MD02G1056700.v1.1

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0005931
Physical Location & Seq
Reverse (-)
4582037 .. 4591195
9159 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1056700.v1.1.491

Sequence Viewer

Length: 360 bp
ATGGAATTGTTTGAGGCTACACAGAGCCAAATTCAGAAGCTAAAGCATCCCGCTTCTGCAGAATCGAGCAGTTCTCCAGACCGGCTGATCATGGCAGATTGTCATAACAATTCTGTTGGGGGAAAAGTATATACACCTCCCAAGGATTTTGTGTGTCCCATTACTAGCACTTTGTTTGATGATCCCGTGACACGGGGCAGACATATGAGCGAAAAGCGATCCAGGAGTGGATTGAAGGAGGGAACTCTACGTGCCCAATTACGCGCAAGAACTTGCACAGCACTCAACTGCCTAAGACCAACTATGTGCTCAAAAGGCTTATTGCAAGCTGGCAAGAACAGAATCCTAATGCAGTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
U-box WD40

Protein Analysis

120

Amino Acids

13.15

Weight (kDa)

9.54

Isoelectric Point (pI)

59.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 264
AciI CCGC 1 cut(s) 51
AclWI GGATC 2 cut(s) 176, 213
AcsI RAATTY 1 cut(s) 30
AfiI CCNNNNNNNGG 1 cut(s) 192
AgsI TTSAA 1 cut(s) 235
AjnI CCWGG 1 cut(s) 221
AluBI AGCT 2 cut(s) 40, 329
AluI AGCT 2 cut(s) 40, 329
Alw21I GWGCWC 1 cut(s) 311
AlwI GGATC 2 cut(s) 176, 213
ApoI RAATTY 1 cut(s) 30
AspLEI GCGC 1 cut(s) 266
BaeGI GKGCMC 1 cut(s) 256
Bbv12I GWGCWC 1 cut(s) 311
BciT130I CCWGG 1 cut(s) 223
BclI TGATCA 1 cut(s) 87
BfaI CTAG 1 cut(s) 165
BfmI CTRYAG 1 cut(s) 57
Bme1390I CCNGG 1 cut(s) 223
BmrFI CCNGG 1 cut(s) 223
BmsI GCATC 1 cut(s) 55
BplI GAGNNNNNCTC 2 cut(s) 58, 90
BpmI CTGGAG 1 cut(s) 60
BsaAI YACGTR 1 cut(s) 251
BsaJI CCNNGG 1 cut(s) 141
Bsc4I CCNNNNNNNGG 1 cut(s) 192
Bse118I RCCGGY 1 cut(s) 81
BseBI CCWGG 1 cut(s) 223
BseDI CCNNGG 1 cut(s) 141
BseGI GGATG 1 cut(s) 46
BseLI CCNNNNNNNGG 1 cut(s) 192
BseSI GKGCMC 1 cut(s) 256
Bsh1236I CGCG 1 cut(s) 264
BsiHKAI GWGCWC 1 cut(s) 311
BsiSI CCGG 1 cut(s) 82
BslFI GGGAC 1 cut(s) 141
BslI CCNNNNNNNGG 1 cut(s) 192
BsmFI GGGAC 1 cut(s) 141
Bsp1286I GDGCHC 2 cut(s) 256, 311
Bsp143I GATC 3 cut(s) 87, 181, 218
BspACI CCGC 1 cut(s) 51
BspFNI CGCG 1 cut(s) 264
BspMAI CTGCAG 1 cut(s) 61
BspPI GGATC 2 cut(s) 176, 213
BsrFI RCCGGY 1 cut(s) 81
BssAI RCCGGY 1 cut(s) 81
BssECI CCNNGG 1 cut(s) 141
BssMI GATC 3 cut(s) 87, 181, 218
BssT1I CCWWGG 1 cut(s) 141
Bst2UI CCWGG 1 cut(s) 223
BstBAI YACGTR 1 cut(s) 251
BstC8I GCNNGC 2 cut(s) 327, 331
BstDEI CTNAG 1 cut(s) 293
BstF5I GGATG 1 cut(s) 46
BstFNI CGCG 1 cut(s) 264
BstHHI GCGC 1 cut(s) 266
BstKTI GATC 3 cut(s) 90, 184, 221
BstMBI GATC 3 cut(s) 87, 181, 218
BstMWI GCNNNNNNNGC 1 cut(s) 315
BstNI CCWGG 1 cut(s) 223
BstSCI CCNGG 1 cut(s) 221
BstSFI CTRYAG 1 cut(s) 57
BstSLI GKGCMC 1 cut(s) 256
BstUI CGCG 1 cut(s) 264
BtsCI GGATG 1 cut(s) 46
BtsI GCAGTG 1 cut(s) 359
BtsIMutI CAGTG 1 cut(s) 359
Cac8I GCNNGC 2 cut(s) 327, 331
CfoI GCGC 1 cut(s) 266
Cfr10I RCCGGY 1 cut(s) 81
CviAII CATG 1 cut(s) 91
CviJI RGCY 6 cut(s) 17, 27, 40, 85, 318, 329
CviKI_1 RGCY 6 cut(s) 17, 27, 40, 85, 318, 329
DdeI CTNAG 1 cut(s) 293
DpnI GATC 3 cut(s) 89, 183, 220
DpnII GATC 3 cut(s) 87, 181, 218
Eco130I CCWWGG 1 cut(s) 141
EcoRII CCWGG 1 cut(s) 221
EcoT14I CCWWGG 1 cut(s) 141
ErhI CCWWGG 1 cut(s) 141
FaeI CATG 1 cut(s) 94
FaiI YATR 7 cut(s) 92, 105, 130, 132, 204, 206, 305
FaqI GGGAC 1 cut(s) 141
FatI CATG 1 cut(s) 90
FauI CCCGC 1 cut(s) 58
FauNDI CATATG 1 cut(s) 204
FbaI TGATCA 1 cut(s) 87
FokI GGATG 1 cut(s) 33
FspBI CTAG 1 cut(s) 165
GlaI GCGC 1 cut(s) 265
GsuI CTGGAG 1 cut(s) 60
HapII CCGG 1 cut(s) 82
HhaI GCGC 1 cut(s) 266
Hin1II CATG 1 cut(s) 94
Hin6I GCGC 1 cut(s) 264
HinP1I GCGC 1 cut(s) 264
HinfI GANTC 2 cut(s) 62, 342
HpaII CCGG 1 cut(s) 82
Hpy188I TCNGA 1 cut(s) 36
Hpy188III TCNNGA 1 cut(s) 77
HpyAV CCTTC 1 cut(s) 229
HpyCH4IV ACGT 1 cut(s) 250
HpyCH4V TGCA 4 cut(s) 59, 276, 325, 352
HpyF10VI GCNNNNNNNGC 1 cut(s) 315
HpyF3I CTNAG 1 cut(s) 293
HpySE526I ACGT 1 cut(s) 250
Hsp92II CATG 1 cut(s) 94
HspAI GCGC 1 cut(s) 264
Ksp22I TGATCA 1 cut(s) 87
Kzo9I GATC 3 cut(s) 87, 181, 218
LpnPI CCDG 5 cut(s) 90, 95, 208, 235, 315
LweI GCATC 1 cut(s) 55
MaeI CTAG 1 cut(s) 165
MaeII ACGT 1 cut(s) 250
MaeIII GTNAC 1 cut(s) 187
MalI GATC 3 cut(s) 89, 183, 220
MboI GATC 3 cut(s) 87, 181, 218
MhlI GDGCHC 2 cut(s) 256, 311
MluCI AATT 4 cut(s) 5, 30, 109, 257
MnlI CCTC 3 cut(s) 7, 147, 232
MspI CCGG 1 cut(s) 82
MspR9I CCNGG 1 cut(s) 223
MvaI CCWGG 1 cut(s) 223
MvnI CGCG 1 cut(s) 264
MwoI GCNNNNNNNGC 1 cut(s) 315
NdeI CATATG 1 cut(s) 204
NdeII GATC 3 cut(s) 87, 181, 218
NlaIII CATG 1 cut(s) 94
NmuCI GTSAC 1 cut(s) 187
PfeI GAWTC 2 cut(s) 62, 342
PfoI TCCNGGA 1 cut(s) 221
Ppu21I YACGTR 1 cut(s) 251
Psp6I CCWGG 1 cut(s) 221
PspGI CCWGG 1 cut(s) 221
PstI CTGCAG 1 cut(s) 61
Sau3AI GATC 3 cut(s) 87, 181, 218
ScrFI CCNGG 1 cut(s) 223
SduI GDGCHC 2 cut(s) 256, 311
SetI ASST 4 cut(s) 42, 139, 253, 331
SfaNI GCATC 1 cut(s) 55
SfcI CTRYAG 1 cut(s) 57
Sse9I AATT 4 cut(s) 5, 30, 109, 257
SsiI CCGC 1 cut(s) 51
SspMI CTAG 1 cut(s) 165
StyD4I CCNGG 1 cut(s) 221
StyI CCWWGG 1 cut(s) 141
TaiI ACGT 1 cut(s) 253
TaqI TCGA 1 cut(s) 65
TasI AATT 4 cut(s) 5, 30, 109, 257
TfiI GAWTC 2 cut(s) 62, 342
TscAI CASTG 1 cut(s) 359
TseFI GTSAC 1 cut(s) 187
Tsp45I GTSAC 1 cut(s) 187
TspRI CASTG 1 cut(s) 359
XapI RAATTY 1 cut(s) 30
XspI CTAG 1 cut(s) 165
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.