Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0003960

Physical Location & Seq

Genomic Coordinates Forward (+)

28517385 .. 28520196

Gene Structure 2812 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1186500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2673 bp

ATGGCAGAATCAGTTGTATGCTTTGTGATCGATAAGTTAATTTCACTACTCATCACCACAGAAGCGAAACTTTCAAGAGACGTGCGTGCTGAAGTTGGTTTTCTCAGAGATGAGCTTGAAAGCATCCGATCATTCCTCAAGGATGCAGACGCAGCAGCAGCAGCAGCAGCAGAAGGAGACATGGTGACTGACAGTGCCAAAACTTGGGTCAAACAAGTAAGGGAAGCAGCTTTTTACATTGAAGATGTCATCGATGAGTATTTGCTTCGTGTCACAAGGCACCATCAAGACCGCGGATTCGTTCGCTTTGTCCACAAAGTTGTTTGGTTTCTGAAGAAAATGAAACCACAGGATGAGATAGCCTCAAAAATAGAGGGCATGAGAGCATTGGTTAGTGAAATCAAGGCTAGACATGAAAGATATGGACTTGGTTCAATTGATCAGCAAGGTCAAAGCAGTGAAGAAATTGTTGTTTCGTGGCATGACCCTCGAGTGGCTTCCCTTTTCATCGAGGAAGCTGAAGTTGTTGGTGTGGAATCTGCCAGAGATGAGCTGATTAGTTGGTTGGTAGGAAAAGCATCAAGAGGCGAATACCGTGAAGTAATATCAGTGGTTGGCATGGGAGGACTTGGTAAGACCACTCTCGCCAAGAAAGTCTATGACAACCAAAAGGTGATCGAACACTTCGATTGCCATGCTTGGATTACAGTGTCTCAGACGTACAAGGTGGAGGATCTTCTGAGGCAGATGGCGATAGAACATGCTTTACCGGATAATAAAGGCGGAAGAATAGTGATCACCACGAGGATTAAAGATGTTGCTGATTTTTGCAAAAAGTCTTCTTATGTTCATGTTTACCGTTTGCAACCTCTGCCTCCAAACAAGGCATGGGAATTGTTTTGTAGAAGGGCGTTTCAATATGAATTGGAGGTGAACTGCCCTGCAGATTTGAAAGATTTGTCTCTTGAATTCGTTAGAAAGTGCGAAGGATTGCCTTTGGCAATTGTTTCCATAGGTGGTCTGCTGTCAACAAAAGTTAAAGTTGTGTCTGAATGGCAGAAGTTATACGATAGCTTAAGTTCAGAGTTAGAAAGCAATCCCCACCTTACAAGCCTCACAAGAATTCTATCCTTCAGCTATCATCATCTACCGTATCACCTCAAATCATGTGTCTTATACTTTGGCATCTTTCCCGAGGACCACTCAATTAGTTGCGTGAGACTAGCTCGGTTGTGGATCGCTGAAGGCTTTGTGAAACCGAAGAGAGGCAAGACACTAGAAGAGGTTGCAGAGGAATACTTGACTGAACTGATACATAGAAGCCTAGTGCAAGTGTCACAGGTTTGGATCGATGGGAAAGCTAGAAGCTGTCGAGTCCATGATCTGATGCGCGAAGTGCTCCTTAGAAAAGCGGTGGATTCAAATTTCTGTCATGTGTTATCAGAAAATGAGTCAACATTAAAGCCAATAACTCGACGCCTCTTGATAGACAGCACGCCCCATGGTGCCTTGGGAATAATTGAACAATCTCGCATTCGTTCTTTATTTACTTTCAACCAAGAACAATGGCCCGAGTCTTTTCTCAGTACACTGAGTGGAAACTTTAAACTTATGAAAATATTGGATTTTGCAGATGCTCCCCTGAATCATCTTCCGAAGTATGTGGGGGATTTGTATCTCTTGAAGTACTTAAGCCTAAGGAATACAAAGGTGAAGTTGCTTCCAGAGTCCATATGTAATCTGCAAAACTTAGAAACCCTGGATTTAAAACAGTCTCTGGTATATGAAATACCAGCCAAGATCAATAAGCTTCTAAGGTTGCGACATCTTTTGGCCCATTATACTGACTCCAGCATAGATTTCAGCATGATCAGTTACGAACGAGGAGTGAAAATTCACGATGATATTGGATGCTTACAAGCTTTGCAAGAGTTATACCATGTGGAAACAAATCACGGCGGAATCAACCTAATTAAAGCATTGGAAAAGTTGAGGCAACTGCGCAAGCTGGGCCTGAAAAACTTGAAACATGAATATGGACGAGCTCTATGTGCTTCCGTTGAAAAGATGAACCACCTTGAATCTCTAGAAGTAAGCACAATAAATGAAGATGAAGTCCTCGACCTACAATCCATTTCAACTCCACCCAAATCTATTCGGTTTCTCTACTTAAAGGGGCCTCTGGAGAAGTTGCCAAGTTGGATTCCAAAGCTTCAACAACTCGTCAAGTTACGAATTTTTTGGTCAAGGTTGACAAATGCCCCACTGAAAGCCCTTCAAAACCTTCCTAATTTGGTAGAGCTTGGGTTTTCATACAATGCATATGATGGTGTGCAACTGCATTTTGAGGGAGGCTTTAAGGAACTCAGGGTGTTGAAACTCAAGCACTTGCCCAGACTAAACTCATTGATCATAGACAATGGGGCTATGCCTCTTCTCCGAGAGCTACAAATTGGGCCTTCCCCACAACTGAAGGAGGTGCCCTCTGGCATCCAGCACCTCAGAAATCTAAGCTTTCTTAGATTTGTTGACATGCCAAAAGAGTTCCGACGACATATGGATCCAAATAATGGCCAACATTATTGGATTGTTGAACATGTTCTTTTTAGTTACAAGTTGGGACCAAGATGCGATGTATATGAAACTCACGCCCTGCGTGATTCCAACCTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0002376 GO:0003674 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0008219 GO:0009626 GO:0009987 GO:0012501 GO:0016020 GO:0019897 GO:0019898 GO:0023052 GO:0033554 GO:0034050 GO:0036094 GO:0044425 GO:0044459 GO:0044464 GO:0045087 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071944 GO:0097159 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko04626 map04626

Pfam Domains

Protein Families

NB-ARC

Protein Analysis

891

Amino Acids

101.96

Weight (kDa)

8.31

Isoelectric Point (pI)

41.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Rx_N	PF18052	5 - 105	1.7e-21	Rx N-terminal domain
NB-ARC	PF00931	183 - 251	1.2e-16	NB-ARC domain
WHD_DRP	PF23559	396 - 465	2.3e-23	Disease resistance protein Winged helix domain
LRR_14	PF23598	512 - 843	6.4e-44	Leucine-rich repeat region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0003960)

Species	Orthologous Gene IDs
malus_domestica	MD00G1097800.v1.1 MD01G1186500.v1.1 MD01G1186600.v1.1 MD01G1186800.v1.1 MD01G1187000.v1.1 MD01G1187100.v1.1 MD07G1258600.v1.1 MD07G1258700.v1.1 MD07G1258800.v1.1 MD07G1258900.v1.1 MD07G1259200.v1.1 MD07G1259300.v1.1
prunus_persica	Prupe.2G283200_v2.0.a1 Prupe.2G283300_v2.0.a1 Prupe.2G283400_v2.0.a1
pyrus_communis	pycom01g19810 pycom01g19820 pycom01g19840 pycom01g19900 pycom07g23170 pycom07g23230 pycom07g23240 pycom07g23250 pycom07g23260
rosa_chinensis	RchiOBHm_Chr1g0375241
rosa_laevigata	RLG00000026676
rosa_multiflora	Rmu_co8330553.1_g000001 Rmu_sc0000554.1_g000032
rosa_roxburghii	Rroxscaffold_4G00282910
rosa_rugosa	Rorug01G0389400
rosa_samantha	Rh1AG400100 Rh1BG364400 Rh1DG395000
rosa_wichuraiana	Rw1G035520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	2003
AccB1I	GGYRCC	3 cut(s)	279, 1505, 2479
AccB7I	CCANNNNNTGG	2 cut(s)	204, 2570
AccII	CGCG	2 cut(s)	294, 1392
AciI	CCGC	5 cut(s)	292, 294, 783, 1412, 1959
AclWI	GGATC	5 cut(s)	741, 1244, 1355, 2555, 2568
AcoI	YGGCCR	1 cut(s)	2572
AcsI	RAATTY	5 cut(s)	968, 1122, 1423, 1893, 2235
AcuI	CTGAAG	6 cut(s)	111, 353, 540, 1117, 1263, 2492
AcyI	GRCGYC	1 cut(s)	1477
AdeI	CACNNNGTG	1 cut(s)	1595
AfaI	GTAC	3 cut(s)	722, 1588, 1688
AfiI	CCNNNNNNNGG	4 cut(s)	204, 493, 1265, 2570
AflII	CTTAAG	2 cut(s)	1075, 1690
AflIII	ACRYGT	1 cut(s)	2596
AjiI	CACGTC	1 cut(s)	82
AjnI	CCWGG	1 cut(s)	1758
Alw21I	GWGCWC	2 cut(s)	1401, 2047
Alw26I	GTCTC	6 cut(s)	72, 171, 717, 966, 1213, 1779
AlwI	GGATC	5 cut(s)	741, 1244, 1355, 2555, 2568
AlwNI	CAGNNNCTG	1 cut(s)	1777
Ama87I	CYCGRG	3 cut(s)	489, 1193, 1571
AoxI	GGCC	6 cut(s)	1568, 1833, 2011, 2177, 2456, 2572
ApeKI	GCWGC	7 cut(s)	152, 155, 158, 161, 164, 167, 227
ApoI	RAATTY	5 cut(s)	968, 1122, 1423, 1893, 2235
ArsI	GACNNNNNNTTYG	6 cut(s)	192, 224, 281, 313, 1155, 1187
Asp700I	GAANNNNTTC	1 cut(s)	2598
AspLEI	GCGC	2 cut(s)	1392, 2004
AspS9I	GGNCC	7 cut(s)	1198, 1569, 1834, 2011, 2177, 2456, 2621
AsuHPI	GGTGA	7 cut(s)	46, 196, 685, 790, 943, 1148, 1723
AvaI	CYCGRG	3 cut(s)	489, 1193, 1571
AvaII	GGWCC	2 cut(s)	1198, 2621
AxyI	CCTNAGG	1 cut(s)	1697
BaeGI	GKGCMC	1 cut(s)	2484
BalI	TGGCCA	1 cut(s)	2574
BamHI	GGATCC	1 cut(s)	2560
BanI	GGYRCC	3 cut(s)	279, 1505, 2479
BanII	GRGCYC	1 cut(s)	2047
BauI	CACGAG	1 cut(s)	802
BbsI	GAAGAC	1 cut(s)	831
Bbv12I	GWGCWC	2 cut(s)	1401, 2047
BbvI	GCAGC	7 cut(s)	164, 167, 170, 173, 176, 179, 239
BccI	CCATC	4 cut(s)	291, 742, 1346, 2321
BceAI	ACGGC	1 cut(s)	1972
BcgI	CGANNNNNNTGC	4 cut(s)	470, 504, 677, 711
BciT130I	CCWGG	1 cut(s)	1760
BclI	TGATCA	4 cut(s)	439, 795, 1869, 2409
BcoDI	GTCTC	6 cut(s)	72, 171, 717, 966, 1213, 1779
BfaI	CTAG	6 cut(s)	408, 1223, 1277, 1325, 1362, 2087
BfmI	CTRYAG	1 cut(s)	942
BfrI	CTTAAG	2 cut(s)	1075, 1690
BisI	GCNGC	7 cut(s)	153, 156, 159, 162, 165, 168, 228
BlsI	GCNGC	7 cut(s)	154, 157, 160, 163, 166, 169, 229
BmcAI	AGTACT	1 cut(s)	1688
Bme1390I	CCNGG	1 cut(s)	1760
Bme18I	GGWCC	2 cut(s)	1198, 2621
BmeT110I	CYCGRG	3 cut(s)	489, 1193, 1571
BmgBI	CACGTC	1 cut(s)	82
BmgT120I	GGNCC	7 cut(s)	1198, 1569, 1834, 2011, 2177, 2456, 2621
BmiI	GGNNCC	6 cut(s)	281, 1507, 2178, 2481, 2562, 2622
BmrFI	CCNGG	1 cut(s)	1760
BmsI	GCATC	9 cut(s)	132, 133, 587, 1194, 1377, 1624, 1901, 2499, 2618
BpiI	GAAGAC	1 cut(s)	831
BplI	GAGNNNNNCTC	8 cut(s)	347, 379, 1187, 1219, 1210, 1242, 2468, 2500
BpmI	CTGGAG	2 cut(s)	1834, 2204
BpuEI	CTTGAG	2 cut(s)	122, 2366
Bsa29I	ATCGAT	3 cut(s)	30, 252, 1350
BsaBI	GATNNNNATC	1 cut(s)	1674
BsaHI	GRCGYC	1 cut(s)	1477
BsaJI	CCNNGG	5 cut(s)	292, 1194, 1501, 1509, 1758
BsaWI	WCCGGW	1 cut(s)	769
BsaXI	ACNNNNNCTCC	2 cut(s)	168, 198
Bsc4I	CCNNNNNNNGG	4 cut(s)	204, 493, 1265, 2570
Bse21I	CCTNAGG	1 cut(s)	1697
Bse8I	GATNNNNATC	1 cut(s)	1674
BseBI	CCWGG	1 cut(s)	1760
BseCI	ATCGAT	3 cut(s)	30, 252, 1350
BseDI	CCNNGG	5 cut(s)	292, 1194, 1501, 1509, 1758
BseGI	GGATG	5 cut(s)	123, 148, 358, 1916, 2490
BseJI	GATNNNNATC	1 cut(s)	1674
BseLI	CCNNNNNNNGG	4 cut(s)	204, 493, 1265, 2570
BseMII	CTCAG	7 cut(s)	118, 728, 731, 1583, 1597, 2380, 2515
BseRI	GAGGAG	1 cut(s)	1899
BseSI	GKGCMC	1 cut(s)	2484
BseXI	GCAGC	7 cut(s)	164, 167, 170, 173, 176, 179, 239
BseYI	CCCAGC	1 cut(s)	2008
Bsh1236I	CGCG	2 cut(s)	294, 1392
BshFI	GGCC	6 cut(s)	1570, 1835, 2013, 2179, 2458, 2574
BshNI	GGYRCC	3 cut(s)	279, 1505, 2479
BshVI	ATCGAT	3 cut(s)	30, 252, 1350
BsiHKAI	GWGCWC	2 cut(s)	1401, 2047
BsiHKCI	CYCGRG	3 cut(s)	489, 1193, 1571
BsiSI	CCGG	1 cut(s)	770
BslFI	GGGAC	1 cut(s)	2634
BslI	CCNNNNNNNGG	4 cut(s)	204, 493, 1265, 2570
BsmAI	GTCTC	6 cut(s)	72, 171, 717, 966, 1213, 1779
BsmBI	CGTCTC	1 cut(s)	72
BsmFI	GGGAC	1 cut(s)	2634
BsmI	GAATGC	1 cut(s)	1533
BsnI	GGCC	6 cut(s)	1570, 1835, 2013, 2179, 2458, 2574
BsoBI	CYCGRG	3 cut(s)	489, 1193, 1571
Bsp1286I	GDGCHC	3 cut(s)	1401, 2047, 2484
Bsp19I	CCATGG	1 cut(s)	1501
BspACI	CCGC	5 cut(s)	292, 294, 783, 1412, 1959
BspANI	GGCC	6 cut(s)	1570, 1835, 2013, 2179, 2458, 2574
BspCNI	CTCAG	7 cut(s)	117, 727, 732, 1584, 1596, 2379, 2514
BspDI	ATCGAT	3 cut(s)	30, 252, 1350
BspFNI	CGCG	2 cut(s)	294, 1392
BspLI	GGNNCC	6 cut(s)	281, 1507, 2178, 2481, 2562, 2622
BspMAI	CTGCAG	1 cut(s)	946
BspPI	GGATC	5 cut(s)	741, 1244, 1355, 2555, 2568
BspT107I	GGYRCC	3 cut(s)	279, 1505, 2479
BspTI	CTTAAG	2 cut(s)	1075, 1690
BssECI	CCNNGG	5 cut(s)	292, 1194, 1501, 1509, 1758
BssNI	GRCGYC	1 cut(s)	1477
BssSI	CACGAG	1 cut(s)	802
BssT1I	CCWWGG	2 cut(s)	1501, 1509
Bst2BI	CACGAG	1 cut(s)	802
Bst2UI	CCWGG	1 cut(s)	1760
Bst4CI	ACNGT	6 cut(s)	194, 596, 709, 860, 1152, 1773
Bst6I	CTCTTC	3 cut(s)	1256, 1275, 2439
BstACI	GRCGYC	1 cut(s)	1477
BstAFI	CTTAAG	2 cut(s)	1075, 1690
BstAPI	GCANNNNNTGC	1 cut(s)	871
BstC8I	GCNNGC	3 cut(s)	87, 1496, 2006
BstDSI	CCRYGG	2 cut(s)	292, 1501
BstF5I	GGATG	5 cut(s)	123, 148, 358, 1916, 2490
BstFNI	CGCG	2 cut(s)	294, 1392
BstHHI	GCGC	2 cut(s)	1392, 2004
BstMAI	GTCTC	6 cut(s)	72, 171, 717, 966, 1213, 1779
BstMWI	GCNNNNNNNGC	9 cut(s)	152, 158, 161, 164, 167, 871, 1396, 2010, 2051
BstNI	CCWGG	1 cut(s)	1760
BstNSI	RCATGY	3 cut(s)	764, 2536, 2600
BstSCI	CCNGG	1 cut(s)	1758
BstSFI	CTRYAG	1 cut(s)	942
BstSLI	GKGCMC	1 cut(s)	2484
BstUI	CGCG	2 cut(s)	294, 1392
BstV1I	GCAGC	7 cut(s)	164, 167, 170, 173, 176, 179, 239
BstV2I	GAAGAC	1 cut(s)	831
BstX2I	RGATCY	2 cut(s)	733, 2560
BstYI	RGATCY	2 cut(s)	733, 2560
Bsu15I	ATCGAT	3 cut(s)	30, 252, 1350
Bsu36I	CCTNAGG	1 cut(s)	1697
BsuRI	GGCC	6 cut(s)	1570, 1835, 2013, 2179, 2458, 2574
BsuTUI	ATCGAT	3 cut(s)	30, 252, 1350
BtgI	CCRYGG	2 cut(s)	292, 1501
BtgZI	GCGATG	1 cut(s)	2646
BtrI	CACGTC	1 cut(s)	82
BtsCI	GGATG	5 cut(s)	123, 148, 358, 1916, 2490
BtsI	GCAGTG	1 cut(s)	463
BtsIMutI	CAGTG	6 cut(s)	199, 463, 615, 714, 1589, 2264
Cac8I	GCNNGC	3 cut(s)	87, 1496, 2006
CaiI	CAGNNNCTG	1 cut(s)	1777
CfoI	GCGC	2 cut(s)	1392, 2004
Cfr13I	GGNCC	7 cut(s)	1198, 1569, 1834, 2011, 2177, 2456, 2621
Cfr42I	CCGCGG	1 cut(s)	295
ClaI	ATCGAT	3 cut(s)	30, 252, 1350
CseI	GACGC	2 cut(s)	158, 1485
Csp6I	GTAC	3 cut(s)	721, 1587, 1687
CviQI	GTAC	3 cut(s)	721, 1587, 1687
DraI	TTTAAA	2 cut(s)	1606, 1767
DraIII	CACNNNGTG	1 cut(s)	1595
EaeI	YGGCCR	1 cut(s)	2572
Eam1104I	CTCTTC	3 cut(s)	1256, 1275, 2439
EarI	CTCTTC	3 cut(s)	1256, 1275, 2439
EciI	GGCGGA	2 cut(s)	798, 1974
Ecl136II	GAGCTC	1 cut(s)	2045
Eco130I	CCWWGG	2 cut(s)	1501, 1509
Eco24I	GRGCYC	1 cut(s)	2047
Eco47I	GGWCC	2 cut(s)	1198, 2621
Eco53kI	GAGCTC	1 cut(s)	2045
Eco57I	CTGAAG	6 cut(s)	111, 353, 540, 1117, 1263, 2492
Eco81I	CCTNAGG	1 cut(s)	1697
Eco88I	CYCGRG	3 cut(s)	489, 1193, 1571
EcoICRI	GAGCTC	1 cut(s)	2045
EcoO109I	RGGNCCY	1 cut(s)	2177
EcoRI	GAATTC	2 cut(s)	968, 1122
EcoRII	CCWGG	1 cut(s)	1758
EcoT14I	CCWWGG	2 cut(s)	1501, 1509
EcoT22I	ATGCAT	1 cut(s)	2323
EcoT38I	GRGCYC	1 cut(s)	2047
ErhI	CCWWGG	2 cut(s)	1501, 1509
Esp3I	CGTCTC	1 cut(s)	72
FalI	AAGNNNNNCTT	4 cut(s)	54, 86, 1386, 1418
FaqI	GGGAC	1 cut(s)	2634
FauNDI	CATATG	3 cut(s)	1733, 2323, 2556
FbaI	TGATCA	4 cut(s)	439, 795, 1869, 2409
Fnu4HI	GCNGC	7 cut(s)	153, 156, 159, 162, 165, 168, 228
FokI	GGATG	5 cut(s)	110, 155, 365, 1923, 2477
FriOI	GRGCYC	1 cut(s)	2047
Fsp4HI	GCNGC	7 cut(s)	153, 156, 159, 162, 165, 168, 228
FspBI	CTAG	6 cut(s)	408, 1223, 1277, 1325, 1362, 2087
FspI	TGCGCA	1 cut(s)	2003
GlaI	GCGC	2 cut(s)	1391, 2003
GluI	GCNGC	7 cut(s)	153, 156, 159, 162, 165, 168, 228
GsaI	CCCAGC	1 cut(s)	2012
GsuI	CTGGAG	2 cut(s)	1834, 2204
HaeIII	GGCC	6 cut(s)	1570, 1835, 2013, 2179, 2458, 2574
HapII	CCGG	1 cut(s)	770
HgaI	GACGC	2 cut(s)	158, 1485
HhaI	GCGC	2 cut(s)	1392, 2004
Hin1I	GRCGYC	1 cut(s)	1477
Hin6I	GCGC	2 cut(s)	1390, 2002
HinP1I	GCGC	2 cut(s)	1390, 2002
HincII	GTYRAC	4 cut(s)	1029, 1455, 2253, 2530
HindII	GTYRAC	4 cut(s)	1029, 1455, 2253, 2530
HindIII	AAGCTT	4 cut(s)	1808, 1920, 2210, 2512
HpaII	CCGG	1 cut(s)	770
HphI	GGTGA	7 cut(s)	46, 196, 685, 790, 943, 1148, 1723
Hpy166II	GTNNAC	8 cut(s)	313, 856, 934, 1029, 1455, 1589, 2253, 2530
Hpy8I	GTNNAC	8 cut(s)	313, 856, 934, 1029, 1455, 1589, 2253, 2530
Hpy99I	CGWCG	2 cut(s)	1479, 2553
HpyAV	CCTTC	9 cut(s)	167, 900, 980, 1141, 1238, 2285, 2294, 2467, 2469
HpyCH4III	ACNGT	6 cut(s)	194, 596, 709, 860, 1152, 1773
HpyCH4IV	ACGT	2 cut(s)	81, 719
HpyF10VI	GCNNNNNNNGC	9 cut(s)	152, 158, 161, 164, 167, 871, 1396, 2010, 2051
HpySE526I	ACGT	2 cut(s)	81, 719
Hsp92I	GRCGYC	1 cut(s)	1477
HspAI	GCGC	2 cut(s)	1390, 2002
Ksp22I	TGATCA	4 cut(s)	439, 795, 1869, 2409
KspI	CCGCGG	1 cut(s)	295
LmnI	GCTCC	2 cut(s)	1404, 1642
Lsp1109I	GCAGC	7 cut(s)	164, 167, 170, 173, 176, 179, 239
LweI	GCATC	9 cut(s)	132, 133, 587, 1194, 1377, 1624, 1901, 2499, 2618
MaeI	CTAG	6 cut(s)	408, 1223, 1277, 1325, 1362, 2087
MaeII	ACGT	2 cut(s)	81, 719
MaeIII	GTNAC	6 cut(s)	184, 271, 1335, 1874, 2229, 2609
MfeI	CAATTG	2 cut(s)	435, 1002
MflI	RGATCY	2 cut(s)	733, 2560
MhlI	GDGCHC	3 cut(s)	1401, 2047, 2484
MlsI	TGGCCA	1 cut(s)	2574
MluNI	TGGCCA	1 cut(s)	2574
MlyI	GAGTC	5 cut(s)	1383, 1460, 1583, 1736, 1841
MmeI	TCCRAC	2 cut(s)	2180, 2572
Mox20I	TGGCCA	1 cut(s)	2574
Mph1103I	ATGCAT	1 cut(s)	2323
MroXI	GAANNNNTTC	1 cut(s)	2598
MscI	TGGCCA	1 cut(s)	2574
MslI	CAYNNNNRTG	1 cut(s)	2034
Msp20I	TGGCCA	1 cut(s)	2574
MspA1I	CMGCKG	1 cut(s)	294
MspCI	CTTAAG	2 cut(s)	1075, 1690
MspI	CCGG	1 cut(s)	770
MspR9I	CCNGG	1 cut(s)	1760
MunI	CAATTG	2 cut(s)	435, 1002
Mva1269I	GAATGC	1 cut(s)	1533
MvaI	CCWGG	1 cut(s)	1760
MvnI	CGCG	2 cut(s)	294, 1392
MwoI	GCNNNNNNNGC	9 cut(s)	152, 158, 161, 164, 167, 871, 1396, 2010, 2051
NcoI	CCATGG	1 cut(s)	1501
NdeI	CATATG	3 cut(s)	1733, 2323, 2556
NlaIV	GGNNCC	6 cut(s)	281, 1507, 2178, 2481, 2562, 2622
NmuCI	GTSAC	3 cut(s)	184, 271, 1335
NsbI	TGCGCA	1 cut(s)	2003
NsiI	ATGCAT	1 cut(s)	2323
NspI	RCATGY	3 cut(s)	764, 2536, 2600
PaeR7I	CTCGAG	1 cut(s)	489
PciI	ACATGT	1 cut(s)	2596
PcsI	WCGNNNNNNNCGW	1 cut(s)	684
PctI	GAATGC	1 cut(s)	1533
PdmI	GAANNNNTTC	1 cut(s)	2598
PfeI	GAWTC	9 cut(s)	8, 297, 536, 1418, 1645, 1962, 2081, 2203, 2660
PflMI	CCANNNNNTGG	2 cut(s)	204, 2570
PkrI	GCNGC	7 cut(s)	154, 157, 160, 163, 166, 169, 229
PleI	GAGTC	5 cut(s)	1382, 1459, 1582, 1735, 1841
PpsI	GAGTC	5 cut(s)	1382, 1459, 1582, 1735, 1841
PscI	ACATGT	1 cut(s)	2596
Psp124BI	GAGCTC	1 cut(s)	2047
Psp6I	CCWGG	1 cut(s)	1758
PspFI	CCCAGC	1 cut(s)	2008
PspGI	CCWGG	1 cut(s)	1758
PspN4I	GGNNCC	6 cut(s)	281, 1507, 2178, 2481, 2562, 2622
PspPI	GGNCC	7 cut(s)	1198, 1569, 1834, 2011, 2177, 2456, 2621
PspXI	VCTCGAGB	1 cut(s)	489
PsrI	GAACNNNNNNTAC	2 cut(s)	750, 782
PstI	CTGCAG	1 cut(s)	946
PstNI	CAGNNNCTG	1 cut(s)	1777
PsuI	RGATCY	2 cut(s)	733, 2560
RsaI	GTAC	3 cut(s)	722, 1588, 1688
RsaNI	GTAC	3 cut(s)	721, 1587, 1687
RseI	CAYNNNNRTG	1 cut(s)	2034
SacI	GAGCTC	1 cut(s)	2047
SacII	CCGCGG	1 cut(s)	295
SatI	GCNGC	7 cut(s)	153, 156, 159, 162, 165, 168, 228
Sau96I	GGNCC	7 cut(s)	1198, 1569, 1834, 2011, 2177, 2456, 2621
ScaI	AGTACT	1 cut(s)	1688
SchI	GAGTC	5 cut(s)	1383, 1460, 1583, 1736, 1841
ScrFI	CCNGG	1 cut(s)	1760
SduI	GDGCHC	3 cut(s)	1401, 2047, 2484
SfaNI	GCATC	9 cut(s)	132, 133, 587, 1194, 1377, 1624, 1901, 2499, 2618
SfcI	CTRYAG	1 cut(s)	942
Sfr274I	CTCGAG	1 cut(s)	489
Sfr303I	CCGCGG	1 cut(s)	295
SgrBI	CCGCGG	1 cut(s)	295
SinI	GGWCC	2 cut(s)	1198, 2621
SlaI	CTCGAG	1 cut(s)	489
SmiMI	CAYNNNNRTG	1 cut(s)	2034
SmlI	CTYRAG	5 cut(s)	137, 489, 1075, 1690, 2381
SmoI	CTYRAG	5 cut(s)	137, 489, 1075, 1690, 2381
SsiI	CCGC	5 cut(s)	292, 294, 783, 1412, 1959
SspI	AATATT	1 cut(s)	1620
SspMI	CTAG	6 cut(s)	408, 1223, 1277, 1325, 1362, 2087
SstI	GAGCTC	1 cut(s)	2047
StyD4I	CCNGG	1 cut(s)	1758
StyI	CCWWGG	2 cut(s)	1501, 1509
TaaI	ACNGT	6 cut(s)	194, 596, 709, 860, 1152, 1773
TaiI	ACGT	2 cut(s)	84, 722
TatI	WGTACW	2 cut(s)	1586, 1686
TfiI	GAWTC	9 cut(s)	8, 297, 536, 1418, 1645, 1962, 2081, 2203, 2660
TscAI	CASTG	6 cut(s)	199, 463, 615, 714, 1596, 2271
TseFI	GTSAC	3 cut(s)	184, 271, 1335
TseI	GCWGC	7 cut(s)	152, 155, 158, 161, 164, 167, 227
Tsp45I	GTSAC	3 cut(s)	184, 271, 1335
TspGWI	ACGGA	1 cut(s)	2047
TspRI	CASTG	6 cut(s)	199, 463, 615, 714, 1596, 2271
Van91I	CCANNNNNTGG	2 cut(s)	204, 2570
Vha464I	CTTAAG	2 cut(s)	1075, 1690
VpaK11BI	GGWCC	2 cut(s)	1198, 2621
XapI	RAATTY	5 cut(s)	968, 1122, 1423, 1893, 2235
XbaI	TCTAGA	1 cut(s)	2086
XceI	RCATGY	3 cut(s)	764, 2536, 2600
XcmI	CCANNNNNNNNNTGG	1 cut(s)	885
XhoI	CTCGAG	1 cut(s)	489
XmnI	GAANNNNTTC	1 cut(s)	2598
XspI	CTAG	6 cut(s)	408, 1223, 1277, 1325, 1362, 2087
ZrmI	AGTACT	1 cut(s)	1688
Zsp2I	ATGCAT	1 cut(s)	2323

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.