MD11G1114400.v1.1

Protein of unknown function (DUF1635)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0002977
Physical Location & Seq
Forward (+)
10361048 .. 10363363
2316 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1114400.v1.1.491

Sequence Viewer

Length: 960 bp
ATGGAGGAACTGGATTCTCTGTGGACTACTTGCCCTGAGAGCGCTGATGAGTTGAAGGAGAAGCTTCTGTACACAACCCTTGAGCTAGAATCAGTAAAAACACGCGCAACGGATGAAATCCGAAAAAGCGAGGAGAATGTGAAGAAACTAGTCAATCTTCTAAACGTTGCATCCAAAGAAAGAGATGATGCTGTAGATCAGCTACGAAAGCTGGTAAACAAGATCATGCCTTCAGGTCCAATGGAACTCCCCCTCAACATGTTTCCTCATGAAAGTACACTTTTGCTTCCCACCAAGGCAAACTCAAGCATAACTGAATCCAACAGCCTCTCTGAAACCTACAATCCCCAAAAATCGCACGTCTCTTCCTCTGTGGACTCCTTATTCGATGCCGTTTCTTCACCCGAGTTTTCGAACATAAACATGGCTGAATCCGGCTGCATTCCTTTTGTGAAGCAGCAGCCTCGGGTGCAAGAATATAATGGTACTGCATCTGCTGGTGTAGTCTCAGGGATGATCAAACCCGACCCGGCTTCTGAAGTGATTGACAACTTTGTGAAAGGAAAGATTTTGCCTAAACAGGGAAAGCTCTTGCAGACTGTGATGGAAGCCGGTCCACTGCTCGAGACTCTTCTTGTTGCTGGGCCAATGCCTCGTTGGCGAAACCCCCCGCCTTTGCAGCCCTTCAAAATCCCACCTTTTTCAATCAAAGGCTGTGAAGCTGCTGCAAGCTTTAACCAGAAACCAGTGGGAAATACTAGTTATGTTGTCCATAAGCCAATCAACTCGGCATCATATCCTGAGATGTCTCGCGGCTTTTCGCAGACATGTTCGGCTTCCATGTTGAACTTCAACAATGGTGGTGCTTCCATCTCACGCCTGAACAATGCAAGGATGTTGGCTTCAAATTCCGGCATTGACCACCAATTTTCGATTGGCAAGCGGCAGAGGCTTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1635

Protein Analysis

320

Amino Acids

35.0

Weight (kDa)

6.92

Isoelectric Point (pI)

43.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1635 PF07795 14 - 255 1.8e-47 Protein of unknown function (DUF1635)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 105, 813
AciI CCGC 3 cut(s) 671, 813, 943
AclI AACGTT 1 cut(s) 165
AcsI RAATTY 1 cut(s) 907
AcuI CTGAAG 2 cut(s) 216, 558
AfaI GTAC 3 cut(s) 71, 277, 487
AfeI AGCGCT 1 cut(s) 43
AfiI CCNNNNNNNGG 1 cut(s) 581
AflIII ACRYGT 2 cut(s) 258, 827
AgsI TTSAA 7 cut(s) 55, 688, 705, 847, 853, 906, 956
AhlI ACTAGT 2 cut(s) 148, 758
AjiI CACGTC 1 cut(s) 361
AluBI AGCT 7 cut(s) 64, 85, 202, 211, 589, 722, 732
AluI AGCT 7 cut(s) 64, 85, 202, 211, 589, 722, 732
Alw26I GTCTC 4 cut(s) 367, 511, 620, 813
Ama87I CYCGRG 3 cut(s) 404, 465, 623
Aor51HI AGCGCT 1 cut(s) 43
AoxI GGCC 1 cut(s) 644
ApeKI GCWGC 6 cut(s) 438, 457, 460, 679, 722, 725
ApoI RAATTY 1 cut(s) 907
ArsI GACNNNNNNTTYG 2 cut(s) 368, 400
AspLEI GCGC 2 cut(s) 44, 107
AspS9I GGNCC 3 cut(s) 236, 614, 644
AsuC2I CCSGG 1 cut(s) 530
AsuHPI GGTGA 1 cut(s) 393
AsuII TTCGAA 1 cut(s) 413
AvaI CYCGRG 3 cut(s) 404, 465, 623
AvaII GGWCC 2 cut(s) 236, 614
BarI GAAGNNNNNNTAC 2 cut(s) 53, 85
BbvI GCAGC 6 cut(s) 425, 469, 472, 691, 709, 712
BccI CCATC 2 cut(s) 598, 878
BceAI ACGGC 1 cut(s) 377
BcgI CGANNNNNNTGC 2 cut(s) 446, 480
BclI TGATCA 1 cut(s) 516
BcnI CCSGG 1 cut(s) 530
BcoDI GTCTC 4 cut(s) 367, 511, 620, 813
BcuI ACTAGT 2 cut(s) 148, 758
BfaI CTAG 3 cut(s) 86, 149, 759
BfmI CTRYAG 1 cut(s) 192
BfoI RGCGCY 1 cut(s) 45
BglI GCCNNNNNGGC 1 cut(s) 658
BisI GCNGC 8 cut(s) 439, 458, 461, 680, 723, 726, 814, 944
BlsI GCNGC 8 cut(s) 440, 459, 462, 681, 724, 727, 815, 945
Bme1390I CCNGG 1 cut(s) 530
Bme18I GGWCC 2 cut(s) 236, 614
BmeT110I CYCGRG 3 cut(s) 404, 465, 623
BmgBI CACGTC 1 cut(s) 361
BmgT120I GGNCC 3 cut(s) 236, 614, 644
BmrFI CCNGG 1 cut(s) 530
BmsI GCATC 5 cut(s) 178, 179, 379, 500, 800
Bpu14I TTCGAA 1 cut(s) 413
BpuEI CTTGAG 2 cut(s) 101, 289
BpuMI CCSGG 1 cut(s) 530
BsaJI CCNNGG 2 cut(s) 294, 464
Bsc4I CCNNNNNNNGG 1 cut(s) 581
Bse118I RCCGGY 1 cut(s) 611
Bse1I ACTGG 2 cut(s) 15, 746
BseDI CCNNGG 2 cut(s) 294, 464
BseGI GGATG 4 cut(s) 118, 170, 519, 900
BseLI CCNNNNNNNGG 1 cut(s) 581
BseMII CTCAG 3 cut(s) 27, 522, 792
BseNI ACTGG 2 cut(s) 15, 746
BseRI GAGGAG 1 cut(s) 146
BseXI GCAGC 6 cut(s) 425, 469, 472, 691, 709, 712
BseYI CCCAGC 1 cut(s) 641
Bsh1236I CGCG 2 cut(s) 105, 813
BshFI GGCC 1 cut(s) 646
BsiHKCI CYCGRG 3 cut(s) 404, 465, 623
BsiSI CCGG 4 cut(s) 435, 530, 612, 912
BslI CCNNNNNNNGG 1 cut(s) 581
BsmAI GTCTC 4 cut(s) 367, 511, 620, 813
BsmBI CGTCTC 1 cut(s) 367
BsmI GAATGC 1 cut(s) 441
BsnI GGCC 1 cut(s) 646
BsoBI CYCGRG 3 cut(s) 404, 465, 623
Bsp119I TTCGAA 1 cut(s) 413
Bsp1407I TGTACA 1 cut(s) 69
Bsp143I GATC 3 cut(s) 196, 222, 516
BspACI CCGC 3 cut(s) 671, 813, 943
BspANI GGCC 1 cut(s) 646
BspCNI CTCAG 3 cut(s) 28, 521, 793
BspFNI CGCG 2 cut(s) 105, 813
BspHI TCATGA 1 cut(s) 268
BspT104I TTCGAA 1 cut(s) 413
BsrFI RCCGGY 1 cut(s) 611
BsrGI TGTACA 1 cut(s) 69
BsrI ACTGG 2 cut(s) 15, 746
BssAI RCCGGY 1 cut(s) 611
BssECI CCNNGG 2 cut(s) 294, 464
BssMI GATC 3 cut(s) 196, 222, 516
BssT1I CCWWGG 1 cut(s) 294
Bst4CI ACNGT 1 cut(s) 601
Bst6I CTCTTC 2 cut(s) 370, 636
BstAUI TGTACA 1 cut(s) 69
BstBI TTCGAA 1 cut(s) 413
BstC8I GCNNGC 2 cut(s) 730, 941
BstDEI CTNAG 3 cut(s) 36, 508, 801
BstF5I GGATG 4 cut(s) 118, 170, 519, 900
BstFNI CGCG 2 cut(s) 105, 813
BstH2I RGCGCY 1 cut(s) 45
BstHHI GCGC 2 cut(s) 44, 107
BstKTI GATC 3 cut(s) 199, 225, 519
BstMAI GTCTC 4 cut(s) 367, 511, 620, 813
BstMBI GATC 3 cut(s) 196, 222, 516
BstMWI GCNNNNNNNGC 6 cut(s) 39, 208, 469, 658, 679, 949
BstNSI RCATGY 2 cut(s) 262, 831
BstSCI CCNGG 1 cut(s) 528
BstSFI CTRYAG 1 cut(s) 192
BstUI CGCG 2 cut(s) 105, 813
BstV1I GCAGC 6 cut(s) 425, 469, 472, 691, 709, 712
BsuRI GGCC 1 cut(s) 646
BtrI CACGTC 1 cut(s) 361
BtsCI GGATG 4 cut(s) 118, 170, 519, 900
BtsI GCAGTG 1 cut(s) 617
BtsIMutI CAGTG 2 cut(s) 617, 753
Cac8I GCNNGC 2 cut(s) 730, 941
CciI TCATGA 1 cut(s) 268
CfoI GCGC 2 cut(s) 44, 107
Cfr10I RCCGGY 1 cut(s) 611
Cfr13I GGNCC 3 cut(s) 236, 614, 644
Csp6I GTAC 3 cut(s) 70, 276, 486
CspCI CAANNNNNGTGG 2 cut(s) 841, 876
CviAII CATG 6 cut(s) 226, 259, 269, 424, 828, 841
CviQI GTAC 3 cut(s) 70, 276, 486
DdeI CTNAG 3 cut(s) 36, 508, 801
DpnI GATC 3 cut(s) 198, 224, 518
DpnII GATC 3 cut(s) 196, 222, 516
Eam1104I CTCTTC 2 cut(s) 370, 636
EarI CTCTTC 2 cut(s) 370, 636
Eco130I CCWWGG 1 cut(s) 294
Eco47I GGWCC 2 cut(s) 236, 614
Eco47III AGCGCT 1 cut(s) 43
Eco57I CTGAAG 2 cut(s) 216, 558
Eco88I CYCGRG 3 cut(s) 404, 465, 623
EcoT14I CCWWGG 1 cut(s) 294
ErhI CCWWGG 1 cut(s) 294
Esp3I CGTCTC 1 cut(s) 367
FaeI CATG 6 cut(s) 229, 262, 272, 427, 831, 844
FatI CATG 6 cut(s) 225, 258, 268, 423, 827, 840
FauI CCCGC 1 cut(s) 678
FbaI TGATCA 1 cut(s) 516
Fnu4HI GCNGC 8 cut(s) 439, 458, 461, 680, 723, 726, 814, 944
FokI GGATG 4 cut(s) 125, 157, 526, 907
Fsp4HI GCNGC 8 cut(s) 439, 458, 461, 680, 723, 726, 814, 944
FspBI CTAG 3 cut(s) 86, 149, 759
GlaI GCGC 2 cut(s) 43, 106
GluI GCNGC 8 cut(s) 439, 458, 461, 680, 723, 726, 814, 944
GsaI CCCAGC 1 cut(s) 645
HaeII RGCGCY 1 cut(s) 45
HaeIII GGCC 1 cut(s) 646
HapII CCGG 4 cut(s) 435, 530, 612, 912
HhaI GCGC 2 cut(s) 44, 107
Hin1II CATG 6 cut(s) 229, 262, 272, 427, 831, 844
Hin6I GCGC 2 cut(s) 42, 105
HinP1I GCGC 2 cut(s) 42, 105
HindIII AAGCTT 2 cut(s) 62, 730
HinfI GANTC 6 cut(s) 14, 89, 317, 377, 431, 628
HpaII CCGG 4 cut(s) 435, 530, 612, 912
HphI GGTGA 1 cut(s) 393
Hpy166II GTNNAC 6 cut(s) 24, 72, 217, 278, 376, 617
Hpy188I TCNGA 3 cut(s) 122, 334, 538
Hpy188III TCNNGA 3 cut(s) 269, 625, 800
Hpy8I GTNNAC 6 cut(s) 24, 72, 217, 278, 376, 617
HpyAV CCTTC 3 cut(s) 49, 240, 694
HpyCH4III ACNGT 1 cut(s) 601
HpyCH4IV ACGT 2 cut(s) 165, 360
HpyCH4V TGCA 8 cut(s) 170, 441, 472, 491, 595, 679, 728, 890
HpyF10VI GCNNNNNNNGC 6 cut(s) 39, 208, 469, 658, 679, 949
HpyF3I CTNAG 3 cut(s) 36, 508, 801
HpySE526I ACGT 2 cut(s) 165, 360
Hsp92II CATG 6 cut(s) 229, 262, 272, 427, 831, 844
HspAI GCGC 2 cut(s) 42, 105
Ksp22I TGATCA 1 cut(s) 516
Kzo9I GATC 3 cut(s) 196, 222, 516
Lsp1109I GCAGC 6 cut(s) 425, 469, 472, 691, 709, 712
LweI GCATC 5 cut(s) 178, 179, 379, 500, 800
MaeI CTAG 3 cut(s) 86, 149, 759
MaeII ACGT 2 cut(s) 165, 360
MalI GATC 3 cut(s) 198, 224, 518
MboI GATC 3 cut(s) 196, 222, 516
MboII GAAGA 5 cut(s) 149, 154, 357, 390, 623
MluCI AATT 2 cut(s) 907, 926
MlyI GAGTC 2 cut(s) 371, 622
MmeI TCCRAC 1 cut(s) 345
MnlI CCTC 8 cut(s) 124, 263, 276, 338, 379, 474, 663, 942
MseI TTAA 1 cut(s) 735
MslI CAYNNNNRTG 1 cut(s) 422
MspI CCGG 4 cut(s) 435, 530, 612, 912
MspR9I CCNGG 1 cut(s) 530
Mva1269I GAATGC 1 cut(s) 441
MvnI CGCG 2 cut(s) 105, 813
MwoI GCNNNNNNNGC 6 cut(s) 39, 208, 469, 658, 679, 949
NciI CCSGG 1 cut(s) 530
NdeII GATC 3 cut(s) 196, 222, 516
NlaIII CATG 6 cut(s) 229, 262, 272, 427, 831, 844
NmeAIII GCCGAG 1 cut(s) 767
NspI RCATGY 2 cut(s) 262, 831
NspV TTCGAA 1 cut(s) 413
PaeR7I CTCGAG 1 cut(s) 623
PagI TCATGA 1 cut(s) 268
PciI ACATGT 2 cut(s) 258, 827
PctI GAATGC 1 cut(s) 441
PfeI GAWTC 4 cut(s) 14, 89, 317, 431
PkrI GCNGC 8 cut(s) 440, 459, 462, 681, 724, 727, 815, 945
PleI GAGTC 2 cut(s) 371, 622
PpsI GAGTC 2 cut(s) 371, 622
PscI ACATGT 2 cut(s) 258, 827
Psp1406I AACGTT 1 cut(s) 165
PspFI CCCAGC 1 cut(s) 641
PspPI GGNCC 3 cut(s) 236, 614, 644
RsaI GTAC 3 cut(s) 71, 277, 487
RsaNI GTAC 3 cut(s) 70, 276, 486
RseI CAYNNNNRTG 1 cut(s) 422
SaqAI TTAA 1 cut(s) 735
SatI GCNGC 8 cut(s) 439, 458, 461, 680, 723, 726, 814, 944
Sau3AI GATC 3 cut(s) 196, 222, 516
Sau96I GGNCC 3 cut(s) 236, 614, 644
SchI GAGTC 2 cut(s) 371, 622
ScrFI CCNGG 1 cut(s) 530
SfaNI GCATC 5 cut(s) 178, 179, 379, 500, 800
SfcI CTRYAG 1 cut(s) 192
Sfr274I CTCGAG 1 cut(s) 623
SfuI TTCGAA 1 cut(s) 413
SinI GGWCC 2 cut(s) 236, 614
SlaI CTCGAG 1 cut(s) 623
SmiMI CAYNNNNRTG 1 cut(s) 422
SmlI CTYRAG 3 cut(s) 80, 304, 623
SmoI CTYRAG 3 cut(s) 80, 304, 623
SpeI ACTAGT 2 cut(s) 148, 758
Sse9I AATT 2 cut(s) 907, 926
SsiI CCGC 3 cut(s) 671, 813, 943
SspMI CTAG 3 cut(s) 86, 149, 759
StyD4I CCNGG 1 cut(s) 528
StyI CCWWGG 1 cut(s) 294
TaaI ACNGT 1 cut(s) 601
TaiI ACGT 2 cut(s) 168, 363
TaqI TCGA 4 cut(s) 387, 413, 624, 932
TasI AATT 2 cut(s) 907, 926
TatI WGTACW 2 cut(s) 69, 275
TauI GCSGC 2 cut(s) 816, 946
TfiI GAWTC 4 cut(s) 14, 89, 317, 431
Tru1I TTAA 1 cut(s) 735
Tru9I TTAA 1 cut(s) 735
TscAI CASTG 2 cut(s) 624, 753
TseI GCWGC 6 cut(s) 438, 457, 460, 679, 722, 725
TspDTI ATGAA 2 cut(s) 129, 285
TspGWI ACGGA 1 cut(s) 125
TspRI CASTG 2 cut(s) 624, 753
VpaK11BI GGWCC 2 cut(s) 236, 614
XapI RAATTY 1 cut(s) 907
XceI RCATGY 2 cut(s) 262, 831
XcmI CCANNNNNNNNNTGG 2 cut(s) 654, 932
XhoI CTCGAG 1 cut(s) 623
XspI CTAG 3 cut(s) 86, 149, 759
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.