s)	38, 153, 1113, 1176, 1347, 1542, 1599
Msp20I	TGGCCA	1 cut(s)	1628
MspI	CCGG	2 cut(s)	692, 908
MspR9I	CCNGG	1 cut(s)	1463
MunI	CAATTG	1 cut(s)	1140
Mva1269I	GAATGC	1 cut(s)	1049
MvaI	CCWGG	1 cut(s)	1463
MvnI	CGCG	1 cut(s)	582
NdeII	GATC	6 cut(s)	27, 128, 283, 338, 718, 1519
NlaIII	CATG	9 cut(s)	479, 607, 902, 992, 1029, 1308, 1520, 1563, 1574
NmuCI	GTSAC	1 cut(s)	1098
NsiI	ATGCAT	1 cut(s)	1027
NspI	RCATGY	1 cut(s)	902
PaeR7I	CTCGAG	1 cut(s)	483
PagI	TCATGA	1 cut(s)	1516
PctI	GAATGC	1 cut(s)	1049
PdmI	GAANNNNTTC	1 cut(s)	384
PfeI	GAWTC	7 cut(s)	291, 530, 688, 946, 1260, 1363, 1556
Pfl23II	CGTACG	1 cut(s)	82
PflFI	GACNNNGTC	1 cut(s)	188
PkrI	GCNGC	4 cut(s)	160, 163, 223, 834
PleI	GAGTC	3 cut(s)	457, 808, 1597
PmaCI	CACGTG	1 cut(s)	344
PmlI	CACGTG	1 cut(s)	344
PpsI	GAGTC	3 cut(s)	457, 808, 1597
Ppu21I	YACGTR	1 cut(s)	344
PpuMI	RGGWCCY	1 cut(s)	1534
PshBI	ATTAAT	1 cut(s)	1347
Psp5II	RGGWCCY	1 cut(s)	1534
Psp6I	CCWGG	1 cut(s)	1461
PspCI	CACGTG	1 cut(s)	344
PspGI	CCWGG	1 cut(s)	1461
PspLI	CGTACG	1 cut(s)	82
PspPI	GGNCC	2 cut(s)	664, 1534
PspPPI	RGGWCCY	1 cut(s)	1534
PspXI	VCTCGAGB	1 cut(s)	483
PsrI	GAACNNNNNNTAC	2 cut(s)	888, 920
PstI	CTGCAG	1 cut(s)	163
PsuI	RGATCY	1 cut(s)	718
PsyI	GACNNNGTC	1 cut(s)	188
RsaI	GTAC	2 cut(s)	84, 1316
RsaNI	GTAC	2 cut(s)	83, 1315
SaqAI	TTAA	7 cut(s)	38, 153, 1113, 1176, 1347, 1542, 1599
SatI	GCNGC	4 cut(s)	159, 162, 222, 833
Sau3AI	GATC	6 cut(s)	27, 128, 283, 338, 718, 1519
Sau96I	GGNCC	2 cut(s)	664, 1534
SchI	GAGTC	3 cut(s)	457, 809, 1598
ScrFI	CCNGG	1 cut(s)	1463
SduI	GDGCHC	1 cut(s)	574
SfaNI	GCATC	4 cut(s)	132, 273, 283, 1633
SfcI	CTRYAG	2 cut(s)	159, 891
Sfr274I	CTCGAG	1 cut(s)	483
SinI	GGWCC	1 cut(s)	1534
SlaI	CTCGAG	1 cut(s)	483
SmlI	CTYRAG	3 cut(s)	137, 483, 1213
SmoI	CTYRAG	3 cut(s)	137, 483, 1213
SsiI	CCGC	3 cut(s)	262, 921, 1553
SspI	AATATT	1 cut(s)	254
SspMI	CTAG	4 cut(s)	116, 629, 1415, 1500
StyD4I	CCNGG	1 cut(s)	1461
StyI	CCWWGG	1 cut(s)	1647
TaaI	ACNGT	3 cut(s)	371, 650, 658
TaiI	ACGT	3 cut(s)	84, 346, 1618
TaqI	TCGA	4 cut(s)	30, 484, 1510, 1612
TatI	WGTACW	1 cut(s)	1314
TfiI	GAWTC	7 cut(s)	291, 530, 688, 946, 1260, 1363, 1556
Tru1I	TTAA	7 cut(s)	38, 153, 1113, 1176, 1347, 1542, 1599
Tru9I	TTAA	7 cut(s)	38, 153, 1113, 1176, 1347, 1542, 1599
TscAI	CASTG	1 cut(s)	600
TseFI	GTSAC	1 cut(s)	1098
TseI	GCWGC	4 cut(s)	158, 161, 221, 832
Tsp45I	GTSAC	1 cut(s)	1098
TspDTI	ATGAA	9 cut(s)	91, 264, 389, 490, 977, 983, 1203, 1505, 1548
TspGWI	ACGGA	1 cut(s)	459
TspRI	CASTG	1 cut(s)	600
Tth111I	GACNNNGTC	1 cut(s)	188
VpaK11BI	GGWCC	1 cut(s)	1534
VspI	ATTAAT	1 cut(s)	1347
XapI	RAATTY	2 cut(s)	419, 766
XceI	RCATGY	1 cut(s)	902
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1023
XhoI	CTCGAG	1 cut(s)	483
XmnI	GAANNNNTTC	1 cut(s)	384
XspI	CTAG	4 cut(s)	116, 629, 1415, 1500
ZraI	GACGTC	1 cut(s)	1616
Zsp2I	ATGCAT	1 cut(s)	1027

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.