Rw4G014150

serine threonine-protein kinase cdc7

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0005931
Physical Location & Seq
Forward (+)
32975404 .. 32979236
3833 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G014150.1

Sequence Viewer

Length: 459 bp
ATGGGTGGCTCTGCTAGATCGGTCTGCTGCAGCTCCGTCCCTATTGCAGAGGCAATGGCTGTGTTGGACGGCATCAATTCTGCTTTGGAGCACAATTTCACAAAGGGTGTGGTGCACAGAGATATTAAGCCCGGAAACTTCCTCTTCTCTCAGAAGCTAAGCAAAGGTTACCTGATTGATTTCAACCTTGCCATGTTTGGAGGCCTTTTAACTACTGTTTTGGAGATCGTCTCCGAGAGCCTCCGCCTCTGCTTCACTGCCTGCGACACCGTCAACTGCACCGACTCCCTCTTTGACAATCTCAACGCCAGCCATGGAGCTGAGGACCGCAAGAAGGACCACCACCACAAAATCATCACCAGCGACTCTTATGCTGGAGACGACGGTAGCAAGGTGAAAAGCTTACCTGCTGTGCGATGTCGTTTCCGCGTTGTTCCCCATGACCGTGGTAAGGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000082 GO:0000278 GO:0000724 GO:0000725 GO:0000727 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005819 GO:0005856 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006270 GO:0006275 GO:0006281 GO:0006302 GO:0006310 GO:0006464 GO:0006468 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006897 GO:0006909 GO:0006950 GO:0006974 GO:0007049 GO:0007346 GO:0008150 GO:0008152 GO:0008284 GO:0008360 GO:0009058 GO:0009059 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010389 GO:0010556 GO:0010557 GO:0010564 GO:0010571 GO:0010604 GO:0010948 GO:0010971 GO:0015630 GO:0016192 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019219 GO:0019222 GO:0019538 GO:0022402 GO:0022603 GO:0022604 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0033262 GO:0033554 GO:0034641 GO:0034645 GO:0036211 GO:0042127 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044421 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044446 GO:0044464 GO:0044770 GO:0044772 GO:0044843 GO:0045171 GO:0045740 GO:0045786 GO:0045787 GO:0045931 GO:0045935 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051128 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065008 GO:0070013 GO:0070316 GO:0070317 GO:0071704 GO:0072686 GO:0080090 GO:0090068 GO:0090304 GO:0090329 GO:0098657 GO:0140096 GO:1901360 GO:1901564 GO:1901576 GO:1901987 GO:1901989 GO:1901990 GO:1901992 GO:1902749 GO:1902751 GO:1903047 GO:2000105 GO:2000112
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 map04110 map04111 map04113
Pfam Domains
Protein Families
Pkinase

Protein Analysis

152

Amino Acids

16.48

Weight (kDa)

7.67

Isoelectric Point (pI)

29.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 15 - 65 2.6e-07 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 415
AccII CGCG 1 cut(s) 429
AciI CCGC 3 cut(s) 244, 328, 427
AfiI CCNNNNNNNGG 2 cut(s) 334, 451
AgsI TTSAA 1 cut(s) 184
AluBI AGCT 4 cut(s) 33, 157, 320, 402
AluI AGCT 4 cut(s) 33, 157, 320, 402
Alw21I GWGCWC 2 cut(s) 93, 117
Alw26I GTCTC 2 cut(s) 235, 372
Alw44I GTGCAC 1 cut(s) 113
AoxI GGCC 1 cut(s) 202
ApaLI GTGCAC 1 cut(s) 113
ApeKI GCWGC 2 cut(s) 27, 30
ArsI GACNNNNNNTTYG 2 cut(s) 275, 307
AspS9I GGNCC 2 cut(s) 325, 337
AsuC2I CCSGG 1 cut(s) 132
AsuHPI GGTGA 2 cut(s) 349, 406
AvaII GGWCC 2 cut(s) 325, 337
BaeGI GKGCMC 1 cut(s) 117
Bbv12I GWGCWC 2 cut(s) 93, 117
BbvCI CCTCAGC 1 cut(s) 321
BbvI GCAGC 2 cut(s) 14, 42
BceAI ACGGC 1 cut(s) 85
BcgI CGANNNNNNTGC 2 cut(s) 353, 387
BcnI CCSGG 1 cut(s) 132
BcoDI GTCTC 2 cut(s) 235, 372
BfaI CTAG 1 cut(s) 15
BfmI CTRYAG 1 cut(s) 28
BfuAI ACCTGC 1 cut(s) 415
BisI GCNGC 2 cut(s) 28, 31
BlpI GCTNAGC 1 cut(s) 158
BlsI GCNGC 2 cut(s) 29, 32
Bme1390I CCNGG 1 cut(s) 132
Bme18I GGWCC 2 cut(s) 325, 337
BmgT120I GGNCC 2 cut(s) 325, 337
BmrFI CCNGG 1 cut(s) 132
BmsI GCATC 1 cut(s) 81
BplI GAGNNNNNCTC 2 cut(s) 215, 247
BpmI CTGGAG 1 cut(s) 396
Bpu10I CCTNAGC 1 cut(s) 321
Bpu1102I GCTNAGC 1 cut(s) 158
BpuMI CCSGG 1 cut(s) 132
BsaJI CCNNGG 2 cut(s) 313, 445
Bsc4I CCNNNNNNNGG 2 cut(s) 334, 451
Bse3DI GCAATG 1 cut(s) 60
BseDI CCNNGG 2 cut(s) 313, 445
BseLI CCNNNNNNNGG 2 cut(s) 334, 451
BseMI GCAATG 1 cut(s) 60
BseMII CTCAG 2 cut(s) 164, 312
BseSI GKGCMC 1 cut(s) 117
BseXI GCAGC 2 cut(s) 14, 42
BsgI GTGCAG 1 cut(s) 262
Bsh1236I CGCG 1 cut(s) 429
BshFI GGCC 1 cut(s) 204
BsiHKAI GWGCWC 2 cut(s) 93, 117
BsiSI CCGG 1 cut(s) 132
BslFI GGGAC 1 cut(s) 23
BslI CCNNNNNNNGG 2 cut(s) 334, 451
BsmAI GTCTC 2 cut(s) 235, 372
BsmBI CGTCTC 2 cut(s) 235, 372
BsmFI GGGAC 1 cut(s) 23
BsnI GGCC 1 cut(s) 204
Bsp1286I GDGCHC 2 cut(s) 93, 117
Bsp143I GATC 2 cut(s) 17, 225
Bsp1720I GCTNAGC 1 cut(s) 158
Bsp19I CCATGG 1 cut(s) 313
BspACI CCGC 3 cut(s) 244, 328, 427
BspANI GGCC 1 cut(s) 204
BspCNI CTCAG 2 cut(s) 163, 313
BspFNI CGCG 1 cut(s) 429
BspMAI CTGCAG 1 cut(s) 32
BspMI ACCTGC 1 cut(s) 415
BsrDI GCAATG 1 cut(s) 60
BssECI CCNNGG 2 cut(s) 313, 445
BssMI GATC 2 cut(s) 17, 225
BssT1I CCWWGG 1 cut(s) 313
Bst4CI ACNGT 4 cut(s) 217, 271, 386, 446
Bst6I CTCTTC 1 cut(s) 149
BstC8I GCNNGC 2 cut(s) 262, 310
BstDEI CTNAG 4 cut(s) 150, 158, 321, 456
BstDSI CCRYGG 2 cut(s) 313, 445
BstEII GGTNACC 1 cut(s) 167
BstFNI CGCG 1 cut(s) 429
BstKTI GATC 2 cut(s) 20, 228
BstMAI GTCTC 2 cut(s) 235, 372
BstMBI GATC 2 cut(s) 17, 225
BstPI GGTNACC 1 cut(s) 167
BstSCI CCNGG 1 cut(s) 130
BstSFI CTRYAG 1 cut(s) 28
BstSLI GKGCMC 1 cut(s) 117
BstUI CGCG 1 cut(s) 429
BstV1I GCAGC 2 cut(s) 14, 42
BstXI CCANNNNNNTGG 1 cut(s) 446
BsuRI GGCC 1 cut(s) 204
BtgI CCRYGG 2 cut(s) 313, 445
BtgZI GCGATG 1 cut(s) 430
BtsI GCAGTG 1 cut(s) 255
BtsIMutI CAGTG 1 cut(s) 255
BveI ACCTGC 1 cut(s) 415
Cac8I GCNNGC 2 cut(s) 262, 310
Cfr13I GGNCC 2 cut(s) 325, 337
CspCI CAANNNNNGTGG 2 cut(s) 90, 125
CviAII CATG 3 cut(s) 193, 314, 440
DdeI CTNAG 4 cut(s) 150, 158, 321, 456
DpnI GATC 2 cut(s) 19, 227
DpnII GATC 2 cut(s) 17, 225
Eam1104I CTCTTC 1 cut(s) 149
EarI CTCTTC 1 cut(s) 149
EciI GGCGGA 1 cut(s) 233
Eco130I CCWWGG 1 cut(s) 313
Eco147I AGGCCT 1 cut(s) 204
Eco47I GGWCC 2 cut(s) 325, 337
Eco91I GGTNACC 1 cut(s) 167
EcoO65I GGTNACC 1 cut(s) 167
EcoT14I CCWWGG 1 cut(s) 313
ErhI CCWWGG 1 cut(s) 313
Esp3I CGTCTC 2 cut(s) 235, 372
FaeI CATG 3 cut(s) 196, 317, 443
FaiI YATR 4 cut(s) 194, 315, 372, 441
FaqI GGGAC 1 cut(s) 23
FatI CATG 3 cut(s) 192, 313, 439
Fnu4HI GCNGC 2 cut(s) 28, 31
Fsp4HI GCNGC 2 cut(s) 28, 31
FspBI CTAG 1 cut(s) 15
GluI GCNGC 2 cut(s) 28, 31
GsuI CTGGAG 1 cut(s) 396
HaeIII GGCC 1 cut(s) 204
HapII CCGG 1 cut(s) 132
Hin1II CATG 3 cut(s) 196, 317, 443
HincII GTYRAC 1 cut(s) 274
HindII GTYRAC 1 cut(s) 274
HindIII AAGCTT 1 cut(s) 400
HinfI GANTC 2 cut(s) 284, 365
HpaII CCGG 1 cut(s) 132
HphI GGTGA 2 cut(s) 349, 406
Hpy166II GTNNAC 2 cut(s) 115, 274
Hpy188I TCNGA 2 cut(s) 153, 235
Hpy8I GTNNAC 2 cut(s) 115, 274
Hpy99I CGWCG 1 cut(s) 386
HpyAV CCTTC 1 cut(s) 328
HpyCH4III ACNGT 4 cut(s) 217, 271, 386, 446
HpyCH4V TGCA 4 cut(s) 30, 47, 115, 279
HpyF3I CTNAG 4 cut(s) 150, 158, 321, 456
Hsp92II CATG 3 cut(s) 196, 317, 443
Kzo9I GATC 2 cut(s) 17, 225
LmnI GCTCC 3 cut(s) 38, 88, 317
LpnPI CCDG 7 cut(s) 145, 185, 274, 322, 360, 373, 420
Lsp1109I GCAGC 2 cut(s) 14, 42
LweI GCATC 1 cut(s) 81
MaeI CTAG 1 cut(s) 15
MaeIII GTNAC 1 cut(s) 167
MalI GATC 2 cut(s) 19, 227
MboI GATC 2 cut(s) 17, 225
MboII GAAGA 1 cut(s) 136
MhlI GDGCHC 2 cut(s) 93, 117
MluCI AATT 2 cut(s) 76, 94
MlyI GAGTC 2 cut(s) 278, 359
MmeI TCCRAC 1 cut(s) 45
MnlI CCTC 7 cut(s) 43, 152, 194, 251, 257, 299, 316
MseI TTAA 2 cut(s) 126, 209
MslI CAYNNNNRTG 1 cut(s) 444
MspI CCGG 1 cut(s) 132
MspR9I CCNGG 1 cut(s) 132
MvnI CGCG 1 cut(s) 429
NciI CCSGG 1 cut(s) 132
NcoI CCATGG 1 cut(s) 313
NdeII GATC 2 cut(s) 17, 225
NlaIII CATG 3 cut(s) 196, 317, 443
PceI AGGCCT 1 cut(s) 204
PflFI GACNNNGTC 1 cut(s) 269
PkrI GCNGC 2 cut(s) 29, 32
PleI GAGTC 2 cut(s) 278, 359
PpsI GAGTC 2 cut(s) 278, 359
PspEI GGTNACC 1 cut(s) 167
PspPI GGNCC 2 cut(s) 325, 337
PstI CTGCAG 1 cut(s) 32
PsyI GACNNNGTC 1 cut(s) 269
RseI CAYNNNNRTG 1 cut(s) 444
SaqAI TTAA 2 cut(s) 126, 209
SatI GCNGC 2 cut(s) 28, 31
Sau3AI GATC 2 cut(s) 17, 225
Sau96I GGNCC 2 cut(s) 325, 337
SchI GAGTC 2 cut(s) 278, 359
ScrFI CCNGG 1 cut(s) 132
SduI GDGCHC 2 cut(s) 93, 117
SetI ASST 9 cut(s) 35, 159, 169, 174, 189, 322, 396, 404, 409
SfaNI GCATC 1 cut(s) 81
SfcI CTRYAG 1 cut(s) 28
SinI GGWCC 2 cut(s) 325, 337
SmiMI CAYNNNNRTG 1 cut(s) 444
Sse9I AATT 2 cut(s) 76, 94
SseBI AGGCCT 1 cut(s) 204
SsiI CCGC 3 cut(s) 244, 328, 427
SspMI CTAG 1 cut(s) 15
StuI AGGCCT 1 cut(s) 204
StyD4I CCNGG 1 cut(s) 130
StyI CCWWGG 1 cut(s) 313
TaaI ACNGT 4 cut(s) 217, 271, 386, 446
TaqII GACCGA 1 cut(s) 10
TasI AATT 2 cut(s) 76, 94
Tru1I TTAA 2 cut(s) 126, 209
Tru9I TTAA 2 cut(s) 126, 209
TscAI CASTG 1 cut(s) 262
TseI GCWGC 2 cut(s) 27, 30
TspGWI ACGGA 1 cut(s) 25
TspRI CASTG 1 cut(s) 262
Tth111I GACNNNGTC 1 cut(s) 269
VneI GTGCAC 1 cut(s) 113
VpaK11BI GGWCC 2 cut(s) 325, 337
XspI CTAG 1 cut(s) 15
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.