Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0003960

Physical Location & Seq

Genomic Coordinates Forward (+)

28532495 .. 28535538

Gene Structure 3044 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1186600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2835 bp

ATGGCAGAACAGATTATATGCTTTGTGATCGACAAGTTAATTCAACTACTCATCACCACAGAAGCAAACCTTTCGAGAAATGCAAGTACTGAAGTTGGTTTCATCAAAGATGAACTCGAGAGCATCCGATCATTCCTCAAGGAAGCAGATGCTAAGGCTGCAGCAGCAGACGGAGAGACGGGGAATGATGGTGTCAAAACTTGGGTCAATCAAGTAAGAGAAGCAGCTTTTTATATTGAAGATGTGATTGATGAATATTTGCTCCGCATCTTACAGCATCATCAACACCATGCATTCCTTCGTTCTTTCCACAAAATTATCCGGTTGGCTAAAAAATTGAGATCACAGGATGGGATAGTTTTGAAAGTTGACGGTATAAAAGAATTGGTTCGTGGAATCAAGGAGAGAAGTGAAAGATATGGATTCAAATCCCTTGATCAGCAAGGCCAGAGCAGTGGAGAAAACAACGACCCTCGAGTGGCTTCCCTTTTCATTGAGCAAGCTGAAATCGTGGGGATTGAATCTACCAGAGATGAGCTGACTGGTTTGTTGGTAGAAGAAGCACCAAGACGTAAAGTGATATCAGTGGTTGGCATGGGAGGACTTGGGAAAACCACTCTTGCCAAGAAAGTATATGACCAGCCGAAGGTGATGGCACACTTTGATTGCTATGCTTGGATTACGGTGTCCCAATCGTACAGGGTTGAGGATCTTCTGAGGACGGTAATAAAAAAGTTCTACATTTCAAGGAAGGAGCTTTTCCCAGAGGAAATCGACACAATGGATGAGGAGTCCCTGATTAGCACAGCAAGAGAGTATTTGCAGCAAAAGAGGTACGTTGTTGTGTTTGATGATGTGTGGAAAGTAGATTTTTGGGGGGCTATAGAACATGCTTTACCCGATAATAAAGGCGGACGAATAATTATCACTACACGGATTCAAGGTGTTGCCAACTTTTGCAGAAGATCTTGTTTTGTTCATGTTCATCACTTGCAGCATCTGCCTCCAAACAAGGCATGGGAATTGTTTTGCAGAAAGGCATTTCAGTTTGAATTGGAGGGAAAGTGTCCTGAAGATTTGGAAGAGTTGTCTCTTAACATCGTCAAAAGATGCGAAGGACTACCTTTGGCAATTGTTTCCATAGCTGGTCTGCTGTCAACCAAAGCTAAAGTTGTGTCTGAATGGCAGAAGTTATACAATAGCTTAAGTTCAGAGTTAGAAAGCAATCCCCACCTTACAAGCCTCACAAGAATCATATCCCTCAGCTATCACCATCTGCCGTATTACCTCAAATCGTGTGTATTATACTTTGGCATCTTTCCCGAGGATTACTCAGTTAGTTGCATAAGATTAGTTCGGTTGTGGATCGCTGAAGGTTTTGTGAAACCGAAGAGGGGCAAGACACTAGAAGAGGTTGGAGAGGAATACTTGATGGAGCTAATTCACAGAAGCCTGGTTCAAGTTTCAGAAGTTTACATTGATGGAAAAGCTCGAGGTTGTAGAGTTCATGATCTATTGCATGACGTCCTCCTTAAGAAGGGGTTGGATTCGAGCTTCTGTCATGTGTTATCAAAAGACGAGTCAACTTTTAAACCAACAACTCGACGTCTCTCAATGGACATCAGTCCTTCTGAGGCCGTGGGAAGCTTTACACAATCTCATATTCGTTCTGTATTTACTTTCAACCTAGAAGAATGGCCTGGGTCTTTTCTCGATACATTGAGTGGAAACATTAAACTTCTGAAAGTATTGGATTTCACAGATGCTCCAATCAATCAACTTCCGAAATATGTGGGGGATTTGTATCTCTTGACGTATTTAAGCTTGAGGAATACAAGGGTGGAGTTGCTTCCAGACTTCATAGGCAATCTACAAAACTTGGAAACCTTGGATCTAAAACAGTCTTTGGTGTATGAAATACCAGCCAAGATCAATAAGCTTGTTAAGCTGCGGCATCTTTTGGCCTACCATCGTGACTACAACTACGACGTGGGTTTGTATTGGAATTTGGAAAGAGGAGTGAAGATACATGAAGGTATTGGATGCTTACAAGCTTTGCAAAAATTGTCCTATGTGGAGGCGAATCATGGAGGGATCAGGTTAATCAAAGAATTGGGAAAGTTACGACAGCTACGGAAGTTAGGCCTAAAAAACTTGAAAAGTGAAGATGGAAGGGCTGTGTGTGCTTCTATTGAAAAGATGAACCACCTTGAATCTCTAATTTTAACTGCAATAAATGAAGATGAAGTCCTTGATCTAGAATCCATTTCAACTCCACCACAATTTATTCGTGCTCTCTTCTTGATAGGGCGTTTAGAGCAGTTGCCAGGTTGGATTTCAAATCTTCAACACCTCGTCAGATTGGTGATTTGTTGGTCAAGGTTGAGAGATTCCCCACTAAAAGCCCTTCAAAATCTACCTAATCTGTTGGAGCTAACTTTGGATATCAATGCATATGATGGTGTGCAGCTGCATTTTGAGGAAGGAGGATTTCATAAACTTAGAAAGCTACATCTCAGGGAAATGATGGGACTAAATTCGTTGATCATAGACGATGGTGTCATGCCTCTTCTCCAAGAGTTTCGCATTGGGCCTTCCCCACAAATGAATGAGGTACCCTCTGGCATCCACCATCTCCGGAATCTGACATATCTTCATTTCTATGACATGCCAAAGGAGTTTGCGCGACAGATGGATCCAAAAAATGGCCAACATTATTGGATTGTTGAACATATACAAAATGTTCGTTTTAGTTACAAGGTCGGACCAAGATGGCGTGTATATGAAATTCACGCCCTGCGTGATTCCAACTTTTCTCTTTGGTGGAATGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0002376 GO:0003674 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0008219 GO:0009626 GO:0009987 GO:0012501 GO:0016020 GO:0019897 GO:0019898 GO:0023052 GO:0033554 GO:0034050 GO:0036094 GO:0044425 GO:0044459 GO:0044464 GO:0045087 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071944 GO:0097159 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko04626 map04626

Pfam Domains

Protein Families

NB-ARC

Protein Analysis

945

Amino Acids

108.51

Weight (kDa)

6.94

Isoelectric Point (pI)

45.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Rx_N	PF18052	6 - 103	5e-20	Rx N-terminal domain
NB-ARC	PF00931	175 - 351	1.2e-41	NB-ARC domain
WHD_DRP	PF23559	439 - 509	3.2e-25	Disease resistance protein Winged helix domain
LRR_14	PF23598	554 - 886	9e-45	Leucine-rich repeat region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003960)

Species	Orthologous Gene IDs
malus_domestica	MD00G1097800.v1.1 MD01G1186500.v1.1 MD01G1186600.v1.1 MD01G1186800.v1.1 MD01G1187000.v1.1 MD01G1187100.v1.1 MD07G1258600.v1.1 MD07G1258700.v1.1 MD07G1258800.v1.1 MD07G1258900.v1.1 MD07G1259200.v1.1 MD07G1259300.v1.1
prunus_persica	Prupe.2G283200_v2.0.a1 Prupe.2G283300_v2.0.a1 Prupe.2G283400_v2.0.a1
pyrus_communis	pycom01g19810 pycom01g19820 pycom01g19840 pycom01g19900 pycom07g23170 pycom07g23230 pycom07g23240 pycom07g23250 pycom07g23260
rosa_chinensis	RchiOBHm_Chr1g0375241
rosa_laevigata	RLG00000026676
rosa_multiflora	Rmu_co8330553.1_g000001 Rmu_sc0000554.1_g000032
rosa_roxburghii	Rroxscaffold_4G00282910
rosa_rugosa	Rorug01G0389400
rosa_samantha	Rh1AG400100 Rh1BG364400 Rh1DG395000
rosa_wichuraiana	Rw1G035520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2558
AatII	GACGTC	2 cut(s)	1527, 1609
Acc65I	GGTACC	1 cut(s)	2614
AccB1I	GGYRCC	1 cut(s)	2614
AccB7I	CCANNNNNTGG	1 cut(s)	2705
AccII	CGCG	1 cut(s)	2686
AccIII	TCCGGA	1 cut(s)	2637
AciI	CCGC	3 cut(s)	265, 912, 1951
AclWI	GGATC	6 cut(s)	717, 1373, 1899, 2102, 2690, 2703
AcoI	YGGCCR	1 cut(s)	2707
AcsI	RAATTY	3 cut(s)	2005, 2536, 2787
AcuI	CTGAAG	3 cut(s)	111, 1092, 1392
AcyI	GRCGYC	2 cut(s)	1524, 1606
AfaI	GTAC	4 cut(s)	88, 698, 836, 2616
AfiI	CCNNNNNNNGG	5 cut(s)	478, 646, 1394, 1537, 2705
AflII	CTTAAG	2 cut(s)	1204, 1532
AjiI	CACGTC	1 cut(s)	1990
AjnI	CCWGG	3 cut(s)	1452, 1699, 2326
Alw21I	GWGCWC	1 cut(s)	2296
Alw26I	GTCTC	3 cut(s)	170, 1095, 1613
AlwI	GGATC	6 cut(s)	717, 1373, 1899, 2102, 2690, 2703
AlwNI	CAGNNNCTG	1 cut(s)	1000
Ama87I	CYCGRG	4 cut(s)	116, 474, 1322, 1491
Aor13HI	TCCGGA	1 cut(s)	2637
AoxI	GGCC	7 cut(s)	445, 1635, 1697, 1962, 2143, 2591, 2707
ApeKI	GCWGC	9 cut(s)	158, 161, 164, 224, 823, 994, 1948, 2467, 2470
ApoI	RAATTY	3 cut(s)	2005, 2536, 2787
ArsI	GACNNNNNNTTYG	4 cut(s)	189, 221, 1565, 1597
Asp700I	GAANNNNTTC	1 cut(s)	387
Asp718I	GGTACC	1 cut(s)	2614
AspLEI	GCGC	1 cut(s)	2686
AspS9I	GGNCC	2 cut(s)	2591, 2765
AsuHPI	GGTGA	4 cut(s)	46, 661, 1262, 2377
AvaI	CYCGRG	4 cut(s)	116, 474, 1322, 1491
AvaII	GGWCC	1 cut(s)	2765
BalI	TGGCCA	1 cut(s)	2709
BamHI	GGATCC	1 cut(s)	2695
BanI	GGYRCC	1 cut(s)	2614
Bbv12I	GWGCWC	1 cut(s)	2296
BbvCI	CCTCAGC	1 cut(s)	1262
BbvI	GCAGC	9 cut(s)	145, 173, 176, 236, 835, 1006, 1935, 2457, 2479
BceAI	ACGGC	2 cut(s)	1264, 1622
BcgI	CGANNNNNNTGC	2 cut(s)	54, 88
BciT130I	CCWGG	3 cut(s)	1454, 1701, 2328
BclI	TGATCA	2 cut(s)	436, 2544
BcoDI	GTCTC	3 cut(s)	170, 1095, 1613
BfaI	CTAG	3 cut(s)	1406, 1688, 2258
BfmI	CTRYAG	2 cut(s)	159, 882
BfrI	CTTAAG	2 cut(s)	1204, 1532
BglII	AGATCT	1 cut(s)	965
BmcAI	AGTACT	1 cut(s)	88
Bme1390I	CCNGG	3 cut(s)	1454, 1701, 2328
Bme18I	GGWCC	1 cut(s)	2765
BmeT110I	CYCGRG	4 cut(s)	116, 474, 1322, 1491
BmgBI	CACGTC	1 cut(s)	1990
BmgT120I	GGNCC	2 cut(s)	2591, 2765
BmiI	GGNNCC	2 cut(s)	2616, 2697
BmrFI	CCNGG	3 cut(s)	1454, 1701, 2328
BoxI	GACNNNNGTC	1 cut(s)	1623
BplI	GAGNNNNNCTC	4 cut(s)	1316, 1348, 2603, 2635
Bpu10I	CCTNAGC	2 cut(s)	153, 1262
BpuEI	CTTGAG	2 cut(s)	122, 1846
BsaBI	GATNNNNATC	2 cut(s)	427, 1803
BsaHI	GRCGYC	2 cut(s)	1524, 1606
BsaJI	CCNNGG	4 cut(s)	1323, 1638, 1700, 1887
BsaWI	WCCGGW	2 cut(s)	321, 2637
BsaXI	ACNNNNNCTCC	4 cut(s)	1049, 1079, 1750, 1780
Bsc4I	CCNNNNNNNGG	5 cut(s)	478, 646, 1394, 1537, 2705
Bse1I	ACTGG	1 cut(s)	547
Bse8I	GATNNNNATC	2 cut(s)	427, 1803
BseAI	TCCGGA	1 cut(s)	2637
BseBI	CCWGG	3 cut(s)	1454, 1701, 2328
BseDI	CCNNGG	4 cut(s)	1323, 1638, 1700, 1887
BseGI	GGATG	5 cut(s)	123, 355, 790, 2048, 2625
BseJI	GATNNNNATC	2 cut(s)	427, 1803
BseLI	CCNNNNNNNGG	5 cut(s)	478, 646, 1394, 1537, 2705
BseMII	CTCAG	5 cut(s)	707, 1276, 1347, 1623, 2530
BseNI	ACTGG	1 cut(s)	547
BseRI	GAGGAG	2 cut(s)	803, 2031
BseXI	GCAGC	9 cut(s)	145, 173, 176, 236, 835, 1006, 1935, 2457, 2479
BsgI	GTGCAG	1 cut(s)	2486
Bsh1236I	CGCG	1 cut(s)	2686
BshFI	GGCC	7 cut(s)	447, 1637, 1699, 1964, 2145, 2593, 2709
BshNI	GGYRCC	1 cut(s)	2614
BsiHKAI	GWGCWC	1 cut(s)	2296
BsiHKCI	CYCGRG	4 cut(s)	116, 474, 1322, 1491
BsiSI	CCGG	2 cut(s)	322, 2638
BslFI	GGGAC	3 cut(s)	673, 778, 2544
BslI	CCNNNNNNNGG	5 cut(s)	478, 646, 1394, 1537, 2705
BsmAI	GTCTC	3 cut(s)	170, 1095, 1613
BsmBI	CGTCTC	2 cut(s)	170, 1613
BsmFI	GGGAC	3 cut(s)	673, 778, 2544
BsmI	GAATGC	2 cut(s)	293, 2833
BsnI	GGCC	7 cut(s)	447, 1637, 1699, 1964, 2145, 2593, 2709
BsoBI	CYCGRG	4 cut(s)	116, 474, 1322, 1491
Bsp1286I	GDGCHC	1 cut(s)	2296
Bsp13I	TCCGGA	1 cut(s)	2637
BspACI	CCGC	3 cut(s)	265, 912, 1951
BspANI	GGCC	7 cut(s)	447, 1637, 1699, 1964, 2145, 2593, 2709
BspCNI	CTCAG	5 cut(s)	708, 1275, 1346, 1624, 2529
BspEI	TCCGGA	1 cut(s)	2637
BspFNI	CGCG	1 cut(s)	2686
BspHI	TCATGA	1 cut(s)	1507
BspLI	GGNNCC	2 cut(s)	2616, 2697
BspMAI	CTGCAG	1 cut(s)	163
BspPI	GGATC	6 cut(s)	717, 1373, 1899, 2102, 2690, 2703
BspT107I	GGYRCC	1 cut(s)	2614
BspTI	CTTAAG	2 cut(s)	1204, 1532
BsrI	ACTGG	1 cut(s)	547
BssECI	CCNNGG	4 cut(s)	1323, 1638, 1700, 1887
BssNI	GRCGYC	2 cut(s)	1524, 1606
BssT1I	CCWWGG	1 cut(s)	1887
Bst2UI	CCWGG	3 cut(s)	1454, 1701, 2328
Bst4CI	ACNGT	4 cut(s)	374, 685, 724, 1902
Bst6I	CTCTTC	5 cut(s)	1077, 1385, 1404, 2303, 2574
BstACI	GRCGYC	2 cut(s)	1524, 1606
BstAFI	CTTAAG	2 cut(s)	1204, 1532
BstAPI	GCANNNNNTGC	1 cut(s)	1000
BstC8I	GCNNGC	1 cut(s)	501
BstDEI	CTNAG	7 cut(s)	153, 716, 1262, 1333, 1632, 2501, 2516
BstDSI	CCRYGG	1 cut(s)	1638
BstENI	CCTNNNNNAGG	1 cut(s)	1535
BstF5I	GGATG	5 cut(s)	123, 355, 790, 2048, 2625
BstFNI	CGCG	1 cut(s)	2686
BstHHI	GCGC	1 cut(s)	2686
BstMAI	GTCTC	3 cut(s)	170, 1095, 1613
BstMWI	GCNNNNNNNGC	6 cut(s)	158, 164, 1000, 1945, 2183, 2317
BstNI	CCWGG	3 cut(s)	1454, 1701, 2328
BstNSI	RCATGY	2 cut(s)	893, 2671
BstPAI	GACNNNNGTC	1 cut(s)	1623
BstSCI	CCNGG	3 cut(s)	1452, 1699, 2326
BstSFI	CTRYAG	2 cut(s)	159, 882
BstUI	CGCG	1 cut(s)	2686
BstV1I	GCAGC	9 cut(s)	145, 173, 176, 236, 835, 1006, 1935, 2457, 2479
BstX2I	RGATCY	4 cut(s)	709, 965, 1891, 2695
BstXI	CCANNNNNNTGG	1 cut(s)	455
BstYI	RGATCY	4 cut(s)	709, 965, 1891, 2695
BsuRI	GGCC	7 cut(s)	447, 1637, 1699, 1964, 2145, 2593, 2709
BtgI	CCRYGG	1 cut(s)	1638
BtrI	CACGTC	1 cut(s)	1990
BtsCI	GGATG	5 cut(s)	123, 355, 790, 2048, 2625
BtsI	GCAGTG	1 cut(s)	460
BtsIMutI	CAGTG	2 cut(s)	460, 591
Cac8I	GCNNGC	1 cut(s)	501
CaiI	CAGNNNCTG	1 cut(s)	1000
CciI	TCATGA	1 cut(s)	1507
CfoI	GCGC	1 cut(s)	2686
Cfr13I	GGNCC	2 cut(s)	2591, 2765
Csp6I	GTAC	4 cut(s)	87, 697, 835, 2615
CviQI	GTAC	4 cut(s)	87, 697, 835, 2615
DdeI	CTNAG	7 cut(s)	153, 716, 1262, 1333, 1632, 2501, 2516
DraI	TTTAAA	1 cut(s)	1591
DrdI	GACNNNNNNGTC	1 cut(s)	2558
DseDI	GACNNNNNNGTC	1 cut(s)	2558
EaeI	YGGCCR	1 cut(s)	2707
Eam1104I	CTCTTC	5 cut(s)	1077, 1385, 1404, 2303, 2574
EarI	CTCTTC	5 cut(s)	1077, 1385, 1404, 2303, 2574
EciI	GGCGGA	1 cut(s)	927
Eco130I	CCWWGG	1 cut(s)	1887
Eco147I	AGGCCT	1 cut(s)	2145
Eco32I	GATATC	2 cut(s)	582, 2446
Eco47I	GGWCC	1 cut(s)	2765
Eco57I	CTGAAG	3 cut(s)	111, 1092, 1392
Eco88I	CYCGRG	4 cut(s)	116, 474, 1322, 1491
EcoNI	CCTNNNNNAGG	1 cut(s)	1535
EcoRII	CCWGG	3 cut(s)	1452, 1699, 2326
EcoRV	GATATC	2 cut(s)	582, 2446
EcoT14I	CCWWGG	1 cut(s)	1887
EcoT22I	ATGCAT	2 cut(s)	295, 2455
ErhI	CCWWGG	1 cut(s)	1887
Esp3I	CGTCTC	2 cut(s)	170, 1613
FalI	AAGNNNNNCTT	2 cut(s)	54, 86
FaqI	GGGAC	3 cut(s)	673, 778, 2544
FauNDI	CATATG	1 cut(s)	2455
FbaI	TGATCA	2 cut(s)	436, 2544
FokI	GGATG	5 cut(s)	110, 362, 797, 2055, 2612
FspBI	CTAG	3 cut(s)	1406, 1688, 2258
GlaI	GCGC	1 cut(s)	2685
HaeIII	GGCC	7 cut(s)	447, 1637, 1699, 1964, 2145, 2593, 2709
HapII	CCGG	2 cut(s)	322, 2638
HhaI	GCGC	1 cut(s)	2686
Hin1I	GRCGYC	2 cut(s)	1524, 1606
Hin6I	GCGC	1 cut(s)	2684
HinP1I	GCGC	1 cut(s)	2684
HincII	GTYRAC	3 cut(s)	370, 1158, 1584
HindII	GTYRAC	3 cut(s)	370, 1158, 1584
HindIII	AAGCTT	4 cut(s)	1645, 1822, 1937, 2052
HpaII	CCGG	2 cut(s)	322, 2638
HphI	GGTGA	4 cut(s)	46, 661, 1262, 2377
Hpy166II	GTNNAC	4 cut(s)	370, 1158, 1474, 1584
Hpy8I	GTNNAC	4 cut(s)	370, 1158, 1474, 1584
Hpy99I	CGWCG	2 cut(s)	1608, 1991
HpyCH4III	ACNGT	4 cut(s)	374, 685, 724, 1902
HpyCH4IV	ACGT	6 cut(s)	571, 837, 1524, 1606, 1814, 1989
HpyF10VI	GCNNNNNNNGC	6 cut(s)	158, 164, 1000, 1945, 2183, 2317
HpyF3I	CTNAG	7 cut(s)	153, 716, 1262, 1333, 1632, 2501, 2516
HpySE526I	ACGT	6 cut(s)	571, 837, 1524, 1606, 1814, 1989
Hsp92I	GRCGYC	2 cut(s)	1524, 1606
HspAI	GCGC	1 cut(s)	2684
Kpn2I	TCCGGA	1 cut(s)	2637
KpnI	GGTACC	1 cut(s)	2618
Ksp22I	TGATCA	2 cut(s)	436, 2544
LmnI	GCTCC	5 cut(s)	267, 754, 1435, 1771, 2431
Lsp1109I	GCAGC	9 cut(s)	145, 173, 176, 236, 835, 1006, 1935, 2457, 2479
MaeI	CTAG	3 cut(s)	1406, 1688, 2258
MaeII	ACGT	6 cut(s)	571, 837, 1524, 1606, 1814, 1989
MaeIII	GTNAC	3 cut(s)	1973, 2121, 2753
MfeI	CAATTG	1 cut(s)	1131
MflI	RGATCY	4 cut(s)	709, 965, 1891, 2695
MhlI	GDGCHC	1 cut(s)	2296
MlsI	TGGCCA	1 cut(s)	2709
MluNI	TGGCCA	1 cut(s)	2709
MlyI	GAGTC	2 cut(s)	800, 1589
MmeI	TCCRAC	6 cut(s)	1396, 1524, 2312, 2409, 2743, 2832
Mox20I	TGGCCA	1 cut(s)	2709
Mph1103I	ATGCAT	2 cut(s)	295, 2455
MroI	TCCGGA	1 cut(s)	2637
MroXI	GAANNNNTTC	1 cut(s)	387
MscI	TGGCCA	1 cut(s)	2709
MslI	CAYNNNNRTG	1 cut(s)	2661
Msp20I	TGGCCA	1 cut(s)	2709
MspA1I	CMGCKG	1 cut(s)	2470
MspCI	CTTAAG	2 cut(s)	1204, 1532
MspI	CCGG	2 cut(s)	322, 2638
MspR9I	CCNGG	3 cut(s)	1454, 1701, 2328
MunI	CAATTG	1 cut(s)	1131
Mva1269I	GAATGC	2 cut(s)	293, 2833
MvaI	CCWGG	3 cut(s)	1454, 1701, 2328
MvnI	CGCG	1 cut(s)	2686
MwoI	GCNNNNNNNGC	6 cut(s)	158, 164, 1000, 1945, 2183, 2317
NdeI	CATATG	1 cut(s)	2455
NlaIV	GGNNCC	2 cut(s)	2616, 2697
NmuCI	GTSAC	1 cut(s)	1973
NsiI	ATGCAT	2 cut(s)	295, 2455
NspI	RCATGY	2 cut(s)	893, 2671
PaeR7I	CTCGAG	3 cut(s)	116, 474, 1491
PagI	TCATGA	1 cut(s)	1507
PceI	AGGCCT	1 cut(s)	2145
PctI	GAATGC	2 cut(s)	293, 2833
PdmI	GAANNNNTTC	1 cut(s)	387
PflMI	CCANNNNNTGG	1 cut(s)	2705
PleI	GAGTC	2 cut(s)	799, 1588
PpsI	GAGTC	2 cut(s)	799, 1588
PshAI	GACNNNNGTC	1 cut(s)	1623
Psp6I	CCWGG	3 cut(s)	1452, 1699, 2326
PspGI	CCWGG	3 cut(s)	1452, 1699, 2326
PspN4I	GGNNCC	2 cut(s)	2616, 2697
PspPI	GGNCC	2 cut(s)	2591, 2765
PspXI	VCTCGAGB	2 cut(s)	474, 1491
PsrI	GAACNNNNNNTAC	2 cut(s)	879, 911
PstI	CTGCAG	1 cut(s)	163
PstNI	CAGNNNCTG	1 cut(s)	1000
PsuI	RGATCY	4 cut(s)	709, 965, 1891, 2695
PvuII	CAGCTG	1 cut(s)	2470
RsaI	GTAC	4 cut(s)	88, 698, 836, 2616
RsaNI	GTAC	4 cut(s)	87, 697, 835, 2615
RseI	CAYNNNNRTG	1 cut(s)	2661
Sau96I	GGNCC	2 cut(s)	2591, 2765
ScaI	AGTACT	1 cut(s)	88
SchI	GAGTC	2 cut(s)	800, 1589
ScrFI	CCNGG	3 cut(s)	1454, 1701, 2328
SduI	GDGCHC	1 cut(s)	2296
SfcI	CTRYAG	2 cut(s)	159, 882
Sfr274I	CTCGAG	3 cut(s)	116, 474, 1491
SinI	GGWCC	1 cut(s)	2765
SlaI	CTCGAG	3 cut(s)	116, 474, 1491
SmiMI	CAYNNNNRTG	1 cut(s)	2661
SmlI	CTYRAG	7 cut(s)	116, 137, 474, 1204, 1491, 1532, 1825
SmoI	CTYRAG	7 cut(s)	116, 137, 474, 1204, 1491, 1532, 1825
SseBI	AGGCCT	1 cut(s)	2145
SsiI	CCGC	3 cut(s)	265, 912, 1951
SspI	AATATT	1 cut(s)	257
SspMI	CTAG	3 cut(s)	1406, 1688, 2258
StuI	AGGCCT	1 cut(s)	2145
StyD4I	CCNGG	3 cut(s)	1452, 1699, 2326
StyI	CCWWGG	1 cut(s)	1887
TaaI	ACNGT	4 cut(s)	374, 685, 724, 1902
TaiI	ACGT	6 cut(s)	574, 840, 1527, 1609, 1817, 1992
TaqI	TCGA	9 cut(s)	30, 74, 117, 475, 774, 1492, 1550, 1603, 1713
TatI	WGTACW	1 cut(s)	86
TauI	GCSGC	1 cut(s)	1954
TscAI	CASTG	2 cut(s)	460, 591
TseFI	GTSAC	1 cut(s)	1973
TseI	GCWGC	9 cut(s)	158, 161, 164, 224, 823, 994, 1948, 2467, 2470
Tsp45I	GTSAC	1 cut(s)	1973
TspGWI	ACGGA	3 cut(s)	186, 949, 2149
TspRI	CASTG	2 cut(s)	460, 591
Van91I	CCANNNNNTGG	1 cut(s)	2705
Vha464I	CTTAAG	2 cut(s)	1204, 1532
VpaK11BI	GGWCC	1 cut(s)	2765
XagI	CCTNNNNNAGG	1 cut(s)	1535
XapI	RAATTY	3 cut(s)	2005, 2536, 2787
XbaI	TCTAGA	1 cut(s)	2257
XceI	RCATGY	2 cut(s)	893, 2671
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1014
XhoI	CTCGAG	3 cut(s)	116, 474, 1491
XmnI	GAANNNNTTC	1 cut(s)	387
XspI	CTAG	3 cut(s)	1406, 1688, 2258
ZraI	GACGTC	2 cut(s)	1525, 1607
ZrmI	AGTACT	1 cut(s)	88
Zsp2I	ATGCAT	2 cut(s)	295, 2455

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.