Rh3DG271900

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0002545
Physical Location & Seq
Forward (+)
27246280 .. 27258001
11722 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG271900.1

Sequence Viewer

Length: 1398 bp
ATGAGCGCGGTTTCGGGTGTAGTGTCTCGCCAAGTTTTGCCTGCTTGCGGCAGTCTCTGTTTCTTTTGCCCCGGGCTGCGTGCCAGGTCCCGGCAGCCCGTCAAGAGGTACAAGAAGCTCATCGCTGATATTTTCCCCAAAAACCAGGAAGAAGGACCAAATGATCGGAAGATAGGAAAATTGTGTGAATATGCTGCTAAAAATCCTTTGCGGATTCCAAAGATCACAACCTCTCTTGAGCAAAGGTGTTACAAAGAATTGAGAAATGAGAATTTTCGATCCACAAAAATTGTCATGTGCATTTACAAGAAATTGCTGATAACTTGCAAGGAGCAAATGCCTCTCTTTGCGAGTAGCTTGCTCAGCATTATGCATACCCTTCTGGATCAATCACGCCAAGATGAAATGCAAATTATAGGATGCCAGACTCTTTTTGACTTTGTAAATAATCAGGTGGATGGAACATACATGTTTAACTTAGAAGGATTTATTCCGAAACTCTGTCAGATAGCTCAAGAGCCAGGGGAAGATGAAAGGGCAAATAATTTACGTTGTGCTGCACTGCAAGCCCTTTCTTCAATGATTGTTGCAGTGGTCTTGGAAAACTATGGAGGTTTTAAGGAAGCTGAAGGTTCGCAGATTCAGTGGGTGCAGGAAGTTCAAAAAGATGAGGGTCATGTTTCTCCACAAGATGTCAGAATCAGGATTCCTCCTTGGAGTGCCATTGTGGATGCCAAAGGCGAACTAAAAGTATCAGAGGAAGATGCCAAGAACCTCTGTTTTTGGTCCAGGGTTTGCCTTCATAACATGGCAAAGTTAGCCAAAGAGGCTACAACAATAAGACGTGTCCTGGAATCTTTGTTCCGCTACTTTGATAACGAAAACTTGTGGTCTCTTGAACATGGGCTTGCCATCTCAGCTTTCCCAGAGGCATTGTTTCACCAATTACTCCCAGCTATGGTTCATCCAGACCATGAAACACGTGTTGGAGCTCATCGCATCTTCTCTGTTGTTCTTGTGCCATCTTCAGTTTCGCCTAATCTATTCTCATCTAATACCGAGACAAAAAAGGCCTTTGATCTTCACAGGACACTCTCAAGAACTGTCTCTGTGTTTTCTTCTTCAGCTGCCCTTTTTGAGAAGCTGAGGAGAGAGAAAATTTCCTCACGGGAAAGTATTTGTGAAGAAGATGAAAACATTGTTCCGGAGGGGGAAGAAAGAGACACCAATAATGGGTTTCTGAGCAGATTGAAGTCATCTTATAGTCGGACATATAGTATGAAAAGCTTGCCTGCACCTTCAACACCAAATGAAAATTCCATGAGCAATACTAAGGAACTTGAAAAGGTCACAAAGCTACTAACTTGTGTAGATACAGTAGCAGTCTCAGATCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

465

Amino Acids

52.53

Weight (kDa)

7.87

Isoelectric Point (pI)

44.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EFR3_ARM PF21052 69 - 158 1e-17 EFR3, armadillo repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 8
AccIII TCCGGA 1 cut(s) 1204
AciI CCGC 4 cut(s) 8, 48, 211, 865
AclWI GGATC 2 cut(s) 273, 393
AcsI RAATTY 3 cut(s) 271, 1158, 1315
AcuI CTGAAG 3 cut(s) 648, 1011, 1107
AcvI CACGTG 1 cut(s) 983
AfaI GTAC 1 cut(s) 110
AfiI CCNNNNNNNGG 5 cut(s) 47, 90, 105, 958, 1233
AflIII ACRYGT 4 cut(s) 468, 844, 980, 982
AgsI TTSAA 6 cut(s) 579, 662, 899, 1252, 1302, 1343
AjiI CACGTC 1 cut(s) 845
AjnI CCWGG 5 cut(s) 83, 144, 520, 788, 849
AjuI GAANNNNNNNTTGG 4 cut(s) 969, 1001, 1300, 1332
AloI GAACNNNNNNTCC 2 cut(s) 845, 877
Alw21I GWGCWC 1 cut(s) 994
Alw26I GTCTC 7 cut(s) 30, 59, 897, 1055, 1111, 1215, 1390
AlwI GGATC 2 cut(s) 273, 393
AlwNI CAGNNNCTG 1 cut(s) 57
Ama87I CYCGRG 1 cut(s) 71
Aor13HI TCCGGA 1 cut(s) 1204
AoxI GGCC 1 cut(s) 1071
ApeKI GCWGC 5 cut(s) 76, 94, 194, 557, 1127
ApoI RAATTY 3 cut(s) 271, 1158, 1315
AspLEI GCGC 1 cut(s) 8
AspS9I GGNCC 3 cut(s) 87, 155, 786
AsuC2I CCSGG 3 cut(s) 72, 73, 91
AsuHPI GGTGA 1 cut(s) 932
AvaI CYCGRG 1 cut(s) 71
AvaII GGWCC 3 cut(s) 87, 155, 786
BanII GRGCYC 1 cut(s) 994
BbrPI CACGTG 1 cut(s) 983
Bbv12I GWGCWC 1 cut(s) 994
BbvCI CCTCAGC 1 cut(s) 1145
BbvI GCAGC 5 cut(s) 63, 106, 181, 544, 1114
BccI CCATC 3 cut(s) 452, 920, 1030
BcgI CGANNNNNNTGC 2 cut(s) 340, 374
BciT130I CCWGG 5 cut(s) 85, 146, 522, 790, 851
BcnI CCSGG 3 cut(s) 72, 73, 91
BcoDI GTCTC 7 cut(s) 30, 59, 897, 1055, 1111, 1215, 1390
BglI GCCNNNNNGGC 1 cut(s) 827
BglII AGATCT 1 cut(s) 1390
BisI GCNGC 6 cut(s) 49, 77, 95, 195, 558, 1128
BlpI GCTNAGC 1 cut(s) 362
BlsI GCNGC 6 cut(s) 50, 78, 96, 196, 559, 1129
Bme1390I CCNGG 8 cut(s) 72, 73, 85, 91, 146, 522, 790, 851
Bme18I GGWCC 3 cut(s) 87, 155, 786
BmeT110I CYCGRG 1 cut(s) 71
BmgBI CACGTC 1 cut(s) 845
BmgT120I GGNCC 3 cut(s) 87, 155, 786
BmiI GGNNCC 1 cut(s) 89
BmrFI CCNGG 8 cut(s) 72, 73, 85, 91, 146, 522, 790, 851
BmsI GCATC 4 cut(s) 410, 721, 754, 1008
Bpu10I CCTNAGC 1 cut(s) 1145
Bpu1102I GCTNAGC 1 cut(s) 362
BpuEI CTTGAG 3 cut(s) 257, 498, 1081
BpuMI CCSGG 3 cut(s) 72, 73, 91
BsaAI YACGTR 1 cut(s) 983
BsaI GGTCTC 1 cut(s) 897
BsaJI CCNNGG 5 cut(s) 70, 71, 521, 713, 789
BsaWI WCCGGW 1 cut(s) 1204
Bsc4I CCNNNNNNNGG 5 cut(s) 47, 90, 105, 958, 1233
BseAI TCCGGA 1 cut(s) 1204
BseBI CCWGG 5 cut(s) 85, 146, 522, 790, 851
BseDI CCNNGG 5 cut(s) 70, 71, 521, 713, 789
BseGI GGATG 4 cut(s) 425, 463, 736, 964
BseLI CCNNNNNNNGG 5 cut(s) 47, 90, 105, 958, 1233
BseMII CTCAG 4 cut(s) 376, 930, 1136, 1232
BseRI GAGGAG 1 cut(s) 1162
BseXI GCAGC 5 cut(s) 63, 106, 181, 544, 1114
BseYI CCCAGC 1 cut(s) 952
BsgI GTGCAG 3 cut(s) 543, 671, 1278
Bsh1236I CGCG 1 cut(s) 8
BshFI GGCC 1 cut(s) 1073
BsiHKAI GWGCWC 1 cut(s) 994
BsiHKCI CYCGRG 1 cut(s) 71
BsiSI CCGG 3 cut(s) 72, 91, 1205
BslFI GGGAC 1 cut(s) 73
BslI CCNNNNNNNGG 5 cut(s) 47, 90, 105, 958, 1233
BsmAI GTCTC 7 cut(s) 30, 59, 897, 1055, 1111, 1215, 1390
BsmFI GGGAC 1 cut(s) 73
BsnI GGCC 1 cut(s) 1073
Bso31I GGTCTC 1 cut(s) 897
BsoBI CYCGRG 1 cut(s) 71
Bsp1286I GDGCHC 1 cut(s) 994
Bsp13I TCCGGA 1 cut(s) 1204
Bsp143I GATC 6 cut(s) 163, 222, 278, 385, 1078, 1390
Bsp1720I GCTNAGC 1 cut(s) 362
BspACI CCGC 4 cut(s) 8, 48, 211, 865
BspANI GGCC 1 cut(s) 1073
BspCNI CTCAG 4 cut(s) 375, 929, 1137, 1233
BspEI TCCGGA 1 cut(s) 1204
BspFNI CGCG 1 cut(s) 8
BspLI GGNNCC 1 cut(s) 89
BspPI GGATC 2 cut(s) 273, 393
BspTNI GGTCTC 1 cut(s) 897
BssECI CCNNGG 5 cut(s) 70, 71, 521, 713, 789
BssMI GATC 6 cut(s) 163, 222, 278, 385, 1078, 1390
BssT1I CCWWGG 1 cut(s) 713
Bst2UI CCWGG 5 cut(s) 85, 146, 522, 790, 851
Bst4CI ACNGT 2 cut(s) 1105, 1378
BstBAI YACGTR 1 cut(s) 983
BstC8I GCNNGC 8 cut(s) 42, 46, 81, 359, 567, 909, 1289, 1293
BstDEI CTNAG 7 cut(s) 362, 478, 916, 1145, 1241, 1332, 1387
BstF5I GGATG 4 cut(s) 425, 463, 736, 964
BstFNI CGCG 1 cut(s) 8
BstHHI GCGC 1 cut(s) 8
BstKTI GATC 6 cut(s) 166, 225, 281, 388, 1081, 1393
BstMAI GTCTC 7 cut(s) 30, 59, 897, 1055, 1111, 1215, 1390
BstMBI GATC 6 cut(s) 163, 222, 278, 385, 1078, 1390
BstMWI GCNNNNNNNGC 5 cut(s) 363, 566, 818, 827, 917
BstNI CCWGG 5 cut(s) 85, 146, 522, 790, 851
BstNSI RCATGY 1 cut(s) 472
BstSCI CCNGG 8 cut(s) 70, 71, 83, 89, 144, 520, 788, 849
BstUI CGCG 1 cut(s) 8
BstV1I GCAGC 5 cut(s) 63, 106, 181, 544, 1114
BstX2I RGATCY 1 cut(s) 1390
BstYI RGATCY 1 cut(s) 1390
BsuRI GGCC 1 cut(s) 1073
BtgZI GCGATG 2 cut(s) 106, 980
BtrI CACGTC 1 cut(s) 845
BtsCI GGATG 4 cut(s) 425, 463, 736, 964
BtsI GCAGTG 2 cut(s) 560, 597
BtsIMutI CAGTG 3 cut(s) 560, 597, 650
Cac8I GCNNGC 8 cut(s) 42, 46, 81, 359, 567, 909, 1289, 1293
CaiI CAGNNNCTG 1 cut(s) 57
CfoI GCGC 1 cut(s) 8
Cfr13I GGNCC 3 cut(s) 87, 155, 786
Cfr9I CCCGGG 1 cut(s) 71
Csp6I GTAC 1 cut(s) 109
CspCI CAANNNNNGTGG 2 cut(s) 271, 306
CviAII CATG 7 cut(s) 295, 469, 677, 808, 902, 974, 1321
CviQI GTAC 1 cut(s) 109
DdeI CTNAG 7 cut(s) 362, 478, 916, 1145, 1241, 1332, 1387
DpnI GATC 6 cut(s) 165, 224, 280, 387, 1080, 1392
DpnII GATC 6 cut(s) 163, 222, 278, 385, 1078, 1390
Ecl136II GAGCTC 1 cut(s) 992
Eco130I CCWWGG 1 cut(s) 713
Eco147I AGGCCT 1 cut(s) 1073
Eco24I GRGCYC 1 cut(s) 994
Eco31I GGTCTC 1 cut(s) 897
Eco47I GGWCC 3 cut(s) 87, 155, 786
Eco53kI GAGCTC 1 cut(s) 992
Eco57I CTGAAG 3 cut(s) 648, 1011, 1107
Eco72I CACGTG 1 cut(s) 983
Eco88I CYCGRG 1 cut(s) 71
EcoICRI GAGCTC 1 cut(s) 992
EcoO109I RGGNCCY 1 cut(s) 87
EcoRII CCWGG 5 cut(s) 83, 144, 520, 788, 849
EcoT14I CCWWGG 1 cut(s) 713
EcoT22I ATGCAT 1 cut(s) 375
EcoT38I GRGCYC 1 cut(s) 994
ErhI CCWWGG 1 cut(s) 713
FaeI CATG 7 cut(s) 298, 472, 680, 811, 905, 977, 1324
FaqI GGGAC 1 cut(s) 73
FatI CATG 7 cut(s) 294, 468, 676, 807, 901, 973, 1320
Fnu4HI GCNGC 6 cut(s) 49, 77, 95, 195, 558, 1128
FokI GGATG 4 cut(s) 432, 470, 743, 951
FriOI GRGCYC 1 cut(s) 994
Fsp4HI GCNGC 6 cut(s) 49, 77, 95, 195, 558, 1128
GlaI GCGC 1 cut(s) 7
GluI GCNGC 6 cut(s) 49, 77, 95, 195, 558, 1128
GsaI CCCAGC 1 cut(s) 956
HaeIII GGCC 1 cut(s) 1073
HapII CCGG 3 cut(s) 72, 91, 1205
HhaI GCGC 1 cut(s) 8
Hin1II CATG 7 cut(s) 298, 472, 680, 811, 905, 977, 1324
Hin6I GCGC 1 cut(s) 6
HinP1I GCGC 1 cut(s) 6
HindIII AAGCTT 1 cut(s) 1285
HinfI GANTC 6 cut(s) 214, 427, 640, 699, 706, 854
HpaII CCGG 3 cut(s) 72, 91, 1205
HphI GGTGA 1 cut(s) 932
Hpy188I TCNGA 9 cut(s) 168, 495, 507, 698, 757, 1242, 1269, 1390, 1397
Hpy188III TCNNGA 9 cut(s) 103, 236, 383, 515, 703, 896, 968, 1098, 1205
HpyAV CCTTC 6 cut(s) 146, 389, 476, 623, 809, 1308
HpyCH4III ACNGT 2 cut(s) 1105, 1378
HpyCH4IV ACGT 3 cut(s) 550, 844, 982
HpyCH4V TGCA 9 cut(s) 300, 327, 373, 409, 560, 565, 590, 652, 1295
HpyF10VI GCNNNNNNNGC 5 cut(s) 363, 566, 818, 827, 917
HpyF3I CTNAG 7 cut(s) 362, 478, 916, 1145, 1241, 1332, 1387
HpySE526I ACGT 3 cut(s) 550, 844, 982
Hsp92II CATG 7 cut(s) 298, 472, 680, 811, 905, 977, 1324
HspAI GCGC 1 cut(s) 6
Kpn2I TCCGGA 1 cut(s) 1204
Kzo9I GATC 6 cut(s) 163, 222, 278, 385, 1078, 1390
LmnI GCTCC 2 cut(s) 331, 989
Lsp1109I GCAGC 5 cut(s) 63, 106, 181, 544, 1114
LweI GCATC 4 cut(s) 410, 721, 754, 1008
MaeII ACGT 3 cut(s) 550, 844, 982
MaeIII GTNAC 2 cut(s) 248, 1348
MalI GATC 6 cut(s) 165, 224, 280, 387, 1080, 1392
MboI GATC 6 cut(s) 163, 222, 278, 385, 1078, 1390
MflI RGATCY 1 cut(s) 1390
MhlI GDGCHC 1 cut(s) 994
MlyI GAGTC 1 cut(s) 421
MmeI TCCRAC 2 cut(s) 967, 1247
Mph1103I ATGCAT 1 cut(s) 375
MroI TCCGGA 1 cut(s) 1204
MseI TTAA 2 cut(s) 474, 618
MspA1I CMGCKG 1 cut(s) 1127
MspI CCGG 3 cut(s) 72, 91, 1205
MspR9I CCNGG 8 cut(s) 72, 73, 85, 91, 146, 522, 790, 851
MvaI CCWGG 5 cut(s) 85, 146, 522, 790, 851
MvnI CGCG 1 cut(s) 8
MwoI GCNNNNNNNGC 5 cut(s) 363, 566, 818, 827, 917
NciI CCSGG 3 cut(s) 72, 73, 91
NdeII GATC 6 cut(s) 163, 222, 278, 385, 1078, 1390
NlaIII CATG 7 cut(s) 298, 472, 680, 811, 905, 977, 1324
NlaIV GGNNCC 1 cut(s) 89
NmuCI GTSAC 1 cut(s) 1348
NsiI ATGCAT 1 cut(s) 375
NspI RCATGY 1 cut(s) 472
PceI AGGCCT 1 cut(s) 1073
PciI ACATGT 1 cut(s) 468
PfeI GAWTC 5 cut(s) 214, 640, 699, 706, 854
PfoI TCCNGGA 1 cut(s) 849
PkrI GCNGC 6 cut(s) 50, 78, 96, 196, 559, 1129
PleI GAGTC 1 cut(s) 421
PmaCI CACGTG 1 cut(s) 983
PmlI CACGTG 1 cut(s) 983
PpsI GAGTC 1 cut(s) 421
Ppu21I YACGTR 1 cut(s) 983
PpuMI RGGWCCY 1 cut(s) 87
PscI ACATGT 1 cut(s) 468
Psp124BI GAGCTC 1 cut(s) 994
Psp5II RGGWCCY 1 cut(s) 87
Psp6I CCWGG 5 cut(s) 83, 144, 520, 788, 849
PspCI CACGTG 1 cut(s) 983
PspFI CCCAGC 1 cut(s) 952
PspGI CCWGG 5 cut(s) 83, 144, 520, 788, 849
PspN4I GGNNCC 1 cut(s) 89
PspPI GGNCC 3 cut(s) 87, 155, 786
PspPPI RGGWCCY 1 cut(s) 87
PstNI CAGNNNCTG 1 cut(s) 57
PsuI RGATCY 1 cut(s) 1390
PvuII CAGCTG 1 cut(s) 1127
RsaI GTAC 1 cut(s) 110
RsaNI GTAC 1 cut(s) 109
SacI GAGCTC 1 cut(s) 994
SaqAI TTAA 2 cut(s) 474, 618
SatI GCNGC 6 cut(s) 49, 77, 95, 195, 558, 1128
Sau3AI GATC 6 cut(s) 163, 222, 278, 385, 1078, 1390
Sau96I GGNCC 3 cut(s) 87, 155, 786
SchI GAGTC 1 cut(s) 421
ScrFI CCNGG 8 cut(s) 72, 73, 85, 91, 146, 522, 790, 851
SduI GDGCHC 1 cut(s) 994
SfaNI GCATC 4 cut(s) 410, 721, 754, 1008
SinI GGWCC 3 cut(s) 87, 155, 786
SmaI CCCGGG 1 cut(s) 73
SmlI CTYRAG 3 cut(s) 236, 513, 1096
SmoI CTYRAG 3 cut(s) 236, 513, 1096
SseBI AGGCCT 1 cut(s) 1073
SsiI CCGC 4 cut(s) 8, 48, 211, 865
SstI GAGCTC 1 cut(s) 994
StuI AGGCCT 1 cut(s) 1073
StyD4I CCNGG 8 cut(s) 70, 71, 83, 89, 144, 520, 788, 849
StyI CCWWGG 1 cut(s) 713
TaaI ACNGT 2 cut(s) 1105, 1378
TaiI ACGT 3 cut(s) 553, 847, 985
TaqI TCGA 1 cut(s) 277
TauI GCSGC 1 cut(s) 51
TfiI GAWTC 5 cut(s) 214, 640, 699, 706, 854
Tru1I TTAA 2 cut(s) 474, 618
Tru9I TTAA 2 cut(s) 474, 618
TscAI CASTG 3 cut(s) 567, 597, 650
TseFI GTSAC 1 cut(s) 1348
TseI GCWGC 5 cut(s) 76, 94, 194, 557, 1127
Tsp45I GTSAC 1 cut(s) 1348
TspDTI ATGAA 8 cut(s) 417, 546, 791, 953, 990, 1206, 1295, 1326
TspMI CCCGGG 1 cut(s) 71
TspRI CASTG 3 cut(s) 567, 597, 650
VpaK11BI GGWCC 3 cut(s) 87, 155, 786
XapI RAATTY 3 cut(s) 271, 1158, 1315
XceI RCATGY 1 cut(s) 472
XmaI CCCGGG 1 cut(s) 71
Zsp2I ATGCAT 1 cut(s) 375
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.