Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0003495

Physical Location & Seq

Genomic Coordinates Forward (+)

86540073 .. 86548883

Gene Structure 8811 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00148920.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2325 bp

ATGGAATCAAGAGGGATTAAGATCTCAAAGGGCGTGTTGCAGAGTGATGAATTATCTCAATACATCTTGGAGACTAGTGTGTATCCACGTGAACCAGCGCCTCTCAAGGAGCTAAGGGATGCCACTGCCAGCCACCCTCGGGCTGGATTGGCTACTGCGCCAGATGCAGCTCAGTTACTAGCCGTGCTGTTGAAGCTCATAGATGCAAAGAAGACAATTGAAATTGGTGTTTTCACGGGATATTCTCTTCTCCTCACTGCGCTTACAATTCCTGAGGATGGCAAGATTGTAGCCATAGATATAAATCGTGAGACATATGATCAAATAGGCTTGCCGATCATAAAGAAAGCTGGTGTTGAGCACAAAATTGACTTCATTGAATCTGAGGCTGTACCAGTTCTTGATAAGCTATTGGAACATCCTGAAAATGAAGGCAGTTCTGACTTTGCCTATGTGGATGCCGACAAGGTTAACAATTGGAATTACCACGAGAGACTACTGAAGCTGTTGAAGGTGGGTGGCTTAGTTGTCTATGACAACACACTCTGCAGAGGATCAGTTGCGTTGCCTGATGAGTTAGTTCCAGAGCCGAGGAAACAGCGCAGACAGCTAGCCATTGAGCATAACAAGACACTTGCAGCCGATTCACAATTCTCAATACTCAATGAAAACGGCAAGGCAAACAATGTCATACACTCCTACCAAACAAGGAATATTTGTCGATATTGTTTGTTGCTCACATTCACTGTTGTTCAACAATGTGGCAGTATATCTTGGAGAATAGAGTGTATCGATCTGCGTGAACAGGAGCCTCTCAAGGAGCTGAGAGATGCCAGTCATGCCACTGCCAGCCACCCAAGGGCCAAGTGTTTGGATCAGCAATATATTCTGAGAGAAATGGAGCAGAACAAGAAAAAGGTATCGAAGGGACTATTGCAGAGTGATGACTTGTATCAGTATATCTTGGAGACTAGCGTGTATCCGCGTGAACCGGAGCCTCTCAAGGAGCTGAGAGAGGCCACTGCCAGCCACCCCGGGATCACTAGCTTGGTATTTTTCAGGGCTGGGATGGGTACTGCACCAGATGCAGGTCAGTTAATGACCATGCTATTGAAGTTAGTAGATGCAAAGAAGACGATTGAAGTTGGAGTTTTTACCGGATACTCTCTTCTCCTCACTGCTCTTACAATTCCTGAGGATGGCAAGATTGTCGCCATAGATATAGATCGTGAGCCATATGATCAAATAGGATTTCCAATCATAAAGAAAGCTGGTGTTGAGCACAAAATTGACCTCATTGAATCTGAGGCCCTACCCGGTCTTGATAAGCTATTGGAACAAGCAAACAAGGTTAACTATTGCAATTACCATGAGAGACTAATGAAGCTGCTGAAGGTGGGGGGTATAGCTGTGTATGATAACACTCTTTGGGGAGGGACAGTTGTAATGCCTGTTGAGTCGACTCCGGAATGGATGAAACCAAGCAGACAGTCTGTTATGGAGCTCAATAAGTTGCTCGCTGCTGATACTCGTGTCCAAATTTCGCATGCTGCTTTGGGTGATGGGATCACAGTTTATTATCAAAAACTTTATTGTGAATATTGCCTGAGAGAAATGGAGCACAACACGAAAAAGGTATCAAAGGGACTATTGCAGAATGATGAATTGTATCAGTATATCTTGGAGACTAGTGTGTATCCGCATGAACCGGAGCCTCTCAAGGAGCTTAGGGATGCCACTGCCAGCCACCCCAGGGCTGAGATGGCTACTGCACCAGATGCAGGCCAGTTGATGACCATGCTGTTGGGGCTAGTAGATGCAAAGAAGACGATTGAAGTTGGAGTTTTTACTGGATACTCTCTTCTCCTCACTGCTCTTGCAATTCCTGAGGATGGCAAGATTGTAGCCATAGATGTAAACCGTGAGCCATATGATCAAATAGGATTTCCAATCATAAAGAAAGCTGGTGTTGAGCACAAAATTGACCTCATTGAATCTGAGGCGCTGCCAGTTCTTGATAAGCTATTGGAACAACATGGAAATGAAGGAAGTTTTGACTTTGCTTTTGTCGATGCGGACAAGGTTAACTATTGGAATTACCATGAGAGACTAATGAAGCTGCTGAAGGTGGGAGGTATAGTTGTGTATGATAACACTCTTTGGGGAGGGTCAGTTGTAATGCCTATTGAGTCGACTCCAGAACACATGAAACCAGGCAGACAGTATTCTATAGAGCTCAACAAGTTACTTGCTGCTGATACTCGAGTCCAAATTTCACATGCTTCTTTGGGTGATGGGATCACAATTTGTAGGCGACTATACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0008150 GO:0008152 GO:0008168 GO:0008757 GO:0016740 GO:0016741 GO:0032259 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00360 ko00940 ko00941 ko00945 ko01100 ko01110 map00360 map00940 map00941 map00945 map01100 map01110

Pfam Domains

Protein Families

Methyltransf_3

Protein Analysis

774

Amino Acids

86.55

Weight (kDa)

5.53

Isoelectric Point (pI)

35.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Methyltransf_3	PF01596	24 - 219	6.7e-60	O-methyltransferase
Methyltransf_24	PF13578	73 - 180	3.3e-07	Methyltransferase domain
Methyltransf_3	PF01596	323 - 526	3e-57	O-methyltransferase
Methyltransf_3	PF01596	562 - 773	2.3e-75	O-methyltransferase
Methyltransf_24	PF13578	611 - 718	9.4e-09	Methyltransferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0003495)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G26220
fragaria_vesca	FvH4_1g06840 FvH4_1g06841 FvH4_1g06841
malus_domestica	MD02G1073100.v1.1 MD02G1073400.v1.1
prunus_persica	Prupe.7G214400_v2.0.a1 Prupe.7G214400_v2.0.a1 Prupe.7G214400_v2.0.a1
pyrus_communis	pycom02g05730 pycom02g05760 pycom02g05770
rosa_chinensis	RchiOBHm_Chr2g0092621 RchiOBHm_Chr2g0092631 RchiOBHm_Chr2g0093901 RchiOBHm_Chr2g0093911
rosa_laevigata	RLG00000016207 RLG00000016329
rosa_multiflora	Rmu_co8197646.1_g000001 Rmu_co8235837.1_g000001 Rmu_co8321945.1_g000001 Rmu_sc0003027.1_g000003
rosa_roxburghii	Rroxscaffold_2G00147620 Rroxscaffold_2G00147650 Rroxscaffold_2G00148920
rosa_rugosa	Rorug02G0028200 Rorug02G0028300
rosa_samantha	Rh2AG074000 Rh2AG074100 Rh2BG075100 Rh2BG075200 Rh2CG076700 Rh2CG076800
rosa_wichuraiana	Rw2G005890 Rw2G005900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1079
AccI	GTMKAC	2 cut(s)	1460, 2192
AccII	CGCG	1 cut(s)	985
AccIII	TCCGGA	1 cut(s)	1465
AciI	CCGC	3 cut(s)	983, 1700, 2075
AclWI	GGATC	5 cut(s)	562, 882, 1046, 1574, 2306
AcsI	RAATTY	2 cut(s)	1539, 2271
AcuI	CTGAAG	3 cut(s)	521, 1412, 2144
AcvI	CACGTG	1 cut(s)	89
AfaI	GTAC	2 cut(s)	393, 1075
AfiI	CCNNNNNNNGG	9 cut(s)	139, 143, 278, 859, 1088, 1199, 1753, 1781, 1892
AhlI	ACTAGT	2 cut(s)	74, 1688
AjnI	CCWGG	2 cut(s)	1751, 2212
Alw21I	GWGCWC	6 cut(s)	363, 1284, 1506, 1623, 1977, 2238
Alw26I	GTCTC	7 cut(s)	65, 305, 487, 962, 1369, 1679, 2101
AlwI	GGATC	5 cut(s)	562, 882, 1046, 1574, 2306
Ama87I	CYCGRG	3 cut(s)	138, 1034, 2262
Aor13HI	TCCGGA	1 cut(s)	1465
AoxI	GGCC	4 cut(s)	861, 1017, 1308, 1783
ApeKI	GCWGC	8 cut(s)	167, 638, 1387, 1520, 1550, 2005, 2119, 2252
ApoI	RAATTY	2 cut(s)	1539, 2271
ArsI	GACNNNNNNTTYG	2 cut(s)	2048, 2080
AspLEI	GCGC	5 cut(s)	100, 160, 262, 603, 2005
AspS9I	GGNCC	2 cut(s)	861, 1309
AsuC2I	CCSGG	3 cut(s)	1035, 1036, 1317
AsuHPI	GGTGA	2 cut(s)	1571, 2303
AsuNHI	GCTAGC	1 cut(s)	610
AvaI	CYCGRG	3 cut(s)	138, 1034, 2262
AxyI	CCTNAGG	3 cut(s)	273, 1194, 1887
BaeI	ACNNNNGTAYC	4 cut(s)	1518, 1551, 2250, 2283
BanII	GRGCYC	2 cut(s)	1506, 2238
BauI	CACGAG	2 cut(s)	488, 1530
BbrPI	CACGTG	1 cut(s)	89
BbsI	GAAGAC	3 cut(s)	218, 1139, 1832
Bbv12I	GWGCWC	6 cut(s)	363, 1284, 1506, 1623, 1977, 2238
BbvI	GCAGC	8 cut(s)	179, 650, 1374, 1507, 1537, 1992, 2106, 2239
BccI	CCATC	7 cut(s)	272, 1063, 1193, 1556, 1756, 1886, 2288
BceAI	ACGGC	2 cut(s)	167, 688
BcgI	CGANNNNNNTGC	2 cut(s)	1192, 1226
BciT130I	CCWGG	2 cut(s)	1753, 2214
BciVI	GTATCC	5 cut(s)	93, 990, 1154, 1707, 1847
BclI	TGATCA	3 cut(s)	319, 1240, 1933
BcnI	CCSGG	3 cut(s)	1035, 1036, 1317
BcoDI	GTCTC	7 cut(s)	65, 305, 487, 962, 1369, 1679, 2101
BcuI	ACTAGT	2 cut(s)	74, 1688
BfaI	CTAG	7 cut(s)	75, 179, 611, 972, 1044, 1689, 1811
BfmI	CTRYAG	2 cut(s)	547, 2229
BfoI	RGCGCY	2 cut(s)	101, 2006
BfuAI	ACCTGC	1 cut(s)	1079
BfuI	GTATCC	5 cut(s)	93, 990, 1154, 1707, 1847
BglII	AGATCT	1 cut(s)	21
BisI	GCNGC	8 cut(s)	168, 639, 1388, 1521, 1551, 2006, 2120, 2253
BlsI	GCNGC	8 cut(s)	169, 640, 1389, 1522, 1552, 2007, 2121, 2254
Bme1390I	CCNGG	5 cut(s)	1035, 1036, 1317, 1753, 2214
BmeT110I	CYCGRG	3 cut(s)	138, 1034, 2262
BmgT120I	GGNCC	2 cut(s)	861, 1309
BmiI	GGNNCC	3 cut(s)	810, 996, 1713
BmrFI	CCNGG	5 cut(s)	1035, 1036, 1317, 1753, 2214
BmtI	GCTAGC	1 cut(s)	614
BpiI	GAAGAC	3 cut(s)	218, 1139, 1832
BpmI	CTGGAG	1 cut(s)	2181
Bpu10I	CCTNAGC	2 cut(s)	113, 1727
BpuEI	CTTGAG	4 cut(s)	89, 800, 986, 1703
BpuMI	CCSGG	3 cut(s)	1035, 1036, 1317
Bsa29I	ATCGAT	1 cut(s)	792
BsaAI	YACGTR	1 cut(s)	89
BsaBI	GATNNNNATC	3 cut(s)	20, 303, 1224
BsaJI	CCNNGG	7 cut(s)	137, 590, 857, 1033, 1034, 1751, 1752
BsaWI	WCCGGW	4 cut(s)	991, 1157, 1465, 1708
BsaXI	ACNNNNNCTCC	6 cut(s)	1425, 1455, 2124, 2154, 2157, 2187
Bsc4I	CCNNNNNNNGG	9 cut(s)	139, 143, 278, 859, 1088, 1199, 1753, 1781, 1892
Bse1I	ACTGG	5 cut(s)	395, 834, 1786, 1855, 2009
Bse21I	CCTNAGG	3 cut(s)	273, 1194, 1887
Bse8I	GATNNNNATC	3 cut(s)	20, 303, 1224
BseAI	TCCGGA	1 cut(s)	1465
BseBI	CCWGG	2 cut(s)	1753, 2214
BseCI	ATCGAT	1 cut(s)	792
BseDI	CCNNGG	7 cut(s)	137, 590, 857, 1033, 1034, 1751, 1752
BseGI	GGATG	9 cut(s)	124, 283, 418, 463, 1074, 1204, 1479, 1738, 1897
BseJI	GATNNNNATC	3 cut(s)	20, 303, 1224
BseLI	CCNNNNNNNGG	9 cut(s)	139, 143, 278, 859, 1088, 1199, 1753, 1781, 1892
BseNI	ACTGG	5 cut(s)	395, 834, 1786, 1855, 2009
BseRI	GAGGAG	3 cut(s)	242, 1163, 1856
BseXI	GCAGC	8 cut(s)	179, 650, 1374, 1507, 1537, 1992, 2106, 2239
BseYI	CCCAGC	1 cut(s)	1064
BsgI	GTGCAG	2 cut(s)	1062, 1755
Bsh1236I	CGCG	1 cut(s)	985
BshFI	GGCC	4 cut(s)	863, 1019, 1310, 1785
BshVI	ATCGAT	1 cut(s)	792
BsiHKAI	GWGCWC	6 cut(s)	363, 1284, 1506, 1623, 1977, 2238
BsiHKCI	CYCGRG	3 cut(s)	138, 1034, 2262
BsiSI	CCGG	6 cut(s)	992, 1035, 1158, 1317, 1466, 1709
BslFI	GGGAC	3 cut(s)	942, 1450, 1659
BslI	CCNNNNNNNGG	9 cut(s)	139, 143, 278, 859, 1088, 1199, 1753, 1781, 1892
BsmAI	GTCTC	7 cut(s)	65, 305, 487, 962, 1369, 1679, 2101
BsmFI	GGGAC	3 cut(s)	942, 1450, 1659
BsnI	GGCC	4 cut(s)	863, 1019, 1310, 1785
BsoBI	CYCGRG	3 cut(s)	138, 1034, 2262
Bsp1286I	GDGCHC	6 cut(s)	363, 1284, 1506, 1623, 1977, 2238
Bsp13I	TCCGGA	1 cut(s)	1465
BspACI	CCGC	3 cut(s)	983, 1700, 2075
BspANI	GGCC	4 cut(s)	863, 1019, 1310, 1785
BspDI	ATCGAT	1 cut(s)	792
BspEI	TCCGGA	1 cut(s)	1465
BspFNI	CGCG	1 cut(s)	985
BspLI	GGNNCC	3 cut(s)	810, 996, 1713
BspMAI	CTGCAG	1 cut(s)	551
BspMI	ACCTGC	1 cut(s)	1079
BspOI	GCTAGC	1 cut(s)	614
BspPI	GGATC	5 cut(s)	562, 882, 1046, 1574, 2306
BsrI	ACTGG	5 cut(s)	395, 834, 1786, 1855, 2009
BssECI	CCNNGG	7 cut(s)	137, 590, 857, 1033, 1034, 1751, 1752
BssSI	CACGAG	2 cut(s)	488, 1530
BssT1I	CCWWGG	1 cut(s)	857
Bst2BI	CACGAG	2 cut(s)	488, 1530
Bst2UI	CCWGG	2 cut(s)	1753, 2214
Bst4CI	ACNGT	6 cut(s)	748, 1441, 1491, 1573, 1922, 2223
Bst6I	CTCTTC	3 cut(s)	252, 1173, 1866
BstAPI	GCANNNNNTGC	2 cut(s)	1085, 1778
BstBAI	YACGTR	1 cut(s)	89
BstC8I	GCNNGC	9 cut(s)	130, 332, 612, 850, 1027, 1518, 1548, 1744, 1783
BstF5I	GGATG	9 cut(s)	124, 283, 418, 463, 1074, 1204, 1479, 1738, 1897
BstFNI	CGCG	1 cut(s)	985
BstH2I	RGCGCY	2 cut(s)	101, 2006
BstHHI	GCGC	5 cut(s)	100, 160, 262, 603, 2005
BstMAI	GTCTC	7 cut(s)	65, 305, 487, 962, 1369, 1679, 2101
BstMWI	GCNNNNNNNGC	9 cut(s)	149, 164, 193, 607, 839, 1085, 1763, 1778, 1807
BstNI	CCWGG	2 cut(s)	1753, 2214
BstNSI	RCATGY	2 cut(s)	1550, 2282
BstSCI	CCNGG	5 cut(s)	1033, 1034, 1315, 1751, 2212
BstSFI	CTRYAG	2 cut(s)	547, 2229
BstUI	CGCG	1 cut(s)	985
BstV1I	GCAGC	8 cut(s)	179, 650, 1374, 1507, 1537, 1992, 2106, 2239
BstV2I	GAAGAC	3 cut(s)	218, 1139, 1832
BstX2I	RGATCY	1 cut(s)	21
BstXI	CCANNNNNNTGG	2 cut(s)	871, 1804
BstYI	RGATCY	1 cut(s)	21
Bsu15I	ATCGAT	1 cut(s)	792
Bsu36I	CCTNAGG	3 cut(s)	273, 1194, 1887
BsuI	GTATCC	5 cut(s)	93, 990, 1154, 1707, 1847
BsuRI	GGCC	4 cut(s)	863, 1019, 1310, 1785
BsuTUI	ATCGAT	1 cut(s)	792
BtsCI	GGATG	9 cut(s)	124, 283, 418, 463, 1074, 1204, 1479, 1738, 1897
BtsI	GCAGTG	7 cut(s)	123, 255, 843, 1020, 1176, 1737, 1869
BtsIMutI	CAGTG	8 cut(s)	123, 255, 744, 843, 1020, 1176, 1737, 1869
BveI	ACCTGC	1 cut(s)	1079
Cac8I	GCNNGC	9 cut(s)	130, 332, 612, 850, 1027, 1518, 1548, 1744, 1783
CfoI	GCGC	5 cut(s)	100, 160, 262, 603, 2005
Cfr13I	GGNCC	2 cut(s)	861, 1309
Cfr9I	CCCGGG	1 cut(s)	1034
ClaI	ATCGAT	1 cut(s)	792
Csp6I	GTAC	2 cut(s)	392, 1074
CviQI	GTAC	2 cut(s)	392, 1074
Eam1104I	CTCTTC	3 cut(s)	252, 1173, 1866
EarI	CTCTTC	3 cut(s)	252, 1173, 1866
Ecl136II	GAGCTC	2 cut(s)	1504, 2236
Eco130I	CCWWGG	1 cut(s)	857
Eco24I	GRGCYC	2 cut(s)	1506, 2238
Eco53kI	GAGCTC	2 cut(s)	1504, 2236
Eco57I	CTGAAG	3 cut(s)	521, 1412, 2144
Eco72I	CACGTG	1 cut(s)	89
Eco81I	CCTNAGG	3 cut(s)	273, 1194, 1887
Eco88I	CYCGRG	3 cut(s)	138, 1034, 2262
EcoICRI	GAGCTC	2 cut(s)	1504, 2236
EcoO109I	RGGNCCY	1 cut(s)	1309
EcoRII	CCWGG	2 cut(s)	1751, 2212
EcoT14I	CCWWGG	1 cut(s)	857
EcoT38I	GRGCYC	2 cut(s)	1506, 2238
ErhI	CCWWGG	1 cut(s)	857
FaqI	GGGAC	3 cut(s)	942, 1450, 1659
FauNDI	CATATG	3 cut(s)	316, 1237, 1930
FbaI	TGATCA	3 cut(s)	319, 1240, 1933
FblI	GTMKAC	2 cut(s)	1460, 2192
Fnu4HI	GCNGC	8 cut(s)	168, 639, 1388, 1521, 1551, 2006, 2120, 2253
FokI	GGATG	9 cut(s)	131, 290, 405, 470, 1081, 1211, 1486, 1745, 1904
FriOI	GRGCYC	2 cut(s)	1506, 2238
Fsp4HI	GCNGC	8 cut(s)	168, 639, 1388, 1521, 1551, 2006, 2120, 2253
FspBI	CTAG	7 cut(s)	75, 179, 611, 972, 1044, 1689, 1811
GlaI	GCGC	5 cut(s)	99, 159, 261, 602, 2004
GluI	GCNGC	8 cut(s)	168, 639, 1388, 1521, 1551, 2006, 2120, 2253
GsaI	CCCAGC	1 cut(s)	1068
GsuI	CTGGAG	1 cut(s)	2181
HaeII	RGCGCY	2 cut(s)	101, 2006
HaeIII	GGCC	4 cut(s)	863, 1019, 1310, 1785
HapII	CCGG	6 cut(s)	992, 1035, 1158, 1317, 1466, 1709
HhaI	GCGC	5 cut(s)	100, 160, 262, 603, 2005
Hin6I	GCGC	5 cut(s)	98, 158, 260, 601, 2003
HinP1I	GCGC	5 cut(s)	98, 158, 260, 601, 2003
HincII	GTYRAC	5 cut(s)	472, 1354, 1461, 2086, 2193
HindII	GTYRAC	5 cut(s)	472, 1354, 1461, 2086, 2193
HpaI	GTTAAC	3 cut(s)	472, 1354, 2086
HpaII	CCGG	6 cut(s)	992, 1035, 1158, 1317, 1466, 1709
HphI	GGTGA	2 cut(s)	1571, 2303
Hpy166II	GTNNAC	9 cut(s)	92, 472, 803, 989, 1354, 1461, 1918, 2086, 2193
Hpy188I	TCNGA	5 cut(s)	385, 442, 891, 1306, 1999
Hpy8I	GTNNAC	9 cut(s)	92, 472, 803, 989, 1354, 1461, 1918, 2086, 2193
HpyAV	CCTTC	6 cut(s)	425, 505, 919, 1387, 2039, 2119
HpyCH4III	ACNGT	6 cut(s)	748, 1441, 1491, 1573, 1922, 2223
HpyCH4IV	ACGT	1 cut(s)	88
HpyF10VI	GCNNNNNNNGC	9 cut(s)	149, 164, 193, 607, 839, 1085, 1763, 1778, 1807
HpySE526I	ACGT	1 cut(s)	88
HspAI	GCGC	5 cut(s)	98, 158, 260, 601, 2003
Kpn2I	TCCGGA	1 cut(s)	1465
Ksp22I	TGATCA	3 cut(s)	319, 1240, 1933
KspAI	GTTAAC	3 cut(s)	472, 1354, 2086
Lsp1109I	GCAGC	8 cut(s)	179, 650, 1374, 1507, 1537, 1992, 2106, 2239
MaeI	CTAG	7 cut(s)	75, 179, 611, 972, 1044, 1689, 1811
MaeII	ACGT	1 cut(s)	88
MaeIII	GTNAC	2 cut(s)	174, 2244
MboII	GAAGA	6 cut(s)	223, 239, 1144, 1160, 1837, 1853
MfeI	CAATTG	2 cut(s)	216, 475
MflI	RGATCY	1 cut(s)	21
MhlI	GDGCHC	6 cut(s)	363, 1284, 1506, 1623, 1977, 2238
MlyI	GAGTC	5 cut(s)	1456, 1466, 2188, 2198, 2274
MmeI	TCCRAC	2 cut(s)	1126, 1819
MroI	TCCGGA	1 cut(s)	1465
MseI	TTAA	5 cut(s)	18, 471, 1097, 1353, 2085
MslI	CAYNNNNRTG	1 cut(s)	2040
MspI	CCGG	6 cut(s)	992, 1035, 1158, 1317, 1466, 1709
MspR9I	CCNGG	5 cut(s)	1035, 1036, 1317, 1753, 2214
MunI	CAATTG	2 cut(s)	216, 475
MvaI	CCWGG	2 cut(s)	1753, 2214
MvnI	CGCG	1 cut(s)	985
MwoI	GCNNNNNNNGC	9 cut(s)	149, 164, 193, 607, 839, 1085, 1763, 1778, 1807
NciI	CCSGG	3 cut(s)	1035, 1036, 1317
NdeI	CATATG	3 cut(s)	316, 1237, 1930
NheI	GCTAGC	1 cut(s)	610
NlaIV	GGNNCC	3 cut(s)	810, 996, 1713
NmeAIII	GCCGAG	1 cut(s)	615
NspI	RCATGY	2 cut(s)	1550, 2282
PaeI	GCATGC	1 cut(s)	1550
PaeR7I	CTCGAG	1 cut(s)	2262
PasI	CCCWGGG	1 cut(s)	1752
PfeI	GAWTC	5 cut(s)	5, 380, 644, 1301, 1994
PkrI	GCNGC	8 cut(s)	169, 640, 1389, 1522, 1552, 2007, 2121, 2254
PleI	GAGTC	5 cut(s)	1456, 1465, 2188, 2197, 2273
PmaCI	CACGTG	1 cut(s)	89
PmlI	CACGTG	1 cut(s)	89
PpsI	GAGTC	5 cut(s)	1456, 1465, 2188, 2197, 2273
Ppu21I	YACGTR	1 cut(s)	89
Psp124BI	GAGCTC	2 cut(s)	1506, 2238
Psp6I	CCWGG	2 cut(s)	1751, 2212
PspCI	CACGTG	1 cut(s)	89
PspFI	CCCAGC	1 cut(s)	1064
PspGI	CCWGG	2 cut(s)	1751, 2212
PspN4I	GGNNCC	3 cut(s)	810, 996, 1713
PspPI	GGNCC	2 cut(s)	861, 1309
PspXI	VCTCGAGB	1 cut(s)	2262
PstI	CTGCAG	1 cut(s)	551
PsuI	RGATCY	1 cut(s)	21
RsaI	GTAC	2 cut(s)	393, 1075
RsaNI	GTAC	2 cut(s)	392, 1074
RseI	CAYNNNNRTG	1 cut(s)	2040
SacI	GAGCTC	2 cut(s)	1506, 2238
SalI	GTCGAC	2 cut(s)	1459, 2191
SaqAI	TTAA	5 cut(s)	18, 471, 1097, 1353, 2085
SatI	GCNGC	8 cut(s)	168, 639, 1388, 1521, 1551, 2006, 2120, 2253
Sau96I	GGNCC	2 cut(s)	861, 1309
SchI	GAGTC	5 cut(s)	1456, 1466, 2188, 2198, 2274
ScrFI	CCNGG	5 cut(s)	1035, 1036, 1317, 1753, 2214
SduI	GDGCHC	6 cut(s)	363, 1284, 1506, 1623, 1977, 2238
SfcI	CTRYAG	2 cut(s)	547, 2229
Sfr274I	CTCGAG	1 cut(s)	2262
SlaI	CTCGAG	1 cut(s)	2262
SmaI	CCCGGG	1 cut(s)	1036
SmiMI	CAYNNNNRTG	1 cut(s)	2040
SmlI	CTYRAG	5 cut(s)	104, 815, 1001, 1718, 2262
SmoI	CTYRAG	5 cut(s)	104, 815, 1001, 1718, 2262
SpeI	ACTAGT	2 cut(s)	74, 1688
SphI	GCATGC	1 cut(s)	1550
SsiI	CCGC	3 cut(s)	983, 1700, 2075
SspI	AATATT	2 cut(s)	715, 1601
SspMI	CTAG	7 cut(s)	75, 179, 611, 972, 1044, 1689, 1811
SstI	GAGCTC	2 cut(s)	1506, 2238
StyD4I	CCNGG	5 cut(s)	1033, 1034, 1315, 1751, 2212
StyI	CCWWGG	1 cut(s)	857
TaaI	ACNGT	6 cut(s)	748, 1441, 1491, 1573, 1922, 2223
TaiI	ACGT	1 cut(s)	91
TaqI	TCGA	7 cut(s)	721, 792, 923, 1460, 2070, 2192, 2263
TfiI	GAWTC	5 cut(s)	5, 380, 644, 1301, 1994
Tru1I	TTAA	5 cut(s)	18, 471, 1097, 1353, 2085
Tru9I	TTAA	5 cut(s)	18, 471, 1097, 1353, 2085
TscAI	CASTG	8 cut(s)	130, 262, 751, 850, 1027, 1183, 1744, 1876
TseI	GCWGC	8 cut(s)	167, 638, 1387, 1520, 1550, 2005, 2119, 2252
TspMI	CCCGGG	1 cut(s)	1034
TspRI	CASTG	8 cut(s)	130, 262, 751, 850, 1027, 1183, 1744, 1876
XapI	RAATTY	2 cut(s)	1539, 2271
XceI	RCATGY	2 cut(s)	1550, 2282
XcmI	CCANNNNNNNNNTGG	2 cut(s)	140, 1759
XhoI	CTCGAG	1 cut(s)	2262
XmaI	CCCGGG	1 cut(s)	1034
XmiI	GTMKAC	2 cut(s)	1460, 2192
XspI	CTAG	7 cut(s)	75, 179, 611, 972, 1044, 1689, 1811

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.