MD07G1008900.v1.1

Belongs to the mitochondrial carrier (TC 2.A.29) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0001633
Physical Location & Seq
Forward (+)
838325 .. 841503
3179 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1008900.v1.1.491

Sequence Viewer

Length: 1299 bp
ATGTGTCAGCCGTCAGCCAATAAAAAGGCCCCATTTCTCTCCAAAGAGGCGTCTCCTCCACACCTCTCTCGCTCTCGCTCTCCGTCTCTCTTTTGTCTTTGCGCATTTCAGCGTAGAGTTCAAAGCGGAGACGCCTCGCAGCCACCACCACCGAAGGTTCAACATCCTTCTGTCGTCCAGAAGGTAGCTGGCCAGCTCCTTCAGCAATCTATCCAGGGTTACGATAATGGCTTCCAAAGACCAGGGATGTACCAAAGACGTGCATATGGGAACTACACTAATGCTGCATTGCAGTATCCCTTCATGCCAGCATGCAGGGCTACCACTGATATGTCTTTGGTTCCATCAACTGCATCTCCTATCTTTGTTCAAGCCCCTGCAGAGAAAGGCCACTTTCTTATTGATTTTCTCATGGGCGGAGTCTCTGCTGCTGTATCTAAAACTGCTGCTGCCCCAATTGAGCGTGTCAAGCTGTTGATTCAGAACCAGGACGAGATGATCAAAAGTGGTCGTTTGTCTCAACCCTACAAGGGCATTGGAGAGTGCTTTAGCCGTACAATCAAGGAAGAGGGTTTTGGTTCATTGTGGAGAGGGAACACTGCCAATGTTATCCGTTACTTCCCCACTCAGGCCTTGAACTTTGCATTCAAGGATTACTTCAAGAGGCTGTTCAACTTCAAGAAAGACAGGGATGGTTATTGGAAATGGTTTGCTGGTAACTTGGCTTCAGGAAGTGCTGCTGGTGCTTATTCTCTTCTCTTTGTCTACTCTTTGGATTATGCTCGTACTCGTTTGACTAATGATGCTAAAGCTGCAAAGAAGGGAGGAGAAAGACAATTCAATGGTCTGGTTGATGTCTACAGGAAGACATTGAAATCTGATGGAATTGCCGGTCTTTACCGTGGATTCAACATCTCTTGTGTTGGAATTATTGTCTACCGTGGTCTTTACTTTGGACTGTACGATTCCCTAAAGCCTGTTGTCCTGACTGGAGGCTTGCAGGATAGTTTCTTCGCTAGCTTTGCCCTCGGTTGGGTCATCACCAATGGTGCTGGTCTTGCATCCTACCCAATTGACACTGTCCGCAGAAGAATGATGATGACCTCCGGTGAAGCCGTCAAGTACAAGAGTTCCCTGGATGCCTTCCAACAAATCTTGAAGAACGAGGGTGCCAAGTCTCTCTTCAAGGGTGCTGGTGCTAACATCCTCCGTGCCATTGCTGGTGCTGGTGTGCTGTCTGGTTATGACAAGCTACAGCTGCTTGTGTTCGGCAAGAAGTACGGATCTGGTTCCGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000295 GO:0003674 GO:0005215 GO:0005346 GO:0005347 GO:0005471 GO:0005488 GO:0005507 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0005773 GO:0005774 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006839 GO:0006855 GO:0006862 GO:0006996 GO:0007005 GO:0007006 GO:0008150 GO:0008509 GO:0008514 GO:0009507 GO:0009526 GO:0009536 GO:0009941 GO:0009987 GO:0015075 GO:0015215 GO:0015216 GO:0015217 GO:0015238 GO:0015291 GO:0015297 GO:0015301 GO:0015605 GO:0015711 GO:0015748 GO:0015865 GO:0015866 GO:0015867 GO:0015868 GO:0015893 GO:0015931 GO:0015932 GO:0016020 GO:0016021 GO:0016043 GO:0019866 GO:0022804 GO:0022857 GO:0030312 GO:0031090 GO:0031224 GO:0031966 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0034220 GO:0042221 GO:0042493 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044429 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046872 GO:0046902 GO:0046914 GO:0050896 GO:0051179 GO:0051234 GO:0051503 GO:0055085 GO:0061024 GO:0065007 GO:0065008 GO:0070013 GO:0071702 GO:0071705 GO:0071840 GO:0071944 GO:0090559 GO:0098588 GO:0098656 GO:0098805 GO:0099516 GO:1901264 GO:1901505 GO:1901679
KEGG Pathways
Metabolic & Signaling
ko00514 ko04020 ko04022 ko04217 ko04218 ko05012 ko05016 ko05166 map00514 map04020 map04022 map04217 map04218 map05012 map05016 map05166
Pfam Domains
Protein Families
Mito_carr

Protein Analysis

433

Amino Acids

47.29

Weight (kDa)

9.86

Isoelectric Point (pI)

39.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mito_carr PF00153 131 - 227 3.2e-26 Mitochondrial carrier protein
Mito_carr PF00153 235 - 328 3.9e-20 Mitochondrial carrier protein
Mito_carr PF00153 335 - 424 2.4e-17 Mitochondrial carrier protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 103
AccB1I GGYRCC 1 cut(s) 1169
AccI GTMKAC 3 cut(s) 765, 858, 936
AciI CCGC 3 cut(s) 126, 417, 1084
AclWI GGATC 1 cut(s) 1291
AcoI YGGCCR 1 cut(s) 190
AcuI CTGAAG 2 cut(s) 185, 711
AcyI GRCGYC 2 cut(s) 50, 132
AfaI GTAC 6 cut(s) 251, 556, 787, 962, 1124, 1280
AfiI CCNNNNNNNGG 4 cut(s) 530, 628, 1032, 1033
AjiI CACGTC 1 cut(s) 260
AjnI CCWGG 4 cut(s) 213, 241, 486, 1134
AjuI GAANNNNNNNTTGG 4 cut(s) 558, 590, 1166, 1198
AluBI AGCT 7 cut(s) 188, 196, 472, 812, 1020, 1252, 1258
AluI AGCT 7 cut(s) 188, 196, 472, 812, 1020, 1252, 1258
Alw26I GTCTC 6 cut(s) 57, 90, 123, 427, 522, 1182
AlwI GGATC 1 cut(s) 1291
AoxI GGCC 4 cut(s) 27, 190, 388, 630
ApeKI GCWGC 8 cut(s) 139, 284, 428, 446, 449, 737, 812, 1258
AspLEI GCGC 1 cut(s) 104
AspS9I GGNCC 1 cut(s) 28
AsuHPI GGTGA 2 cut(s) 1033, 1121
AsuNHI GCTAGC 1 cut(s) 1016
BalI TGGCCA 1 cut(s) 192
BanI GGYRCC 1 cut(s) 1169
BarI GAAGNNNNNNTAC 2 cut(s) 709, 741
BbsI GAAGAC 1 cut(s) 872
BbvI GCAGC 8 cut(s) 151, 271, 415, 433, 436, 724, 799, 1245
BccI CCATC 3 cut(s) 352, 686, 875
BceAI ACGGC 2 cut(s) 537, 1100
BciT130I CCWGG 4 cut(s) 215, 243, 488, 1136
BciVI GTATCC 1 cut(s) 306
BclI TGATCA 1 cut(s) 498
BcoDI GTCTC 6 cut(s) 57, 90, 123, 427, 522, 1182
BfaI CTAG 1 cut(s) 1017
BfmI CTRYAG 3 cut(s) 378, 859, 1253
BfuI GTATCC 1 cut(s) 306
BisI GCNGC 8 cut(s) 140, 285, 429, 447, 450, 738, 813, 1259
BlsI GCNGC 8 cut(s) 141, 286, 430, 448, 451, 739, 814, 1260
Bme1390I CCNGG 4 cut(s) 215, 243, 488, 1136
BmgBI CACGTC 1 cut(s) 260
BmgT120I GGNCC 1 cut(s) 28
BmiI GGNNCC 4 cut(s) 30, 342, 1171, 1291
BmrFI CCNGG 4 cut(s) 215, 243, 488, 1136
BmsI GCATC 4 cut(s) 362, 793, 1070, 1129
BmtI GCTAGC 1 cut(s) 1020
BpiI GAAGAC 1 cut(s) 872
BpmI CTGGAG 1 cut(s) 1011
BsaHI GRCGYC 2 cut(s) 50, 132
BsaJI CCNNGG 6 cut(s) 214, 242, 901, 940, 1027, 1134
BsaWI WCCGGW 2 cut(s) 1106, 1292
BsaXI ACNNNNNCTCC 2 cut(s) 340, 370
Bsc4I CCNNNNNNNGG 4 cut(s) 530, 628, 1032, 1033
Bse118I RCCGGY 1 cut(s) 890
Bse1I ACTGG 1 cut(s) 994
Bse3DI GCAATG 2 cut(s) 287, 1215
BseBI CCWGG 4 cut(s) 215, 243, 488, 1136
BseDI CCNNGG 6 cut(s) 214, 242, 901, 940, 1027, 1134
BseGI GGATG 6 cut(s) 163, 252, 697, 1061, 1144, 1203
BseLI CCNNNNNNNGG 4 cut(s) 530, 628, 1032, 1033
BseMI GCAATG 2 cut(s) 287, 1215
BseMII CTCAG 1 cut(s) 641
BseNI ACTGG 1 cut(s) 994
BseRI GAGGAG 2 cut(s) 45, 840
BseXI GCAGC 8 cut(s) 151, 271, 415, 433, 436, 724, 799, 1245
BshFI GGCC 4 cut(s) 29, 192, 390, 632
BshNI GGYRCC 1 cut(s) 1169
BsiSI CCGG 3 cut(s) 891, 1107, 1293
BslI CCNNNNNNNGG 4 cut(s) 530, 628, 1032, 1033
BsmAI GTCTC 6 cut(s) 57, 90, 123, 427, 522, 1182
BsmBI CGTCTC 3 cut(s) 57, 90, 123
BsmI GAATGC 1 cut(s) 644
BsnI GGCC 4 cut(s) 29, 192, 390, 632
Bsp143I GATC 2 cut(s) 498, 1283
BspACI CCGC 3 cut(s) 126, 417, 1084
BspANI GGCC 4 cut(s) 29, 192, 390, 632
BspCNI CTCAG 1 cut(s) 640
BspLI GGNNCC 4 cut(s) 30, 342, 1171, 1291
BspMAI CTGCAG 1 cut(s) 382
BspOI GCTAGC 1 cut(s) 1020
BspPI GGATC 1 cut(s) 1291
BspT107I GGYRCC 1 cut(s) 1169
BsrDI GCAATG 2 cut(s) 287, 1215
BsrFI RCCGGY 1 cut(s) 890
BsrI ACTGG 1 cut(s) 994
BssAI RCCGGY 1 cut(s) 890
BssECI CCNNGG 6 cut(s) 214, 242, 901, 940, 1027, 1134
BssMI GATC 2 cut(s) 498, 1283
BssNI GRCGYC 2 cut(s) 50, 132
Bst2UI CCWGG 4 cut(s) 215, 243, 488, 1136
Bst4CI ACNGT 4 cut(s) 902, 941, 960, 1081
Bst6I CTCTTC 3 cut(s) 561, 759, 1187
BstACI GRCGYC 2 cut(s) 50, 132
BstC8I GCNNGC 6 cut(s) 190, 194, 309, 313, 998, 1018
BstDEI CTNAG 1 cut(s) 627
BstDSI CCRYGG 2 cut(s) 901, 940
BstF5I GGATG 6 cut(s) 163, 252, 697, 1061, 1144, 1203
BstHHI GCGC 1 cut(s) 104
BstKTI GATC 2 cut(s) 501, 1286
BstMAI GTCTC 6 cut(s) 57, 90, 123, 427, 522, 1182
BstMBI GATC 2 cut(s) 498, 1283
BstMWI GCNNNNNNNGC 8 cut(s) 202, 317, 469, 743, 812, 1022, 1058, 1258
BstNI CCWGG 4 cut(s) 215, 243, 488, 1136
BstNSI RCATGY 1 cut(s) 315
BstSCI CCNGG 4 cut(s) 213, 241, 486, 1134
BstSFI CTRYAG 3 cut(s) 378, 859, 1253
BstV1I GCAGC 8 cut(s) 151, 271, 415, 433, 436, 724, 799, 1245
BstV2I GAAGAC 1 cut(s) 872
BstX2I RGATCY 1 cut(s) 1283
BstYI RGATCY 1 cut(s) 1283
BsuI GTATCC 1 cut(s) 306
BsuRI GGCC 4 cut(s) 29, 192, 390, 632
BtgI CCRYGG 2 cut(s) 901, 940
BtrI CACGTC 1 cut(s) 260
BtsCI GGATG 6 cut(s) 163, 252, 697, 1061, 1144, 1203
BtsI GCAGTG 1 cut(s) 597
BtsIMutI CAGTG 3 cut(s) 324, 597, 1077
Cac8I GCNNGC 6 cut(s) 190, 194, 309, 313, 998, 1018
CfoI GCGC 1 cut(s) 104
Cfr10I RCCGGY 1 cut(s) 890
Cfr13I GGNCC 1 cut(s) 28
CseI GACGC 2 cut(s) 39, 140
Csp6I GTAC 6 cut(s) 250, 555, 786, 961, 1123, 1279
CviAII CATG 3 cut(s) 304, 312, 412
CviQI GTAC 6 cut(s) 250, 555, 786, 961, 1123, 1279
DdeI CTNAG 1 cut(s) 627
DpnI GATC 2 cut(s) 500, 1285
DpnII GATC 2 cut(s) 498, 1283
EaeI YGGCCR 1 cut(s) 190
Eam1104I CTCTTC 3 cut(s) 561, 759, 1187
EarI CTCTTC 3 cut(s) 561, 759, 1187
EciI GGCGGA 1 cut(s) 432
Eco147I AGGCCT 1 cut(s) 632
Eco57I CTGAAG 2 cut(s) 185, 711
EcoO109I RGGNCCY 1 cut(s) 28
EcoRII CCWGG 4 cut(s) 213, 241, 486, 1134
Esp3I CGTCTC 3 cut(s) 57, 90, 123
FaeI CATG 3 cut(s) 307, 315, 415
FaiI YATR 8 cut(s) 265, 267, 305, 313, 332, 413, 780, 1245
FalI AAGNNNNNCTT 4 cut(s) 641, 673, 1166, 1198
FatI CATG 3 cut(s) 303, 311, 411
FauNDI CATATG 1 cut(s) 265
FbaI TGATCA 1 cut(s) 498
FblI GTMKAC 3 cut(s) 765, 858, 936
Fnu4HI GCNGC 8 cut(s) 140, 285, 429, 447, 450, 738, 813, 1259
FokI GGATG 6 cut(s) 150, 259, 704, 1048, 1151, 1190
Fsp4HI GCNGC 8 cut(s) 140, 285, 429, 447, 450, 738, 813, 1259
FspBI CTAG 1 cut(s) 1017
FspI TGCGCA 1 cut(s) 103
GlaI GCGC 1 cut(s) 103
GluI GCNGC 8 cut(s) 140, 285, 429, 447, 450, 738, 813, 1259
GsuI CTGGAG 1 cut(s) 1011
HaeIII GGCC 4 cut(s) 29, 192, 390, 632
HapII CCGG 3 cut(s) 891, 1107, 1293
HgaI GACGC 2 cut(s) 39, 140
HhaI GCGC 1 cut(s) 104
Hin1I GRCGYC 2 cut(s) 50, 132
Hin1II CATG 3 cut(s) 307, 315, 415
Hin6I GCGC 1 cut(s) 102
HinP1I GCGC 1 cut(s) 102
HinfI GANTC 4 cut(s) 420, 478, 906, 965
HpaII CCGG 3 cut(s) 891, 1107, 1293
HphI GGTGA 2 cut(s) 1033, 1121
Hpy166II GTNNAC 3 cut(s) 766, 859, 937
Hpy188I TCNGA 2 cut(s) 483, 880
Hpy188III TCNNGA 6 cut(s) 178, 661, 679, 729, 985, 1156
Hpy8I GTNNAC 3 cut(s) 766, 859, 937
HpyAV CCTTC 7 cut(s) 148, 175, 177, 209, 310, 814, 1153
HpyCH4III ACNGT 4 cut(s) 902, 941, 960, 1081
HpyCH4IV ACGT 1 cut(s) 259
HpyF10VI GCNNNNNNNGC 8 cut(s) 202, 317, 469, 743, 812, 1022, 1058, 1258
HpyF3I CTNAG 1 cut(s) 627
HpySE526I ACGT 1 cut(s) 259
Hsp92I GRCGYC 2 cut(s) 50, 132
Hsp92II CATG 3 cut(s) 307, 315, 415
HspAI GCGC 1 cut(s) 102
Ksp22I TGATCA 1 cut(s) 498
Kzo9I GATC 2 cut(s) 498, 1283
LmnI GCTCC 1 cut(s) 201
Lsp1109I GCAGC 8 cut(s) 151, 271, 415, 433, 436, 724, 799, 1245
LweI GCATC 4 cut(s) 362, 793, 1070, 1129
MaeI CTAG 1 cut(s) 1017
MaeII ACGT 1 cut(s) 259
MaeIII GTNAC 3 cut(s) 218, 614, 716
MalI GATC 2 cut(s) 500, 1285
MboI GATC 2 cut(s) 498, 1283
MboII GAAGA 7 cut(s) 578, 746, 877, 1003, 1101, 1171, 1174
MfeI CAATTG 2 cut(s) 456, 1071
MflI RGATCY 1 cut(s) 1283
MlsI TGGCCA 1 cut(s) 192
MluCI AATT 5 cut(s) 456, 836, 885, 927, 1071
MluNI TGGCCA 1 cut(s) 192
MlyI GAGTC 1 cut(s) 429
MmeI TCCRAC 2 cut(s) 904, 1171
Mox20I TGGCCA 1 cut(s) 192
MscI TGGCCA 1 cut(s) 192
MseI TTAA 1 cut(s) 1297
MslI CAYNNNNRTG 1 cut(s) 329
Msp20I TGGCCA 1 cut(s) 192
MspA1I CMGCKG 1 cut(s) 1258
MspI CCGG 3 cut(s) 891, 1107, 1293
MspR9I CCNGG 4 cut(s) 215, 243, 488, 1136
MunI CAATTG 2 cut(s) 456, 1071
Mva1269I GAATGC 1 cut(s) 644
MvaI CCWGG 4 cut(s) 215, 243, 488, 1136
MwoI GCNNNNNNNGC 8 cut(s) 202, 317, 469, 743, 812, 1022, 1058, 1258
NdeI CATATG 1 cut(s) 265
NdeII GATC 2 cut(s) 498, 1283
NheI GCTAGC 1 cut(s) 1016
NlaIII CATG 3 cut(s) 307, 315, 415
NlaIV GGNNCC 4 cut(s) 30, 342, 1171, 1291
NsbI TGCGCA 1 cut(s) 103
NspI RCATGY 1 cut(s) 315
PaeI GCATGC 1 cut(s) 315
PceI AGGCCT 1 cut(s) 632
PctI GAATGC 1 cut(s) 644
PfeI GAWTC 3 cut(s) 478, 906, 965
PflFI GACNNNGTC 1 cut(s) 1079
PkrI GCNGC 8 cut(s) 141, 286, 430, 448, 451, 739, 814, 1260
PleI GAGTC 1 cut(s) 428
PpsI GAGTC 1 cut(s) 428
Psp6I CCWGG 4 cut(s) 213, 241, 486, 1134
PspGI CCWGG 4 cut(s) 213, 241, 486, 1134
PspN4I GGNNCC 4 cut(s) 30, 342, 1171, 1291
PspPI GGNCC 1 cut(s) 28
PstI CTGCAG 1 cut(s) 382
PsuI RGATCY 1 cut(s) 1283
PsyI GACNNNGTC 1 cut(s) 1079
PvuII CAGCTG 1 cut(s) 1258
RsaI GTAC 6 cut(s) 251, 556, 787, 962, 1124, 1280
RsaNI GTAC 6 cut(s) 250, 555, 786, 961, 1123, 1279
RseI CAYNNNNRTG 1 cut(s) 329
SaqAI TTAA 1 cut(s) 1297
SatI GCNGC 8 cut(s) 140, 285, 429, 447, 450, 738, 813, 1259
Sau3AI GATC 2 cut(s) 498, 1283
Sau96I GGNCC 1 cut(s) 28
SchI GAGTC 1 cut(s) 429
ScrFI CCNGG 4 cut(s) 215, 243, 488, 1136
SfaNI GCATC 4 cut(s) 362, 793, 1070, 1129
SfcI CTRYAG 3 cut(s) 378, 859, 1253
SmiMI CAYNNNNRTG 1 cut(s) 329
SphI GCATGC 1 cut(s) 315
Sse9I AATT 5 cut(s) 456, 836, 885, 927, 1071
SseBI AGGCCT 1 cut(s) 632
SsiI CCGC 3 cut(s) 126, 417, 1084
SspMI CTAG 1 cut(s) 1017
StuI AGGCCT 1 cut(s) 632
StyD4I CCNGG 4 cut(s) 213, 241, 486, 1134
TaaI ACNGT 4 cut(s) 902, 941, 960, 1081
TaiI ACGT 1 cut(s) 262
TasI AATT 5 cut(s) 456, 836, 885, 927, 1071
TatI WGTACW 1 cut(s) 1122
TfiI GAWTC 3 cut(s) 478, 906, 965
Tru1I TTAA 1 cut(s) 1297
Tru9I TTAA 1 cut(s) 1297
TscAI CASTG 3 cut(s) 331, 604, 1084
TseI GCWGC 8 cut(s) 139, 284, 428, 446, 449, 737, 812, 1258
TspDTI ATGAA 2 cut(s) 292, 570
TspGWI ACGGA 4 cut(s) 72, 602, 1199, 1296
TspRI CASTG 3 cut(s) 331, 604, 1084
Tth111I GACNNNGTC 1 cut(s) 1079
XceI RCATGY 1 cut(s) 315
XcmI CCANNNNNNNNNTGG 1 cut(s) 185
XmiI GTMKAC 3 cut(s) 765, 858, 936
XspI CTAG 1 cut(s) 1017
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.