Rorug05G0109200

Phospholipase D

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0005931
Physical Location & Seq
Forward (+)
9701289 .. 9706013
4725 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0109200.1

Sequence Viewer

Length: 1416 bp
ATGGCGAGCAGCAGGAGAGCTTTGCAGAGTGTGTTGGGCTTCAACAGAGTGATCTGGACTGGACGGAGGAGCTTCGGGGCTGTTCCTCAAGGCAGTGCCTATTCATCTTCTCAGACCCTCATCAACTCTGAATATGAACACAGTGCCCACAATTACCACCCGATTCCAATTGTATTCTCTCAAGCGAAGGGATCATGCATATGGGATCCCGAGGGAAACAGATATCTGGACTTTCTTGCAGCTTACTCTGCTGTTAATCAGGGTCATTGTCATCCAAAGATCATGAAGGCATTACAAGAACAGGCAGAAAGGCTCACTCTTAGTTCCCGAGCTTTCTATAATGATAGATTTCCAGTATTTGCTGAGTATCTAACAAGCATGTTTGATTATGATATGGTGCTGCCTATGAACACTGGTGCGGAAGCTGTAGAAACAGCTTTAAAGTTGGCAAGGAAATGGGGTTATGAGAAGAAAAAAATTCCAAAAGATGAGGCTATTATTGTCTCATGTTGTGGCTGCTTCCATGGTCGTACATTGGCAGTCATTTCTATGAGCTGTGACAATGAGGCAACCCGAGGTTTTTGGCCCTTAGTGCCAGGACATCTTAAAGTTGATTTTGGTGATGCAGTTGGCCTTGAAAAAATCTTTAAAGAGAATGGAGATCGAATTGCTGGATTTCTTTTTGAACCCATTCAAGGAGAGGCTGGGGTTATAATTCCTCCAGATGGTTATTTGAAATCTGTCAGAGATCTATGCACAAAATATAACATTTTGATGATTGATGATGAAATACAATCTGGTTTAGCACGGTCAGGGAGAATGCTGGCTTGTGATTGGGAAGAAGTTCGCCCAGATGTAGTGATTCTAGGAAAAGCATTGGGTGGTGGTGTGATACCTGTAAGTGCGGTGCTTGCAGACAAAGACATTATGCTCTGTATCCGACCTGGAGAGCACGGAAGTACCTTTGGTGGTAATCCGTTGGCCAGTGCTGTTTCTATTGCCTCACTAGATGTAATAAGAGATGAGAGACTTGCTGAAAGATCTGCCCAAATGGGAGAAGAGCTGAGGAATCAGCTAAACAAGATTCAGCAGAAGTACCCAAACTACATTAAGGAAGTTCGAGGAAGAGGTTTGTTCAATGCTGTGGAGCTCAATAGCAAAAACTTGTCCCCTGTTTCTGCTTATGATATCTGTCTAAAGTTGAAGGAGAGAGGAGTTCTTGCCAAGCCAACTCATGATACTATTATCAGATTGACTCCTCCACTCTCCATAAGTTTGGAAGAGCTCGAAGAAGGATCAAAGGCAATCTCAGATGTTCTGGAACATGATCTACCTAAGATGCAGAAGGAAAAACCAGAAAACATCTCTTCAACTGCTTCCAACATATGTGACCGTTGTGGACGAAACTTGTACTGA

Protein Analysis

471

Amino Acids

52.15

Weight (kDa)

6.4

Isoelectric Point (pI)

39.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_3 PF00202 48 - 440 1.5e-123 Aminotransferase class-III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 713
AciI CCGC 2 cut(s) 419, 905
AclWI GGATC 4 cut(s) 199, 200, 213, 1303
AcoI YGGCCR 1 cut(s) 981
AcsI RAATTY 1 cut(s) 477
AfaI GTAC 4 cut(s) 532, 961, 1097, 1412
AfiI CCNNNNNNNGG 2 cut(s) 695, 725
AgsI TTSAA 8 cut(s) 43, 638, 686, 695, 736, 1138, 1204, 1371
AjnI CCWGG 2 cut(s) 595, 943
Alw21I GWGCWC 3 cut(s) 954, 1152, 1287
Alw26I GTCTC 2 cut(s) 508, 1021
AlwI GGATC 4 cut(s) 199, 200, 213, 1303
Ama87I CYCGRG 3 cut(s) 209, 327, 573
AoxI GGCC 3 cut(s) 584, 631, 981
ApeKI GCWGC 4 cut(s) 9, 239, 400, 516
ApoI RAATTY 1 cut(s) 477
Asp700I GAANNNNTTC 2 cut(s) 690, 843
AspS9I GGNCC 1 cut(s) 585
AsuHPI GGTGA 1 cut(s) 632
AvaI CYCGRG 3 cut(s) 209, 327, 573
BaeGI GKGCMC 1 cut(s) 148
BaeI ACNNNNGTAYC 2 cut(s) 943, 976
BalI TGGCCA 1 cut(s) 983
BamHI GGATCC 1 cut(s) 205
BanII GRGCYC 2 cut(s) 1152, 1287
Bbv12I GWGCWC 3 cut(s) 954, 1152, 1287
BbvCI CCTCAGC 1 cut(s) 1064
BbvI GCAGC 4 cut(s) 21, 251, 387, 503
BccI CCATC 1 cut(s) 719
BciT130I CCWGG 2 cut(s) 597, 945
BciVI GTATCC 1 cut(s) 947
BcoDI GTCTC 2 cut(s) 508, 1021
BfaI CTAG 2 cut(s) 866, 1007
BfmI CTRYAG 1 cut(s) 426
BfuI GTATCC 1 cut(s) 947
BglII AGATCT 2 cut(s) 748, 1040
BisI GCNGC 4 cut(s) 10, 240, 401, 517
BlsI GCNGC 4 cut(s) 11, 241, 402, 518
Bme1390I CCNGG 2 cut(s) 597, 945
BmeT110I CYCGRG 3 cut(s) 209, 327, 573
BmgT120I GGNCC 1 cut(s) 585
BmiI GGNNCC 1 cut(s) 207
BmrFI CCNGG 2 cut(s) 597, 945
BmsI GCATC 2 cut(s) 613, 1329
BpmI CTGGAG 2 cut(s) 705, 966
Bpu10I CCTNAGC 1 cut(s) 1064
BpuEI CTTGAG 2 cut(s) 72, 165
BsaJI CCNNGG 3 cut(s) 210, 523, 574
Bsc4I CCNNNNNNNGG 2 cut(s) 695, 725
Bse1I ACTGG 4 cut(s) 64, 353, 418, 984
BseBI CCWGG 2 cut(s) 597, 945
BseDI CCNNGG 3 cut(s) 210, 523, 574
BseGI GGATG 1 cut(s) 271
BseLI CCNNNNNNNGG 2 cut(s) 695, 725
BseMII CTCAG 4 cut(s) 125, 354, 1055, 1323
BseNI ACTGG 4 cut(s) 64, 353, 418, 984
BseRI GAGGAG 3 cut(s) 82, 1227, 1248
BseSI GKGCMC 1 cut(s) 148
BseXI GCAGC 4 cut(s) 21, 251, 387, 503
BseYI CCCAGC 1 cut(s) 704
BshFI GGCC 3 cut(s) 586, 633, 983
BsiHKAI GWGCWC 3 cut(s) 954, 1152, 1287
BsiHKCI CYCGRG 3 cut(s) 209, 327, 573
BslFI GGGAC 1 cut(s) 1153
BslI CCNNNNNNNGG 2 cut(s) 695, 725
BsmAI GTCTC 2 cut(s) 508, 1021
BsmFI GGGAC 1 cut(s) 1153
BsmI GAATGC 1 cut(s) 825
BsnI GGCC 3 cut(s) 586, 633, 983
BsoBI CYCGRG 3 cut(s) 209, 327, 573
Bsp1286I GDGCHC 4 cut(s) 148, 954, 1152, 1287
Bsp143I GATC 9 cut(s) 51, 191, 205, 279, 661, 748, 1040, 1295, 1327
Bsp19I CCATGG 1 cut(s) 523
BspACI CCGC 2 cut(s) 419, 905
BspANI GGCC 3 cut(s) 586, 633, 983
BspCNI CTCAG 4 cut(s) 124, 355, 1056, 1322
BspHI TCATGA 2 cut(s) 282, 1234
BspLI GGNNCC 1 cut(s) 207
BspPI GGATC 4 cut(s) 199, 200, 213, 1303
BspQI GCTCTTC 2 cut(s) 1053, 1275
BsrI ACTGG 4 cut(s) 64, 353, 418, 984
BssECI CCNNGG 3 cut(s) 210, 523, 574
BssMI GATC 9 cut(s) 51, 191, 205, 279, 661, 748, 1040, 1295, 1327
BssT1I CCWWGG 1 cut(s) 523
Bst2UI CCWGG 2 cut(s) 597, 945
Bst4CI ACNGT 3 cut(s) 143, 810, 1394
Bst6I CTCTTC 4 cut(s) 1053, 1120, 1275, 1372
BstC8I GCNNGC 3 cut(s) 7, 825, 912
BstDEI CTNAG 7 cut(s) 111, 320, 363, 589, 1064, 1309, 1335
BstDSI CCRYGG 1 cut(s) 523
BstF5I GGATG 1 cut(s) 271
BstKTI GATC 9 cut(s) 54, 194, 208, 282, 664, 751, 1043, 1298, 1330
BstMAI GTCTC 2 cut(s) 508, 1021
BstMBI GATC 9 cut(s) 51, 191, 205, 279, 661, 748, 1040, 1295, 1327
BstMWI GCNNNNNNNGC 3 cut(s) 248, 592, 911
BstNI CCWGG 2 cut(s) 597, 945
BstNSI RCATGY 1 cut(s) 382
BstSCI CCNGG 2 cut(s) 595, 943
BstSFI CTRYAG 1 cut(s) 426
BstSLI GKGCMC 1 cut(s) 148
BstV1I GCAGC 4 cut(s) 21, 251, 387, 503
BstX2I RGATCY 3 cut(s) 205, 748, 1040
BstXI CCANNNNNNTGG 1 cut(s) 1276
BstYI RGATCY 3 cut(s) 205, 748, 1040
BsuI GTATCC 1 cut(s) 947
BsuRI GGCC 3 cut(s) 586, 633, 983
BtgI CCRYGG 1 cut(s) 523
BtsCI GGATG 1 cut(s) 271
BtsI GCAGTG 1 cut(s) 100
BtsIMutI CAGTG 4 cut(s) 100, 148, 411, 991
Cac8I GCNNGC 3 cut(s) 7, 825, 912
CciI TCATGA 2 cut(s) 282, 1234
Cfr13I GGNCC 1 cut(s) 585
Csp6I GTAC 4 cut(s) 531, 960, 1096, 1411
CviAII CATG 7 cut(s) 195, 283, 379, 507, 524, 1235, 1325
CviQI GTAC 4 cut(s) 531, 960, 1096, 1411
DdeI CTNAG 7 cut(s) 111, 320, 363, 589, 1064, 1309, 1335
DpnI GATC 9 cut(s) 53, 193, 207, 281, 663, 750, 1042, 1297, 1329
DpnII GATC 9 cut(s) 51, 191, 205, 279, 661, 748, 1040, 1295, 1327
DraI TTTAAA 2 cut(s) 441, 649
EaeI YGGCCR 1 cut(s) 981
Eam1104I CTCTTC 4 cut(s) 1053, 1120, 1275, 1372
EarI CTCTTC 4 cut(s) 1053, 1120, 1275, 1372
Ecl136II GAGCTC 2 cut(s) 1150, 1285
Eco130I CCWWGG 1 cut(s) 523
Eco24I GRGCYC 2 cut(s) 1152, 1287
Eco32I GATATC 2 cut(s) 224, 1189
Eco53kI GAGCTC 2 cut(s) 1150, 1285
Eco88I CYCGRG 3 cut(s) 209, 327, 573
EcoICRI GAGCTC 2 cut(s) 1150, 1285
EcoRII CCWGG 2 cut(s) 595, 943
EcoRV GATATC 2 cut(s) 224, 1189
EcoT14I CCWWGG 1 cut(s) 523
EcoT22I ATGCAT 1 cut(s) 200
EcoT38I GRGCYC 2 cut(s) 1152, 1287
ErhI CCWWGG 1 cut(s) 523
FaeI CATG 7 cut(s) 198, 286, 382, 510, 527, 1238, 1328
FaqI GGGAC 1 cut(s) 1153
FatI CATG 7 cut(s) 194, 282, 378, 506, 523, 1234, 1324
FauNDI CATATG 2 cut(s) 200, 1385
Fnu4HI GCNGC 4 cut(s) 10, 240, 401, 517
FokI GGATG 1 cut(s) 258
FriOI GRGCYC 2 cut(s) 1152, 1287
Fsp4HI GCNGC 4 cut(s) 10, 240, 401, 517
FspBI CTAG 2 cut(s) 866, 1007
GluI GCNGC 4 cut(s) 10, 240, 401, 517
GsaI CCCAGC 1 cut(s) 708
GsuI CTGGAG 2 cut(s) 705, 966
HaeIII GGCC 3 cut(s) 586, 633, 983
Hin1II CATG 7 cut(s) 198, 286, 382, 510, 527, 1238, 1328
HinfI GANTC 5 cut(s) 163, 862, 1069, 1084, 1255
HphI GGTGA 1 cut(s) 632
Hpy166II GTNNAC 1 cut(s) 1400
Hpy188I TCNGA 6 cut(s) 114, 130, 746, 941, 1250, 1312
Hpy188III TCNNGA 8 cut(s) 55, 209, 227, 283, 327, 722, 1235, 1319
Hpy8I GTNNAC 1 cut(s) 1400
HpyAV CCTTC 5 cut(s) 181, 280, 1198, 1286, 1339
HpyCH4III ACNGT 3 cut(s) 143, 810, 1394
HpyCH4V TGCA 7 cut(s) 25, 198, 239, 626, 756, 914, 1342
HpyF10VI GCNNNNNNNGC 3 cut(s) 248, 592, 911
HpyF3I CTNAG 7 cut(s) 111, 320, 363, 589, 1064, 1309, 1335
Hsp92II CATG 7 cut(s) 198, 286, 382, 510, 527, 1238, 1328
Kzo9I GATC 9 cut(s) 51, 191, 205, 279, 661, 748, 1040, 1295, 1327
LguI GCTCTTC 2 cut(s) 1053, 1275
LmnI GCTCC 2 cut(s) 69, 1147
Lsp1109I GCAGC 4 cut(s) 21, 251, 387, 503
LweI GCATC 2 cut(s) 613, 1329
MaeI CTAG 2 cut(s) 866, 1007
MaeIII GTNAC 2 cut(s) 557, 1388
MalI GATC 9 cut(s) 53, 193, 207, 281, 663, 750, 1042, 1297, 1329
MboI GATC 9 cut(s) 51, 191, 205, 279, 661, 748, 1040, 1295, 1327
MboII GAAGA 8 cut(s) 99, 481, 851, 1070, 1137, 1292, 1301, 1359
MfeI CAATTG 1 cut(s) 168
MflI RGATCY 3 cut(s) 205, 748, 1040
MhlI GDGCHC 4 cut(s) 148, 954, 1152, 1287
MlsI TGGCCA 1 cut(s) 983
MluCI AATT 5 cut(s) 151, 168, 477, 666, 714
MluNI TGGCCA 1 cut(s) 983
MlyI GAGTC 1 cut(s) 1249
MmeI TCCRAC 2 cut(s) 964, 1404
Mox20I TGGCCA 1 cut(s) 983
Mph1103I ATGCAT 1 cut(s) 200
MroXI GAANNNNTTC 2 cut(s) 690, 843
MscI TGGCCA 1 cut(s) 983
MseI TTAA 5 cut(s) 255, 440, 606, 648, 1110
MslI CAYNNNNRTG 3 cut(s) 199, 548, 773
Msp20I TGGCCA 1 cut(s) 983
MspR9I CCNGG 2 cut(s) 597, 945
MunI CAATTG 1 cut(s) 168
Mva1269I GAATGC 1 cut(s) 825
MvaI CCWGG 2 cut(s) 597, 945
MwoI GCNNNNNNNGC 3 cut(s) 248, 592, 911
NcoI CCATGG 1 cut(s) 523
NdeI CATATG 2 cut(s) 200, 1385
NdeII GATC 9 cut(s) 51, 191, 205, 279, 661, 748, 1040, 1295, 1327
NlaIII CATG 7 cut(s) 198, 286, 382, 510, 527, 1238, 1328
NlaIV GGNNCC 1 cut(s) 207
NmuCI GTSAC 2 cut(s) 557, 1388
NsiI ATGCAT 1 cut(s) 200
NspI RCATGY 1 cut(s) 382
PagI TCATGA 2 cut(s) 282, 1234
PciSI GCTCTTC 2 cut(s) 1053, 1275
PctI GAATGC 1 cut(s) 825
PdmI GAANNNNTTC 2 cut(s) 690, 843
PfeI GAWTC 4 cut(s) 163, 862, 1069, 1084
PkrI GCNGC 4 cut(s) 11, 241, 402, 518
PleI GAGTC 1 cut(s) 1249
PpsI GAGTC 1 cut(s) 1249
PsiI TTATAA 1 cut(s) 713
Psp124BI GAGCTC 2 cut(s) 1152, 1287
Psp6I CCWGG 2 cut(s) 595, 943
PspFI CCCAGC 1 cut(s) 704
PspGI CCWGG 2 cut(s) 595, 943
PspN4I GGNNCC 1 cut(s) 207
PspPI GGNCC 1 cut(s) 585
PsrI GAACNNNNNNTAC 2 cut(s) 1314, 1346
PsuI RGATCY 3 cut(s) 205, 748, 1040
RsaI GTAC 4 cut(s) 532, 961, 1097, 1412
RsaNI GTAC 4 cut(s) 531, 960, 1096, 1411
RseI CAYNNNNRTG 3 cut(s) 199, 548, 773
SacI GAGCTC 2 cut(s) 1152, 1287
SapI GCTCTTC 2 cut(s) 1053, 1275
SaqAI TTAA 5 cut(s) 255, 440, 606, 648, 1110
SatI GCNGC 4 cut(s) 10, 240, 401, 517
Sau3AI GATC 9 cut(s) 51, 191, 205, 279, 661, 748, 1040, 1295, 1327
Sau96I GGNCC 1 cut(s) 585
SchI GAGTC 1 cut(s) 1249
ScrFI CCNGG 2 cut(s) 597, 945
SduI GDGCHC 4 cut(s) 148, 954, 1152, 1287
SfaNI GCATC 2 cut(s) 613, 1329
SfcI CTRYAG 1 cut(s) 426
SmiMI CAYNNNNRTG 3 cut(s) 199, 548, 773
SmlI CTYRAG 2 cut(s) 87, 180
SmoI CTYRAG 2 cut(s) 87, 180
Sse9I AATT 5 cut(s) 151, 168, 477, 666, 714
SsiI CCGC 2 cut(s) 419, 905
SspMI CTAG 2 cut(s) 866, 1007
SstI GAGCTC 2 cut(s) 1152, 1287
StyD4I CCNGG 2 cut(s) 595, 943
StyI CCWWGG 1 cut(s) 523
TaaI ACNGT 3 cut(s) 143, 810, 1394
TaqI TCGA 3 cut(s) 664, 1120, 1287
TasI AATT 5 cut(s) 151, 168, 477, 666, 714
TatI WGTACW 1 cut(s) 1410
TfiI GAWTC 4 cut(s) 163, 862, 1069, 1084
Tru1I TTAA 5 cut(s) 255, 440, 606, 648, 1110
Tru9I TTAA 5 cut(s) 255, 440, 606, 648, 1110
TscAI CASTG 4 cut(s) 100, 148, 418, 991
TseFI GTSAC 2 cut(s) 557, 1388
TseI GCWGC 4 cut(s) 9, 239, 400, 516
Tsp45I GTSAC 2 cut(s) 557, 1388
TspDTI ATGAA 5 cut(s) 93, 150, 299, 422, 801
TspGWI ACGGA 3 cut(s) 79, 966, 969
TspRI CASTG 4 cut(s) 100, 148, 418, 991
XapI RAATTY 1 cut(s) 477
XceI RCATGY 1 cut(s) 382
XmnI GAANNNNTTC 2 cut(s) 690, 843
XspI CTAG 2 cut(s) 866, 1007
Zsp2I ATGCAT 1 cut(s) 200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.