Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0003495

Physical Location & Seq

Genomic Coordinates Reverse (-)

5904355 .. 5908568

Gene Structure 4214 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1073400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1578 bp

ATGTGGCAGTATATATTGAAAACTAGTGTGTACCCAAGAGAACCAAATGCTCTCAAGGAACTGAGGATTGCTGCTTCAAACCACCCCATGGCTTTCATGAGCACTTCACCGGATTCCGGTCAGTTCATGGCCCTTCTCTTGAAGCTGGTGAATCCAAAGAAGGCAATTGAAATCGGAGTTTTTACCGGCTACTCTCTTCTCCTCACGGCTCTCACAATTCCTGATGATGGCAAGATTACGGCCATAGACACCGACCGAAAAGCATACGAAATGGGGTTGCCAAGTATAAAAAAAGCTGGCGTCGAAAACAAAATCGACTTCATTGAGTCCCCAGCTATGCCAATTCTTGATAAACTCTTAGAAGACCCAGAGAAGGAAGGGAGTTTCGACTTTGCTTTCGTTGACGCGGACAAGAACAACTACTGGAACTACCACGAAAGGCTAATGAAACTGATTAAGGTTGGCGGGATGGTAATGTATGATAACACACTCTGGGGAGGAACAGTTGCTTGGCCGGAAGAGGATGCTCCGGAGGCCAAAAGGGAGTGGCGGCGGTGTGCAATTGAGTTTAATGAATTGGTTTCCGCTGACGCTCGCGTCGAAATTTCCCAGCTCATGCCAAAGACAAAGGCAGTACAACTTTCCTCAACAGCGGTTGGTGGAATATATGTCAGCAGCTTGGTGGGTGGGTCGGGTGCCACGGGAGAAATCCCCAACTCATCAAAAACATATTTATTTAGAGAGGTGTTGATTCGGTTCACCATCGCTGCTGCAAATTCAAAGTCCAAAGACGGAAGACTCGGCCTTCACCTTGGATCATCGGAATTGCTTGGACTAGTTGCTTTCTCTTTGCAAATGGAGCACAGCGCCAAAAAGGACCTGAATCTAGAGAAGAAGGGACTGCTGCAGAGTGACGAGTTATATCAGTATATCTTGGAGACTAGTGTGTATCCACATGAACCGGAGCCTCTCAAGGAGCTTAGAGCTGCCACCGCCAGCCACCCGAGGGCTGAGATGGCTACTGCACCAGATGCAGGTCAGTTAATGGCCATGCTGTTGAAGCTAGTAGATGCAAAGAAAACTATTGAAGTTGGCGTTTTCACCGGATACTCTCTCCTCCTCACTGCTCTTACAATTCCTGACGATGGCAAGATTGTAGCCATAGATGTGAATCGTGAGACGTATGAGCAAATAGGGCTTCCAATCATAAAGAAAGCCGGTGTTGAGCACAAAATCGACTTCATTGAATCCCAGGCCCTACCGGTTCTTGATAAGCTACTAGAAAATCATGAAAACGAAGGAAGTTTCGACTTTGCTTTCATCGATGCCGACAAGGTCAACTACTGGAACTACCATGAGAGGTTAATGAAGCTGTTGAAGGTGGGTGGTATAGTTGTCTATGATAACACCCTCTGGGGAGGAACGGTTGTAATGCCTGATGAGTTGATTCCGGAGTTCAGGAAACCAGGCAAACAGGCGACAATCGAGCTTAACAAGTTACTTGCAGCCGATCCTCGTGTCCAGATTTCACATGCTTCTTTGGGTGATGGGATCACAATTTGTCGTCGACTTTACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0008150 GO:0008152 GO:0008168 GO:0008171 GO:0008757 GO:0016740 GO:0016741 GO:0032259 GO:0042409 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00360 ko00940 ko00941 ko00945 ko01100 ko01110 map00360 map00940 map00941 map00945 map01100 map01110

Pfam Domains

Protein Families

Methyltransf_3

Protein Analysis

526

Amino Acids

58.28

Weight (kDa)

5.49

Isoelectric Point (pI)

32.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Methyltransf_3	PF01596	8 - 205	1.8e-69	O-methyltransferase
Methyltransf_24	PF13578	56 - 162	9.5e-09	Methyltransferase domain
Methyltransf_3	PF01596	313 - 524	1.8e-77	O-methyltransferase
Methyltransf_24	PF13578	362 - 469	9.6e-09	Methyltransferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003495)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G26220
fragaria_vesca	FvH4_1g06840 FvH4_1g06841 FvH4_1g06841
malus_domestica	MD02G1073100.v1.1 MD02G1073400.v1.1
prunus_persica	Prupe.7G214400_v2.0.a1 Prupe.7G214400_v2.0.a1 Prupe.7G214400_v2.0.a1
pyrus_communis	pycom02g05730 pycom02g05760 pycom02g05770
rosa_chinensis	RchiOBHm_Chr2g0092621 RchiOBHm_Chr2g0092631 RchiOBHm_Chr2g0093901 RchiOBHm_Chr2g0093911
rosa_laevigata	RLG00000016207 RLG00000016329
rosa_multiflora	Rmu_co8197646.1_g000001 Rmu_co8235837.1_g000001 Rmu_co8321945.1_g000001 Rmu_sc0003027.1_g000003
rosa_roxburghii	Rroxscaffold_2G00147620 Rroxscaffold_2G00147650 Rroxscaffold_2G00148920
rosa_rugosa	Rorug02G0028200 Rorug02G0028300
rosa_samantha	Rh2AG074000 Rh2AG074100 Rh2BG075100 Rh2BG075200 Rh2CG076700 Rh2CG076800
rosa_wichuraiana	Rw2G005890 Rw2G005900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	596
Acc36I	ACCTGC	1 cut(s)	1025
AccB1I	GGYRCC	1 cut(s)	695
AccB7I	CCANNNNNTGG	1 cut(s)	88
AccI	GTMKAC	1 cut(s)	1567
AccII	CGCG	2 cut(s)	407, 597
AccIII	TCCGGA	2 cut(s)	529, 1450
AciI	CCGC	7 cut(s)	407, 465, 550, 553, 585, 653, 993
AclWI	GGATC	3 cut(s)	823, 1505, 1559
AcoI	YGGCCR	3 cut(s)	240, 512, 1047
AcsI	RAATTY	2 cut(s)	603, 775
AcyI	GRCGYC	1 cut(s)	300
AfaI	GTAC	2 cut(s)	32, 636
AfiI	CCNNNNNNNGG	7 cut(s)	88, 116, 227, 373, 1006, 1034, 1145
AgeI	ACCGGT	1 cut(s)	1261
AgsI	TTSAA	9 cut(s)	19, 78, 142, 170, 780, 1060, 1088, 1247, 1378
AhlI	ACTAGT	3 cut(s)	23, 835, 941
AjnI	CCWGG	2 cut(s)	1251, 1465
AjuI	GAANNNNNNNTTGG	2 cut(s)	493, 525
Alw21I	GWGCWC	3 cut(s)	104, 864, 1230
Alw26I	GTCTC	2 cut(s)	932, 1172
AlwI	GGATC	3 cut(s)	823, 1505, 1559
Ama87I	CYCGRG	1 cut(s)	1003
Aor13HI	TCCGGA	2 cut(s)	529, 1450
AoxI	GGCC	7 cut(s)	129, 240, 512, 534, 802, 1047, 1254
ApeKI	GCWGC	7 cut(s)	71, 675, 767, 770, 904, 986, 1505
ApoI	RAATTY	2 cut(s)	603, 775
ArsI	GACNNNNNNTTYG	4 cut(s)	380, 412, 767, 799
AsiGI	ACCGGT	1 cut(s)	1261
AspLEI	GCGC	1 cut(s)	869
AspS9I	GGNCC	3 cut(s)	130, 877, 1255
AsuHPI	GGTGA	6 cut(s)	99, 160, 751, 800, 1093, 1556
AvaI	CYCGRG	1 cut(s)	1003
AvaII	GGWCC	1 cut(s)	877
BalI	TGGCCA	1 cut(s)	1049
BanI	GGYRCC	1 cut(s)	695
BauI	CACGAG	1 cut(s)	1515
BbsI	GAAGAC	2 cut(s)	369, 802
Bbv12I	GWGCWC	3 cut(s)	104, 864, 1230
BbvI	GCAGC	7 cut(s)	58, 687, 754, 757, 891, 973, 1517
BccI	CCATC	6 cut(s)	221, 463, 770, 1009, 1139, 1541
BceAI	ACGGC	2 cut(s)	222, 255
BciT130I	CCWGG	2 cut(s)	1253, 1467
BciVI	GTATCC	2 cut(s)	960, 1100
BcoDI	GTCTC	2 cut(s)	932, 1172
BcuI	ACTAGT	3 cut(s)	23, 835, 941
BfaI	CTAG	6 cut(s)	24, 836, 887, 942, 1064, 1280
BfmI	CTRYAG	1 cut(s)	905
BfoI	RGCGCY	1 cut(s)	870
BfuAI	ACCTGC	1 cut(s)	1025
BfuI	GTATCC	2 cut(s)	960, 1100
BisI	GCNGC	8 cut(s)	72, 551, 676, 768, 771, 905, 987, 1506
BlsI	GCNGC	8 cut(s)	73, 552, 677, 769, 772, 906, 988, 1507
Bme1390I	CCNGG	2 cut(s)	1253, 1467
Bme18I	GGWCC	1 cut(s)	877
BmeT110I	CYCGRG	1 cut(s)	1003
BmgT120I	GGNCC	3 cut(s)	130, 877, 1255
BmiI	GGNNCC	2 cut(s)	697, 966
BmrFI	CCNGG	2 cut(s)	1253, 1467
BmsI	GCATC	4 cut(s)	514, 1021, 1060, 1315
BpiI	GAAGAC	2 cut(s)	369, 802
BpuEI	CTTGAG	2 cut(s)	38, 956
Bsa29I	ATCGAT	1 cut(s)	1323
BsaBI	GATNNNNATC	1 cut(s)	1170
BsaHI	GRCGYC	1 cut(s)	300
BsaJI	CCNNGG	5 cut(s)	87, 699, 811, 1004, 1251
BsaWI	WCCGGW	7 cut(s)	109, 116, 529, 961, 1103, 1261, 1450
BsaXI	ACNNNNNCTCC	4 cut(s)	489, 519, 1410, 1440
Bsc4I	CCNNNNNNNGG	7 cut(s)	88, 116, 227, 373, 1006, 1034, 1145
Bse118I	RCCGGY	3 cut(s)	185, 1217, 1261
Bse1I	ACTGG	2 cut(s)	428, 1349
Bse8I	GATNNNNATC	1 cut(s)	1170
BseAI	TCCGGA	2 cut(s)	529, 1450
BseBI	CCWGG	2 cut(s)	1253, 1467
BseCI	ATCGAT	1 cut(s)	1323
BseDI	CCNNGG	5 cut(s)	87, 699, 811, 1004, 1251
BseGI	GGATG	2 cut(s)	474, 529
BseJI	GATNNNNATC	1 cut(s)	1170
BseLI	CCNNNNNNNGG	7 cut(s)	88, 116, 227, 373, 1006, 1034, 1145
BseMII	CTCAG	2 cut(s)	53, 1002
BseNI	ACTGG	2 cut(s)	428, 1349
BseRI	GAGGAG	3 cut(s)	191, 1106, 1109
BseXI	GCAGC	7 cut(s)	58, 687, 754, 757, 891, 973, 1517
BseYI	CCCAGC	2 cut(s)	331, 609
BsgI	GTGCAG	1 cut(s)	1008
Bsh1236I	CGCG	2 cut(s)	407, 597
Bsh1285I	CGRYCG	1 cut(s)	256
BshFI	GGCC	7 cut(s)	131, 242, 514, 536, 804, 1049, 1256
BshNI	GGYRCC	1 cut(s)	695
BshTI	ACCGGT	1 cut(s)	1261
BshVI	ATCGAT	1 cut(s)	1323
BsiEI	CGRYCG	1 cut(s)	256
BsiHKAI	GWGCWC	3 cut(s)	104, 864, 1230
BsiHKCI	CYCGRG	1 cut(s)	1003
BslFI	GGGAC	2 cut(s)	313, 912
BslI	CCNNNNNNNGG	7 cut(s)	88, 116, 227, 373, 1006, 1034, 1145
BsmAI	GTCTC	2 cut(s)	932, 1172
BsmBI	CGTCTC	1 cut(s)	1172
BsmFI	GGGAC	2 cut(s)	313, 912
BsnI	GGCC	7 cut(s)	131, 242, 514, 536, 804, 1049, 1256
BsoBI	CYCGRG	1 cut(s)	1003
Bsp1286I	GDGCHC	3 cut(s)	104, 864, 1230
Bsp13I	TCCGGA	2 cut(s)	529, 1450
Bsp143I	GATC	3 cut(s)	815, 1510, 1551
Bsp19I	CCATGG	1 cut(s)	87
BspACI	CCGC	7 cut(s)	407, 465, 550, 553, 585, 653, 993
BspANI	GGCC	7 cut(s)	131, 242, 514, 536, 804, 1049, 1256
BspCNI	CTCAG	2 cut(s)	54, 1003
BspDI	ATCGAT	1 cut(s)	1323
BspEI	TCCGGA	2 cut(s)	529, 1450
BspFNI	CGCG	2 cut(s)	407, 597
BspHI	TCATGA	2 cut(s)	96, 1288
BspLI	GGNNCC	2 cut(s)	697, 966
BspMAI	CTGCAG	1 cut(s)	909
BspMI	ACCTGC	1 cut(s)	1025
BspPI	GGATC	3 cut(s)	823, 1505, 1559
BspT107I	GGYRCC	1 cut(s)	695
BsrFI	RCCGGY	3 cut(s)	185, 1217, 1261
BsrI	ACTGG	2 cut(s)	428, 1349
BssAI	RCCGGY	3 cut(s)	185, 1217, 1261
BssECI	CCNNGG	5 cut(s)	87, 699, 811, 1004, 1251
BssMI	GATC	3 cut(s)	815, 1510, 1551
BssNI	GRCGYC	1 cut(s)	300
BssSI	CACGAG	1 cut(s)	1515
BssT1I	CCWWGG	2 cut(s)	87, 811
Bst2BI	CACGAG	1 cut(s)	1515
Bst2UI	CCWGG	2 cut(s)	1253, 1467
Bst4CI	ACNGT	2 cut(s)	505, 1426
Bst6I	CTCTTC	2 cut(s)	201, 513
BstACI	GRCGYC	1 cut(s)	300
BstAPI	GCANNNNNTGC	1 cut(s)	1031
BstC8I	GCNNGC	3 cut(s)	298, 595, 997
BstDEI	CTNAG	4 cut(s)	62, 358, 980, 1011
BstDSI	CCRYGG	2 cut(s)	87, 699
BstF5I	GGATG	2 cut(s)	474, 529
BstFNI	CGCG	2 cut(s)	407, 597
BstH2I	RGCGCY	1 cut(s)	870
BstHHI	GCGC	1 cut(s)	869
BstKTI	GATC	3 cut(s)	818, 1513, 1554
BstMAI	GTCTC	2 cut(s)	932, 1172
BstMBI	GATC	3 cut(s)	815, 1510, 1551
BstMCI	CGRYCG	1 cut(s)	256
BstMWI	GCNNNNNNNGC	7 cut(s)	533, 859, 992, 1016, 1031, 1060, 1195
BstNI	CCWGG	2 cut(s)	1253, 1467
BstNSI	RCATGY	1 cut(s)	1535
BstSCI	CCNGG	2 cut(s)	1251, 1465
BstSFI	CTRYAG	1 cut(s)	905
BstUI	CGCG	2 cut(s)	407, 597
BstV1I	GCAGC	7 cut(s)	58, 687, 754, 757, 891, 973, 1517
BstV2I	GAAGAC	2 cut(s)	369, 802
Bsu15I	ATCGAT	1 cut(s)	1323
BsuI	GTATCC	2 cut(s)	960, 1100
BsuRI	GGCC	7 cut(s)	131, 242, 514, 536, 804, 1049, 1256
BsuTUI	ATCGAT	1 cut(s)	1323
BtgI	CCRYGG	2 cut(s)	87, 699
BtgZI	GCGATG	1 cut(s)	748
BtsCI	GGATG	2 cut(s)	474, 529
BtsI	GCAGTG	1 cut(s)	1122
BtsIMutI	CAGTG	1 cut(s)	1122
BveI	ACCTGC	1 cut(s)	1025
Cac8I	GCNNGC	3 cut(s)	298, 595, 997
CciI	TCATGA	2 cut(s)	96, 1288
CfoI	GCGC	1 cut(s)	869
Cfr10I	RCCGGY	3 cut(s)	185, 1217, 1261
Cfr13I	GGNCC	3 cut(s)	130, 877, 1255
ClaI	ATCGAT	1 cut(s)	1323
CseI	GACGC	4 cut(s)	289, 413, 586, 599
Csp6I	GTAC	2 cut(s)	31, 635
CspAI	ACCGGT	1 cut(s)	1261
CviAII	CATG	9 cut(s)	88, 97, 127, 616, 956, 1051, 1289, 1355, 1532
CviQI	GTAC	2 cut(s)	31, 635
DdeI	CTNAG	4 cut(s)	62, 358, 980, 1011
DpnI	GATC	3 cut(s)	817, 1512, 1553
DpnII	GATC	3 cut(s)	815, 1510, 1551
DrdI	GACNNNNNNGTC	1 cut(s)	596
DseDI	GACNNNNNNGTC	1 cut(s)	596
EaeI	YGGCCR	3 cut(s)	240, 512, 1047
Eam1104I	CTCTTC	2 cut(s)	201, 513
EarI	CTCTTC	2 cut(s)	201, 513
Eco130I	CCWWGG	2 cut(s)	87, 811
Eco47I	GGWCC	1 cut(s)	877
Eco88I	CYCGRG	1 cut(s)	1003
EcoO109I	RGGNCCY	2 cut(s)	877, 1255
EcoRII	CCWGG	2 cut(s)	1251, 1465
EcoT14I	CCWWGG	2 cut(s)	87, 811
ErhI	CCWWGG	2 cut(s)	87, 811
Esp3I	CGTCTC	1 cut(s)	1172
FaeI	CATG	9 cut(s)	91, 100, 130, 619, 959, 1054, 1292, 1358, 1535
FaqI	GGGAC	2 cut(s)	313, 912
FatI	CATG	9 cut(s)	87, 96, 126, 615, 955, 1050, 1288, 1354, 1531
FauI	CCCGC	1 cut(s)	458
FblI	GTMKAC	1 cut(s)	1567
Fnu4HI	GCNGC	8 cut(s)	72, 551, 676, 768, 771, 905, 987, 1506
FokI	GGATG	2 cut(s)	481, 536
Fsp4HI	GCNGC	8 cut(s)	72, 551, 676, 768, 771, 905, 987, 1506
FspBI	CTAG	6 cut(s)	24, 836, 887, 942, 1064, 1280
GlaI	GCGC	1 cut(s)	868
GluI	GCNGC	8 cut(s)	72, 551, 676, 768, 771, 905, 987, 1506
GsaI	CCCAGC	2 cut(s)	335, 613
HaeII	RGCGCY	1 cut(s)	870
HaeIII	GGCC	7 cut(s)	131, 242, 514, 536, 804, 1049, 1256
HgaI	GACGC	4 cut(s)	289, 413, 586, 599
HhaI	GCGC	1 cut(s)	869
Hin1I	GRCGYC	1 cut(s)	300
Hin1II	CATG	9 cut(s)	91, 100, 130, 619, 959, 1054, 1292, 1358, 1535
Hin6I	GCGC	1 cut(s)	867
HinP1I	GCGC	1 cut(s)	867
HincII	GTYRAC	3 cut(s)	403, 1339, 1568
HindII	GTYRAC	3 cut(s)	403, 1339, 1568
HinfI	GANTC	9 cut(s)	113, 151, 326, 751, 798, 883, 1171, 1247, 1447
HphI	GGTGA	6 cut(s)	99, 160, 751, 800, 1093, 1556
Hpy166II	GTNNAC	5 cut(s)	31, 403, 759, 1339, 1568
Hpy188I	TCNGA	2 cut(s)	176, 823
Hpy8I	GTNNAC	5 cut(s)	31, 403, 759, 1339, 1568
Hpy99I	CGWCG	3 cut(s)	305, 602, 1569
HpyAV	CCTTC	8 cut(s)	143, 154, 367, 371, 815, 889, 1292, 1372
HpyCH4III	ACNGT	2 cut(s)	505, 1426
HpyCH4IV	ACGT	1 cut(s)	1181
HpyCH4V	TGCA	8 cut(s)	560, 773, 853, 907, 1025, 1034, 1073, 1505
HpyF10VI	GCNNNNNNNGC	7 cut(s)	533, 859, 992, 1016, 1031, 1060, 1195
HpyF3I	CTNAG	4 cut(s)	62, 358, 980, 1011
HpySE526I	ACGT	1 cut(s)	1181
Hsp92I	GRCGYC	1 cut(s)	300
Hsp92II	CATG	9 cut(s)	91, 100, 130, 619, 959, 1054, 1292, 1358, 1535
HspAI	GCGC	1 cut(s)	867
Kpn2I	TCCGGA	2 cut(s)	529, 1450
Kzo9I	GATC	3 cut(s)	815, 1510, 1551
LmnI	GCTCC	4 cut(s)	532, 859, 964, 976
Lsp1109I	GCAGC	7 cut(s)	58, 687, 754, 757, 891, 973, 1517
LweI	GCATC	4 cut(s)	514, 1021, 1060, 1315
MaeI	CTAG	6 cut(s)	24, 836, 887, 942, 1064, 1280
MaeII	ACGT	1 cut(s)	1181
MaeIII	GTNAC	2 cut(s)	911, 1497
MalI	GATC	3 cut(s)	817, 1512, 1553
MboI	GATC	3 cut(s)	815, 1510, 1551
MboII	GAAGA	5 cut(s)	188, 374, 530, 807, 904
MfeI	CAATTG	2 cut(s)	165, 561
MhlI	GDGCHC	3 cut(s)	104, 864, 1230
MlsI	TGGCCA	1 cut(s)	1049
MluNI	TGGCCA	1 cut(s)	1049
MlyI	GAGTC	2 cut(s)	335, 792
Mox20I	TGGCCA	1 cut(s)	1049
MroI	TCCGGA	2 cut(s)	529, 1450
MscI	TGGCCA	1 cut(s)	1049
MseI	TTAA	5 cut(s)	456, 570, 1043, 1364, 1491
MslI	CAYNNNNRTG	1 cut(s)	1166
Msp20I	TGGCCA	1 cut(s)	1049
MspA1I	CMGCKG	2 cut(s)	587, 653
MspR9I	CCNGG	2 cut(s)	1253, 1467
MunI	CAATTG	2 cut(s)	165, 561
MvaI	CCWGG	2 cut(s)	1253, 1467
MvnI	CGCG	2 cut(s)	407, 597
MwoI	GCNNNNNNNGC	7 cut(s)	533, 859, 992, 1016, 1031, 1060, 1195
NcoI	CCATGG	1 cut(s)	87
NdeII	GATC	3 cut(s)	815, 1510, 1551
NlaIII	CATG	9 cut(s)	91, 100, 130, 619, 959, 1054, 1292, 1358, 1535
NlaIV	GGNNCC	2 cut(s)	697, 966
NmeAIII	GCCGAG	1 cut(s)	780
NmuCI	GTSAC	1 cut(s)	911
NspI	RCATGY	1 cut(s)	1535
PagI	TCATGA	2 cut(s)	96, 1288
PcsI	WCGNNNNNNNCGW	1 cut(s)	597
PfeI	GAWTC	7 cut(s)	113, 151, 751, 883, 1171, 1247, 1447
PflFI	GACNNNGTC	1 cut(s)	1334
PflMI	CCANNNNNTGG	1 cut(s)	88
PinAI	ACCGGT	1 cut(s)	1261
PkrI	GCNGC	8 cut(s)	73, 552, 677, 769, 772, 906, 988, 1507
PleI	GAGTC	2 cut(s)	334, 792
PpsI	GAGTC	2 cut(s)	334, 792
PpuMI	RGGWCCY	1 cut(s)	877
Psp5II	RGGWCCY	1 cut(s)	877
Psp6I	CCWGG	2 cut(s)	1251, 1465
PspFI	CCCAGC	2 cut(s)	331, 609
PspGI	CCWGG	2 cut(s)	1251, 1465
PspN4I	GGNNCC	2 cut(s)	697, 966
PspPI	GGNCC	3 cut(s)	130, 877, 1255
PspPPI	RGGWCCY	1 cut(s)	877
PstI	CTGCAG	1 cut(s)	909
PsyI	GACNNNGTC	1 cut(s)	1334
RsaI	GTAC	2 cut(s)	32, 636
RsaNI	GTAC	2 cut(s)	31, 635
RseI	CAYNNNNRTG	1 cut(s)	1166
SalI	GTCGAC	1 cut(s)	1566
SaqAI	TTAA	5 cut(s)	456, 570, 1043, 1364, 1491
SatI	GCNGC	8 cut(s)	72, 551, 676, 768, 771, 905, 987, 1506
Sau3AI	GATC	3 cut(s)	815, 1510, 1551
Sau96I	GGNCC	3 cut(s)	130, 877, 1255
SchI	GAGTC	2 cut(s)	335, 792
ScrFI	CCNGG	2 cut(s)	1253, 1467
SduI	GDGCHC	3 cut(s)	104, 864, 1230
SfaNI	GCATC	4 cut(s)	514, 1021, 1060, 1315
SfcI	CTRYAG	1 cut(s)	905
SinI	GGWCC	1 cut(s)	877
SmiMI	CAYNNNNRTG	1 cut(s)	1166
SmlI	CTYRAG	2 cut(s)	53, 971
SmoI	CTYRAG	2 cut(s)	53, 971
SpeI	ACTAGT	3 cut(s)	23, 835, 941
SsiI	CCGC	7 cut(s)	407, 465, 550, 553, 585, 653, 993
SspMI	CTAG	6 cut(s)	24, 836, 887, 942, 1064, 1280
StyD4I	CCNGG	2 cut(s)	1251, 1465
StyI	CCWWGG	2 cut(s)	87, 811
TaaI	ACNGT	2 cut(s)	505, 1426
TaiI	ACGT	1 cut(s)	1184
TaqI	TCGA	9 cut(s)	303, 315, 387, 600, 1236, 1308, 1323, 1485, 1567
TaqII	GACCGA	1 cut(s)	270
TatI	WGTACW	1 cut(s)	634
TauI	GCSGC	1 cut(s)	553
TfiI	GAWTC	7 cut(s)	113, 151, 751, 883, 1171, 1247, 1447
Tru1I	TTAA	5 cut(s)	456, 570, 1043, 1364, 1491
Tru9I	TTAA	5 cut(s)	456, 570, 1043, 1364, 1491
TscAI	CASTG	1 cut(s)	1129
TseFI	GTSAC	1 cut(s)	911
TseI	GCWGC	7 cut(s)	71, 675, 767, 770, 904, 986, 1505
Tsp45I	GTSAC	1 cut(s)	911
TspGWI	ACGGA	1 cut(s)	807
TspRI	CASTG	1 cut(s)	1129
Tth111I	GACNNNGTC	1 cut(s)	1334
Van91I	CCANNNNNTGG	1 cut(s)	88
VpaK11BI	GGWCC	1 cut(s)	877
XapI	RAATTY	2 cut(s)	603, 775
XbaI	TCTAGA	1 cut(s)	886
XceI	RCATGY	1 cut(s)	1535
XmiI	GTMKAC	1 cut(s)	1567
XspI	CTAG	6 cut(s)	24, 836, 887, 942, 1064, 1280

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.