Rroxscaffold_4G00331400

Belongs to the mitochondrial carrier (TC 2.A.29) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001633
Physical Location & Seq
Reverse (-)
65310260 .. 65313843
3584 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00331400.1

Sequence Viewer

Length: 1158 bp
ATGGTTGATCAGGTTCAACACCCTACTGTCATGCAAAAGGTAGCTGGCCAGCTCCTTCAGCAATCCATTCAGGGATATGATACTGGCTTCCAAAGACAGGGGATGTACCAGAGACGTCCTGCATATGGAAATTATTCCAATGCTGCATTGCAATATCCCATGATGCCAGCTTGCAGAGCTACCACTGATCTGTCCATGATTCCATCAACTGCATCCCCAGTTTTTGTGCAAGCCCCCGCAGAGAAAAGCCACTTTGTCATTGATTTCCTTATGGGTGGTGTTTCTGCTGCTGTATCTAAGACCGCTGCTGCTCCCATTGAGCGTGTCAAGCTTTTGATTCAGAACCAGGATGAAATGATCAAGTCTGGTCGGTTGTCTCAACCCTACAAGGGCATCGGTGAGTGCTTCTCCAGAACAATCAAGGAAGAAGGATTTGGTTCATTGTGGAGAGGAAACACTGCCAACGTCATCCGTTACTTCCCCACTCAGGCATTGAACTTTGCATTCAAGGACTACTTCAAGAGGCTATTCAATTTCAAGAAAGACAGGGATGGTTATTGGAAGTGGTTTGCTGGTAACTTGGCCTCAGGAGGTGCGGCTGGTGCTTCTTCTCTTTTCTTTGTCTACTCTTTGGACTATGCTCGTACCCGTTTGGCTAATGATTCTAAGGCTGCAAAGAAGGGAGGAGAGAGACAATTCAATGGTCTGATTGATGTCTACAAGAAGACATTGAAATCTGATGGTATTGCTGGTCTATACCGTGGATTCAATATATCATGTGTTGGTATCATTGTTTACCGTGGTTTGTACTTTGGACTGTATGATTCACTCAAGCCTGTGGTCCTAACGGGAGGTTTGCAGGATAGTTTCTTTGCTAGCTTTGCTCTTGGATGGGTCATCACTAATGGTGCTGGTCTTGCTTCCTACCCAATTGACACTGTTCGTAGAAGAATGATGATGACCTCCGGTGAAGCCGTCAAGTACAAGAGTTCTTTTGATGCCTTCACTCAGATCTTGAAGAATGAGGGTGCCAAGTCCCTGTTCAAGGGTGCTGGTGCAAACATCCTCCGTGCCATTGCTGGTGCTGGTGTCCTTTCTGGTTATGACAAGTTGCAGCTGATTGTGTTCGGGAAGAAGTATGGTTCTGGATCTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000295 GO:0003674 GO:0005215 GO:0005346 GO:0005347 GO:0005471 GO:0005488 GO:0005507 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0005773 GO:0005774 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006839 GO:0006855 GO:0006862 GO:0006996 GO:0007005 GO:0007006 GO:0008150 GO:0008509 GO:0008514 GO:0009507 GO:0009526 GO:0009536 GO:0009941 GO:0009987 GO:0015075 GO:0015215 GO:0015216 GO:0015217 GO:0015238 GO:0015291 GO:0015297 GO:0015301 GO:0015605 GO:0015711 GO:0015748 GO:0015865 GO:0015866 GO:0015867 GO:0015868 GO:0015893 GO:0015931 GO:0015932 GO:0016020 GO:0016021 GO:0016043 GO:0019866 GO:0022804 GO:0022857 GO:0030312 GO:0031090 GO:0031224 GO:0031966 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0034220 GO:0042221 GO:0042493 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044429 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046872 GO:0046902 GO:0046914 GO:0050896 GO:0051179 GO:0051234 GO:0051503 GO:0055085 GO:0061024 GO:0065007 GO:0065008 GO:0070013 GO:0071702 GO:0071705 GO:0071840 GO:0071944 GO:0090559 GO:0098588 GO:0098656 GO:0098805 GO:0099516 GO:1901264 GO:1901505 GO:1901679
KEGG Pathways
Metabolic & Signaling
ko00514 ko04020 ko04022 ko04217 ko04218 ko05012 ko05016 ko05166 map00514 map04020 map04022 map04217 map04218 map05012 map05016 map05166
Pfam Domains
Protein Families
Mito_carr

Protein Analysis

385

Amino Acids

42.17

Weight (kDa)

9.77

Isoelectric Point (pI)

29.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mito_carr PF00153 82 - 180 1.5e-26 Mitochondrial carrier protein
Mito_carr PF00153 188 - 281 5.3e-21 Mitochondrial carrier protein
Mito_carr PF00153 288 - 376 2e-17 Mitochondrial carrier protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 118
AccB1I GGYRCC 1 cut(s) 1028
AccI GTMKAC 2 cut(s) 624, 717
AciI CCGC 3 cut(s) 237, 303, 596
AclWI GGATC 1 cut(s) 1156
AcoI YGGCCR 1 cut(s) 46
AcuI CTGAAG 1 cut(s) 41
AcyI GRCGYC 1 cut(s) 115
AfaI GTAC 4 cut(s) 107, 646, 809, 983
AfiI CCNNNNNNNGG 5 cut(s) 97, 125, 389, 487, 1045
AjnI CCWGG 1 cut(s) 345
AjuI GAANNNNNNNTTGG 4 cut(s) 417, 449, 1025, 1057
AluBI AGCT 7 cut(s) 44, 52, 170, 179, 331, 879, 1117
AluI AGCT 7 cut(s) 44, 52, 170, 179, 331, 879, 1117
Alw26I GTCTC 3 cut(s) 106, 381, 685
AlwI GGATC 1 cut(s) 1156
AoxI GGCC 2 cut(s) 46, 582
ApeKI GCWGC 6 cut(s) 143, 287, 305, 308, 671, 1114
Asp700I GAANNNNTTC 1 cut(s) 133
AspS9I GGNCC 1 cut(s) 841
AsuHPI GGTGA 2 cut(s) 410, 980
AsuNHI GCTAGC 1 cut(s) 875
AvaII GGWCC 1 cut(s) 841
AxyI CCTNAGG 1 cut(s) 586
BalI TGGCCA 1 cut(s) 48
BanI GGYRCC 1 cut(s) 1028
BbsI GAAGAC 1 cut(s) 731
BbvI GCAGC 6 cut(s) 130, 274, 292, 295, 658, 1126
BccI CCATC 4 cut(s) 211, 545, 734, 885
BceAI ACGGC 1 cut(s) 959
BciT130I CCWGG 1 cut(s) 347
BclI TGATCA 2 cut(s) 7, 357
BcoDI GTCTC 3 cut(s) 106, 381, 685
BfaI CTAG 1 cut(s) 876
BglII AGATCT 1 cut(s) 1011
BisI GCNGC 7 cut(s) 144, 288, 306, 309, 597, 672, 1115
BlsI GCNGC 7 cut(s) 145, 289, 307, 310, 598, 673, 1116
Bme1390I CCNGG 1 cut(s) 347
Bme18I GGWCC 1 cut(s) 841
BmgT120I GGNCC 1 cut(s) 841
BmiI GGNNCC 1 cut(s) 1030
BmrFI CCNGG 1 cut(s) 347
BmrI ACTGGG 1 cut(s) 212
BmsI GCATC 4 cut(s) 153, 221, 402, 988
BmtI GCTAGC 1 cut(s) 879
BmuI ACTGGG 1 cut(s) 212
BpiI GAAGAC 1 cut(s) 731
BplI GAGNNNNNCTC 2 cut(s) 392, 424
BpmI CTGGAG 1 cut(s) 394
BpuEI CTTGAG 1 cut(s) 815
BsaHI GRCGYC 1 cut(s) 115
BsaJI CCNNGG 2 cut(s) 760, 799
BsaWI WCCGGW 1 cut(s) 965
Bsc4I CCNNNNNNNGG 5 cut(s) 97, 125, 389, 487, 1045
Bse1I ACTGG 2 cut(s) 88, 218
Bse21I CCTNAGG 1 cut(s) 586
Bse3DI GCAATG 2 cut(s) 146, 1074
BseBI CCWGG 1 cut(s) 347
BseDI CCNNGG 2 cut(s) 760, 799
BseGI GGATG 7 cut(s) 108, 212, 355, 468, 556, 896, 1062
BseLI CCNNNNNNNGG 5 cut(s) 97, 125, 389, 487, 1045
BseMI GCAATG 2 cut(s) 146, 1074
BseMII CTCAG 3 cut(s) 500, 600, 1022
BseNI ACTGG 2 cut(s) 88, 218
BseRI GAGGAG 1 cut(s) 699
BseXI GCAGC 6 cut(s) 130, 274, 292, 295, 658, 1126
BshFI GGCC 2 cut(s) 48, 584
BshNI GGYRCC 1 cut(s) 1028
BsiSI CCGG 1 cut(s) 966
BslFI GGGAC 1 cut(s) 1021
BslI CCNNNNNNNGG 5 cut(s) 97, 125, 389, 487, 1045
BsmAI GTCTC 3 cut(s) 106, 381, 685
BsmBI CGTCTC 1 cut(s) 106
BsmFI GGGAC 1 cut(s) 1021
BsmI GAATGC 1 cut(s) 503
BsnI GGCC 2 cut(s) 48, 584
Bsp143I GATC 5 cut(s) 7, 187, 357, 1011, 1148
BspACI CCGC 3 cut(s) 237, 303, 596
BspANI GGCC 2 cut(s) 48, 584
BspCNI CTCAG 3 cut(s) 499, 599, 1021
BspLI GGNNCC 1 cut(s) 1030
BspOI GCTAGC 1 cut(s) 879
BspPI GGATC 1 cut(s) 1156
BspT107I GGYRCC 1 cut(s) 1028
BsrDI GCAATG 2 cut(s) 146, 1074
BsrI ACTGG 2 cut(s) 88, 218
BssECI CCNNGG 2 cut(s) 760, 799
BssMI GATC 5 cut(s) 7, 187, 357, 1011, 1148
BssNI GRCGYC 1 cut(s) 115
Bst2UI CCWGG 1 cut(s) 347
Bst4CI ACNGT 5 cut(s) 28, 761, 800, 819, 940
BstACI GRCGYC 1 cut(s) 115
BstC8I GCNNGC 6 cut(s) 46, 50, 168, 172, 231, 877
BstDEI CTNAG 5 cut(s) 297, 486, 586, 666, 1008
BstDSI CCRYGG 2 cut(s) 760, 799
BstENI CCTNNNNNAGG 1 cut(s) 1043
BstF5I GGATG 7 cut(s) 108, 212, 355, 468, 556, 896, 1062
BstKTI GATC 5 cut(s) 10, 190, 360, 1014, 1151
BstMAI GTCTC 3 cut(s) 106, 381, 685
BstMBI GATC 5 cut(s) 7, 187, 357, 1011, 1148
BstMWI GCNNNNNNNGC 6 cut(s) 58, 176, 328, 602, 881, 917
BstNI CCWGG 1 cut(s) 347
BstSCI CCNGG 1 cut(s) 345
BstV1I GCAGC 6 cut(s) 130, 274, 292, 295, 658, 1126
BstV2I GAAGAC 1 cut(s) 731
BstX2I RGATCY 2 cut(s) 1011, 1148
BstYI RGATCY 2 cut(s) 1011, 1148
Bsu36I CCTNAGG 1 cut(s) 586
BsuRI GGCC 2 cut(s) 48, 584
BtgI CCRYGG 2 cut(s) 760, 799
BtsCI GGATG 7 cut(s) 108, 212, 355, 468, 556, 896, 1062
BtsI GCAGTG 1 cut(s) 456
BtsIMutI CAGTG 3 cut(s) 183, 456, 936
Cac8I GCNNGC 6 cut(s) 46, 50, 168, 172, 231, 877
Cfr13I GGNCC 1 cut(s) 841
Csp6I GTAC 4 cut(s) 106, 645, 808, 982
CviAII CATG 4 cut(s) 31, 160, 196, 777
CviQI GTAC 4 cut(s) 106, 645, 808, 982
DdeI CTNAG 5 cut(s) 297, 486, 586, 666, 1008
DpnI GATC 5 cut(s) 9, 189, 359, 1013, 1150
DpnII GATC 5 cut(s) 7, 187, 357, 1011, 1148
EaeI YGGCCR 1 cut(s) 46
Eco47I GGWCC 1 cut(s) 841
Eco57I CTGAAG 1 cut(s) 41
Eco81I CCTNAGG 1 cut(s) 586
EcoNI CCTNNNNNAGG 1 cut(s) 1043
EcoRII CCWGG 1 cut(s) 345
Esp3I CGTCTC 1 cut(s) 106
FaeI CATG 4 cut(s) 34, 163, 199, 780
FalI AAGNNNNNCTT 2 cut(s) 500, 532
FaqI GGGAC 1 cut(s) 1021
FatI CATG 4 cut(s) 30, 159, 195, 776
FauI CCCGC 1 cut(s) 244
FauNDI CATATG 1 cut(s) 124
FbaI TGATCA 2 cut(s) 7, 357
FblI GTMKAC 2 cut(s) 624, 717
Fnu4HI GCNGC 7 cut(s) 144, 288, 306, 309, 597, 672, 1115
FokI GGATG 7 cut(s) 115, 199, 362, 455, 563, 903, 1049
Fsp4HI GCNGC 7 cut(s) 144, 288, 306, 309, 597, 672, 1115
FspBI CTAG 1 cut(s) 876
GluI GCNGC 7 cut(s) 144, 288, 306, 309, 597, 672, 1115
GsuI CTGGAG 1 cut(s) 394
HaeIII GGCC 2 cut(s) 48, 584
HapII CCGG 1 cut(s) 966
Hin1I GRCGYC 1 cut(s) 115
Hin1II CATG 4 cut(s) 34, 163, 199, 780
HindIII AAGCTT 1 cut(s) 329
HinfI GANTC 5 cut(s) 199, 337, 662, 765, 824
HpaII CCGG 1 cut(s) 966
HphI GGTGA 2 cut(s) 410, 980
Hpy166II GTNNAC 3 cut(s) 625, 718, 796
Hpy188I TCNGA 4 cut(s) 342, 708, 739, 1011
Hpy188III TCNNGA 7 cut(s) 411, 520, 538, 588, 1015, 1129, 1146
Hpy8I GTNNAC 3 cut(s) 625, 718, 796
HpyAV CCTTC 4 cut(s) 65, 422, 673, 1012
HpyCH4III ACNGT 5 cut(s) 28, 761, 800, 819, 940
HpyCH4IV ACGT 2 cut(s) 115, 465
HpyF10VI GCNNNNNNNGC 6 cut(s) 58, 176, 328, 602, 881, 917
HpyF3I CTNAG 5 cut(s) 297, 486, 586, 666, 1008
HpySE526I ACGT 2 cut(s) 115, 465
Hsp92I GRCGYC 1 cut(s) 115
Hsp92II CATG 4 cut(s) 34, 163, 199, 780
Ksp22I TGATCA 2 cut(s) 7, 357
Kzo9I GATC 5 cut(s) 7, 187, 357, 1011, 1148
LmnI GCTCC 2 cut(s) 57, 316
Lsp1109I GCAGC 6 cut(s) 130, 274, 292, 295, 658, 1126
LweI GCATC 4 cut(s) 153, 221, 402, 988
MaeI CTAG 1 cut(s) 876
MaeII ACGT 2 cut(s) 115, 465
MaeIII GTNAC 2 cut(s) 473, 575
MalI GATC 5 cut(s) 9, 189, 359, 1013, 1150
MboI GATC 5 cut(s) 7, 187, 357, 1011, 1148
MboII GAAGA 6 cut(s) 437, 600, 736, 960, 1030, 1144
MfeI CAATTG 1 cut(s) 930
MflI RGATCY 2 cut(s) 1011, 1148
MlsI TGGCCA 1 cut(s) 48
MluCI AATT 4 cut(s) 130, 532, 695, 930
MluNI TGGCCA 1 cut(s) 48
MnlI CCTC 9 cut(s) 443, 516, 584, 595, 677, 845, 973, 1018, 1076
Mox20I TGGCCA 1 cut(s) 48
MroXI GAANNNNTTC 1 cut(s) 133
MscI TGGCCA 1 cut(s) 48
MseI TTAA 1 cut(s) 1156
Msp20I TGGCCA 1 cut(s) 48
MspA1I CMGCKG 2 cut(s) 305, 1117
MspI CCGG 1 cut(s) 966
MspR9I CCNGG 1 cut(s) 347
MunI CAATTG 1 cut(s) 930
Mva1269I GAATGC 1 cut(s) 503
MvaI CCWGG 1 cut(s) 347
MwoI GCNNNNNNNGC 6 cut(s) 58, 176, 328, 602, 881, 917
NdeI CATATG 1 cut(s) 124
NdeII GATC 5 cut(s) 7, 187, 357, 1011, 1148
NheI GCTAGC 1 cut(s) 875
NlaIII CATG 4 cut(s) 34, 163, 199, 780
NlaIV GGNNCC 1 cut(s) 1030
PctI GAATGC 1 cut(s) 503
PdmI GAANNNNTTC 1 cut(s) 133
PfeI GAWTC 5 cut(s) 199, 337, 662, 765, 824
PkrI GCNGC 7 cut(s) 145, 289, 307, 310, 598, 673, 1116
Psp6I CCWGG 1 cut(s) 345
PspGI CCWGG 1 cut(s) 345
PspN4I GGNNCC 1 cut(s) 1030
PspPI GGNCC 1 cut(s) 841
PsuI RGATCY 2 cut(s) 1011, 1148
PvuII CAGCTG 1 cut(s) 1117
RsaI GTAC 4 cut(s) 107, 646, 809, 983
RsaNI GTAC 4 cut(s) 106, 645, 808, 982
SaqAI TTAA 1 cut(s) 1156
SatI GCNGC 7 cut(s) 144, 288, 306, 309, 597, 672, 1115
Sau3AI GATC 5 cut(s) 7, 187, 357, 1011, 1148
Sau96I GGNCC 1 cut(s) 841
ScrFI CCNGG 1 cut(s) 347
SfaNI GCATC 4 cut(s) 153, 221, 402, 988
SinI GGWCC 1 cut(s) 841
SmlI CTYRAG 1 cut(s) 830
SmoI CTYRAG 1 cut(s) 830
Sse9I AATT 4 cut(s) 130, 532, 695, 930
SsiI CCGC 3 cut(s) 237, 303, 596
SspMI CTAG 1 cut(s) 876
StyD4I CCNGG 1 cut(s) 345
TaaI ACNGT 5 cut(s) 28, 761, 800, 819, 940
TaiI ACGT 2 cut(s) 118, 468
TasI AATT 4 cut(s) 130, 532, 695, 930
TatI WGTACW 2 cut(s) 807, 981
TauI GCSGC 1 cut(s) 599
TfiI GAWTC 5 cut(s) 199, 337, 662, 765, 824
Tru1I TTAA 1 cut(s) 1156
Tru9I TTAA 1 cut(s) 1156
TscAI CASTG 3 cut(s) 190, 463, 943
TseI GCWGC 6 cut(s) 143, 287, 305, 308, 671, 1114
TspDTI ATGAA 2 cut(s) 366, 429
TspGWI ACGGA 2 cut(s) 461, 1058
TspRI CASTG 3 cut(s) 190, 463, 943
VpaK11BI GGWCC 1 cut(s) 841
XagI CCTNNNNNAGG 1 cut(s) 1043
XmiI GTMKAC 2 cut(s) 624, 717
XmnI GAANNNNTTC 1 cut(s) 133
XspI CTAG 1 cut(s) 876
ZraI GACGTC 1 cut(s) 116
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.