MD11G1161300.v1.1

Belongs to the AAA ATPase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0002427
Physical Location & Seq
Forward (+)
15938313 .. 15942789
4477 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1161300.v1.1.491

Sequence Viewer

Length: 1302 bp
ATGTATAGCAATTTCAAGGAGCAAGCGATTGAGTACGTGAAGCAAGCCGTGCAGGAAGATAATGCGGGAAATTACGCAAAGGCCTTCCCCTTGTACATGAACGCCTTGGAGTACTTCAAGACCCACTTGAAGTACGAGAAGAACCCTAAGATCAAGGAGGCAATAACCCAGAAGTTCACCGAGTACTTGCGCCGTGCCGAGGAGATTCGGGCGGTGTTGGATGACGGCGGCCCAGGGCCGGCGTCTAACGGGGATGCGGCGGTGGCCACCAAGCCCAAGACCAAGCCCAAAGACGGGGAGGGCGGTGACGGTGAGGATCCGGAGCAGGCGAAGCTCCGGGCGGGGCTGAATTCGGCGATTATAAGGGAAAAGCCCAATGTGCAATGGAATGATGTTGCTGGTTTGGAGAGTGCCAAGCAGGCCTTGCAAGAAGCGGTTATATTGCCGGTCAAATTCCCGCAGTTCTTTACTGGCAAGAGACGACCGTGGAGGGCATTTCTATTGTATGGGCCTCCTGGAACTGGAAAGTCTTACTTGGCCAAGGCTGTTGCAACCGAGGCAGACTCGACATTTTTCAGTATTTCGTCTTCAGACCTTGTTTCAAAATGGATGGGTGAGAGTGAAAAGCTAGTTTCAAACCTTTTCCAAATGGCTCGTGATAGTGCACCATCCATTATATTCATCGATGAGATAGATTCCTTATGTGGGACTCGTGGAGAAGGCAATGAGAGTGAAGCATCTAGACGAATTAAGACGGAACTTCTTGTGCAGATGCAGGGTGTGGGACACAATGATCAGAAAGTTCTTGTTCTTGCAGCAACAAATACTCCATATGCACTAGATCAGGCTATTCGGCGACGTTTTGACAAGCGTATATACATCCCCCTCCCAGATTTAAAGGCTCGACAGCATATGTTCAAAGTGCATCTAGGAGATACTCCCCACAACTTAACAGAAAGTGATTTCGAAAGCTTAGCTCGCAAAACTGATGGTTTTTCTGGTTCCGATATTGCTGTTTGTGTTAAAGACGTTCTCTTTGAACCTGTTCGTAAAACGCAAGATGCCATGTTCTTTTTCAAGGATCCTAAGGATATGTGGATACCATGTGGACCAAAGCAACCAGGTGCTGTCCAAATCACCATGCAGGATCTGGCGGCAAAAGGACTTGCGTCACAGATCCTTCCGCCTCCTATCACAAAAACAGATTTCGATAAGGTTCTTGCTAGACAGAGGCCTACAGTGAGCAAGAATGACCTTGAAGTCCATGAGAGATTCACAAGAGAGTTCGGAGAGGAAGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000902 GO:0000904 GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005770 GO:0005771 GO:0005911 GO:0006629 GO:0006810 GO:0006900 GO:0006914 GO:0006996 GO:0007032 GO:0007033 GO:0007034 GO:0008104 GO:0008144 GO:0008150 GO:0008152 GO:0008202 GO:0009056 GO:0009506 GO:0009653 GO:0009888 GO:0009987 GO:0010008 GO:0010026 GO:0010090 GO:0010091 GO:0010256 GO:0012505 GO:0016020 GO:0016043 GO:0016050 GO:0016125 GO:0016192 GO:0016197 GO:0016236 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017076 GO:0017111 GO:0022607 GO:0030054 GO:0030154 GO:0030554 GO:0031090 GO:0031410 GO:0031982 GO:0032502 GO:0032507 GO:0032509 GO:0032511 GO:0032553 GO:0032555 GO:0032559 GO:0032989 GO:0032991 GO:0033036 GO:0033365 GO:0034067 GO:0034613 GO:0035639 GO:0036094 GO:0036452 GO:0042592 GO:0042802 GO:0042803 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044422 GO:0044424 GO:0044425 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0045053 GO:0045185 GO:0045324 GO:0046907 GO:0046983 GO:0048468 GO:0048856 GO:0048869 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0051235 GO:0051259 GO:0051260 GO:0051641 GO:0051649 GO:0051651 GO:0055044 GO:0055065 GO:0055067 GO:0055075 GO:0055078 GO:0055080 GO:0061024 GO:0061919 GO:0065003 GO:0065007 GO:0065008 GO:0070676 GO:0070727 GO:0071704 GO:0071840 GO:0071985 GO:0090558 GO:0090626 GO:0097159 GO:0097367 GO:0097708 GO:0098588 GO:0098771 GO:0098796 GO:0098805 GO:1901265 GO:1901360 GO:1901363 GO:1901615 GO:1902494 GO:1904949 GO:1990621
KEGG Pathways
Metabolic & Signaling
ko04144 ko04217 map04144 map04217
Pfam Domains
Protein Families
AAA MIT Vps4_C

Protein Analysis

434

Amino Acids

48.33

Weight (kDa)

7.03

Isoelectric Point (pI)

40.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIT PF04212 7 - 70 6.8e-22 MIT (microtubule interacting and transport) domain
AAA PF00004 167 - 296 5e-44 ATPase family associated with various cellular activities (AAA)
AAA_lid_3 PF17862 321 - 354 4.3e-07 AAA+ lid domain
Vps4_C PF09336 366 - 430 7.7e-23 Vps4 C terminal oligomerisation domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 362
AasI GACNNNNNNGTC 1 cut(s) 1259
AccIII TCCGGA 1 cut(s) 319
AclWI GGATC 6 cut(s) 311, 324, 1076, 1089, 1155, 1171
AcoI YGGCCR 2 cut(s) 264, 537
AcsI RAATTY 2 cut(s) 349, 452
AcuI CTGAAG 1 cut(s) 573
AcyI GRCGYC 1 cut(s) 242
AfaI GTAC 5 cut(s) 35, 95, 113, 134, 185
AfiI CCNNNNNNNGG 6 cut(s) 199, 238, 293, 294, 521, 705
AgsI TTSAA 9 cut(s) 16, 118, 130, 603, 636, 919, 1040, 1078, 1259
AjnI CCWGG 3 cut(s) 232, 514, 1120
AluBI AGCT 4 cut(s) 334, 628, 972, 977
AluI AGCT 4 cut(s) 334, 628, 972, 977
Alw21I GWGCWC 1 cut(s) 667
Alw26I GTCTC 1 cut(s) 472
Alw44I GTGCAC 1 cut(s) 663
AlwI GGATC 6 cut(s) 311, 324, 1076, 1089, 1155, 1171
AlwNI CAGNNNCTG 2 cut(s) 1127, 1150
Aor13HI TCCGGA 1 cut(s) 319
AoxI GGCC 8 cut(s) 81, 229, 236, 264, 420, 509, 537, 1232
ApaLI GTGCAC 1 cut(s) 663
ApeKI GCWGC 1 cut(s) 815
ApoI RAATTY 2 cut(s) 349, 452
ArsI GACNNNNNNTTYG 2 cut(s) 1019, 1051
Asp700I GAANNNNTTC 1 cut(s) 1044
AspLEI GCGC 1 cut(s) 192
AspS9I GGNCC 4 cut(s) 230, 236, 509, 1109
AsuC2I CCSGG 1 cut(s) 338
AsuHPI GGTGA 5 cut(s) 169, 317, 323, 626, 1129
AsuII TTCGAA 1 cut(s) 966
AvaII GGWCC 1 cut(s) 1109
AxyI CCTNAGG 1 cut(s) 1086
BaeGI GKGCMC 1 cut(s) 667
BalI TGGCCA 2 cut(s) 266, 539
BamHI GGATCC 2 cut(s) 316, 1081
BarI GAAGNNNNNNTAC 2 cut(s) 571, 603
BauI CACGAG 2 cut(s) 654, 711
BbsI GAAGAC 1 cut(s) 579
Bbv12I GWGCWC 1 cut(s) 667
BbvI GCAGC 1 cut(s) 827
BccI CCATC 3 cut(s) 604, 676, 983
BceAI ACGGC 3 cut(s) 32, 177, 241
BciT130I CCWGG 3 cut(s) 234, 516, 1122
BciVI GTATCC 1 cut(s) 1092
BclI TGATCA 1 cut(s) 793
BcnI CCSGG 1 cut(s) 338
BcoDI GTCTC 1 cut(s) 472
BfaI CTAG 5 cut(s) 629, 741, 839, 929, 1224
BfmI CTRYAG 1 cut(s) 1236
BfuI GTATCC 1 cut(s) 1092
BglI GCCNNNNNGGC 1 cut(s) 419
BisI GCNGC 4 cut(s) 229, 258, 816, 1155
BlpI GCTNAGC 1 cut(s) 973
BlsI GCNGC 4 cut(s) 230, 259, 817, 1156
BmcAI AGTACT 2 cut(s) 113, 185
Bme1390I CCNGG 4 cut(s) 234, 338, 516, 1122
Bme18I GGWCC 1 cut(s) 1109
BmgT120I GGNCC 4 cut(s) 230, 236, 509, 1109
BmiI GGNNCC 3 cut(s) 318, 1003, 1083
BmrFI CCNGG 4 cut(s) 234, 338, 516, 1122
BmsI GCATC 5 cut(s) 244, 746, 762, 934, 1051
BoxI GACNNNNGTC 1 cut(s) 1168
BpiI GAAGAC 1 cut(s) 579
BplI GAGNNNNNCTC 2 cut(s) 548, 580
Bpu1102I GCTNAGC 1 cut(s) 973
Bpu14I TTCGAA 1 cut(s) 966
BpuMI CCSGG 1 cut(s) 338
Bsa29I ATCGAT 1 cut(s) 684
BsaAI YACGTR 1 cut(s) 37
BsaHI GRCGYC 1 cut(s) 242
BsaJI CCNNGG 7 cut(s) 105, 198, 232, 233, 485, 540, 555
BsaWI WCCGGW 1 cut(s) 319
BsaXI ACNNNNNCTCC 2 cut(s) 811, 841
Bsc4I CCNNNNNNNGG 6 cut(s) 199, 238, 293, 294, 521, 705
Bse118I RCCGGY 2 cut(s) 238, 445
Bse1I ACTGG 2 cut(s) 475, 526
Bse21I CCTNAGG 1 cut(s) 1086
Bse3DI GCAATG 2 cut(s) 389, 730
BseAI TCCGGA 1 cut(s) 319
BseBI CCWGG 3 cut(s) 234, 516, 1122
BseCI ATCGAT 1 cut(s) 684
BseDI CCNNGG 7 cut(s) 105, 198, 232, 233, 485, 540, 555
BseGI GGATG 5 cut(s) 226, 259, 615, 668, 879
BseLI CCNNNNNNNGG 6 cut(s) 199, 238, 293, 294, 521, 705
BseMI GCAATG 2 cut(s) 389, 730
BseNI ACTGG 2 cut(s) 475, 526
BseRI GAGGAG 1 cut(s) 215
BseSI GKGCMC 1 cut(s) 667
BseXI GCAGC 1 cut(s) 827
BsgI GTGCAG 2 cut(s) 71, 788
Bsh1285I CGRYCG 1 cut(s) 485
BshFI GGCC 8 cut(s) 83, 231, 238, 266, 422, 511, 539, 1234
BshVI ATCGAT 1 cut(s) 684
BsiEI CGRYCG 1 cut(s) 485
BsiHKAI GWGCWC 1 cut(s) 667
BsiSI CCGG 4 cut(s) 239, 320, 337, 446
BslFI GGGAC 2 cut(s) 721, 798
BslI CCNNNNNNNGG 6 cut(s) 199, 238, 293, 294, 521, 705
BsmAI GTCTC 1 cut(s) 472
BsmBI CGTCTC 1 cut(s) 472
BsmFI GGGAC 2 cut(s) 721, 798
BsnI GGCC 8 cut(s) 83, 231, 238, 266, 422, 511, 539, 1234
Bsp119I TTCGAA 1 cut(s) 966
Bsp1286I GDGCHC 1 cut(s) 667
Bsp13I TCCGGA 1 cut(s) 319
Bsp1407I TGTACA 1 cut(s) 93
Bsp143I GATC 7 cut(s) 150, 316, 793, 841, 1081, 1147, 1176
Bsp1720I GCTNAGC 1 cut(s) 973
BspANI GGCC 8 cut(s) 83, 231, 238, 266, 422, 511, 539, 1234
BspDI ATCGAT 1 cut(s) 684
BspEI TCCGGA 1 cut(s) 319
BspLI GGNNCC 3 cut(s) 318, 1003, 1083
BspPI GGATC 6 cut(s) 311, 324, 1076, 1089, 1155, 1171
BspT104I TTCGAA 1 cut(s) 966
BsrDI GCAATG 2 cut(s) 389, 730
BsrFI RCCGGY 2 cut(s) 238, 445
BsrGI TGTACA 1 cut(s) 93
BsrI ACTGG 2 cut(s) 475, 526
BssAI RCCGGY 2 cut(s) 238, 445
BssECI CCNNGG 7 cut(s) 105, 198, 232, 233, 485, 540, 555
BssMI GATC 7 cut(s) 150, 316, 793, 841, 1081, 1147, 1176
BssNI GRCGYC 1 cut(s) 242
BssSI CACGAG 2 cut(s) 654, 711
BssT1I CCWWGG 2 cut(s) 105, 540
Bst2BI CACGAG 2 cut(s) 654, 711
Bst2UI CCWGG 3 cut(s) 234, 516, 1122
Bst4CI ACNGT 3 cut(s) 311, 486, 1240
BstACI GRCGYC 1 cut(s) 242
BstAPI GCANNNNNTGC 2 cut(s) 49, 424
BstAUI TGTACA 1 cut(s) 93
BstBAI YACGTR 1 cut(s) 37
BstBI TTCGAA 1 cut(s) 966
BstC8I GCNNGC 6 cut(s) 24, 45, 240, 327, 420, 979
BstDEI CTNAG 3 cut(s) 147, 973, 1086
BstDSI CCRYGG 1 cut(s) 485
BstF5I GGATG 5 cut(s) 226, 259, 615, 668, 879
BstHHI GCGC 1 cut(s) 192
BstKTI GATC 7 cut(s) 153, 319, 796, 844, 1084, 1150, 1179
BstMAI GTCTC 1 cut(s) 472
BstMBI GATC 7 cut(s) 150, 316, 793, 841, 1081, 1147, 1176
BstMCI CGRYCG 1 cut(s) 485
BstMWI GCNNNNNNNGC 8 cut(s) 49, 263, 331, 379, 419, 424, 557, 978
BstNI CCWGG 3 cut(s) 234, 516, 1122
BstPAI GACNNNNGTC 1 cut(s) 1168
BstSCI CCNGG 4 cut(s) 232, 336, 514, 1120
BstSFI CTRYAG 1 cut(s) 1236
BstSLI GKGCMC 1 cut(s) 667
BstV1I GCAGC 1 cut(s) 827
BstV2I GAAGAC 1 cut(s) 579
BstX2I RGATCY 4 cut(s) 316, 1081, 1147, 1176
BstYI RGATCY 4 cut(s) 316, 1081, 1147, 1176
Bsu15I ATCGAT 1 cut(s) 684
Bsu36I CCTNAGG 1 cut(s) 1086
BsuI GTATCC 1 cut(s) 1092
BsuRI GGCC 8 cut(s) 83, 231, 238, 266, 422, 511, 539, 1234
BsuTUI ATCGAT 1 cut(s) 684
BtgI CCRYGG 1 cut(s) 485
BtsCI GGATG 5 cut(s) 226, 259, 615, 668, 879
BtsIMutI CAGTG 1 cut(s) 1245
Cac8I GCNNGC 6 cut(s) 24, 45, 240, 327, 420, 979
CaiI CAGNNNCTG 2 cut(s) 1127, 1150
CfoI GCGC 1 cut(s) 192
Cfr10I RCCGGY 2 cut(s) 238, 445
Cfr13I GGNCC 4 cut(s) 230, 236, 509, 1109
ClaI ATCGAT 1 cut(s) 684
CseI GACGC 2 cut(s) 231, 1158
CsiI ACCWGGT 1 cut(s) 1120
Csp6I GTAC 5 cut(s) 34, 94, 112, 133, 184
CviAII CATG 5 cut(s) 97, 1066, 1104, 1141, 1265
CviQI GTAC 5 cut(s) 34, 94, 112, 133, 184
DdeI CTNAG 3 cut(s) 147, 973, 1086
DpnI GATC 7 cut(s) 152, 318, 795, 843, 1083, 1149, 1178
DpnII GATC 7 cut(s) 150, 316, 793, 841, 1081, 1147, 1176
DraI TTTAAA 1 cut(s) 897
DrdI GACNNNNNNGTC 1 cut(s) 1259
DseDI GACNNNNNNGTC 1 cut(s) 1259
EaeI YGGCCR 2 cut(s) 264, 537
EciI GGCGGA 1 cut(s) 1173
Eco130I CCWWGG 2 cut(s) 105, 540
Eco147I AGGCCT 3 cut(s) 83, 422, 1234
Eco47I GGWCC 1 cut(s) 1109
Eco57I CTGAAG 1 cut(s) 573
Eco81I CCTNAGG 1 cut(s) 1086
EcoRI GAATTC 1 cut(s) 349
EcoRII CCWGG 3 cut(s) 232, 514, 1120
EcoT14I CCWWGG 2 cut(s) 105, 540
ErhI CCWWGG 2 cut(s) 105, 540
Esp3I CGTCTC 1 cut(s) 472
FaeI CATG 5 cut(s) 100, 1069, 1107, 1144, 1268
FalI AAGNNNNNCTT 6 cut(s) 110, 142, 407, 439, 518, 550
FaqI GGGAC 2 cut(s) 721, 798
FatI CATG 5 cut(s) 96, 1065, 1103, 1140, 1264
FauI CCCGC 3 cut(s) 58, 334, 465
FauNDI CATATG 2 cut(s) 832, 912
FbaI TGATCA 1 cut(s) 793
Fnu4HI GCNGC 4 cut(s) 229, 258, 816, 1155
FokI GGATG 5 cut(s) 233, 266, 622, 655, 866
Fsp4HI GCNGC 4 cut(s) 229, 258, 816, 1155
FspBI CTAG 5 cut(s) 629, 741, 839, 929, 1224
GlaI GCGC 1 cut(s) 191
GluI GCNGC 4 cut(s) 229, 258, 816, 1155
HaeIII GGCC 8 cut(s) 83, 231, 238, 266, 422, 511, 539, 1234
HapII CCGG 4 cut(s) 239, 320, 337, 446
HgaI GACGC 2 cut(s) 231, 1158
HhaI GCGC 1 cut(s) 192
Hin1I GRCGYC 1 cut(s) 242
Hin1II CATG 5 cut(s) 100, 1069, 1107, 1144, 1268
Hin6I GCGC 1 cut(s) 190
HinP1I GCGC 1 cut(s) 190
HindIII AAGCTT 1 cut(s) 970
HinfI GANTC 5 cut(s) 205, 563, 695, 709, 1272
HpaII CCGG 4 cut(s) 239, 320, 337, 446
HphI GGTGA 5 cut(s) 169, 317, 323, 626, 1129
Hpy166II GTNNAC 3 cut(s) 177, 665, 1109
Hpy188I TCNGA 4 cut(s) 592, 798, 1006, 1289
Hpy188III TCNNGA 4 cut(s) 118, 320, 656, 741
Hpy8I GTNNAC 3 cut(s) 177, 665, 1109
Hpy99I CGWCG 1 cut(s) 861
HpyAV CCTTC 4 cut(s) 94, 713, 1190, 1289
HpyCH4III ACNGT 3 cut(s) 311, 486, 1240
HpyCH4IV ACGT 3 cut(s) 36, 859, 1029
HpyF10VI GCNNNNNNNGC 8 cut(s) 49, 263, 331, 379, 419, 424, 557, 978
HpyF3I CTNAG 3 cut(s) 147, 973, 1086
HpySE526I ACGT 3 cut(s) 36, 859, 1029
Hsp92I GRCGYC 1 cut(s) 242
Hsp92II CATG 5 cut(s) 100, 1069, 1107, 1144, 1268
HspAI GCGC 1 cut(s) 190
Kpn2I TCCGGA 1 cut(s) 319
KroI GCCGGC 1 cut(s) 238
KroNI GCCGGC 1 cut(s) 240
Ksp22I TGATCA 1 cut(s) 793
Kzo9I GATC 7 cut(s) 150, 316, 793, 841, 1081, 1147, 1176
LmnI GCTCC 3 cut(s) 19, 322, 339
Lsp1109I GCAGC 1 cut(s) 827
LweI GCATC 5 cut(s) 244, 746, 762, 934, 1051
MabI ACCWGGT 1 cut(s) 1120
MaeI CTAG 5 cut(s) 629, 741, 839, 929, 1224
MaeII ACGT 3 cut(s) 36, 859, 1029
MaeIII GTNAC 2 cut(s) 305, 1170
MalI GATC 7 cut(s) 152, 318, 795, 843, 1083, 1149, 1178
MboI GATC 7 cut(s) 150, 316, 793, 841, 1081, 1147, 1176
MboII GAAGA 3 cut(s) 68, 151, 579
MflI RGATCY 4 cut(s) 316, 1081, 1147, 1176
MhlI GDGCHC 1 cut(s) 667
MlsI TGGCCA 2 cut(s) 266, 539
MluCI AATT 5 cut(s) 10, 70, 349, 452, 747
MluNI TGGCCA 2 cut(s) 266, 539
MlyI GAGTC 2 cut(s) 557, 703
MmeI TCCRAC 1 cut(s) 198
Mox20I TGGCCA 2 cut(s) 266, 539
MroI TCCGGA 1 cut(s) 319
MroNI GCCGGC 1 cut(s) 238
MroXI GAANNNNTTC 1 cut(s) 1044
MscI TGGCCA 2 cut(s) 266, 539
MseI TTAA 4 cut(s) 750, 896, 950, 1023
Msp20I TGGCCA 2 cut(s) 266, 539
MspI CCGG 4 cut(s) 239, 320, 337, 446
MspR9I CCNGG 4 cut(s) 234, 338, 516, 1122
MvaI CCWGG 3 cut(s) 234, 516, 1122
MwoI GCNNNNNNNGC 8 cut(s) 49, 263, 331, 379, 419, 424, 557, 978
NaeI GCCGGC 1 cut(s) 240
NciI CCSGG 1 cut(s) 338
NdeI CATATG 2 cut(s) 832, 912
NdeII GATC 7 cut(s) 150, 316, 793, 841, 1081, 1147, 1176
NgoMIV GCCGGC 1 cut(s) 238
NlaIII CATG 5 cut(s) 100, 1069, 1107, 1144, 1268
NlaIV GGNNCC 3 cut(s) 318, 1003, 1083
NmeAIII GCCGAG 1 cut(s) 223
NmuCI GTSAC 2 cut(s) 305, 1170
NspV TTCGAA 1 cut(s) 966
PasI CCCWGGG 1 cut(s) 233
PceI AGGCCT 3 cut(s) 83, 422, 1234
PdiI GCCGGC 1 cut(s) 240
PdmI GAANNNNTTC 1 cut(s) 1044
PfeI GAWTC 3 cut(s) 205, 695, 1272
PfoI TCCNGGA 1 cut(s) 514
PkrI GCNGC 4 cut(s) 230, 259, 817, 1156
PleI GAGTC 2 cut(s) 557, 703
PpsI GAGTC 2 cut(s) 557, 703
Ppu21I YACGTR 1 cut(s) 37
PshAI GACNNNNGTC 1 cut(s) 1168
PsiI TTATAA 1 cut(s) 362
Psp6I CCWGG 3 cut(s) 232, 514, 1120
PspGI CCWGG 3 cut(s) 232, 514, 1120
PspN4I GGNNCC 3 cut(s) 318, 1003, 1083
PspPI GGNCC 4 cut(s) 230, 236, 509, 1109
PstNI CAGNNNCTG 2 cut(s) 1127, 1150
PsuI RGATCY 4 cut(s) 316, 1081, 1147, 1176
RsaI GTAC 5 cut(s) 35, 95, 113, 134, 185
RsaNI GTAC 5 cut(s) 34, 94, 112, 133, 184
SaqAI TTAA 4 cut(s) 750, 896, 950, 1023
SatI GCNGC 4 cut(s) 229, 258, 816, 1155
Sau3AI GATC 7 cut(s) 150, 316, 793, 841, 1081, 1147, 1176
Sau96I GGNCC 4 cut(s) 230, 236, 509, 1109
ScaI AGTACT 2 cut(s) 113, 185
SchI GAGTC 2 cut(s) 557, 703
ScrFI CCNGG 4 cut(s) 234, 338, 516, 1122
SduI GDGCHC 1 cut(s) 667
SexAI ACCWGGT 1 cut(s) 1120
SfaNI GCATC 5 cut(s) 244, 746, 762, 934, 1051
SfcI CTRYAG 1 cut(s) 1236
SfuI TTCGAA 1 cut(s) 966
SinI GGWCC 1 cut(s) 1109
Sse9I AATT 5 cut(s) 10, 70, 349, 452, 747
SseBI AGGCCT 3 cut(s) 83, 422, 1234
SspMI CTAG 5 cut(s) 629, 741, 839, 929, 1224
StuI AGGCCT 3 cut(s) 83, 422, 1234
StyD4I CCNGG 4 cut(s) 232, 336, 514, 1120
StyI CCWWGG 2 cut(s) 105, 540
TaaI ACNGT 3 cut(s) 311, 486, 1240
TaiI ACGT 3 cut(s) 39, 862, 1032
TaqI TCGA 5 cut(s) 566, 684, 904, 966, 1209
TasI AATT 5 cut(s) 10, 70, 349, 452, 747
TatI WGTACW 3 cut(s) 93, 111, 183
TauI GCSGC 3 cut(s) 231, 260, 1157
TfiI GAWTC 3 cut(s) 205, 695, 1272
Tru1I TTAA 4 cut(s) 750, 896, 950, 1023
Tru9I TTAA 4 cut(s) 750, 896, 950, 1023
TscAI CASTG 1 cut(s) 1245
TseFI GTSAC 2 cut(s) 305, 1170
TseI GCWGC 1 cut(s) 815
Tsp45I GTSAC 2 cut(s) 305, 1170
TspDTI ATGAA 2 cut(s) 113, 670
TspGWI ACGGA 1 cut(s) 770
TspRI CASTG 1 cut(s) 1245
VneI GTGCAC 1 cut(s) 663
VpaK11BI GGWCC 1 cut(s) 1109
XapI RAATTY 2 cut(s) 349, 452
XbaI TCTAGA 1 cut(s) 740
XcmI CCANNNNNNNNNTGG 1 cut(s) 1147
XmnI GAANNNNTTC 1 cut(s) 1044
XspI CTAG 5 cut(s) 629, 741, 839, 929, 1224
ZrmI AGTACT 2 cut(s) 113, 185
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.