MD02G1308900.v1.1

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0002545
Physical Location & Seq
Forward (+)
36413637 .. 36415326
1690 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1308900.v1.1.491

Sequence Viewer

Length: 354 bp
ATGGGTGTGATTTCAAGGCAAGTGTTCCCTGTTTGTGAAAGCCTGTGTTTCTTCTGTCCAGCATTGCGTGCGAGATCACGGCATCCCGTCAAACGATACAAGCAGATGCTCGCCGAGATATTTCCTCGCTCTCCGGACGAAGAACCAAACGATCGCAAGATTAGTAAACTATGTGAATATGCTTCAAAAACTCCTCTCCGTATTCCAAAGATCACAACTACTCTGGAGCAACGGTGTTACAAGGATTTGAGGACTGAAAACTTTCACTCAGTGAAAGTTGTCATGTGCATCTACCGGAAGTTGTTGGTCTCTTGTAAGGAGCAAATATTCCAAAACATTATTGAGAAAGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

118

Amino Acids

13.78

Weight (kDa)

9.51

Isoelectric Point (pI)

61.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EFR3_ARM PF21052 65 - 115 1.2e-07 EFR3, armadillo repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 133
AdeI CACNNNGTG 1 cut(s) 271
AgsI TTSAA 2 cut(s) 15, 186
Alw26I GTCTC 1 cut(s) 313
Aor13HI TCCGGA 1 cut(s) 133
Asp700I GAANNNNTTC 1 cut(s) 261
BceAI ACGGC 1 cut(s) 95
BcoDI GTCTC 1 cut(s) 313
BfaI CTAG 1 cut(s) 352
BmsI GCATC 3 cut(s) 91, 96, 297
BpmI CTGGAG 1 cut(s) 245
BsaI GGTCTC 1 cut(s) 313
BsaWI WCCGGW 2 cut(s) 133, 294
BsaXI ACNNNNNCTCC 2 cut(s) 218, 248
Bse3DI GCAATG 1 cut(s) 62
BseAI TCCGGA 1 cut(s) 133
BseGI GGATG 1 cut(s) 82
BseMI GCAATG 1 cut(s) 62
BseMII CTCAG 1 cut(s) 282
BseRI GAGGAG 1 cut(s) 183
Bsh1285I CGRYCG 1 cut(s) 154
BsiEI CGRYCG 1 cut(s) 154
BsiSI CCGG 2 cut(s) 134, 295
BsmAI GTCTC 1 cut(s) 313
Bso31I GGTCTC 1 cut(s) 313
Bsp13I TCCGGA 1 cut(s) 133
Bsp143I GATC 3 cut(s) 74, 151, 210
BspCNI CTCAG 1 cut(s) 281
BspEI TCCGGA 1 cut(s) 133
BspTNI GGTCTC 1 cut(s) 313
BsrDI GCAATG 1 cut(s) 62
BssMI GATC 3 cut(s) 74, 151, 210
Bst4CI ACNGT 1 cut(s) 234
BstAPI GCANNNNNTGC 1 cut(s) 68
BstC8I GCNNGC 2 cut(s) 69, 111
BstDEI CTNAG 1 cut(s) 268
BstF5I GGATG 1 cut(s) 82
BstKTI GATC 3 cut(s) 77, 154, 213
BstMAI GTCTC 1 cut(s) 313
BstMBI GATC 3 cut(s) 74, 151, 210
BstMCI CGRYCG 1 cut(s) 154
BstMWI GCNNNNNNNGC 1 cut(s) 68
BtsCI GGATG 1 cut(s) 82
BtsIMutI CAGTG 1 cut(s) 276
Cac8I GCNNGC 2 cut(s) 69, 111
CviAII CATG 1 cut(s) 283
CviJI RGCY 2 cut(s) 42, 350
CviKI_1 RGCY 2 cut(s) 42, 350
DdeI CTNAG 1 cut(s) 268
DpnI GATC 3 cut(s) 76, 153, 212
DpnII GATC 3 cut(s) 74, 151, 210
DraIII CACNNNGTG 1 cut(s) 271
Eco31I GGTCTC 1 cut(s) 313
FaeI CATG 1 cut(s) 286
FaiI YATR 3 cut(s) 172, 180, 284
FatI CATG 1 cut(s) 282
FokI GGATG 1 cut(s) 69
FspBI CTAG 1 cut(s) 352
GsuI CTGGAG 1 cut(s) 245
HapII CCGG 2 cut(s) 134, 295
Hin1II CATG 1 cut(s) 286
HpaII CCGG 2 cut(s) 134, 295
Hpy166II GTNNAC 1 cut(s) 167
Hpy188III TCNNGA 2 cut(s) 134, 224
Hpy8I GTNNAC 1 cut(s) 167
HpyCH4III ACNGT 1 cut(s) 234
HpyCH4V TGCA 1 cut(s) 288
HpyF10VI GCNNNNNNNGC 1 cut(s) 68
HpyF3I CTNAG 1 cut(s) 268
Hsp92II CATG 1 cut(s) 286
Kpn2I TCCGGA 1 cut(s) 133
Kzo9I GATC 3 cut(s) 74, 151, 210
LmnI GCTCC 2 cut(s) 226, 319
LpnPI CCDG 6 cut(s) 42, 56, 72, 147, 209, 308
LweI GCATC 3 cut(s) 91, 96, 297
MaeI CTAG 1 cut(s) 352
MaeIII GTNAC 1 cut(s) 236
MalI GATC 3 cut(s) 76, 153, 212
MboI GATC 3 cut(s) 74, 151, 210
MboII GAAGA 2 cut(s) 43, 152
MnlI CCTC 3 cut(s) 135, 204, 243
MroI TCCGGA 1 cut(s) 133
MroXI GAANNNNTTC 1 cut(s) 261
MspI CCGG 2 cut(s) 134, 295
MwoI GCNNNNNNNGC 1 cut(s) 68
NdeII GATC 3 cut(s) 74, 151, 210
NlaIII CATG 1 cut(s) 286
NmeAIII GCCGAG 1 cut(s) 139
PdmI GAANNNNTTC 1 cut(s) 261
Ple19I CGATCG 1 cut(s) 154
PvuI CGATCG 1 cut(s) 154
Sau3AI GATC 3 cut(s) 74, 151, 210
SfaNI GCATC 3 cut(s) 91, 96, 297
SspI AATATT 1 cut(s) 327
SspMI CTAG 1 cut(s) 352
TaaI ACNGT 1 cut(s) 234
TscAI CASTG 1 cut(s) 276
TspGWI ACGGA 1 cut(s) 188
TspRI CASTG 1 cut(s) 276
XmnI GAANNNNTTC 1 cut(s) 261
XspI CTAG 1 cut(s) 352
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.