Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0003960

Physical Location & Seq

Genomic Coordinates Forward (+)

28550421 .. 28562418

Gene Structure 11998 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1186800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3360 bp

ATGGCAGAATCAGTTGTATGCTTTGTGATCGATAAGTTAATTTCACTACTCGTCACCACAGAAGCGAAACTTTCAAGAGACGTGCGTGCTGAAGTTGGTTTTCTCAGAGATGAACTTGAAAGCATCCGATCATTCCTCAAGGATGCAGACGCTAAAGCAGCAGCAGCAGCAGCAGAAGGAGACATGGTGACTGACAGTGCCAAAACTTGGGTCAAACAAGTAAGGGAAGCAGCTTTTTACATTGAAGATGTCATCGATGAGTATTTGCTTCGTGTCACAAGGCACCATCAAGACCGCGGATTCGTTCGCTTTGTCCACAAAGTTGTTTGGTTTCTGAAGAAAATGAAACCACAGGATGAGATAGCCTCAAAAATAGAGGGCATGAGAGCATTGGTTAGTGAAATCAAGGCTAGACATGAAAGATATGGGCTTAGTTCAATTGATCAGCAAGGTCAAAGCAGTGAAGAAATTGTTGTTTCGTGGCATGACCCTCGAGTGGCTTCCCTTTTCATCGAGGAAGCTGAAGTTGTTGGTGTGGAATCTGCCAGAGATGAGCTGATTAGTTGGTTGGTAGGAAAAGCATCAAGAGGCGCATACCGTGAAGTAATATCAGTGGTTGGCATGGGAGGACTTGGTAAGACCACTCTCGCCAAGAAAGTCTATGACAACCAAAGGGTGATCGAACACTTCGATTGCCATGCTTGGATTACAGTGTCTCAGACGTACAAGGTGGAGGATCTTCTGAGGCAGATGGTAATGCAATTCTACAAGGCCAGGAAGGAATATACCCCGGAGGGCATTGACACAATGGAAAAAGAATCTTTAATTAGAAAATCAAGAGAATTACTGCAGCAAAAGAGAAGCAAAACTTCCAAGAGATCAAACCTTTCGAGAAATGCACGTATTGAAGTTGGTTTCATCAAAGATGAACTCGAGAGCATCCGATCATTCCTCAAGGAAGCAGATGCTAAGGCTGCAGCAGCAGATGGAGAGACTATGAACGACGGTGTTAAAACTTGGGTCAATCAAGTAAGAGAAGCAGCCTTTTACATTGAAGATGTCATCGATGAATATTTGCTCCACATCATACAGCATCATCAACACCATGGATTCATTCGTTTTTTCCATAACATAATCCGGTTGGTTCAAAAAATGAGATCACAGGATGGGATAGTTTCGAAAGTTGACGGTATAAAAAAATTGGTTCGTGAAATCAAGGATAGAAGTGAAAGATATGGATTCAAATCTATTGATCAGCAAGGGCAGAGCAGTGTAGAAAAGACTCTACCGTCGAACGACCCTCGAGTGGCTTCCCTTTTCATTGAGGAAGCTGAAATTGTGGGGATTGAATCTGCCAGAGATGAGCTAACTCGTTTGTTGGTAGAAGGAGCGCCAAGACGTGAAGTGATATCAGTGGTTGGCATGGGAGGACTTGGGAAAACCACTCTTGCCAAGAAAGTGTATGACCAGCCGAAGGTGATGGCACACTTTGATTGCTATGCTTGGATTACAGTGTCCCAGTCGTACAGGGTTGAGGATCTTCTGAGGACGGTAATAAAAAAGTTCTACAGTTCAAGGAAGGAGCGTTTCCCAGAGGAAATTGACACAATGGATGAGGAGTCCCTGATTAGCACATCTAGAGAGTATTTGCAGCAAAAGAGGTACATTGTTGTGTTTGATGATGTGTGGAAAGTAGATTTTTGGGGGGCAATAGAACATGCTTTACCCGATAGTAAAGGCGGACGAATAATTATCACTACACGGATTCAAGATGTTGCCAACTTTTGCAAAAGATCTTGTTTTGTTCATGTTCATCACTTGCAGCATCTGCCTCCAAACAAGGCATGGGAATTGTTTTGTAGGAAGGCATTTCAGTTTGAATTGGAGGGAAAGTGTCCTGAAGAGTTGGAAGAGTTGTCTCTTAACATCGTCAAAAGATGCGAAGGACTACCTTTGGCAATTGTTTCCGTAGGTGGTCTGCTGTCAACCAAAGCTAAAGTTGTGTCTGAATGGCAGAAGTTATACAATAGCTTAAGTTCAGAGTTAGAAAGAAATCCCCACCTTACAAGCCTCACAAGAATCCTATCCCTCAGCTATCACCATCTACCGTATTACCTCAAATCGTGTGTATTATACTTTGGCATCTTTCCCGAGGATTACTCAGTTAATTGCATAAGATTAGTTCGGTTGTGGATCGCTGAAGGCTTTGTGAAACCGAGGAGGGGCAAGACACTAGAAGAGGTTGGAGAGGAATACTTGATGGAGCTAATTCACAGAAGCCTGGTTCAAGTTTCAGAAGTTTACATTGATGGAAAAGCTCGAGCTTGTAGAGTTCATGATCTATTGCACGACGTCCTCCTTAAAAAGGGGCTGGATTCAAGCTTCTGTCATGTGTTATCAAAAGACGAGTCGACTTTTAAACCAACAACTCGACGTCTCTCATTGAACATCAGTCCCTCTTGGGCCCTGGGAAGCATTACACAATCTCATATTCGTTCCGTATTTACTTTCAACCTAGAAGAATGGCCCGAGTCTTTTCTCGATACATTGAGTGGAAACATTAAACTTGTGAAAGTATTGGATTTCACGGATGCTCCAATCAATCAACTTCCTAAATATGTGGGTGATTTGTATCTCTTGACGTACTTAAGCCTGAGGAATACAAAGGTGAAGTTGCTTCCAGACTCCATAGGCAATCTACAAAACTTGGAAACCCTGGATCTAAAACAGTCCTTGGTGTATGAAATACCAACCAAGATCAATAAGCTTGTTAAGCTGCGGCATCTTTTGGCCTACCATCGTGACTACAACTGCGACGTGCGTTTGTTCTTGAATATGGAAAAAGAAGTGAAGATACATGAAGGTATTGGATGCTTACAAGCTTTGCAAAAACTGTCCTATGTGGAGGCGAATCATGGAGGGATCAGGCTAATCAAAGAACTGGGAAAGTTAAGACAGCTACGGAAGTTAGGCCTAAAAAACTTGAAAAGTGAAGATGGAAGGGCTGTGTGTGCTTCTATTGAAAAGATGAACCACCTTGAATCTCTACTTTTAACTGCAATAAATGAAGATGAAGTCCTTGATCTAGAATCTATTTCAACTCCACCAGAATTTCTTCGTTCTCTCTGCTTGATAGGGCGTTTAGAGCAGTTGCCAGGTTGGATTTCAAATCTTCAACACATAGTCAAGATAACCATTTATTGGTCAAGGTTGAGAGATTCCCCACTAAAAGCCCTTCAAAATCTACCTAATCTGTTGAAGCTAACTTTGGGTAGGAATGCATATGATGGTGTGCAGCTGCATTTTGAGGAAGGAGGATTTCAGAAACTTAGGGAGCTAAGGCAAAGATGGTGGGACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0002376 GO:0003674 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0008219 GO:0009626 GO:0009987 GO:0012501 GO:0016020 GO:0019897 GO:0019898 GO:0023052 GO:0033554 GO:0034050 GO:0036094 GO:0044425 GO:0044459 GO:0044464 GO:0045087 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071944 GO:0097159 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko04626 map04626

Pfam Domains

Protein Families

NB-ARC

Protein Analysis

1120

Amino Acids

128.57

Weight (kDa)

8.23

Isoelectric Point (pI)

47.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Rx_N	PF18052	5 - 106	2.6e-21	Rx N-terminal domain
NB-ARC	PF00931	183 - 287	4.7e-19	NB-ARC domain
Rx_N	PF18052	274 - 376	4.8e-18	Rx N-terminal domain
NB-ARC	PF00931	453 - 627	7.5e-42	NB-ARC domain
WHD_DRP	PF23559	715 - 785	2e-25	Disease resistance protein Winged helix domain
LRR_14	PF23598	830 - 1114	4.7e-38	Leucine-rich repeat region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0003960)

Species	Orthologous Gene IDs
malus_domestica	MD00G1097800.v1.1 MD01G1186500.v1.1 MD01G1186600.v1.1 MD01G1186800.v1.1 MD01G1187000.v1.1 MD01G1187100.v1.1 MD07G1258600.v1.1 MD07G1258700.v1.1 MD07G1258800.v1.1 MD07G1258900.v1.1 MD07G1259200.v1.1 MD07G1259300.v1.1
prunus_persica	Prupe.2G283200_v2.0.a1 Prupe.2G283300_v2.0.a1 Prupe.2G283400_v2.0.a1
pyrus_communis	pycom01g19810 pycom01g19820 pycom01g19840 pycom01g19900 pycom07g23170 pycom07g23230 pycom07g23240 pycom07g23250 pycom07g23260
rosa_chinensis	RchiOBHm_Chr1g0375241
rosa_laevigata	RLG00000026676
rosa_multiflora	Rmu_co8330553.1_g000001 Rmu_sc0000554.1_g000032
rosa_roxburghii	Rroxscaffold_4G00282910
rosa_rugosa	Rorug01G0389400
rosa_samantha	Rh1AG400100 Rh1BG364400 Rh1DG395000
rosa_wichuraiana	Rw1G035520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1288
AatII	GACGTC	2 cut(s)	2355, 2437
AccB1I	GGYRCC	1 cut(s)	282
AccB7I	CCANNNNNTGG	2 cut(s)	207, 3201
AccI	GTMKAC	1 cut(s)	2411
AccII	CGCG	1 cut(s)	297
AciI	CCGC	4 cut(s)	295, 297, 1740, 2779
AclWI	GGATC	5 cut(s)	744, 1545, 2201, 2727, 2930
AcsI	RAATTY	1 cut(s)	3110
AcuI	CTGAAG	5 cut(s)	111, 356, 543, 1920, 2220
AcyI	GRCGYC	2 cut(s)	2352, 2434
AfaI	GTAC	4 cut(s)	725, 1526, 1664, 2645
AfiI	CCNNNNNNNGG	7 cut(s)	207, 496, 1306, 1474, 2222, 2365, 3201
AflII	CTTAAG	2 cut(s)	2032, 2647
AjiI	CACGTC	3 cut(s)	82, 1400, 2818
AjnI	CCWGG	5 cut(s)	773, 2280, 2466, 2715, 3154
AjuI	GAANNNNNNNTTGG	2 cut(s)	3251, 3283
Alw26I	GTCTC	6 cut(s)	72, 174, 720, 986, 1923, 2441
AlwI	GGATC	5 cut(s)	744, 1545, 2201, 2727, 2930
AlwNI	CAGNNNCTG	1 cut(s)	1828
Ama87I	CYCGRG	6 cut(s)	492, 932, 1302, 2150, 2319, 2528
AoxI	GGCC	5 cut(s)	771, 2463, 2525, 2790, 2971
ApaI	GGGCCC	1 cut(s)	2467
ApoI	RAATTY	1 cut(s)	3110
ArsI	GACNNNNNNTTYG	6 cut(s)	195, 227, 284, 316, 2393, 2425
Asp700I	GAANNNNTTC	1 cut(s)	3114
AspLEI	GCGC	2 cut(s)	593, 1393
AspS9I	GGNCC	3 cut(s)	2463, 2464, 2526
AsuC2I	CCSGG	1 cut(s)	791
AsuHPI	GGTGA	7 cut(s)	46, 199, 688, 1489, 2090, 2636, 2680
AsuII	TTCGAA	1 cut(s)	1178
AvaI	CYCGRG	6 cut(s)	492, 932, 1302, 2150, 2319, 2528
AxyI	CCTNAGG	1 cut(s)	2654
BaeGI	GKGCMC	1 cut(s)	2467
BanI	GGYRCC	1 cut(s)	282
BanII	GRGCYC	1 cut(s)	2467
BarI	GAAGNNNNNNTAC	2 cut(s)	2838, 2870
BbvCI	CCTCAGC	1 cut(s)	2090
BcgI	CGANNNNNNTGC	4 cut(s)	473, 507, 680, 714
BciT130I	CCWGG	5 cut(s)	775, 2282, 2468, 2717, 3156
BclI	TGATCA	2 cut(s)	442, 1252
BcnI	CCSGG	1 cut(s)	791
BcoDI	GTCTC	6 cut(s)	72, 174, 720, 986, 1923, 2441
BfaI	CTAG	5 cut(s)	411, 1638, 2234, 2516, 3086
BfmI	CTRYAG	3 cut(s)	848, 975, 1567
BfoI	RGCGCY	1 cut(s)	1394
BfrI	CTTAAG	2 cut(s)	2032, 2647
BglII	AGATCT	1 cut(s)	1793
Bme1390I	CCNGG	6 cut(s)	775, 791, 2282, 2468, 2717, 3156
BmeT110I	CYCGRG	6 cut(s)	492, 932, 1302, 2150, 2319, 2528
BmgBI	CACGTC	3 cut(s)	82, 1400, 2818
BmgT120I	GGNCC	3 cut(s)	2463, 2464, 2526
BmiI	GGNNCC	2 cut(s)	284, 2465
BmrFI	CCNGG	6 cut(s)	775, 791, 2282, 2468, 2717, 3156
BmrI	ACTGGG	2 cut(s)	1513, 2951
BmuI	ACTGGG	2 cut(s)	1513, 2951
BplI	GAGNNNNNCTC	4 cut(s)	350, 382, 2144, 2176
Bpu10I	CCTNAGC	3 cut(s)	969, 2090, 3338
Bpu14I	TTCGAA	1 cut(s)	1178
BpuEI	CTTGAG	2 cut(s)	122, 938
BpuMI	CCSGG	1 cut(s)	791
Bsa29I	ATCGAT	3 cut(s)	30, 255, 1065
BsaAI	YACGTR	1 cut(s)	902
BsaBI	GATNNNNATC	2 cut(s)	1243, 2631
BsaHI	GRCGYC	2 cut(s)	2352, 2434
BsaJI	CCNNGG	9 cut(s)	295, 789, 1105, 2151, 2216, 2466, 2467, 2715, 2733
BsaWI	WCCGGW	1 cut(s)	1137
BsaXI	ACNNNNNCTCC	6 cut(s)	171, 201, 1877, 1907, 2578, 2608
Bsc4I	CCNNNNNNNGG	7 cut(s)	207, 496, 1306, 1474, 2222, 2365, 3201
Bse1I	ACTGG	2 cut(s)	1519, 2946
Bse21I	CCTNAGG	1 cut(s)	2654
Bse8I	GATNNNNATC	2 cut(s)	1243, 2631
BseBI	CCWGG	5 cut(s)	775, 2282, 2468, 2717, 3156
BseCI	ATCGAT	3 cut(s)	30, 255, 1065
BseDI	CCNNGG	9 cut(s)	295, 789, 1105, 2151, 2216, 2466, 2467, 2715, 2733
BseGI	GGATG	8 cut(s)	123, 148, 361, 939, 1171, 1618, 2596, 2876
BseJI	GATNNNNATC	2 cut(s)	1243, 2631
BseLI	CCNNNNNNNGG	7 cut(s)	207, 496, 1306, 1474, 2222, 2365, 3201
BseMII	CTCAG	7 cut(s)	118, 731, 734, 1535, 2104, 2175, 2645
BseNI	ACTGG	2 cut(s)	1519, 2946
BseRI	GAGGAG	2 cut(s)	1631, 2233
BseSI	GKGCMC	1 cut(s)	2467
BsgI	GTGCAG	1 cut(s)	3314
Bsh1236I	CGCG	1 cut(s)	297
BshFI	GGCC	5 cut(s)	773, 2465, 2527, 2792, 2973
BshNI	GGYRCC	1 cut(s)	282
BshVI	ATCGAT	3 cut(s)	30, 255, 1065
BsiHKCI	CYCGRG	6 cut(s)	492, 932, 1302, 2150, 2319, 2528
BsiSI	CCGG	2 cut(s)	791, 1138
BslFI	GGGAC	3 cut(s)	1501, 1606, 2439
BslI	CCNNNNNNNGG	7 cut(s)	207, 496, 1306, 1474, 2222, 2365, 3201
BsmAI	GTCTC	6 cut(s)	72, 174, 720, 986, 1923, 2441
BsmBI	CGTCTC	2 cut(s)	72, 2441
BsmFI	GGGAC	3 cut(s)	1501, 1606, 2439
BsmI	GAATGC	1 cut(s)	3283
BsnI	GGCC	5 cut(s)	773, 2465, 2527, 2792, 2973
BsoBI	CYCGRG	6 cut(s)	492, 932, 1302, 2150, 2319, 2528
Bsp119I	TTCGAA	1 cut(s)	1178
Bsp120I	GGGCCC	1 cut(s)	2463
Bsp1286I	GDGCHC	1 cut(s)	2467
Bsp19I	CCATGG	1 cut(s)	1105
BspACI	CCGC	4 cut(s)	295, 297, 1740, 2779
BspANI	GGCC	5 cut(s)	773, 2465, 2527, 2792, 2973
BspCNI	CTCAG	7 cut(s)	117, 730, 735, 1536, 2103, 2174, 2646
BspDI	ATCGAT	3 cut(s)	30, 255, 1065
BspFNI	CGCG	1 cut(s)	297
BspHI	TCATGA	1 cut(s)	2335
BspLI	GGNNCC	2 cut(s)	284, 2465
BspMAI	CTGCAG	2 cut(s)	852, 979
BspPI	GGATC	5 cut(s)	744, 1545, 2201, 2727, 2930
BspT104I	TTCGAA	1 cut(s)	1178
BspT107I	GGYRCC	1 cut(s)	282
BspTI	CTTAAG	2 cut(s)	2032, 2647
BsrI	ACTGG	2 cut(s)	1519, 2946
BssECI	CCNNGG	9 cut(s)	295, 789, 1105, 2151, 2216, 2466, 2467, 2715, 2733
BssNI	GRCGYC	2 cut(s)	2352, 2434
BssT1I	CCWWGG	2 cut(s)	1105, 2733
Bst2UI	CCWGG	5 cut(s)	775, 2282, 2468, 2717, 3156
Bst6I	CTCTTC	3 cut(s)	1896, 1905, 2232
BstACI	GRCGYC	2 cut(s)	2352, 2434
BstAFI	CTTAAG	2 cut(s)	2032, 2647
BstAPI	GCANNNNNTGC	1 cut(s)	1828
BstBAI	YACGTR	1 cut(s)	902
BstBI	TTCGAA	1 cut(s)	1178
BstC8I	GCNNGC	1 cut(s)	87
BstDSI	CCRYGG	2 cut(s)	295, 1105
BstENI	CCTNNNNNAGG	1 cut(s)	2363
BstF5I	GGATG	8 cut(s)	123, 148, 361, 939, 1171, 1618, 2596, 2876
BstFNI	CGCG	1 cut(s)	297
BstH2I	RGCGCY	1 cut(s)	1394
BstHHI	GCGC	2 cut(s)	593, 1393
BstMAI	GTCTC	6 cut(s)	72, 174, 720, 986, 1923, 2441
BstNI	CCWGG	5 cut(s)	775, 2282, 2468, 2717, 3156
BstNSI	RCATGY	1 cut(s)	1721
BstSCI	CCNGG	6 cut(s)	773, 789, 2280, 2466, 2715, 3154
BstSFI	CTRYAG	3 cut(s)	848, 975, 1567
BstSLI	GKGCMC	1 cut(s)	2467
BstUI	CGCG	1 cut(s)	297
BstX2I	RGATCY	4 cut(s)	736, 1537, 1793, 2719
BstYI	RGATCY	4 cut(s)	736, 1537, 1793, 2719
Bsu15I	ATCGAT	3 cut(s)	30, 255, 1065
Bsu36I	CCTNAGG	1 cut(s)	2654
BsuRI	GGCC	5 cut(s)	773, 2465, 2527, 2792, 2973
BsuTUI	ATCGAT	3 cut(s)	30, 255, 1065
BtgI	CCRYGG	2 cut(s)	295, 1105
BtrI	CACGTC	3 cut(s)	82, 1400, 2818
BtsCI	GGATG	8 cut(s)	123, 148, 361, 939, 1171, 1618, 2596, 2876
BtsI	GCAGTG	2 cut(s)	466, 1276
BtsIMutI	CAGTG	7 cut(s)	202, 466, 618, 717, 1276, 1419, 1518
Cac8I	GCNNGC	1 cut(s)	87
CaiI	CAGNNNCTG	1 cut(s)	1828
CciI	TCATGA	1 cut(s)	2335
CfoI	GCGC	2 cut(s)	593, 1393
Cfr13I	GGNCC	3 cut(s)	2463, 2464, 2526
Cfr42I	CCGCGG	1 cut(s)	298
ClaI	ATCGAT	3 cut(s)	30, 255, 1065
CseI	GACGC	1 cut(s)	158
Csp6I	GTAC	4 cut(s)	724, 1525, 1663, 2644
CspCI	CAANNNNNGTGG	1 cut(s)	3332
CviQI	GTAC	4 cut(s)	724, 1525, 1663, 2644
DraI	TTTAAA	1 cut(s)	2419
DrdI	GACNNNNNNGTC	1 cut(s)	1288
DseDI	GACNNNNNNGTC	1 cut(s)	1288
Eam1104I	CTCTTC	3 cut(s)	1896, 1905, 2232
EarI	CTCTTC	3 cut(s)	1896, 1905, 2232
EciI	GGCGGA	1 cut(s)	1755
Eco130I	CCWWGG	2 cut(s)	1105, 2733
Eco147I	AGGCCT	1 cut(s)	2973
Eco24I	GRGCYC	1 cut(s)	2467
Eco32I	GATATC	1 cut(s)	1410
Eco57I	CTGAAG	5 cut(s)	111, 356, 543, 1920, 2220
Eco81I	CCTNAGG	1 cut(s)	2654
Eco88I	CYCGRG	6 cut(s)	492, 932, 1302, 2150, 2319, 2528
EcoNI	CCTNNNNNAGG	1 cut(s)	2363
EcoO109I	RGGNCCY	1 cut(s)	2464
EcoRII	CCWGG	5 cut(s)	773, 2280, 2466, 2715, 3154
EcoRV	GATATC	1 cut(s)	1410
EcoT14I	CCWWGG	2 cut(s)	1105, 2733
EcoT22I	ATGCAT	1 cut(s)	3283
EcoT38I	GRGCYC	1 cut(s)	2467
ErhI	CCWWGG	2 cut(s)	1105, 2733
Esp3I	CGTCTC	2 cut(s)	72, 2441
FalI	AAGNNNNNCTT	4 cut(s)	54, 86, 853, 885
FaqI	GGGAC	3 cut(s)	1501, 1606, 2439
FauNDI	CATATG	1 cut(s)	3283
FbaI	TGATCA	2 cut(s)	442, 1252
FblI	GTMKAC	1 cut(s)	2411
FokI	GGATG	8 cut(s)	110, 155, 368, 926, 1178, 1625, 2603, 2883
FriOI	GRGCYC	1 cut(s)	2467
FspBI	CTAG	5 cut(s)	411, 1638, 2234, 2516, 3086
GlaI	GCGC	2 cut(s)	592, 1392
HaeII	RGCGCY	1 cut(s)	1394
HaeIII	GGCC	5 cut(s)	773, 2465, 2527, 2792, 2973
HapII	CCGG	2 cut(s)	791, 1138
HgaI	GACGC	1 cut(s)	158
HhaI	GCGC	2 cut(s)	593, 1393
Hin1I	GRCGYC	2 cut(s)	2352, 2434
Hin6I	GCGC	2 cut(s)	591, 1391
HinP1I	GCGC	2 cut(s)	591, 1391
HincII	GTYRAC	3 cut(s)	1186, 1986, 2412
HindII	GTYRAC	3 cut(s)	1186, 1986, 2412
HindIII	AAGCTT	3 cut(s)	2380, 2765, 2880
HpaII	CCGG	2 cut(s)	791, 1138
HphI	GGTGA	7 cut(s)	46, 199, 688, 1489, 2090, 2636, 2680
Hpy166II	GTNNAC	5 cut(s)	316, 1186, 1986, 2302, 2412
Hpy8I	GTNNAC	5 cut(s)	316, 1186, 1986, 2302, 2412
Hpy99I	CGWCG	5 cut(s)	1007, 1294, 2354, 2436, 2819
HpyCH4IV	ACGT	8 cut(s)	81, 722, 901, 1399, 2352, 2434, 2642, 2817
HpySE526I	ACGT	8 cut(s)	81, 722, 901, 1399, 2352, 2434, 2642, 2817
Hsp92I	GRCGYC	2 cut(s)	2352, 2434
HspAI	GCGC	2 cut(s)	591, 1391
Ksp22I	TGATCA	2 cut(s)	442, 1252
KspI	CCGCGG	1 cut(s)	298
LmnI	GCTCC	6 cut(s)	1083, 1388, 1582, 2263, 2599, 3334
MaeI	CTAG	5 cut(s)	411, 1638, 2234, 2516, 3086
MaeII	ACGT	8 cut(s)	81, 722, 901, 1399, 2352, 2434, 2642, 2817
MaeIII	GTNAC	4 cut(s)	52, 187, 274, 2801
MfeI	CAATTG	2 cut(s)	438, 1959
MflI	RGATCY	4 cut(s)	736, 1537, 1793, 2719
MhlI	GDGCHC	1 cut(s)	2467
MlyI	GAGTC	5 cut(s)	1276, 1628, 2417, 2540, 2678
MmeI	TCCRAC	3 cut(s)	1887, 2224, 3140
Mph1103I	ATGCAT	1 cut(s)	3283
MroXI	GAANNNNTTC	1 cut(s)	3114
MslI	CAYNNNNRTG	1 cut(s)	1670
MspA1I	CMGCKG	2 cut(s)	297, 3298
MspCI	CTTAAG	2 cut(s)	2032, 2647
MspI	CCGG	2 cut(s)	791, 1138
MspR9I	CCNGG	6 cut(s)	775, 791, 2282, 2468, 2717, 3156
MunI	CAATTG	2 cut(s)	438, 1959
Mva1269I	GAATGC	1 cut(s)	3283
MvaI	CCWGG	5 cut(s)	775, 2282, 2468, 2717, 3156
MvnI	CGCG	1 cut(s)	297
NciI	CCSGG	1 cut(s)	791
NcoI	CCATGG	1 cut(s)	1105
NdeI	CATATG	1 cut(s)	3283
NlaIV	GGNNCC	2 cut(s)	284, 2465
NmuCI	GTSAC	4 cut(s)	52, 187, 274, 2801
NsiI	ATGCAT	1 cut(s)	3283
NspI	RCATGY	1 cut(s)	1721
NspV	TTCGAA	1 cut(s)	1178
PaeR7I	CTCGAG	4 cut(s)	492, 932, 1302, 2319
PagI	TCATGA	1 cut(s)	2335
PasI	CCCWGGG	1 cut(s)	2467
PceI	AGGCCT	1 cut(s)	2973
PcsI	WCGNNNNNNNCGW	1 cut(s)	687
PctI	GAATGC	1 cut(s)	3283
PdmI	GAANNNNTTC	1 cut(s)	3114
PflMI	CCANNNNNTGG	2 cut(s)	207, 3201
PleI	GAGTC	5 cut(s)	1276, 1627, 2416, 2539, 2678
PpsI	GAGTC	5 cut(s)	1276, 1627, 2416, 2539, 2678
Ppu21I	YACGTR	1 cut(s)	902
Psp6I	CCWGG	5 cut(s)	773, 2280, 2466, 2715, 3154
PspGI	CCWGG	5 cut(s)	773, 2280, 2466, 2715, 3154
PspN4I	GGNNCC	2 cut(s)	284, 2465
PspOMI	GGGCCC	1 cut(s)	2463
PspPI	GGNCC	3 cut(s)	2463, 2464, 2526
PspXI	VCTCGAGB	3 cut(s)	492, 1302, 2319
PsrI	GAACNNNNNNTAC	2 cut(s)	1707, 1739
PstI	CTGCAG	2 cut(s)	852, 979
PstNI	CAGNNNCTG	1 cut(s)	1828
PsuI	RGATCY	4 cut(s)	736, 1537, 1793, 2719
PvuII	CAGCTG	1 cut(s)	3298
RsaI	GTAC	4 cut(s)	725, 1526, 1664, 2645
RsaNI	GTAC	4 cut(s)	724, 1525, 1663, 2644
RseI	CAYNNNNRTG	1 cut(s)	1670
SacII	CCGCGG	1 cut(s)	298
SalI	GTCGAC	1 cut(s)	2410
Sau96I	GGNCC	3 cut(s)	2463, 2464, 2526
SchI	GAGTC	5 cut(s)	1276, 1628, 2417, 2540, 2678
ScrFI	CCNGG	6 cut(s)	775, 791, 2282, 2468, 2717, 3156
SduI	GDGCHC	1 cut(s)	2467
SfcI	CTRYAG	3 cut(s)	848, 975, 1567
Sfr274I	CTCGAG	4 cut(s)	492, 932, 1302, 2319
Sfr303I	CCGCGG	1 cut(s)	298
SfuI	TTCGAA	1 cut(s)	1178
SgrBI	CCGCGG	1 cut(s)	298
SlaI	CTCGAG	4 cut(s)	492, 932, 1302, 2319
SmiMI	CAYNNNNRTG	1 cut(s)	1670
SmlI	CTYRAG	8 cut(s)	137, 492, 932, 953, 1302, 2032, 2319, 2647
SmoI	CTYRAG	8 cut(s)	137, 492, 932, 953, 1302, 2032, 2319, 2647
SseBI	AGGCCT	1 cut(s)	2973
SsiI	CCGC	4 cut(s)	295, 297, 1740, 2779
SspI	AATATT	1 cut(s)	1073
SspMI	CTAG	5 cut(s)	411, 1638, 2234, 2516, 3086
StuI	AGGCCT	1 cut(s)	2973
StyD4I	CCNGG	6 cut(s)	773, 789, 2280, 2466, 2715, 3154
StyI	CCWWGG	2 cut(s)	1105, 2733
TaiI	ACGT	8 cut(s)	84, 725, 904, 1402, 2355, 2437, 2645, 2820
TauI	GCSGC	1 cut(s)	2782
TscAI	CASTG	7 cut(s)	202, 466, 618, 717, 1276, 1419, 1518
TseFI	GTSAC	4 cut(s)	52, 187, 274, 2801
Tsp45I	GTSAC	4 cut(s)	52, 187, 274, 2801
TspGWI	ACGGA	5 cut(s)	1777, 1957, 2488, 2603, 2977
TspRI	CASTG	7 cut(s)	202, 466, 618, 717, 1276, 1419, 1518
Van91I	CCANNNNNTGG	2 cut(s)	207, 3201
Vha464I	CTTAAG	2 cut(s)	2032, 2647
XagI	CCTNNNNNAGG	1 cut(s)	2363
XapI	RAATTY	1 cut(s)	3110
XbaI	TCTAGA	2 cut(s)	1637, 3085
XceI	RCATGY	1 cut(s)	1721
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1842
XhoI	CTCGAG	4 cut(s)	492, 932, 1302, 2319
XmiI	GTMKAC	1 cut(s)	2411
XmnI	GAANNNNTTC	1 cut(s)	3114
XspI	CTAG	5 cut(s)	411, 1638, 2234, 2516, 3086
ZraI	GACGTC	2 cut(s)	2353, 2435
Zsp2I	ATGCAT	1 cut(s)	3283

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.