Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0001633

Physical Location & Seq

Genomic Coordinates Reverse (-)

24547995 .. 24550824

Gene Structure 2830 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1178500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1155 bp

ATGGCTGATAGGCGCCAGCACCCAACTATCACGGAAAAGTTTTCTGGTCATGAGCTACATCTGAGTTCCAGCCACTATGGGGTCATCCAGAGGCCTTCTATCAATCAAAGGCGTTTTTCATATGGAAGTTATTCAAATGCAGCATTGCAATATCCCATGAACCAAGCATGTCGAGCTAATGATCCGTTGGTGGCTTCACGTTCCTTGCCTGTCTGTGTCGAAGCCCCGAAGGAGAAAGGATTTTCAGGCTTTGCAATTGATTTTCTAATGGGTGGAGTTTCTGCTGCTGTATCCAAGACTGCTGCTGCTCCAATTGAGCGCGTAAAGCTCTTAATTCAAAATCAGGATGAGATGATCAAGGCTGGCAGGCTCTCTGAACCCTACAAGGGAATTGGAGATTGTTTTAGCCGAACAATGAAAGATGAGGGAATGGTAGCATTGTGGAGAGGAAACACGGCAAATGTCATCCGTTACTTCCCAACTCAGGCCTTAAACTTCGCGTTTAAAGATTACTTCAAGAGCCTGTTCAATTTCAGAAAGGACAAGGATGGCTATTGGAAATGGTTTGCCGGTAACTTGGCATCTGGAGGTGCTGCGGGTGCCTCTTCTCTATTCTTTGTCTACTCTTTGGATTATGCTCGTACCCGTCTTGCAAATGATTCCAAGGCTGCAAAGAAGGGAGGAGAGAGGCAATTCAATGGCCTGGTTGATGTCTACAAAAAGACTCTCAAATCAGATGGCATTGCTGGCCTCTACCGCGGCTTCAACATTTCTTGTGTCGGAATCATTGTTTACCGTGGTCTTTACTTTGGAATGTATGATTCTTTGAAGCCTGTGATCCTCACTGGAAAGTTGCAGGATAGTTTCTTTGCTAGCTTTGGCCTTGGTTGGGTTATCACCAACGGCGCTGGACTTGCATCTTACCCAATTGACACTGTCCGTAGAAGGATGATGATGACATCTGGTGAAGCCGTCAAGTACAAGAGCTCGCTCGACGCTTTCTCTCAAATCCTTAAGAATGAGGGTGCGAGGTCTCTCTTCAAGGGTGCTGGTGCTAACATTCTTCGTGCAGTTGCTGGCGCGGGTGTGCTTGCAGGTTACGACAAGCTTCAGGTGCTAGTGTTCGGAAAGAAGTATGGTTCTGGTGGAGCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0016020 GO:0019866 GO:0031090 GO:0031966 GO:0031967 GO:0031975 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko04020 ko04022 ko04217 ko04218 ko05012 ko05016 ko05166 map04020 map04022 map04217 map04218 map05012 map05016 map05166

Pfam Domains

Protein Families

Mito_carr

Protein Analysis

385

Amino Acids

42.01

Weight (kDa)

9.77

Isoelectric Point (pI)

29.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Mito_carr	PF00153	84 - 178	6e-25	Mitochondrial carrier protein
Mito_carr	PF00153	187 - 281	3.4e-21	Mitochondrial carrier protein
Mito_carr	PF00153	287 - 376	3.3e-17	Mitochondrial carrier protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001633)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G08580 AT3G08580 AT5G13490 AT5G13490
fragaria_vesca	FvH4_3g29080 FvH4_3g29080 FvH4_3g29080 FvH4_7g00912 FvH4_7g00912 FvH4_7g00920
malus_domestica	MD00G1204500.v1.1 MD03G1178500.v1.1 MD07G1008900.v1.1 MD07G1009000.v1.1 MD07G1009200.v1.1 MD11G1198100.v1.1
prunus_persica	Prupe.2G012200_v2.0.a1 Prupe.2G012200_v2.0.a1 Prupe.2G012200_v2.0.a1 Prupe.4G244400_v2.0.a1 Prupe.4G244400_v2.0.a1
pyrus_communis	pycom03g13500 pycom07g00770
rosa_chinensis	RchiOBHm_Chr1g0315841 RchiOBHm_Chr1g0315851 RchiOBHm_Chr5g0051451
rosa_laevigata	RLG00000030729 RLG00000034875
rosa_multiflora	Rmu_co8322969.1_g000001 Rmu_sc0022189.1_g000005 Rmu_sc0023933.1_g000005 Rmu_ssc0000154.1_g000011 Rmu_ssc0000154.1_g000012
rosa_roxburghii	Rroxscaffold_1G00027060 Rroxscaffold_4G00331380 Rroxscaffold_4G00331400
rosa_rugosa	Rorug01G0006100 Rorug01G0006100 Rorug01G0006200 Rorug05G0279300 Rorug05G0279400 Rorug05G0279500 Rorug05G0279600
rosa_samantha	Rh1AG017100 Rh1AG017200 Rh5CG387300
rosa_wichuraiana	Rw1G001010 Rw1G001020 Rw5G033150

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1085
AccB1I	GGYRCC	2 cut(s)	12, 599
AccI	GTMKAC	2 cut(s)	621, 714
AccII	CGCG	4 cut(s)	321, 500, 759, 1082
AciI	CCGC	4 cut(s)	596, 757, 759, 1082
AclWI	GGATC	2 cut(s)	176, 832
AcuI	CTGAAG	1 cut(s)	1094
AcyI	GRCGYC	1 cut(s)	13
AfaI	GTAC	2 cut(s)	643, 980
AfiI	CCNNNNNNNGG	4 cut(s)	79, 386, 484, 889
AflII	CTTAAG	1 cut(s)	1013
AgsI	TTSAA	8 cut(s)	135, 338, 517, 529, 697, 766, 829, 1042
AjnI	CCWGG	1 cut(s)	702
AluBI	AGCT	7 cut(s)	55, 176, 328, 876, 987, 1108, 1151
AluI	AGCT	7 cut(s)	55, 176, 328, 876, 987, 1108, 1151
Alw21I	GWGCWC	1 cut(s)	989
Alw26I	GTCTC	1 cut(s)	1038
AlwI	GGATC	2 cut(s)	176, 832
AlwNI	CAGNNNCTG	1 cut(s)	1076
AoxI	GGCC	5 cut(s)	92, 486, 700, 748, 880
ApeKI	GCWGC	6 cut(s)	140, 284, 302, 305, 593, 668
Asp700I	GAANNNNTTC	1 cut(s)	130
AspLEI	GCGC	4 cut(s)	15, 321, 908, 1082
AsuHPI	GGTGA	2 cut(s)	889, 977
AsuNHI	GCTAGC	1 cut(s)	872
BanI	GGYRCC	2 cut(s)	12, 599
BanII	GRGCYC	1 cut(s)	989
Bbv12I	GWGCWC	1 cut(s)	989
BbvI	GCAGC	6 cut(s)	152, 271, 289, 292, 580, 655
BccI	CCATC	2 cut(s)	542, 731
BceAI	ACGGC	3 cut(s)	471, 919, 956
BciT130I	CCWGG	1 cut(s)	704
BciVI	GTATCC	1 cut(s)	301
BclI	TGATCA	1 cut(s)	354
BcoDI	GTCTC	1 cut(s)	1038
BfaI	CTAG	2 cut(s)	873, 1118
BfoI	RGCGCY	2 cut(s)	16, 909
BfrI	CTTAAG	1 cut(s)	1013
BfuAI	ACCTGC	1 cut(s)	1085
BfuI	GTATCC	1 cut(s)	301
BisI	GCNGC	7 cut(s)	141, 285, 303, 306, 594, 669, 760
BlsI	GCNGC	7 cut(s)	142, 286, 304, 307, 595, 670, 761
Bme1390I	CCNGG	1 cut(s)	704
BmiI	GGNNCC	2 cut(s)	14, 601
BmrFI	CCNGG	1 cut(s)	704
BmsI	GCATC	2 cut(s)	590, 926
BmtI	GCTAGC	1 cut(s)	876
BpmI	CTGGAG	1 cut(s)	606
BsaHI	GRCGYC	1 cut(s)	13
BsaI	GGTCTC	1 cut(s)	1038
BsaJI	CCNNGG	4 cut(s)	663, 757, 796, 883
Bsc4I	CCNNNNNNNGG	4 cut(s)	79, 386, 484, 889
Bse118I	RCCGGY	1 cut(s)	569
Bse1I	ACTGG	1 cut(s)	850
Bse3DI	GCAATG	2 cut(s)	143, 741
BseBI	CCWGG	1 cut(s)	704
BseDI	CCNNGG	4 cut(s)	663, 757, 796, 883
BseGI	GGATG	5 cut(s)	84, 352, 465, 553, 954
BseLI	CCNNNNNNNGG	4 cut(s)	79, 386, 484, 889
BseMI	GCAATG	2 cut(s)	143, 741
BseMII	CTCAG	2 cut(s)	53, 497
BseNI	ACTGG	1 cut(s)	850
BseRI	GAGGAG	1 cut(s)	696
BseXI	GCAGC	6 cut(s)	152, 271, 289, 292, 580, 655
BsgI	GTGCAG	1 cut(s)	1089
Bsh1236I	CGCG	4 cut(s)	321, 500, 759, 1082
BshFI	GGCC	5 cut(s)	94, 488, 702, 750, 882
BshNI	GGYRCC	2 cut(s)	12, 599
BsiHKAI	GWGCWC	1 cut(s)	989
BsiSI	CCGG	1 cut(s)	570
BslI	CCNNNNNNNGG	4 cut(s)	79, 386, 484, 889
BsmAI	GTCTC	1 cut(s)	1038
BsnI	GGCC	5 cut(s)	94, 488, 702, 750, 882
Bso31I	GGTCTC	1 cut(s)	1038
Bsp1286I	GDGCHC	1 cut(s)	989
Bsp143I	GATC	3 cut(s)	181, 354, 837
BspACI	CCGC	4 cut(s)	596, 757, 759, 1082
BspANI	GGCC	5 cut(s)	94, 488, 702, 750, 882
BspCNI	CTCAG	2 cut(s)	54, 496
BspFNI	CGCG	4 cut(s)	321, 500, 759, 1082
BspHI	TCATGA	1 cut(s)	49
BspLI	GGNNCC	2 cut(s)	14, 601
BspMI	ACCTGC	1 cut(s)	1085
BspOI	GCTAGC	1 cut(s)	876
BspPI	GGATC	2 cut(s)	176, 832
BspT107I	GGYRCC	2 cut(s)	12, 599
BspTI	CTTAAG	1 cut(s)	1013
BspTNI	GGTCTC	1 cut(s)	1038
BsrDI	GCAATG	2 cut(s)	143, 741
BsrFI	RCCGGY	1 cut(s)	569
BsrI	ACTGG	1 cut(s)	850
BssAI	RCCGGY	1 cut(s)	569
BssECI	CCNNGG	4 cut(s)	663, 757, 796, 883
BssMI	GATC	3 cut(s)	181, 354, 837
BssNI	GRCGYC	1 cut(s)	13
BssT1I	CCWWGG	2 cut(s)	663, 883
Bst2UI	CCWGG	1 cut(s)	704
Bst4CI	ACNGT	2 cut(s)	797, 937
Bst6I	CTCTTC	2 cut(s)	610, 1043
BstACI	GRCGYC	1 cut(s)	13
BstAFI	CTTAAG	1 cut(s)	1013
BstC8I	GCNNGC	8 cut(s)	17, 364, 368, 748, 874, 989, 1078, 1092
BstDEI	CTNAG	2 cut(s)	62, 483
BstDSI	CCRYGG	2 cut(s)	757, 796
BstF5I	GGATG	5 cut(s)	84, 352, 465, 553, 954
BstFNI	CGCG	4 cut(s)	321, 500, 759, 1082
BstH2I	RGCGCY	2 cut(s)	16, 909
BstHHI	GCGC	4 cut(s)	15, 321, 908, 1082
BstKTI	GATC	3 cut(s)	184, 357, 840
BstMAI	GTCTC	1 cut(s)	1038
BstMBI	GATC	3 cut(s)	181, 354, 837
BstMWI	GCNNNNNNNGC	7 cut(s)	173, 325, 599, 747, 756, 914, 1114
BstNI	CCWGG	1 cut(s)	704
BstNSI	RCATGY	1 cut(s)	171
BstSCI	CCNGG	1 cut(s)	702
BstUI	CGCG	4 cut(s)	321, 500, 759, 1082
BstV1I	GCAGC	6 cut(s)	152, 271, 289, 292, 580, 655
BsuI	GTATCC	1 cut(s)	301
BsuRI	GGCC	5 cut(s)	94, 488, 702, 750, 882
BtgI	CCRYGG	2 cut(s)	757, 796
BtsCI	GGATG	5 cut(s)	84, 352, 465, 553, 954
BtsIMutI	CAGTG	2 cut(s)	843, 933
BveI	ACCTGC	1 cut(s)	1085
Cac8I	GCNNGC	8 cut(s)	17, 364, 368, 748, 874, 989, 1078, 1092
CaiI	CAGNNNCTG	1 cut(s)	1076
CciI	TCATGA	1 cut(s)	49
CfoI	GCGC	4 cut(s)	15, 321, 908, 1082
Cfr10I	RCCGGY	1 cut(s)	569
Cfr42I	CCGCGG	1 cut(s)	760
CseI	GACGC	1 cut(s)	1004
Csp6I	GTAC	2 cut(s)	642, 979
CviAII	CATG	3 cut(s)	50, 157, 168
CviQI	GTAC	2 cut(s)	642, 979
DdeI	CTNAG	2 cut(s)	62, 483
DinI	GGCGCC	1 cut(s)	14
DpnI	GATC	3 cut(s)	183, 356, 839
DpnII	GATC	3 cut(s)	181, 354, 837
DraI	TTTAAA	1 cut(s)	505
Eam1104I	CTCTTC	2 cut(s)	610, 1043
EarI	CTCTTC	2 cut(s)	610, 1043
Ecl136II	GAGCTC	1 cut(s)	987
Eco130I	CCWWGG	2 cut(s)	663, 883
Eco147I	AGGCCT	2 cut(s)	94, 488
Eco24I	GRGCYC	1 cut(s)	989
Eco31I	GGTCTC	1 cut(s)	1038
Eco53kI	GAGCTC	1 cut(s)	987
Eco57I	CTGAAG	1 cut(s)	1094
EcoICRI	GAGCTC	1 cut(s)	987
EcoRII	CCWGG	1 cut(s)	702
EcoT14I	CCWWGG	2 cut(s)	663, 883
EcoT38I	GRGCYC	1 cut(s)	989
EgeI	GGCGCC	1 cut(s)	14
EheI	GGCGCC	1 cut(s)	14
ErhI	CCWWGG	2 cut(s)	663, 883
FaeI	CATG	3 cut(s)	53, 160, 171
FaiI	YATR	9 cut(s)	51, 78, 121, 123, 158, 169, 636, 819, 1137
FatI	CATG	3 cut(s)	49, 156, 167
FauI	CCCGC	2 cut(s)	589, 1075
FauNDI	CATATG	1 cut(s)	121
FbaI	TGATCA	1 cut(s)	354
FblI	GTMKAC	2 cut(s)	621, 714
Fnu4HI	GCNGC	7 cut(s)	141, 285, 303, 306, 594, 669, 760
FokI	GGATG	5 cut(s)	71, 359, 452, 560, 961
FriOI	GRGCYC	1 cut(s)	989
Fsp4HI	GCNGC	7 cut(s)	141, 285, 303, 306, 594, 669, 760
FspBI	CTAG	2 cut(s)	873, 1118
GlaI	GCGC	4 cut(s)	14, 320, 907, 1081
GluI	GCNGC	7 cut(s)	141, 285, 303, 306, 594, 669, 760
GsuI	CTGGAG	1 cut(s)	606
HaeII	RGCGCY	2 cut(s)	16, 909
HaeIII	GGCC	5 cut(s)	94, 488, 702, 750, 882
HapII	CCGG	1 cut(s)	570
HgaI	GACGC	1 cut(s)	1004
HhaI	GCGC	4 cut(s)	15, 321, 908, 1082
Hin1I	GRCGYC	1 cut(s)	13
Hin1II	CATG	3 cut(s)	53, 160, 171
Hin6I	GCGC	4 cut(s)	13, 319, 906, 1080
HinP1I	GCGC	4 cut(s)	13, 319, 906, 1080
HindIII	AAGCTT	1 cut(s)	1106
HinfI	GANTC	4 cut(s)	659, 724, 783, 821
HpaII	CCGG	1 cut(s)	570
HphI	GGTGA	2 cut(s)	889, 977
Hpy166II	GTNNAC	3 cut(s)	622, 715, 793
Hpy188I	TCNGA	6 cut(s)	63, 376, 536, 736, 782, 1127
Hpy188III	TCNNGA	5 cut(s)	50, 88, 344, 517, 585
Hpy8I	GTNNAC	3 cut(s)	622, 715, 793
Hpy99I	CGWCG	1 cut(s)	998
HpyAV	CCTTC	4 cut(s)	105, 223, 670, 939
HpyCH4III	ACNGT	2 cut(s)	797, 937
HpyCH4IV	ACGT	1 cut(s)	199
HpyCH4V	TGCA	9 cut(s)	140, 148, 254, 653, 671, 856, 917, 1070, 1094
HpyF10VI	GCNNNNNNNGC	7 cut(s)	173, 325, 599, 747, 756, 914, 1114
HpyF3I	CTNAG	2 cut(s)	62, 483
HpySE526I	ACGT	1 cut(s)	199
Hsp92I	GRCGYC	1 cut(s)	13
Hsp92II	CATG	3 cut(s)	53, 160, 171
HspAI	GCGC	4 cut(s)	13, 319, 906, 1080
KasI	GGCGCC	1 cut(s)	12
Ksp22I	TGATCA	1 cut(s)	354
KspI	CCGCGG	1 cut(s)	760
Kzo9I	GATC	3 cut(s)	181, 354, 837
LmnI	GCTCC	2 cut(s)	313, 1148
Lsp1109I	GCAGC	6 cut(s)	152, 271, 289, 292, 580, 655
LweI	GCATC	2 cut(s)	590, 926
MaeI	CTAG	2 cut(s)	873, 1118
MaeII	ACGT	1 cut(s)	199
MaeIII	GTNAC	3 cut(s)	470, 572, 1097
MalI	GATC	3 cut(s)	183, 356, 839
MboI	GATC	3 cut(s)	181, 354, 837
MboII	GAAGA	3 cut(s)	597, 1030, 1055
MfeI	CAATTG	3 cut(s)	255, 312, 927
MhlI	GDGCHC	1 cut(s)	989
MluCI	AATT	7 cut(s)	255, 312, 333, 390, 529, 692, 927
Mly113I	GGCGCC	1 cut(s)	13
MlyI	GAGTC	1 cut(s)	718
MmeI	TCCRAC	1 cut(s)	760
MroXI	GAANNNNTTC	1 cut(s)	130
MseI	TTAA	5 cut(s)	332, 491, 504, 1014, 1153
MspA1I	CMGCKG	1 cut(s)	759
MspCI	CTTAAG	1 cut(s)	1013
MspI	CCGG	1 cut(s)	570
MspR9I	CCNGG	1 cut(s)	704
MunI	CAATTG	3 cut(s)	255, 312, 927
MvaI	CCWGG	1 cut(s)	704
MvnI	CGCG	4 cut(s)	321, 500, 759, 1082
MwoI	GCNNNNNNNGC	7 cut(s)	173, 325, 599, 747, 756, 914, 1114
NarI	GGCGCC	1 cut(s)	13
NdeI	CATATG	1 cut(s)	121
NdeII	GATC	3 cut(s)	181, 354, 837
NheI	GCTAGC	1 cut(s)	872
NlaIII	CATG	3 cut(s)	53, 160, 171
NlaIV	GGNNCC	2 cut(s)	14, 601
NspI	RCATGY	1 cut(s)	171
PagI	TCATGA	1 cut(s)	49
PceI	AGGCCT	2 cut(s)	94, 488
PdmI	GAANNNNTTC	1 cut(s)	130
PfeI	GAWTC	3 cut(s)	659, 783, 821
PflFI	GACNNNGTC	1 cut(s)	935
PkrI	GCNGC	7 cut(s)	142, 286, 304, 307, 595, 670, 761
PleI	GAGTC	1 cut(s)	718
PluTI	GGCGCC	1 cut(s)	16
PpsI	GAGTC	1 cut(s)	718
Psp124BI	GAGCTC	1 cut(s)	989
Psp6I	CCWGG	1 cut(s)	702
PspGI	CCWGG	1 cut(s)	702
PspN4I	GGNNCC	2 cut(s)	14, 601
PstNI	CAGNNNCTG	1 cut(s)	1076
PsyI	GACNNNGTC	1 cut(s)	935
RsaI	GTAC	2 cut(s)	643, 980
RsaNI	GTAC	2 cut(s)	642, 979
SacI	GAGCTC	1 cut(s)	989
SacII	CCGCGG	1 cut(s)	760
SaqAI	TTAA	5 cut(s)	332, 491, 504, 1014, 1153
SatI	GCNGC	7 cut(s)	141, 285, 303, 306, 594, 669, 760
Sau3AI	GATC	3 cut(s)	181, 354, 837
SchI	GAGTC	1 cut(s)	718
ScrFI	CCNGG	1 cut(s)	704
SduI	GDGCHC	1 cut(s)	989
SfaNI	GCATC	2 cut(s)	590, 926
SfoI	GGCGCC	1 cut(s)	14
Sfr303I	CCGCGG	1 cut(s)	760
SgrBI	CCGCGG	1 cut(s)	760
SmlI	CTYRAG	1 cut(s)	1013
SmoI	CTYRAG	1 cut(s)	1013
Sse9I	AATT	7 cut(s)	255, 312, 333, 390, 529, 692, 927
SseBI	AGGCCT	2 cut(s)	94, 488
SsiI	CCGC	4 cut(s)	596, 757, 759, 1082
SspDI	GGCGCC	1 cut(s)	12
SspMI	CTAG	2 cut(s)	873, 1118
SstI	GAGCTC	1 cut(s)	989
StuI	AGGCCT	2 cut(s)	94, 488
StyD4I	CCNGG	1 cut(s)	702
StyI	CCWWGG	2 cut(s)	663, 883
TaaI	ACNGT	2 cut(s)	797, 937
TaiI	ACGT	1 cut(s)	202
TaqI	TCGA	3 cut(s)	172, 219, 993
TasI	AATT	7 cut(s)	255, 312, 333, 390, 529, 692, 927
TatI	WGTACW	1 cut(s)	978
TauI	GCSGC	1 cut(s)	762
TfiI	GAWTC	3 cut(s)	659, 783, 821
Tru1I	TTAA	5 cut(s)	332, 491, 504, 1014, 1153
Tru9I	TTAA	5 cut(s)	332, 491, 504, 1014, 1153
TscAI	CASTG	2 cut(s)	850, 940
TseI	GCWGC	6 cut(s)	140, 284, 302, 305, 593, 668
TspDTI	ATGAA	3 cut(s)	108, 173, 431
TspGWI	ACGGA	4 cut(s)	47, 174, 458, 929
TspRI	CASTG	2 cut(s)	850, 940
Tth111I	GACNNNGTC	1 cut(s)	935
Vha464I	CTTAAG	1 cut(s)	1013
XceI	RCATGY	1 cut(s)	171
XmiI	GTMKAC	2 cut(s)	621, 714
XmnI	GAANNNNTTC	1 cut(s)	130
XspI	CTAG	2 cut(s)	873, 1118

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.