Rh1DG017900

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0002545
Physical Location & Seq
Forward (+)
2876439 .. 2891115
14677 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG017900.1

Sequence Viewer

Length: 582 bp
ATGGATTCTTTTCTGTACTACCTTTATTCGGTTGCGATGTTGATCCTGGTTGCGGCTTTTGTAGATTTCACGTCGATCTCTTGGGAATCTAAGAATGACGAGTCTGGGGCCTTCATAGTGACTCAAGCTAATCCATATCAAGCCATCGCCGAGATTTTTCCTCGCTCTCCGGATGAAGAACCAAATGACCGCAAGATCAGTAAACTGTGTGAATATGCCTCAAAAACTCCTCTCCGTATTCCAAAGATCACATCTACTCTGGAGCAAAGATGTTACAAGGACTTGAGAACTGAGAACTTCCACTCTGTGAAAGTGGTCATGTGTATCTACCGGAAGTTGTTGATTTCTTGTAAGGATCAAATGCCATTGTTTGCAAGTAGTCCACTTAGCATTGCTCAAATTCTGCTTGATCAAACACGACATGATGAAATACCTCAAGAAATGAGGGAGGATGGGACAGCAATGAATTTGCGGGCAGCTGGGCTTCAAGCTCTTTCATCAATGCATCTTGATCACAAGAATGTTCTTAAAAACCCAAATATGCAGCTGGACATTGTTGATGTTGCCACCTCGCTTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

193

Amino Acids

21.89

Weight (kDa)

5.57

Isoelectric Point (pI)

38.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EFR3_ARM PF21052 77 - 141 1.2e-10 EFR3, armadillo repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 169
AciI CCGC 3 cut(s) 53, 190, 472
AclWI GGATC 2 cut(s) 37, 363
AcsI RAATTY 2 cut(s) 399, 466
AdeI CACNNNGTG 1 cut(s) 307
AfaI GTAC 1 cut(s) 17
AfiI CCNNNNNNNGG 2 cut(s) 28, 52
AgsI TTSAA 1 cut(s) 488
AjiI CACGTC 1 cut(s) 72
AjnI CCWGG 1 cut(s) 45
AluBI AGCT 4 cut(s) 128, 479, 491, 547
AluI AGCT 4 cut(s) 128, 479, 491, 547
AlwI GGATC 2 cut(s) 37, 363
Aor13HI TCCGGA 1 cut(s) 169
AoxI GGCC 1 cut(s) 108
ApeKI GCWGC 2 cut(s) 476, 544
ApoI RAATTY 2 cut(s) 399, 466
AspS9I GGNCC 1 cut(s) 108
BbvI GCAGC 2 cut(s) 488, 556
BccI CCATC 2 cut(s) 152, 446
BciT130I CCWGG 1 cut(s) 47
BclI TGATCA 2 cut(s) 409, 511
BisI GCNGC 3 cut(s) 54, 477, 545
BlsI GCNGC 3 cut(s) 55, 478, 546
Bme1390I CCNGG 1 cut(s) 47
BmgBI CACGTC 1 cut(s) 72
BmgT120I GGNCC 1 cut(s) 108
BmiI GGNNCC 1 cut(s) 109
BmrFI CCNGG 1 cut(s) 47
BmsI GCATC 1 cut(s) 514
BpmI CTGGAG 1 cut(s) 281
BpuEI CTTGAG 3 cut(s) 108, 304, 420
BsaBI GATNNNNATC 1 cut(s) 41
BsaWI WCCGGW 2 cut(s) 169, 330
Bsc4I CCNNNNNNNGG 2 cut(s) 28, 52
Bse3DI GCAATG 2 cut(s) 390, 468
Bse8I GATNNNNATC 1 cut(s) 41
BseAI TCCGGA 1 cut(s) 169
BseBI CCWGG 1 cut(s) 47
BseGI GGATG 2 cut(s) 178, 457
BseJI GATNNNNATC 1 cut(s) 41
BseLI CCNNNNNNNGG 2 cut(s) 28, 52
BseMI GCAATG 2 cut(s) 390, 468
BseMII CTCAG 1 cut(s) 282
BseRI GAGGAG 1 cut(s) 219
BseXI GCAGC 2 cut(s) 488, 556
BseYI CCCAGC 1 cut(s) 479
BshFI GGCC 1 cut(s) 110
BsiSI CCGG 2 cut(s) 170, 331
BslFI GGGAC 1 cut(s) 469
BslI CCNNNNNNNGG 2 cut(s) 28, 52
BsmFI GGGAC 1 cut(s) 469
BsnI GGCC 1 cut(s) 110
Bsp13I TCCGGA 1 cut(s) 169
Bsp143I GATC 7 cut(s) 42, 75, 195, 246, 355, 409, 511
BspACI CCGC 3 cut(s) 53, 190, 472
BspANI GGCC 1 cut(s) 110
BspCNI CTCAG 1 cut(s) 283
BspEI TCCGGA 1 cut(s) 169
BspLI GGNNCC 1 cut(s) 109
BspPI GGATC 2 cut(s) 37, 363
BsrDI GCAATG 2 cut(s) 390, 468
BssMI GATC 7 cut(s) 42, 75, 195, 246, 355, 409, 511
Bst2UI CCWGG 1 cut(s) 47
Bst4CI ACNGT 1 cut(s) 207
BstC8I GCNNGC 2 cut(s) 474, 576
BstDEI CTNAG 3 cut(s) 90, 291, 386
BstF5I GGATG 2 cut(s) 178, 457
BstKTI GATC 7 cut(s) 45, 78, 198, 249, 358, 412, 514
BstMBI GATC 7 cut(s) 42, 75, 195, 246, 355, 409, 511
BstNI CCWGG 1 cut(s) 47
BstSCI CCNGG 1 cut(s) 45
BstV1I GCAGC 2 cut(s) 488, 556
BsuRI GGCC 1 cut(s) 110
BtgZI GCGATG 2 cut(s) 50, 130
BtrI CACGTC 1 cut(s) 72
BtsCI GGATG 2 cut(s) 178, 457
Cac8I GCNNGC 2 cut(s) 474, 576
Cfr13I GGNCC 1 cut(s) 108
Csp6I GTAC 1 cut(s) 16
CspCI CAANNNNNGTGG 1 cut(s) 556
CviAII CATG 2 cut(s) 319, 422
CviJI RGCY 8 cut(s) 56, 110, 128, 143, 479, 484, 491, 547
CviKI_1 RGCY 8 cut(s) 56, 110, 128, 143, 479, 484, 491, 547
CviQI GTAC 1 cut(s) 16
DdeI CTNAG 3 cut(s) 90, 291, 386
DpnI GATC 7 cut(s) 44, 77, 197, 248, 357, 411, 513
DpnII GATC 7 cut(s) 42, 75, 195, 246, 355, 409, 511
DraIII CACNNNGTG 1 cut(s) 307
EcoO109I RGGNCCY 1 cut(s) 108
EcoRII CCWGG 1 cut(s) 45
EcoT22I ATGCAT 1 cut(s) 507
FaeI CATG 2 cut(s) 322, 425
FaiI YATR 6 cut(s) 116, 136, 216, 320, 423, 542
FaqI GGGAC 1 cut(s) 469
FatI CATG 2 cut(s) 318, 421
FauI CCCGC 1 cut(s) 465
FbaI TGATCA 2 cut(s) 409, 511
Fnu4HI GCNGC 3 cut(s) 54, 477, 545
FokI GGATG 2 cut(s) 185, 464
Fsp4HI GCNGC 3 cut(s) 54, 477, 545
GluI GCNGC 3 cut(s) 54, 477, 545
GsaI CCCAGC 1 cut(s) 483
GsuI CTGGAG 1 cut(s) 281
HaeIII GGCC 1 cut(s) 110
HapII CCGG 2 cut(s) 170, 331
Hin1II CATG 2 cut(s) 322, 425
HinfI GANTC 4 cut(s) 5, 86, 101, 121
HpaII CCGG 2 cut(s) 170, 331
Hpy166II GTNNAC 2 cut(s) 203, 383
Hpy188III TCNNGA 4 cut(s) 170, 260, 437, 509
Hpy8I GTNNAC 2 cut(s) 203, 383
Hpy99I CGWCG 1 cut(s) 76
HpyAV CCTTC 1 cut(s) 121
HpyCH4III ACNGT 1 cut(s) 207
HpyCH4IV ACGT 1 cut(s) 71
HpyCH4V TGCA 3 cut(s) 374, 505, 544
HpyF3I CTNAG 3 cut(s) 90, 291, 386
HpySE526I ACGT 1 cut(s) 71
Hsp92II CATG 2 cut(s) 322, 425
Kpn2I TCCGGA 1 cut(s) 169
Ksp22I TGATCA 2 cut(s) 409, 511
Kzo9I GATC 7 cut(s) 42, 75, 195, 246, 355, 409, 511
LmnI GCTCC 1 cut(s) 262
LpnPI CCDG 8 cut(s) 32, 59, 90, 183, 245, 344, 465, 533
Lsp1109I GCAGC 2 cut(s) 488, 556
LweI GCATC 1 cut(s) 514
MaeII ACGT 1 cut(s) 71
MaeIII GTNAC 2 cut(s) 118, 272
MalI GATC 7 cut(s) 44, 77, 197, 248, 357, 411, 513
MboI GATC 7 cut(s) 42, 75, 195, 246, 355, 409, 511
MboII GAAGA 1 cut(s) 188
MluCI AATT 2 cut(s) 399, 466
MlyI GAGTC 2 cut(s) 110, 115
MnlI CCTC 7 cut(s) 171, 229, 240, 438, 442, 444, 580
Mph1103I ATGCAT 1 cut(s) 507
MroI TCCGGA 1 cut(s) 169
MseI TTAA 1 cut(s) 528
MslI CAYNNNNRTG 1 cut(s) 519
MspA1I CMGCKG 2 cut(s) 479, 547
MspI CCGG 2 cut(s) 170, 331
MspR9I CCNGG 1 cut(s) 47
MvaI CCWGG 1 cut(s) 47
NdeII GATC 7 cut(s) 42, 75, 195, 246, 355, 409, 511
NlaIII CATG 2 cut(s) 322, 425
NlaIV GGNNCC 1 cut(s) 109
NmeAIII GCCGAG 1 cut(s) 175
NmuCI GTSAC 1 cut(s) 118
NsiI ATGCAT 1 cut(s) 507
PfeI GAWTC 2 cut(s) 5, 86
PkrI GCNGC 3 cut(s) 55, 478, 546
PleI GAGTC 2 cut(s) 109, 115
PpsI GAGTC 2 cut(s) 109, 115
Psp6I CCWGG 1 cut(s) 45
PspFI CCCAGC 1 cut(s) 479
PspGI CCWGG 1 cut(s) 45
PspN4I GGNNCC 1 cut(s) 109
PspPI GGNCC 1 cut(s) 108
PvuII CAGCTG 2 cut(s) 479, 547
RsaI GTAC 1 cut(s) 17
RsaNI GTAC 1 cut(s) 16
RseI CAYNNNNRTG 1 cut(s) 519
SaqAI TTAA 1 cut(s) 528
SatI GCNGC 3 cut(s) 54, 477, 545
Sau3AI GATC 7 cut(s) 42, 75, 195, 246, 355, 409, 511
Sau96I GGNCC 1 cut(s) 108
SchI GAGTC 2 cut(s) 110, 115
ScrFI CCNGG 1 cut(s) 47
SetI ASST 8 cut(s) 24, 74, 130, 436, 481, 493, 549, 572
SfaNI GCATC 1 cut(s) 514
SmiMI CAYNNNNRTG 1 cut(s) 519
SmlI CTYRAG 3 cut(s) 123, 283, 435
SmoI CTYRAG 3 cut(s) 123, 283, 435
Sse9I AATT 2 cut(s) 399, 466
SsiI CCGC 3 cut(s) 53, 190, 472
StyD4I CCNGG 1 cut(s) 45
TaaI ACNGT 1 cut(s) 207
TaiI ACGT 1 cut(s) 74
TaqI TCGA 1 cut(s) 74
TasI AATT 2 cut(s) 399, 466
TatI WGTACW 1 cut(s) 15
TauI GCSGC 1 cut(s) 56
TfiI GAWTC 2 cut(s) 5, 86
Tru1I TTAA 1 cut(s) 528
Tru9I TTAA 1 cut(s) 528
TseFI GTSAC 1 cut(s) 118
TseI GCWGC 2 cut(s) 476, 544
Tsp45I GTSAC 1 cut(s) 118
TspDTI ATGAA 5 cut(s) 103, 189, 441, 479, 486
TspGWI ACGGA 1 cut(s) 224
XapI RAATTY 2 cut(s) 399, 466
Zsp2I ATGCAT 1 cut(s) 507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.