Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0003960

Physical Location & Seq

Genomic Coordinates Forward (+)

32525935 .. 32528928

Gene Structure 2994 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1259200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2994 bp

ATGCTAAAGCCATATGCTTCACATCATCTTCTTGATATCAGATTTCGTTTCTCTACCCTTGATTCCCAATTTTTTGTGTTTTTCTCCGAGCAACTGTTTGCTCACACAAACCAAACAGCTAGAGTTCAGTGTGGGGTTGTTGGATGCTCATACTATATGGCAGAACAAATTGTATGCTTTGTGATCGAAAAGTTAATTAAACTACTCATCACCACAGAAGCAAACCTTTCAAGAGACGTATGCAAAGAAGTTGGTTTCATCAAAGATGAGCTAGAGAGCATCCGATTATTCCTCAAGGACGCAGATGCTAAGGCGGCAGAAGATGGAGAGACGACAAATGACAGTGTCAAAACTTGGGTCAACCAAGTAAGAGAAGCAGCTTTTTACATTGAAGATGTCATTGATGAATATTTGCTTCGTGTCACAAGGCACCTTCAAGATCGTGGATTTATTCCGCTTTTCCACAAAATTATCCGGTTGGTAAAAAAAATGAGATCACAGGATGGGATAGTTTCGAAAGTTGACCGTATGAAAGAATTGGTTCGTGGTATTCAGGAAAGAAGACAAAGATACGAACTCAGTTCCATTGACCGGCAAGGTCGAGATAATGGCGAAAAGACTCTTCTGTGGTATGACCCTCGAGTGGCTTCCCTTTTCATTGAGGAGGCTGAAGTGGTGGGGATTGAATCTGCCAGAAAAGAGCTAACTGATTTGTTGGTCGGAGGAGCGCCGAGACGCGAAGTGATATCTGTGGTTGGCATGGGAGGACTTGGGAAGACAACTCTTGCCAGGAAAGTTTATGATAGTCTGACGGTGATGGACCACTTTAAATGCTACGCTTGGATTACGGTGTCCCAGTCGTATAGGGTGGAGGATCTTCTGAGGACGGTAGTCAAAATGTTCTACAATTCAAGAAAGGAGAACTTCCCAGAGGACATTGACACAACGGACGAGGAGTCCCTGATCAGAAAATCAAGAGAGTATTTGCAGCAAAAGAGGTACGTTGTTGTGTTTGATGATGTGTGGAAAGTAAAGTTTTGGGAGGCTATAGAACATGCATTACCGGATAATAAAGGCGGCCGAATAATGATTACTACAAGGATTCAAGAAGTTGCGGATTTTTGCAAAAAATCTTGTTTCGTTCATGTCCATCACTTGCAACCTCTGCCTCCGAAGAATGCATGGAAATTGTTTTGTAGAAAGGCATTCCAGTTTGAATTGGAGGGAAATTGCCCTCTAGAGTTAGAAGATATGTCACGGGAAATCGTTAAGAAGTGCGAGGGGCTACCTTTGGCAATTGTTTGCATAGGTGGTCTGCTGTCAACGAAAGTTAAAGTGGTGTCTGAATGGCAGAAGTTATACAATAGCTTAAGTTCAGAGTTGAAAAGCAATCCTCATCTTACAGGCCTCACAAGAATCCTATCCCTCAGCTATGAGCATCTACCATATTACCTCAAATCGTGTGTCTTATACTTTGGCATCTTTCCAGAGGACTACTCAATTAATTGCGTAAGATTGATACGGTTGTGGATTGCTGAAGGGTTTGTAAAATTGAAGAAGGGCAAGACACTGGAAGAGGTTGGAGAGGAATATTTGACTGAGGTAATTCACAGAAGCCTGGTTCAAGTGTCAGAAGTTTACATTGATGGAAAAGCTCGAAGTTGTAGAGTTCATGATATTTTGCGCGAAGTGCTCCTTAAAAAGGGTGTGGATTCAAGCTTCTGTCATATTTTATCAGAATTTGAGTTGACTTATAAACCAACAATTCGACGTCTCTCAATCGACAACAGCCCCTCTGATGCCTCGGGAATCATTACACAGTCTCAAATTCGTTCTGTATTTGCTTTCAACCAAGAAGAATGGCCCGAGTCTTATCTCAATACATTGAGGGGAAACTTTAAACTTCTGAAAGTATTGGATTTCATGGATGCTCCAATCAATCAACTTCCAAAATATGTGGGGGATTTGTATCTCTTGACGTACTTAAGCCTGAGGAATACAAAGGTGGAGTTGCTTCCAGACTCCATAGGCAATCTGCAAAACTTAGAAACCCTGGATCTAAAACAATCTTTGGTGTCTGAAATACCAGCCAAGATCAATAAGCTTGTTAAGCTGCGGCATCTTTTGGCCTACTATCGTGACTACAGCTTGGCGTTTTGGTCGAATATGGAAAGAGGAGTGAAGATACATGAAGGTATTGGATGCTTACAAGCTTTGCAAAAACTGTACTTTGTGGAGGCGAATCATGGAGGGATCAAACTAATCAAAGAACTGGGACAGTTAAGGCAGTTGCGGAAGTTAGGCCTAACAAAGTTAAAACGTGAAGATGGAAAGGCTCTTTGTGCTTGTATTGAAAAGATAAATCACCTTGAAACTCTAGAATTAACTGCAATAAGTGAAGATGAAGTCCTTGATTTAGAATCCATTTCAACTCCACCACAATTTATTCGGAAACTGATCTTGTACGGGCGTTTAGAGCAGTTGCCAAGCTGGATTTCAAATCTTCAACATCTCGTCGCATTGGTGATTTATTGGTCAAGGTTGAGAGATTTCCCATTGAAAGCCCTTCAAAATCTACCTAATCTGTTGGATCTTGGTTTGGGACAAATGGGACATGAAGGCATGCGGCTGCATTTTGAGGAAGGAGGATTTCAGAAACTTAGGAAGCTCCGACTCAGGGAAGTGGAGGGACTAAATTCATTGATCATAGACAATGGGGTTATGCCTATTCTCCAAGAGTTTCACATTGGACCTAGCCCACAACTGAAGGATGTGCCATCCGGCATCTGCCGCCTTCGAAATCTGAGAGATCTTAGGTTTTATGACATGACGAAGGAGTTTGCACGACAAATGGATCCAAATAATGGCCCACATTATTGGATTGTTAAACATATACGACATGTTGATTTTATTTACAACTATGTCCCGAGATATGGCGAATATGAAACCCACTCCCTGTATGATTCCGACTTTTCATTTTGGTCGAATGTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0002376 GO:0003674 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0008219 GO:0009626 GO:0009987 GO:0012501 GO:0016020 GO:0019897 GO:0019898 GO:0023052 GO:0033554 GO:0034050 GO:0036094 GO:0044425 GO:0044459 GO:0044464 GO:0045087 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071944 GO:0097159 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko04626 map04626

Pfam Domains

Protein Families

NB-ARC

Protein Analysis

998

Amino Acids

115.55

Weight (kDa)

8.08

Isoelectric Point (pI)

41.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Rx_N	PF18052	57 - 148	7.1e-21	Rx N-terminal domain
NB-ARC	PF00931	235 - 406	1.3e-41	NB-ARC domain
WHD_DRP	PF23559	494 - 563	7.5e-23	Disease resistance protein Winged helix domain
LRR_14	PF23598	609 - 939	1.9e-46	Leucine-rich repeat region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003960)

Species	Orthologous Gene IDs
malus_domestica	MD00G1097800.v1.1 MD01G1186500.v1.1 MD01G1186600.v1.1 MD01G1186800.v1.1 MD01G1187000.v1.1 MD01G1187100.v1.1 MD07G1258600.v1.1 MD07G1258700.v1.1 MD07G1258800.v1.1 MD07G1258900.v1.1 MD07G1259200.v1.1 MD07G1259300.v1.1
prunus_persica	Prupe.2G283200_v2.0.a1 Prupe.2G283300_v2.0.a1 Prupe.2G283400_v2.0.a1
pyrus_communis	pycom01g19810 pycom01g19820 pycom01g19840 pycom01g19900 pycom07g23170 pycom07g23230 pycom07g23240 pycom07g23250 pycom07g23260
rosa_chinensis	RchiOBHm_Chr1g0375241
rosa_laevigata	RLG00000026676
rosa_multiflora	Rmu_co8330553.1_g000001 Rmu_sc0000554.1_g000032
rosa_roxburghii	Rroxscaffold_4G00282910
rosa_rugosa	Rorug01G0389400
rosa_samantha	Rh1AG400100 Rh1BG364400 Rh1DG395000
rosa_wichuraiana	Rw1G035520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1755
AatII	GACGTC	1 cut(s)	1774
AccB1I	GGYRCC	1 cut(s)	429
AccB7I	CCANNNNNTGG	1 cut(s)	2864
AccII	CGCG	2 cut(s)	738, 1686
AciI	CCGC	8 cut(s)	314, 455, 1077, 1115, 2116, 2293, 2626, 2791
AclWI	GGATC	6 cut(s)	882, 2064, 2261, 2598, 2849, 2862
AcoI	YGGCCR	1 cut(s)	1078
AcsI	RAATTY	3 cut(s)	1739, 1827, 2695
AcuI	CTGAAG	3 cut(s)	690, 1557, 2786
AcyI	GRCGYC	1 cut(s)	1771
AfaI	GTAC	4 cut(s)	1001, 1982, 2228, 2465
AfiI	CCNNNNNNNGG	6 cut(s)	591, 643, 1702, 2677, 2864, 2933
AflII	CTTAAG	2 cut(s)	1369, 1984
AflIII	ACRYGT	1 cut(s)	2899
AhdI	GACNNNNNGTC	1 cut(s)	955
AjnI	CCWGG	3 cut(s)	788, 1617, 2052
AjuI	GAANNNNNNNTTGG	2 cut(s)	2479, 2511
Alw21I	GWGCWC	1 cut(s)	1695
Alw26I	GTCTC	5 cut(s)	228, 323, 727, 1778, 1827
AlwI	GGATC	6 cut(s)	882, 2064, 2261, 2598, 2849, 2862
Ama87I	CYCGRG	4 cut(s)	639, 1804, 1865, 2926
AoxI	GGCC	6 cut(s)	1078, 1405, 1862, 2127, 2302, 2866
ApeKI	GCWGC	4 cut(s)	377, 988, 2113, 2629
ApoI	RAATTY	3 cut(s)	1739, 1827, 2695
ArsI	GACNNNNNNTTYG	4 cut(s)	342, 374, 1449, 1481
AseI	ATTAAT	1 cut(s)	1503
Asp700I	GAANNNNTTC	1 cut(s)	540
AspLEI	GCGC	2 cut(s)	730, 1686
AspS9I	GGNCC	4 cut(s)	820, 1863, 2750, 2867
AsuHPI	GGTGA	4 cut(s)	202, 826, 2357, 2536
AsuII	TTCGAA	2 cut(s)	515, 2797
AvaI	CYCGRG	4 cut(s)	639, 1804, 1865, 2926
AvaII	GGWCC	2 cut(s)	820, 2750
AxyI	CCTNAGG	1 cut(s)	1991
BaeI	ACNNNNGTAYC	2 cut(s)	1511, 1544
BamHI	GGATCC	1 cut(s)	2854
BanI	GGYRCC	1 cut(s)	429
BbsI	GAAGAC	2 cut(s)	568, 782
Bbv12I	GWGCWC	1 cut(s)	1695
BbvCI	CCTCAGC	1 cut(s)	1427
BbvI	GCAGC	4 cut(s)	389, 1000, 2100, 2616
BccI	CCATC	7 cut(s)	317, 497, 811, 1158, 1640, 2321, 2785
BciT130I	CCWGG	3 cut(s)	790, 1619, 2054
BclI	TGATCA	2 cut(s)	963, 2703
BcoDI	GTCTC	5 cut(s)	228, 323, 727, 1778, 1827
BfaI	CTAG	5 cut(s)	120, 272, 1238, 2378, 2754
BfmI	CTRYAG	2 cut(s)	1047, 2143
BfoI	RGCGCY	1 cut(s)	731
BfrI	CTTAAG	2 cut(s)	1369, 1984
BglII	AGATCT	1 cut(s)	2809
BisI	GCNGC	9 cut(s)	315, 378, 989, 1078, 2114, 2117, 2627, 2630, 2791
BlsI	GCNGC	9 cut(s)	316, 379, 990, 1079, 2115, 2118, 2628, 2631, 2792
Bme1390I	CCNGG	3 cut(s)	790, 1619, 2054
Bme18I	GGWCC	2 cut(s)	820, 2750
BmeRI	GACNNNNNGTC	1 cut(s)	955
BmeT110I	CYCGRG	4 cut(s)	639, 1804, 1865, 2926
BmgT120I	GGNCC	4 cut(s)	820, 1863, 2750, 2867
BmiI	GGNNCC	2 cut(s)	431, 2856
BmrFI	CCNGG	3 cut(s)	790, 1619, 2054
BmrI	ACTGGG	2 cut(s)	850, 2282
BmuI	ACTGGG	2 cut(s)	850, 2282
BoxI	GACNNNNGTC	1 cut(s)	890
BpiI	GAAGAC	2 cut(s)	568, 782
BplI	GAGNNNNNCTC	2 cut(s)	1481, 1513
Bpu10I	CCTNAGC	2 cut(s)	309, 1427
Bpu14I	TTCGAA	2 cut(s)	515, 2797
BpuEI	CTTGAG	1 cut(s)	278
BsaBI	GATNNNNATC	1 cut(s)	1968
BsaHI	GRCGYC	1 cut(s)	1771
BsaJI	CCNNGG	2 cut(s)	1803, 2052
BsaWI	WCCGGW	2 cut(s)	474, 1063
Bsc4I	CCNNNNNNNGG	6 cut(s)	591, 643, 1702, 2677, 2864, 2933
Bse118I	RCCGGY	1 cut(s)	591
Bse1I	ACTGG	4 cut(s)	856, 1210, 1575, 2277
Bse21I	CCTNAGG	1 cut(s)	1991
Bse8I	GATNNNNATC	1 cut(s)	1968
BseBI	CCWGG	3 cut(s)	790, 1619, 2054
BseDI	CCNNGG	2 cut(s)	1803, 2052
BseGI	GGATG	7 cut(s)	149, 279, 508, 1933, 2207, 2776, 2777
BseJI	GATNNNNATC	1 cut(s)	1968
BseLI	CCNNNNNNNGG	6 cut(s)	591, 643, 1702, 2677, 2864, 2933
BseMII	CTCAG	7 cut(s)	592, 872, 1441, 1590, 1982, 2689, 2795
BseNI	ACTGG	4 cut(s)	856, 1210, 1575, 2277
BseRI	GAGGAG	4 cut(s)	677, 738, 968, 2190
BseX3I	CGGCCG	1 cut(s)	1078
BseXI	GCAGC	4 cut(s)	389, 1000, 2100, 2616
Bsh1236I	CGCG	2 cut(s)	738, 1686
Bsh1285I	CGRYCG	1 cut(s)	1081
BshFI	GGCC	6 cut(s)	1080, 1407, 1864, 2129, 2304, 2868
BshNI	GGYRCC	1 cut(s)	429
BsiEI	CGRYCG	1 cut(s)	1081
BsiHKAI	GWGCWC	1 cut(s)	1695
BsiHKCI	CYCGRG	4 cut(s)	639, 1804, 1865, 2926
BsiSI	CCGG	4 cut(s)	475, 592, 1064, 2781
BslFI	GGGAC	7 cut(s)	838, 943, 2289, 2616, 2625, 2703, 2909
BslI	CCNNNNNNNGG	6 cut(s)	591, 643, 1702, 2677, 2864, 2933
BsmAI	GTCTC	5 cut(s)	228, 323, 727, 1778, 1827
BsmBI	CGTCTC	4 cut(s)	228, 323, 727, 1778
BsmFI	GGGAC	7 cut(s)	838, 943, 2289, 2616, 2625, 2703, 2909
BsmI	GAATGC	2 cut(s)	1183, 1205
BsnI	GGCC	6 cut(s)	1080, 1407, 1864, 2129, 2304, 2868
BsoBI	CYCGRG	4 cut(s)	639, 1804, 1865, 2926
Bsp119I	TTCGAA	2 cut(s)	515, 2797
Bsp1286I	GDGCHC	1 cut(s)	1695
BspACI	CCGC	8 cut(s)	314, 455, 1077, 1115, 2116, 2293, 2626, 2791
BspANI	GGCC	6 cut(s)	1080, 1407, 1864, 2129, 2304, 2868
BspCNI	CTCAG	7 cut(s)	591, 873, 1440, 1591, 1983, 2688, 2796
BspFNI	CGCG	2 cut(s)	738, 1686
BspHI	TCATGA	1 cut(s)	1672
BspLI	GGNNCC	2 cut(s)	431, 2856
BspPI	GGATC	6 cut(s)	882, 2064, 2261, 2598, 2849, 2862
BspT104I	TTCGAA	2 cut(s)	515, 2797
BspT107I	GGYRCC	1 cut(s)	429
BspTI	CTTAAG	2 cut(s)	1369, 1984
BsrFI	RCCGGY	1 cut(s)	591
BsrI	ACTGG	4 cut(s)	856, 1210, 1575, 2277
BssAI	RCCGGY	1 cut(s)	591
BssECI	CCNNGG	2 cut(s)	1803, 2052
BssNI	GRCGYC	1 cut(s)	1771
Bst2UI	CCWGG	3 cut(s)	790, 1619, 2054
Bst6I	CTCTTC	2 cut(s)	627, 1569
BstACI	GRCGYC	1 cut(s)	1771
BstAFI	CTTAAG	2 cut(s)	1369, 1984
BstAPI	GCANNNNNTGC	1 cut(s)	1165
BstBI	TTCGAA	2 cut(s)	515, 2797
BstC8I	GCNNGC	1 cut(s)	2624
BstENI	CCTNNNNNAGG	1 cut(s)	1700
BstF5I	GGATG	7 cut(s)	149, 279, 508, 1933, 2207, 2776, 2777
BstFNI	CGCG	2 cut(s)	738, 1686
BstH2I	RGCGCY	1 cut(s)	731
BstHHI	GCGC	2 cut(s)	730, 1686
BstMAI	GTCTC	5 cut(s)	228, 323, 727, 1778, 1827
BstMCI	CGRYCG	1 cut(s)	1081
BstMWI	GCNNNNNNNGC	7 cut(s)	314, 1165, 1690, 2110, 2342, 2476, 2790
BstNI	CCWGG	3 cut(s)	790, 1619, 2054
BstNSI	RCATGY	3 cut(s)	1058, 2626, 2903
BstPAI	GACNNNNGTC	1 cut(s)	890
BstSCI	CCNGG	3 cut(s)	788, 1617, 2052
BstSFI	CTRYAG	2 cut(s)	1047, 2143
BstUI	CGCG	2 cut(s)	738, 1686
BstV1I	GCAGC	4 cut(s)	389, 1000, 2100, 2616
BstV2I	GAAGAC	2 cut(s)	568, 782
BstX2I	RGATCY	5 cut(s)	874, 2056, 2590, 2809, 2854
BstXI	CCANNNNNNTGG	1 cut(s)	2877
BstYI	RGATCY	5 cut(s)	874, 2056, 2590, 2809, 2854
BstZI	CGGCCG	1 cut(s)	1078
Bsu36I	CCTNAGG	1 cut(s)	1991
BsuRI	GGCC	6 cut(s)	1080, 1407, 1864, 2129, 2304, 2868
BtsCI	GGATG	7 cut(s)	149, 279, 508, 1933, 2207, 2776, 2777
BtsIMutI	CAGTG	3 cut(s)	134, 349, 1568
Cac8I	GCNNGC	1 cut(s)	2624
CciI	TCATGA	1 cut(s)	1672
CfoI	GCGC	2 cut(s)	730, 1686
Cfr10I	RCCGGY	1 cut(s)	591
Cfr13I	GGNCC	4 cut(s)	820, 1863, 2750, 2867
CseI	GACGC	2 cut(s)	308, 744
Csp6I	GTAC	4 cut(s)	1000, 1981, 2227, 2464
CspCI	CAANNNNNGTGG	2 cut(s)	1938, 1973
CviQI	GTAC	4 cut(s)	1000, 1981, 2227, 2464
DraI	TTTAAA	2 cut(s)	829, 1900
DriI	GACNNNNNGTC	1 cut(s)	955
EaeI	YGGCCR	1 cut(s)	1078
EagI	CGGCCG	1 cut(s)	1078
Eam1104I	CTCTTC	2 cut(s)	627, 1569
Eam1105I	GACNNNNNGTC	1 cut(s)	955
EarI	CTCTTC	2 cut(s)	627, 1569
EclXI	CGGCCG	1 cut(s)	1078
Eco147I	AGGCCT	2 cut(s)	1407, 2304
Eco32I	GATATC	2 cut(s)	37, 747
Eco47I	GGWCC	2 cut(s)	820, 2750
Eco52I	CGGCCG	1 cut(s)	1078
Eco57I	CTGAAG	3 cut(s)	690, 1557, 2786
Eco81I	CCTNAGG	1 cut(s)	1991
Eco88I	CYCGRG	4 cut(s)	639, 1804, 1865, 2926
EcoNI	CCTNNNNNAGG	1 cut(s)	1700
EcoRII	CCWGG	3 cut(s)	788, 1617, 2052
EcoRV	GATATC	2 cut(s)	37, 747
EcoT22I	ATGCAT	2 cut(s)	1060, 1183
Esp3I	CGTCTC	4 cut(s)	228, 323, 727, 1778
FalI	AAGNNNNNCTT	6 cut(s)	210, 242, 908, 940, 1680, 1712
FaqI	GGGAC	7 cut(s)	838, 943, 2289, 2616, 2625, 2703, 2909
FauNDI	CATATG	1 cut(s)	13
FbaI	TGATCA	2 cut(s)	963, 2703
Fnu4HI	GCNGC	9 cut(s)	315, 378, 989, 1078, 2114, 2117, 2627, 2630, 2791
FokI	GGATG	7 cut(s)	156, 266, 515, 1940, 2214, 2764, 2783
Fsp4HI	GCNGC	9 cut(s)	315, 378, 989, 1078, 2114, 2117, 2627, 2630, 2791
FspBI	CTAG	5 cut(s)	120, 272, 1238, 2378, 2754
GlaI	GCGC	2 cut(s)	729, 1685
GluI	GCNGC	9 cut(s)	315, 378, 989, 1078, 2114, 2117, 2627, 2630, 2791
HaeII	RGCGCY	1 cut(s)	731
HaeIII	GGCC	6 cut(s)	1080, 1407, 1864, 2129, 2304, 2868
HapII	CCGG	4 cut(s)	475, 592, 1064, 2781
HgaI	GACGC	2 cut(s)	308, 744
HhaI	GCGC	2 cut(s)	730, 1686
Hin1I	GRCGYC	1 cut(s)	1771
Hin6I	GCGC	2 cut(s)	728, 1684
HinP1I	GCGC	2 cut(s)	728, 1684
HincII	GTYRAC	4 cut(s)	361, 523, 1323, 1749
HindII	GTYRAC	4 cut(s)	361, 523, 1323, 1749
HindIII	AAGCTT	3 cut(s)	1717, 2102, 2211
HpaII	CCGG	4 cut(s)	475, 592, 1064, 2781
HphI	GGTGA	4 cut(s)	202, 826, 2357, 2536
Hpy166II	GTNNAC	5 cut(s)	361, 523, 1323, 1639, 1749
Hpy8I	GTNNAC	5 cut(s)	361, 523, 1323, 1639, 1749
Hpy99I	CGWCG	2 cut(s)	1773, 2519
HpyCH4IV	ACGT	5 cut(s)	237, 1002, 1771, 1979, 2320
HpyF10VI	GCNNNNNNNGC	7 cut(s)	314, 1165, 1690, 2110, 2342, 2476, 2790
HpySE526I	ACGT	5 cut(s)	237, 1002, 1771, 1979, 2320
Hsp92I	GRCGYC	1 cut(s)	1771
HspAI	GCGC	2 cut(s)	728, 1684
Ksp22I	TGATCA	2 cut(s)	963, 2703
LmnI	GCTCC	4 cut(s)	725, 1698, 1936, 2673
Lsp1109I	GCAGC	4 cut(s)	389, 1000, 2100, 2616
MaeI	CTAG	5 cut(s)	120, 272, 1238, 2378, 2754
MaeII	ACGT	5 cut(s)	237, 1002, 1771, 1979, 2320
MaeIII	GTNAC	3 cut(s)	421, 1254, 2138
MfeI	CAATTG	1 cut(s)	1296
MflI	RGATCY	5 cut(s)	874, 2056, 2590, 2809, 2854
MhlI	GDGCHC	1 cut(s)	1695
MlyI	GAGTC	5 cut(s)	613, 965, 1877, 2015, 2667
MmeI	TCCRAC	6 cut(s)	121, 700, 1561, 2568, 2695, 2991
Mph1103I	ATGCAT	2 cut(s)	1060, 1183
MroXI	GAANNNNTTC	1 cut(s)	540
MspCI	CTTAAG	2 cut(s)	1369, 1984
MspI	CCGG	4 cut(s)	475, 592, 1064, 2781
MspR9I	CCNGG	3 cut(s)	790, 1619, 2054
MunI	CAATTG	1 cut(s)	1296
Mva1269I	GAATGC	2 cut(s)	1183, 1205
MvaI	CCWGG	3 cut(s)	790, 1619, 2054
MvnI	CGCG	2 cut(s)	738, 1686
MwoI	GCNNNNNNNGC	7 cut(s)	314, 1165, 1690, 2110, 2342, 2476, 2790
NdeI	CATATG	1 cut(s)	13
NlaIV	GGNNCC	2 cut(s)	431, 2856
NmeAIII	GCCGAG	1 cut(s)	756
NmuCI	GTSAC	3 cut(s)	421, 1254, 2138
NsiI	ATGCAT	2 cut(s)	1060, 1183
NspI	RCATGY	3 cut(s)	1058, 2626, 2903
NspV	TTCGAA	2 cut(s)	515, 2797
PacI	TTAATTAA	1 cut(s)	198
PaeI	GCATGC	1 cut(s)	2626
PaeR7I	CTCGAG	1 cut(s)	639
PagI	TCATGA	1 cut(s)	1672
PceI	AGGCCT	2 cut(s)	1407, 2304
PciI	ACATGT	1 cut(s)	2899
PctI	GAATGC	2 cut(s)	1183, 1205
PdmI	GAANNNNTTC	1 cut(s)	540
PfeI	GAWTC	9 cut(s)	62, 686, 1102, 1416, 1712, 1809, 2242, 2420, 2963
PflFI	GACNNNGTC	1 cut(s)	344
PflMI	CCANNNNNTGG	1 cut(s)	2864
PkrI	GCNGC	9 cut(s)	316, 379, 990, 1079, 2115, 2118, 2628, 2631, 2792
PleI	GAGTC	5 cut(s)	613, 964, 1876, 2015, 2667
PpsI	GAGTC	5 cut(s)	613, 964, 1876, 2015, 2667
PscI	ACATGT	1 cut(s)	2899
PshAI	GACNNNNGTC	1 cut(s)	890
PshBI	ATTAAT	1 cut(s)	1503
PsiI	TTATAA	1 cut(s)	1755
Psp6I	CCWGG	3 cut(s)	788, 1617, 2052
PspGI	CCWGG	3 cut(s)	788, 1617, 2052
PspN4I	GGNNCC	2 cut(s)	431, 2856
PspPI	GGNCC	4 cut(s)	820, 1863, 2750, 2867
PspXI	VCTCGAGB	1 cut(s)	639
PsrI	GAACNNNNNNTAC	2 cut(s)	1044, 1076
PsuI	RGATCY	5 cut(s)	874, 2056, 2590, 2809, 2854
PsyI	GACNNNGTC	1 cut(s)	344
RsaI	GTAC	4 cut(s)	1001, 1982, 2228, 2465
RsaNI	GTAC	4 cut(s)	1000, 1981, 2227, 2464
SatI	GCNGC	9 cut(s)	315, 378, 989, 1078, 2114, 2117, 2627, 2630, 2791
Sau96I	GGNCC	4 cut(s)	820, 1863, 2750, 2867
SchI	GAGTC	5 cut(s)	613, 965, 1877, 2015, 2667
ScrFI	CCNGG	3 cut(s)	790, 1619, 2054
SduI	GDGCHC	1 cut(s)	1695
SfcI	CTRYAG	2 cut(s)	1047, 2143
Sfr274I	CTCGAG	1 cut(s)	639
SfuI	TTCGAA	2 cut(s)	515, 2797
SinI	GGWCC	2 cut(s)	820, 2750
SlaI	CTCGAG	1 cut(s)	639
SmlI	CTYRAG	4 cut(s)	293, 639, 1369, 1984
SmoI	CTYRAG	4 cut(s)	293, 639, 1369, 1984
SphI	GCATGC	1 cut(s)	2626
SseBI	AGGCCT	2 cut(s)	1407, 2304
SsiI	CCGC	8 cut(s)	314, 455, 1077, 1115, 2116, 2293, 2626, 2791
SspI	AATATT	2 cut(s)	410, 1592
SspMI	CTAG	5 cut(s)	120, 272, 1238, 2378, 2754
StuI	AGGCCT	2 cut(s)	1407, 2304
StyD4I	CCNGG	3 cut(s)	788, 1617, 2052
TaiI	ACGT	5 cut(s)	240, 1005, 1774, 1982, 2323
TatI	WGTACW	1 cut(s)	2226
TauI	GCSGC	5 cut(s)	317, 1080, 2119, 2629, 2793
TfiI	GAWTC	9 cut(s)	62, 686, 1102, 1416, 1712, 1809, 2242, 2420, 2963
TscAI	CASTG	3 cut(s)	134, 349, 1575
TseFI	GTSAC	3 cut(s)	421, 1254, 2138
TseI	GCWGC	4 cut(s)	377, 988, 2113, 2629
Tsp45I	GTSAC	3 cut(s)	421, 1254, 2138
TspGWI	ACGGA	1 cut(s)	962
TspRI	CASTG	3 cut(s)	134, 349, 1575
Tth111I	GACNNNGTC	1 cut(s)	344
Van91I	CCANNNNNTGG	1 cut(s)	2864
Vha464I	CTTAAG	2 cut(s)	1369, 1984
VpaK11BI	GGWCC	2 cut(s)	820, 2750
VspI	ATTAAT	1 cut(s)	1503
XagI	CCTNNNNNAGG	1 cut(s)	1700
XapI	RAATTY	3 cut(s)	1739, 1827, 2695
XbaI	TCTAGA	2 cut(s)	1237, 2377
XceI	RCATGY	3 cut(s)	1058, 2626, 2903
XhoI	CTCGAG	1 cut(s)	639
XmnI	GAANNNNTTC	1 cut(s)	540
XspI	CTAG	5 cut(s)	120, 272, 1238, 2378, 2754
ZraI	GACGTC	1 cut(s)	1772
Zsp2I	ATGCAT	2 cut(s)	1060, 1183

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.