MD03G1100400.v1.1

Protein of unknown function (DUF1635)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0002977
Physical Location & Seq
Forward (+)
8584853 .. 8587277
2425 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1100400.v1.1.491

Sequence Viewer

Length: 963 bp
ATGGAGGAACTGCGTTCTCTGTGGACTACTAATCCTGAGAGCGCTGAGGAGTTGAAGCAGAAGCTTCTGTACACAACCCTTGAGCTAGAATCTGTAAAAACACGCGCAACGGAAGAAATCCGGAAAAGCGAGGAGAATGTGAAGAAACTAGTCAATCTTCTAAACGTTGCATACAAAGAAAGACAAGATGCCGTGGATCAGCTACAAAAGCTCGTAAACAAGATCATGCCTTCAAGTCCAATGGAACTCCCCCTCAACATGTTTCCTCATGAAAGTACACTTTTGCTTCCCACCAAAGCAAACTCAAGCATAACTGAATCCAACAGCCTCTCTGAAACCTACAATCCCCAAAAATCGCACGCCTCTTCCTCTGTGGACTCCTTATTTGATGCGGTTTCTTCACCCGAGTTTTCAAACATGAACATGGCTGAATCTGGCTGCATCCCTTTAGCGAAGCAGCAGCCTCTGGTGCAAGAATATAATGGCATTGTATCTGCTGGTGTAGTCTCTTCAGGGATGATCAAACCCGACCCGGCTTCTCAGGTGATTGACAATTTTGTTAAAGGAAAGATTTTGCCTCAACAGGGCAAGCTCTTGCAGACTGTGATGCAAGCCGGTCCACTGCTCCAGACACTTCTTGTTTCCGGGCCAATGCCTCGTTGGCGAAACCCCCCTCCTTTGCAGCCCTTCAAAATCCCACCTGTTTCAATCCAAGGCTGTGAAGCTGCTGCAAGCTTCAACCAGAAACCAGTGGGAAATGCTAGTTATGTTGTCCATAAGCCAATCAACTCCGCGTCATATCCTGAGATGTCTCGCGGGTTTTCGCAGACATGTTCGGCTTCCTTGTTGAACTTCAACAACGGTGGTGCTTCTGTTTCATGCCTGAACAATGCAAGGATGTTGGCTTCGAATTCCGGCATTGACCACCAATTTTCGATTGGCAAGCGGCAGAGGCTTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1635

Protein Analysis

321

Amino Acids

35.03

Weight (kDa)

8.26

Isoelectric Point (pI)

52.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1635 PF07795 14 - 255 1.5e-46 Protein of unknown function (DUF1635)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 105, 794, 816
AccIII TCCGGA 1 cut(s) 120
AciI CCGC 4 cut(s) 392, 792, 816, 946
AclI AACGTT 1 cut(s) 165
AclWI GGATC 1 cut(s) 204
AcsI RAATTY 1 cut(s) 910
AcuI CTGAAG 1 cut(s) 495
AfaI GTAC 2 cut(s) 71, 277
AfeI AGCGCT 1 cut(s) 43
AfiI CCNNNNNNNGG 1 cut(s) 584
AflIII ACRYGT 2 cut(s) 258, 830
AgsI TTSAA 9 cut(s) 55, 234, 414, 691, 708, 739, 850, 856, 959
AhlI ACTAGT 1 cut(s) 148
AluBI AGCT 7 cut(s) 64, 85, 202, 211, 592, 725, 735
AluI AGCT 7 cut(s) 64, 85, 202, 211, 592, 725, 735
Alw26I GTCTC 2 cut(s) 511, 816
AlwI GGATC 1 cut(s) 204
AlwNI CAGNNNCTG 1 cut(s) 466
Ama87I CYCGRG 1 cut(s) 404
Aor13HI TCCGGA 1 cut(s) 120
Aor51HI AGCGCT 1 cut(s) 43
AoxI GGCC 1 cut(s) 647
ApeKI GCWGC 6 cut(s) 438, 457, 460, 682, 725, 728
ApoI RAATTY 1 cut(s) 910
ArsI GACNNNNNNTTYG 2 cut(s) 368, 400
AspLEI GCGC 2 cut(s) 44, 107
AspS9I GGNCC 2 cut(s) 617, 647
AsuC2I CCSGG 2 cut(s) 533, 646
AsuHPI GGTGA 2 cut(s) 393, 556
AsuII TTCGAA 1 cut(s) 908
AvaI CYCGRG 1 cut(s) 404
AvaII GGWCC 1 cut(s) 617
BarI GAAGNNNNNNTAC 2 cut(s) 53, 85
BbvCI CCTCAGC 1 cut(s) 45
BbvI GCAGC 6 cut(s) 425, 469, 472, 694, 712, 715
BceAI ACGGC 1 cut(s) 176
BclI TGATCA 1 cut(s) 519
BcnI CCSGG 2 cut(s) 533, 646
BcoDI GTCTC 2 cut(s) 511, 816
BcuI ACTAGT 1 cut(s) 148
BfaI CTAG 3 cut(s) 86, 149, 762
BfoI RGCGCY 1 cut(s) 45
BglI GCCNNNNNGGC 1 cut(s) 661
BisI GCNGC 7 cut(s) 439, 458, 461, 683, 726, 729, 947
BlsI GCNGC 7 cut(s) 440, 459, 462, 684, 727, 730, 948
Bme1390I CCNGG 2 cut(s) 533, 646
Bme18I GGWCC 1 cut(s) 617
BmeT110I CYCGRG 1 cut(s) 404
BmgT120I GGNCC 2 cut(s) 617, 647
BmrFI CCNGG 2 cut(s) 533, 646
BmsI GCATC 4 cut(s) 178, 379, 450, 597
BpmI CTGGAG 1 cut(s) 611
Bpu10I CCTNAGC 1 cut(s) 45
Bpu14I TTCGAA 1 cut(s) 908
BpuEI CTTGAG 2 cut(s) 101, 289
BpuMI CCSGG 2 cut(s) 533, 646
BsaJI CCNNGG 2 cut(s) 192, 712
BsaWI WCCGGW 1 cut(s) 120
Bsc4I CCNNNNNNNGG 1 cut(s) 584
Bse118I RCCGGY 1 cut(s) 614
Bse1I ACTGG 1 cut(s) 749
BseAI TCCGGA 1 cut(s) 120
BseDI CCNNGG 2 cut(s) 192, 712
BseGI GGATG 3 cut(s) 441, 522, 903
BseLI CCNNNNNNNGG 1 cut(s) 584
BseMII CTCAG 4 cut(s) 27, 36, 554, 795
BseNI ACTGG 1 cut(s) 749
BseRI GAGGAG 2 cut(s) 62, 146
BseXI GCAGC 6 cut(s) 425, 469, 472, 694, 712, 715
Bsh1236I CGCG 3 cut(s) 105, 794, 816
BshFI GGCC 1 cut(s) 649
BsiHKCI CYCGRG 1 cut(s) 404
BsiSI CCGG 5 cut(s) 121, 533, 615, 645, 915
BslI CCNNNNNNNGG 1 cut(s) 584
BsmAI GTCTC 2 cut(s) 511, 816
BsnI GGCC 1 cut(s) 649
BsoBI CYCGRG 1 cut(s) 404
Bsp119I TTCGAA 1 cut(s) 908
Bsp13I TCCGGA 1 cut(s) 120
Bsp1407I TGTACA 1 cut(s) 69
Bsp143I GATC 3 cut(s) 196, 222, 519
BspACI CCGC 4 cut(s) 392, 792, 816, 946
BspANI GGCC 1 cut(s) 649
BspCNI CTCAG 4 cut(s) 28, 37, 553, 796
BspEI TCCGGA 1 cut(s) 120
BspFNI CGCG 3 cut(s) 105, 794, 816
BspHI TCATGA 1 cut(s) 268
BspPI GGATC 1 cut(s) 204
BspT104I TTCGAA 1 cut(s) 908
BsrFI RCCGGY 1 cut(s) 614
BsrGI TGTACA 1 cut(s) 69
BsrI ACTGG 1 cut(s) 749
BssAI RCCGGY 1 cut(s) 614
BssECI CCNNGG 2 cut(s) 192, 712
BssMI GATC 3 cut(s) 196, 222, 519
BssT1I CCWWGG 1 cut(s) 712
Bst4CI ACNGT 2 cut(s) 604, 863
Bst6I CTCTTC 2 cut(s) 370, 514
BstAUI TGTACA 1 cut(s) 69
BstBI TTCGAA 1 cut(s) 908
BstC8I GCNNGC 5 cut(s) 360, 590, 612, 733, 944
BstDEI CTNAG 4 cut(s) 36, 45, 540, 804
BstDSI CCRYGG 1 cut(s) 192
BstF5I GGATG 3 cut(s) 441, 522, 903
BstFNI CGCG 3 cut(s) 105, 794, 816
BstH2I RGCGCY 1 cut(s) 45
BstHHI GCGC 2 cut(s) 44, 107
BstKTI GATC 3 cut(s) 199, 225, 522
BstMAI GTCTC 2 cut(s) 511, 816
BstMBI GATC 3 cut(s) 196, 222, 519
BstMWI GCNNNNNNNGC 4 cut(s) 208, 469, 661, 952
BstNSI RCATGY 2 cut(s) 262, 834
BstSCI CCNGG 2 cut(s) 531, 644
BstUI CGCG 3 cut(s) 105, 794, 816
BstV1I GCAGC 6 cut(s) 425, 469, 472, 694, 712, 715
BsuRI GGCC 1 cut(s) 649
BtgI CCRYGG 1 cut(s) 192
BtsCI GGATG 3 cut(s) 441, 522, 903
BtsI GCAGTG 1 cut(s) 620
BtsIMutI CAGTG 2 cut(s) 620, 756
Cac8I GCNNGC 5 cut(s) 360, 590, 612, 733, 944
CaiI CAGNNNCTG 1 cut(s) 466
CciI TCATGA 1 cut(s) 268
CfoI GCGC 2 cut(s) 44, 107
Cfr10I RCCGGY 1 cut(s) 614
Cfr13I GGNCC 2 cut(s) 617, 647
CseI GACGC 1 cut(s) 783
Csp6I GTAC 2 cut(s) 70, 276
CspCI CAANNNNNGTGG 2 cut(s) 844, 879
CviAII CATG 7 cut(s) 226, 259, 269, 418, 424, 831, 879
CviQI GTAC 2 cut(s) 70, 276
DdeI CTNAG 4 cut(s) 36, 45, 540, 804
DpnI GATC 3 cut(s) 198, 224, 521
DpnII GATC 3 cut(s) 196, 222, 519
Eam1104I CTCTTC 2 cut(s) 370, 514
EarI CTCTTC 2 cut(s) 370, 514
Eco130I CCWWGG 1 cut(s) 712
Eco47I GGWCC 1 cut(s) 617
Eco47III AGCGCT 1 cut(s) 43
Eco57I CTGAAG 1 cut(s) 495
Eco88I CYCGRG 1 cut(s) 404
EcoRI GAATTC 1 cut(s) 910
EcoT14I CCWWGG 1 cut(s) 712
ErhI CCWWGG 1 cut(s) 712
FaeI CATG 7 cut(s) 229, 262, 272, 421, 427, 834, 882
FatI CATG 7 cut(s) 225, 258, 268, 417, 423, 830, 878
FauI CCCGC 1 cut(s) 809
FbaI TGATCA 1 cut(s) 519
Fnu4HI GCNGC 7 cut(s) 439, 458, 461, 683, 726, 729, 947
FokI GGATG 3 cut(s) 428, 529, 910
Fsp4HI GCNGC 7 cut(s) 439, 458, 461, 683, 726, 729, 947
FspBI CTAG 3 cut(s) 86, 149, 762
GlaI GCGC 2 cut(s) 43, 106
GluI GCNGC 7 cut(s) 439, 458, 461, 683, 726, 729, 947
GsuI CTGGAG 1 cut(s) 611
HaeII RGCGCY 1 cut(s) 45
HaeIII GGCC 1 cut(s) 649
HapII CCGG 5 cut(s) 121, 533, 615, 645, 915
HgaI GACGC 1 cut(s) 783
HhaI GCGC 2 cut(s) 44, 107
Hin1II CATG 7 cut(s) 229, 262, 272, 421, 427, 834, 882
Hin6I GCGC 2 cut(s) 42, 105
HinP1I GCGC 2 cut(s) 42, 105
HindIII AAGCTT 2 cut(s) 62, 733
HinfI GANTC 4 cut(s) 89, 317, 377, 431
HpaII CCGG 5 cut(s) 121, 533, 615, 645, 915
HphI GGTGA 2 cut(s) 393, 556
Hpy166II GTNNAC 6 cut(s) 24, 72, 217, 278, 376, 620
Hpy188I TCNGA 1 cut(s) 334
Hpy188III TCNNGA 5 cut(s) 35, 121, 269, 628, 803
Hpy8I GTNNAC 6 cut(s) 24, 72, 217, 278, 376, 620
HpyAV CCTTC 2 cut(s) 240, 697
HpyCH4III ACNGT 2 cut(s) 604, 863
HpyCH4IV ACGT 1 cut(s) 165
HpyCH4V TGCA 8 cut(s) 170, 441, 472, 598, 610, 682, 731, 893
HpyF10VI GCNNNNNNNGC 4 cut(s) 208, 469, 661, 952
HpyF3I CTNAG 4 cut(s) 36, 45, 540, 804
HpySE526I ACGT 1 cut(s) 165
Hsp92II CATG 7 cut(s) 229, 262, 272, 421, 427, 834, 882
HspAI GCGC 2 cut(s) 42, 105
Kpn2I TCCGGA 1 cut(s) 120
Ksp22I TGATCA 1 cut(s) 519
Kzo9I GATC 3 cut(s) 196, 222, 519
LmnI GCTCC 1 cut(s) 630
Lsp1109I GCAGC 6 cut(s) 425, 469, 472, 694, 712, 715
LweI GCATC 4 cut(s) 178, 379, 450, 597
MaeI CTAG 3 cut(s) 86, 149, 762
MaeII ACGT 1 cut(s) 165
MalI GATC 3 cut(s) 198, 224, 521
MboI GATC 3 cut(s) 196, 222, 519
MboII GAAGA 6 cut(s) 125, 149, 154, 357, 390, 501
MluCI AATT 3 cut(s) 553, 910, 929
MlyI GAGTC 1 cut(s) 371
MmeI TCCRAC 1 cut(s) 345
MroI TCCGGA 1 cut(s) 120
MseI TTAA 1 cut(s) 561
MslI CAYNNNNRTG 1 cut(s) 422
MspI CCGG 5 cut(s) 121, 533, 615, 645, 915
MspR9I CCNGG 2 cut(s) 533, 646
MvnI CGCG 3 cut(s) 105, 794, 816
MwoI GCNNNNNNNGC 4 cut(s) 208, 469, 661, 952
NciI CCSGG 2 cut(s) 533, 646
NdeII GATC 3 cut(s) 196, 222, 519
NlaIII CATG 7 cut(s) 229, 262, 272, 421, 427, 834, 882
NspI RCATGY 2 cut(s) 262, 834
NspV TTCGAA 1 cut(s) 908
PagI TCATGA 1 cut(s) 268
PciI ACATGT 2 cut(s) 258, 830
PfeI GAWTC 3 cut(s) 89, 317, 431
PkrI GCNGC 7 cut(s) 440, 459, 462, 684, 727, 730, 948
PleI GAGTC 1 cut(s) 371
PpsI GAGTC 1 cut(s) 371
PscI ACATGT 2 cut(s) 258, 830
Psp1406I AACGTT 1 cut(s) 165
PspPI GGNCC 2 cut(s) 617, 647
PstNI CAGNNNCTG 1 cut(s) 466
RsaI GTAC 2 cut(s) 71, 277
RsaNI GTAC 2 cut(s) 70, 276
RseI CAYNNNNRTG 1 cut(s) 422
SaqAI TTAA 1 cut(s) 561
SatI GCNGC 7 cut(s) 439, 458, 461, 683, 726, 729, 947
Sau3AI GATC 3 cut(s) 196, 222, 519
Sau96I GGNCC 2 cut(s) 617, 647
SchI GAGTC 1 cut(s) 371
ScrFI CCNGG 2 cut(s) 533, 646
SfaNI GCATC 4 cut(s) 178, 379, 450, 597
SfuI TTCGAA 1 cut(s) 908
SinI GGWCC 1 cut(s) 617
SmiMI CAYNNNNRTG 1 cut(s) 422
SmlI CTYRAG 2 cut(s) 80, 304
SmoI CTYRAG 2 cut(s) 80, 304
SpeI ACTAGT 1 cut(s) 148
Sse9I AATT 3 cut(s) 553, 910, 929
SsiI CCGC 4 cut(s) 392, 792, 816, 946
SspMI CTAG 3 cut(s) 86, 149, 762
StyD4I CCNGG 2 cut(s) 531, 644
StyI CCWWGG 1 cut(s) 712
TaaI ACNGT 2 cut(s) 604, 863
TaiI ACGT 1 cut(s) 168
TaqI TCGA 2 cut(s) 908, 935
TasI AATT 3 cut(s) 553, 910, 929
TatI WGTACW 2 cut(s) 69, 275
TauI GCSGC 1 cut(s) 949
TfiI GAWTC 3 cut(s) 89, 317, 431
Tru1I TTAA 1 cut(s) 561
Tru9I TTAA 1 cut(s) 561
TscAI CASTG 2 cut(s) 627, 756
TseI GCWGC 6 cut(s) 438, 457, 460, 682, 725, 728
TspDTI ATGAA 3 cut(s) 285, 434, 867
TspGWI ACGGA 1 cut(s) 125
TspRI CASTG 2 cut(s) 627, 756
VpaK11BI GGWCC 1 cut(s) 617
XapI RAATTY 1 cut(s) 910
XceI RCATGY 2 cut(s) 262, 834
XcmI CCANNNNNNNNNTGG 2 cut(s) 657, 935
XspI CTAG 3 cut(s) 86, 149, 762
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.