Rorug02G0028300

O-methyltransferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0003495
Physical Location & Seq
Forward (+)
2253154 .. 2254092
939 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0028300.1

Sequence Viewer

Length: 489 bp
ATGGATAGCCGTGTGGCTTTGATGAGCTTCGTGATGGTGGCTTTGCTAATGAAGGGTGCAGCTGCTGGAATTCACAGTGTCAATTGGACTGTCCCCTCCAATAAGTCAGATTACACCACTTGGGCTAACACGACCACTTTCTATCTCTACGACCAACTGCAATTCAGGTGGAGTGGGACGCACAATGTGGCGAATGCAACCAAGGATGAATATGATAACTGCTTAAAGACTGCCAAGGTACTAAGTACCTCGTTGATCACTCTTATCGATCTTAATACTTCTGGTCCTCACTACTTCATATGCACCATAGACGATCACTGTGAAAGTGGCCAGAAGCTGGCCATCAACGTTACCAACGTTATCAGTCCCACGTTAACTACTCCACCGCCGCCACCAAGCTCAGCTTCAGCTCTGGCTGTTGGGACTTTATTTGCAATCGTGAGCTCCATCATAGTCACATCGTTCATTTATCGCTTCACACTATACTAA

Protein Analysis

162

Amino Acids

17.71

Weight (kDa)

6.38

Isoelectric Point (pI)

36.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 29 - 111 6.3e-19 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 337
AciI CCGC 2 cut(s) 386, 389
AclI AACGTT 2 cut(s) 348, 357
AcoI YGGCCR 2 cut(s) 328, 339
AcsI RAATTY 1 cut(s) 69
AcuI CTGAAG 1 cut(s) 390
AdeI CACNNNGTG 1 cut(s) 187
AfaI GTAC 2 cut(s) 240, 247
AfiI CCNNNNNNNGG 1 cut(s) 337
AjuI GAANNNNNNNTTGG 2 cut(s) 388, 420
AluBI AGCT 7 cut(s) 27, 62, 337, 399, 404, 410, 444
AluI AGCT 7 cut(s) 27, 62, 337, 399, 404, 410, 444
Alw21I GWGCWC 1 cut(s) 446
AlwNI CAGNNNCTG 2 cut(s) 65, 337
AoxI GGCC 2 cut(s) 328, 339
ApeKI GCWGC 2 cut(s) 59, 62
ApoI RAATTY 1 cut(s) 69
AspS9I GGNCC 1 cut(s) 284
AvaII GGWCC 1 cut(s) 284
BalI TGGCCA 2 cut(s) 330, 341
BanII GRGCYC 1 cut(s) 446
Bbv12I GWGCWC 1 cut(s) 446
BbvI GCAGC 2 cut(s) 49, 71
BccI CCATC 3 cut(s) 28, 350, 455
BclI TGATCA 1 cut(s) 255
BisI GCNGC 3 cut(s) 60, 63, 389
BlpI GCTNAGC 1 cut(s) 400
BlsI GCNGC 3 cut(s) 61, 64, 390
Bme18I GGWCC 1 cut(s) 284
BmgT120I GGNCC 1 cut(s) 284
Bpu1102I GCTNAGC 1 cut(s) 400
Bsa29I ATCGAT 1 cut(s) 267
BsaJI CCNNGG 2 cut(s) 201, 234
Bsc4I CCNNNNNNNGG 1 cut(s) 337
BseCI ATCGAT 1 cut(s) 267
BseDI CCNNGG 2 cut(s) 201, 234
BseGI GGATG 1 cut(s) 211
BseLI CCNNNNNNNGG 1 cut(s) 337
BseMII CTCAG 1 cut(s) 414
BseXI GCAGC 2 cut(s) 49, 71
BsgI GTGCAG 1 cut(s) 78
BshFI GGCC 2 cut(s) 330, 341
BshVI ATCGAT 1 cut(s) 267
BsiHKAI GWGCWC 1 cut(s) 446
BslFI GGGAC 4 cut(s) 77, 190, 351, 436
BslI CCNNNNNNNGG 1 cut(s) 337
BsmFI GGGAC 4 cut(s) 77, 190, 351, 436
BsmI GAATGC 1 cut(s) 199
BsnI GGCC 2 cut(s) 330, 341
Bsp1286I GDGCHC 1 cut(s) 446
Bsp143I GATC 3 cut(s) 255, 268, 313
Bsp1720I GCTNAGC 1 cut(s) 400
BspACI CCGC 2 cut(s) 386, 389
BspANI GGCC 2 cut(s) 330, 341
BspCNI CTCAG 1 cut(s) 413
BspDI ATCGAT 1 cut(s) 267
BssECI CCNNGG 2 cut(s) 201, 234
BssMI GATC 3 cut(s) 255, 268, 313
BssT1I CCWWGG 2 cut(s) 201, 234
Bst4CI ACNGT 3 cut(s) 77, 91, 320
BstC8I GCNNGC 1 cut(s) 339
BstDEI CTNAG 2 cut(s) 242, 400
BstF5I GGATG 1 cut(s) 211
BstKTI GATC 3 cut(s) 258, 271, 316
BstMBI GATC 3 cut(s) 255, 268, 313
BstV1I GCAGC 2 cut(s) 49, 71
Bsu15I ATCGAT 1 cut(s) 267
BsuRI GGCC 2 cut(s) 330, 341
BsuTUI ATCGAT 1 cut(s) 267
BtsCI GGATG 1 cut(s) 211
BtsIMutI CAGTG 2 cut(s) 82, 316
Cac8I GCNNGC 1 cut(s) 339
CaiI CAGNNNCTG 2 cut(s) 65, 337
Cfr13I GGNCC 1 cut(s) 284
ClaI ATCGAT 1 cut(s) 267
CseI GACGC 1 cut(s) 187
Csp6I GTAC 2 cut(s) 239, 246
CspCI CAANNNNNGTGG 2 cut(s) 149, 184
CviQI GTAC 2 cut(s) 239, 246
DdeI CTNAG 2 cut(s) 242, 400
DpnI GATC 3 cut(s) 257, 270, 315
DpnII GATC 3 cut(s) 255, 268, 313
DraIII CACNNNGTG 1 cut(s) 187
EaeI YGGCCR 2 cut(s) 328, 339
Ecl136II GAGCTC 1 cut(s) 444
Eco130I CCWWGG 2 cut(s) 201, 234
Eco24I GRGCYC 1 cut(s) 446
Eco47I GGWCC 1 cut(s) 284
Eco53kI GAGCTC 1 cut(s) 444
Eco57I CTGAAG 1 cut(s) 390
EcoICRI GAGCTC 1 cut(s) 444
EcoRI GAATTC 1 cut(s) 69
EcoT14I CCWWGG 2 cut(s) 201, 234
EcoT38I GRGCYC 1 cut(s) 446
ErhI CCWWGG 2 cut(s) 201, 234
FaiI YATR 6 cut(s) 213, 299, 301, 308, 452, 484
FalI AAGNNNNNCTT 2 cut(s) 388, 420
FaqI GGGAC 4 cut(s) 77, 190, 351, 436
FauNDI CATATG 1 cut(s) 299
FbaI TGATCA 1 cut(s) 255
Fnu4HI GCNGC 3 cut(s) 60, 63, 389
FokI GGATG 1 cut(s) 218
FriOI GRGCYC 1 cut(s) 446
Fsp4HI GCNGC 3 cut(s) 60, 63, 389
GluI GCNGC 3 cut(s) 60, 63, 389
HaeIII GGCC 2 cut(s) 330, 341
HgaI GACGC 1 cut(s) 187
HincII GTYRAC 1 cut(s) 375
HindII GTYRAC 1 cut(s) 375
HpaI GTTAAC 1 cut(s) 375
Hpy166II GTNNAC 1 cut(s) 375
Hpy188I TCNGA 1 cut(s) 109
Hpy188III TCNNGA 2 cut(s) 31, 439
Hpy8I GTNNAC 1 cut(s) 375
HpyAV CCTTC 1 cut(s) 46
HpyCH4III ACNGT 3 cut(s) 77, 91, 320
HpyCH4IV ACGT 3 cut(s) 348, 357, 371
HpyCH4V TGCA 5 cut(s) 59, 160, 197, 303, 434
HpyF3I CTNAG 2 cut(s) 242, 400
HpySE526I ACGT 3 cut(s) 348, 357, 371
Ksp22I TGATCA 1 cut(s) 255
KspAI GTTAAC 1 cut(s) 375
Kzo9I GATC 3 cut(s) 255, 268, 313
LmnI GCTCC 1 cut(s) 449
LpnPI CCDG 6 cut(s) 51, 151, 267, 323, 344, 398
Lsp1109I GCAGC 2 cut(s) 49, 71
MaeII ACGT 3 cut(s) 348, 357, 371
MaeIII GTNAC 2 cut(s) 349, 454
MalI GATC 3 cut(s) 257, 270, 315
MboI GATC 3 cut(s) 255, 268, 313
MfeI CAATTG 1 cut(s) 82
MhlI GDGCHC 1 cut(s) 446
MlsI TGGCCA 2 cut(s) 330, 341
MluCI AATT 3 cut(s) 69, 82, 161
MluNI TGGCCA 2 cut(s) 330, 341
MnlI CCTC 3 cut(s) 106, 259, 297
Mox20I TGGCCA 2 cut(s) 330, 341
MscI TGGCCA 2 cut(s) 330, 341
MseI TTAA 3 cut(s) 224, 273, 374
Msp20I TGGCCA 2 cut(s) 330, 341
MspA1I CMGCKG 1 cut(s) 62
MunI CAATTG 1 cut(s) 82
Mva1269I GAATGC 1 cut(s) 199
NdeI CATATG 1 cut(s) 299
NdeII GATC 3 cut(s) 255, 268, 313
NmuCI GTSAC 1 cut(s) 454
PcsI WCGNNNNNNNCGW 1 cut(s) 354
PctI GAATGC 1 cut(s) 199
PflMI CCANNNNNTGG 1 cut(s) 337
PkrI GCNGC 3 cut(s) 61, 64, 390
Psp124BI GAGCTC 1 cut(s) 446
Psp1406I AACGTT 2 cut(s) 348, 357
PspPI GGNCC 1 cut(s) 284
PstNI CAGNNNCTG 2 cut(s) 65, 337
PvuII CAGCTG 1 cut(s) 62
RsaI GTAC 2 cut(s) 240, 247
RsaNI GTAC 2 cut(s) 239, 246
SacI GAGCTC 1 cut(s) 446
SaqAI TTAA 3 cut(s) 224, 273, 374
SatI GCNGC 3 cut(s) 60, 63, 389
Sau3AI GATC 3 cut(s) 255, 268, 313
Sau96I GGNCC 1 cut(s) 284
SduI GDGCHC 1 cut(s) 446
SinI GGWCC 1 cut(s) 284
Sse9I AATT 3 cut(s) 69, 82, 161
SsiI CCGC 2 cut(s) 386, 389
SstI GAGCTC 1 cut(s) 446
StyI CCWWGG 2 cut(s) 201, 234
TaaI ACNGT 3 cut(s) 77, 91, 320
TaiI ACGT 3 cut(s) 351, 360, 374
TaqI TCGA 1 cut(s) 267
TasI AATT 3 cut(s) 69, 82, 161
TauI GCSGC 1 cut(s) 391
Tru1I TTAA 3 cut(s) 224, 273, 374
Tru9I TTAA 3 cut(s) 224, 273, 374
TscAI CASTG 2 cut(s) 82, 323
TseFI GTSAC 1 cut(s) 454
TseI GCWGC 2 cut(s) 59, 62
Tsp45I GTSAC 1 cut(s) 454
TspDTI ATGAA 4 cut(s) 65, 222, 286, 454
TspRI CASTG 2 cut(s) 82, 323
Van91I CCANNNNNTGG 1 cut(s) 337
VpaK11BI GGWCC 1 cut(s) 284
XapI RAATTY 1 cut(s) 69
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.