Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0003495

Physical Location & Seq

Genomic Coordinates Reverse (-)

5699276 .. 5702660

Gene Structure 3385 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000016329

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1509 bp

ATGGAGCAGAACAAGAAAAAGGTATCGAAGGGACTATTGCAGAGTGATGACTTGTATCAGTATATCTTGGAGACTAGCGTGTATCCGCGTGAACCGGAGCCTCTCAAGGAGCTGAGAGAGGCCACTGCCAGCCACCCCGGGGCCGGGATGGGTACTGCACCAGATGCAGGTCAGTTAATGACCATGCTATTGAAGTTAGTAGATGCAAAGAAGACGATTGAAGTTGGAGTTTTTACCGGATACTCTCTTCTCCTCACTGCTCTTACAATTCCTGAGGATGGCAAGATTGTCGCCATAGATGTAGATCGTGAGCCGTATGATCAAATAGGATTTCCAATCATAAAGAAAGCTGATGTTGAGCACAAAATTGACCTCATTGAATCTGAGGCCCTACCCGTTCTTGATAAGCTATCGGAACAAGCAAGTATCACGCATTTTCTTTTTAAACTCCAACTGCATGAAAATGAAGGAAGTTTCGACTTTGCTTTCGTTGATGCAGACAAGGTTAACTATTGCAATTACCACGAGAGACTAATGAAGCTGCTGAAGGTGGGGGGTATAGTTGTGTATGATAACACTCTTTGGGGAGGGACAGTTGTAATGCCTGTTGAGTCGACTCCGGAACAGATGAAACCAAGCAGACAGTCTATTATGGAGCTCAATAAGTTGCTCGCTGCTGATACTCGTGTCCAAATTTCGCATGCTGCTTTGGGTGATGGGATCACAGTTTTCTTATTTTCTCTGTTCATTGAATCATACAGATTATCAGAACTTTATTGTGAATATTGTCCGAGAGAAATGGAGCACAACACGAAAAAGGTATCAAAGGGACTATTGCAGAATGATGAATTGTATCAGTATGTCTTGGAGACTAGTGTGTATCCGCGTGAACCGGAGCCTCTCAAGGAGCTTAGGGATGCCACTGCCAGCCACCCCAGGGCTGAGATGGCTACTGCACCAGATGCAGGCCAGTTGATGACCATGCTGTTGGGGCTAGTAGATGCAAAGAAGACGATTGAAGTTGGAGTTTTTACTGGATACTCTCTTCTCCTCACTGCTCTTGCAATTCCTGAGGATGGCAAGATTTTAGCCATAGATGTAAACCGTGAGCCATATGATCAAATAGGATTTCCAATCATAAAGAAAGCTGGTGTTGAGCACAAAATTGACCTCATTGAATCTGAGGCGCTGCCAGTTCTTGATAAGCTATTGGAACAACATGAAAATGAAGGAAGTTTTGACTTTGCTTTTGTCGATGCGGACAAGGTTAACTATTGGAATTACCATGAGAAACTAATGAAGCTGCTGAAGGTGGGAGGTATAGCTGTGTATGATAACACTCTTTGGGGAGGGTCAGTTGTAATGCCTATTGAGTCGACTCCAGAACACATGAAACCAGGCAGACAGTATACTATAGAGCTCAACAAGTTACTTGCTGCTGATACTCGTGTCCAAATTTCGCATGCTTCTTTGGGTGATGGGATCACAATTTGTAGGCGACTATACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0008150 GO:0008152 GO:0008168 GO:0008171 GO:0008757 GO:0016740 GO:0016741 GO:0032259 GO:0042409 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00360 ko00940 ko00941 ko00945 ko01100 ko01110 map00360 map00940 map00941 map00945 map01100 map01110

Pfam Domains

Protein Families

Methyltransf_3

Protein Analysis

503

Amino Acids

56.23

Weight (kDa)

5.06

Isoelectric Point (pI)

30.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Methyltransf_3	PF01596	24 - 243	2e-69	O-methyltransferase
Methyltransf_24	PF13578	73 - 192	4.5e-07	Methyltransferase domain
Methyltransf_3	PF01596	290 - 501	2.1e-75	O-methyltransferase
Methyltransf_24	PF13578	339 - 446	6.5e-08	Methyltransferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003495)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G26220
fragaria_vesca	FvH4_1g06840 FvH4_1g06841 FvH4_1g06841
malus_domestica	MD02G1073100.v1.1 MD02G1073400.v1.1
prunus_persica	Prupe.7G214400_v2.0.a1 Prupe.7G214400_v2.0.a1 Prupe.7G214400_v2.0.a1
pyrus_communis	pycom02g05730 pycom02g05760 pycom02g05770
rosa_chinensis	RchiOBHm_Chr2g0092621 RchiOBHm_Chr2g0092631 RchiOBHm_Chr2g0093901 RchiOBHm_Chr2g0093911
rosa_laevigata	RLG00000016207 RLG00000016329
rosa_multiflora	Rmu_co8197646.1_g000001 Rmu_co8235837.1_g000001 Rmu_co8321945.1_g000001 Rmu_sc0003027.1_g000003
rosa_roxburghii	Rroxscaffold_2G00147620 Rroxscaffold_2G00147650 Rroxscaffold_2G00148920
rosa_rugosa	Rorug02G0028200 Rorug02G0028300
rosa_samantha	Rh2AG074000 Rh2AG074100 Rh2BG075100 Rh2BG075200 Rh2CG076700 Rh2CG076800
rosa_wichuraiana	Rw2G005890 Rw2G005900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	158
AccI	GTMKAC	3 cut(s)	614, 1376, 1409
AccII	CGCG	2 cut(s)	88, 886
AccIII	TCCGGA	1 cut(s)	619
AciI	CCGC	3 cut(s)	86, 884, 1259
AclWI	GGATC	2 cut(s)	728, 1490
AcsI	RAATTY	2 cut(s)	693, 1455
AcuI	CTGAAG	2 cut(s)	566, 1328
AfaI	GTAC	1 cut(s)	154
AfiI	CCNNNNNNNGG	8 cut(s)	139, 143, 144, 167, 278, 937, 965, 1076
AgsI	TTSAA	6 cut(s)	193, 221, 380, 752, 1019, 1178
AhlI	ACTAGT	1 cut(s)	872
AjnI	CCWGG	2 cut(s)	935, 1396
Alw21I	GWGCWC	5 cut(s)	363, 660, 807, 1161, 1422
Alw26I	GTCTC	3 cut(s)	65, 523, 863
AlwI	GGATC	2 cut(s)	728, 1490
Ama87I	CYCGRG	1 cut(s)	137
Aor13HI	TCCGGA	1 cut(s)	619
AoxI	GGCC	4 cut(s)	120, 141, 387, 967
ApeKI	GCWGC	6 cut(s)	541, 674, 704, 1189, 1303, 1436
ApoI	RAATTY	2 cut(s)	693, 1455
ArsI	GACNNNNNNTTYG	4 cut(s)	470, 502, 1232, 1264
AspLEI	GCGC	1 cut(s)	1189
AspS9I	GGNCC	2 cut(s)	141, 388
AsuC2I	CCSGG	3 cut(s)	138, 139, 145
AsuHPI	GGTGA	2 cut(s)	725, 1487
AvaI	CYCGRG	1 cut(s)	137
AxyI	CCTNAGG	2 cut(s)	273, 1071
BaeI	ACNNNNGTAYC	4 cut(s)	672, 705, 1434, 1467
BanII	GRGCYC	2 cut(s)	660, 1422
BauI	CACGAG	3 cut(s)	524, 684, 1446
BbsI	GAAGAC	2 cut(s)	218, 1016
Bbv12I	GWGCWC	5 cut(s)	363, 660, 807, 1161, 1422
BbvI	GCAGC	6 cut(s)	528, 661, 691, 1176, 1290, 1423
BccI	CCATC	6 cut(s)	142, 272, 710, 940, 1070, 1472
BceAI	ACGGC	1 cut(s)	298
BcgI	CGANNNNNNTGC	2 cut(s)	271, 305
BciT130I	CCWGG	2 cut(s)	937, 1398
BciVI	GTATCC	4 cut(s)	93, 233, 891, 1031
BclI	TGATCA	2 cut(s)	319, 1117
BcnI	CCSGG	3 cut(s)	138, 139, 145
BcoDI	GTCTC	3 cut(s)	65, 523, 863
BcuI	ACTAGT	1 cut(s)	872
BfaI	CTAG	3 cut(s)	75, 873, 995
BfmI	CTRYAG	1 cut(s)	1413
BfoI	RGCGCY	1 cut(s)	1190
BfuAI	ACCTGC	1 cut(s)	158
BfuI	GTATCC	4 cut(s)	93, 233, 891, 1031
BisI	GCNGC	6 cut(s)	542, 675, 705, 1190, 1304, 1437
BlsI	GCNGC	6 cut(s)	543, 676, 706, 1191, 1305, 1438
Bme1390I	CCNGG	5 cut(s)	138, 139, 145, 937, 1398
BmeT110I	CYCGRG	1 cut(s)	137
BmgT120I	GGNCC	2 cut(s)	141, 388
BmiI	GGNNCC	3 cut(s)	99, 142, 897
BmrFI	CCNGG	5 cut(s)	138, 139, 145, 937, 1398
BmsI	GCATC	7 cut(s)	154, 193, 484, 907, 952, 991, 1246
BpiI	GAAGAC	2 cut(s)	218, 1016
BpmI	CTGGAG	1 cut(s)	1365
Bpu10I	CCTNAGC	1 cut(s)	911
BpuEI	CTTGAG	2 cut(s)	89, 887
BpuMI	CCSGG	3 cut(s)	138, 139, 145
BsaBI	GATNNNNATC	1 cut(s)	303
BsaJI	CCNNGG	5 cut(s)	136, 137, 138, 935, 936
BsaWI	WCCGGW	4 cut(s)	94, 236, 619, 892
BsaXI	ACNNNNNCTCC	4 cut(s)	579, 609, 1341, 1371
Bsc4I	CCNNNNNNNGG	8 cut(s)	139, 143, 144, 167, 278, 937, 965, 1076
Bse1I	ACTGG	3 cut(s)	970, 1039, 1193
Bse21I	CCTNAGG	2 cut(s)	273, 1071
Bse8I	GATNNNNATC	1 cut(s)	303
BseAI	TCCGGA	1 cut(s)	619
BseBI	CCWGG	2 cut(s)	937, 1398
BseDI	CCNNGG	5 cut(s)	136, 137, 138, 935, 936
BseGI	GGATG	4 cut(s)	153, 283, 922, 1081
BseJI	GATNNNNATC	1 cut(s)	303
BseLI	CCNNNNNNNGG	8 cut(s)	139, 143, 144, 167, 278, 937, 965, 1076
BseMII	CTCAG	6 cut(s)	104, 264, 375, 933, 1062, 1173
BseNI	ACTGG	3 cut(s)	970, 1039, 1193
BseRI	GAGGAG	2 cut(s)	242, 1040
BseXI	GCAGC	6 cut(s)	528, 661, 691, 1176, 1290, 1423
BsgI	GTGCAG	2 cut(s)	141, 939
Bsh1236I	CGCG	2 cut(s)	88, 886
BshFI	GGCC	4 cut(s)	122, 143, 389, 969
BsiHKAI	GWGCWC	5 cut(s)	363, 660, 807, 1161, 1422
BsiHKCI	CYCGRG	1 cut(s)	137
BsiSI	CCGG	6 cut(s)	95, 138, 144, 237, 620, 893
BslFI	GGGAC	3 cut(s)	45, 604, 843
BslI	CCNNNNNNNGG	8 cut(s)	139, 143, 144, 167, 278, 937, 965, 1076
BsmAI	GTCTC	3 cut(s)	65, 523, 863
BsmFI	GGGAC	3 cut(s)	45, 604, 843
BsnI	GGCC	4 cut(s)	122, 143, 389, 969
BsoBI	CYCGRG	1 cut(s)	137
Bsp1286I	GDGCHC	5 cut(s)	363, 660, 807, 1161, 1422
Bsp13I	TCCGGA	1 cut(s)	619
Bsp143I	GATC	5 cut(s)	304, 319, 720, 1117, 1482
BspACI	CCGC	3 cut(s)	86, 884, 1259
BspANI	GGCC	4 cut(s)	122, 143, 389, 969
BspCNI	CTCAG	6 cut(s)	105, 265, 376, 934, 1063, 1174
BspEI	TCCGGA	1 cut(s)	619
BspFNI	CGCG	2 cut(s)	88, 886
BspLI	GGNNCC	3 cut(s)	99, 142, 897
BspMI	ACCTGC	1 cut(s)	158
BspPI	GGATC	2 cut(s)	728, 1490
BsrI	ACTGG	3 cut(s)	970, 1039, 1193
BssECI	CCNNGG	5 cut(s)	136, 137, 138, 935, 936
BssMI	GATC	5 cut(s)	304, 319, 720, 1117, 1482
BssNAI	GTATAC	1 cut(s)	1410
BssSI	CACGAG	3 cut(s)	524, 684, 1446
Bst1107I	GTATAC	1 cut(s)	1410
Bst2BI	CACGAG	3 cut(s)	524, 684, 1446
Bst2UI	CCWGG	2 cut(s)	937, 1398
Bst4CI	ACNGT	5 cut(s)	595, 645, 727, 1106, 1407
Bst6I	CTCTTC	2 cut(s)	252, 1050
BstAPI	GCANNNNNTGC	2 cut(s)	164, 962
BstC8I	GCNNGC	6 cut(s)	130, 672, 702, 928, 967, 1464
BstDEI	CTNAG	7 cut(s)	113, 273, 384, 911, 942, 1071, 1182
BstF5I	GGATG	4 cut(s)	153, 283, 922, 1081
BstFNI	CGCG	2 cut(s)	88, 886
BstH2I	RGCGCY	1 cut(s)	1190
BstHHI	GCGC	1 cut(s)	1189
BstKTI	GATC	5 cut(s)	307, 322, 723, 1120, 1485
BstMAI	GTCTC	3 cut(s)	65, 523, 863
BstMBI	GATC	5 cut(s)	304, 319, 720, 1117, 1482
BstMWI	GCNNNNNNNGC	4 cut(s)	164, 947, 962, 991
BstNI	CCWGG	2 cut(s)	937, 1398
BstNSI	RCATGY	2 cut(s)	704, 1466
BstSCI	CCNGG	5 cut(s)	136, 137, 143, 935, 1396
BstSFI	CTRYAG	1 cut(s)	1413
BstUI	CGCG	2 cut(s)	88, 886
BstV1I	GCAGC	6 cut(s)	528, 661, 691, 1176, 1290, 1423
BstV2I	GAAGAC	2 cut(s)	218, 1016
BstXI	CCANNNNNNTGG	1 cut(s)	988
BstZ17I	GTATAC	1 cut(s)	1410
Bsu36I	CCTNAGG	2 cut(s)	273, 1071
BsuI	GTATCC	4 cut(s)	93, 233, 891, 1031
BsuRI	GGCC	4 cut(s)	122, 143, 389, 969
BtsCI	GGATG	4 cut(s)	153, 283, 922, 1081
BtsI	GCAGTG	4 cut(s)	123, 255, 921, 1053
BtsIMutI	CAGTG	4 cut(s)	123, 255, 921, 1053
BveI	ACCTGC	1 cut(s)	158
Cac8I	GCNNGC	6 cut(s)	130, 672, 702, 928, 967, 1464
CfoI	GCGC	1 cut(s)	1189
Cfr13I	GGNCC	2 cut(s)	141, 388
Cfr9I	CCCGGG	1 cut(s)	137
Csp6I	GTAC	1 cut(s)	153
CviAII	CATG	8 cut(s)	184, 458, 701, 982, 1220, 1286, 1390, 1463
CviQI	GTAC	1 cut(s)	153
DdeI	CTNAG	7 cut(s)	113, 273, 384, 911, 942, 1071, 1182
DpnI	GATC	5 cut(s)	306, 321, 722, 1119, 1484
DpnII	GATC	5 cut(s)	304, 319, 720, 1117, 1482
DraI	TTTAAA	1 cut(s)	445
Eam1104I	CTCTTC	2 cut(s)	252, 1050
EarI	CTCTTC	2 cut(s)	252, 1050
Ecl136II	GAGCTC	2 cut(s)	658, 1420
Eco24I	GRGCYC	2 cut(s)	660, 1422
Eco53kI	GAGCTC	2 cut(s)	658, 1420
Eco57I	CTGAAG	2 cut(s)	566, 1328
Eco81I	CCTNAGG	2 cut(s)	273, 1071
Eco88I	CYCGRG	1 cut(s)	137
EcoICRI	GAGCTC	2 cut(s)	658, 1420
EcoO109I	RGGNCCY	1 cut(s)	388
EcoRII	CCWGG	2 cut(s)	935, 1396
EcoT38I	GRGCYC	2 cut(s)	660, 1422
FaeI	CATG	8 cut(s)	187, 461, 704, 985, 1223, 1289, 1393, 1466
FaqI	GGGAC	3 cut(s)	45, 604, 843
FatI	CATG	8 cut(s)	183, 457, 700, 981, 1219, 1285, 1389, 1462
FauNDI	CATATG	1 cut(s)	1114
FbaI	TGATCA	2 cut(s)	319, 1117
FblI	GTMKAC	3 cut(s)	614, 1376, 1409
Fnu4HI	GCNGC	6 cut(s)	542, 675, 705, 1190, 1304, 1437
FokI	GGATG	4 cut(s)	160, 290, 929, 1088
FriOI	GRGCYC	2 cut(s)	660, 1422
Fsp4HI	GCNGC	6 cut(s)	542, 675, 705, 1190, 1304, 1437
FspBI	CTAG	3 cut(s)	75, 873, 995
GlaI	GCGC	1 cut(s)	1188
GluI	GCNGC	6 cut(s)	542, 675, 705, 1190, 1304, 1437
GsuI	CTGGAG	1 cut(s)	1365
HaeII	RGCGCY	1 cut(s)	1190
HaeIII	GGCC	4 cut(s)	122, 143, 389, 969
HapII	CCGG	6 cut(s)	95, 138, 144, 237, 620, 893
HhaI	GCGC	1 cut(s)	1189
Hin1II	CATG	8 cut(s)	187, 461, 704, 985, 1223, 1289, 1393, 1466
Hin6I	GCGC	1 cut(s)	1187
HinP1I	GCGC	1 cut(s)	1187
HincII	GTYRAC	4 cut(s)	508, 615, 1270, 1377
HindII	GTYRAC	4 cut(s)	508, 615, 1270, 1377
HinfI	GANTC	7 cut(s)	380, 611, 616, 752, 1178, 1373, 1378
HpaI	GTTAAC	2 cut(s)	508, 1270
HpaII	CCGG	6 cut(s)	95, 138, 144, 237, 620, 893
HphI	GGTGA	2 cut(s)	725, 1487
Hpy166II	GTNNAC	8 cut(s)	92, 508, 615, 890, 1102, 1270, 1377, 1410
Hpy188I	TCNGA	5 cut(s)	385, 415, 769, 792, 1183
Hpy188III	TCNNGA	7 cut(s)	272, 308, 401, 620, 1070, 1199, 1382
Hpy8I	GTNNAC	8 cut(s)	92, 508, 615, 890, 1102, 1270, 1377, 1410
HpyAV	CCTTC	5 cut(s)	22, 461, 541, 1223, 1303
HpyCH4III	ACNGT	5 cut(s)	595, 645, 727, 1106, 1407
HpyF10VI	GCNNNNNNNGC	4 cut(s)	164, 947, 962, 991
HpyF3I	CTNAG	7 cut(s)	113, 273, 384, 911, 942, 1071, 1182
Hsp92II	CATG	8 cut(s)	187, 461, 704, 985, 1223, 1289, 1393, 1466
HspAI	GCGC	1 cut(s)	1187
Kpn2I	TCCGGA	1 cut(s)	619
Ksp22I	TGATCA	2 cut(s)	319, 1117
KspAI	GTTAAC	2 cut(s)	508, 1270
Kzo9I	GATC	5 cut(s)	304, 319, 720, 1117, 1482
LmnI	GCTCC	7 cut(s)	4, 97, 109, 655, 802, 895, 907
Lsp1109I	GCAGC	6 cut(s)	528, 661, 691, 1176, 1290, 1423
LweI	GCATC	7 cut(s)	154, 193, 484, 907, 952, 991, 1246
MaeI	CTAG	3 cut(s)	75, 873, 995
MaeIII	GTNAC	1 cut(s)	1428
MalI	GATC	5 cut(s)	306, 321, 722, 1119, 1484
MboI	GATC	5 cut(s)	304, 319, 720, 1117, 1482
MboII	GAAGA	4 cut(s)	223, 239, 1021, 1037
MhlI	GDGCHC	5 cut(s)	363, 660, 807, 1161, 1422
MlyI	GAGTC	4 cut(s)	610, 620, 1372, 1382
MmeI	TCCRAC	3 cut(s)	205, 475, 1003
MroI	TCCGGA	1 cut(s)	619
MseI	TTAA	4 cut(s)	176, 444, 507, 1269
MslI	CAYNNNNRTG	2 cut(s)	462, 1224
MspI	CCGG	6 cut(s)	95, 138, 144, 237, 620, 893
MspR9I	CCNGG	5 cut(s)	138, 139, 145, 937, 1398
MvaI	CCWGG	2 cut(s)	937, 1398
MvnI	CGCG	2 cut(s)	88, 886
MwoI	GCNNNNNNNGC	4 cut(s)	164, 947, 962, 991
NciI	CCSGG	3 cut(s)	138, 139, 145
NdeI	CATATG	1 cut(s)	1114
NdeII	GATC	5 cut(s)	304, 319, 720, 1117, 1482
NlaIII	CATG	8 cut(s)	187, 461, 704, 985, 1223, 1289, 1393, 1466
NlaIV	GGNNCC	3 cut(s)	99, 142, 897
NspI	RCATGY	2 cut(s)	704, 1466
PaeI	GCATGC	2 cut(s)	704, 1466
PasI	CCCWGGG	1 cut(s)	936
PfeI	GAWTC	3 cut(s)	380, 752, 1178
PkrI	GCNGC	6 cut(s)	543, 676, 706, 1191, 1305, 1438
PleI	GAGTC	4 cut(s)	610, 619, 1372, 1381
PpsI	GAGTC	4 cut(s)	610, 619, 1372, 1381
Psp124BI	GAGCTC	2 cut(s)	660, 1422
Psp6I	CCWGG	2 cut(s)	935, 1396
PspGI	CCWGG	2 cut(s)	935, 1396
PspN4I	GGNNCC	3 cut(s)	99, 142, 897
PspPI	GGNCC	2 cut(s)	141, 388
RsaI	GTAC	1 cut(s)	154
RsaNI	GTAC	1 cut(s)	153
RseI	CAYNNNNRTG	2 cut(s)	462, 1224
SacI	GAGCTC	2 cut(s)	660, 1422
SalI	GTCGAC	2 cut(s)	613, 1375
SaqAI	TTAA	4 cut(s)	176, 444, 507, 1269
SatI	GCNGC	6 cut(s)	542, 675, 705, 1190, 1304, 1437
Sau3AI	GATC	5 cut(s)	304, 319, 720, 1117, 1482
Sau96I	GGNCC	2 cut(s)	141, 388
SchI	GAGTC	4 cut(s)	610, 620, 1372, 1382
ScrFI	CCNGG	5 cut(s)	138, 139, 145, 937, 1398
SduI	GDGCHC	5 cut(s)	363, 660, 807, 1161, 1422
SfaNI	GCATC	7 cut(s)	154, 193, 484, 907, 952, 991, 1246
SfcI	CTRYAG	1 cut(s)	1413
SmaI	CCCGGG	1 cut(s)	139
SmiMI	CAYNNNNRTG	2 cut(s)	462, 1224
SmlI	CTYRAG	2 cut(s)	104, 902
SmoI	CTYRAG	2 cut(s)	104, 902
SpeI	ACTAGT	1 cut(s)	872
SphI	GCATGC	2 cut(s)	704, 1466
SsiI	CCGC	3 cut(s)	86, 884, 1259
SspI	AATATT	1 cut(s)	785
SspMI	CTAG	3 cut(s)	75, 873, 995
SstI	GAGCTC	2 cut(s)	660, 1422
StyD4I	CCNGG	5 cut(s)	136, 137, 143, 935, 1396
TaaI	ACNGT	5 cut(s)	595, 645, 727, 1106, 1407
TaqI	TCGA	5 cut(s)	26, 477, 614, 1254, 1376
TfiI	GAWTC	3 cut(s)	380, 752, 1178
Tru1I	TTAA	4 cut(s)	176, 444, 507, 1269
Tru9I	TTAA	4 cut(s)	176, 444, 507, 1269
TscAI	CASTG	4 cut(s)	130, 262, 928, 1060
TseI	GCWGC	6 cut(s)	541, 674, 704, 1189, 1303, 1436
TspMI	CCCGGG	1 cut(s)	137
TspRI	CASTG	4 cut(s)	130, 262, 928, 1060
XapI	RAATTY	2 cut(s)	693, 1455
XceI	RCATGY	2 cut(s)	704, 1466
XcmI	CCANNNNNNNNNTGG	1 cut(s)	943
XmaI	CCCGGG	1 cut(s)	137
XmiI	GTMKAC	3 cut(s)	614, 1376, 1409
XspI	CTAG	3 cut(s)	75, 873, 995

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.