Rroxscaffold_2G00147620

caffeoyl-CoA O-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0003495
Physical Location & Seq
Reverse (-)
85378755 .. 85380584
1830 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00147620.1

Sequence Viewer

Length: 303 bp
ATGGAGCACAACACGAGAAAGGTATCAAAGGGACTATTGCAGAATGATGAATTGTATCGGTATATCTTGGAGACTAGTGTGTATCCGCGTGAACCAGAGCTTCTCAAGGAGCTTAGGGATGCCACTGTCAGCCACCCCAGGGCTGAGATGGCTGCTGCACCAGATGCAGGCCAGTTGATGACCATGCTGTTGGGGCTAGTAGATGCAAAGAAGATGATTGAAGTAGGAGTTTGTACAGGATACTCTCTTCTCCTCACTGCTCTTGCAATTCCTGAGGATGGCAAGCATAGATGCAAACCGTGA

Protein Analysis

100

Amino Acids

11.15

Weight (kDa)

6.28

Isoelectric Point (pI)

25.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_3 PF01596 24 - 95 2.4e-21 O-methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 88
AciI CCGC 1 cut(s) 86
AfaI GTAC 1 cut(s) 235
AfiI CCNNNNNNNGG 3 cut(s) 139, 167, 278
AgsI TTSAA 1 cut(s) 221
AhlI ACTAGT 1 cut(s) 74
AjnI CCWGG 1 cut(s) 137
AluBI AGCT 2 cut(s) 100, 112
AluI AGCT 2 cut(s) 100, 112
Alw21I GWGCWC 1 cut(s) 9
Alw26I GTCTC 1 cut(s) 65
AoxI GGCC 1 cut(s) 169
ApeKI GCWGC 2 cut(s) 152, 155
AxyI CCTNAGG 1 cut(s) 273
BauI CACGAG 1 cut(s) 13
Bbv12I GWGCWC 1 cut(s) 9
BbvI GCAGC 2 cut(s) 139, 142
BccI CCATC 2 cut(s) 142, 272
BciT130I CCWGG 1 cut(s) 139
BciVI GTATCC 2 cut(s) 93, 233
BcoDI GTCTC 1 cut(s) 65
BcuI ACTAGT 1 cut(s) 74
BfaI CTAG 2 cut(s) 75, 197
BfuI GTATCC 2 cut(s) 93, 233
BisI GCNGC 2 cut(s) 153, 156
BlsI GCNGC 2 cut(s) 154, 157
Bme1390I CCNGG 1 cut(s) 139
BmrFI CCNGG 1 cut(s) 139
BmsI GCATC 4 cut(s) 109, 154, 193, 281
Bpu10I CCTNAGC 1 cut(s) 113
BpuEI CTTGAG 1 cut(s) 89
BsaJI CCNNGG 2 cut(s) 137, 138
Bsc4I CCNNNNNNNGG 3 cut(s) 139, 167, 278
Bse1I ACTGG 1 cut(s) 172
Bse21I CCTNAGG 1 cut(s) 273
BseBI CCWGG 1 cut(s) 139
BseDI CCNNGG 2 cut(s) 137, 138
BseGI GGATG 2 cut(s) 124, 283
BseLI CCNNNNNNNGG 3 cut(s) 139, 167, 278
BseMII CTCAG 2 cut(s) 135, 264
BseNI ACTGG 1 cut(s) 172
BseRI GAGGAG 1 cut(s) 242
BseXI GCAGC 2 cut(s) 139, 142
BsgI GTGCAG 1 cut(s) 141
Bsh1236I CGCG 1 cut(s) 88
BshFI GGCC 1 cut(s) 171
BsiHKAI GWGCWC 1 cut(s) 9
BslFI GGGAC 1 cut(s) 45
BslI CCNNNNNNNGG 3 cut(s) 139, 167, 278
BsmAI GTCTC 1 cut(s) 65
BsmFI GGGAC 1 cut(s) 45
BsnI GGCC 1 cut(s) 171
Bsp1286I GDGCHC 1 cut(s) 9
Bsp1407I TGTACA 1 cut(s) 233
BspACI CCGC 1 cut(s) 86
BspANI GGCC 1 cut(s) 171
BspCNI CTCAG 2 cut(s) 136, 265
BspFNI CGCG 1 cut(s) 88
BsrGI TGTACA 1 cut(s) 233
BsrI ACTGG 1 cut(s) 172
BssECI CCNNGG 2 cut(s) 137, 138
BssSI CACGAG 1 cut(s) 13
Bst2BI CACGAG 1 cut(s) 13
Bst2UI CCWGG 1 cut(s) 139
Bst4CI ACNGT 2 cut(s) 127, 300
Bst6I CTCTTC 1 cut(s) 252
BstAPI GCANNNNNTGC 1 cut(s) 164
BstAUI TGTACA 1 cut(s) 233
BstC8I GCNNGC 2 cut(s) 169, 284
BstDEI CTNAG 3 cut(s) 113, 144, 273
BstF5I GGATG 2 cut(s) 124, 283
BstFNI CGCG 1 cut(s) 88
BstMAI GTCTC 1 cut(s) 65
BstMWI GCNNNNNNNGC 3 cut(s) 149, 164, 193
BstNI CCWGG 1 cut(s) 139
BstSCI CCNGG 1 cut(s) 137
BstUI CGCG 1 cut(s) 88
BstV1I GCAGC 2 cut(s) 139, 142
BstXI CCANNNNNNTGG 1 cut(s) 190
Bsu36I CCTNAGG 1 cut(s) 273
BsuI GTATCC 2 cut(s) 93, 233
BsuRI GGCC 1 cut(s) 171
BtsCI GGATG 2 cut(s) 124, 283
BtsI GCAGTG 1 cut(s) 255
BtsIMutI CAGTG 2 cut(s) 123, 255
Cac8I GCNNGC 2 cut(s) 169, 284
Csp6I GTAC 1 cut(s) 234
CviAII CATG 1 cut(s) 184
CviJI RGCY 7 cut(s) 100, 112, 132, 143, 152, 171, 196
CviKI_1 RGCY 7 cut(s) 100, 112, 132, 143, 152, 171, 196
CviQI GTAC 1 cut(s) 234
DdeI CTNAG 3 cut(s) 113, 144, 273
Eam1104I CTCTTC 1 cut(s) 252
EarI CTCTTC 1 cut(s) 252
Eco81I CCTNAGG 1 cut(s) 273
EcoRII CCWGG 1 cut(s) 137
FaeI CATG 1 cut(s) 187
FaiI YATR 3 cut(s) 63, 185, 288
FaqI GGGAC 1 cut(s) 45
FatI CATG 1 cut(s) 183
Fnu4HI GCNGC 2 cut(s) 153, 156
FokI GGATG 2 cut(s) 131, 290
Fsp4HI GCNGC 2 cut(s) 153, 156
FspBI CTAG 2 cut(s) 75, 197
GluI GCNGC 2 cut(s) 153, 156
HaeIII GGCC 1 cut(s) 171
Hin1II CATG 1 cut(s) 187
Hpy166II GTNNAC 1 cut(s) 92
Hpy188III TCNNGA 1 cut(s) 272
Hpy8I GTNNAC 1 cut(s) 92
HpyCH4III ACNGT 2 cut(s) 127, 300
HpyCH4V TGCA 6 cut(s) 40, 158, 167, 206, 266, 294
HpyF10VI GCNNNNNNNGC 3 cut(s) 149, 164, 193
HpyF3I CTNAG 3 cut(s) 113, 144, 273
Hsp92II CATG 1 cut(s) 187
LmnI GCTCC 2 cut(s) 4, 109
LpnPI CCDG 8 cut(s) 108, 124, 151, 153, 174, 185, 222, 285
Lsp1109I GCAGC 2 cut(s) 139, 142
LweI GCATC 4 cut(s) 109, 154, 193, 281
MaeI CTAG 2 cut(s) 75, 197
MboII GAAGA 2 cut(s) 223, 239
MhlI GDGCHC 1 cut(s) 9
MluCI AATT 2 cut(s) 50, 267
MnlI CCTC 2 cut(s) 263, 268
MspR9I CCNGG 1 cut(s) 139
MvaI CCWGG 1 cut(s) 139
MvnI CGCG 1 cut(s) 88
MwoI GCNNNNNNNGC 3 cut(s) 149, 164, 193
NlaIII CATG 1 cut(s) 187
PasI CCCWGGG 1 cut(s) 138
PkrI GCNGC 2 cut(s) 154, 157
Psp6I CCWGG 1 cut(s) 137
PspGI CCWGG 1 cut(s) 137
RsaI GTAC 1 cut(s) 235
RsaNI GTAC 1 cut(s) 234
SatI GCNGC 2 cut(s) 153, 156
ScrFI CCNGG 1 cut(s) 139
SduI GDGCHC 1 cut(s) 9
SetI ASST 3 cut(s) 24, 102, 114
SfaNI GCATC 4 cut(s) 109, 154, 193, 281
SmlI CTYRAG 1 cut(s) 104
SmoI CTYRAG 1 cut(s) 104
SpeI ACTAGT 1 cut(s) 74
Sse9I AATT 2 cut(s) 50, 267
SsiI CCGC 1 cut(s) 86
SspMI CTAG 2 cut(s) 75, 197
StyD4I CCNGG 1 cut(s) 137
TaaI ACNGT 2 cut(s) 127, 300
TasI AATT 2 cut(s) 50, 267
TatI WGTACW 1 cut(s) 233
TscAI CASTG 2 cut(s) 130, 262
TseI GCWGC 2 cut(s) 152, 155
TspDTI ATGAA 1 cut(s) 63
TspRI CASTG 2 cut(s) 130, 262
XcmI CCANNNNNNNNNTGG 1 cut(s) 145
XspI CTAG 2 cut(s) 75, 197
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.