MD03G1141900.v1.1

Belongs to the AAA ATPase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0002427
Physical Location & Seq
Reverse (-)
15083942 .. 15088166
4225 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1141900.v1.1.491

Sequence Viewer

Length: 1302 bp
ATGTATAGCAATTTCAAGGAGCAAGCGATTGAGTACGTGAAGCAAGCCGTGCAAGAAGATAATGCGGGAAATTACGCCAAGGCGTTCCCTTTGTACATGAACGCGTTGGACTACTTCAAGACCCACTTGAAGTACGAGAAGAACCCTAAGATCAAGGAAGCAATTACCCAGAAGTTCACCGAGTACTTGCGCCGCGCCGAGGAGATTCGGGCGGTATTGGATGACGGAGGGCCGGGCCCGGCGTCTAACGGGGATGCGGCGGTGGCCACCAGGCCCAAGACCAAGCCCAAGGATGGGGAAGGCGGCGATGGGGAAGATCCGGAGCAGGCGAAGCTCCGGGCAGGGCTGAATTCGGCGATTATAAGGGAGAAGCCCAATGTGCAATGGAATGATGTTGCTGGTTTGGAGAGTGCCAAGCAGGCCTTGCAAGAGGCGGTTATATTGCCGGTCAAATTCCCGCAGTTCTTTACTGGCAAGAGACGGCCATGGAGGGCTTTTCTATTGTATGGGCCTCCTGGAACTGGAAAGTCTTACTTGGCCAAGGCTGTTGCAACTGAGGCGGACTCGACATTTTTCAGTATTTCTTCTTCAGACCTGGTTTCAAAGTGGATGGGTGAGAGTGAAAAGCTAGTTTCAAACCTTTTCCAAATGGCTCGTGATAGTGCACCATCCATTATATTCATCGATGAGATAGATTCCTTGTGTGGGACTCGTGGAGAAGGCAATGAGAGTGAAGCTTCTAGACGAATTAAGACGGAACTTCTTGTGCAGATGCAGGGTGTGGGAACCAATGATCAGAAAGTTCTTGTTCTCGCAGCAACAAATACTCCATATGCACTAGATCAGGCCATTCGGCGCCGTTTCGACAAGCGTATATACATTCCCCTCCCAGATTTAAAGGCTCGTCAGCATATGTTCAAAGTTCATCTAGGAGATACTCCTCACAACTTAACAGAAAGTGATTATGAAAGCTTAGCTGGCAAAACTGATGGTTTTTCAGGTTCCGATATTGCTGTTTGTGTTAAAGATGTTCTCTTTGAACCTGTTCGTAAAACGCAAGATGCCATGTTCTTTATCAAGGATCCTAGGGATATGTGGATACCATGTGGACCAAAGCAATCAGGTGCTGTCCAAATCACCATGCAGGAGCTGGCGGCAAAAGGACTCGCTTCACAGATCCTTCCGCCTCCTATCACAAAAATAGATTTCGATAAGGTGCTTGCTAAACAGAGGCCTACAGTGAGCAAAAGTGACCTTGACGTCCATGAGAGGTTCACAAGAGAGTTCGGAGAGGAAGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000902 GO:0000904 GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005770 GO:0005771 GO:0005911 GO:0006629 GO:0006810 GO:0006900 GO:0006914 GO:0006996 GO:0007032 GO:0007033 GO:0007034 GO:0008104 GO:0008144 GO:0008150 GO:0008152 GO:0008202 GO:0009056 GO:0009506 GO:0009653 GO:0009888 GO:0009987 GO:0010008 GO:0010026 GO:0010090 GO:0010091 GO:0010256 GO:0012505 GO:0016020 GO:0016043 GO:0016050 GO:0016125 GO:0016192 GO:0016197 GO:0016236 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017076 GO:0017111 GO:0022607 GO:0030054 GO:0030154 GO:0030554 GO:0031090 GO:0031410 GO:0031982 GO:0032502 GO:0032507 GO:0032509 GO:0032511 GO:0032553 GO:0032555 GO:0032559 GO:0032989 GO:0032991 GO:0033036 GO:0033365 GO:0034067 GO:0034613 GO:0035639 GO:0036094 GO:0036452 GO:0042592 GO:0042802 GO:0042803 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044422 GO:0044424 GO:0044425 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0045053 GO:0045185 GO:0045324 GO:0046907 GO:0046983 GO:0048468 GO:0048856 GO:0048869 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0051235 GO:0051259 GO:0051260 GO:0051641 GO:0051649 GO:0051651 GO:0055044 GO:0055065 GO:0055067 GO:0055075 GO:0055078 GO:0055080 GO:0061024 GO:0061919 GO:0065003 GO:0065007 GO:0065008 GO:0070676 GO:0070727 GO:0071704 GO:0071840 GO:0071985 GO:0090558 GO:0090626 GO:0097159 GO:0097367 GO:0097708 GO:0098588 GO:0098771 GO:0098796 GO:0098805 GO:1901265 GO:1901360 GO:1901363 GO:1901615 GO:1902494 GO:1904949 GO:1990621
KEGG Pathways
Metabolic & Signaling
ko04144 ko04217 map04144 map04217
Pfam Domains
Protein Families
AAA MIT Vps4_C

Protein Analysis

434

Amino Acids

48.16

Weight (kDa)

6.58

Isoelectric Point (pI)

40.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIT PF04212 7 - 70 4.1e-22 MIT (microtubule interacting and transport) domain
AAA PF00004 167 - 296 5.6e-44 ATPase family associated with various cellular activities (AAA)
AAA_lid_3 PF17862 321 - 354 3.6e-06 AAA+ lid domain
Vps4_C PF09336 366 - 430 5.5e-21 Vps4 C terminal oligomerisation domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 362
AasI GACNNNNNNGTC 1 cut(s) 1259
AatII GACGTC 1 cut(s) 1263
AccB1I GGYRCC 1 cut(s) 855
AccII CGCG 2 cut(s) 104, 195
AccIII TCCGGA 1 cut(s) 319
AclWI GGATC 4 cut(s) 311, 1076, 1089, 1171
AcoI YGGCCR 3 cut(s) 264, 482, 537
AcsI RAATTY 2 cut(s) 349, 452
AcuI CTGAAG 1 cut(s) 573
AcyI GRCGYC 3 cut(s) 242, 856, 1260
AfaI GTAC 4 cut(s) 35, 95, 134, 185
AfiI CCNNNNNNNGG 5 cut(s) 199, 293, 294, 521, 705
AflIII ACRYGT 1 cut(s) 102
AgsI TTSAA 7 cut(s) 16, 118, 130, 603, 636, 919, 1040
AjnI CCWGG 3 cut(s) 269, 514, 594
AluBI AGCT 6 cut(s) 334, 628, 737, 972, 977, 1150
AluI AGCT 6 cut(s) 334, 628, 737, 972, 977, 1150
Alw21I GWGCWC 1 cut(s) 667
Alw26I GTCTC 1 cut(s) 472
Alw44I GTGCAC 1 cut(s) 663
AlwI GGATC 4 cut(s) 311, 1076, 1089, 1171
AlwNI CAGNNNCTG 2 cut(s) 1127, 1150
Aor13HI TCCGGA 1 cut(s) 319
ApaI GGGCCC 1 cut(s) 239
ApaLI GTGCAC 1 cut(s) 663
ApeKI GCWGC 1 cut(s) 815
ApoI RAATTY 2 cut(s) 349, 452
Asp700I GAANNNNTTC 1 cut(s) 1044
AspA2I CCTAGG 1 cut(s) 1085
AspLEI GCGC 3 cut(s) 192, 197, 858
AspS9I GGNCC 6 cut(s) 230, 235, 236, 273, 509, 1109
AsuC2I CCSGG 3 cut(s) 234, 239, 338
AsuHPI GGTGA 3 cut(s) 169, 626, 1129
AvaII GGWCC 1 cut(s) 1109
AvrII CCTAGG 1 cut(s) 1085
BaeGI GKGCMC 2 cut(s) 239, 667
BalI TGGCCA 2 cut(s) 266, 539
BamHI GGATCC 1 cut(s) 1081
BanI GGYRCC 1 cut(s) 855
BanII GRGCYC 1 cut(s) 239
BarI GAAGNNNNNNTAC 2 cut(s) 571, 603
BauI CACGAG 2 cut(s) 654, 711
Bbv12I GWGCWC 1 cut(s) 667
BbvI GCAGC 1 cut(s) 827
BccI CCATC 5 cut(s) 287, 302, 604, 676, 983
BceAI ACGGC 3 cut(s) 32, 497, 843
BciT130I CCWGG 3 cut(s) 271, 516, 596
BciVI GTATCC 1 cut(s) 1092
BclI TGATCA 1 cut(s) 793
BcnI CCSGG 3 cut(s) 234, 239, 338
BcoDI GTCTC 1 cut(s) 472
BfaI CTAG 5 cut(s) 629, 741, 839, 929, 1086
BfmI CTRYAG 1 cut(s) 1236
BfoI RGCGCY 1 cut(s) 859
BfuI GTATCC 1 cut(s) 1092
BglI GCCNNNNNGGC 1 cut(s) 419
BisI GCNGC 5 cut(s) 193, 258, 304, 816, 1155
BlnI CCTAGG 1 cut(s) 1085
BlpI GCTNAGC 1 cut(s) 973
BlsI GCNGC 5 cut(s) 194, 259, 305, 817, 1156
BmcAI AGTACT 1 cut(s) 185
Bme1390I CCNGG 6 cut(s) 234, 239, 271, 338, 516, 596
Bme18I GGWCC 1 cut(s) 1109
BmgT120I GGNCC 6 cut(s) 230, 235, 236, 273, 509, 1109
BmiI GGNNCC 5 cut(s) 237, 787, 857, 1003, 1083
BmrFI CCNGG 6 cut(s) 234, 239, 271, 338, 516, 596
BmsI GCATC 3 cut(s) 244, 762, 1051
BplI GAGNNNNNCTC 2 cut(s) 548, 580
Bpu1102I GCTNAGC 1 cut(s) 973
BpuMI CCSGG 3 cut(s) 234, 239, 338
Bsa29I ATCGAT 1 cut(s) 684
BsaAI YACGTR 1 cut(s) 37
BsaHI GRCGYC 3 cut(s) 242, 856, 1260
BsaJI CCNNGG 6 cut(s) 78, 198, 288, 485, 540, 1085
BsaWI WCCGGW 1 cut(s) 319
BsaXI ACNNNNNCTCC 2 cut(s) 811, 841
Bsc4I CCNNNNNNNGG 5 cut(s) 199, 293, 294, 521, 705
Bse118I RCCGGY 1 cut(s) 445
Bse1I ACTGG 2 cut(s) 475, 526
Bse3DI GCAATG 2 cut(s) 389, 730
BseAI TCCGGA 1 cut(s) 319
BseBI CCWGG 3 cut(s) 271, 516, 596
BseCI ATCGAT 1 cut(s) 684
BseDI CCNNGG 6 cut(s) 78, 198, 288, 485, 540, 1085
BseGI GGATG 5 cut(s) 226, 259, 298, 615, 668
BseLI CCNNNNNNNGG 5 cut(s) 199, 293, 294, 521, 705
BseMI GCAATG 2 cut(s) 389, 730
BseMII CTCAG 1 cut(s) 546
BseNI ACTGG 2 cut(s) 475, 526
BseRI GAGGAG 2 cut(s) 215, 930
BseSI GKGCMC 2 cut(s) 239, 667
BseXI GCAGC 1 cut(s) 827
BsgI GTGCAG 1 cut(s) 788
Bsh1236I CGCG 2 cut(s) 104, 195
BshNI GGYRCC 1 cut(s) 855
BshVI ATCGAT 1 cut(s) 684
BsiHKAI GWGCWC 1 cut(s) 667
BsiSI CCGG 5 cut(s) 233, 239, 320, 337, 446
BslFI GGGAC 1 cut(s) 721
BslI CCNNNNNNNGG 5 cut(s) 199, 293, 294, 521, 705
BsmAI GTCTC 1 cut(s) 472
BsmBI CGTCTC 1 cut(s) 472
BsmFI GGGAC 1 cut(s) 721
Bsp120I GGGCCC 1 cut(s) 235
Bsp1286I GDGCHC 2 cut(s) 239, 667
Bsp13I TCCGGA 1 cut(s) 319
Bsp1407I TGTACA 1 cut(s) 93
Bsp143I GATC 6 cut(s) 150, 316, 793, 841, 1081, 1176
Bsp1720I GCTNAGC 1 cut(s) 973
Bsp19I CCATGG 1 cut(s) 485
BspCNI CTCAG 1 cut(s) 547
BspDI ATCGAT 1 cut(s) 684
BspEI TCCGGA 1 cut(s) 319
BspFNI CGCG 2 cut(s) 104, 195
BspLI GGNNCC 5 cut(s) 237, 787, 857, 1003, 1083
BspPI GGATC 4 cut(s) 311, 1076, 1089, 1171
BspT107I GGYRCC 1 cut(s) 855
BsrDI GCAATG 2 cut(s) 389, 730
BsrFI RCCGGY 1 cut(s) 445
BsrGI TGTACA 1 cut(s) 93
BsrI ACTGG 2 cut(s) 475, 526
BssAI RCCGGY 1 cut(s) 445
BssECI CCNNGG 6 cut(s) 78, 198, 288, 485, 540, 1085
BssMI GATC 6 cut(s) 150, 316, 793, 841, 1081, 1176
BssNI GRCGYC 3 cut(s) 242, 856, 1260
BssSI CACGAG 2 cut(s) 654, 711
BssT1I CCWWGG 5 cut(s) 78, 288, 485, 540, 1085
Bst2BI CACGAG 2 cut(s) 654, 711
Bst2UI CCWGG 3 cut(s) 271, 516, 596
Bst4CI ACNGT 1 cut(s) 1240
BstACI GRCGYC 3 cut(s) 242, 856, 1260
BstAPI GCANNNNNTGC 2 cut(s) 49, 424
BstAUI TGTACA 1 cut(s) 93
BstBAI YACGTR 1 cut(s) 37
BstC8I GCNNGC 7 cut(s) 24, 45, 327, 420, 979, 1152, 1221
BstDEI CTNAG 3 cut(s) 147, 555, 973
BstDSI CCRYGG 1 cut(s) 485
BstF5I GGATG 5 cut(s) 226, 259, 298, 615, 668
BstFNI CGCG 2 cut(s) 104, 195
BstH2I RGCGCY 1 cut(s) 859
BstHHI GCGC 3 cut(s) 192, 197, 858
BstKTI GATC 6 cut(s) 153, 319, 796, 844, 1084, 1179
BstMAI GTCTC 1 cut(s) 472
BstMBI GATC 6 cut(s) 150, 316, 793, 841, 1081, 1176
BstMWI GCNNNNNNNGC 8 cut(s) 49, 263, 331, 379, 419, 424, 557, 978
BstNI CCWGG 3 cut(s) 271, 516, 596
BstSCI CCNGG 6 cut(s) 232, 237, 269, 336, 514, 594
BstSFI CTRYAG 1 cut(s) 1236
BstSLI GKGCMC 2 cut(s) 239, 667
BstUI CGCG 2 cut(s) 104, 195
BstV1I GCAGC 1 cut(s) 827
BstX2I RGATCY 3 cut(s) 316, 1081, 1176
BstYI RGATCY 3 cut(s) 316, 1081, 1176
Bsu15I ATCGAT 1 cut(s) 684
BsuI GTATCC 1 cut(s) 1092
BsuTUI ATCGAT 1 cut(s) 684
BtgI CCRYGG 1 cut(s) 485
BtgZI GCGATG 1 cut(s) 321
BtsCI GGATG 5 cut(s) 226, 259, 298, 615, 668
BtsIMutI CAGTG 1 cut(s) 1245
Cac8I GCNNGC 7 cut(s) 24, 45, 327, 420, 979, 1152, 1221
CaiI CAGNNNCTG 2 cut(s) 1127, 1150
CfoI GCGC 3 cut(s) 192, 197, 858
Cfr10I RCCGGY 1 cut(s) 445
Cfr13I GGNCC 6 cut(s) 230, 235, 236, 273, 509, 1109
ClaI ATCGAT 1 cut(s) 684
CseI GACGC 1 cut(s) 231
CsiI ACCWGGT 1 cut(s) 594
Csp6I GTAC 4 cut(s) 34, 94, 133, 184
CviAII CATG 6 cut(s) 97, 486, 1066, 1104, 1141, 1265
CviQI GTAC 4 cut(s) 34, 94, 133, 184
DdeI CTNAG 3 cut(s) 147, 555, 973
DinI GGCGCC 1 cut(s) 857
DpnI GATC 6 cut(s) 152, 318, 795, 843, 1083, 1178
DpnII GATC 6 cut(s) 150, 316, 793, 841, 1081, 1176
DraI TTTAAA 1 cut(s) 897
DrdI GACNNNNNNGTC 1 cut(s) 1259
DseDI GACNNNNNNGTC 1 cut(s) 1259
EaeI YGGCCR 3 cut(s) 264, 482, 537
EciI GGCGGA 2 cut(s) 575, 1173
Eco130I CCWWGG 5 cut(s) 78, 288, 485, 540, 1085
Eco147I AGGCCT 2 cut(s) 422, 1234
Eco24I GRGCYC 1 cut(s) 239
Eco47I GGWCC 1 cut(s) 1109
Eco57I CTGAAG 1 cut(s) 573
EcoRI GAATTC 1 cut(s) 349
EcoRII CCWGG 3 cut(s) 269, 514, 594
EcoT14I CCWWGG 5 cut(s) 78, 288, 485, 540, 1085
EcoT38I GRGCYC 1 cut(s) 239
EgeI GGCGCC 1 cut(s) 857
EheI GGCGCC 1 cut(s) 857
ErhI CCWWGG 5 cut(s) 78, 288, 485, 540, 1085
Esp3I CGTCTC 1 cut(s) 472
FaeI CATG 6 cut(s) 100, 489, 1069, 1107, 1144, 1268
FalI AAGNNNNNCTT 6 cut(s) 110, 142, 407, 439, 518, 550
FaqI GGGAC 1 cut(s) 721
FatI CATG 6 cut(s) 96, 485, 1065, 1103, 1140, 1264
FauI CCCGC 2 cut(s) 58, 465
FauNDI CATATG 2 cut(s) 832, 912
FbaI TGATCA 1 cut(s) 793
Fnu4HI GCNGC 5 cut(s) 193, 258, 304, 816, 1155
FokI GGATG 5 cut(s) 233, 266, 305, 622, 655
FriOI GRGCYC 1 cut(s) 239
Fsp4HI GCNGC 5 cut(s) 193, 258, 304, 816, 1155
FspBI CTAG 5 cut(s) 629, 741, 839, 929, 1086
GlaI GCGC 3 cut(s) 191, 196, 857
GluI GCNGC 5 cut(s) 193, 258, 304, 816, 1155
HaeII RGCGCY 1 cut(s) 859
HapII CCGG 5 cut(s) 233, 239, 320, 337, 446
HgaI GACGC 1 cut(s) 231
HhaI GCGC 3 cut(s) 192, 197, 858
Hin1I GRCGYC 3 cut(s) 242, 856, 1260
Hin1II CATG 6 cut(s) 100, 489, 1069, 1107, 1144, 1268
Hin6I GCGC 3 cut(s) 190, 195, 856
HinP1I GCGC 3 cut(s) 190, 195, 856
HindIII AAGCTT 2 cut(s) 735, 970
HinfI GANTC 5 cut(s) 205, 563, 695, 709, 1164
HpaII CCGG 5 cut(s) 233, 239, 320, 337, 446
HphI GGTGA 3 cut(s) 169, 626, 1129
Hpy166II GTNNAC 4 cut(s) 177, 665, 1109, 1275
Hpy188I TCNGA 4 cut(s) 592, 798, 1006, 1289
Hpy188III TCNNGA 4 cut(s) 118, 320, 656, 741
Hpy8I GTNNAC 4 cut(s) 177, 665, 1109, 1275
HpyAV CCTTC 4 cut(s) 293, 713, 1190, 1289
HpyCH4III ACNGT 1 cut(s) 1240
HpyCH4IV ACGT 2 cut(s) 36, 1260
HpyCH4V TGCA 9 cut(s) 52, 382, 427, 551, 665, 769, 775, 836, 1144
HpyF10VI GCNNNNNNNGC 8 cut(s) 49, 263, 331, 379, 419, 424, 557, 978
HpyF3I CTNAG 3 cut(s) 147, 555, 973
HpySE526I ACGT 2 cut(s) 36, 1260
Hsp92I GRCGYC 3 cut(s) 242, 856, 1260
Hsp92II CATG 6 cut(s) 100, 489, 1069, 1107, 1144, 1268
HspAI GCGC 3 cut(s) 190, 195, 856
KasI GGCGCC 1 cut(s) 855
Kpn2I TCCGGA 1 cut(s) 319
Ksp22I TGATCA 1 cut(s) 793
Kzo9I GATC 6 cut(s) 150, 316, 793, 841, 1081, 1176
LmnI GCTCC 4 cut(s) 19, 322, 339, 1147
Lsp1109I GCAGC 1 cut(s) 827
LweI GCATC 3 cut(s) 244, 762, 1051
MabI ACCWGGT 1 cut(s) 594
MaeI CTAG 5 cut(s) 629, 741, 839, 929, 1086
MaeII ACGT 2 cut(s) 36, 1260
MaeIII GTNAC 1 cut(s) 1250
MalI GATC 6 cut(s) 152, 318, 795, 843, 1083, 1178
MboI GATC 6 cut(s) 150, 316, 793, 841, 1081, 1176
MboII GAAGA 5 cut(s) 68, 151, 326, 576, 579
MflI RGATCY 3 cut(s) 316, 1081, 1176
MhlI GDGCHC 2 cut(s) 239, 667
MlsI TGGCCA 2 cut(s) 266, 539
MluCI AATT 6 cut(s) 10, 70, 162, 349, 452, 747
MluI ACGCGT 1 cut(s) 102
MluNI TGGCCA 2 cut(s) 266, 539
Mly113I GGCGCC 1 cut(s) 856
MlyI GAGTC 3 cut(s) 557, 703, 1158
MmeI TCCRAC 1 cut(s) 87
Mox20I TGGCCA 2 cut(s) 266, 539
MroI TCCGGA 1 cut(s) 319
MroXI GAANNNNTTC 1 cut(s) 1044
MscI TGGCCA 2 cut(s) 266, 539
MseI TTAA 4 cut(s) 750, 896, 950, 1023
Msp20I TGGCCA 2 cut(s) 266, 539
MspI CCGG 5 cut(s) 233, 239, 320, 337, 446
MspR9I CCNGG 6 cut(s) 234, 239, 271, 338, 516, 596
MteI GCGCNGCGC 1 cut(s) 193
MvaI CCWGG 3 cut(s) 271, 516, 596
MvnI CGCG 2 cut(s) 104, 195
MwoI GCNNNNNNNGC 8 cut(s) 49, 263, 331, 379, 419, 424, 557, 978
NarI GGCGCC 1 cut(s) 856
NciI CCSGG 3 cut(s) 234, 239, 338
NcoI CCATGG 1 cut(s) 485
NdeI CATATG 2 cut(s) 832, 912
NdeII GATC 6 cut(s) 150, 316, 793, 841, 1081, 1176
NlaIII CATG 6 cut(s) 100, 489, 1069, 1107, 1144, 1268
NlaIV GGNNCC 5 cut(s) 237, 787, 857, 1003, 1083
NmeAIII GCCGAG 1 cut(s) 223
NmuCI GTSAC 1 cut(s) 1250
PceI AGGCCT 2 cut(s) 422, 1234
PdmI GAANNNNTTC 1 cut(s) 1044
PfeI GAWTC 2 cut(s) 205, 695
PfoI TCCNGGA 1 cut(s) 514
PkrI GCNGC 5 cut(s) 194, 259, 305, 817, 1156
PleI GAGTC 3 cut(s) 557, 703, 1158
PluTI GGCGCC 1 cut(s) 859
PpsI GAGTC 3 cut(s) 557, 703, 1158
Ppu21I YACGTR 1 cut(s) 37
PsiI TTATAA 1 cut(s) 362
Psp6I CCWGG 3 cut(s) 269, 514, 594
PspGI CCWGG 3 cut(s) 269, 514, 594
PspN4I GGNNCC 5 cut(s) 237, 787, 857, 1003, 1083
PspOMI GGGCCC 1 cut(s) 235
PspPI GGNCC 6 cut(s) 230, 235, 236, 273, 509, 1109
PstNI CAGNNNCTG 2 cut(s) 1127, 1150
PsuI RGATCY 3 cut(s) 316, 1081, 1176
RsaI GTAC 4 cut(s) 35, 95, 134, 185
RsaNI GTAC 4 cut(s) 34, 94, 133, 184
SaqAI TTAA 4 cut(s) 750, 896, 950, 1023
SatI GCNGC 5 cut(s) 193, 258, 304, 816, 1155
Sau3AI GATC 6 cut(s) 150, 316, 793, 841, 1081, 1176
Sau96I GGNCC 6 cut(s) 230, 235, 236, 273, 509, 1109
ScaI AGTACT 1 cut(s) 185
SchI GAGTC 3 cut(s) 557, 703, 1158
ScrFI CCNGG 6 cut(s) 234, 239, 271, 338, 516, 596
SduI GDGCHC 2 cut(s) 239, 667
SexAI ACCWGGT 1 cut(s) 594
SfaNI GCATC 3 cut(s) 244, 762, 1051
SfcI CTRYAG 1 cut(s) 1236
SfoI GGCGCC 1 cut(s) 857
SinI GGWCC 1 cut(s) 1109
Sse9I AATT 6 cut(s) 10, 70, 162, 349, 452, 747
SseBI AGGCCT 2 cut(s) 422, 1234
SspDI GGCGCC 1 cut(s) 855
SspMI CTAG 5 cut(s) 629, 741, 839, 929, 1086
StuI AGGCCT 2 cut(s) 422, 1234
StyD4I CCNGG 6 cut(s) 232, 237, 269, 336, 514, 594
StyI CCWWGG 5 cut(s) 78, 288, 485, 540, 1085
TaaI ACNGT 1 cut(s) 1240
TaiI ACGT 2 cut(s) 39, 1263
TaqI TCGA 4 cut(s) 566, 684, 864, 1209
TasI AATT 6 cut(s) 10, 70, 162, 349, 452, 747
TatI WGTACW 2 cut(s) 93, 183
TauI GCSGC 4 cut(s) 195, 260, 306, 1157
TfiI GAWTC 2 cut(s) 205, 695
Tru1I TTAA 4 cut(s) 750, 896, 950, 1023
Tru9I TTAA 4 cut(s) 750, 896, 950, 1023
TscAI CASTG 1 cut(s) 1245
TseFI GTSAC 1 cut(s) 1250
TseI GCWGC 1 cut(s) 815
Tsp45I GTSAC 1 cut(s) 1250
TspDTI ATGAA 4 cut(s) 113, 670, 914, 981
TspGWI ACGGA 2 cut(s) 240, 770
TspRI CASTG 1 cut(s) 1245
VneI GTGCAC 1 cut(s) 663
VpaK11BI GGWCC 1 cut(s) 1109
XapI RAATTY 2 cut(s) 349, 452
XbaI TCTAGA 1 cut(s) 740
XcmI CCANNNNNNNNNTGG 1 cut(s) 1147
XmaJI CCTAGG 1 cut(s) 1085
XmnI GAANNNNTTC 1 cut(s) 1044
XspI CTAG 5 cut(s) 629, 741, 839, 929, 1086
ZraI GACGTC 1 cut(s) 1261
ZrmI AGTACT 1 cut(s) 185
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.