Rh4BG137900

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0002545
Physical Location & Seq
Forward (+)
24567306 .. 24574238
6933 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG137900.1

Sequence Viewer

Length: 927 bp
ATGGGGGTTGTATCGAGGCGAGTTGTGCCCGCCTGCGGTAGCCTCTGTTTCTTCTGTCCCTCACTGCGTGCAAGGTCGAGGCAACCAGTCAAACGCTACAAGAAGTTGCTCGCTGAGATCTTCCCCCGTAATCAGGATGCTGAACCCAATGACAGAAAGATTGGGAAACTTTGTGAATATGCTCTGAAGAACCCATTGCGAATTCCCAAGATTACTGAGCATCTAGAGCAAAAATGTTACAAGGATTTGCGAAACGAACAGTTTGGATCTGTTAAAGTCATACTGTGCATTTATAGGAAGTTACTATCTTCCTGTAAAGTGCAGATGCCACTATTTGCAAGTAGTCTACTAGGAATTGTTCGGATTCTCTTAGAACAAACCCGACATGATGAAATGCGGATTCTGGGTTGCAATACACTTGTCGACTTTATAAATAGCCAGATTGATAGTACACACATGTTCAACCTAGAAGGCCTTATTCCTAAACTCTGTGAATTGGCTCAAGAAATGGGAGATGATGAAAGAGCTCTACGTCTACGTTCAGCTGGATTGCAATCACTGGCTTTTATGATCATATCAGTGACTATGGAAAATTATACCAATATGCATACTAAACCTGGGAGTGTGAAAGAAGACGGACAATTTTCTGAATCTCAGCACCAGTGGGTTCAAAAAGCGCTAAAAGCAGAAGTTCATGATTCATCTTTTCCAGATGTTAGTCAGAAGGTTGGAGATGCAGGGCCCATACTTGATATGATGGCTGTGGTGCTGGAGAATATCCCATCTAGTACTATTGTAGCTAGGGCGACAGTCTCTGCTGTTTATCTAACTGCAAAGATGGTCTCTTCTGTTCCAAACATATCCTATCACAAAAAGGCAAGGGAAACTTTTTCAGATTTCACTTTTTTCATGTTCAAATTTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

308

Amino Acids

34.88

Weight (kDa)

9.08

Isoelectric Point (pI)

47.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EFR3_ARM PF21052 65 - 151 8e-19 EFR3, armadillo repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 431
AccI GTMKAC 3 cut(s) 346, 423, 535
AciI CCGC 3 cut(s) 30, 36, 397
AclWI GGATC 1 cut(s) 274
AcsI RAATTY 2 cut(s) 201, 917
AcuI CTGAAG 1 cut(s) 206
AdeI CACNNNGTG 1 cut(s) 68
AfaI GTAC 2 cut(s) 451, 790
AfeI AGCGCT 1 cut(s) 678
AfiI CCNNNNNNNGG 2 cut(s) 35, 133
AflIII ACRYGT 1 cut(s) 456
AgsI TTSAA 3 cut(s) 463, 671, 916
AjnI CCWGG 1 cut(s) 616
AluBI AGCT 3 cut(s) 527, 545, 800
AluI AGCT 3 cut(s) 527, 545, 800
Alw21I GWGCWC 1 cut(s) 529
Alw26I GTCTC 2 cut(s) 817, 847
AlwI GGATC 1 cut(s) 274
AlwNI CAGNNNCTG 1 cut(s) 815
Aor51HI AGCGCT 1 cut(s) 678
AoxI GGCC 2 cut(s) 472, 740
ApaI GGGCCC 1 cut(s) 744
ApoI RAATTY 2 cut(s) 201, 917
AspLEI GCGC 1 cut(s) 679
AspS9I GGNCC 2 cut(s) 740, 741
BaeGI GKGCMC 2 cut(s) 30, 744
BanII GRGCYC 2 cut(s) 529, 744
BbsI GAAGAC 1 cut(s) 639
Bbv12I GWGCWC 1 cut(s) 529
BccI CCATC 3 cut(s) 751, 790, 832
BciT130I CCWGG 1 cut(s) 618
BclI TGATCA 1 cut(s) 570
BcoDI GTCTC 2 cut(s) 817, 847
BfaI CTAG 5 cut(s) 224, 350, 467, 786, 801
BfoI RGCGCY 1 cut(s) 680
BglII AGATCT 1 cut(s) 117
BmcAI AGTACT 1 cut(s) 790
Bme1390I CCNGG 1 cut(s) 618
BmgT120I GGNCC 2 cut(s) 740, 741
BmiI GGNNCC 1 cut(s) 742
BmrFI CCNGG 1 cut(s) 618
BmsI GCATC 4 cut(s) 127, 229, 315, 724
BpiI GAAGAC 1 cut(s) 639
BpmI CTGGAG 1 cut(s) 791
BpuEI CTTGAG 1 cut(s) 486
BsaBI GATNNNNATC 1 cut(s) 553
BsaI GGTCTC 1 cut(s) 847
BsaJI CCNNGG 1 cut(s) 617
Bsc4I CCNNNNNNNGG 2 cut(s) 35, 133
Bse1I ACTGG 3 cut(s) 86, 564, 661
Bse3DI GCAATG 1 cut(s) 194
Bse8I GATNNNNATC 1 cut(s) 553
BseBI CCWGG 1 cut(s) 618
BseDI CCNNGG 1 cut(s) 617
BseGI GGATG 1 cut(s) 142
BseJI GATNNNNATC 1 cut(s) 553
BseLI CCNNNNNNNGG 2 cut(s) 35, 133
BseMI GCAATG 1 cut(s) 194
BseMII CTCAG 3 cut(s) 105, 207, 668
BseNI ACTGG 3 cut(s) 86, 564, 661
BseSI GKGCMC 2 cut(s) 30, 744
BsgI GTGCAG 1 cut(s) 341
BshFI GGCC 2 cut(s) 474, 742
BsiHKAI GWGCWC 1 cut(s) 529
BslFI GGGAC 1 cut(s) 42
BslI CCNNNNNNNGG 2 cut(s) 35, 133
BsmAI GTCTC 2 cut(s) 817, 847
BsmFI GGGAC 1 cut(s) 42
BsnI GGCC 2 cut(s) 474, 742
Bso31I GGTCTC 1 cut(s) 847
Bsp120I GGGCCC 1 cut(s) 740
Bsp1286I GDGCHC 3 cut(s) 30, 529, 744
Bsp143I GATC 3 cut(s) 117, 266, 570
BspACI CCGC 3 cut(s) 30, 36, 397
BspANI GGCC 2 cut(s) 474, 742
BspCNI CTCAG 3 cut(s) 106, 208, 667
BspHI TCATGA 1 cut(s) 694
BspLI GGNNCC 1 cut(s) 742
BspPI GGATC 1 cut(s) 274
BspTNI GGTCTC 1 cut(s) 847
BsrDI GCAATG 1 cut(s) 194
BsrI ACTGG 3 cut(s) 86, 564, 661
BssECI CCNNGG 1 cut(s) 617
BssMI GATC 3 cut(s) 117, 266, 570
Bst2UI CCWGG 1 cut(s) 618
Bst4CI ACNGT 3 cut(s) 261, 285, 811
Bst6I CTCTTC 1 cut(s) 850
BstC8I GCNNGC 4 cut(s) 30, 34, 69, 111
BstDEI CTNAG 4 cut(s) 114, 216, 370, 654
BstF5I GGATG 1 cut(s) 142
BstH2I RGCGCY 1 cut(s) 680
BstHHI GCGC 1 cut(s) 679
BstKTI GATC 3 cut(s) 120, 269, 573
BstMAI GTCTC 2 cut(s) 817, 847
BstMBI GATC 3 cut(s) 117, 266, 570
BstMWI GCNNNNNNNGC 3 cut(s) 25, 226, 683
BstNI CCWGG 1 cut(s) 618
BstNSI RCATGY 1 cut(s) 460
BstSCI CCNGG 1 cut(s) 616
BstSLI GKGCMC 2 cut(s) 30, 744
BstV2I GAAGAC 1 cut(s) 639
BstX2I RGATCY 2 cut(s) 117, 266
BstYI RGATCY 2 cut(s) 117, 266
BsuRI GGCC 2 cut(s) 474, 742
BtsCI GGATG 1 cut(s) 142
BtsI GCAGTG 1 cut(s) 62
BtsIMutI CAGTG 4 cut(s) 62, 557, 585, 668
Cac8I GCNNGC 4 cut(s) 30, 34, 69, 111
CaiI CAGNNNCTG 1 cut(s) 815
CciI TCATGA 1 cut(s) 694
CfoI GCGC 1 cut(s) 679
Cfr13I GGNCC 2 cut(s) 740, 741
Csp6I GTAC 2 cut(s) 450, 789
CviAII CATG 4 cut(s) 386, 457, 695, 910
CviQI GTAC 2 cut(s) 450, 789
DdeI CTNAG 4 cut(s) 114, 216, 370, 654
DpnI GATC 3 cut(s) 119, 268, 572
DpnII GATC 3 cut(s) 117, 266, 570
DraIII CACNNNGTG 1 cut(s) 68
Eam1104I CTCTTC 1 cut(s) 850
EarI CTCTTC 1 cut(s) 850
Ecl136II GAGCTC 1 cut(s) 527
Eco147I AGGCCT 1 cut(s) 474
Eco24I GRGCYC 2 cut(s) 529, 744
Eco31I GGTCTC 1 cut(s) 847
Eco47III AGCGCT 1 cut(s) 678
Eco53kI GAGCTC 1 cut(s) 527
Eco57I CTGAAG 1 cut(s) 206
EcoICRI GAGCTC 1 cut(s) 527
EcoO109I RGGNCCY 1 cut(s) 740
EcoRI GAATTC 1 cut(s) 201
EcoRII CCWGG 1 cut(s) 616
EcoT22I ATGCAT 1 cut(s) 609
EcoT38I GRGCYC 2 cut(s) 529, 744
FaeI CATG 4 cut(s) 389, 460, 698, 913
FalI AAGNNNNNCTT 2 cut(s) 871, 903
FaqI GGGAC 1 cut(s) 42
FatI CATG 4 cut(s) 385, 456, 694, 909
FauI CCCGC 1 cut(s) 37
FbaI TGATCA 1 cut(s) 570
FblI GTMKAC 3 cut(s) 346, 423, 535
FokI GGATG 1 cut(s) 149
FriOI GRGCYC 2 cut(s) 529, 744
FspBI CTAG 5 cut(s) 224, 350, 467, 786, 801
GlaI GCGC 1 cut(s) 678
GsuI CTGGAG 1 cut(s) 791
HaeII RGCGCY 1 cut(s) 680
HaeIII GGCC 2 cut(s) 474, 742
HhaI GCGC 1 cut(s) 679
Hin1II CATG 4 cut(s) 389, 460, 698, 913
Hin6I GCGC 1 cut(s) 677
HinP1I GCGC 1 cut(s) 677
HincII GTYRAC 1 cut(s) 424
HindII GTYRAC 1 cut(s) 424
HinfI GANTC 4 cut(s) 364, 400, 650, 698
Hpy166II GTNNAC 4 cut(s) 347, 424, 452, 536
Hpy188I TCNGA 5 cut(s) 186, 363, 649, 723, 895
Hpy188III TCNNGA 5 cut(s) 134, 224, 503, 695, 710
Hpy8I GTNNAC 4 cut(s) 347, 424, 452, 536
HpyAV CCTTC 2 cut(s) 464, 718
HpyCH4III ACNGT 3 cut(s) 261, 285, 811
HpyCH4IV ACGT 2 cut(s) 532, 538
HpyCH4V TGCA 9 cut(s) 71, 288, 322, 338, 411, 553, 607, 737, 833
HpyF10VI GCNNNNNNNGC 3 cut(s) 25, 226, 683
HpyF3I CTNAG 4 cut(s) 114, 216, 370, 654
HpySE526I ACGT 2 cut(s) 532, 538
Hsp92II CATG 4 cut(s) 389, 460, 698, 913
HspAI GCGC 1 cut(s) 677
Ksp22I TGATCA 1 cut(s) 570
Kzo9I GATC 3 cut(s) 117, 266, 570
LweI GCATC 4 cut(s) 127, 229, 315, 724
MaeI CTAG 5 cut(s) 224, 350, 467, 786, 801
MaeII ACGT 2 cut(s) 532, 538
MaeIII GTNAC 3 cut(s) 236, 300, 580
MalI GATC 3 cut(s) 119, 268, 572
MboI GATC 3 cut(s) 117, 266, 570
MboII GAAGA 6 cut(s) 43, 112, 199, 300, 644, 837
MflI RGATCY 2 cut(s) 117, 266
MhlI GDGCHC 3 cut(s) 30, 529, 744
MluCI AATT 6 cut(s) 201, 354, 494, 592, 641, 917
MmeI TCCRAC 1 cut(s) 709
MnlI CCTC 4 cut(s) 9, 53, 70, 72
Mph1103I ATGCAT 1 cut(s) 609
MseI TTAA 2 cut(s) 273, 925
MslI CAYNNNNRTG 1 cut(s) 578
MspA1I CMGCKG 1 cut(s) 545
MspR9I CCNGG 1 cut(s) 618
MvaI CCWGG 1 cut(s) 618
MwoI GCNNNNNNNGC 3 cut(s) 25, 226, 683
NdeII GATC 3 cut(s) 117, 266, 570
NlaIII CATG 4 cut(s) 389, 460, 698, 913
NlaIV GGNNCC 1 cut(s) 742
NmuCI GTSAC 1 cut(s) 580
NsiI ATGCAT 1 cut(s) 609
NspI RCATGY 1 cut(s) 460
PagI TCATGA 1 cut(s) 694
PceI AGGCCT 1 cut(s) 474
PciI ACATGT 1 cut(s) 456
PfeI GAWTC 4 cut(s) 364, 400, 650, 698
PscI ACATGT 1 cut(s) 456
PsiI TTATAA 1 cut(s) 431
Psp124BI GAGCTC 1 cut(s) 529
Psp6I CCWGG 1 cut(s) 616
PspGI CCWGG 1 cut(s) 616
PspN4I GGNNCC 1 cut(s) 742
PspOMI GGGCCC 1 cut(s) 740
PspPI GGNCC 2 cut(s) 740, 741
PstNI CAGNNNCTG 1 cut(s) 815
PsuI RGATCY 2 cut(s) 117, 266
PvuII CAGCTG 1 cut(s) 545
RsaI GTAC 2 cut(s) 451, 790
RsaNI GTAC 2 cut(s) 450, 789
RseI CAYNNNNRTG 1 cut(s) 578
SacI GAGCTC 1 cut(s) 529
SalI GTCGAC 1 cut(s) 422
SaqAI TTAA 2 cut(s) 273, 925
Sau3AI GATC 3 cut(s) 117, 266, 570
Sau96I GGNCC 2 cut(s) 740, 741
ScaI AGTACT 1 cut(s) 790
ScrFI CCNGG 1 cut(s) 618
SduI GDGCHC 3 cut(s) 30, 529, 744
SetI ASST 9 cut(s) 77, 468, 529, 535, 541, 547, 619, 729, 802
SfaNI GCATC 4 cut(s) 127, 229, 315, 724
SmiMI CAYNNNNRTG 1 cut(s) 578
SmlI CTYRAG 1 cut(s) 501
SmoI CTYRAG 1 cut(s) 501
Sse9I AATT 6 cut(s) 201, 354, 494, 592, 641, 917
SseBI AGGCCT 1 cut(s) 474
SsiI CCGC 3 cut(s) 30, 36, 397
SspMI CTAG 5 cut(s) 224, 350, 467, 786, 801
SstI GAGCTC 1 cut(s) 529
StuI AGGCCT 1 cut(s) 474
StyD4I CCNGG 1 cut(s) 616
TaaI ACNGT 3 cut(s) 261, 285, 811
TaiI ACGT 2 cut(s) 535, 541
TaqI TCGA 3 cut(s) 14, 77, 423
TasI AATT 6 cut(s) 201, 354, 494, 592, 641, 917
TatI WGTACW 2 cut(s) 449, 788
TfiI GAWTC 4 cut(s) 364, 400, 650, 698
Tru1I TTAA 2 cut(s) 273, 925
Tru9I TTAA 2 cut(s) 273, 925
TscAI CASTG 4 cut(s) 69, 564, 585, 668
TseFI GTSAC 1 cut(s) 580
Tsp45I GTSAC 1 cut(s) 580
TspDTI ATGAA 5 cut(s) 405, 534, 683, 690, 898
TspGWI ACGGA 1 cut(s) 651
TspRI CASTG 4 cut(s) 69, 564, 585, 668
XapI RAATTY 2 cut(s) 201, 917
XbaI TCTAGA 1 cut(s) 223
XceI RCATGY 1 cut(s) 460
XmiI GTMKAC 3 cut(s) 346, 423, 535
XspI CTAG 5 cut(s) 224, 350, 467, 786, 801
ZrmI AGTACT 1 cut(s) 790
Zsp2I ATGCAT 1 cut(s) 609
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.