Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr1

Orthogroup ID

OG0003960

Physical Location & Seq

Genomic Coordinates Forward (+)

18422437 .. 18426089

Gene Structure 3653 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom01g19900.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3420 bp

ATGAGCCAATCGATGACTTATCACATTGGAGTAGGATTCTCTCCTTTCTTTTTTTCCCTTTCTTTCCCTCCCCTTCAATTTGAACGGTCATGGTTAAAGGATGAGATAGAAGGGGATGGAAAAAGGAGGGAAGAGAATTCAGTCCTATCACATCACTTTCATATCGGATTCAAAATATGGAGATGTTACACATCTTCACATCTTAGTAATTCATCATTAAACACAACTATAGGATGTGAGGATCTCTATGATTCCCACAGCTGGATCCGGAGAGGATCCTCTTCCGTCTGCAACCTAACCAGAAATACTGCACACAGAATTCTAAAATGTTCAAAATTTCATATAATTGAGCAAATATTCATCAATTATAGATGCTTTGTCTGCCAGAAAAGGGCAGGATACAAAATGGCAGAACAGATTGTAAGCTTTGTGATCGACAAGTTAATTCAACTACTCATCACCACAGAAGCAAACCTTTCGAGAAACGCACGTACTGAAGTTGGTTTCATCAAAGATGAACTCGAGAGCATCCGATCATTCCTCAAGGAAGCAGATGCTAAGGCTGCAGCAGCAGATGGGGAGACGGGGAATGACGGTGTCAAAACTTGGGTCAATCAAGTAAGAGAAGCAGCTTTTTACATTGAAGATGTCATTGATGAATATTTGCTCCACATCATACAGCATCGTCAACACCATGGATTCATTCGTTTTTTCCATAAAATAATCCGGTTGGTTAAAAAAAAGAGATCACAGGATGGGATAGTTTTGAAAATTGACTGGATAAAAAAATTGGTTCGTGAAATCAAGGATAGAAGTAAAAGATATGGATTCAAATCCATTAATCAGCGTGGCCAGAGCAGTGGAGAAGAGACTCTACCGTGGAACGACCCTCGAGTGGCTTCCCTTTTCATTGAGGAAGCTGAAATTGTAGGGATTGAATCTGCCAGAGATGAGCTAACTCGTTTGTTGGTAGAAGGAGCACCGAGACGTGAAGTGATATCAGTGGTTGGCATGGGAGGACTTGGGAAAACCACTCTTGCCAAGAAAGTATATGATCAGCCGAAGGTGATGGCACACTTTGATTGTTATGCTTGGATTACGGTGTCCCAGTCGTACAGGGTTGAGGATCTTCTGAGGACGGTAATAAAAAAGTTCTACAGTTCAAGGAAGGAGCGTTTCCCAGAGGAAATTGACAAAATGGATGAGGAGTCCCTGATTAGCACATCAAGAGAGTATTTGGAGCAAAAGAGGTACGTTGTTGTGTTTGATGATGTGTGGAAAGTAGATTTTTGTGGGGCTATAGAACATGCTTTACCCGATAAAGGCGGACGAATAATTATCACTACGCAGATTCAAGATGTTGCCAACTTTTGCAAAAGATCTTGTTTTGTTCATGTTCATCACTTGCAGCCTCTGACTCCAAACAAGGCATGGGAATTGTTTTGTAGGAAGGCATTTCGGTTTGAATTGGAGGGAAAGTGTCCTGAAGAGTTGGAAGATTTGTCTCTTAACATCGTCAAAAGATGCGAAGGACTACCATTGGCAATTGTTTCCATCGGTGGTCTGCTGTCAACCAAAGCTAAAGTTGTGTCTGAATGGCAGAAGTTATACAATAGCTTAAGTTCAGAGTTAGAAAGAAATCCCCACCTTACAAGCCTCACAAGAATCCTATCCCTCAGCTATCACCATCTACCGTATTACCTCAAATCGTGTGTATTATGCTTTGGCATCTTTCCCGAGGATTACTCAGTTAGTTGCATAAGATTAGTTCGGTTGTGGATCGATGAAGGCTTTGTGAAACCGAAGAGGGGCAAGACACTAGAAGAGGTTGGAGAGGAATACTTGATGGAGCTAATTCACAGAAGCCTGGTTCAAGTTTCAGAAGTTTACATAGACGGAAAAGCTCGAGGTTGTAGAGTTCATGATCTATTGCATGACGTCCTCCTTAAAAAGGGGTTGGATTCGAGCTTCTGCCATGTGTTATCAAAAGACGAGTCGACTTTTAAACCAACAACTCGACGTCTCTCAATGGACATCAGTCCCCTTGAGGCCCTGGGAAGCATTACACAATCTCATATTCGTTCCGTATTTACTTTCGACCTAGAAAAATGGTCCAAGTCTTTTCTCGATACATTGAGTGGAAACATTAAACTTCTGAAAGTATTGGATTTCACGGATGCTCCAATCAATCAACTTCCGAAATATGTGGGGGATTTGTATCTCTTGACGTATTTAAGCCTGAGGAATACAAAGGTGAAGTTGCTTCCAGACTCCATAGGCAATCTACAAAACTTGGAAACCCTGGATCTAAAACAGTCTCTGGTGTATGAAATACCAGCCAAGATCAGTAAGCTTGTTAAGCTGAGGCATCTTTTGGCCTACCATCGTGACTACAACTACGACGTGGGCTTGTTCTTGAATATGGAAAAAGGAGTGAAGATACATGAAGGTATTGGATGCTTACAAGCTTTGCAAAAACTGTGCTATGTGGAGGCGAATCATGGAGGGATCAGGCTAATCAAAGAACTGGGAAAGTTAAGACAGCTGCGGAAGTTAGGCCTAAAAAACTTGAAAAGTGAAGATGGAAGGGCTGTGTGTGCTTCTATTGAAAAGATGAACCACCTTGAATCTCTAATTTTAACTGCAGTAAATGAAGATGAAGTCCTTGATCTAGAATCCATTTCAACTCCACCACAATTTATCCGTGCTCTCCGCTTGATAGGGCGTTTAGAGCAGTTGCCAGGTTGGATTTCAAATCTTCAACACCTAGTCAGGATAACCATTTTTTGGTCAAGGTTGAGAGATTCCCCACTAAAAGCCCTTCAAAATCTACCTAATCTGTTGGAGCTAGTTTTGGCTATCAATGCCTATGATGGTGTGCAGCTGCATTTTGAGGAAGAAGGATTTCAGAAACTTAGAGTGCTAGGACTCAGGGAAATGGAGGGACTAAATTCGTTGATCATAGACGATGGGGTCATGCCTCTTCTGCAAGAGTTTCACGTTGGACCTTCCCCACAACTGAAGGAGGTGCCCTCTGGCATCCACCATCTCCGGAATCTGACATATCTTAATTTCTGTGACATGCCAAAGGAGTTTACGCGGCAGATGGATCCAAAAAATGGCCAACATTATTGGATTGTTGAACATATAAAAAATGTTAGTTTTAGTTACAAGGTCAGACCAAGATGGGGTGCATTTGAAAGTCATGCCCTGCGTGATTCCAGCTTTTTCTCTTTGGTGGAATGCTTGATTCCAACTTGTGAAACCAAGCATCGGAATGGGACATGTTTTGGTTTAAGAGTCGGTTGTTACTCTTTCTCTTCTGTTTTCTTTGTGTCGTTCAGTCGTATTCCTAGCATTTTCCTTGTAATTTTATTGGTGGTTGCAATAAAAATATCCTATCCAATTGATTTTCTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0002376 GO:0003674 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0008219 GO:0009626 GO:0009987 GO:0012501 GO:0016020 GO:0019897 GO:0019898 GO:0023052 GO:0033554 GO:0034050 GO:0036094 GO:0044425 GO:0044459 GO:0044464 GO:0045087 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071944 GO:0097159 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko04626 map04626

Pfam Domains

Protein Families

NB-ARC

Protein Analysis

1140

Amino Acids

130.95

Weight (kDa)

8.61

Isoelectric Point (pI)

47.18

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003960)

Species	Orthologous Gene IDs
malus_domestica	MD00G1097800.v1.1 MD01G1186500.v1.1 MD01G1186600.v1.1 MD01G1186800.v1.1 MD01G1187000.v1.1 MD01G1187100.v1.1 MD07G1258600.v1.1 MD07G1258700.v1.1 MD07G1258800.v1.1 MD07G1258900.v1.1 MD07G1259200.v1.1 MD07G1259300.v1.1
prunus_persica	Prupe.2G283200_v2.0.a1 Prupe.2G283300_v2.0.a1 Prupe.2G283400_v2.0.a1
pyrus_communis	pycom01g19810 pycom01g19820 pycom01g19840 pycom01g19900 pycom07g23170 pycom07g23230 pycom07g23240 pycom07g23250 pycom07g23260
rosa_chinensis	RchiOBHm_Chr1g0375241
rosa_laevigata	RLG00000026676
rosa_multiflora	Rmu_co8330553.1_g000001 Rmu_sc0000554.1_g000032
rosa_roxburghii	Rroxscaffold_4G00282910
rosa_rugosa	Rorug01G0389400
rosa_samantha	Rh1AG400100 Rh1BG364400 Rh1DG395000
rosa_wichuraiana	Rw1G035520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2972
AatII	GACGTC	2 cut(s)	1941, 2023
AccB1I	GGYRCC	1 cut(s)	3028
AccB7I	CCANNNNNTGG	2 cut(s)	2787, 3119
AccI	GTMKAC	1 cut(s)	1997
AccII	CGCG	1 cut(s)	3100
AccIII	TCCGGA	2 cut(s)	267, 3051
AciI	CCGC	4 cut(s)	1326, 2548, 2713, 3100
AcoI	YGGCCR	2 cut(s)	850, 3121
AcsI	RAATTY	4 cut(s)	136, 318, 335, 2950
AcuI	CTGAAG	3 cut(s)	516, 1506, 3041
AcyI	GRCGYC	2 cut(s)	1938, 2020
AfaI	GTAC	3 cut(s)	493, 1115, 1253
AflII	CTTAAG	1 cut(s)	1618
AflIII	ACRYGT	1 cut(s)	3284
AjiI	CACGTC	2 cut(s)	989, 2404
AjnI	CCWGG	4 cut(s)	1866, 2052, 2301, 2740
Alw21I	GWGCWC	2 cut(s)	982, 2710
Alw26I	GTCTC	6 cut(s)	575, 863, 979, 1509, 2027, 2322
AlwNI	CAGNNNCTG	2 cut(s)	1414, 2320
Ama87I	CYCGRG	4 cut(s)	521, 891, 1736, 1905
Aor13HI	TCCGGA	2 cut(s)	267, 3051
AoxI	GGCC	5 cut(s)	850, 2049, 2376, 2557, 3121
ApeKI	GCWGC	8 cut(s)	563, 566, 569, 629, 1408, 2545, 2881, 2884
ApoI	RAATTY	4 cut(s)	136, 318, 335, 2950
ArsI	GACNNNNNNTTYG	4 cut(s)	594, 626, 1979, 2011
AseI	ATTAAT	1 cut(s)	840
Asp700I	GAANNNNTTC	1 cut(s)	2904
AspS9I	GGNCC	3 cut(s)	2050, 2112, 3005
AsuHPI	GGTGA	4 cut(s)	451, 1078, 1676, 2266
AvaI	CYCGRG	4 cut(s)	521, 891, 1736, 1905
AvaII	GGWCC	2 cut(s)	2112, 3005
AxyI	CCTNAGG	1 cut(s)	2240
BaeGI	GKGCMC	1 cut(s)	3033
BalI	TGGCCA	2 cut(s)	852, 3123
BamHI	GGATCC	3 cut(s)	264, 275, 3109
BanI	GGYRCC	1 cut(s)	3028
BarI	GAAGNNNNNNTAC	2 cut(s)	858, 890
Bbv12I	GWGCWC	2 cut(s)	982, 2710
BbvCI	CCTCAGC	2 cut(s)	1676, 2363
BbvI	GCAGC	8 cut(s)	550, 578, 581, 641, 1420, 2532, 2871, 2893
BcgI	CGANNNNNNTGC	2 cut(s)	459, 493
BciT130I	CCWGG	4 cut(s)	1868, 2054, 2303, 2742
BciVI	GTATCC	1 cut(s)	392
BclI	TGATCA	2 cut(s)	1054, 2958
BcoDI	GTCTC	6 cut(s)	575, 863, 979, 1509, 2027, 2322
BfaI	CTAG	8 cut(s)	1820, 2102, 2672, 2768, 2849, 2924, 3354, 3418
BfmI	CTRYAG	5 cut(s)	228, 564, 1156, 1299, 2643
BfrI	CTTAAG	1 cut(s)	1618
BfuI	GTATCC	1 cut(s)	392
BglII	AGATCT	1 cut(s)	1379
BisI	GCNGC	9 cut(s)	564, 567, 570, 630, 1409, 2546, 2882, 2885, 3101
BlsI	GCNGC	9 cut(s)	565, 568, 571, 631, 1410, 2547, 2883, 2886, 3102
Bme1390I	CCNGG	4 cut(s)	1868, 2054, 2303, 2742
Bme18I	GGWCC	2 cut(s)	2112, 3005
BmeT110I	CYCGRG	4 cut(s)	521, 891, 1736, 1905
BmgBI	CACGTC	2 cut(s)	989, 2404
BmgT120I	GGNCC	3 cut(s)	2050, 2112, 3005
BmiI	GGNNCC	4 cut(s)	266, 277, 3030, 3111
BmrFI	CCNGG	4 cut(s)	1868, 2054, 2303, 2742
BmrI	ACTGGG	2 cut(s)	1102, 2537
BmuI	ACTGGG	2 cut(s)	1102, 2537
BoxI	GACNNNNGTC	1 cut(s)	2037
BplI	GAGNNNNNCTC	4 cut(s)	1730, 1762, 3017, 3049
Bpu10I	CCTNAGC	3 cut(s)	558, 1676, 2363
BpuEI	CTTGAG	2 cut(s)	527, 2066
Bsa29I	ATCGAT	2 cut(s)	11, 1782
BsaAI	YACGTR	1 cut(s)	491
BsaBI	GATNNNNATC	2 cut(s)	832, 2217
BsaHI	GRCGYC	2 cut(s)	1938, 2020
BsaJI	CCNNGG	6 cut(s)	694, 878, 1737, 2052, 2053, 2301
BsaWI	WCCGGW	3 cut(s)	267, 726, 3051
BsaXI	ACNNNNNCTCC	4 cut(s)	1463, 1493, 2164, 2194
Bse1I	ACTGG	3 cut(s)	782, 1108, 2532
Bse21I	CCTNAGG	1 cut(s)	2240
Bse8I	GATNNNNATC	2 cut(s)	832, 2217
BseAI	TCCGGA	2 cut(s)	267, 3051
BseBI	CCWGG	4 cut(s)	1868, 2054, 2303, 2742
BseCI	ATCGAT	2 cut(s)	11, 1782
BseDI	CCNNGG	6 cut(s)	694, 878, 1737, 2052, 2053, 2301
BseGI	GGATG	9 cut(s)	106, 121, 239, 528, 760, 1207, 2182, 2462, 3039
BseJI	GATNNNNATC	2 cut(s)	832, 2217
BseMII	CTCAG	6 cut(s)	1124, 1690, 1761, 2231, 2354, 2944
BseNI	ACTGG	3 cut(s)	782, 1108, 2532
BseRI	GAGGAG	1 cut(s)	1220
BseSI	GKGCMC	1 cut(s)	3033
BseXI	GCAGC	8 cut(s)	550, 578, 581, 641, 1420, 2532, 2871, 2893
BsgI	GTGCAG	2 cut(s)	294, 2900
Bsh1236I	CGCG	1 cut(s)	3100
BshFI	GGCC	5 cut(s)	852, 2051, 2378, 2559, 3123
BshNI	GGYRCC	1 cut(s)	3028
BshVI	ATCGAT	2 cut(s)	11, 1782
BsiHKAI	GWGCWC	2 cut(s)	982, 2710
BsiHKCI	CYCGRG	4 cut(s)	521, 891, 1736, 1905
BsiSI	CCGG	3 cut(s)	268, 727, 3052
BslFI	GGGAC	5 cut(s)	1090, 1195, 2025, 2958, 3295
BsmAI	GTCTC	6 cut(s)	575, 863, 979, 1509, 2027, 2322
BsmBI	CGTCTC	3 cut(s)	575, 979, 2027
BsmFI	GGGAC	5 cut(s)	1090, 1195, 2025, 2958, 3295
BsmI	GAATGC	1 cut(s)	3248
BsnI	GGCC	5 cut(s)	852, 2051, 2378, 2559, 3123
BsoBI	CYCGRG	4 cut(s)	521, 891, 1736, 1905
Bsp1286I	GDGCHC	3 cut(s)	982, 2710, 3033
Bsp13I	TCCGGA	2 cut(s)	267, 3051
Bsp19I	CCATGG	1 cut(s)	694
BspACI	CCGC	4 cut(s)	1326, 2548, 2713, 3100
BspANI	GGCC	5 cut(s)	852, 2051, 2378, 2559, 3123
BspCNI	CTCAG	6 cut(s)	1125, 1689, 1760, 2232, 2355, 2943
BspDI	ATCGAT	2 cut(s)	11, 1782
BspEI	TCCGGA	2 cut(s)	267, 3051
BspFNI	CGCG	1 cut(s)	3100
BspHI	TCATGA	1 cut(s)	1921
BspLI	GGNNCC	4 cut(s)	266, 277, 3030, 3111
BspMAI	CTGCAG	2 cut(s)	568, 2647
BspT107I	GGYRCC	1 cut(s)	3028
BspTI	CTTAAG	1 cut(s)	1618
BsrI	ACTGG	3 cut(s)	782, 1108, 2532
BssECI	CCNNGG	6 cut(s)	694, 878, 1737, 2052, 2053, 2301
BssNI	GRCGYC	2 cut(s)	1938, 2020
BssT1I	CCWWGG	1 cut(s)	694
Bst2UI	CCWGG	4 cut(s)	1868, 2054, 2303, 2742
Bst4CI	ACNGT	9 cut(s)	87, 596, 879, 1102, 1141, 1160, 1695, 2316, 2481
Bst6I	CTCTTC	8 cut(s)	126, 286, 861, 1482, 1799, 1818, 2988, 3325
BstACI	GRCGYC	2 cut(s)	1938, 2020
BstAFI	CTTAAG	1 cut(s)	1618
BstBAI	YACGTR	1 cut(s)	491
BstDEI	CTNAG	9 cut(s)	203, 558, 1133, 1676, 1747, 2240, 2363, 2915, 2930
BstDSI	CCRYGG	2 cut(s)	694, 878
BstENI	CCTNNNNNAGG	1 cut(s)	1949
BstF5I	GGATG	9 cut(s)	106, 121, 239, 528, 760, 1207, 2182, 2462, 3039
BstFNI	CGCG	1 cut(s)	3100
BstMAI	GTCTC	6 cut(s)	575, 863, 979, 1509, 2027, 2322
BstMWI	GCNNNNNNNGC	8 cut(s)	381, 563, 569, 2359, 2597, 2731, 2864, 2986
BstNI	CCWGG	4 cut(s)	1868, 2054, 2303, 2742
BstNSI	RCATGY	3 cut(s)	1310, 3085, 3288
BstPAI	GACNNNNGTC	1 cut(s)	2037
BstSCI	CCNGG	4 cut(s)	1866, 2052, 2301, 2740
BstSFI	CTRYAG	5 cut(s)	228, 564, 1156, 1299, 2643
BstSLI	GKGCMC	1 cut(s)	3033
BstUI	CGCG	1 cut(s)	3100
BstV1I	GCAGC	8 cut(s)	550, 578, 581, 641, 1420, 2532, 2871, 2893
BstX2I	RGATCY	7 cut(s)	241, 264, 275, 1126, 1379, 2305, 3109
BstXI	CCANNNNNNTGG	1 cut(s)	860
BstYI	RGATCY	7 cut(s)	241, 264, 275, 1126, 1379, 2305, 3109
Bsu15I	ATCGAT	2 cut(s)	11, 1782
Bsu36I	CCTNAGG	1 cut(s)	2240
BsuI	GTATCC	1 cut(s)	392
BsuRI	GGCC	5 cut(s)	852, 2051, 2378, 2559, 3123
BsuTUI	ATCGAT	2 cut(s)	11, 1782
BtgI	CCRYGG	2 cut(s)	694, 878
BtrI	CACGTC	2 cut(s)	989, 2404
BtsCI	GGATG	9 cut(s)	106, 121, 239, 528, 760, 1207, 2182, 2462, 3039
BtsI	GCAGTG	1 cut(s)	865
BtsIMutI	CAGTG	2 cut(s)	865, 1008
CaiI	CAGNNNCTG	2 cut(s)	1414, 2320
CciI	TCATGA	1 cut(s)	1921
Cfr13I	GGNCC	3 cut(s)	2050, 2112, 3005
ClaI	ATCGAT	2 cut(s)	11, 1782
Csp6I	GTAC	3 cut(s)	492, 1114, 1252
CviQI	GTAC	3 cut(s)	492, 1114, 1252
DdeI	CTNAG	9 cut(s)	203, 558, 1133, 1676, 1747, 2240, 2363, 2915, 2930
DraI	TTTAAA	1 cut(s)	2005
DrdI	GACNNNNNNGTC	1 cut(s)	2972
DseDI	GACNNNNNNGTC	1 cut(s)	2972
EaeI	YGGCCR	2 cut(s)	850, 3121
Eam1104I	CTCTTC	8 cut(s)	126, 286, 861, 1482, 1799, 1818, 2988, 3325
EarI	CTCTTC	8 cut(s)	126, 286, 861, 1482, 1799, 1818, 2988, 3325
EciI	GGCGGA	1 cut(s)	1341
Eco130I	CCWWGG	1 cut(s)	694
Eco147I	AGGCCT	1 cut(s)	2559
Eco32I	GATATC	1 cut(s)	999
Eco47I	GGWCC	2 cut(s)	2112, 3005
Eco57I	CTGAAG	3 cut(s)	516, 1506, 3041
Eco81I	CCTNAGG	1 cut(s)	2240
Eco88I	CYCGRG	4 cut(s)	521, 891, 1736, 1905
EcoNI	CCTNNNNNAGG	1 cut(s)	1949
EcoO109I	RGGNCCY	1 cut(s)	2050
EcoRI	GAATTC	2 cut(s)	136, 318
EcoRII	CCWGG	4 cut(s)	1866, 2052, 2301, 2740
EcoRV	GATATC	1 cut(s)	999
EcoT14I	CCWWGG	1 cut(s)	694
ErhI	CCWWGG	1 cut(s)	694
Esp3I	CGTCTC	3 cut(s)	575, 979, 2027
FalI	AAGNNNNNCTT	2 cut(s)	459, 491
FaqI	GGGAC	5 cut(s)	1090, 1195, 2025, 2958, 3295
FbaI	TGATCA	2 cut(s)	1054, 2958
FblI	GTMKAC	1 cut(s)	1997
Fnu4HI	GCNGC	9 cut(s)	564, 567, 570, 630, 1409, 2546, 2882, 2885, 3101
FokI	GGATG	9 cut(s)	113, 128, 246, 515, 767, 1214, 2189, 2469, 3026
Fsp4HI	GCNGC	9 cut(s)	564, 567, 570, 630, 1409, 2546, 2882, 2885, 3101
FspBI	CTAG	8 cut(s)	1820, 2102, 2672, 2768, 2849, 2924, 3354, 3418
GluI	GCNGC	9 cut(s)	564, 567, 570, 630, 1409, 2546, 2882, 2885, 3101
HaeIII	GGCC	5 cut(s)	852, 2051, 2378, 2559, 3123
HapII	CCGG	3 cut(s)	268, 727, 3052
Hin1I	GRCGYC	2 cut(s)	1938, 2020
HincII	GTYRAC	3 cut(s)	689, 1572, 1998
HindII	GTYRAC	3 cut(s)	689, 1572, 1998
HindIII	AAGCTT	3 cut(s)	424, 2351, 2466
HpaII	CCGG	3 cut(s)	268, 727, 3052
HphI	GGTGA	4 cut(s)	451, 1078, 1676, 2266
Hpy166II	GTNNAC	5 cut(s)	689, 1572, 1888, 1998, 3096
Hpy8I	GTNNAC	5 cut(s)	689, 1572, 1888, 1998, 3096
Hpy99I	CGWCG	2 cut(s)	2022, 2405
HpyCH4III	ACNGT	9 cut(s)	87, 596, 879, 1102, 1141, 1160, 1695, 2316, 2481
HpyCH4IV	ACGT	8 cut(s)	490, 988, 1254, 1938, 2020, 2228, 2403, 3000
HpyF10VI	GCNNNNNNNGC	8 cut(s)	381, 563, 569, 2359, 2597, 2731, 2864, 2986
HpyF3I	CTNAG	9 cut(s)	203, 558, 1133, 1676, 1747, 2240, 2363, 2915, 2930
HpySE526I	ACGT	8 cut(s)	490, 988, 1254, 1938, 2020, 2228, 2403, 3000
Hsp92I	GRCGYC	2 cut(s)	1938, 2020
Kpn2I	TCCGGA	2 cut(s)	267, 3051
Ksp22I	TGATCA	2 cut(s)	1054, 2958
LmnI	GCTCC	7 cut(s)	672, 977, 1171, 1240, 1849, 2185, 2845
Lsp1109I	GCAGC	8 cut(s)	550, 578, 581, 641, 1420, 2532, 2871, 2893
MaeI	CTAG	8 cut(s)	1820, 2102, 2672, 2768, 2849, 2924, 3354, 3418
MaeII	ACGT	8 cut(s)	490, 988, 1254, 1938, 2020, 2228, 2403, 3000
MaeIII	GTNAC	5 cut(s)	185, 2387, 3077, 3167, 3308
MfeI	CAATTG	2 cut(s)	1545, 3405
MflI	RGATCY	7 cut(s)	241, 264, 275, 1126, 1379, 2305, 3109
MhlI	GDGCHC	3 cut(s)	982, 2710, 3033
MlsI	TGGCCA	2 cut(s)	852, 3123
MluNI	TGGCCA	2 cut(s)	852, 3123
MlyI	GAGTC	7 cut(s)	865, 1217, 1411, 2003, 2264, 2922, 3309
MmeI	TCCRAC	7 cut(s)	1473, 1810, 1938, 2726, 2823, 2983, 3278
Mox20I	TGGCCA	2 cut(s)	852, 3123
MroI	TCCGGA	2 cut(s)	267, 3051
MroXI	GAANNNNTTC	1 cut(s)	2904
MscI	TGGCCA	2 cut(s)	852, 3123
MslI	CAYNNNNRTG	1 cut(s)	3276
Msp20I	TGGCCA	2 cut(s)	852, 3123
MspA1I	CMGCKG	3 cut(s)	261, 2545, 2884
MspCI	CTTAAG	1 cut(s)	1618
MspI	CCGG	3 cut(s)	268, 727, 3052
MspR9I	CCNGG	4 cut(s)	1868, 2054, 2303, 2742
MunI	CAATTG	2 cut(s)	1545, 3405
Mva1269I	GAATGC	1 cut(s)	3248
MvaI	CCWGG	4 cut(s)	1868, 2054, 2303, 2742
MvnI	CGCG	1 cut(s)	3100
MwoI	GCNNNNNNNGC	8 cut(s)	381, 563, 569, 2359, 2597, 2731, 2864, 2986
NcoI	CCATGG	1 cut(s)	694
NlaIV	GGNNCC	4 cut(s)	266, 277, 3030, 3111
NmuCI	GTSAC	2 cut(s)	2387, 3077
NspI	RCATGY	3 cut(s)	1310, 3085, 3288
PaeR7I	CTCGAG	3 cut(s)	521, 891, 1905
PagI	TCATGA	1 cut(s)	1921
PasI	CCCWGGG	1 cut(s)	2053
PceI	AGGCCT	1 cut(s)	2559
PciI	ACATGT	1 cut(s)	3284
PctI	GAATGC	1 cut(s)	3248
PdmI	GAANNNNTTC	1 cut(s)	2904
PflFI	GACNNNGTC	1 cut(s)	596
PflMI	CCANNNNNTGG	2 cut(s)	2787, 3119
PkrI	GCNGC	9 cut(s)	565, 568, 571, 631, 1410, 2547, 2883, 2886, 3102
PleI	GAGTC	7 cut(s)	865, 1216, 1411, 2002, 2264, 2922, 3308
PpsI	GAGTC	7 cut(s)	865, 1216, 1411, 2002, 2264, 2922, 3308
Ppu21I	YACGTR	1 cut(s)	491
PscI	ACATGT	1 cut(s)	3284
PshAI	GACNNNNGTC	1 cut(s)	2037
PshBI	ATTAAT	1 cut(s)	840
Psp6I	CCWGG	4 cut(s)	1866, 2052, 2301, 2740
PspGI	CCWGG	4 cut(s)	1866, 2052, 2301, 2740
PspN4I	GGNNCC	4 cut(s)	266, 277, 3030, 3111
PspPI	GGNCC	3 cut(s)	2050, 2112, 3005
PspXI	VCTCGAGB	2 cut(s)	891, 1905
PsrI	GAACNNNNNNTAC	2 cut(s)	1296, 1328
PstI	CTGCAG	2 cut(s)	568, 2647
PstNI	CAGNNNCTG	2 cut(s)	1414, 2320
PsuI	RGATCY	7 cut(s)	241, 264, 275, 1126, 1379, 2305, 3109
PsyI	GACNNNGTC	1 cut(s)	596
PvuII	CAGCTG	3 cut(s)	261, 2545, 2884
RsaI	GTAC	3 cut(s)	493, 1115, 1253
RsaNI	GTAC	3 cut(s)	492, 1114, 1252
RseI	CAYNNNNRTG	1 cut(s)	3276
SalI	GTCGAC	1 cut(s)	1996
SatI	GCNGC	9 cut(s)	564, 567, 570, 630, 1409, 2546, 2882, 2885, 3101
Sau96I	GGNCC	3 cut(s)	2050, 2112, 3005
SchI	GAGTC	7 cut(s)	865, 1217, 1411, 2003, 2264, 2922, 3309
ScrFI	CCNGG	4 cut(s)	1868, 2054, 2303, 2742
SduI	GDGCHC	3 cut(s)	982, 2710, 3033
SfcI	CTRYAG	5 cut(s)	228, 564, 1156, 1299, 2643
Sfr274I	CTCGAG	3 cut(s)	521, 891, 1905
SinI	GGWCC	2 cut(s)	2112, 3005
SlaI	CTCGAG	3 cut(s)	521, 891, 1905
SmiMI	CAYNNNNRTG	1 cut(s)	3276
SmlI	CTYRAG	6 cut(s)	521, 542, 891, 1618, 1905, 2045
SmoI	CTYRAG	6 cut(s)	521, 542, 891, 1618, 1905, 2045
SseBI	AGGCCT	1 cut(s)	2559
SsiI	CCGC	4 cut(s)	1326, 2548, 2713, 3100
SspI	AATATT	2 cut(s)	357, 662
SspMI	CTAG	8 cut(s)	1820, 2102, 2672, 2768, 2849, 2924, 3354, 3418
StuI	AGGCCT	1 cut(s)	2559
StyD4I	CCNGG	4 cut(s)	1866, 2052, 2301, 2740
StyI	CCWWGG	1 cut(s)	694
TaaI	ACNGT	9 cut(s)	87, 596, 879, 1102, 1141, 1160, 1695, 2316, 2481
TaiI	ACGT	8 cut(s)	493, 991, 1257, 1941, 2023, 2231, 2406, 3003
TauI	GCSGC	1 cut(s)	3103
TscAI	CASTG	2 cut(s)	865, 1008
TseFI	GTSAC	2 cut(s)	2387, 3077
TseI	GCWGC	8 cut(s)	563, 566, 569, 629, 1408, 2545, 2881, 2884
Tsp45I	GTSAC	2 cut(s)	2387, 3077
TspGWI	ACGGA	5 cut(s)	274, 1911, 2074, 2189, 2693
TspRI	CASTG	2 cut(s)	865, 1008
Tth111I	GACNNNGTC	1 cut(s)	596
Van91I	CCANNNNNTGG	2 cut(s)	2787, 3119
Vha464I	CTTAAG	1 cut(s)	1618
VpaK11BI	GGWCC	2 cut(s)	2112, 3005
VspI	ATTAAT	1 cut(s)	840
XagI	CCTNNNNNAGG	1 cut(s)	1949
XapI	RAATTY	4 cut(s)	136, 318, 335, 2950
XbaI	TCTAGA	1 cut(s)	2671
XceI	RCATGY	3 cut(s)	1310, 3085, 3288
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1428
XhoI	CTCGAG	3 cut(s)	521, 891, 1905
XmiI	GTMKAC	1 cut(s)	1997
XmnI	GAANNNNTTC	1 cut(s)	2904
XspI	CTAG	8 cut(s)	1820, 2102, 2672, 2768, 2849, 2924, 3354, 3418
ZraI	GACGTC	2 cut(s)	1939, 2021

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.